FastQCFastQC Report
Thu 26 May 2016
SRR936357_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936357_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692439
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39710.1474870925580858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28300.10510915939042631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54200.040.5537571
GGTATCA31150.040.4740071
TCAACGC64050.033.7746054
ATCAACG65600.032.9765783
CAACGCA65900.032.8264585
TATCAAC69750.031.1575012
AACGCAG70000.031.0393076
GTACATG93200.026.0851541
TACATGG91900.025.7131442
ACATGGG87400.025.6197663
ACGCAGA85200.025.5574597
CGCAGAG87900.024.7724198
TAACGCA2052.708839E-823.1414594
GTGTAGC17900.021.5288281
GAGTACA62650.021.490861
GTACCTG25200.021.1449131
TAGACCG2701.0186341E-921.0844385
CATGGGG59400.020.5253834
GCGTATC1404.8019754E-420.3897361
TACCTGG23800.020.3359552
Produced by FastQC (version 0.11.5)