FastQCFastQC Report
Thu 26 May 2016
SRR936355_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936355_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337475
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48000.35888521280771607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32340.24179891212919866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22580.1688255855249631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30300.038.0014231
GTATCAA48900.035.8532941
TACCTGG13550.034.2655752
GTACCTG14400.032.9058951
GTACATG66950.031.9199491
TACATGG66900.031.301662
ACATGGG67050.029.958643
ATCAACG58600.029.4278663
TCAACGC59050.029.2838344
CAACGCA60050.028.7172855
TATCAAC61900.028.0131322
AACGCAG62100.027.769296
CATGGGG30300.025.3295444
CATGGGA36600.025.1116734
GTCTACG1151.3032707E-424.7223421
AGTACAT45450.023.871122
GAGTACA51900.023.8291931
CGCAGAG74400.023.1155878
ACGCAGA74800.022.9277787
ATGGGAG17550.022.405575
Produced by FastQC (version 0.11.5)