FastQCFastQC Report
Thu 26 May 2016
SRR936354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936354_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3175139
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA53650.16896898057061438No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48810.15372555343246389No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46070.14509600996995722No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39900.12566378983723234No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG37400.11779011879479921No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36630.11536502811372984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35670.11234153843343551No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA33120.10431039397015374No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA32560.10254669165664873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38200.044.8326341
GTATCAA66850.042.19961
ATCAACG85850.032.324953
TCAACGC86050.032.3049474
CAACGCA88450.031.3747545
TATCAAC89800.031.119292
AACGCAG92700.030.0386686
TACATGG123650.028.355742
GTACATG130700.027.4442161
ACATGGG122400.027.4393673
ACGCAGA113550.024.3140957
CGCAGAG115000.024.0075268
TAAGGTG15650.022.4305365
GAGTACA92900.021.7634771
CATGGGA49300.021.746224
GTAAGGT14200.021.7143734
CATGGGG78700.021.5789624
AGTACAT93050.020.2935182
AGGTAAG13600.020.2339082
GCAGAGT142300.019.2683819