##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936354_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3175139 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.62704183974308 33.0 31.0 34.0 30.0 34.0 2 31.714542575931322 33.0 31.0 34.0 30.0 34.0 3 31.7819619865461 34.0 31.0 34.0 30.0 34.0 4 35.277196368410955 37.0 35.0 37.0 33.0 37.0 5 35.24787166798052 37.0 35.0 37.0 33.0 37.0 6 35.334385675713726 37.0 35.0 37.0 33.0 37.0 7 35.27229264608573 37.0 35.0 37.0 33.0 37.0 8 35.2659341842987 37.0 35.0 37.0 33.0 37.0 9 36.92223301090125 39.0 37.0 39.0 33.0 39.0 10-11 36.83671203055992 39.0 37.0 39.0 33.0 39.0 12-13 36.72832575203795 39.0 37.0 39.0 33.0 39.0 14-15 37.966020857669534 40.0 38.0 41.0 33.0 41.0 16-17 37.90178178026222 40.0 38.0 41.0 33.0 41.0 18-19 37.819445857330976 40.0 38.0 41.0 32.5 41.0 20-21 37.71764385748151 40.0 38.0 41.0 32.0 41.0 22-23 37.55149160398962 40.0 38.0 41.0 32.0 41.0 24-25 37.70441451539602 40.0 38.0 41.0 32.0 41.0 26-27 37.65562972203736 40.0 38.0 41.0 32.0 41.0 28-29 37.518655561221095 40.0 38.0 41.0 32.0 41.0 30-31 37.35619306745311 40.0 37.0 41.0 31.5 41.0 32-33 37.180036527534696 40.0 37.0 41.0 31.0 41.0 34-35 36.90928664855302 40.0 37.0 41.0 30.5 41.0 36-37 36.704865991693595 40.0 36.0 41.0 30.0 41.0 38-39 36.52540014783605 40.0 36.0 41.0 30.0 41.0 40-41 36.31261025107877 40.0 35.5 41.0 30.0 41.0 42-43 36.103096116421995 39.0 35.0 41.0 29.0 41.0 44-45 35.92113258663636 39.0 35.0 41.0 28.5 41.0 46-47 35.63830355143507 39.0 35.0 41.0 27.5 41.0 48-49 35.46176624078505 39.0 35.0 41.0 27.0 41.0 50-51 35.13246050015448 38.5 34.5 40.5 26.5 41.0 52-53 35.12763393980547 38.5 35.0 40.0 27.0 41.0 54-55 35.1616340261009 39.0 35.0 41.0 27.0 41.0 56-57 34.95735068606446 38.0 35.0 40.5 26.0 41.0 58-59 34.68117836730927 38.0 34.0 40.0 26.0 41.0 60-61 34.350029872707935 37.0 34.0 40.0 26.0 41.0 62-63 34.010195459159426 37.0 34.0 40.0 25.5 41.0 64-65 33.65631299921043 36.0 34.0 39.5 24.5 41.0 66-67 33.28498012213009 35.5 33.0 39.0 23.5 41.0 68-69 32.897107654184595 35.0 33.0 39.0 22.5 40.5 70-71 32.48604281576334 35.0 33.0 37.5 22.0 40.0 72-73 32.101511461387986 35.0 32.5 37.0 20.5 39.5 74-75 31.709860103762388 35.0 32.0 36.5 20.0 39.0 76-77 31.337540025806746 35.0 32.0 36.0 18.5 39.0 78-79 30.975737912576427 35.0 31.5 35.5 17.5 37.0 80-81 30.61371943086586 35.0 31.0 35.0 13.5 37.0 82-83 30.29297630749394 34.5 31.0 35.0 8.5 36.5 84-85 30.01646274383578 34.0 31.0 35.0 7.0 36.0 86-87 29.714717371428463 34.0 30.5 35.0 2.0 36.0 88-89 29.40200098326404 34.0 30.0 35.0 2.0 35.5 90-91 29.06351674682589 34.0 30.0 35.0 2.0 35.0 92-93 28.75784587698365 34.0 29.0 35.0 2.0 35.0 94-95 28.367139674829986 34.0 29.0 35.0 2.0 35.0 96-97 27.922825583383908 34.0 29.0 35.0 2.0 35.0 98-99 27.40251292935522 34.0 28.0 35.0 2.0 35.0 100-101 24.075305679530878 29.5 14.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4204276747773257 1101 2 0.34973041758636825 1101 3 0.389400730280844 1101 4 0.2801482156407147 1101 5 0.5189539675281765 1101 6 0.3351835079638761 1101 7 0.5288585422004886 1101 8 0.4348050484981769 1101 9 0.46702747418675017 1101 10-11 0.628908284868416 1101 12-13 0.48583620418854423 1101 14-15 0.6615408368268092 1101 16-17 0.6506854572861016 1101 18-19 0.710845811015993 1101 20-21 0.7909173273949364 1101 22-23 0.7503655435657564 1101 24-25 0.6896943194210152 1101 26-27 0.6045482278906817 1101 28-29 0.6122620574840383 1101 30-31 0.6512102857719597 1101 32-33 0.7393695426147815 1101 34-35 0.6377157179591322 1101 36-37 0.6520386877717925 1101 38-39 0.6883143223911503 1101 40-41 0.6771969619383142 1101 42-43 0.6372482075254027 1101 44-45 0.6628829960740177 1101 46-47 0.5395785960477042 1101 48-49 0.7153080086497994 1101 50-51 0.7692128877637288 1101 52-53 0.7896796190961552 1101 54-55 0.7842362914569847 1101 56-57 0.8164825573150054 1101 58-59 0.8043980713110699 1101 60-61 0.7002771140938364 1101 62-63 0.7543836104569763 1101 64-65 0.7162290977464281 1101 66-67 0.7588156070939789 1101 68-69 0.6496115733186159 1101 70-71 0.8161187985072118 1101 72-73 0.8024491914252039 1101 74-75 0.6761220323316444 1101 76-77 0.595733652499522 1101 78-79 0.6722694815302752 1101 80-81 0.7591750405265678 1101 82-83 0.8878706484436663 1101 84-85 0.8332894462957263 1101 86-87 0.8389027480543625 1101 88-89 0.6341664479529072 1101 90-91 0.6912414068806498 1101 92-93 0.8093674303262084 1101 94-95 0.9048659195282021 1101 96-97 0.9057139749432217 1101 98-99 0.9898128700649416 1101 100-101 1.062248685430209 1102 1 0.44393323868558454 1102 2 0.4782676351796802 1102 3 0.37071210800811727 1102 4 0.4347593706105144 1102 5 0.48538283347645717 1102 6 0.4161456643365966 1102 7 0.3847231478996562 1102 8 0.3712922688571041 1102 9 0.46153559754309725 1102 10-11 0.4903029200842113 1102 12-13 0.5573332424696389 1102 14-15 0.6240861551487384 1102 16-17 0.6528387375532105 1102 18-19 0.6848991546407603 1102 20-21 0.6090027948587675 1102 22-23 0.6983827255581048 1102 24-25 0.7370282695695991 1102 26-27 0.6824693425831185 1102 28-29 0.6637962806739424 1102 30-31 0.6562675842217089 1102 32-33 0.5239264915917587 1102 34-35 0.5358221537893897 1102 36-37 0.6313818926634056 1102 38-39 0.5940737091292903 1102 40-41 0.5832816223463198 1102 42-43 0.5559588918383511 1102 44-45 0.5023519860222692 1102 46-47 0.5660624725304473 1102 48-49 0.5698902504045051 1102 50-51 0.37699544530103424 1102 52-53 0.6288095701699703 1102 54-55 0.7383227972823718 1102 56-57 0.7736040707583882 1102 58-59 0.6238021699478722 1102 60-61 0.6319882484472572 1102 62-63 0.7986534512080183 1102 64-65 0.6134005681971288 1102 66-67 0.681191292840019 1102 68-69 0.6464566387127562 1102 70-71 0.7527413452837735 1102 72-73 0.6605495429093367 1102 74-75 0.765619684075741 1102 76-77 0.7776762811381879 1102 78-79 0.6718274236133333 1102 80-81 0.7118601982418689 1102 82-83 0.7573608581776128 1102 84-85 0.8174233823648365 1102 86-87 0.7321041197978566 1102 88-89 0.6618577025853085 1102 90-91 0.7851174622129982 1102 92-93 0.799489607516449 1102 94-95 0.7411416962880075 1102 96-97 0.9518609747571709 1102 98-99 0.9442000104725459 1102 100-101 0.9131765482156311 1103 1 0.2990778317340741 1103 2 0.2949066851658735 1103 3 0.30663154620593147 1103 4 0.25063107140351093 1103 5 0.1771471448348052 1103 6 0.2535406223197896 1103 7 0.2523830287534068 1103 8 0.33605930243109583 1103 9 0.3618433262683922 1103 10-11 0.34189975600168054 1103 12-13 0.42157506595960115 1103 14-15 0.37978653389178163 1103 16-17 0.44533587725415913 1103 18-19 0.40622514982756286 1103 20-21 0.47998801993472284 1103 22-23 0.4400632005650067 1103 24-25 0.43539444702650343 1103 26-27 0.4747475214603014 1103 28-29 0.45572382634009756 1103 30-31 0.4470736953985721 1103 32-33 0.41640390509236624 1103 34-35 0.302117474734672 1103 36-37 0.3882791586120362 1103 38-39 0.45063760229218985 1103 40-41 0.5152834371567465 1103 42-43 0.34942508113096693 1103 44-45 0.44897486052418856 1103 46-47 0.5312136738560511 1103 48-49 0.5283444067149148 1103 50-51 0.5605171638476065 1103 52-53 0.6007081183216272 1103 54-55 0.6008869074334768 1103 56-57 0.5810078069007361 1103 58-59 0.5423309899265689 1103 60-61 0.5693047947841023 1103 62-63 0.6504588916940719 1103 64-65 0.5558262004218406 1103 66-67 0.6177547914789088 1103 68-69 0.6272447990473182 1103 70-71 0.5687353485188638 1103 72-73 0.6098117830166458 1103 74-75 0.6035625372733584 1103 76-77 0.5052263856870454 1103 78-79 0.5751276169300965 1103 80-81 0.4960126620442935 1103 82-83 0.5095790694057634 1103 84-85 0.5820838944569964 1103 86-87 0.6495223546974493 1103 88-89 0.6347129714534212 1103 90-91 0.7635537663121035 1103 92-93 0.7864609453473541 1103 94-95 0.7449217294280253 1103 96-97 0.8048795765640904 1103 98-99 0.8090173458574021 1103 100-101 1.1016702555577247 1104 1 0.24561374889115584 1104 2 0.14671904290262816 1104 3 0.21471212256323824 1104 4 0.21886554052701968 1104 5 0.2337416221021158 1104 6 0.11238231607617877 1104 7 0.18039372665985098 1104 8 0.127807141766624 1104 9 0.22024574683013753 1104 10-11 0.18785966080410788 1104 12-13 0.26020102616110563 1104 14-15 0.36875024717592453 1104 16-17 0.35955216659456823 1104 18-19 0.1480352135016716 1104 20-21 0.2951922880008837 1104 22-23 0.3855185442594973 1104 24-25 0.41809375306652186 1104 26-27 0.2650999869384023 1104 28-29 0.2387928238323056 1104 30-31 0.19819967972272678 1104 32-33 0.2509045327633501 1104 34-35 0.21999551985557986 1104 36-37 0.2449499960824042 1104 38-39 0.20053315127995575 1104 40-41 0.29449034037963173 1104 42-43 0.2830576798818001 1104 44-45 0.2425121772765806 1104 46-47 0.2905333938211996 1104 48-49 0.27690108911918543 1104 50-51 0.28109671499289846 1104 52-53 0.18005530673195125 1104 54-55 0.23397245305372394 1104 56-57 0.2050766731658129 1104 58-59 0.08888255061285122 1104 60-61 0.17433649916409877 1104 62-63 0.2445316224053684 1104 64-65 0.19403394648961836 1104 66-67 0.22039636481509461 1104 68-69 0.21969449852752376 1104 70-71 0.19321604848055784 1104 72-73 0.2408728364424988 1104 74-75 0.225378590597888 1104 76-77 0.2680985858933447 1104 78-79 0.253811517290103 1104 80-81 0.2547572983762656 1104 82-83 0.289205465262075 1104 84-85 0.09691521451516039 1104 86-87 0.1811488185795902 1104 88-89 0.19200760821959761 1104 90-91 0.22244710663135336 1104 92-93 0.18263374447045067 1104 94-95 -0.009856542542149072 1104 96-97 0.2713027372854917 1104 98-99 0.37913485204003194 1104 100-101 0.651018775537537 1105 1 0.27032347038277393 1105 2 0.2617909725131007 1105 3 0.2595998497743359 1105 4 0.18233709289454936 1105 5 0.2251433871399371 1105 6 0.22755477092507448 1105 7 0.2469420286210351 1105 8 0.3488083137293003 1105 9 0.2680950473776704 1105 10-11 0.3557624155877548 1105 12-13 0.2978408361517708 1105 14-15 0.3446155752013027 1105 16-17 0.41844623212394794 1105 18-19 0.3295032313257735 1105 20-21 0.45412167995270636 1105 22-23 0.542275592938303 1105 24-25 0.4271368062675407 1105 26-27 0.35164496895257713 1105 28-29 0.4001376732862596 1105 30-31 0.5412169839661587 1105 32-33 0.43801354929499325 1105 34-35 0.19385782903437843 1105 36-37 0.36914096694351173 1105 38-39 0.3780389181134467 1105 40-41 0.39319525210694195 1105 42-43 0.4249164366935432 1105 44-45 0.46304405322368325 1105 46-47 0.5071270212577943 1105 48-49 0.40198448882571114 1105 50-51 0.3674478353918289 1105 52-53 0.5128922917495302 1105 54-55 0.5662376562186751 1105 56-57 0.6239675399488362 1105 58-59 0.637099433695937 1105 60-61 0.538358706448804 1105 62-63 0.3736173168440544 1105 64-65 0.45569508208972564 1105 66-67 0.34757831799196737 1105 68-69 0.4503378945124794 1105 70-71 0.4718956554149898 1105 72-73 0.43405947105357257 1105 74-75 0.4374940919580119 1105 76-77 0.5747960383152595 1105 78-79 0.4211525308625568 1105 80-81 0.5849269186748529 1105 82-83 0.5156778956213159 1105 84-85 0.519354111270868 1105 86-87 0.5072128265398597 1105 88-89 0.6328930644633743 1105 90-91 0.6839496238617464 1105 92-93 0.6165626997434934 1105 94-95 0.6530175738368484 1105 96-97 0.7239062161954521 1105 98-99 0.726499516373071 1105 100-101 0.7004726775268573 1106 1 0.1022447740054524 1106 2 0.08608529204274973 1106 3 0.14243910087379064 1106 4 0.16017274593453834 1106 5 0.13334794563785834 1106 6 0.08532937201343316 1106 7 0.07631375759875425 1106 8 0.11082827807364737 1106 9 0.17153557393513807 1106 10-11 0.1136049768094054 1106 12-13 0.13353116921886965 1106 14-15 0.14693606048082586 1106 16-17 0.23662963243641855 1106 18-19 0.10428205445330718 1106 20-21 0.21616910819301438 1106 22-23 0.15411603591302736 1106 24-25 0.14846042266248105 1106 26-27 0.14948548570399822 1106 28-29 0.25931767420441787 1106 30-31 0.2568174135361829 1106 32-33 0.2621077582058575 1106 34-35 0.10365737497233596 1106 36-37 0.1877617659358961 1106 38-39 0.25206370407718737 1106 40-41 0.25697415467741536 1106 42-43 0.3360343074564298 1106 44-45 0.3372362244059701 1106 46-47 0.39190834562351284 1106 48-49 0.48681207973785945 1106 50-51 0.5386770164577683 1106 52-53 0.4283327484619761 1106 54-55 0.3401620341787748 1106 56-57 0.3828197318047941 1106 58-59 0.47620575505219165 1106 60-61 0.49464907765313626 1106 62-63 0.4755445075696585 1106 64-65 0.39765022470135136 1106 66-67 0.4909261518769057 1106 68-69 0.5272023432450155 1106 70-71 0.6037612680385323 1106 72-73 0.5322033511088549 1106 74-75 0.4607266399241361 1106 76-77 0.48991612145396246 1106 78-79 0.5557116289459003 1106 80-81 0.46453450759109316 1106 82-83 0.5968248041059496 1106 84-85 0.5586962289650543 1106 86-87 0.5867166424024752 1106 88-89 0.5051022267197212 1106 90-91 0.5050766912715581 1106 92-93 0.4906455357104882 1106 94-95 0.7606961669267953 1106 96-97 0.885466429139516 1106 98-99 0.9477894822681172 1106 100-101 0.9814454475654664 1107 1 0.11395930449302938 1107 2 0.1853515042824796 1107 3 0.2035632488232828 1107 4 0.12208655380878497 1107 5 0.16895713847132043 1107 6 0.271718912744241 1107 7 0.2338360133442734 1107 8 0.27974845537465143 1107 9 0.32419695137023297 1107 10-11 0.23521074281678267 1107 12-13 0.2633035832688506 1107 14-15 0.41139763905571414 1107 16-17 0.12609586702501474 1107 18-19 0.2920404632140645 1107 20-21 0.468776475869106 1107 22-23 0.4765729212260936 1107 24-25 0.41146932496068445 1107 26-27 0.33780261610962725 1107 28-29 0.45370624648437996 1107 30-31 0.31208429624188483 1107 32-33 0.44946574978778386 1107 34-35 0.4209833331799615 1107 36-37 0.4725049402197996 1107 38-39 0.503423260079721 1107 40-41 0.5655188710330563 1107 42-43 0.09185432459055676 1107 44-45 0.33869821271007083 1107 46-47 0.4041815977559722 1107 48-49 0.3658954037591684 1107 50-51 0.3662927649585299 1107 52-53 0.3183577046954511 1107 54-55 0.46661005444174464 1107 56-57 0.47547440458014734 1107 58-59 0.526481391063669 1107 60-61 0.54032457212179 1107 62-63 0.6038642453429617 1107 64-65 0.34688105123690605 1107 66-67 0.4620309308093766 1107 68-69 0.37387981186348185 1107 70-71 0.518118551906376 1107 72-73 0.46532437021713235 1107 74-75 0.48210705486807726 1107 76-77 0.44179156377526496 1107 78-79 0.4507650799832561 1107 80-81 0.525276862416586 1107 82-83 0.6152719304142877 1107 84-85 0.5160657674609759 1107 86-87 0.2791217528431176 1107 88-89 0.44362422172847715 1107 90-91 0.7386782109944328 1107 92-93 0.428999914225777 1107 94-95 0.7635642713704947 1107 96-97 0.9038669953107821 1107 98-99 0.8602284884885485 1107 100-101 0.9131603505341452 1108 1 -0.11519989890215498 1108 2 -0.020391583978916827 1108 3 -0.03949227791655474 1108 4 -0.02896614207471515 1108 5 -0.027684331729766143 1108 6 0.06296976857112924 1108 7 0.05879526791617451 1108 8 0.016979748785530546 1108 9 0.013452145691921658 1108 10-11 0.013774613436503103 1108 12-13 0.0015956409648367753 1108 14-15 -0.03419021315882986 1108 16-17 0.1600358288960564 1108 18-19 -0.014233180084204378 1108 20-21 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-1.6873175055329455 2316 62-63 -1.665913139121777 2316 64-65 -1.4477056930805858 2316 66-67 -1.4702980737648517 2316 68-69 -1.4605011538518369 2316 70-71 -1.3779287500427841 2316 72-73 -1.4478018111247586 2316 74-75 -1.297868145747401 2316 76-77 -1.6169397095454094 2316 78-79 -1.658855877046399 2316 80-81 -1.4221902408133005 2316 82-83 -1.5508163006727962 2316 84-85 -1.7961056144197975 2316 86-87 -1.8366417812312754 2316 88-89 -1.8367378792115403 2316 90-91 -2.018900947262228 2316 92-93 -2.0415824794225017 2316 94-95 -2.068068713414096 2316 96-97 -2.2877401355543334 2316 98-99 -2.0478644705490012 2316 100-101 -1.910555739798891 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29541.0 3 6786.0 4 5865.0 5 4809.0 6 4731.0 7 4982.0 8 5923.0 9 7172.0 10 8345.0 11 9934.0 12 12081.0 13 16349.0 14 16687.0 15 14428.0 16 16477.0 17 19171.0 18 17996.0 19 15357.0 20 15233.0 21 16132.0 22 17205.0 23 18885.0 24 21932.0 25 25768.0 26 29753.0 27 35701.0 28 43406.0 29 52430.0 30 63901.0 31 79200.0 32 100092.0 33 132545.0 34 185851.0 35 282714.0 36 473585.0 37 711251.0 38 566240.0 39 86630.0 40 51.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.88956816091458 16.380524813787492 11.629163687718183 25.100743337579743 2 17.048106492157384 18.256981846553245 37.557724672251744 27.137186989037627 3 20.728091212672712 21.556736094181026 27.074336765966724 30.640835927179538 4 13.407068482916515 13.686819422060134 33.396504593591054 39.5096075014323 5 15.107373001766689 36.51312007715599 32.506684531981975 15.872822389095354 6 37.38358967122901 32.30018116672378 15.509534560261242 14.806694601785967 7 32.7361231627866 30.259229571163328 18.14925109882409 18.855396167225987 8 29.62794975494828 32.942825465220295 17.888460520845147 19.54076425898628 9 27.605367080282246 15.070966051141419 18.031100388794233 39.2925664797821 10-11 27.5304796429685 24.670101761535967 25.22731782291414 22.57210077258139 12-13 27.551514723914593 21.367543038783648 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24.662994844440114 38-39 24.463333271106517 24.866468110392056 25.92081711612884 24.749381502372582 40-41 24.608556624947308 24.779118842575773 26.208857146112273 24.403467386364643 42-43 24.606177401529518 24.835391386003344 25.821516242517163 24.73691496994998 44-45 24.47323158947313 24.97875962542249 26.009817808303502 24.538190976800873 46-47 24.90727387160725 25.2744277187228 25.529124874562363 24.289173535107583 48-49 24.930687641271707 25.309321930623383 25.380731306712452 24.379259121392458 50-51 24.661621384113484 25.417780640214733 25.501005168056405 24.419592807615377 52-53 25.105099553962923 25.034038755514924 25.524484876473704 24.336376814048453 54-55 24.66108347895797 25.245702510503886 25.543946200617185 24.54926780992096 56-57 24.59080984822328 25.582616709269047 25.2906872786873 24.535886163820372 58-59 24.629775410242026 25.120206590614018 25.50515883156678 24.744859167577175 60-61 24.259872103519726 25.350939998781634 25.776132899680455 24.613054998018182 62-63 24.358824855717693 25.31943054473246 26.010514115734384 24.31123048381546 64-65 24.272939039173053 25.401178008399878 25.740955256569727 24.58492769585735 66-67 24.24086276953044 25.519885975755134 25.642671823851593 24.596579430862832 68-69 24.561407395638224 25.103511969154983 26.008296149626943 24.326784485579854 70-71 24.370183210655465 25.158906878267196 25.731197898298014 24.739712012779318 72-73 24.367024262019495 25.528659291964683 25.533612048521825 24.570704397494 74-75 24.31337248305419 25.733875270046507 25.47205419240526 24.48069805449404 76-77 24.53574463304371 25.541869933493572 25.4524509904758 24.46993444298692 78-79 24.507567234505945 25.700969627576804 25.465738654686437 24.32572448323081 80-81 24.690832319020284 25.755892668273113 25.330941390112077 24.222333622594526 82-83 24.576006603057174 25.897345943830658 25.377225434239463 24.149422018872706 84-85 24.510655778302464 25.91970066123599 25.15135226058777 24.418291299873772 86-87 24.584949189061568 25.65626806439275 25.41361871454078 24.345164032004902 88-89 24.34379670252017 26.01121169097565 25.079325538653208 24.565666067850973 90-91 24.290870407659284 26.09082734951813 25.19026316122741 24.428039081595177 92-93 24.41546753337817 25.698519443473206 25.420338353677618 24.465674669471007 94-95 24.423411414422215 25.48700990444941 25.71667692050616 24.372901760622216 96-97 24.654755870763783 25.14038198326806 25.521615408417603 24.68324673755055 98-99 24.142078701735205 25.475276630423778 25.31647672052481 25.066167947316202 100-101 24.300322561763696 25.416364116704397 25.30663933038772 24.976673991144185 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 85.0 1 71.5 2 145.0 3 262.0 4 224.5 5 214.5 6 446.5 7 789.5 8 1124.0 9 1273.5 10 1215.5 11 1223.5 12 1380.5 13 1618.5 14 1939.5 15 2230.0 16 2518.0 17 2760.0 18 2930.0 19 3128.5 20 3271.0 21 3489.5 22 3810.5 23 4243.5 24 5090.5 25 6380.5 26 8244.0 27 10833.5 28 13288.0 29 16153.0 30 19711.5 31 24735.0 32 33509.0 33 36604.0 34 35186.5 35 38027.0 36 43165.5 37 48487.0 38 53646.5 39 58487.5 40 63203.0 41 69376.0 42 73486.0 43 78353.5 44 85193.0 45 93974.5 46 100882.0 47 105548.5 48 111222.5 49 115419.0 50 118787.0 51 121653.0 52 127129.0 53 136381.0 54 145908.5 55 144034.0 56 134276.5 57 126947.0 58 122197.0 59 113529.5 60 97995.0 61 84337.0 62 73367.5 63 57678.0 64 43223.5 65 34091.5 66 24985.0 67 20093.5 68 15532.0 69 10968.5 70 8713.5 71 5960.0 72 4402.0 73 3762.0 74 3016.5 75 2343.5 76 1699.5 77 1095.5 78 725.0 79 441.0 80 299.0 81 219.5 82 132.5 83 75.5 84 57.5 85 51.0 86 43.0 87 38.5 88 36.5 89 37.5 90 32.0 91 32.0 92 33.0 93 25.0 94 27.0 95 32.5 96 33.5 97 29.0 98 24.0 99 20.5 100 18.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046612132571203974 2 0.011653033142800993 3 0.009983814881805175 4 0.005417085677193975 5 0.008566554094167216 6 0.005133633519666383 7 0.0048501813621387915 8 0.009416910566749991 9 0.01423559724471905 10-11 0.0021731332077115366 12-13 0.00850356472582775 14-15 0.008361838647063956 16-17 0.01834565352886913 18-19 0.011353833643188534 20-21 0.003306941837821903 22-23 0.009747604750532182 24-25 0.02037706065781687 26-27 0.007117798622359525 28-29 0.01751104439837122 30-31 0.014534796744331509 32-33 0.011983727326583182 34-35 0.2617838148188158 36-37 0.46500326442401424 38-39 0.4616490805599377 40-41 0.4817111943760572 42-43 0.47215255773054343 44-45 0.48730150081618473 46-47 0.4944822888068837 48-49 0.500450531457048 50-51 0.5106705564701262 52-53 0.4998993744840777 54-55 0.48736449018452427 56-57 0.48186866779690596 58-59 0.48081359587721983 60-61 0.4775066540393979 62-63 0.475616972989214 64-65 0.4903407378385639 66-67 0.46481429631899573 68-69 0.4913485677319953 70-71 0.4734753344656722 72-73 0.4813175108239356 74-75 0.49539563464780595 76-77 0.48167969969188745 78-79 0.480797848535135 80-81 0.4839158222679385 82-83 0.4847189367142667 84-85 0.48421502176755093 86-87 0.4861361975019046 88-89 0.4991120073798344 90-91 0.5234101562167829 92-93 0.5110169979959932 94-95 0.4833489179528834 96-97 0.4559485427252161 98-99 0.4485787866294988 100-101 0.45679889919779887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3175139.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.83177531041253 #Duplication Level Percentage of deduplicated Percentage of total 1 68.93463082609574 29.525926186495706 2 15.047725512978548 12.890415962093188 3 5.981179095823095 7.685535573708936 4 3.0819135678489555 5.280153178568732 5 1.7937700103799268 3.8415177021574696 6 1.1410569214433803 2.9324096205392345 7 0.7719320363382264 2.314425367474368 8 0.5576924460949457 1.9109566034762455 9 0.3996303598307193 1.5405190001539812 >10 2.0521660613439874 15.639280721666129 >50 0.14230679415528244 4.20040810385445 >100 0.0855313315947677 6.939354611667923 >500 0.006264282156379077 1.7414918440188751 >1k 0.00412705647891702 3.393431170676906 >5k 7.36974371235182E-5 0.16417435344772321 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5201 0.16380385236677827 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4739 0.1492533082803619 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4410 0.13889155718851992 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3908 0.12308122573531427 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3848 0.12119154468513031 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3735 0.11763264537395055 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3251 0.10238921823580006 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3196 0.1006570106064648 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.149468416973241E-5 2 0.0 0.0 0.0 0.0 3.149468416973241E-5 3 0.0 0.0 0.0 3.149468416973241E-5 3.149468416973241E-5 4 0.0 0.0 0.0 9.448405250919724E-5 3.149468416973241E-5 5 0.0 0.0 0.0 9.448405250919724E-5 3.149468416973241E-5 6 0.0 0.0 0.0 9.448405250919724E-5 9.448405250919724E-5 7 0.0 0.0 0.0 9.448405250919724E-5 9.448405250919724E-5 8 0.0 0.0 0.0 1.2597873667892964E-4 9.448405250919724E-5 9 0.0 0.0 0.0 1.2597873667892964E-4 9.448405250919724E-5 10-11 0.0 0.0 0.0 1.4172607876379584E-4 9.448405250919724E-5 12-13 0.0 0.0 0.0 1.5747342084866206E-4 4.5667292046111996E-4 14-15 0.0 0.0 0.0 2.0471544710326067E-4 6.613883675643806E-4 16-17 0.0 0.0 0.0 2.677048154427255E-4 8.503564725827751E-4 18-19 0.0 0.0 0.0 3.306941837821903E-4 9.133458409222399E-4 20-21 0.0 0.0 0.0 4.251782362913875E-4 0.001055071919686036 22-23 0.0 0.0 0.0 6.141463413097821E-4 0.001070819261770902 24-25 0.0 0.0 0.0 7.716197621584441E-4 0.0011810506563649653 26-27 0.0 0.0 0.0 0.0010393245776011695 0.0012912820509590288 28-29 0.0 0.0 0.0 0.001448755471807691 0.0012912820509590288 30-31 0.0 0.0 0.0 0.0028502689173607832 0.001307029393043895 32-33 0.0 0.0 0.0 0.005637548466382102 0.0014015134455530923 34-35 0.0 0.0 0.0 0.009889330829295977 0.001448755471807691 36-37 0.0 0.0 0.0 0.01692839274123117 0.001448755471807691 38-39 0.0 0.0 0.0 0.03072306440757397 0.0014959974980622895 40-41 0.0 0.0 0.0 0.045068893046887076 0.0015117448401471558 42-43 0.0 0.0 0.0 0.06012335208001918 0.0015589868664017544 44-45 0.0 0.0 0.0 0.07848475295097317 0.0016534709189109517 46-47 0.0 0.0 0.0 0.09953894931843929 0.0017322076293352826 48-49 0.0 0.0 0.0 0.12429377107584896 0.0018266916818444798 50-51 0.0 0.0 0.0 0.15375704811663365 0.0018896810501839447 52-53 0.0 0.0 0.0 0.1821495058956474 0.0021573858656266705 54-55 0.0 0.0 0.0 0.21172301433102614 0.0022361225760510013 56-57 0.0 0.0 0.0 0.24868202620420712 0.0023306066285601985 58-59 0.0 0.0 0.0 0.28957787359860465 0.002393595996899663 60-61 0.0 0.0 0.0 0.3370246153003066 0.0024250906810693957 62-63 0.0 0.0 0.0 0.3821879923997028 0.0025510694177483255 64-65 0.0 0.0 0.0 0.43188660401954054 0.0026298061281726567 66-67 0.0 0.0 0.0 0.48988406491810277 0.0026455534702575227 68-69 0.0 0.0 0.0 0.559770769090739 0.0026455534702575227 70-71 0.0 0.0 0.0 0.637326428858705 0.0027872795490213186 72-73 0.0 0.0 0.0 0.7185197246482753 0.003102226390718643 74-75 0.0 0.0 0.0 0.7996657784115908 0.003354183864076502 76-77 0.0 0.0 0.0 0.8894098809532434 0.003511657284925164 78-79 0.0 0.0 0.0 0.9973894056291709 0.0035431519690948964 80-81 0.0 0.0 0.0 1.1181872667621797 0.0036376360216040936 82-83 1.5747342084866205E-5 0.0 0.0 1.246669830832603 0.0039683302053862834 84-85 3.149468416973241E-5 0.0 0.0 1.3730107563794844 0.0039683302053862834 86-87 3.149468416973241E-5 0.0 0.0 1.5087370978089463 0.004141550968319812 88-89 3.149468416973241E-5 0.0 0.0 1.6603997494282927 0.004330519073338207 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3270 0.0 50.960384 1 GTATCAA 6175 0.0 46.229385 1 ATCAACG 7745 0.0 35.991642 3 TCAACGC 7815 0.0 35.668697 4 CAACGCA 7980 0.0 34.931187 5 TATCAAC 8320 0.0 33.50371 2 AACGCAG 8405 0.0 33.05019 6 TACATGG 12020 0.0 29.411495 2 GTACATG 12685 0.0 28.512854 1 ACATGGG 12005 0.0 28.26603 3 ACGCAGA 9985 0.0 27.773024 7 CGCAGAG 10375 0.0 26.639057 8 GAGTACA 8645 0.0 24.368723 1 CATGGGG 7675 0.0 23.74014 4 GTAAGGT 1285 0.0 21.729506 4 TAAGGTG 1535 0.0 21.581947 5 GCAGAGT 12775 0.0 21.486279 9 AGTACAT 8915 0.0 21.446724 2 GTACCTG 2915 0.0 20.300617 1 TACCTGG 2735 0.0 20.072508 2 >>END_MODULE