FastQCFastQC Report
Thu 26 May 2016
SRR936355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936355_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337475
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52540.3928297725191125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37680.2817248920540571No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28060.20979831398717733No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13650.10205798239219424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61000.033.749071
GGTATCA41450.030.0524941
TACCTGG12700.028.7671492
GTACCTG13450.028.6329781
GTACATG69500.028.1848811
TACATGG70000.027.8582362
ATCAACG76250.026.8172823
TCAACGC76300.026.799714
TATCAAC78200.026.3325442
ACATGGG69700.026.2062763
CAACGCA78250.026.0712265
AACGCAG80550.025.4445956
GAGTACA54750.023.707311
AGTACAT49900.023.580932
CATGGGA38000.023.3472294
TAGTCGA1252.2744025E-422.7729155
CATGGGG31200.022.6573434
ACGCAGA95400.021.483887
CGCAGAG95800.021.3446548
AGTACTT53850.020.66019612-13
Produced by FastQC (version 0.11.5)