##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936357_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2692439 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.469077665269296 34.0 31.0 34.0 31.0 34.0 2 32.6052200254119 34.0 31.0 34.0 31.0 34.0 3 32.68925238417658 34.0 31.0 34.0 31.0 34.0 4 36.17857637628931 37.0 35.0 37.0 35.0 37.0 5 36.13552841865684 37.0 35.0 37.0 35.0 37.0 6 36.16867680196283 37.0 37.0 37.0 35.0 37.0 7 36.11705483392567 37.0 36.0 37.0 35.0 37.0 8 36.08996341235586 37.0 36.0 37.0 35.0 37.0 9 37.857638000340955 39.0 38.0 39.0 35.0 39.0 10-11 37.79198451664086 39.0 38.0 39.0 35.0 39.0 12-13 37.735819455891104 39.0 38.0 39.0 35.0 39.0 14-15 39.10182644806437 40.0 38.0 41.0 36.0 41.0 16-17 39.02196168604006 40.0 38.0 41.0 35.5 41.0 18-19 39.02513631692306 40.0 38.5 41.0 35.0 41.0 20-21 38.966947069181515 40.0 38.0 41.0 35.0 41.0 22-23 38.85200110383188 40.0 38.0 41.0 35.0 41.0 24-25 39.009481923267344 40.0 38.0 41.0 35.0 41.0 26-27 38.93206902737629 40.0 38.0 41.0 35.0 41.0 28-29 38.846671363770916 40.0 38.0 41.0 35.0 41.0 30-31 38.73796045147169 40.0 38.0 41.0 35.0 41.0 32-33 38.599978495334526 40.0 38.0 41.0 34.0 41.0 34-35 38.4665483600557 40.0 38.0 41.0 34.0 41.0 36-37 38.32935007998324 40.0 38.0 41.0 34.0 41.0 38-39 38.19511955516913 40.0 38.0 41.0 33.5 41.0 40-41 38.05299841519158 40.0 38.0 41.0 33.0 41.0 42-43 38.09138554299652 40.0 38.0 41.0 33.0 41.0 44-45 37.98163208154391 40.0 38.0 41.0 33.0 41.0 46-47 37.808955931777845 40.0 37.0 41.0 33.0 41.0 48-49 37.59301510637752 40.0 37.0 41.0 33.0 41.0 50-51 37.39038006060676 40.0 37.0 41.0 32.0 41.0 52-53 37.17178495037399 40.0 36.0 41.0 32.0 41.0 54-55 36.966873901321435 39.0 36.0 41.0 31.0 41.0 56-57 36.692666203394026 39.0 35.0 41.0 31.0 41.0 58-59 36.4058359353731 39.0 35.0 41.0 31.0 41.0 60-61 36.10829456117669 38.0 35.0 40.0 30.0 41.0 62-63 35.7947257115203 37.5 35.0 40.0 30.0 41.0 64-65 35.401458677429645 37.0 35.0 40.0 29.0 41.0 66-67 35.03714048860532 36.5 34.0 39.5 29.0 41.0 68-69 34.673511637589556 36.0 34.0 39.0 29.0 41.0 70-71 34.295731491038424 35.5 34.0 39.0 28.5 40.0 72-73 33.88898262876151 35.0 34.0 37.5 28.0 40.0 74-75 33.46735617780013 35.0 33.0 37.0 27.5 39.0 76-77 32.76686677024067 35.0 32.5 36.0 26.0 39.0 78-79 32.82766350509705 35.0 33.0 36.0 26.5 38.5 80-81 32.67352389413465 35.0 33.0 36.0 27.0 37.0 82-83 32.40279334090763 35.0 33.0 35.0 26.0 37.0 84-85 32.13186835430626 35.0 33.0 35.0 26.0 36.5 86-87 31.741369442353196 35.0 32.0 35.0 25.0 36.0 88-89 31.254487288291397 34.5 32.0 35.0 23.5 36.0 90-91 31.04040611505033 34.0 32.0 35.0 23.0 35.5 92-93 30.75248742868455 34.0 31.0 35.0 20.5 35.0 94-95 30.584065414295367 34.0 31.0 35.0 20.0 35.0 96-97 30.32291130829705 34.0 31.0 35.0 18.5 35.0 98-99 29.861683031630427 34.0 31.0 35.0 4.5 35.0 100-101 26.47734284787882 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15203142089657717 1101 2 0.13779777174349306 1101 3 0.2155599224726359 1101 4 0.13185339504725135 1101 5 0.018994083863596245 1101 6 0.12757586468610782 1101 7 0.15069051541527045 1101 8 0.08144410069075292 1101 9 0.13437006508684135 1101 10-11 0.11408750969026471 1101 12-13 0.23016519710163408 1101 14-15 0.2857015093523927 1101 16-17 0.3060978641817016 1101 18-19 0.30972320832673716 1101 20-21 0.22566113699542711 1101 22-23 0.26434535027032524 1101 24-25 0.1980433324197648 1101 26-27 0.2009946505710971 1101 28-29 0.2706529514416758 1101 30-31 0.26733716328386237 1101 32-33 0.3488318244678581 1101 34-35 0.32191462406556326 1101 36-37 0.33986637222131577 1101 38-39 0.44390924068553517 1101 40-41 0.4507382163738285 1101 42-43 0.3824827477099504 1101 44-45 0.46539256869454704 1101 46-47 0.49004520272564633 1101 48-49 0.39972113708396506 1101 50-51 0.3918820694497711 1101 52-53 0.5147038489531894 1101 54-55 0.5198004852830564 1101 56-57 0.4749547160064722 1101 58-59 0.4011413075136758 1101 60-61 0.5104595127358991 1101 62-63 0.5774802709970501 1101 64-65 0.7780219569654463 1101 66-67 0.6011444767600054 1101 68-69 0.6281211035187582 1101 70-71 0.6080162487211922 1101 72-73 0.518212290044815 1101 74-75 0.4950369923005269 1101 76-77 0.2671406856687355 1101 78-79 0.23745609371133725 1101 80-81 0.32875703853051164 1101 82-83 0.3524687289866648 1101 84-85 0.39541169213839567 1101 86-87 0.3981695171939883 1101 88-89 0.5478769394884218 1101 90-91 0.6468709487165505 1101 92-93 0.6706375475053505 1101 94-95 0.5712762729299676 1101 96-97 0.45411527873530133 1101 98-99 0.46295112423930007 1101 100-101 0.774443763641699 1102 1 0.05114224169484771 1102 2 0.05227835028314587 1102 3 0.0798739376253792 1102 4 0.0724796128200893 1102 5 0.06351757126580537 1102 6 0.036368666754157175 1102 7 0.07924670050268645 1102 8 0.1480927069187601 1102 9 0.014146122857169985 1102 10-11 0.03966381252020312 1102 12-13 0.2199112118189106 1102 14-15 0.19576512102916865 1102 16-17 0.19172463890819103 1102 18-19 0.23475220055786394 1102 20-21 0.11993772630501098 1102 22-23 0.17347232452681283 1102 24-25 0.2244933134761311 1102 26-27 0.21265165436233957 1102 28-29 0.09025521443987827 1102 30-31 0.09272840970286467 1102 32-33 0.15823471608467798 1102 34-35 0.11789502290205434 1102 36-37 0.09853303960895943 1102 38-39 0.05944332678949138 1102 40-41 0.03417005299787235 1102 42-43 0.07498872910373677 1102 44-45 0.13876484912953657 1102 46-47 0.1716822066538768 1102 48-49 0.2559188595941606 1102 50-51 0.12731484766794665 1102 52-53 0.1595922761773707 1102 54-55 0.19355839753960424 1102 56-57 0.2593230819044763 1102 58-59 0.1705547470034574 1102 60-61 0.0868253379158972 1102 62-63 0.15531400248915617 1102 64-65 0.17949226052140688 1102 66-67 0.12534834847104293 1102 68-69 -0.06965007252581046 1102 70-71 -0.01043932061701014 1102 72-73 -0.07856273204407671 1102 74-75 -0.08740404361162746 1102 76-77 -0.13310567169219212 1102 78-79 -0.08727176503801815 1102 80-81 -0.05375539198997359 1102 82-83 -7.112600472964914E-4 1102 84-85 -0.07913507556579447 1102 86-87 -0.06081742557749337 1102 88-89 0.010926129129877893 1102 90-91 0.17174271419472475 1102 92-93 0.12996722405046413 1102 94-95 0.11832155272076861 1102 96-97 0.032884385940409544 1102 98-99 0.014997631491052488 1102 100-101 0.3277779333067272 1103 1 0.004065418555001088 1103 2 0.029409526461854796 1103 3 0.12122217138534808 1103 4 -0.012829761823908825 1103 5 0.07228254847645132 1103 6 0.091445570227215 1103 7 0.12417080119482904 1103 8 0.16423016120759826 1103 9 0.08789738282480641 1103 10-11 0.17196335777186533 1103 12-13 0.19264146084149303 1103 14-15 0.21585266692211746 1103 16-17 0.2509039035199194 1103 18-19 0.2474633739041323 1103 20-21 0.2212534077990469 1103 22-23 0.22978867950406112 1103 24-25 0.26767215772353126 1103 26-27 0.20957795660014256 1103 28-29 0.25661327343700435 1103 30-31 0.2650151120277897 1103 32-33 0.23040008755135233 1103 34-35 0.20673724119303216 1103 36-37 0.22385086655389586 1103 38-39 0.20587234217779837 1103 40-41 0.23075564890586975 1103 42-43 0.2009071507222373 1103 44-45 0.21879162225156534 1103 46-47 0.32239320756074363 1103 48-49 0.34390511361125675 1103 50-51 0.23559155820278477 1103 52-53 0.24966182673726678 1103 54-55 0.28642853023830384 1103 56-57 0.3760132252611754 1103 58-59 0.2208267199561007 1103 60-61 0.1895352508616952 1103 62-63 0.21476066937051286 1103 64-65 0.2115506676176011 1103 66-67 0.1725906857382853 1103 68-69 0.15682727091011373 1103 70-71 -0.023414173896355805 1103 72-73 0.06870701200806195 1103 74-75 0.06665203936150732 1103 76-77 0.07060603455416725 1103 78-79 0.019687151793213786 1103 80-81 0.11346157452388184 1103 82-83 0.2603554196388984 1103 84-85 0.1032286843196033 1103 86-87 0.06507545908096901 1103 88-89 0.17652206136049742 1103 90-91 0.2808882873324059 1103 92-93 0.46523625895421006 1103 94-95 0.3589958877469819 1103 96-97 0.3370403140989673 1103 98-99 0.3838818758273028 1103 100-101 0.8158571072319489 1104 1 0.06639562106879282 1104 2 0.056548200963185025 1104 3 0.06915770796316423 1104 4 0.04623819776467286 1104 5 0.06885488461585254 1104 6 0.03252628651001288 1104 7 0.048360314725350406 1104 8 0.09871484454829016 1104 9 0.11460271536655853 1104 10-11 0.16576793780058807 1104 12-13 0.17137979638212641 1104 14-15 0.17449768678785915 1104 16-17 0.1239040100794142 1104 18-19 0.23795978893816283 1104 20-21 0.13365439626524278 1104 22-23 0.18650497245134545 1104 24-25 0.13924208358856305 1104 26-27 0.2704825452551063 1104 28-29 0.19607200192847785 1104 30-31 0.0603138084499264 1104 32-33 0.1796191586569833 1104 34-35 0.1615169421160303 1104 36-37 0.07128049922808799 1104 38-39 0.1255391661465879 1104 40-41 0.18320677107887207 1104 42-43 0.2231273432397316 1104 44-45 0.28801495573713254 1104 46-47 0.1900861436808654 1104 48-49 0.2574460349957093 1104 50-51 0.31848444560998956 1104 52-53 0.331907238336008 1104 54-55 0.2766920366547083 1104 56-57 0.29095312062990075 1104 58-59 0.24385777951270882 1104 60-61 0.2512636459234301 1104 62-63 0.22613767180107658 1104 64-65 0.08064587599216111 1104 66-67 -0.0077780101256834655 1104 68-69 0.25026751894003496 1104 70-71 0.18703017921137643 1104 72-73 0.11972287922478131 1104 74-75 0.20273917458550983 1104 76-77 0.1465679877390258 1104 78-79 0.13002570654196433 1104 80-81 0.3115858736414694 1104 82-83 0.3007138147513686 1104 84-85 0.2663116535746539 1104 86-87 0.2420294304519821 1104 88-89 0.2760765411725501 1104 90-91 0.35849633105224754 1104 92-93 0.3118479206227285 1104 94-95 0.22029474654550185 1104 96-97 0.13830837212822544 1104 98-99 0.17065251411869653 1104 100-101 0.14507800507183788 1105 1 0.04001408079836466 1105 2 0.017321275625143073 1105 3 -0.0045783296617614155 1105 4 0.06394836825262473 1105 5 0.04313472000679752 1105 6 0.028763793858637143 1105 7 0.08066484147432362 1105 8 0.1145731631843887 1105 9 0.02364151607202558 1105 10-11 0.07418210593697694 1105 12-13 0.10744681578248105 1105 14-15 -8.99138608971839E-4 1105 16-17 0.1053413998501398 1105 18-19 0.11957354555191557 1105 20-21 0.05323846446042779 1105 22-23 0.0032818683949074057 1105 24-25 0.07559569910884534 1105 26-27 0.08817066988767408 1105 28-29 -0.002377439576520146 1105 30-31 0.12188053390553222 1105 32-33 0.010174714212546121 1105 34-35 0.14430647768335092 1105 36-37 0.15803094014518848 1105 38-39 0.12058619730472486 1105 40-41 0.11320383163148762 1105 42-43 0.10592422742550411 1105 44-45 0.12408197513748576 1105 46-47 0.130995902646184 1105 48-49 0.1475841890227514 1105 50-51 0.04155910757354064 1105 52-53 0.10191017778339528 1105 54-55 0.21009150755418204 1105 56-57 0.0800551194541228 1105 58-59 0.12142244735515106 1105 60-61 0.23755112387757293 1105 62-63 0.2241902879092521 1105 64-65 0.25772553501626305 1105 66-67 0.13432957364856435 1105 68-69 0.17161525556554835 1105 70-71 0.1866994913806863 1105 72-73 0.15914821726123307 1105 74-75 0.10988203172836819 1105 76-77 0.09519379608705236 1105 78-79 0.015497487847078162 1105 80-81 0.13337452737457056 1105 82-83 0.14882521980721464 1105 84-85 0.2578093020167458 1105 86-87 0.16132690252723236 1105 88-89 0.32687754058465757 1105 90-91 0.2707538266430767 1105 92-93 0.3732823709460682 1105 94-95 0.28192026094879097 1105 96-97 0.2738021992887205 1105 98-99 0.19232358995644105 1105 100-101 0.3951441426379745 1106 1 -0.09930051634851367 1106 2 -0.09189361286189524 1106 3 -0.06020169715222323 1106 4 -0.02823567836180274 1106 5 -0.07298693962751912 1106 6 -0.043507403649904575 1106 7 -0.004277141648039162 1106 8 0.01895205609176287 1106 9 -0.07414454983113217 1106 10-11 -0.014058174352349795 1106 12-13 0.007021107524494141 1106 14-15 0.05509279930542732 1106 16-17 -0.07492344342895052 1106 18-19 -0.007362234034182791 1106 20-21 0.039651589837163215 1106 22-23 -0.012953439019199209 1106 24-25 0.005420392699363674 1106 26-27 -0.06584718725518002 1106 28-29 -0.09472473714534146 1106 30-31 -0.09649650045250979 1106 32-33 -0.052000039655247576 1106 34-35 0.05821483110910464 1106 36-37 -0.1379147851913558 1106 38-39 -0.05238007253654331 1106 40-41 -0.32453727747037675 1106 42-43 -0.2716049065909729 1106 44-45 -0.14496187658041748 1106 46-47 -0.10903009192886515 1106 48-49 -0.20810196729631514 1106 50-51 -0.050843444164449636 1106 52-53 -0.11701186360699722 1106 54-55 0.02748464096788439 1106 56-57 -0.04037322593466541 1106 58-59 0.0023773398606792284 1106 60-61 -0.008315487534922283 1106 62-63 -0.028169316269973876 1106 64-65 0.05521774920151756 1106 66-67 -0.11055627626101483 1106 68-69 -0.04889283163557678 1106 70-71 -0.12363554980878178 1106 72-73 -0.2089845685535181 1106 74-75 -0.029301964654997903 1106 76-77 -0.18965463168067487 1106 78-79 -0.30371149849007395 1106 80-81 -0.2808546769653901 1106 82-83 -0.16590189994363413 1106 84-85 -0.087462699188805 1106 86-87 -0.14442141473215742 1106 88-89 -0.1976408690998923 1106 90-91 -0.022885037130283337 1106 92-93 -0.09380937609030227 1106 94-95 -0.06430864200479292 1106 96-97 -0.03297962208961991 1106 98-99 -0.11592058324026411 1106 100-101 0.18833281051018247 1107 1 0.04541921999609855 1107 2 0.08592766222690784 1107 3 0.047749701536695 1107 4 0.07509169951585193 1107 5 0.07502599285305678 1107 6 0.06553950821112409 1107 7 0.08991319373907913 1107 8 0.089300377026035 1107 9 0.12829307098651554 1107 10-11 0.060678439851280075 1107 12-13 0.10320277066759331 1107 14-15 0.17832837907037913 1107 16-17 0.2002594638363533 1107 18-19 0.1064946679484251 1107 20-21 0.05459045877098845 1107 22-23 0.047199020868013974 1107 24-25 0.15958570585337384 1107 26-27 0.1972930630603429 1107 28-29 0.21230585135309354 1107 30-31 0.02770570368737424 1107 32-33 0.06972573557467143 1107 34-35 0.16192188592770407 1107 36-37 0.19660705983721272 1107 38-39 0.16594265569231936 1107 40-41 0.15833224479734298 1107 42-43 0.06337017102521258 1107 44-45 0.0026804486034350816 1107 46-47 -0.0027148071320581835 1107 48-49 0.009751219626892293 1107 50-51 0.031982543811032826 1107 52-53 0.06826449261267697 1107 54-55 0.11507219215728526 1107 56-57 -0.04377068137525697 1107 58-59 4.602334133423369E-4 1107 60-61 -0.01674717797016001 1107 62-63 -0.0032757223344148656 1107 64-65 -0.0791552704541516 1107 66-67 -0.03917093719943665 1107 68-69 0.02106075175588984 1107 70-71 0.2929183980991752 1107 72-73 0.22748407144584348 1107 74-75 0.06689567437515365 1107 76-77 -0.1678033625295754 1107 78-79 -0.018824903990037 1107 80-81 0.18249181206715548 1107 82-83 0.3393023284742753 1107 84-85 0.14225973974337336 1107 86-87 0.05166870324977069 1107 88-89 0.21163265073417392 1107 90-91 -0.05049544715142318 1107 92-93 0.21189379925038665 1107 94-95 0.3054337934126856 1107 96-97 0.038492752273342035 1107 98-99 -0.021075569314618292 1107 100-101 0.40558923148545745 1108 1 -0.02733167200764086 1108 2 0.027050214747390555 1108 3 0.09754556921945579 1108 4 0.07695243763744486 1108 5 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-0.7353457570389068 2316 54-55 -0.8672771537746655 2316 56-57 -0.8270719349074511 2316 58-59 -0.6142725238486619 2316 60-61 -0.8201992624126362 2316 62-63 -0.7784874055870219 2316 64-65 -0.6434737204971412 2316 66-67 -0.7067892632667281 2316 68-69 -0.5988001819959052 2316 70-71 -0.5588564153477904 2316 72-73 -0.7845289143583756 2316 74-75 -0.7652549257094776 2316 76-77 -0.6513489065397167 2316 78-79 -0.8207663834831678 2316 80-81 -0.7961292652929401 2316 82-83 -0.9281747099581992 2316 84-85 -0.7886666777556925 2316 86-87 -1.0298832393466206 2316 88-89 -0.9440225156030095 2316 90-91 -0.8829545593661976 2316 92-93 -0.8564338813365602 2316 94-95 -0.8134337213520944 2316 96-97 -0.7217976040544478 2316 98-99 -0.8572672935946208 2316 100-101 -0.6639845973536005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 16.0 4 15.0 5 47.0 6 78.0 7 179.0 8 441.0 9 790.0 10 1309.0 11 2114.0 12 3187.0 13 4478.0 14 5804.0 15 7379.0 16 9071.0 17 11997.0 18 11443.0 19 8680.0 20 8187.0 21 8662.0 22 9515.0 23 10817.0 24 13234.0 25 16057.0 26 19812.0 27 25371.0 28 31710.0 29 39715.0 30 48887.0 31 61709.0 32 77547.0 33 100026.0 34 138588.0 35 208816.0 36 362357.0 37 661602.0 38 666319.0 39 116397.0 40 81.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.39084399123658 17.488743146362744 12.258211796057951 24.862201066342724 2 16.670177249122446 18.949269668827625 38.28919843929534 26.09135464275458 3 20.787434738540036 21.967294337959004 27.83580240815112 29.409468515349836 4 13.338649455010865 14.516911989463829 34.613671841776174 37.53076671374913 5 14.63966314557173 36.58961261517902 33.538921401747636 15.231802837501613 6 36.22158199312965 33.63875653264568 15.848047068104421 14.291614406120251 7 31.598636032236943 30.406482746684325 18.970606205005943 19.024275016072785 8 28.26708423106336 34.644758897044646 18.323534906454704 18.764621965437286 9 26.74222145794204 15.353402621192162 18.59039331996008 39.313982600905724 10-11 26.741345178890448 25.413752632380415 26.027380470430057 21.817521718299083 12-13 26.745403280634473 22.005774323739853 26.958871639126862 24.289950756498808 14-15 23.16022758547176 23.879668211610365 24.378082474663305 28.58202172825457 16-17 23.95440912931651 27.02110550897425 25.15345831360205 23.87102704810719 18-19 23.94381079757053 25.927848318940562 26.609553642626633 23.51878724086228 20-21 24.275461227133636 25.231472454137272 26.8966553349252 23.596410983803892 22-23 23.806912437860653 25.056319805499594 26.905202778227082 24.231564978412674 24-25 23.801183676342202 25.28780821538757 27.06670264482151 23.844305463448716 26-27 23.62023357807379 25.572412619931246 26.86500696782322 23.942346834171747 28-29 23.61754051102285 25.94787260140593 26.77906888856328 23.655517999007944 30-31 23.696806386854874 25.57035438576292 26.747480412493662 23.985358814888546 32-33 23.53388623563711 25.40677020879339 26.87750346020472 24.181840095364784 34-35 23.921568700277763 25.68020771101709 26.749200386496106 23.649023202209037 36-37 23.96307210959293 25.3957892785958 26.69162299649407 23.949515615317203 38-39 23.718774751084755 25.653846475259822 26.802881780694594 23.824496992960825 40-41 23.971937503040948 25.42067189393627 26.83748331890393 23.769907284118855 42-43 23.847837968488754 25.407336107747142 26.654893762508863 24.089932161255234 44-45 23.822658144418273 25.660994556564575 26.726504651833515 23.789842647183633 46-47 24.039692713555052 25.908804378471523 26.41571706051935 23.63578584745407 48-49 24.09629200521608 25.871270028563615 26.31741511105894 23.71502285516137 50-51 23.873861074540574 26.074686358486126 26.376344559297294 23.67510800767601 52-53 24.220955701959817 25.822149651535653 26.218418039098285 23.73847660740625 54-55 23.991533295771216 25.831860375891065 26.335773385296324 23.840832943041395 56-57 23.870563371911633 26.196905317268122 26.154618995973795 23.777912314846457 58-59 23.930676028319695 25.838656337352294 26.257835939803936 23.97283169452407 60-61 23.690355988882043 25.96546243195898 26.4587581012713 23.885423477887677 62-63 23.76822650392451 25.935852214293437 26.63044548084469 23.665475800937365 64-65 23.70394237143977 25.9880999028753 26.421019790782623 23.886937934902306 66-67 23.601110368702873 26.263993353238458 26.26781888094772 23.867077397110947 68-69 23.847206937650213 25.843315298879567 26.54331258758323 23.766165175886993 70-71 23.70764121575609 25.87746429203491 26.34646415331272 24.068430338896274 72-73 23.794734351658782 26.102791790503172 26.209258377488588 23.893215480349458 74-75 23.620497994569238 26.318534978879743 26.114036381139925 23.946930645411094 76-77 23.929161656836794 26.217458433762513 26.010404072317193 23.842975837083497 78-79 23.810072652872748 26.25398798872265 26.115289077827917 23.82065028057669 80-81 23.914718072389395 26.09809572164131 26.09599723073483 23.891188975234463 82-83 23.935082651826097 26.22792939784337 26.143600653533838 23.693387296796697 84-85 23.807041535805652 26.357419930887556 25.92409293632801 23.91144559697878 86-87 23.897321934080008 26.24334657742569 26.07978027041153 23.77955121808277 88-89 23.774033567138947 26.408652364363167 25.824412294875266 23.99290177362262 90-91 23.728111696152844 26.419123599783767 26.009178362223416 23.843586341839973 92-93 23.81953525483727 26.34338798005548 26.07837115132854 23.758705613778712 94-95 23.77587326596168 26.080062665780464 26.261370754520584 23.882693313737278 96-97 23.946745021482947 25.93820755557933 26.123397693034534 23.99164972990319 98-99 23.483295408096655 26.195137088912933 25.97519981087214 24.346367692118267 100-101 23.589863680106017 26.08878936658029 26.063273259677082 24.258073693636607 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 51.0 1 44.5 2 79.5 3 154.0 4 128.0 5 95.5 6 242.0 7 486.0 8 766.5 9 896.0 10 783.0 11 711.0 12 799.5 13 903.5 14 979.0 15 1113.0 16 1259.5 17 1384.5 18 1530.0 19 1733.0 20 2056.5 21 2524.0 22 3172.5 23 4171.0 24 5299.0 25 6771.5 26 9086.5 27 11990.0 28 14622.5 29 17220.5 30 20316.5 31 24798.5 32 31596.0 33 35480.5 34 37212.5 35 40999.0 36 46289.0 37 51360.0 38 55318.0 39 59381.0 40 63690.0 41 67867.5 42 71431.0 43 75917.0 44 81432.0 45 87382.5 46 92090.5 47 96266.5 48 100861.5 49 103974.0 50 103939.0 51 104417.0 52 108201.5 53 111516.5 54 114035.5 55 109906.5 56 101946.0 57 95808.5 58 90962.0 59 83930.0 60 72182.5 61 61347.0 62 51126.0 63 39260.5 64 28157.5 65 20492.0 66 15079.0 67 11671.5 68 9016.0 69 7052.0 70 5579.0 71 3714.0 72 2489.5 73 1969.5 74 1493.0 75 988.0 76 658.0 77 369.0 78 208.0 79 111.5 80 56.5 81 27.0 82 14.0 83 9.0 84 6.0 85 2.5 86 2.0 87 1.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2668955545510966 2 0.021467524426737244 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.342694114889882E-4 12-13 9.285261430249673E-5 14-15 0.0 16-17 5.199746400939817E-4 18-19 0.0 20-21 7.428209144199738E-5 22-23 9.285261430249673E-5 24-25 0.002395597449004416 26-27 0.0023398858804229176 28-29 0.0020241869917944286 30-31 0.0012627955545139557 32-33 0.0015042123517004472 34-35 0.001652776534584442 36-37 9.285261430249673E-5 38-39 2.414167971864915E-4 40-41 7.428209144199738E-4 42-43 0.003658393003518371 44-45 0.0042155086893333514 46-47 0.013296494368117532 48-49 0.014020744759677005 50-51 0.0057011505181733 52-53 0.007353927052757741 54-55 3.714104572099869E-4 56-57 0.0029341426119588966 58-59 0.0013742186916769516 60-61 0.0013927892145374511 62-63 0.0 64-65 8.913850973039687E-4 66-67 0.0 68-69 0.0 70-71 2.785578429074902E-4 72-73 0.0016713470574449413 74-75 0.0 76-77 0.19395054075505516 78-79 0.10399492801879634 80-81 0.0010399492801879634 82-83 0.0 84-85 5.571156858149804E-4 86-87 0.12314113708797117 88-89 0.5836343924597734 90-91 0.30827067948428916 92-93 0.2931357033529822 94-95 0.002247033266120421 96-97 0.002637014246190907 98-99 0.002989854180540395 100-101 8.171030058619712E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2692439.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.08304393862528 #Duplication Level Percentage of deduplicated Percentage of total 1 72.31182585056827 36.21596351356231 2 14.709738066469797 14.734169158173515 3 5.379968384340742 8.083355789440567 4 2.5071097920315277 5.022547594930908 5 1.346923730842851 3.37290201968898 6 0.8716810762945245 2.619386498671611 7 0.54445253184895 1.9087488052560724 8 0.39694834702114995 1.5904305204179945 9 0.28199887011901026 1.271102562253177 >10 1.4666810329157554 13.437568742986908 >50 0.1176248293986165 4.0367722133984865 >100 0.06031216393526774 5.303293324203177 >500 0.003329524837688908 1.140218171342585 >1k 0.0014057993759007891 1.2635410856736056 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3971 0.1474870925580858 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2830 0.10510915939042631 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.428209144199738E-5 2 0.0 0.0 0.0 0.0 7.428209144199738E-5 3 0.0 0.0 0.0 0.0 7.428209144199738E-5 4 0.0 0.0 0.0 3.714104572099869E-5 7.428209144199738E-5 5 0.0 0.0 0.0 3.714104572099869E-5 1.4856418288399477E-4 6 0.0 0.0 0.0 3.714104572099869E-5 1.8570522860499345E-4 7 0.0 0.0 0.0 3.714104572099869E-5 1.8570522860499345E-4 8 0.0 0.0 0.0 3.714104572099869E-5 1.8570522860499345E-4 9 0.0 0.0 0.0 7.428209144199738E-5 1.8570522860499345E-4 10-11 0.0 0.0 0.0 7.428209144199738E-5 2.5998732004699085E-4 12-13 0.0 0.0 0.0 7.428209144199738E-5 6.499683001174771E-4 14-15 0.0 0.0 0.0 7.428209144199738E-5 7.428209144199738E-4 16-17 1.8570522860499346E-5 0.0 0.0 2.2284627432599217E-4 8.728145744434693E-4 18-19 3.714104572099869E-5 0.0 0.0 2.785578429074902E-4 0.001021378757327464 20-21 3.714104572099869E-5 0.0 0.0 3.7141045720998696E-4 0.0012070839859324575 22-23 3.714104572099869E-5 0.0 0.0 5.942567315359791E-4 0.0012256545087929568 24-25 3.714104572099869E-5 0.0 0.0 8.913850973039687E-4 0.0012256545087929568 26-27 3.714104572099869E-5 0.0 0.0 0.0010585198030484627 0.001392789214537451 28-29 3.714104572099869E-5 0.0 0.0 0.001615635488863443 0.001448500783118949 30-31 3.714104572099869E-5 0.0 0.0 0.0031569888862848887 0.0014670713059794483 32-33 5.571156858149804E-5 0.0 0.0 0.005812573655336295 0.001559923920281945 34-35 7.428209144199738E-5 0.0 0.0 0.009693812933180659 0.001559923920281945 36-37 7.428209144199738E-5 0.0 0.0 0.016899175803054407 0.001559923920281945 38-39 7.428209144199738E-5 0.0 0.0 0.02817148317937751 0.0015784944431424443 40-41 7.428209144199738E-5 0.0 0.0 0.04156083016179754 0.001597064966002944 42-43 7.428209144199738E-5 0.0 0.0 0.054337349889821085 0.0016156354888634432 44-45 7.428209144199738E-5 0.0 0.0 0.06934233236110457 0.0016899175803054406 46-47 7.428209144199738E-5 0.0 0.0 0.08743002162723093 0.0017456291488869386 48-49 7.428209144199738E-5 0.0 0.0 0.10865612925678167 0.0019499049003524314 50-51 7.428209144199738E-5 0.0 0.0 0.1338006172098978 0.0020056164689339294 52-53 7.428209144199738E-5 0.0 0.0 0.1594650798031079 0.002098469083236426 54-55 7.428209144199738E-5 0.0 0.0 0.18563094651355147 0.0021727511746784233 56-57 7.428209144199738E-5 0.0 0.0 0.21825935517944883 0.0022656037889809202 58-59 7.428209144199738E-5 0.0 0.0 0.2559203755405415 0.002358456403283417 60-61 7.428209144199738E-5 0.0 0.0 0.29920826432836545 0.0024513090175859137 62-63 7.428209144199738E-5 0.0 0.0 0.3407505239673025 0.0024884500633069124 64-65 7.428209144199738E-5 0.0 0.0 0.38556119562968744 0.002507020586167412 66-67 1.1142313716299608E-4 0.0 0.0 0.43676012715608414 0.002525591109027911 68-69 1.1142313716299608E-4 0.0 0.0 0.49581438985247206 0.00254416163188841 70-71 1.1142313716299608E-4 0.0 0.0 0.5635782277704342 0.0026555847690514067 72-73 1.1142313716299608E-4 0.0 0.0 0.6335148168630746 0.0028784310433773988 74-75 1.1142313716299608E-4 0.0 0.0 0.7049927593531367 0.0030269952262613935 76-77 1.1142313716299608E-4 0.0 0.0 0.7855702580448434 0.0030455657491218926 78-79 1.1142313716299608E-4 0.0 0.0 0.8820441243051375 0.0031569888862848887 80-81 1.8570522860499345E-4 0.0 0.0 0.9902917020589881 0.0031755594091453882 82-83 1.8570522860499345E-4 0.0 0.0 1.1047604049711062 0.0032312709777268865 84-85 1.8570522860499345E-4 0.0 0.0 1.2174649082114768 0.0032312709777268865 86-87 1.8570522860499345E-4 0.0 0.0 1.3425559501998001 0.003324123592029383 88-89 1.8570522860499345E-4 0.0 0.0 1.4845275974683179 0.0034355467291923795 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5420 0.0 40.553757 1 GGTATCA 3115 0.0 40.474007 1 TCAACGC 6405 0.0 33.774605 4 ATCAACG 6560 0.0 32.976578 3 CAACGCA 6590 0.0 32.826458 5 TATCAAC 6975 0.0 31.157501 2 AACGCAG 7000 0.0 31.039307 6 GTACATG 9320 0.0 26.085154 1 TACATGG 9190 0.0 25.713144 2 ACATGGG 8740 0.0 25.619766 3 ACGCAGA 8520 0.0 25.557459 7 CGCAGAG 8790 0.0 24.772419 8 TAACGCA 205 2.708839E-8 23.141459 4 GTGTAGC 1790 0.0 21.528828 1 GAGTACA 6265 0.0 21.49086 1 GTACCTG 2520 0.0 21.144913 1 TAGACCG 270 1.0186341E-9 21.084438 5 CATGGGG 5940 0.0 20.525383 4 GCGTATC 140 4.8019754E-4 20.389736 1 TACCTGG 2380 0.0 20.335955 2 >>END_MODULE