FastQCFastQC Report
Thu 26 May 2016
SRR936354_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936354_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3175139
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA52010.16380385236677827No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA47390.1492533082803619No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44100.13889155718851992No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39080.12308122573531427No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA38480.12119154468513031No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG37350.11763264537395055No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA32510.10238921823580006No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA31960.1006570106064648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32700.050.9603841
GTATCAA61750.046.2293851
ATCAACG77450.035.9916423
TCAACGC78150.035.6686974
CAACGCA79800.034.9311875
TATCAAC83200.033.503712
AACGCAG84050.033.050196
TACATGG120200.029.4114952
GTACATG126850.028.5128541
ACATGGG120050.028.266033
ACGCAGA99850.027.7730247
CGCAGAG103750.026.6390578
GAGTACA86450.024.3687231
CATGGGG76750.023.740144
GTAAGGT12850.021.7295064
TAAGGTG15350.021.5819475
GCAGAGT127750.021.4862799
AGTACAT89150.021.4467242
GTACCTG29150.020.3006171
TACCTGG27350.020.0725082