FastQCFastQC Report
Thu 26 May 2016
SRR936357_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936357_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692439
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38430.14273303870579798No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27710.10291783769288737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49400.040.539791
GGTATCA29050.038.4621851
TCAACGC58750.033.1128964
CAACGCA60450.032.181685
ATCAACG60550.032.1291283
AACGCAG64400.030.2784926
TATCAAC66750.029.499412
TACATGG88050.026.8789272
GTACATG91250.026.7722531
ACATGGG87000.026.6592623
ACGCAGA77100.025.168217
CGCAGAG78950.024.7592188
TACCTGG25400.024.03962
GTACCTG25900.023.7635691
CATGGGG59100.023.466234
GAGTACA60750.023.068191
GTATAGG7100.020.6714741
AGTACAT61900.020.0346572
GCAGAGT97750.019.9005039
AGAGTAC78300.019.314910-11
Produced by FastQC (version 0.11.5)