##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936356_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 827983 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.43897036533359 34.0 31.0 34.0 31.0 34.0 2 32.56889694595179 34.0 31.0 34.0 31.0 34.0 3 32.6553371264869 34.0 31.0 34.0 31.0 34.0 4 36.14366116212531 37.0 35.0 37.0 35.0 37.0 5 36.132253923087795 37.0 35.0 37.0 35.0 37.0 6 36.19059086961931 37.0 37.0 37.0 35.0 37.0 7 36.14635686964588 37.0 36.0 37.0 35.0 37.0 8 36.13193386820744 37.0 36.0 37.0 35.0 37.0 9 37.87734289230576 39.0 38.0 39.0 35.0 39.0 10-11 37.825980726657434 39.0 38.0 39.0 35.0 39.0 12-13 37.820977000735525 39.0 38.0 39.0 35.0 39.0 14-15 39.0743451254434 40.5 38.0 41.0 36.0 41.0 16-17 39.043852953502665 40.0 38.0 41.0 36.0 41.0 18-19 39.17034709649836 40.5 38.5 41.0 36.0 41.0 20-21 39.14853143120088 40.0 39.0 41.0 36.0 41.0 22-23 39.02139536681308 40.0 38.5 41.0 35.0 41.0 24-25 39.13800766440857 40.5 39.0 41.0 35.0 41.0 26-27 39.004091871451465 40.5 38.5 41.0 35.0 41.0 28-29 38.922857715677736 40.0 38.0 41.0 35.0 41.0 30-31 38.78869433309622 40.0 38.0 41.0 35.0 41.0 32-33 38.56729184053296 40.0 38.0 41.0 35.0 41.0 34-35 38.39540304571471 40.0 38.0 41.0 34.0 41.0 36-37 38.229322341159175 40.0 38.0 41.0 34.0 41.0 38-39 38.078983505699995 40.0 38.0 41.0 34.0 41.0 40-41 38.00091064671618 40.0 38.0 41.0 34.0 41.0 42-43 37.93647876828389 40.0 38.0 41.0 33.5 41.0 44-45 37.71895316691285 40.0 38.0 41.0 33.0 41.0 46-47 37.41602967693781 40.0 38.0 41.0 33.0 41.0 48-49 37.11514910330284 40.0 38.0 41.0 32.5 41.0 50-51 36.921784022135725 40.0 37.0 41.0 31.5 41.0 52-53 36.81635009414445 40.0 37.0 41.0 31.0 41.0 54-55 36.699145393081736 40.0 37.0 41.0 31.0 41.0 56-57 36.5055218525018 40.0 37.0 41.0 31.0 41.0 58-59 36.32971993386337 40.0 36.0 41.0 30.0 41.0 60-61 36.10710485601757 39.5 36.0 41.0 29.5 41.0 62-63 35.872906207011496 39.0 36.0 41.0 29.0 41.0 64-65 35.564169795756676 39.0 35.0 40.5 28.0 41.0 66-67 35.23166840381988 38.5 35.0 40.0 28.0 41.0 68-69 34.8481134274496 37.5 35.0 40.0 28.0 41.0 70-71 34.46093398052859 37.0 35.0 39.5 26.5 41.0 72-73 34.031576131394004 37.0 34.0 39.0 26.0 41.0 74-75 33.5795940254812 36.0 34.0 39.0 26.0 40.0 76-77 32.79022516162772 35.0 33.0 37.0 26.0 39.0 78-79 32.77683479008627 35.0 34.0 37.0 26.0 39.0 80-81 32.52698545743089 35.0 34.0 37.0 26.0 39.0 82-83 32.17982917523669 35.0 33.5 36.0 25.0 38.5 84-85 31.826887750110814 35.0 33.0 36.0 24.5 37.0 86-87 31.46552042735177 35.0 33.0 35.5 22.0 37.0 88-89 30.936043372871183 35.0 32.5 35.0 18.0 36.5 90-91 30.701657521953955 35.0 32.0 35.0 15.0 36.0 92-93 30.43816298160711 35.0 32.0 35.0 10.0 36.0 94-95 30.292941400970793 35.0 32.0 35.0 4.5 36.0 96-97 30.082727543923003 34.0 32.0 35.0 2.0 35.0 98-99 29.781954460417666 34.0 32.0 35.0 2.0 35.0 100-101 26.798779685090153 31.5 25.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17958114874430464 1101 2 0.08399838761017264 1101 3 0.19475566539320255 1101 4 0.13336909558099563 1101 5 0.07259791079732736 1101 6 0.15318789587329462 1101 7 0.18610811073948952 1101 8 0.02684385080546292 1101 9 0.23791248845880375 1101 10-11 0.14898508222851348 1101 12-13 0.14759440833126547 1101 14-15 0.25337943193630963 1101 16-17 0.2115435232805325 1101 18-19 0.3548688414121486 1101 20-21 0.20183387948402753 1101 22-23 0.25645524289061683 1101 24-25 0.2020760241884858 1101 26-27 0.24387749536843728 1101 28-29 0.14185282686653267 1101 30-31 0.18865131447405048 1101 32-33 0.0052953685993273325 1101 34-35 0.17599421332105436 1101 36-37 0.17346415762811773 1101 38-39 0.07324307558447885 1101 40-41 0.1863136582913114 1101 42-43 0.18120686868167724 1101 44-45 -0.048512206619093945 1101 46-47 -0.06943573770156064 1101 48-49 -0.018558149610832686 1101 50-51 -0.1950233737180227 1101 52-53 -0.13655666535759536 1101 54-55 0.04104629941408433 1101 56-57 -0.10336751641292352 1101 58-59 -0.16606698687767363 1101 60-61 -0.06933561393501719 1101 62-63 -0.3205384545001735 1101 64-65 -0.11797164720306341 1101 66-67 -0.13409520913900508 1101 68-69 -0.04366144493010182 1101 70-71 -0.14589563101140612 1101 72-73 -0.036276862092499584 1101 74-75 -0.10332142134532063 1101 76-77 -0.009824562680826432 1101 78-79 -0.10718339131285859 1101 80-81 0.005812601780213811 1101 82-83 -0.04972756495773467 1101 84-85 0.13717316439561955 1101 86-87 -0.040671205210422556 1101 88-89 0.011618601396254036 1101 90-91 0.15398620473206392 1101 92-93 0.1419620360736893 1101 94-95 0.33471364934592174 1101 96-97 0.19443000233397356 1101 98-99 0.38344581744032524 1101 100-101 0.8698450147256978 1102 1 0.05787121357035829 1102 2 0.14937014028057405 1102 3 0.1897983787077422 1102 4 0.06644791687759977 1102 5 0.045486242545443645 1102 6 0.006476755346710661 1102 7 -0.002165780445658072 1102 8 -0.027237034931488324 1102 9 0.038338964139171594 1102 10-11 -0.06307589546589298 1102 12-13 0.10387845954340946 1102 14-15 0.023667708457352887 1102 16-17 0.10975587573441459 1102 18-19 0.32181478463346735 1102 20-21 0.06711380619858431 1102 22-23 0.18073060679753894 1102 24-25 0.21791326213386242 1102 26-27 0.26824205537089796 1102 28-29 0.16247093170153448 1102 30-31 0.14137738384099663 1102 32-33 0.1632031393982416 1102 34-35 0.2879177808142259 1102 36-37 0.36774727962296083 1102 38-39 0.3621617208557737 1102 40-41 0.1778696151440684 1102 42-43 0.41803893895154687 1102 44-45 0.16193250275942717 1102 46-47 0.34506531205101254 1102 48-49 0.4259923415155811 1102 50-51 0.2879329399191164 1102 52-53 0.21413520705910827 1102 54-55 0.08190556243956593 1102 56-57 0.3269455684570559 1102 58-59 0.31884857269084677 1102 60-61 0.3326505880645598 1102 62-63 0.4101320722170172 1102 64-65 0.26213302123728965 1102 66-67 0.37573718109720033 1102 68-69 0.11696275241461507 1102 70-71 0.068932178884765 1102 72-73 0.17485599406729335 1102 74-75 0.09219992654574583 1102 76-77 0.06918739880048719 1102 78-79 0.25693391798940013 1102 80-81 0.19351888144832685 1102 82-83 0.24227678307797618 1102 84-85 0.24369346472566633 1102 86-87 0.33200191630227494 1102 88-89 0.4148254968899927 1102 90-91 0.5879493165581096 1102 92-93 0.6934623713808783 1102 94-95 0.5355703263420963 1102 96-97 0.4181993372861328 1102 98-99 0.50962980246765 1102 100-101 1.0235954146836193 1103 1 0.09818478673743414 1103 2 0.1682120367230766 1103 3 0.1947286957817269 1103 4 0.08358941656086216 1103 5 0.21518417206232954 1103 6 0.21213724279378 1103 7 0.22982585093499353 1103 8 0.2004244194078595 1103 9 0.20745342417984602 1103 10-11 0.23139073008007216 1103 12-13 0.18914835585254508 1103 14-15 0.10351967391596162 1103 16-17 0.3001065408896224 1103 18-19 0.2501147212853496 1103 20-21 0.3163219547665861 1103 22-23 0.22173809187738414 1103 24-25 0.25867694032543653 1103 26-27 0.27652732193309504 1103 28-29 0.2814216035249615 1103 30-31 0.3675801644898158 1103 32-33 0.3683364204971653 1103 34-35 0.26364545060384614 1103 36-37 0.4755995284810268 1103 38-39 0.4994645915255944 1103 40-41 0.5536802209520957 1103 42-43 0.5291148566656787 1103 44-45 0.5156775432688789 1103 46-47 0.4080550096471214 1103 48-49 0.20150454436291199 1103 50-51 0.1626828815721737 1103 52-53 0.15683225303650516 1103 54-55 0.2698056557946842 1103 56-57 0.2732309517131526 1103 58-59 0.22688336414218924 1103 60-61 0.19103212709033102 1103 62-63 0.17313302243874062 1103 64-65 0.256012843195748 1103 66-67 0.14284950730665003 1103 68-69 0.01292806098661714 1103 70-71 -0.27524396221482306 1103 72-73 0.07813134164839397 1103 74-75 0.30779814946442485 1103 76-77 0.20628397133408072 1103 78-79 -0.021053862492713904 1103 80-81 0.07112573971525649 1103 82-83 -0.05739730754316952 1103 84-85 -0.06147565806965005 1103 86-87 -0.1258111567481066 1103 88-89 0.01365480706236255 1103 90-91 0.03264266167196794 1103 92-93 0.1366282767588025 1103 94-95 0.04997160330671946 1103 96-97 -0.15866859856556204 1103 98-99 -0.07333330559089646 1103 100-101 0.11962407137017905 1104 1 -0.02145508449446254 1104 2 -0.0020208866567728023 1104 3 -0.028085840637459114 1104 4 0.0018923810402426966 1104 5 0.06122892835618643 1104 6 -0.03684503596416988 1104 7 -0.09267848561233194 1104 8 0.07038660158972476 1104 9 0.01145320539907857 1104 10-11 -0.01998934680575104 1104 12-13 -0.003999899370846549 1104 14-15 0.1352428849925147 1104 16-17 0.15725735115981365 1104 18-19 0.20938633760639647 1104 20-21 0.09295851303259184 1104 22-23 0.182098728529418 1104 24-25 0.07837619490811676 1104 26-27 0.046514507631087554 1104 28-29 0.18072287245340135 1104 30-31 0.1066997281784623 1104 32-33 -0.005160438436128345 1104 34-35 0.1417650299196893 1104 36-37 0.05210735770700836 1104 38-39 0.1459324541937832 1104 40-41 0.11562936223759124 1104 42-43 0.07516039431941834 1104 44-45 0.19243872843554044 1104 46-47 0.20562419906438123 1104 48-49 0.27799479299162755 1104 50-51 0.3548016951553308 1104 52-53 0.2464356738300566 1104 54-55 0.040979531808496006 1104 56-57 0.1748880206759722 1104 58-59 0.19601476543299867 1104 60-61 0.08345742908591802 1104 62-63 -0.036264588162751465 1104 64-65 0.21281047880075477 1104 66-67 0.08735342191964435 1104 68-69 0.050705834423503404 1104 70-71 0.02431964829290223 1104 72-73 -0.01158928312695906 1104 74-75 0.1095583579312418 1104 76-77 0.03529801237110064 1104 78-79 0.024512115535443968 1104 80-81 0.03405119016922242 1104 82-83 0.16327239600889243 1104 84-85 0.3490986805092149 1104 86-87 0.22839197337956918 1104 88-89 0.40499792562590997 1104 90-91 0.44066781364646346 1104 92-93 0.2769312099837862 1104 94-95 0.39760154345455234 1104 96-97 0.3627367553123264 1104 98-99 0.19266406122630997 1104 100-101 0.1364809340539921 1105 1 0.05491189614335923 1105 2 0.11123676191414944 1105 3 0.09575561335427096 1105 4 -0.010534544341666674 1105 5 -0.07886138075274829 1105 6 -0.11335147557654324 1105 7 0.0134309482219237 1105 8 -0.04249601541446424 1105 9 0.1377615755759649 1105 10-11 0.08808853160864061 1105 12-13 0.1445851574115764 1105 14-15 0.1288843095847909 1105 16-17 0.1902686096451376 1105 18-19 0.08995668712073979 1105 20-21 0.11935018167806533 1105 22-23 -0.058656460663641496 1105 24-25 0.1663342893708446 1105 26-27 0.2584553996408374 1105 28-29 0.18715871567136588 1105 30-31 0.2986876216318066 1105 32-33 0.30984817409784426 1105 34-35 0.35909016909597824 1105 36-37 0.31314063563367256 1105 38-39 0.35458852197838553 1105 40-41 0.41132196450436 1105 42-43 0.5372982250162224 1105 44-45 0.5455601595107709 1105 46-47 0.4624991495941373 1105 48-49 0.5529193155330958 1105 50-51 0.44239041179353933 1105 52-53 0.6803603885987641 1105 54-55 0.6391096508009255 1105 56-57 0.523149118029643 1105 58-59 0.6517657520110092 1105 60-61 0.8115312744975327 1105 62-63 0.7640197950705101 1105 64-65 0.7670223282906008 1105 66-67 0.8432088142705112 1105 68-69 0.7935547928157334 1105 70-71 0.5616786345392271 1105 72-73 0.5481783103765068 1105 74-75 0.6192780830839908 1105 76-77 0.7130761670650259 1105 78-79 0.661642690596409 1105 80-81 0.8148207398674998 1105 82-83 0.8744066874630718 1105 84-85 0.7042573433614905 1105 86-87 0.630474812010462 1105 88-89 0.7185073541833411 1105 90-91 0.751791362990442 1105 92-93 0.7436400912589711 1105 94-95 0.75837154545221 1105 96-97 0.7156348635701804 1105 98-99 0.7915630891595669 1105 100-101 1.133488219843123 1106 1 -0.1193958922074927 1106 2 -0.03561170390739221 1106 3 -0.0603488038816451 1106 4 0.03739071520386972 1106 5 -0.08842225532288239 1106 6 -0.025966633685520435 1106 7 -0.04986676864339756 1106 8 0.036758745175745844 1106 9 -0.040414587011227354 1106 10-11 0.06809441355827772 1106 12-13 0.04057117785766451 1106 14-15 0.08564837123339686 1106 16-17 -0.038895576137868204 1106 18-19 0.06263809835562029 1106 20-21 0.1664581028238601 1106 22-23 0.07573706702662975 1106 24-25 -0.040222280189532 1106 26-27 -0.00676910679863596 1106 28-29 -0.02829746019532564 1106 30-31 -0.03689925620768264 1106 32-33 -0.08538726627254078 1106 34-35 0.01851536188318903 1106 36-37 0.14391910986314826 1106 38-39 0.15042623068836036 1106 40-41 -0.1178784872871077 1106 42-43 0.010218703222328429 1106 44-45 0.09567761807549857 1106 46-47 -0.02467513293431267 1106 48-49 0.042071990015898564 1106 50-51 0.01073379496105531 1106 52-53 -0.09976475619463088 1106 54-55 -0.06898326235190666 1106 56-57 -0.12768860872312615 1106 58-59 -0.22006097224824117 1106 60-61 -0.26949709237734254 1106 62-63 -0.28743734067261784 1106 64-65 -0.10758569209053803 1106 66-67 -0.2192706620555498 1106 68-69 -0.2135648922293214 1106 70-71 -0.004640347558712676 1106 72-73 -0.17807106263652628 1106 74-75 -0.02667816675813839 1106 76-77 -0.1222526988734316 1106 78-79 -0.35561433616825866 1106 80-81 -0.24409348109894324 1106 82-83 -0.2141789021211835 1106 84-85 0.06491106973896876 1106 86-87 0.03858107682810186 1106 88-89 -0.1194719976474623 1106 90-91 0.10900432926124992 1106 92-93 0.3429692603163055 1106 94-95 0.3198767111030669 1106 96-97 0.3254910986172206 1106 98-99 0.3210612469906806 1106 100-101 0.7329364713163216 1107 1 -6.293931928524898E-5 1107 2 0.08557999514240322 1107 3 0.06903695841262447 1107 4 0.06628642794968442 1107 5 0.011305092340556655 1107 6 0.07347447431560283 1107 7 0.07817294258064322 1107 8 0.0613699212040828 1107 9 0.13665544206565272 1107 10-11 -0.06852614678303581 1107 12-13 0.0691109026690313 1107 14-15 0.10004187830333677 1107 16-17 0.11452888286011387 1107 18-19 0.07148978816603346 1107 20-21 0.15601850754500646 1107 22-23 0.004630968129013979 1107 24-25 0.11608540933102063 1107 26-27 0.3550070284224347 1107 28-29 0.20012813107980065 1107 30-31 0.006126369488661965 1107 32-33 0.18242435904336674 1107 34-35 0.13254019951465068 1107 36-37 0.24220982428474258 1107 38-39 0.36761600939611583 1107 40-41 0.17592569217428888 1107 42-43 0.11851331267730103 1107 44-45 0.26613424280262166 1107 46-47 0.12572297570994806 1107 48-49 -0.007840598188487036 1107 50-51 0.03752618968192678 1107 52-53 0.20528157658615243 1107 54-55 0.2293590839075037 1107 56-57 0.047517239261878785 1107 58-59 0.02698559604618822 1107 60-61 0.2932745409097848 1107 62-63 0.2303131416774491 1107 64-65 0.27497542822976584 1107 66-67 0.07186043752143689 1107 68-69 0.4306600414192374 1107 70-71 0.3717693374870734 1107 72-73 0.19451424312359222 1107 74-75 0.33349466402530936 1107 76-77 -0.018321432625263867 1107 78-79 0.10003935007321019 1107 80-81 0.2487541506301767 1107 82-83 0.45955407975332463 1107 84-85 0.2756803841225093 1107 86-87 0.1348219167059952 1107 88-89 0.2564422029195832 1107 90-91 0.21000163897075197 1107 92-93 0.6320975880788353 1107 94-95 0.4708183127770269 1107 96-97 0.04608948444292338 1107 98-99 0.06268194320525211 1107 100-101 0.39732318472338335 1108 1 -0.19214828409549511 1108 2 -0.10097803232390845 1108 3 -0.01441088881137631 1108 4 0.022728579278300742 1108 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-1.727619331687734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 15.0 6 34.0 7 77.0 8 189.0 9 353.0 10 616.0 11 1126.0 12 1894.0 13 2830.0 14 3490.0 15 4649.0 16 6246.0 17 9158.0 18 7920.0 19 3805.0 20 2869.0 21 2808.0 22 2852.0 23 3171.0 24 3680.0 25 4152.0 26 4877.0 27 5993.0 28 7702.0 29 9718.0 30 12137.0 31 14923.0 32 19038.0 33 24162.0 34 32848.0 35 51200.0 36 75054.0 37 157483.0 38 284555.0 39 66346.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.54778759454271 23.522772001429114 14.847793044562998 21.081647359465173 2 13.777549057980467 24.598210187050913 40.0036965629213 21.620544192047316 3 17.562196325286873 30.29397946576198 32.04667245583545 20.097151753115703 4 9.894768370848194 19.376484782900132 44.00996155718173 26.718785289069945 5 8.83556788001686 42.81706266916108 37.395574546820406 10.951794904001652 6 24.264507846175587 43.92131239409505 18.936741454836632 12.877438304892733 7 23.771985656710342 36.63782952065441 24.657027982458576 14.93315684017667 8 23.360745329312316 35.610272191578815 22.578845217836598 18.450137261272275 9 25.19302932548132 18.00459671273444 24.56777494223915 32.23459901954509 10-11 21.210282126209496 29.188851669815897 30.816063904886647 18.78480229908796 12-13 23.083640052778893 28.63424045798069 30.16054083268668 18.12157865655373 14-15 20.483089628651797 30.17664613887 29.406763182335872 19.933501050142333 16-17 17.168874052209116 33.155953419052196 30.382147834845853 19.293024693892832 18-19 16.72039160224304 32.88322344782441 33.4130048563799 16.983380093552643 20-21 18.09294393725475 31.281197802370336 32.655682544206826 17.970175716168086 22-23 18.070660871056532 30.730220306455568 32.555680491024575 18.643438331463326 24-25 18.492421465438998 31.405430311843457 32.69648963994186 17.40565858277569 26-27 17.524987514546233 30.95957732436273 34.265520158799276 17.249915002291768 28-29 16.879276297911144 31.322036559516402 34.306401840657514 17.492285301914936 30-31 17.569825604321878 30.801710441215814 33.62275483454483 18.005709119917483 32-33 16.994305942798547 32.11505027063228 33.478145605780895 17.41249818078828 34-35 17.94804747770897 30.892577305268627 33.57976007512372 17.579615141898685 36-37 18.173995223328994 29.96820584976132 33.29708055423877 18.56071837267092 38-39 16.7890427371176 31.857090837284215 33.704598115535326 17.64926831006286 40-41 16.578972953942532 31.65874876883583 34.38732862587284 17.3749496513488 42-43 16.98638144867181 31.497376355982205 32.321277272883734 19.194964922462248 44-45 17.391821602646054 30.97157495716828 32.668840309704876 18.967763130480783 46-47 18.33082589607916 31.207900269426197 32.577256950211414 17.88401688428323 48-49 17.86640076147773 31.609298145589133 32.31629832919417 18.208002763738975 50-51 16.912077320359643 31.264161791064964 32.85112787040482 18.972633018170573 52-53 17.406026888973976 32.414185860279275 31.886057036705107 18.293730214041645 54-55 17.930186550850387 31.029689194640437 31.878322853988873 19.161801400520304 56-57 18.663771186184793 31.4108703712874 30.974736610717706 18.950621831810103 58-59 18.544199340435437 30.67035600906071 31.716830490941405 19.068614159562447 60-61 18.416609297438313 32.18305011051125 31.315023491189294 18.085317100861143 62-63 17.761596554518633 32.07982531042304 32.101081785495595 18.057496349562733 64-65 17.70100685346366 31.912115757048365 31.646286635292952 18.740590754195022 66-67 18.049404395983977 31.826076139244403 30.479128194661005 19.64539127011062 68-69 17.478498954688686 31.53977799061092 31.30142768631723 19.680295368383167 70-71 18.797498739405725 31.092021872442956 31.716068878267357 18.394410509883965 72-73 18.502573752315893 30.445521236174084 31.845806752385947 19.20609825912408 74-75 17.54504621471697 32.403261902720224 30.6032249454397 19.448466937123104 76-77 17.41550019756305 31.102776955859028 31.74102963929768 19.740693207280245 78-79 17.092925826916172 31.182148259114307 31.770000205542637 19.954925708426885 80-81 18.37702143129323 31.398604903671202 30.535652495285166 19.68872116975041 82-83 18.439509023735994 30.672489652565332 31.479873379042804 19.408127944655867 84-85 17.166972734129534 30.83602010443533 31.330720135172918 20.66628702626222 86-87 17.563833582238452 31.384839562007606 31.075270117479185 19.976056738274757 88-89 17.807222314350295 30.709141149721887 30.923566704874506 20.560069831053315 90-91 17.620133972868615 29.411818156485836 32.01803751191859 20.950010358726956 92-93 18.815814894917228 30.929661982391423 30.964426116222434 19.290097006468915 94-95 18.0275845160028 29.2735980830096 31.86253758464282 20.83627981634478 96-97 17.666465167034335 29.48384490285443 31.75364465731072 21.09604527280051 98-99 17.46492720330114 30.389640035436642 30.854761089501682 21.290671671760535 100-101 17.053703689165843 29.599258917954995 31.597378434343405 21.749658958535758 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1767.0 1 1078.5 2 707.5 3 1007.0 4 714.5 5 705.5 6 1667.0 7 2794.0 8 3207.0 9 2815.0 10 2024.5 11 1546.5 12 1466.5 13 1381.5 14 1244.5 15 1163.5 16 1182.0 17 1277.0 18 1390.0 19 1507.5 20 1763.0 21 2133.0 22 2500.5 23 2998.0 24 3428.5 25 4416.5 26 7523.0 27 10772.5 28 10736.0 29 11649.0 30 16575.0 31 20744.5 32 27042.5 33 35900.5 34 42687.5 35 48835.5 36 56733.0 37 60566.5 38 54915.0 39 47809.5 40 41154.0 41 34279.5 42 27271.5 43 25867.5 44 27748.0 45 25431.5 46 20478.5 47 21440.5 48 32359.0 49 29102.5 50 16059.5 51 9453.0 52 5381.5 53 3400.0 54 1972.5 55 1412.0 56 1103.5 57 820.5 58 724.5 59 604.5 60 514.0 61 458.0 62 342.5 63 244.0 64 191.5 65 112.5 66 83.5 67 92.5 68 75.0 69 52.0 70 51.0 71 45.5 72 42.0 73 42.0 74 30.0 75 21.5 76 12.0 77 5.0 78 2.5 79 4.0 80 3.0 81 1.0 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.277541930208712 2 0.022585004764590577 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.019385663715318E-4 12-13 6.0387713274306355E-5 14-15 0.0 16-17 6.038771327430636E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002355120817697948 26-27 0.002355120817697948 28-29 0.002173957677875029 30-31 0.0011473665522118207 32-33 0.0017512436849548842 34-35 0.0018720191115034971 36-37 6.0387713274306355E-5 38-39 3.019385663715318E-4 40-41 7.850402725659827E-4 42-43 0.0038044259362813 44-45 0.004045976789378526 46-47 0.01358723548671893 48-49 0.014372275759284913 50-51 0.006159546753979248 52-53 0.007246525592916764 54-55 6.038771327430636E-4 56-57 0.0031401610902639307 58-59 0.001509692831857659 60-61 0.0015700805451319653 62-63 0.0 64-65 9.058156991145955E-4 66-67 0.0 68-69 0.0 70-71 6.0387713274306355E-5 72-73 0.0010869788389375145 74-75 0.0 76-77 0.20103069749016583 78-79 0.10918098559994588 80-81 0.0013889174053090462 82-83 0.0 84-85 7.850402725659827E-4 86-87 0.12415713849197388 88-89 0.5861835327536918 90-91 0.31304990561400414 92-93 0.29221614453436845 94-95 0.002234345391149335 96-97 0.0029589979504410116 98-99 0.0032005488035382365 100-101 5.434894194687572E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 827983.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.482061631884491 #Duplication Level Percentage of deduplicated Percentage of total 1 69.76373815924256 10.103227556928026 2 10.39979529086913 3.012209527226977 3 4.423312369846201 1.921760470715692 4 2.5275232864211823 1.4641499203989918 5 1.6502625391258792 1.1949601900205586 6 1.2475130902619869 1.0839936875854064 7 0.9388646356934667 0.9517686862676695 8 0.6920404079417077 0.8017737471653037 9 0.6069908761552627 0.7911431350624873 >10 5.451235598281289 16.903168606050677 >50 1.1047547927688808 11.254933036907545 >100 1.0580632694518564 30.71590887167194 >500 0.09921948704867685 9.858480933358187 >1k 0.035018642487768294 8.237799555594288 >5k 0.0016675544041794429 1.7047220750462475 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7660 0.9251397673623735 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6458 0.7799677046509409 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4443 0.5366052201554863 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3497 0.42235166664049867 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3464 0.4183660775643945 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3252 0.39276168713608856 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2508 0.3029047697839207 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2168 0.26184112475739235 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2095 0.25302451861934366 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1919 0.2317680435467878 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1807 0.21824119577334317 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1802 0.2176373186406001 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1704 0.20580132683883606 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1691 0.2042312462937041 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1676 0.2024196148954749 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1655 0.19988333093795405 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1524 0.18406175006008577 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1518 0.18333709750079408 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 1512 0.18261244494150242 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1495 0.180559262690176 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1408 0.1700518005804467 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1404 0.16956869887425224 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1401 0.1692063725946064 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1359 0.16413380467956468 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1357 0.16389225382646744 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1267 0.1530224654370923 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1243 0.1501238551999256 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1215 0.14674214325656446 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1204 0.1454136135645297 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1203 0.1452928381379811 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1194 0.1442058592990436 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1190 0.1437227575928491 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 1183 0.14287732960700883 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1155 0.13949561766364768 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1146 0.13840863882471016 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1113 0.13442304974860597 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1096 0.13236986749727955 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1078 0.1301959098194045 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1067 0.12886738012736978 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 1052 0.12705574872914058 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1052 0.12705574872914058 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1043 0.12596876989020306 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1041 0.12572721903710585 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1022 0.12343248593268219 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 976 0.117876816311446 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 973 0.11751449003180017 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 969 0.11703138832560571 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 967 0.11678983747250848 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 962 0.11618596033976542 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 962 0.11618596033976542 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 948 0.11449510436808485 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 942 0.11377045180879317 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 940 0.11352890095569594 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 930 0.11232114669020982 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 921 0.1112341678512723 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 915 0.11050951529198064 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 904 0.10918098559994588 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 902 0.10893943474684867 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 900 0.10869788389375144 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 899 0.10857710846720282 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 898 0.10845633304065422 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 896 0.108214782187557 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 872 0.10531617195039028 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 866 0.10459151939109862 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 864 0.10434996853800138 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 836 0.10096825659464022 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 836 0.10096825659464022 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.0387713274306355E-5 0.0 12-13 0.0 0.0 0.0 1.2077542654861271E-4 0.0 14-15 0.0 0.0 0.0 4.8310170619445084E-4 0.0 16-17 0.0 0.0 0.0 0.0012077542654861271 0.0 18-19 0.0 0.0 0.0 0.0017512436849548845 0.0 20-21 0.0 0.0 0.0 0.002898610237166705 0.0 22-23 0.0 0.0 0.0 0.006038771327430636 0.0 24-25 0.0 0.0 0.0 0.010567849823003612 0.0 26-27 0.0 0.0 0.0 0.014311888046010607 0.0 28-29 0.0 0.0 0.0 0.020471434799989856 0.0 30-31 0.0 0.0 0.0 0.03937278905484774 0.0 32-33 0.0 0.0 0.0 0.07814170097695242 0.0 34-35 0.0 0.0 0.0 0.1341211111822344 0.0 36-37 0.0 0.0 0.0 0.21334979099812434 0.0 38-39 0.0 0.0 0.0 0.322289225744973 0.0 40-41 0.0 0.0 0.0 0.4436081417130545 0.0 42-43 0.0 0.0 0.0 0.5636589157023755 0.0 44-45 0.0 0.0 0.0 0.7026110439465545 0.0 46-47 0.0 0.0 0.0 0.8787620035677061 0.0 48-49 0.0 0.0 0.0 1.0575096348596529 0.0 50-51 0.0 0.0 0.0 1.2334794313409816 0.0 52-53 0.0 0.0 0.0 1.3927218312453276 0.0 54-55 0.0 0.0 0.0 1.5739453587815209 0.0 56-57 0.0 0.0 0.0 1.7900729845902634 0.0 58-59 0.0 0.0 0.0 2.0134471359919224 0.0 60-61 0.0 0.0 0.0 2.2562661310679086 0.0 62-63 0.0 0.0 0.0 2.4875510729085017 0.0 64-65 0.0 0.0 0.0 2.72016454444113 0.0 66-67 0.0 0.0 0.0 2.9982499640693105 0.0 68-69 0.0 0.0 0.0 3.311541420536412 0.0 70-71 0.0 0.0 0.0 3.6572006913185415 0.0 72-73 0.0 0.0 0.0 3.9840793832723618 0.0 74-75 0.0 0.0 0.0 4.322794067027946 0.0 76-77 0.0 0.0 0.0 4.694721993084399 0.0 78-79 0.0 0.0 0.0 5.147931781208069 0.0 80-81 0.0 0.0 0.0 5.629765345423759 0.0 82-83 0.0 0.0 0.0 6.074520853689025 0.0 84-85 0.0 0.0 0.0 6.543914548970209 0.0 86-87 0.0 0.0 0.0 7.0404827152248295 0.0 88-89 0.0 0.0 0.0 7.602511162668799 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTTCG 20 0.0020841416 71.161316 7 GGCTTAG 45 9.5595524E-4 42.279457 1 GTATGGT 100 2.993147E-8 38.051514 1 GCTTAGG 50 0.0016249873 37.954994 2 TAGACCC 195 0.0 36.492985 5 CATGGGT 485 0.0 36.192013 4 CTTTGCG 55 0.002594242 34.504543 2 TACCTGG 210 0.0 33.88839 2 TGGTACT 115 1.0471922E-7 33.00235 7 GTATAGT 305 0.0 32.749252 1 ATTGCCG 60 0.0039744754 31.627253 8 TCTAGAC 230 0.0 30.939703 3 GGACAAT 375 0.0 30.362162 6 GTACATG 5320 0.0 29.414822 1 GTAACCC 65 0.0058788084 29.194386 3 ATATTGC 65 0.0058788084 29.194386 6 CATGGGG 1350 0.0 29.167353 4 CATTCCG 310 0.0 29.076668 9 TAGGACA 555 0.0 29.06288 4 TGGGTAT 230 0.0 28.877056 6 >>END_MODULE