##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936355_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1337475 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38573655582347 34.0 31.0 34.0 31.0 34.0 2 32.49157703882315 34.0 31.0 34.0 31.0 34.0 3 32.56573094824202 34.0 31.0 34.0 31.0 34.0 4 36.04463784369801 37.0 35.0 37.0 35.0 37.0 5 36.05725340660573 37.0 37.0 37.0 35.0 37.0 6 36.156773771472366 37.0 37.0 37.0 35.0 37.0 7 36.12336903493523 37.0 37.0 37.0 35.0 37.0 8 36.13403689788594 37.0 37.0 37.0 35.0 37.0 9 37.88779379053814 39.0 38.0 39.0 35.0 39.0 10-11 37.83240210097385 39.0 38.0 39.0 35.0 39.0 12-13 37.761880782818366 39.0 38.0 39.0 35.0 39.0 14-15 39.12379894951307 40.0 39.0 41.0 36.0 41.0 16-17 39.084461765640484 40.0 39.0 41.0 36.0 41.0 18-19 39.044981775360284 40.0 39.0 41.0 36.0 41.0 20-21 38.97788631563206 40.0 39.0 41.0 36.0 41.0 22-23 38.82610254397278 40.0 38.5 41.0 35.5 41.0 24-25 38.99413559131946 40.0 39.0 41.0 36.0 41.0 26-27 38.958419783547356 40.0 39.0 41.0 36.0 41.0 28-29 38.83970952728089 40.0 39.0 41.0 35.0 41.0 30-31 38.69138937176396 40.0 38.0 41.0 35.0 41.0 32-33 38.51742537243687 40.0 38.0 41.0 35.0 41.0 34-35 38.27906577693041 40.0 38.0 41.0 34.5 41.0 36-37 38.0990852165461 40.0 38.0 41.0 34.0 41.0 38-39 37.94171143385858 40.0 38.0 41.0 34.0 41.0 40-41 37.76026467784445 40.0 38.0 41.0 33.0 41.0 42-43 37.56303743995215 40.0 37.5 41.0 33.0 41.0 44-45 37.36489168021832 40.0 37.0 41.0 33.0 41.0 46-47 37.05198713994654 40.0 37.0 41.0 32.0 41.0 48-49 36.870345613936706 40.0 37.0 41.0 31.5 41.0 50-51 36.563199312136675 39.5 36.5 40.5 31.5 41.0 52-53 36.60148002766407 39.0 36.0 40.5 32.0 41.0 54-55 36.685886839006336 40.0 36.0 41.0 32.0 41.0 56-57 36.52342324155592 39.0 36.0 41.0 31.0 41.0 58-59 36.28927269668592 39.0 35.0 41.0 31.0 41.0 60-61 36.000490850296266 39.0 35.0 41.0 31.0 41.0 62-63 35.7014837660517 38.0 35.0 40.0 30.5 41.0 64-65 35.36956242172751 37.5 35.0 40.0 30.0 41.0 66-67 35.0133408101086 37.0 35.0 40.0 29.0 41.0 68-69 34.634238396979384 36.5 34.5 39.0 29.0 41.0 70-71 34.23725116357315 36.0 34.0 39.0 29.0 41.0 72-73 33.83364997476588 35.5 34.0 38.5 28.0 40.0 74-75 33.43228546327968 35.0 34.0 37.0 27.5 39.5 76-77 33.04813286229649 35.0 34.0 37.0 27.0 39.0 78-79 32.66669208770257 35.0 33.0 36.5 26.0 39.0 80-81 32.290386736200674 35.0 33.0 36.0 26.0 37.5 82-83 31.95889979251949 35.0 33.0 35.5 25.0 37.0 84-85 31.663187349296248 35.0 33.0 35.0 25.0 37.0 86-87 31.37143273706051 35.0 32.0 35.0 24.0 36.0 88-89 31.088122020972357 35.0 32.0 35.0 23.0 36.0 90-91 30.769597562571263 34.0 32.0 35.0 20.0 36.0 92-93 30.48203742126021 34.0 31.0 35.0 18.5 35.0 94-95 30.145471504140264 34.0 31.0 35.0 15.0 35.0 96-97 29.767561262827343 34.0 31.0 35.0 4.5 35.0 98-99 29.312273874278024 34.0 31.0 35.0 2.0 35.0 100-101 25.946138432494067 30.5 24.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20989705194523367 1101 2 0.11315357947314197 1101 3 0.2557417903450059 1101 4 0.11792086633431609 1101 5 0.14183984772333957 1101 6 0.14792217952971498 1101 7 0.17119477129141103 1101 8 0.2266646939289032 1101 9 0.17729658651076363 1101 10-11 0.2882737159586881 1101 12-13 0.3014953769771509 1101 14-15 0.29705221345027866 1101 16-17 0.3416369656037901 1101 18-19 0.39004516417966784 1101 20-21 0.3708179364900488 1101 22-23 0.3897621001580873 1101 24-25 0.37006926146772656 1101 26-27 0.38008411378424256 1101 28-29 0.36363509850887965 1101 30-31 0.28170577992830914 1101 32-33 0.3996563036024554 1101 34-35 0.27323893973429847 1101 36-37 0.1921250504995129 1101 38-39 0.15308172094000838 1101 40-41 -0.037856647350444916 1101 42-43 6.689153688483884E-4 1101 44-45 0.05261175916927385 1101 46-47 0.21177533105093715 1101 48-49 0.25027205816493137 1101 50-51 0.37111178217725893 1101 52-53 0.2561867860157818 1101 54-55 0.3307543024502735 1101 56-57 0.1934370972505448 1101 58-59 0.05460842558679957 1101 60-61 0.009565980798505791 1101 62-63 -0.00531525169929381 1101 64-65 0.12267886876802692 1101 66-67 0.12761299090846023 1101 68-69 0.04559356288694261 1101 70-71 0.11891107061276784 1101 72-73 0.12265295547209121 1101 74-75 0.11390513184059614 1101 76-77 -0.09050048961500323 1101 78-79 0.04812985958880489 1101 80-81 0.16299417433288 1101 82-83 0.20645495537327463 1101 84-85 -0.03059019336185642 1101 86-87 -0.024358005704005592 1101 88-89 -0.2125661407376036 1101 90-91 -0.03824613015953915 1101 92-93 -0.18249367145306294 1101 94-95 -0.022193705190755253 1101 96-97 -0.024935351813912376 1101 98-99 -0.0763586267611025 1101 100-101 0.19592098728131546 1102 1 0.204771749399157 1102 2 0.18350875449643667 1102 3 0.21244908835054588 1102 4 0.1503963343035153 1102 5 0.1952105393733774 1102 6 0.1807134837143849 1102 7 0.16945735939720663 1102 8 0.11179912758140631 1102 9 0.19645877622513552 1102 10-11 0.29400004544152836 1102 12-13 0.3203017037081466 1102 14-15 0.40243018496872907 1102 16-17 0.3545021316836099 1102 18-19 0.3901949518501695 1102 20-21 0.40474030957705054 1102 22-23 0.39211492360876576 1102 24-25 0.3964322041856647 1102 26-27 0.35705746471267474 1102 28-29 0.3285056682023608 1102 30-31 0.30399037024807996 1102 32-33 0.2177496535302197 1102 34-35 0.17905658028141858 1102 36-37 0.24347876829828863 1102 38-39 0.30383097200165565 1102 40-41 0.22831354146332927 1102 42-43 0.25955704680674785 1102 44-45 0.26355313871685127 1102 46-47 0.31151140455008175 1102 48-49 0.41117153468985634 1102 50-51 0.4249321495479421 1102 52-53 0.32158458329271156 1102 54-55 0.38972686535171874 1102 56-57 0.24623865829449443 1102 58-59 0.42524128631240643 1102 60-61 0.3102089107579147 1102 62-63 0.4420742525316612 1102 64-65 0.3297689227416143 1102 66-67 0.4161246831026162 1102 68-69 0.35850594804451674 1102 70-71 0.4491594241992303 1102 72-73 0.4611446342822916 1102 74-75 0.40181204458849606 1102 76-77 0.38672865737704853 1102 78-79 0.41241753947472404 1102 80-81 0.26766198343021586 1102 82-83 0.512842977362979 1102 84-85 0.5226191403800797 1102 86-87 0.5596778807950926 1102 88-89 0.44882585265797914 1102 90-91 0.4677050371836238 1102 92-93 0.28585109661058183 1102 94-95 0.4641896887039145 1102 96-97 0.48293616226112945 1102 98-99 0.3938552037380454 1102 100-101 0.35268050771059833 1103 1 0.13406137756724235 1103 2 0.11770482909494717 1103 3 0.1568312927084392 1103 4 0.10256825540453463 1103 5 0.11542214209293888 1103 6 0.08341471704547843 1103 7 0.08450351004807999 1103 8 0.1137245657224284 1103 9 0.09753633741827628 1103 10-11 0.19878737371284672 1103 12-13 0.16248846876437995 1103 14-15 0.27165555041713674 1103 16-17 0.2887180593086356 1103 18-19 0.17893767495237256 1103 20-21 0.21396219311003506 1103 22-23 0.36039772844434736 1103 24-25 0.3204485630795162 1103 26-27 0.2744126750631324 1103 28-29 0.34054240546435466 1103 30-31 0.3634141437891927 1103 32-33 0.3423784019343401 1103 34-35 0.2711691255615918 1103 36-37 0.2658662627241739 1103 38-39 0.36769815830856345 1103 40-41 0.2854795677426267 1103 42-43 0.2128938824175819 1103 44-45 0.1778580079006744 1103 46-47 0.331843347153459 1103 48-49 0.31531684662671466 1103 50-51 0.41015233488358405 1103 52-53 0.3638217444931797 1103 54-55 0.39259608583026306 1103 56-57 0.3767884954996745 1103 58-59 0.5062349150180481 1103 60-61 0.22213355477788355 1103 62-63 0.28342444159356006 1103 64-65 0.22509307684997282 1103 66-67 0.27397701323706514 1103 68-69 0.35107786181519174 1103 70-71 0.2627126272559863 1103 72-73 0.2922017926727918 1103 74-75 0.38312393258077293 1103 76-77 0.1570438012656794 1103 78-79 0.12693376703317227 1103 80-81 0.15536624052996473 1103 82-83 0.36731846040239446 1103 84-85 0.31860760729883 1103 86-87 0.5335986397304779 1103 88-89 0.25601661886263116 1103 90-91 0.3553457645985141 1103 92-93 0.23160010548641097 1103 94-95 0.2923706016987886 1103 96-97 0.4080926957394375 1103 98-99 0.42957599077309894 1103 100-101 0.6719797875038829 1104 1 0.25339714340419306 1104 2 0.12694362652847246 1104 3 0.20042630603605005 1104 4 0.25425180291090044 1104 5 0.2963520891062501 1104 6 0.18294912002491515 1104 7 0.224361276933017 1104 8 0.24958345013614291 1104 9 0.24367001160587876 1104 10-11 0.26091214124407713 1104 12-13 0.2180642418916463 1104 14-15 0.4451518926687186 1104 16-17 0.33983540822341496 1104 18-19 0.30209785927268484 1104 20-21 0.3704560904676484 1104 22-23 0.5474186269518739 1104 24-25 0.34318523756808617 1104 26-27 0.2901210288305336 1104 28-29 0.3688688221898744 1104 30-31 0.28321976332819787 1104 32-33 0.4843654088637379 1104 34-35 0.31617094214538355 1104 36-37 0.3019095633669906 1104 38-39 0.32090553783547904 1104 40-41 0.3397789359551666 1104 42-43 0.31499218657965145 1104 44-45 0.288198686307787 1104 46-47 0.2816972410577776 1104 48-49 0.2926341300725426 1104 50-51 0.4999452323407283 1104 52-53 0.567970689975283 1104 54-55 0.6585490489002552 1104 56-57 0.673522062011223 1104 58-59 0.6082567787730682 1104 60-61 0.5867809756485727 1104 62-63 0.6512790621313869 1104 64-65 0.6358271685520833 1104 66-67 0.7446457798666017 1104 68-69 0.5914313822106863 1104 70-71 0.45971754222427563 1104 72-73 0.5351129191585784 1104 74-75 0.4719887535663361 1104 76-77 0.47738876033558597 1104 78-79 0.5407944212671296 1104 80-81 0.5442632564163787 1104 82-83 0.5507325707619515 1104 84-85 0.28507335060535155 1104 86-87 0.5672111354288347 1104 88-89 0.667009109117231 1104 90-91 0.6740644914790401 1104 92-93 0.5491998047608568 1104 94-95 0.48196829133994257 1104 96-97 0.7556174654289869 1104 98-99 0.8549329824623761 1104 100-101 1.2156245620376573 1105 1 0.25880672609353184 1105 2 0.27145676408643027 1105 3 0.2545221329294023 1105 4 0.2596613856002321 1105 5 0.24225626221289076 1105 6 0.24940970735103463 1105 7 0.3449176911524887 1105 8 0.30754326466473714 1105 9 0.23053245364606312 1105 10-11 0.3768317703012656 1105 12-13 0.3672141646119016 1105 14-15 0.32652747226686074 1105 16-17 0.4182743572187846 1105 18-19 0.46477173871626576 1105 20-21 0.4303478988138707 1105 22-23 0.4450229546180253 1105 24-25 0.5247926564243457 1105 26-27 0.4601364847810743 1105 28-29 0.4666277093614397 1105 30-31 0.5065582486450424 1105 32-33 0.5284148679054681 1105 34-35 0.29298701633394586 1105 36-37 0.31620631761737883 1105 38-39 0.40707246210133263 1105 40-41 0.4518345773771202 1105 42-43 0.5765841494853774 1105 44-45 0.5374258887807386 1105 46-47 0.4872613832525161 1105 48-49 0.5206094005516775 1105 50-51 0.38750319215525053 1105 52-53 0.4458686807017145 1105 54-55 0.6411611045416805 1105 56-57 0.6495653386727369 1105 58-59 0.6982876906741495 1105 60-61 0.7506140513827262 1105 62-63 0.6304135289010944 1105 64-65 0.4484237682429608 1105 66-67 0.4459595356625883 1105 68-69 0.4932860962756038 1105 70-71 0.5965027045117637 1105 72-73 0.5223617599622941 1105 74-75 0.5090830348646307 1105 76-77 0.5902171992845737 1105 78-79 0.49635856346187524 1105 80-81 0.47471147898206567 1105 82-83 0.5136382894636569 1105 84-85 0.7390918977460004 1105 86-87 0.6054646130177055 1105 88-89 0.6129132822238752 1105 90-91 0.729319514662965 1105 92-93 0.5155831123342729 1105 94-95 0.33359116614674633 1105 96-97 0.4182913448725749 1105 98-99 0.4635110350126119 1105 100-101 0.5293803580191074 1106 1 0.029336749218281 1106 2 0.02172245021938579 1106 3 0.06249792412968702 1106 4 0.07082777731035605 1106 5 0.11543699268999319 1106 6 0.05731330766441545 1106 7 0.03436433025249386 1106 8 0.023314724885189264 1106 9 0.01892202880861049 1106 10-11 0.04191457679892352 1106 12-13 0.008515399431097137 1106 14-15 0.07886420843402675 1106 16-17 0.1602309906145294 1106 18-19 0.17829107995667215 1106 20-21 0.1891616960308511 1106 22-23 0.165234746111814 1106 24-25 0.17583315196153393 1106 26-27 0.18981119974541372 1106 28-29 0.24990297439666875 1106 30-31 0.14984354939701205 1106 32-33 0.21411349817215353 1106 34-35 0.22070204458306364 1106 36-37 0.28897200966761716 1106 38-39 0.18793643316816855 1106 40-41 0.1435925083152867 1106 42-43 0.020161645810738094 1106 44-45 0.21442374198944947 1106 46-47 0.26566968070737573 1106 48-49 0.333008362896436 1106 50-51 0.24755879097273947 1106 52-53 0.14029471581252295 1106 54-55 0.06548020246329145 1106 56-57 0.2914967644309243 1106 58-59 0.21140749677652337 1106 60-61 0.30720460597442667 1106 62-63 0.24017267169940482 1106 64-65 0.10584783171413648 1106 66-67 0.12662196397466374 1106 68-69 0.2087037028044989 1106 70-71 0.31037175734365263 1106 72-73 0.12544206226458954 1106 74-75 0.18662291145372478 1106 76-77 0.29424876445401793 1106 78-79 0.21347383929023778 1106 80-81 0.3443335537718255 1106 82-83 0.22861150782807016 1106 84-85 0.24690981576081938 1106 86-87 0.08274223549960169 1106 88-89 0.0089282269209221 1106 90-91 0.11379516029802161 1106 92-93 0.2335232578048263 1106 94-95 0.09187595801213533 1106 96-97 0.08479252477936683 1106 98-99 0.1821032653796486 1106 100-101 0.5672474368444576 1107 1 0.08247193062148739 1107 2 0.08208170753648858 1107 3 0.17779331607188453 1107 4 0.0970033091533935 1107 5 -0.027830586099405252 1107 6 0.11958220170843958 1107 7 0.19725572902762423 1107 8 0.13863897628917243 1107 9 0.17787748822053828 1107 10-11 0.15943583148551 1107 12-13 0.1336300697393824 1107 14-15 0.2871039531935793 1107 16-17 0.08549020384419492 1107 18-19 0.23426891834613883 1107 20-21 0.27200063198863234 1107 22-23 0.2027053800743559 1107 24-25 0.19194931927763292 1107 26-27 0.24582925717206194 1107 28-29 0.24800285515183162 1107 30-31 0.187215938383261 1107 32-33 0.21275372629698097 1107 34-35 0.1987940288982557 1107 36-37 0.1428700633118325 1107 38-39 0.12152098709298542 1107 40-41 0.2598080258636841 1107 42-43 0.13000075738141703 1107 44-45 0.20539687076036728 1107 46-47 0.17055073674261934 1107 48-49 0.09250373985626936 1107 50-51 0.06202653039267858 1107 52-53 0.07519513838872882 1107 54-55 0.2133640670768031 1107 56-57 0.14505218872027115 1107 58-59 0.07958146652444498 1107 60-61 0.09753723890295163 1107 62-63 0.10857874520383604 1107 64-65 0.022434469660524314 1107 66-67 -0.10154923035266705 1107 68-69 0.026581770964405393 1107 70-71 0.06594869482668742 1107 72-73 -0.09781166398315122 1107 74-75 -0.2875457211358565 1107 76-77 -0.06855488927665476 1107 78-79 -0.09175088995274905 1107 80-81 -0.07033945624547755 1107 82-83 -0.07457248904773905 1107 84-85 -0.052600126016749016 1107 86-87 -0.06636477906889837 1107 88-89 -0.11521351110639344 1107 90-91 0.09660873677129445 1107 92-93 -0.23502514810902753 1107 94-95 -0.07599431195277973 1107 96-97 -0.018334919831961116 1107 98-99 -0.15210833710644067 1107 100-101 0.1041359693224031 1108 1 -0.03358749211447076 1108 2 -0.015573606035509613 1108 3 0.01888690274363114 1108 4 0.034901609535616274 1108 5 -0.02879983186435453 1108 6 0.03259954348043692 1108 7 -0.03153555518346707 1108 8 -0.028120578965136644 1108 9 0.02816035222313218 1108 10-11 -0.027799268718553094 1108 12-13 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70-71 -1.1651259455659897 2316 72-73 -1.0211888718854851 2316 74-75 -1.0132239491894666 2316 76-77 -1.445797177353029 2316 78-79 -1.5526057931753101 2316 80-81 -1.5078869669019888 2316 82-83 -1.4693211108549313 2316 84-85 -1.5467349009313693 2316 86-87 -1.5355579176397534 2316 88-89 -1.3961253718539623 2316 90-91 -1.4987622669607852 2316 92-93 -1.6386053792548978 2316 94-95 -1.4383690649587706 2316 96-97 -1.7406286084518747 2316 98-99 -1.5759365508531253 2316 100-101 -1.6593988112412994 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4971.0 3 933.0 4 804.0 5 734.0 6 740.0 7 771.0 8 789.0 9 965.0 10 1180.0 11 1594.0 12 2680.0 13 5060.0 14 5298.0 15 4784.0 16 5977.0 17 8699.0 18 7622.0 19 4469.0 20 3899.0 21 4064.0 22 4005.0 23 4434.0 24 4929.0 25 5746.0 26 6658.0 27 8170.0 28 10086.0 29 12434.0 30 16197.0 31 21610.0 32 29640.0 33 43059.0 34 67911.0 35 114645.0 36 205180.0 37 344447.0 38 319561.0 39 52673.0 40 57.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.117858769624995 17.65981778704225 11.948553747287786 24.273769696044972 2 16.0807086231469 19.27171115501206 39.424340900016006 25.223239321825037 3 20.472579304074223 23.643620680373026 27.66998857471003 28.21381144084272 4 12.499158831965282 14.491418590636043 35.64660773754959 37.36281483984909 5 13.907806191117093 37.472783011813966 33.28316135785853 15.336249439210409 6 34.32952105228528 34.150670837844466 16.769525478678542 14.750282631191716 7 30.797298398643964 31.438304388494643 19.31384768836327 18.450549524498122 8 28.117354477098576 33.88839704976282 18.9082567160965 19.085991757042105 9 27.539307417767915 15.425511630150233 19.102578769993936 37.93260218208792 10-11 26.234928937822605 25.813157895720646 26.247602256673215 21.70431090978354 12-13 26.431388998866083 22.826554688075394 27.408472493426604 23.33358381963192 14-15 23.15690853902684 24.286520216553455 25.386223261214898 27.170347983204806 16-17 23.120506811475643 27.80826619566859 25.876690530635564 23.194536462220203 18-19 22.85948219459763 26.210599121413214 28.1861482381531 22.74377044583606 20-21 23.473818245168566 25.457385346172796 28.251793165893922 22.817003242764716 22-23 22.96710285027877 25.357532607220527 28.30538909395164 23.369975448549063 24-25 22.892784340821635 25.56151040241205 28.417930046377514 23.1277752103888 26-27 22.740481727990836 25.903300734364702 28.28805131094361 23.068166226700846 28-29 22.58082245661683 26.01158230567072 28.412130874296203 22.995464363416247 30-31 22.63935436304442 25.681872903496927 28.46267906734682 23.21609366611184 32-33 22.461012054643128 25.75303665369717 28.504484739994997 23.281466551664707 34-35 23.038570990303032 25.970773865868146 28.06082715855614 22.929827985272684 36-37 22.972986661594376 25.72736948692928 28.059348833091164 23.240295018385186 38-39 22.717754061411117 25.940959852579702 28.300637460721433 23.040648625287744 40-41 22.794892085494116 25.893552970805434 28.318360268340736 22.993194675359714 42-43 22.835292051919676 25.823944940773213 28.04117886294773 23.299584144359386 44-45 22.81900247725927 26.054298581410723 27.989352513018556 23.13734642831145 46-47 22.864158022304537 26.381030899052437 27.620430352452814 23.134380726190212 48-49 23.231186419306464 26.31345685331764 27.378144978616923 23.077211748758973 50-51 22.913677475481908 26.42906013636529 27.455609220776733 23.201653167376065 52-53 23.18500063608147 26.521219790352053 27.188223448852483 23.105556124713996 54-55 22.898423144278002 26.42223020224864 27.35206588249774 23.327280770975616 56-57 22.910586591403185 26.842820939797967 27.17522548652484 23.071366982274007 58-59 23.150825353205544 26.290109194112777 27.206376197769593 23.352689254912086 60-61 22.963745601255265 26.434303136404893 27.59652100047688 23.005430261862962 62-63 22.866172351095628 26.49309591578075 27.593449580787276 23.047282152336344 64-65 22.784244416426514 26.531280019212293 27.37493245677233 23.309543107588855 66-67 22.834728382736877 26.726085229133627 27.161651279292382 23.277535108837114 68-69 22.80577396895824 26.52764091760897 27.392130212996534 23.274454900436258 70-71 22.721958715930214 26.53130672357502 27.367335775049916 23.379398785444845 72-73 22.905392247539623 26.637540002573974 27.208316558110806 23.248751191775593 74-75 22.667414672037793 27.000654817411014 27.018854613648347 23.313075896902845 76-77 22.860360064582142 26.823539388090577 27.007882908657844 23.30821763866944 78-79 22.94543382002282 26.815874084338247 27.059012460466565 23.179679635172366 80-81 22.726476224353757 27.01080914156122 27.009495849810456 23.25321878427457 82-83 22.993184414056227 27.049966454889706 26.907831244831275 23.049017886222792 84-85 22.905090190978314 26.95665812529291 26.719816049652785 23.41843563407599 86-87 22.68813472192777 26.829490684022222 27.110348980974052 23.37202561307595 88-89 22.842248893138624 26.90444328193874 26.89104611253172 23.362261712390918 90-91 22.747038384548137 27.013093378765852 26.994400884928183 23.245467351757824 92-93 22.792796613509108 26.958617270458017 27.00691957759689 23.241666538435986 94-95 22.768238563856034 26.784589286404426 27.060753500550266 23.386418649189274 96-97 22.863031515307483 26.775387831217596 27.013415454426433 23.34816519904849 98-99 22.69645025438387 26.758489684164882 26.863596754959858 23.681463306491395 100-101 22.688521736585958 26.85336737668258 26.793944075320535 23.664166811410926 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 137.0 1 115.0 2 217.0 3 360.0 4 290.5 5 267.5 6 574.0 7 1083.0 8 1672.5 9 1898.5 10 1597.0 11 1352.0 12 1398.0 13 1580.0 14 1702.0 15 1826.5 16 1975.0 17 2002.0 18 1999.0 19 2058.5 20 2205.5 21 2443.0 22 2794.5 23 3384.5 24 3879.5 25 4563.5 26 5880.0 27 7313.5 28 8359.0 29 9849.5 30 11997.0 31 14401.5 32 17028.0 33 19219.5 34 21611.5 35 24006.5 36 27103.0 37 30182.0 38 31813.0 39 32988.5 40 34404.5 41 36763.5 42 38331.5 43 40732.0 44 44067.0 45 46483.0 46 48372.5 47 49776.5 48 52292.5 49 52532.0 50 50515.5 51 49444.0 52 48892.5 53 49444.5 54 49652.5 55 47164.0 56 43011.0 57 39021.0 58 36187.0 59 33290.0 60 29377.5 61 24770.5 62 20440.0 63 16231.0 64 12229.5 65 9418.5 66 6947.0 67 5390.5 68 4047.0 69 2903.5 70 2382.5 71 1786.5 72 1419.5 73 1335.0 74 1064.5 75 775.5 76 544.5 77 307.5 78 189.0 79 103.0 80 68.5 81 50.5 82 31.0 83 26.5 84 23.0 85 16.5 86 14.5 87 16.5 88 18.0 89 16.0 90 12.5 91 11.0 92 9.5 93 10.0 94 9.5 95 10.5 96 12.0 97 8.5 98 3.5 99 3.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.034991308248752316 2 0.009046898072861175 3 0.006803865492812949 4 0.0036636198807454344 5 0.005607581450120564 6 0.0032150133647357894 7 0.003439316622740612 8 0.006803865492812949 9 0.009869343352212191 10-11 0.0014579711770313464 12-13 0.005719733079122974 14-15 0.005121591057776781 16-17 0.012934821211611432 18-19 0.007476775266827417 20-21 0.00269163909605787 22-23 0.005944036337127797 24-25 0.01413110525430382 26-27 0.004523449036430587 28-29 0.01319650834595039 30-31 0.009645040094207368 32-33 0.007962765659171199 34-35 0.20011589001663585 36-37 0.3547356025346268 38-39 0.35043645675620105 40-41 0.36752088824090173 42-43 0.3589599805603843 44-45 0.3703994467186303 46-47 0.3779883736144601 48-49 0.38228751939288586 50-51 0.39211947886876386 52-53 0.38120338697919587 54-55 0.3724555599170078 56-57 0.36725920110656274 58-59 0.3659133815585338 60-61 0.3626609843174639 62-63 0.36322174246247596 64-65 0.3734649245780295 66-67 0.35338978298659784 68-69 0.3747359763733902 70-71 0.35933381932372566 72-73 0.3666984429615507 74-75 0.3770163928297725 76-77 0.3669975139722238 78-79 0.3663993719508776 80-81 0.3697639208209499 82-83 0.36942746593394266 84-85 0.36946484981027683 86-87 0.3702872950896278 88-89 0.3816146096188714 90-91 0.40296080300566367 92-93 0.3918204078580908 94-95 0.3689414755415989 96-97 0.3474083627731359 98-99 0.34008112301164506 100-101 0.3487541823211649 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1337475.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.54723563023184 #Duplication Level Percentage of deduplicated Percentage of total 1 76.88709209364968 41.939783293556445 2 12.894622558799828 14.067320301555144 3 4.165948126725274 6.817228622754194 4 1.8676425079941261 4.07498943826371 5 1.0488742598166376 2.860659569835157 6 0.6317394663375415 2.067578491633847 7 0.4311727491021367 1.6463497079826361 8 0.32065939055898873 1.3992866667094157 9 0.23495811166952965 1.153468393242494 >10 1.3558915568527812 13.74469812463637 >50 0.10607530591597117 3.945868932305007 >100 0.05285284933045771 4.758172212224176 >500 0.001921906969942275 0.6749109017142645 >1k 5.491162771263643E-4 0.8496853435872361 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4800 0.35888521280771607 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3234 0.24179891212919866 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2258 0.1688255855249631 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.476775266827417E-5 7 0.0 0.0 0.0 0.0 7.476775266827417E-5 8 0.0 0.0 0.0 0.0 7.476775266827417E-5 9 0.0 0.0 0.0 0.0 7.476775266827417E-5 10-11 0.0 0.0 0.0 0.0 7.476775266827417E-5 12-13 0.0 0.0 0.0 7.476775266827417E-5 7.476775266827417E-5 14-15 0.0 0.0 0.0 7.476775266827417E-5 7.476775266827417E-5 16-17 0.0 0.0 0.0 7.476775266827417E-5 7.476775266827417E-5 18-19 0.0 0.0 0.0 7.476775266827417E-5 1.4953550533654833E-4 20-21 0.0 0.0 0.0 1.1215162900241125E-4 2.616871343389596E-4 22-23 0.0 0.0 0.0 2.616871343389596E-4 2.9907101067309667E-4 24-25 0.0 0.0 0.0 4.1122263967550797E-4 2.9907101067309667E-4 26-27 0.0 0.0 0.0 4.859903923437821E-4 2.9907101067309667E-4 28-29 7.476775266827417E-5 0.0 0.0 5.233742686779192E-4 3.738387633413709E-4 30-31 7.476775266827417E-5 0.0 0.0 9.719807846875644E-4 3.738387633413709E-4 32-33 7.476775266827417E-5 0.0 0.0 0.0019065776930409914 4.4860651600964505E-4 34-35 7.476775266827417E-5 0.0 0.0 0.0036636198807454344 6.729097740144676E-4 36-37 7.476775266827417E-5 0.0 0.0 0.006579562234808127 6.729097740144676E-4 38-39 7.476775266827417E-5 0.0 0.0 0.012598366324604197 6.729097740144676E-4 40-41 7.476775266827417E-5 0.0 0.0 0.019850838333426792 6.729097740144676E-4 42-43 7.476775266827417E-5 0.0 0.0 0.027477149105590758 6.729097740144676E-4 44-45 7.476775266827417E-5 0.0 0.0 0.03603805678610815 7.476775266827418E-4 46-47 7.476775266827417E-5 0.0 0.0 0.047103684181012725 8.224452793510159E-4 48-49 7.476775266827417E-5 0.0 0.0 0.05917867623693901 8.224452793510159E-4 50-51 7.476775266827417E-5 0.0 0.0 0.07502943980261313 9.719807846875643E-4 52-53 7.476775266827417E-5 0.0 0.0 0.09144096151329931 9.719807846875643E-4 54-55 7.476775266827417E-5 0.0 0.0 0.10792725097665376 9.719807846875643E-4 56-57 7.476775266827417E-5 0.0 0.0 0.13002112189012877 9.719807846875643E-4 58-59 7.476775266827417E-5 0.0 0.0 0.15828333239873643 9.719807846875643E-4 60-61 7.476775266827417E-5 0.0 0.0 0.19118114357277707 0.0010467485373558383 62-63 7.476775266827417E-5 0.0 0.0 0.22594814856352455 0.0011962840426923867 64-65 7.476775266827417E-5 0.0 0.0 0.2576122918185387 0.0011962840426923867 66-67 7.476775266827417E-5 0.0 0.0 0.2968279780930485 0.0011962840426923867 68-69 7.476775266827417E-5 0.0 0.0 0.3487167984448307 0.0011962840426923867 70-71 7.476775266827417E-5 0.0 0.0 0.4068487261444139 0.0011962840426923867 72-73 7.476775266827417E-5 0.0 0.0 0.46337314716162914 0.0012336679190265239 74-75 7.476775266827417E-5 0.0 0.0 0.5252434624946261 0.001271051795360661 76-77 7.476775266827417E-5 0.0 0.0 0.5910390848427074 0.001271051795360661 78-79 7.476775266827417E-5 0.0 0.0 0.6722742481167872 0.001271051795360661 80-81 7.476775266827417E-5 0.0 0.0 0.7677900521505074 0.001271051795360661 82-83 1.4953550533654833E-4 0.0 0.0 0.8688012860053459 0.0013458195480289352 84-85 1.8691938167068542E-4 0.0 0.0 0.9678685582908091 0.0013458195480289352 86-87 2.2430325800482253E-4 0.0 0.0 1.073889231574422 0.0013832034243630724 88-89 2.2430325800482253E-4 0.0 0.0 1.1992747527991177 0.0014205873006972094 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3030 0.0 38.001423 1 GTATCAA 4890 0.0 35.853294 1 TACCTGG 1355 0.0 34.265575 2 GTACCTG 1440 0.0 32.905895 1 GTACATG 6695 0.0 31.919949 1 TACATGG 6690 0.0 31.30166 2 ACATGGG 6705 0.0 29.95864 3 ATCAACG 5860 0.0 29.427866 3 TCAACGC 5905 0.0 29.283834 4 CAACGCA 6005 0.0 28.717285 5 TATCAAC 6190 0.0 28.013132 2 AACGCAG 6210 0.0 27.76929 6 CATGGGG 3030 0.0 25.329544 4 CATGGGA 3660 0.0 25.111673 4 GTCTACG 115 1.3032707E-4 24.722342 1 AGTACAT 4545 0.0 23.87112 2 GAGTACA 5190 0.0 23.829193 1 CGCAGAG 7440 0.0 23.115587 8 ACGCAGA 7480 0.0 22.927778 7 ATGGGAG 1755 0.0 22.40557 5 >>END_MODULE