##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936356_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 827983 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07922264104456 33.0 31.0 34.0 30.0 34.0 2 32.170202769863636 34.0 31.0 34.0 30.0 34.0 3 32.227001037460916 34.0 31.0 34.0 30.0 34.0 4 35.72416583432268 37.0 35.0 37.0 35.0 37.0 5 35.766425156072046 37.0 35.0 37.0 35.0 37.0 6 35.89044944159482 37.0 36.0 37.0 35.0 37.0 7 35.84902950905997 37.0 36.0 37.0 35.0 37.0 8 35.86114811535985 37.0 36.0 37.0 35.0 37.0 9 37.56537755002216 39.0 38.0 39.0 35.0 39.0 10-11 37.51805834177755 39.0 38.0 39.0 35.0 39.0 12-13 37.49425652459048 39.0 38.0 39.0 35.0 39.0 14-15 38.73612924419946 40.0 38.0 41.0 35.0 41.0 16-17 38.748041324519946 40.0 38.0 41.0 35.5 41.0 18-19 38.81651374484742 40.0 38.5 41.0 35.5 41.0 20-21 38.77379789198571 40.0 39.0 41.0 35.0 41.0 22-23 38.62314926755742 40.0 38.0 41.0 35.0 41.0 24-25 38.75814781221354 40.0 39.0 41.0 35.0 41.0 26-27 38.67031327937893 40.0 38.0 41.0 35.0 41.0 28-29 38.565650502486164 40.0 38.0 41.0 35.0 41.0 30-31 38.41969098399363 40.0 38.0 41.0 35.0 41.0 32-33 38.19032214429524 40.0 38.0 41.0 34.0 41.0 34-35 37.91941803152963 40.0 38.0 41.0 34.0 41.0 36-37 37.719618035635996 40.0 37.5 41.0 33.0 41.0 38-39 37.56159063652273 40.0 37.0 41.0 33.0 41.0 40-41 37.46429878391223 40.0 37.0 41.0 33.0 41.0 42-43 37.2694028742136 40.0 37.0 41.0 33.0 41.0 44-45 37.03979127590784 40.0 37.0 41.0 32.5 41.0 46-47 36.68870556521088 40.0 37.0 41.0 31.0 41.0 48-49 36.44724952082349 40.0 37.0 41.0 30.5 41.0 50-51 36.08561649212605 39.5 36.5 40.5 30.0 41.0 52-53 36.17292323634664 39.5 37.0 40.5 30.0 41.0 54-55 36.32531344242575 40.0 37.0 41.0 30.0 41.0 56-57 36.2166173701634 40.0 37.0 41.0 29.5 41.0 58-59 36.078793888280316 40.0 36.5 41.0 29.0 41.0 60-61 35.853608105480426 40.0 36.0 41.0 28.0 41.0 62-63 35.616349611042736 39.0 36.0 41.0 28.0 41.0 64-65 35.3583068734503 39.0 35.0 41.0 28.0 41.0 66-67 35.02522817497461 39.0 35.0 40.5 27.0 41.0 68-69 34.619254260051235 38.0 35.0 40.0 26.0 41.0 70-71 34.19603240646245 37.0 35.0 39.5 26.0 41.0 72-73 33.76460929753389 37.0 34.0 39.0 25.5 41.0 74-75 33.32676516305287 36.0 34.0 39.0 24.5 40.5 76-77 32.92259804851066 36.0 34.0 38.5 23.5 40.0 78-79 32.483931433374835 35.5 34.0 37.0 21.5 39.0 80-81 32.01211196365143 35.0 33.0 37.0 20.0 39.0 82-83 31.613524673815768 35.0 33.0 36.0 18.5 38.5 84-85 31.22785491972661 35.0 33.0 36.0 13.0 37.0 86-87 30.95800940357471 35.0 33.0 35.5 7.5 37.0 88-89 30.594968133403704 35.0 32.0 35.0 2.0 36.5 90-91 30.236931193031744 35.0 32.0 35.0 2.0 36.0 92-93 29.942200504116627 35.0 31.5 35.0 2.0 36.0 94-95 29.603399465931062 34.0 31.0 35.0 2.0 36.0 96-97 29.26289911749396 34.0 31.0 35.0 2.0 35.0 98-99 28.942065235638896 34.0 31.0 35.0 2.0 35.0 100-101 25.93842929142265 31.5 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26556583589611193 1101 2 0.18874750921531813 1101 3 0.27411284741658903 1101 4 0.0427738500307413 1101 5 0.2642045079915434 1101 6 0.22009731188096282 1101 7 0.23795305600616956 1101 8 0.25870910778104417 1101 9 0.2505142121065873 1101 10-11 0.3893885230248131 1101 12-13 0.3488877106086363 1101 14-15 0.5048047234762834 1101 16-17 0.46249198925757184 1101 18-19 0.5006679338577058 1101 20-21 0.5687577150118059 1101 22-23 0.5659525759109698 1101 24-25 0.45999888442795367 1101 26-27 0.3339052031064469 1101 28-29 0.2201244036912655 1101 30-31 0.34511067133421847 1101 32-33 0.37139673480353963 1101 34-35 0.36729124367936095 1101 36-37 0.30401970560537706 1101 38-39 0.30406243907824404 1101 40-41 0.14209757591206795 1101 42-43 0.25429277763608127 1101 44-45 0.4667397031594831 1101 46-47 0.2951645372391809 1101 48-49 0.2620766804482315 1101 50-51 0.24087941675247038 1101 52-53 0.3897004808648177 1101 54-55 0.49359064122278795 1101 56-57 0.4385410909795766 1101 58-59 0.6898414240356416 1101 60-61 0.5108403214438795 1101 62-63 0.316866448983113 1101 64-65 0.4067685224454607 1101 66-67 0.5373902730200797 1101 68-69 0.5920643361653575 1101 70-71 0.6520387706495967 1101 72-73 0.6223586314190399 1101 74-75 0.5994098677603148 1101 76-77 0.502069134521129 1101 78-79 0.6418561698107652 1101 80-81 0.7438904281432954 1101 82-83 0.9236896543381263 1101 84-85 0.6575318494438775 1101 86-87 0.5494305882895709 1101 88-89 0.5234545046219203 1101 90-91 0.6011857119417279 1101 92-93 0.567142875581716 1101 94-95 0.7357296630375387 1101 96-97 0.7767629753442478 1101 98-99 1.010140890101706 1101 100-101 1.118398469291428 1102 1 0.17830049614007493 1102 2 0.1727974064316129 1102 3 0.11186361555495239 1102 4 0.0434225489073512 1102 5 0.10281217228110506 1102 6 0.08429570854279689 1102 7 -0.016608655857574206 1102 8 0.11254147171864304 1102 9 0.05554732643099669 1102 10-11 0.06359266154832 1102 12-13 0.06508414364143533 1102 14-15 0.245875251946714 1102 16-17 0.15811962733620533 1102 18-19 0.18936041114228885 1102 20-21 0.19670480222235653 1102 22-23 0.20952322491666564 1102 24-25 0.2201994551076396 1102 26-27 0.19626817642650707 1102 28-29 0.16832273022348687 1102 30-31 0.4245850499201467 1102 32-33 0.1547543212930691 1102 34-35 0.337071957160461 1102 36-37 0.410582741165463 1102 38-39 0.4751578635596161 1102 40-41 0.49686901913558046 1102 42-43 0.7140241374053531 1102 44-45 0.7527720767399089 1102 46-47 0.7747151817741127 1102 48-49 0.6625507127692174 1102 50-51 0.505436789346696 1102 52-53 0.4742482982999121 1102 54-55 0.6328001052134269 1102 56-57 0.7193981186237934 1102 58-59 0.7301133515917613 1102 60-61 0.7273194644600665 1102 62-63 0.782968645354778 1102 64-65 0.6249669366396944 1102 66-67 0.6375397367477262 1102 68-69 0.8968607630618166 1102 70-71 0.9730219877577113 1102 72-73 0.779579351211801 1102 74-75 0.8154200190211185 1102 76-77 0.6543402380543384 1102 78-79 0.751190420673133 1102 80-81 0.5667507080480974 1102 82-83 0.7284915601102284 1102 84-85 0.8021741665261324 1102 86-87 0.8170846213995127 1102 88-89 0.6765145662483114 1102 90-91 0.8086805946718734 1102 92-93 0.9534583974555773 1102 94-95 0.885436652767936 1102 96-97 0.8563315905912994 1102 98-99 0.7962653087930001 1102 100-101 0.8501198443577245 1103 1 0.2208979355426024 1103 2 0.2918668559698361 1103 3 0.31459008506450914 1103 4 0.18542234886067632 1103 5 0.11731555640406555 1103 6 0.13936689181889506 1103 7 0.20148806671102903 1103 8 0.15388445174731658 1103 9 0.18556516392641242 1103 10-11 0.2054111692001115 1103 12-13 0.31561343380914053 1103 14-15 0.30122149940445553 1103 16-17 0.36424480654468283 1103 18-19 0.37952770017639637 1103 20-21 0.5084712977379766 1103 22-23 0.40030107308123064 1103 24-25 0.44636055936529573 1103 26-27 0.3954021410182449 1103 28-29 0.30135168698006254 1103 30-31 0.43509166813105793 1103 32-33 0.5102518540507148 1103 34-35 0.21109881342692205 1103 36-37 0.2685225104449671 1103 38-39 0.2656068849979505 1103 40-41 0.35883056016182024 1103 42-43 0.34110795696324914 1103 44-45 0.3419720567352371 1103 46-47 0.38430325232390317 1103 48-49 0.3909706774276387 1103 50-51 0.3661563739164322 1103 52-53 0.41826648587701243 1103 54-55 0.30822020323630284 1103 56-57 0.42266221357927947 1103 58-59 0.4618382540690007 1103 60-61 0.3515505350432022 1103 62-63 0.4997546454214685 1103 64-65 0.3843899686805088 1103 66-67 0.5312920289492382 1103 68-69 0.4447573556000677 1103 70-71 0.4966108251531054 1103 72-73 0.4178470303369366 1103 74-75 0.6737640492948458 1103 76-77 0.41916869802833645 1103 78-79 0.36929297793650306 1103 80-81 0.35635578256548683 1103 82-83 0.33016111634002243 1103 84-85 0.3136122520794338 1103 86-87 0.220564183297288 1103 88-89 0.16358238207200415 1103 90-91 0.3374664780446608 1103 92-93 0.29060581518638173 1103 94-95 0.3222990039832183 1103 96-97 0.2503006000268364 1103 98-99 0.2895533259970158 1103 100-101 1.0222808485920432 1104 1 0.12708982190162033 1104 2 0.11434579832110359 1104 3 0.10301931730028713 1104 4 0.03982374905757524 1104 5 -0.030791208609763032 1104 6 -0.12979986600642945 1104 7 -0.13611604949281286 1104 8 -0.09422856414553848 1104 9 0.06234666444992598 1104 10-11 0.046842972109892855 1104 12-13 0.05955594134926656 1104 14-15 0.21309191868216715 1104 16-17 0.06410201302684015 1104 18-19 -0.002116788892386978 1104 20-21 0.04955227006221463 1104 22-23 0.11848019201983817 1104 24-25 0.08412602638409794 1104 26-27 0.001141620007480526 1104 28-29 0.06685679707366887 1104 30-31 0.20961660132478954 1104 32-33 0.18813917414260573 1104 34-35 0.16096188010818224 1104 36-37 -0.018690730479541173 1104 38-39 0.044735004577262316 1104 40-41 0.1516617786144181 1104 42-43 0.14716466806324746 1104 44-45 0.17953319814575508 1104 46-47 0.07814105764028056 1104 48-49 0.22052874028022273 1104 50-51 0.15205693024466171 1104 52-53 0.15285318556915684 1104 54-55 0.12769286345415054 1104 56-57 0.18587500912865096 1104 58-59 0.13698202428668793 1104 60-61 0.12948122060835487 1104 62-63 0.2047102251123576 1104 64-65 0.25458460542630235 1104 66-67 0.12152053764577886 1104 68-69 0.16082777956594185 1104 70-71 0.18709073639062268 1104 72-73 0.18628810017488462 1104 74-75 -0.07134366116443402 1104 76-77 0.06280608453581493 1104 78-79 0.16942502775600587 1104 80-81 0.12496191467781159 1104 82-83 -0.09384968205350575 1104 84-85 -0.24906746069267527 1104 86-87 0.023584334568560905 1104 88-89 -0.017956818753109616 1104 90-91 0.03175356529130369 1104 92-93 -0.16063635168083223 1104 94-95 -0.3374763941196406 1104 96-97 0.07327277580155567 1104 98-99 0.06943155105991394 1104 100-101 0.4799799781571359 1105 1 0.0554606772374342 1105 2 0.0675968885510585 1105 3 0.13667604468815142 1105 4 -0.03250645964089216 1105 5 0.01961755695216283 1105 6 0.08655942554198504 1105 7 0.10157104225988434 1105 8 0.04621416156127367 1105 9 -0.016031163834618667 1105 10-11 0.24813786753450273 1105 12-13 0.04301591850813935 1105 14-15 0.0421246387729326 1105 16-17 0.07110323046600087 1105 18-19 0.0921753814284827 1105 20-21 0.022499113577936214 1105 22-23 0.0800611291087634 1105 24-25 0.05580454706359461 1105 26-27 0.16218375546360164 1105 28-29 -0.06453051819030264 1105 30-31 0.09149296529010087 1105 32-33 0.07895598912577384 1105 34-35 -0.054146992121019366 1105 36-37 0.028846876329076565 1105 38-39 0.10717116440478947 1105 40-41 0.10412417180580036 1105 42-43 0.1780077164102849 1105 44-45 0.05981332222655311 1105 46-47 0.13132298375717255 1105 48-49 0.21641752740175235 1105 50-51 0.15663740372672663 1105 52-53 0.2584453019910171 1105 54-55 0.3093278126270036 1105 56-57 0.41046709162805683 1105 58-59 0.4591548704935917 1105 60-61 0.48088391405114805 1105 62-63 0.3993751655030664 1105 64-65 0.3259150440436187 1105 66-67 0.3372795902293504 1105 68-69 0.32178416659851905 1105 70-71 0.33116976063010384 1105 72-73 0.5033556171643809 1105 74-75 0.4295628221702117 1105 76-77 0.4395243510230671 1105 78-79 0.49543864950558003 1105 80-81 0.4823377867675518 1105 82-83 0.37643330659000895 1105 84-85 0.47558775526143293 1105 86-87 0.4591740764186909 1105 88-89 0.3886706863258347 1105 90-91 0.2997202157505754 1105 92-93 0.19158896125379954 1105 94-95 0.16930998111546103 1105 96-97 0.054818961221684503 1105 98-99 0.16383585393465339 1105 100-101 0.42553820981734347 1106 1 0.05493382776121791 1106 2 0.0957725098545481 1106 3 0.008397218624253355 1106 4 -0.022629836240838586 1106 5 0.08249307252489757 1106 6 -0.012374606408194211 1106 7 -0.13953476427088418 1106 8 -0.2298585725349085 1106 9 -0.010396400429726782 1106 10-11 -0.10977064830164807 1106 12-13 0.004674087020589468 1106 14-15 0.014510148247197208 1106 16-17 0.06230376164575091 1106 18-19 -0.09730188285147534 1106 20-21 0.010174083536689693 1106 22-23 0.025460037159092508 1106 24-25 0.09104031220420694 1106 26-27 0.13784854207152364 1106 28-29 0.07527765086342697 1106 30-31 0.24019983851062676 1106 32-33 0.21771587206414722 1106 34-35 0.24821318527569503 1106 36-37 0.26010583006375754 1106 38-39 0.17953718766555227 1106 40-41 0.04263885322425409 1106 42-43 0.05741692328530945 1106 44-45 0.17705898203334414 1106 46-47 0.47445084579478447 1106 48-49 0.5108370727956029 1106 50-51 0.43015437657954436 1106 52-53 0.3358136888573142 1106 54-55 0.3834214330572152 1106 56-57 0.3131415744314765 1106 58-59 0.2608122812088496 1106 60-61 0.33280492993240784 1106 62-63 0.3970647862075296 1106 64-65 0.21397486671907018 1106 66-67 0.27327369384548916 1106 68-69 0.3796690463794121 1106 70-71 0.3309739963555529 1106 72-73 0.3737831230373061 1106 74-75 0.2555473270219224 1106 76-77 0.414553129273564 1106 78-79 0.5292706855622882 1106 80-81 0.5417247288393696 1106 82-83 0.5038938921146467 1106 84-85 0.6492405903250678 1106 86-87 0.6155590590434841 1106 88-89 0.48238764546612956 1106 90-91 0.360389098071888 1106 92-93 0.36302704381039064 1106 94-95 0.41336578385724465 1106 96-97 0.5610220725204407 1106 98-99 0.4234824317586181 1106 100-101 0.6919291826485079 1107 1 -0.017256539730652776 1107 2 -0.010108990548545194 1107 3 -0.03987945556072958 1107 4 -0.021334511186715588 1107 5 0.008036339099170675 1107 6 -0.02899281161304401 1107 7 -0.11473336568215586 1107 8 -0.03290756843153986 1107 9 -0.09386424828790751 1107 10-11 -0.10153582307994924 1107 12-13 0.10260609604257098 1107 14-15 0.004156606031372689 1107 16-17 -0.14838131131818955 1107 18-19 -0.08543924165651617 1107 20-21 -0.06852461298586121 1107 22-23 0.11789642582908044 1107 24-25 0.08119584179287642 1107 26-27 0.013500054340873646 1107 28-29 0.08340377747918382 1107 30-31 0.11503701705715486 1107 32-33 0.08998414022360635 1107 34-35 0.004856934652941902 1107 36-37 0.10367404296808758 1107 38-39 0.06734482736590053 1107 40-41 0.13201809360216288 1107 42-43 -0.02453639066343527 1107 44-45 0.14901745916587572 1107 46-47 0.14183401743893853 1107 48-49 -0.11789196875443508 1107 50-51 0.039030523576293774 1107 52-53 0.009590150174190626 1107 54-55 0.16072154084999823 1107 56-57 -0.11645343267922925 1107 58-59 0.1543265977210737 1107 60-61 0.14798197278683745 1107 62-63 0.1488995280936365 1107 64-65 0.055606432794242266 1107 66-67 0.042586265745086394 1107 68-69 -0.03315195419126127 1107 70-71 0.1003943610393705 1107 72-73 -0.12982838814506437 1107 74-75 0.011306088582472285 1107 76-77 0.04352855433040048 1107 78-79 0.1638087617781281 1107 80-81 -0.012460620400197797 1107 82-83 -0.02928816899208897 1107 84-85 -0.02857130673735142 1107 86-87 -0.03657007490037145 1107 88-89 -0.019821653699608532 1107 90-91 0.2758387932628814 1107 92-93 0.0014529068320285887 1107 94-95 0.21448447367671264 1107 96-97 0.22687802875113405 1107 98-99 0.40779359963455875 1107 100-101 0.4005064084789183 1108 1 -0.1425883745922576 1108 2 0.002491794446306983 1108 3 0.07779655101211347 1108 4 -0.026204544306345667 1108 5 0.053928738612164295 1108 6 -0.014072801055625916 1108 7 0.00854147616948353 1108 8 -0.1350863122695003 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-0.934059915772437 2316 62-63 -1.01080119983326 2316 64-65 -0.6437466534490142 2316 66-67 -0.7236556776172023 2316 68-69 -0.8664144010263826 2316 70-71 -0.8016536035054642 2316 72-73 -0.8000131695882899 2316 74-75 -0.6922543198190709 2316 76-77 -0.7906298025324787 2316 78-79 -0.9388348876255925 2316 80-81 -0.9086607466853849 2316 82-83 -0.948600272117055 2316 84-85 -0.9329084881948724 2316 86-87 -1.081757536925462 2316 88-89 -1.1387036723564599 2316 90-91 -1.215355793153119 2316 92-93 -1.1407227725465496 2316 94-95 -1.1354517880433868 2316 96-97 -1.4325480884558175 2316 98-99 -1.3492444169731357 2316 100-101 -1.4191952725437496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4060.0 3 581.0 4 447.0 5 376.0 6 472.0 7 540.0 8 650.0 9 816.0 10 1139.0 11 1566.0 12 2568.0 13 4362.0 14 4908.0 15 4730.0 16 6228.0 17 8905.0 18 7664.0 19 3691.0 20 3153.0 21 2959.0 22 3101.0 23 3434.0 24 3698.0 25 4247.0 26 4932.0 27 5889.0 28 7150.0 29 8510.0 30 10808.0 31 13721.0 32 17766.0 33 23779.0 34 33112.0 35 53922.0 36 80137.0 37 163778.0 38 267401.0 39 62760.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.456332500374586 23.034128752543587 14.431372738565345 21.07816600851648 2 13.837243012103496 24.36752591017805 40.32263909356654 21.472591984151908 3 17.732365716604903 30.42375911368369 31.726732022670063 20.117143147041343 4 9.907358198859793 19.083727896415112 44.46383708570876 26.545076819016327 5 8.986910144185298 42.76301406099581 37.28984301111375 10.96023278370514 6 24.443668742541586 43.73915963300979 19.110691319493093 12.706480304955528 7 24.09491140555361 36.55961252762915 24.770330825069752 14.575145241747492 8 23.520388258044722 35.42480218093183 22.6776566820026 18.37715287902085 9 25.73440680320591 18.08237049206071 24.634925620134204 31.54829708459917 10-11 21.447128292894437 29.033874947612016 30.928840507417654 18.590156252075893 12-13 23.401700802086232 28.555139578465365 30.0989185333798 17.944241086068605 14-15 20.78962101259386 30.060810227278907 29.313923663808644 19.835645096318594 16-17 17.34239335598339 33.13008682521706 30.364424311060777 19.16309550773877 18-19 16.928714178468518 32.99600239892302 33.299551923215134 16.775731499393324 20-21 18.361088565520706 31.3295939861248 32.511184223239994 17.798133225114498 22-23 18.30062556846526 30.824315395700136 32.36897064454449 18.506088391290117 24-25 18.799457593539444 31.30174530452982 32.587937194405605 17.310859907525135 26-27 17.78787822054785 30.945523437731193 34.08766569654907 17.178932645171887 28-29 17.177528366776716 31.275752974389263 34.137013373728735 17.409705285105286 30-31 17.817782751891514 30.92379398713401 33.38181282286525 17.87661043810923 32-33 17.205476539770988 32.1421474805175 33.44895293096586 17.203423048745652 34-35 18.252115128893884 30.903892127820875 33.40565423062111 17.43833851266413 36-37 18.39967232512629 29.997239035786343 33.11542954231709 18.48765909677028 38-39 17.013477409517012 31.911017908189056 33.52787016153353 17.547634520760404 40-41 16.80027772163868 31.689094995244865 34.26105469890325 17.249572584213198 42-43 17.124998103560753 31.609727974754602 32.20099221701334 19.06428170467131 44-45 17.625547773097512 31.036975442770608 32.475630925357194 18.861845858774686 46-47 18.56662636210011 31.231790105471706 32.43540343414331 17.76618009828487 48-49 18.05470571095571 31.58520299145299 32.265503593628594 18.094587703962702 50-51 17.124424097421258 31.336081840848422 32.715698243743766 18.823795817986554 52-53 17.603353193090868 32.424114581594466 31.8319639914167 18.140568233897966 54-55 18.082659876972194 31.07578015228257 31.765209289904377 19.07635068084086 56-57 18.893456850364263 31.461217642931878 30.837745522871774 18.80757998383209 58-59 18.783064769167932 30.480247470281537 31.815006618264224 18.92168114228631 60-61 18.58952573535659 32.11846056371876 31.358039825898242 17.9339738750264 62-63 17.97423876928679 32.03046440004248 32.04684963512509 17.948447195545643 64-65 17.821800206853354 31.821365620584334 31.827860136057062 18.528974036505247 66-67 18.19575261320236 31.779004331368498 30.61169505903598 19.413547996393156 68-69 17.662354531444517 31.403302294781067 31.445183399786707 19.48915977398771 70-71 18.83154555142088 31.02875743695429 31.878166243084937 18.261530768539895 72-73 18.654556930527825 30.360014808431096 32.02626675810671 18.95916150293438 74-75 17.757648669844052 32.35428012304883 30.737261119433608 19.15081008767351 76-77 17.6620351545004 30.968903282033477 32.01800329914827 19.351058264317857 78-79 17.235019293724655 31.14986714701709 32.089366541926076 19.525747017332186 80-81 18.612195304026848 31.31178812223586 30.846099363524303 19.229917210212985 82-83 18.655118941768755 30.579095745165098 31.811817401094284 18.953967911971866 84-85 17.366995740610587 30.713365791604307 31.7614013621792 20.15823710560591 86-87 17.91276861505519 31.249901372769664 31.427248306342854 19.4100817058323 88-89 18.05311056019464 30.540453915243575 31.424658665157985 19.981776859403798 90-91 17.87842872478397 29.531660602998965 32.365170422254714 20.224740249962355 92-93 19.214930847934667 30.779635580363134 31.425471734195742 18.57996183750646 94-95 18.3963784295887 29.21629949450228 32.34475320798965 20.042568867919375 96-97 18.147828134139445 29.29840614146435 32.27709159043636 20.27667413395984 98-99 18.026341893730862 30.290629631809274 31.326152783954363 20.3568756905055 100-101 17.73595209057648 29.448277117425413 31.936217849536742 20.879552942461366 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1769.0 1 1084.5 2 740.5 3 1057.0 4 720.0 5 721.0 6 1746.5 7 2834.5 8 3407.0 9 3180.0 10 2301.0 11 1799.5 12 1814.5 13 1905.0 14 1760.5 15 1513.5 16 1550.0 17 1601.0 18 1552.5 19 1586.0 20 1713.0 21 1983.0 22 2413.0 23 2960.5 24 3384.0 25 4292.0 26 7264.5 27 10484.0 28 10563.5 29 11377.5 30 16305.5 31 20543.0 32 26918.0 33 35976.0 34 42556.5 35 48587.0 36 57012.0 37 61169.5 38 55268.5 39 48116.0 40 41126.0 41 34081.5 42 27214.5 43 25805.5 44 27873.0 45 25296.5 46 20212.5 47 20972.0 48 31910.5 49 29729.5 50 16298.5 51 8378.0 52 4244.0 53 2804.0 54 1783.5 55 1373.0 56 1150.0 57 910.5 58 802.5 59 662.5 60 540.5 61 468.5 62 374.5 63 277.0 64 204.0 65 141.0 66 106.0 67 97.0 68 80.0 69 57.5 70 54.0 71 43.5 72 41.5 73 39.0 74 32.5 75 29.0 76 16.5 77 9.0 78 10.0 79 9.0 80 5.0 81 3.0 82 4.0 83 3.0 84 3.0 85 5.0 86 4.0 87 3.0 88 4.5 89 5.5 90 4.0 91 3.0 92 3.5 93 3.5 94 4.0 95 6.0 96 6.0 97 3.0 98 2.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04963870031147983 2 0.014855377465479363 3 0.012922970640701562 4 0.0076088518725626 5 0.010869788389375143 6 0.00712575016636815 7 0.006401097607076473 8 0.009662034123889017 9 0.016667008863708553 10-11 0.0031401610902639307 12-13 0.00960164641061471 14-15 0.00960164641061471 16-17 0.020592210226538468 18-19 0.01346646006017032 20-21 0.0037440382230069943 22-23 0.011594440948666821 24-25 0.02300771875751072 26-27 0.009239320130968872 28-29 0.02004872080706971 30-31 0.017089722856628697 32-33 0.015036540605302282 34-35 0.2735563411326078 36-37 0.4825582167749821 38-39 0.4800219328174612 40-41 0.4995875519183365 42-43 0.49161637376612805 44-45 0.506411363518333 46-47 0.5138994399643471 48-49 0.5196966604386806 50-51 0.5295398577023924 52-53 0.5181869676068229 54-55 0.5055055478192185 56-57 0.4986817362192219 58-59 0.4983797976528504 60-61 0.4948169225696663 62-63 0.49161637376612805 64-65 0.5085853211962081 66-67 0.48237705363515915 68-69 0.5099742386015172 70-71 0.48998590550772175 72-73 0.49856096079267326 74-75 0.512691685698861 76-77 0.499285613351965 78-79 0.5000102659112566 80-81 0.5011576324634684 82-83 0.5018822850227601 84-85 0.5019426727360344 86-87 0.5041166304139094 88-89 0.5199382112917778 90-91 0.5433686440422086 92-93 0.5313514891006216 94-95 0.5001914290510796 96-97 0.47645905773427716 98-99 0.4660119833378221 100-101 0.47440587548295077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 827983.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.644602138964705 #Duplication Level Percentage of deduplicated Percentage of total 1 71.79665227409058 11.232300597377426 2 9.951976893546506 3.1138943799140995 3 4.063032317776102 1.9069357226808816 4 2.3752533829597704 1.4863957662254228 5 1.5292187443274092 1.196200941922475 6 1.1606584590545208 1.0894823886679101 7 0.8545892927368295 0.9358796633960853 8 0.6377501659585145 0.7981878088383737 9 0.5536654696789292 0.7795688392088886 >10 5.012955716905082 16.870505348818057 >50 0.9700497131008377 10.675947318227209 >100 0.965423084898449 30.08330604021555 >500 0.0940747734485709 9.877576533318933 >1k 0.03315750211711925 8.236923363969803 >5k 0.0015422094007962442 1.716895287218885 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7578 0.9152361823853872 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6654 0.803639688254469 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4221 0.5097930754616943 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3448 0.4164336707396166 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3269 0.39481486938741495 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3259 0.3936071151219288 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2506 0.30266321893082343 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2141 0.2585801882405798 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2006 0.2422755056565171 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1937 0.23394200122466283 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1835 0.22162290771670431 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1789 0.21606723809546816 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1688 0.20386892001405826 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1591 0.19215370363884285 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1574 0.19010052138751643 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1557 0.18804733913619 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1535 0.1853902797521205 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 1534 0.1852695043255719 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1513 0.18273322036805104 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1390 0.16787784290257168 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1387 0.16751551662292583 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1382 0.16691163949018276 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1353 0.163409152120273 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1348 0.16280527498752992 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1333 0.16099364358930074 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1280 0.15459254598222427 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1225 0.14794989752205057 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1224 0.14782912209550195 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 1217 0.14698369410966167 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1201 0.14505128728488387 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1179 0.1423942279008144 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1173 0.14166957534152272 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1166 0.1408241473556824 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1163 0.14046182107603658 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1161 0.14022027022293934 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1140 0.1376839862654185 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1131 0.13659700742648098 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1097 0.13249064292382814 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1073 0.12959203268666145 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1070 0.12922970640701562 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 1063 0.1283842784211753 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1061 0.1281427275680781 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1029 0.12427791391852248 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1020 0.12319093507958497 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 1010 0.12198318081409884 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 982 0.11860146887073769 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 979 0.11823914259109183 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 957 0.11558208320702236 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 949 0.11461587979463346 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 945 0.11413277808843901 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 945 0.11413277808843901 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 943 0.1138912272353418 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 914 0.11038873986543203 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 907 0.10954331187959174 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 900 0.10869788389375144 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 884 0.10676547706897363 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 871 0.10519539652384167 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 866 0.10459151939109862 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 860 0.10386686683180693 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 858 0.10362531597870972 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 845 0.10205523543357774 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 839 0.10133058287428608 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 836 0.10096825659464022 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 833 0.10060593031499439 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.0387713274306355E-5 0.0 12-13 0.0 0.0 0.0 1.2077542654861271E-4 0.0 14-15 0.0 0.0 0.0 4.8310170619445084E-4 0.0 16-17 0.0 0.0 0.0 0.0012077542654861271 0.0 18-19 0.0 0.0 0.0 0.001872019111503497 0.0 20-21 0.0 0.0 0.0 0.003260936516812543 0.0 22-23 0.0 0.0 0.0 0.006521873033625086 0.0 24-25 0.0 0.0 0.0 0.011171726955746676 0.0 26-27 0.0 0.0 0.0 0.014855377465479363 0.0 28-29 0.0 0.0 0.0 0.02089414879291 0.0 30-31 0.0 0.0 0.0 0.03943317676812205 0.0 32-33 0.0 0.0 0.0 0.07783976241058088 0.0 34-35 0.0 0.0 0.0 0.13345684633621704 0.0 36-37 0.0 0.0 0.0 0.21190048587954102 0.0 38-39 0.0 0.0 0.0 0.3189679015148862 0.0 40-41 0.0 0.0 0.0 0.43817324751836695 0.0 42-43 0.0 0.0 0.0 0.5570162672422019 0.0 44-45 0.0 0.0 0.0 0.69385482552178 0.0 46-47 0.0 0.0 0.0 0.8673487257588621 0.0 48-49 0.0 0.0 0.0 1.0443451133658541 0.0 50-51 0.0 0.0 0.0 1.2181409521693078 0.0 52-53 0.0 0.0 0.0 1.3744243541232126 0.0 54-55 0.0 0.0 0.0 1.5543797396806456 0.0 56-57 0.0 0.0 0.0 1.7699638760699195 0.0 58-59 0.0 0.0 0.0 1.9918283346397208 0.0 60-61 0.0 0.0 0.0 2.2331980245971232 0.0 62-63 0.0 0.0 0.0 2.462610947326213 0.0 64-65 0.0 0.0 0.0 2.6939562768800807 0.0 66-67 0.0 0.0 0.0 2.9722228596480846 0.0 68-69 0.0 0.0 0.0 3.2858162546815572 0.0 70-71 0.0 0.0 0.0 3.6293619554990864 0.0 72-73 0.0 0.0 0.0 3.953885526635209 0.0 74-75 0.0 0.0 0.0 4.290728191279289 0.0 76-77 0.0 0.0 0.0 4.660542547371142 0.0 78-79 0.0 0.0 0.0 5.115201640613394 0.0 80-81 0.0 0.0 0.0 5.598544897660942 0.0 82-83 0.0 0.0 0.0 6.045474363604084 0.0 84-85 0.0 0.0 0.0 6.51631736400385 0.0 86-87 0.0 0.0 0.0 7.016991894761125 0.0 88-89 0.0 1.2077542654861271E-4 0.0 7.579141117631642 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 260 0.0 47.326935 9 CCTTTAG 285 0.0 41.522373 1 GGCATTC 290 0.0 40.796616 7 CCCCGAC 30 8.210691E-5 39.649845 84-85 CCCGACA 25 0.0016027451 38.063854 86-87 ACTCCCG 200 0.0 37.85926 6 GCTTTGC 90 5.852253E-7 36.816505 1 GTATTGA 65 1.3552401E-4 36.41193 1 TAGGCAT 325 0.0 36.405334 5 CTTTAGG 335 0.0 35.32074 2 CATGGGG 1205 0.0 34.56241 4 TGGGTAT 180 0.0 34.178497 6 TACTCCC 225 0.0 33.654705 5 CTCCCGC 225 0.0 33.652676 7 GTGGTAC 240 0.0 33.52932 1 GACTGGT 100 1.3368099E-6 33.12685 7 AGGCATT 360 0.0 32.86394 6 TTAGGCA 395 0.0 32.350056 4 TAGCGTT 30 0.003919102 31.719877 86-87 CGGGTGT 30 0.003920267 31.71795 54-55 >>END_MODULE