##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936357_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2692439 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.633168290906497 33.0 31.0 34.0 30.0 34.0 2 31.7190023617991 33.0 31.0 34.0 30.0 34.0 3 31.792063255657787 34.0 31.0 34.0 30.0 34.0 4 35.27618044457089 37.0 35.0 37.0 33.0 37.0 5 35.255608390756485 37.0 35.0 37.0 33.0 37.0 6 35.33989887978892 37.0 35.0 37.0 33.0 37.0 7 35.27960187770271 37.0 35.0 37.0 33.0 37.0 8 35.27867335155968 37.0 35.0 37.0 33.0 37.0 9 36.93859730898267 39.0 37.0 39.0 33.0 39.0 10-11 36.85952755104201 39.0 37.0 39.0 33.0 39.0 12-13 36.75622307506317 39.0 37.0 39.0 33.0 39.0 14-15 38.00186020927494 40.0 38.0 41.0 33.0 41.0 16-17 37.93620264005981 40.0 38.0 41.0 33.0 41.0 18-19 37.866544794515306 40.0 38.0 41.0 33.0 41.0 20-21 37.77150011569436 40.0 38.0 41.0 32.0 41.0 22-23 37.61165823998241 40.0 38.0 41.0 32.0 41.0 24-25 37.769175271937456 40.0 38.0 41.0 32.5 41.0 26-27 37.72205814133579 40.0 38.0 41.0 32.0 41.0 28-29 37.59483353197602 40.0 38.0 41.0 32.0 41.0 30-31 37.44645171162652 40.0 37.5 41.0 32.0 41.0 32-33 37.275891487235185 40.0 37.0 41.0 31.0 41.0 34-35 37.008930378738384 40.0 37.0 41.0 30.5 41.0 36-37 36.81459022841372 40.0 37.0 41.0 30.0 41.0 38-39 36.65106526090285 40.0 36.0 41.0 30.0 41.0 40-41 36.46596320288036 40.0 36.0 41.0 30.0 41.0 42-43 36.28196014840076 40.0 36.0 41.0 29.5 41.0 44-45 36.103434655344095 39.5 35.0 41.0 29.0 41.0 46-47 35.85213982563765 39.0 35.0 41.0 28.0 41.0 48-49 35.695058086738456 39.0 35.0 41.0 27.5 41.0 50-51 35.38512348840587 38.5 35.0 40.5 27.5 41.0 52-53 35.39683016031189 39.0 35.0 40.5 27.5 41.0 54-55 35.46699275266775 39.0 35.0 41.0 27.5 41.0 56-57 35.28922363700718 39.0 35.0 41.0 27.0 41.0 58-59 35.0320194812213 38.5 35.0 40.5 26.5 41.0 60-61 34.719227993651856 38.0 34.0 40.0 26.0 41.0 62-63 34.39959326840831 37.0 34.0 40.0 26.0 41.0 64-65 34.0521415712668 37.0 34.0 40.0 26.0 41.0 66-67 33.68068097364509 36.0 34.0 39.0 25.0 41.0 68-69 33.28992541706609 36.0 33.0 39.0 23.5 41.0 70-71 32.87839873066763 35.0 33.0 38.5 22.5 40.5 72-73 32.47591830307019 35.0 33.0 37.5 22.0 40.0 74-75 32.06377024697681 35.0 32.0 37.0 20.5 39.0 76-77 31.680037876438426 35.0 32.0 36.5 20.0 39.0 78-79 31.292186378224354 35.0 32.0 36.0 18.0 38.5 80-81 30.913853758618117 35.0 31.0 35.5 17.0 37.0 82-83 30.581591263534662 35.0 31.0 35.0 12.0 37.0 84-85 30.28409185871992 34.5 31.0 35.0 7.5 36.5 86-87 29.972748129112674 34.0 31.0 35.0 4.5 36.0 88-89 29.651792668283292 34.0 30.5 35.0 2.0 36.0 90-91 29.30274074918689 34.0 30.0 35.0 2.0 35.0 92-93 28.992169181920183 34.0 30.0 35.0 2.0 35.0 94-95 28.609395421771858 34.0 29.0 35.0 2.0 35.0 96-97 28.18376442326084 34.0 29.0 35.0 2.0 35.0 98-99 27.674015455874766 34.0 29.0 35.0 2.0 35.0 100-101 24.391255660759633 30.0 14.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26966810373816585 1101 2 0.2569450602344432 1101 3 0.2990668505369456 1101 4 0.10479883975868631 1101 5 0.2619565099405392 1101 6 0.24904159156275085 1101 7 0.34397082832312265 1101 8 0.24496947519648415 1101 9 0.33978056792216904 1101 10-11 0.495583774284178 1101 12-13 0.4435865502285097 1101 14-15 0.6506022184907962 1101 16-17 0.5843365458271705 1101 18-19 0.6358785226832282 1101 20-21 0.629833159562672 1101 22-23 0.7018094958847385 1101 24-25 0.4756667500943408 1101 26-27 0.5015316721241732 1101 28-29 0.46342067800371467 1101 30-31 0.5158018877901682 1101 32-33 0.5417656206597528 1101 34-35 0.4490330808883911 1101 36-37 0.5834182998636592 1101 38-39 0.5536928721206849 1101 40-41 0.5240392678204415 1101 42-43 0.4493482843334604 1101 44-45 0.44003199889133526 1101 46-47 0.489049852428316 1101 48-49 0.4206131382038336 1101 50-51 0.5494699086672981 1101 52-53 0.6928908350722693 1101 54-55 0.6770945122979697 1101 56-57 0.6303643640913421 1101 58-59 0.5809218798056364 1101 60-61 0.6128678054357977 1101 62-63 0.5752809196604858 1101 64-65 0.4867860950901459 1101 66-67 0.6116726196293101 1101 68-69 0.5066434747386523 1101 70-71 0.7284248140082639 1101 72-73 0.8662697971851756 1101 74-75 0.6832467025213376 1101 76-77 0.5894703601415081 1101 78-79 0.7579412552728719 1101 80-81 0.6675897299583688 1101 82-83 0.781256527983242 1101 84-85 0.5762454916209094 1101 86-87 0.614566165877207 1101 88-89 0.4762755201254443 1101 90-91 0.7217834119039601 1101 92-93 0.6225474222914897 1101 94-95 0.7723549737047684 1101 96-97 0.8677437515379047 1101 98-99 1.0207699907282866 1101 100-101 1.0061557885790329 1102 1 0.31904321616529785 1102 2 0.274002081112819 1102 3 0.14997684790899157 1102 4 0.12187875161220774 1102 5 0.2533401306006695 1102 6 0.22575209715049027 1102 7 0.13148345891433166 1102 8 0.12296219983175405 1102 9 0.15576272233423794 1102 10-11 0.2559268000529258 1102 12-13 0.28452527487315393 1102 14-15 0.4048631842592343 1102 16-17 0.4106691160078384 1102 18-19 0.35001611510779185 1102 20-21 0.23490507868073962 1102 22-23 0.22294282265720256 1102 24-25 0.28842578310744926 1102 26-27 0.22265542794559678 1102 28-29 0.20359720527335412 1102 30-31 0.2954763420747284 1102 32-33 0.06612594407499017 1102 34-35 0.07892025902234678 1102 36-37 0.12375805600807865 1102 38-39 0.02971358430759352 1102 40-41 0.06701022440004323 1102 42-43 0.15669870262546937 1102 44-45 0.03220899912096087 1102 46-47 0.247069444219143 1102 48-49 0.31730878087967795 1102 50-51 0.17114202050215255 1102 52-53 0.3322844369185418 1102 54-55 0.30313150050893967 1102 56-57 0.35971742547989294 1102 58-59 0.29984566871983986 1102 60-61 0.24282347625132417 1102 62-63 0.4023470825094009 1102 64-65 0.2879393135792796 1102 66-67 0.33772992132767854 1102 68-69 0.2854589767233193 1102 70-71 0.38500548270591395 1102 72-73 0.3942039065045435 1102 74-75 0.4317648273070773 1102 76-77 0.43290636400449856 1102 78-79 0.31924788294918116 1102 80-81 0.3656638058749344 1102 82-83 0.5572695077068808 1102 84-85 0.5604966809602772 1102 86-87 0.371302330986385 1102 88-89 0.24878942504102142 1102 90-91 0.2299316978385555 1102 92-93 0.35268715817650076 1102 94-95 0.4453216152454367 1102 96-97 0.5368257765709004 1102 98-99 0.6063210449928746 1102 100-101 0.7262417901529226 1103 1 0.3308070115773738 1103 2 0.32422228761765837 1103 3 0.35956153556686843 1103 4 0.25478140195045285 1103 5 0.27261283105368506 1103 6 0.24886642205195386 1103 7 0.3454402472615854 1103 8 0.3076221219708586 1103 9 0.37563439002065024 1103 10-11 0.40484069150128477 1103 12-13 0.4770265153634412 1103 14-15 0.5108747231562631 1103 16-17 0.5121089330974158 1103 18-19 0.3707549833304995 1103 20-21 0.4544275392594699 1103 22-23 0.4414799028425662 1103 24-25 0.4966844532949253 1103 26-27 0.48801803027089363 1103 28-29 0.4408114120642068 1103 30-31 0.49166226291672643 1103 32-33 0.5694848766958458 1103 34-35 0.4141057577687022 1103 36-37 0.46721865516230565 1103 38-39 0.5628354542986713 1103 40-41 0.5749413116581863 1103 42-43 0.4783691764633389 1103 44-45 0.6199234360041572 1103 46-47 0.61592243824159 1103 48-49 0.6505764106446605 1103 50-51 0.5910275560563534 1103 52-53 0.7390173992647675 1103 54-55 0.7444991596717188 1103 56-57 0.7789294778169804 1103 58-59 0.7568875676862845 1103 60-61 0.7189587975547553 1103 62-63 0.671166177299483 1103 64-65 0.6819185986898262 1103 66-67 0.7712227611103515 1103 68-69 0.7052601104604648 1103 70-71 0.7618043667667322 1103 72-73 0.6492126133094231 1103 74-75 0.5943093957947738 1103 76-77 0.48564390880671127 1103 78-79 0.5212912033287829 1103 80-81 0.5329086466299167 1103 82-83 0.666232946051661 1103 84-85 0.7843334161629514 1103 86-87 0.8229142065903972 1103 88-89 0.7810045165360542 1103 90-91 0.925530614863689 1103 92-93 0.9912088053814081 1103 94-95 0.9732224321165184 1103 96-97 1.0118320179770635 1103 98-99 0.957683904938488 1103 100-101 0.922702817635539 1104 1 0.27442993303287366 1104 2 0.15312883493768936 1104 3 0.22441009422279734 1104 4 0.14395952376318633 1104 5 0.2778352613206465 1104 6 0.17721530799776986 1104 7 0.2767103857381912 1104 8 0.20427310916849706 1104 9 0.23821424621606724 1104 10-11 0.3447335230916124 1104 12-13 0.3384829299915211 1104 14-15 0.4377142699232053 1104 16-17 0.49235965237402723 1104 18-19 0.3544496041055396 1104 20-21 0.40951271074414564 1104 22-23 0.5138421746910709 1104 24-25 0.43454301332091205 1104 26-27 0.3827978834217589 1104 28-29 0.479627485295957 1104 30-31 0.5099803480932863 1104 32-33 0.5667163594458984 1104 34-35 0.5805618425697645 1104 36-37 0.5787613322413492 1104 38-39 0.49712858425558437 1104 40-41 0.5837124269301981 1104 42-43 0.6104509431183303 1104 44-45 0.5054699086310563 1104 46-47 0.5058786981046879 1104 48-49 0.4535544949078485 1104 50-51 0.5256517815971407 1104 52-53 0.4267257611408297 1104 54-55 0.5580276491147202 1104 56-57 0.7035990165158452 1104 58-59 0.6112570145410032 1104 60-61 0.590783197650147 1104 62-63 0.6087679796772818 1104 64-65 0.5931837136472282 1104 66-67 0.651477793509514 1104 68-69 0.618716037959409 1104 70-71 0.5303677925023749 1104 72-73 0.5994346122641261 1104 74-75 0.5528143660342337 1104 76-77 0.5423008328906782 1104 78-79 0.5725714395864117 1104 80-81 0.5734532671512476 1104 82-83 0.7557109567380529 1104 84-85 0.5849146728546337 1104 86-87 0.6244007115910861 1104 88-89 0.6614681010960872 1104 90-91 0.6520525128405801 1104 92-93 0.5209148026514541 1104 94-95 0.32135865574938904 1104 96-97 0.6448794166567424 1104 98-99 0.8258543290960354 1104 100-101 1.0454398237195797 1105 1 0.20893904531976304 1105 2 0.24245864093416358 1105 3 0.08897505600997846 1105 4 0.08002654316354096 1105 5 0.15474590212017603 1105 6 0.11920531387666244 1105 7 0.17258046216382184 1105 8 0.25960820617026314 1105 9 0.23476039553999328 1105 10-11 0.2380756747670958 1105 12-13 0.24435447614084893 1105 14-15 0.24147676257457817 1105 16-17 0.2540660015803766 1105 18-19 0.2400169174506317 1105 20-21 0.3300814938129264 1105 22-23 0.414349229364781 1105 24-25 0.3802587687588854 1105 26-27 0.3482814225164077 1105 28-29 0.4057444753018373 1105 30-31 0.4282196191103367 1105 32-33 0.4842060713800507 1105 34-35 0.20174056097070547 1105 36-37 0.38927720525722265 1105 38-39 0.41575733205100107 1105 40-41 0.3790681000636482 1105 42-43 0.34809056686906814 1105 44-45 0.3967250526639745 1105 46-47 0.32278052056206974 1105 48-49 0.3240424431735818 1105 50-51 0.3856826457946738 1105 52-53 0.38413316854823165 1105 54-55 0.43245445687486495 1105 56-57 0.5374761476151946 1105 58-59 0.6364848217132462 1105 60-61 0.5398058313950074 1105 62-63 0.36727767985365034 1105 64-65 0.2855484990675734 1105 66-67 0.2711941368369679 1105 68-69 0.3184588363133187 1105 70-71 0.3178898383577575 1105 72-73 0.336655364762656 1105 74-75 0.41441048596367835 1105 76-77 0.46119413095064843 1105 78-79 0.3670379298862372 1105 80-81 0.3818453894322609 1105 82-83 0.3557109567380543 1105 84-85 0.3067694641538701 1105 86-87 0.3291626163529884 1105 88-89 0.22038197528774006 1105 90-91 0.3044996027876721 1105 92-93 0.2628827626749697 1105 94-95 0.2918098609228146 1105 96-97 0.4362962302957776 1105 98-99 0.5584895436756945 1105 100-101 0.6795374133727243 1106 1 0.14405181405594902 1106 2 0.1388219119809797 1106 3 0.16951805379981266 1106 4 0.15671053954689995 1106 5 0.18102553844727964 1106 6 0.22363694940007406 1106 7 0.18606373521988218 1106 8 0.11895118386767223 1106 9 0.22735382531696757 1106 10-11 0.24453476156637777 1106 12-13 0.2538971223132407 1106 14-15 0.26128224278354395 1106 16-17 0.29363866081284584 1106 18-19 0.12340487706485703 1106 20-21 0.16580519785150472 1106 22-23 0.2002707182134955 1106 24-25 0.13887214026782857 1106 26-27 0.1918116616162706 1106 28-29 0.18913808591063486 1106 30-31 0.24268679311073527 1106 32-33 0.21513522046584654 1106 34-35 0.15911008578485308 1106 36-37 0.19795085404162904 1106 38-39 0.12974354362423668 1106 40-41 0.12342338437147049 1106 42-43 0.30912130827955053 1106 44-45 0.2984040259410463 1106 46-47 0.27673433309973916 1106 48-49 0.15684081568713992 1106 50-51 0.19137005789031747 1106 52-53 0.2267579927395147 1106 54-55 0.24501975725662106 1106 56-57 0.22131304735399482 1106 58-59 0.12955544658767337 1106 60-61 0.16533765277334567 1106 62-63 0.11329749600795225 1106 64-65 0.09145818361650271 1106 66-67 0.08703575829567001 1106 68-69 0.14680799243146225 1106 70-71 0.14954608207887787 1106 72-73 0.22137130810201455 1106 74-75 0.19789953559197926 1106 76-77 0.15379383961048276 1106 78-79 0.20812631144139004 1106 80-81 0.2246642311039473 1106 82-83 0.15139924787729342 1106 84-85 0.1761209811319695 1106 86-87 0.1770598184827854 1106 88-89 0.16575946172535438 1106 90-91 0.19569409746317845 1106 92-93 0.3094934217065166 1106 94-95 0.28100892662504506 1106 96-97 0.24877927731205318 1106 98-99 0.3132162866935779 1106 100-101 0.35600478829737625 1107 1 -0.06335830160947253 1107 2 0.0896973905919296 1107 3 0.2009010197764951 1107 4 0.00437596320080047 1107 5 -0.06202911106838371 1107 6 0.09036360330377846 1107 7 0.09389634653187073 1107 8 0.20571023553901568 1107 9 0.2224815125943067 1107 10-11 0.21701521440289184 1107 12-13 0.21668279114376077 1107 14-15 0.231155963381255 1107 16-17 -0.010956853024929103 1107 18-19 0.24786394351389163 1107 20-21 0.3450445825402184 1107 22-23 0.2693522844362022 1107 24-25 0.36546763213875977 1107 26-27 0.3069974744960291 1107 28-29 0.24456842305510662 1107 30-31 0.2483899351683263 1107 32-33 0.21999114126272445 1107 34-35 0.15843951456701433 1107 36-37 0.19337592579999097 1107 38-39 0.23449568772141305 1107 40-41 0.33445237128721317 1107 42-43 -0.05049258115665367 1107 44-45 0.2456651265199099 1107 46-47 0.3368939488530245 1107 48-49 0.39251410613408666 1107 50-51 0.3089723901035555 1107 52-53 0.4246651182514043 1107 54-55 0.4495134414559345 1107 56-57 0.36005846759969984 1107 58-59 0.36179491980941947 1107 60-61 0.3502677815656199 1107 62-63 0.5653399632440781 1107 64-65 0.388405324731508 1107 66-67 0.3672969174527907 1107 68-69 0.4371466080143449 1107 70-71 0.5086997494768823 1107 72-73 0.33350788995447544 1107 74-75 0.27603094258834915 1107 76-77 0.39545658996594213 1107 78-79 0.36615371208468517 1107 80-81 0.44180108254949957 1107 82-83 0.5616428036038492 1107 84-85 0.4758349461873763 1107 86-87 0.2510846736347432 1107 88-89 0.56602549352802 1107 90-91 0.6563677873073068 1107 92-93 0.39948143313459994 1107 94-95 0.5076323762983357 1107 96-97 0.6336368156674936 1107 98-99 0.5688368034602718 1107 100-101 0.5848191811071608 1108 1 0.06977936724794986 1108 2 0.15728804676775354 1108 3 0.12410172617849113 1108 4 0.12383733488228188 1108 5 0.1910432751330262 1108 6 0.06692051127805598 1108 7 0.0788824238005148 1108 8 0.11414777399434684 1108 9 0.21365468813721833 1108 10-11 0.12531009306845675 1108 12-13 0.08150715958014842 1108 14-15 0.07824643177102786 1108 16-17 0.13892361839405964 1108 18-19 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#Quality Count 2 25834.0 3 5554.0 4 4770.0 5 3823.0 6 3654.0 7 4046.0 8 4831.0 9 5821.0 10 6644.0 11 7829.0 12 9737.0 13 13687.0 14 13889.0 15 11690.0 16 13186.0 17 15727.0 18 14951.0 19 12287.0 20 12377.0 21 13020.0 22 13948.0 23 15298.0 24 17775.0 25 20369.0 26 24292.0 27 28989.0 28 34924.0 29 42503.0 30 51683.0 31 63736.0 32 81401.0 33 106543.0 34 147581.0 35 222352.0 36 372683.0 37 602317.0 38 544302.0 39 98308.0 40 78.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.280391667970314 16.991465009287825 11.8298560804888 24.898287242253055 2 16.454044866023008 18.58082005747178 38.74029383708802 26.224841239417202 3 20.832184939561678 21.857047448039978 27.41235124431406 29.898416368084284 4 13.079805963718483 14.176162954818963 34.780186310487764 37.96384477097479 5 14.735657792052702 36.59921157739865 33.3928507089526 15.272279921596041 6 36.30218422034978 33.41086408655909 15.987653518505793 14.29929817458534 7 31.732885334181194 30.361847469066035 18.98912700709105 18.916140189661725 8 28.413354996348637 34.329564592707825 18.443799110449433 18.813281300494104 9 26.949517087667164 15.389190193164934 18.500594353640416 39.160698365527495 10-11 26.832420382354865 25.324918436112746 25.996371966982224 21.846289214550165 12-13 26.84657006790441 21.987510433045202 26.85069314050726 24.31522635854314 14-15 23.36908445638687 23.78324749510294 24.306894440241937 28.540773608268253 16-17 24.151192431797092 26.920766568074374 25.03484535423757 23.89319564589096 18-19 24.100488800383047 25.871429043709725 26.482253080400774 23.545829075506454 20-21 24.42437213096061 25.242049517869525 26.76833483080212 23.565243520367744 22-23 24.012304658988295 25.087708676073518 26.64241344560539 24.25757321933279 24-25 23.968193261100904 25.219985961168646 26.950711219678738 23.86110955805172 26-27 23.743776548564092 25.572070947717805 26.70443824389838 23.97971425981972 28-29 23.7187269217796 25.93579629797484 26.609921795944324 23.73555498430124 30-31 23.80639286097031 25.54532964762315 26.610459772100377 24.037817719306165 32-33 23.641811924087932 25.38829982743881 26.76215882204467 24.20772942642859 34-35 24.02818808491654 25.691249345049883 26.585779938994214 23.694782631039367 36-37 24.07896894748557 25.384763067223385 26.550997840001926 23.985270145289117 38-39 23.828217118362254 25.626833971647507 26.631861628611595 23.91308728137864 40-41 24.051791300875287 25.439915354420705 26.742068908891238 23.76622443581277 42-43 23.946239263675473 25.381443660605264 26.513590388134737 24.158726687584526 44-45 23.94162994978138 25.66718359562346 26.584816471558643 23.806369983036518 46-47 24.160744403267458 25.85385946062565 26.289119925437245 23.696276210669648 48-49 24.200899691256062 25.81806318145491 26.21432376268189 23.766713364607142 50-51 23.97847033744862 26.046561115196948 26.232197055957712 23.742771491396724 52-53 24.318311957947515 25.823927242810395 26.135300630324405 23.722460168917685 54-55 24.048704848669317 25.886996196554936 26.202267286957344 23.862031667818407 56-57 23.938003538837453 26.20880111337902 26.056174286065108 23.797021061718414 58-59 24.030576970983976 25.86888999400067 26.10095102851593 23.999582006499427 60-61 23.756515767995275 26.02541651000212 26.342427807576435 23.87563991442617 62-63 23.861022131421162 25.986296868588727 26.492173384412972 23.660507615577135 64-65 23.746988133933538 26.018715699812013 26.269929264187425 23.96436690206702 66-67 23.68604140254841 26.273798629243732 26.14778681726888 23.89237315093898 68-69 23.951133747301657 25.88772616206276 26.404803988368876 23.75633610226671 70-71 23.784736702359407 25.88875933344939 26.216168257623636 24.110335706567565 72-73 23.840823689431048 26.088462343016204 26.137446157457678 23.933267810095067 74-75 23.70953874085254 26.30269488843322 26.066831262325778 23.920935108388463 76-77 24.009579647615418 26.159738048812986 25.926498186834852 23.904184116736747 78-79 23.956580024766204 26.274660770946962 26.034220353519256 23.73453885076758 80-81 24.01281419915081 26.095989301968764 26.026084848629615 23.865111650250814 82-83 23.952560097634162 26.268796255864206 26.05604260671272 23.722601039788906 84-85 23.8429884930061 26.40816191713688 25.891492050091617 23.857357539765403 86-87 23.933834266557064 26.25028808481217 26.045330320145037 23.770547328485733 88-89 23.859485142926786 26.423206773183715 25.768479118797792 23.948828965091707 90-91 23.748919796790183 26.47503626813197 25.94109991896026 23.834944016117593 92-93 23.886277385860478 26.29582058510651 26.049184278368077 23.768717750664937 94-95 23.866343683246345 26.066411646362642 26.210343419007543 23.856901251383476 96-97 24.010033151562528 25.9459948546984 26.05533717769573 23.98863481604334 98-99 23.594309017251692 26.160340150178403 25.92676951534793 24.318581317221977 100-101 23.798320525468224 26.093102408694524 25.906394629502216 24.202182436335036 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 47.0 1 47.5 2 109.5 3 191.5 4 169.5 5 175.5 6 313.5 7 565.5 8 901.5 9 1063.0 10 1052.5 11 1092.5 12 1263.0 13 1536.5 14 1770.5 15 1979.0 16 2247.0 17 2470.5 18 2637.0 19 2769.0 20 2926.5 21 3201.0 22 3575.0 23 4222.0 24 5264.5 25 6606.5 26 8650.5 27 11429.5 28 14070.0 29 16771.0 30 19996.0 31 24275.5 32 30805.5 33 34513.0 34 36537.5 35 40594.0 36 45607.5 37 50924.0 38 55170.0 39 58783.0 40 62861.5 41 67388.5 42 71020.5 43 75637.0 44 81225.0 45 86690.0 46 90938.5 47 95536.5 48 100221.0 49 102755.0 50 102875.5 51 103633.5 52 107316.0 53 110898.5 54 113562.0 55 109480.0 56 102071.0 57 95978.5 58 90954.0 59 83550.0 60 72144.5 61 62046.0 62 51829.0 63 40282.0 64 29131.5 65 21275.0 66 15790.5 67 12119.5 68 9376.0 69 7530.5 70 5963.5 71 3914.5 72 2735.5 73 2178.0 74 1652.0 75 1156.5 76 797.0 77 460.0 78 268.5 79 167.0 80 114.0 81 83.0 82 49.5 83 38.0 84 34.5 85 32.5 86 31.0 87 24.5 88 24.0 89 25.5 90 30.0 91 29.5 92 26.0 93 29.5 94 29.0 95 25.0 96 29.0 97 28.5 98 17.0 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04761482061432032 2 0.012442250316534563 3 0.010139505481832643 4 0.005905426269638792 5 0.008468158424387702 6 0.006462541955453773 7 0.006388259864011776 8 0.010882326396252617 9 0.016304919071518424 10-11 0.002637014246190907 12-13 0.010102364436111646 14-15 0.009749524501762157 16-17 0.02024186991794429 18-19 0.012553673453697558 20-21 0.004382643395077846 22-23 0.010696621167647623 24-25 0.02300887782415869 26-27 0.007539632281362734 28-29 0.01883051018054634 30-31 0.01561780972567995 32-33 0.013017936525210041 34-35 0.26444424553351065 36-37 0.467067220464419 38-39 0.4628888528208067 40-41 0.4840035373131945 42-43 0.47358547398845435 44-45 0.48855331541401675 46-47 0.49596295403535606 48-49 0.5021469381479023 50-51 0.512880700361271 52-53 0.5014412582792034 54-55 0.48868330907404034 56-57 0.4838735436531709 58-59 0.4817750745699345 60-61 0.4784323804550446 62-63 0.47661246921471573 64-65 0.4919145800517672 66-67 0.46543301445269514 68-69 0.4920631442346512 70-71 0.47401259601424584 72-73 0.4821836260728655 74-75 0.4957772488067511 76-77 0.4831492932616115 78-79 0.482127914504284 80-81 0.48534061495915043 82-83 0.484653505613312 84-85 0.48537775600487143 86-87 0.4867891157422694 88-89 0.500011328018945 90-91 0.524561559240525 92-93 0.5124907193812004 94-95 0.48448637090756747 96-97 0.45772624746558793 98-99 0.44916523642689765 100-101 0.45739197805409887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2692439.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.24224395379204 #Duplication Level Percentage of deduplicated Percentage of total 1 74.01415133612633 38.666653501347945 2 14.067593461860314 14.698452989545533 3 4.90213059453213 7.6829490723868545 4 2.3842686787551393 4.982381838676457 5 1.2767359883950287 3.3349776485159444 6 0.7787267566310225 2.4409459915957874 7 0.4956389039146218 1.8125301971908436 8 0.3403686316388882 1.4225296870637745 9 0.24588614992345098 1.156107980624365 >10 1.3302267591639112 12.679077237189688 >50 0.10481260218376767 3.7668481608874558 >100 0.05525961587352226 5.106791777006396 >500 0.002919006119991203 1.0460814545781203 >1k 0.001281514881938015 1.2036724633907743 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3843 0.14273303870579798 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2771 0.10291783769288737 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4856418288399477E-4 2 0.0 0.0 0.0 0.0 1.4856418288399477E-4 3 0.0 0.0 0.0 0.0 1.4856418288399477E-4 4 0.0 0.0 0.0 0.0 1.4856418288399477E-4 5 0.0 0.0 0.0 0.0 1.4856418288399477E-4 6 0.0 0.0 0.0 0.0 1.4856418288399477E-4 7 0.0 0.0 0.0 0.0 1.4856418288399477E-4 8 0.0 0.0 0.0 0.0 1.4856418288399477E-4 9 0.0 0.0 0.0 0.0 1.4856418288399477E-4 10-11 0.0 0.0 0.0 1.8570522860499346E-5 1.4856418288399477E-4 12-13 0.0 0.0 0.0 3.714104572099869E-5 4.0855150293098565E-4 14-15 0.0 0.0 0.0 3.714104572099869E-5 6.313977772569778E-4 16-17 0.0 0.0 0.0 1.8570522860499348E-4 8.5424405158297E-4 18-19 0.0 0.0 0.0 2.9712836576798954E-4 0.0010028082344669647 20-21 0.0 0.0 0.0 4.0855150293098565E-4 0.0010585198030484627 22-23 0.0 0.0 0.0 6.313977772569778E-4 0.0011328018944904601 24-25 0.0 0.0 0.0 9.285261430249674E-4 0.0012256545087929568 26-27 0.0 0.0 0.0 0.0010956608487694614 0.0013185071230954535 28-29 9.285261430249674E-5 0.0 0.0 0.001615635488863443 0.0014113597373979503 30-31 1.1142313716299608E-4 0.0 0.0 0.0031755594091453882 0.0014299302602584496 32-33 1.1142313716299608E-4 0.0 0.0 0.0057568620867547975 0.001559923920281945 34-35 1.1142313716299608E-4 0.0 0.0 0.009563819273157163 0.0016342060117239426 36-37 1.1142313716299608E-4 0.0 0.0 0.016694900051588912 0.0016342060117239426 38-39 1.1142313716299608E-4 0.0 0.0 0.027911495859330518 0.001764199671747438 40-41 1.1142313716299608E-4 0.0 0.0 0.04126370179602955 0.0017827701946079373 42-43 1.1142313716299608E-4 0.0 0.0 0.05409593309263459 0.0018570522860499347 44-45 1.1142313716299608E-4 0.0 0.0 0.06897092190389457 0.0019127638546314327 46-47 1.1142313716299608E-4 0.0 0.0 0.08692861750999745 0.0019684754232129308 48-49 1.1142313716299608E-4 0.0 0.0 0.10802473147952471 0.0020056164689339294 50-51 1.1142313716299608E-4 0.0 0.0 0.13281637949829134 0.0020056164689339294 52-53 1.1142313716299608E-4 0.0 0.0 0.15846227156864093 0.0021170396060969255 54-55 1.1142313716299608E-4 0.0 0.0 0.184646708801945 0.002191321697538923 56-57 1.1142313716299608E-4 0.0 0.0 0.21692227753349286 0.0022098922203994224 58-59 1.1142313716299608E-4 0.0 0.0 0.25406332325449155 0.0022284627432599216 60-61 1.1142313716299608E-4 0.0 0.0 0.2969798015851055 0.0022656037889809202 62-63 1.1142313716299608E-4 0.0 0.0 0.3377978108324831 0.0023770269261439163 64-65 1.1142313716299608E-4 0.0 0.0 0.38244134778912353 0.0024513090175859137 66-67 1.1142313716299608E-4 0.0 0.0 0.43351028565549676 0.002525591109027911 68-69 1.1142313716299608E-4 0.0 0.0 0.49215599684895367 0.0025627321547489097 70-71 1.1142313716299608E-4 0.0 0.0 0.5595112832639848 0.0025998732004699084 72-73 1.2999366002349543E-4 0.0 0.0 0.6293550197423228 0.002729866860493404 74-75 1.4856418288399477E-4 0.0 0.0 0.7001829939322674 0.0028041489519354014 76-77 1.4856418288399477E-4 0.0 0.0 0.7808904862839975 0.0028412899976563996 78-79 1.4856418288399477E-4 0.0 0.0 0.8771043652242446 0.002859860520516899 80-81 1.4856418288399477E-4 0.0 0.0 0.9849991030437458 0.0029898541805403944 82-83 2.2284627432599217E-4 0.0 0.0 1.0985021387671179 0.003305553069168884 84-85 2.2284627432599217E-4 0.0 0.0 1.2115594819418378 0.003305553069168884 86-87 2.414167971864915E-4 0.0 0.0 1.3373562037988604 0.0033984056834713804 88-89 2.5998732004699085E-4 0.0 0.0 1.4787893059044235 0.003528399343494876 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4940 0.0 40.53979 1 GGTATCA 2905 0.0 38.462185 1 TCAACGC 5875 0.0 33.112896 4 CAACGCA 6045 0.0 32.18168 5 ATCAACG 6055 0.0 32.129128 3 AACGCAG 6440 0.0 30.278492 6 TATCAAC 6675 0.0 29.49941 2 TACATGG 8805 0.0 26.878927 2 GTACATG 9125 0.0 26.772253 1 ACATGGG 8700 0.0 26.659262 3 ACGCAGA 7710 0.0 25.16821 7 CGCAGAG 7895 0.0 24.759218 8 TACCTGG 2540 0.0 24.0396 2 GTACCTG 2590 0.0 23.763569 1 CATGGGG 5910 0.0 23.46623 4 GAGTACA 6075 0.0 23.06819 1 GTATAGG 710 0.0 20.671474 1 AGTACAT 6190 0.0 20.034657 2 GCAGAGT 9775 0.0 19.900503 9 AGAGTAC 7830 0.0 19.3149 10-11 >>END_MODULE