##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936354_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3175139 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.497416333584134 34.0 31.0 34.0 31.0 34.0 2 32.63482606588247 34.0 31.0 34.0 31.0 34.0 3 32.71760889838209 34.0 31.0 34.0 31.0 34.0 4 36.20243365723516 37.0 35.0 37.0 35.0 37.0 5 36.15833480046071 37.0 35.0 37.0 35.0 37.0 6 36.19221867137155 37.0 37.0 37.0 35.0 37.0 7 36.137365324793656 37.0 36.0 37.0 35.0 37.0 8 36.10868595044185 37.0 36.0 37.0 35.0 37.0 9 37.879430790274064 39.0 38.0 39.0 35.0 39.0 10-11 37.82010882043274 39.0 38.0 39.0 35.0 39.0 12-13 37.759250697371044 39.0 38.0 39.0 35.0 39.0 14-15 39.13616411753942 41.0 39.0 41.0 36.0 41.0 16-17 39.062559780847394 40.0 38.0 41.0 36.0 41.0 18-19 39.060415780222534 40.0 38.5 41.0 35.5 41.0 20-21 39.00071083502171 40.0 38.5 41.0 35.0 41.0 22-23 38.8963078466801 40.0 38.0 41.0 35.0 41.0 24-25 39.029935382356484 40.0 39.0 41.0 35.0 41.0 26-27 38.951548735346705 40.0 38.0 41.0 35.0 41.0 28-29 38.86140968946556 40.0 38.0 41.0 35.0 41.0 30-31 38.74645110025104 40.0 38.0 41.0 35.0 41.0 32-33 38.605467193719704 40.0 38.0 41.0 34.0 41.0 34-35 38.46803053346641 40.0 38.0 41.0 34.0 41.0 36-37 38.32162560442236 40.0 38.0 41.0 34.0 41.0 38-39 38.17437504310835 40.0 38.0 41.0 33.0 41.0 40-41 38.01430834996515 40.0 37.5 41.0 33.0 41.0 42-43 38.030903843894706 40.0 38.0 41.0 33.0 41.0 44-45 37.90779726493864 40.0 37.0 41.0 33.0 41.0 46-47 37.709154622836984 40.0 37.0 41.0 33.0 41.0 48-49 37.47238388618577 40.0 37.0 41.0 32.5 41.0 50-51 37.248575416698294 40.0 36.0 41.0 32.0 41.0 52-53 37.005520073294434 39.5 36.0 41.0 31.5 41.0 54-55 36.772194225197694 39.0 35.0 41.0 31.0 41.0 56-57 36.47941696410771 39.0 35.0 41.0 31.0 41.0 58-59 36.17748167875485 38.5 35.0 40.5 31.0 41.0 60-61 35.861539762511185 38.0 35.0 40.0 30.0 41.0 62-63 35.53523357560094 37.0 35.0 40.0 30.0 41.0 64-65 35.14264210165287 36.5 34.0 39.5 29.0 41.0 66-67 34.779200217691255 36.0 34.0 39.0 29.0 41.0 68-69 34.42123651909412 35.5 34.0 39.0 29.0 40.5 70-71 34.04620333157068 35.0 34.0 38.0 28.0 40.0 72-73 33.66338292591285 35.0 34.0 37.0 28.0 39.5 74-75 33.26417473376756 35.0 33.0 37.0 27.0 39.0 76-77 32.58656739122287 35.0 32.5 36.0 26.0 39.0 78-79 32.66148978044741 35.0 33.0 36.0 26.5 37.5 80-81 32.5330730402669 35.0 33.0 35.5 27.0 37.0 82-83 32.291395116875194 35.0 33.0 35.0 26.0 37.0 84-85 32.04508731743712 35.0 33.0 35.0 26.0 36.0 86-87 31.676430543670687 35.0 32.5 35.0 25.0 36.0 88-89 31.209637278871888 35.0 32.0 35.0 23.5 36.0 90-91 31.016497073041528 34.0 32.0 35.0 23.0 35.0 92-93 30.75267775678482 34.0 31.5 35.0 20.5 35.0 94-95 30.6053953858398 34.0 31.0 35.0 20.0 35.0 96-97 30.36017021617006 34.0 31.0 35.0 18.5 35.0 98-99 29.92191507206456 34.0 31.0 35.0 4.5 35.0 100-101 26.627532841869282 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15814391002102468 1101 2 0.17227265570831207 1101 3 0.21083029239390072 1101 4 0.15688771834248172 1101 5 0.13192401910391993 1101 6 0.19870382237085948 1101 7 0.181160372518832 1101 8 0.14678562068525736 1101 9 0.18389538908366632 1101 10-11 0.22737421871941166 1101 12-13 0.32664263887133416 1101 14-15 0.3901646606178133 1101 16-17 0.3746867675396004 1101 18-19 0.35137519804904827 1101 20-21 0.2759834127064025 1101 22-23 0.27496164017589564 1101 24-25 0.20910870005884874 1101 26-27 0.29432168947634096 1101 28-29 0.36403123505220236 1101 30-31 0.33035710330049284 1101 32-33 0.4265402644626164 1101 34-35 0.3399942614070426 1101 36-37 0.3271476747935438 1101 38-39 0.31392092640429325 1101 40-41 0.3744860919617352 1101 42-43 0.378875071908098 1101 44-45 0.4125865430133686 1101 46-47 0.4333166194181217 1101 48-49 0.362547368460298 1101 50-51 0.2980359004201105 1101 52-53 0.4230124359276388 1101 54-55 0.42686476105640025 1101 56-57 0.2878473394336254 1101 58-59 0.16621061304827123 1101 60-61 0.31319197394933695 1101 62-63 0.39587110825551974 1101 64-65 0.403561641799449 1101 66-67 0.39704435343144695 1101 68-69 0.5361607637785681 1101 70-71 0.45275349855481295 1101 72-73 0.43891345693451456 1101 74-75 0.4248572788406264 1101 76-77 0.2670083325403567 1101 78-79 0.27959911038561813 1101 80-81 0.44856374187268955 1101 82-83 0.4787384429160966 1101 84-85 0.5632066766282549 1101 86-87 0.5171718986530145 1101 88-89 0.5007738163557534 1101 90-91 0.619660740430362 1101 92-93 0.6379198951142371 1101 94-95 0.5653891803918221 1101 96-97 0.5253644188265802 1101 98-99 0.5479220977775441 1101 100-101 0.8535625102713631 1102 1 0.08098874410945456 1102 2 0.1510343907023497 1102 3 0.13659510978809664 1102 4 0.15502168458190368 1102 5 0.12144323004970659 1102 6 0.14530589104854386 1102 7 0.1505543515303529 1102 8 0.11559479849398713 1102 9 0.14021606932362118 1102 10-11 0.13806488000235362 1102 12-13 0.24963876450051714 1102 14-15 0.26051189576018885 1102 16-17 0.30893082524534776 1102 18-19 0.3551231678385065 1102 20-21 0.2822745511831073 1102 22-23 0.3270211410583741 1102 24-25 0.24560685715566422 1102 26-27 0.2878910761641933 1102 28-29 0.25699979255932703 1102 30-31 0.2686382629467019 1102 32-33 0.2778950371544582 1102 34-35 0.2456088306504256 1102 36-37 0.2249042607210967 1102 38-39 0.17371345522951742 1102 40-41 0.24916358390374427 1102 42-43 0.25284990986838096 1102 44-45 0.32742914198456674 1102 46-47 0.38981228746423824 1102 48-49 0.3290790020318113 1102 50-51 0.2686975982041062 1102 52-53 0.13842047681847447 1102 54-55 0.14824212376891666 1102 56-57 0.31994723082051024 1102 58-59 0.1542427419157235 1102 60-61 0.18235502436895956 1102 62-63 0.25591166218243444 1102 64-65 0.2131803770599987 1102 66-67 0.22988832012342897 1102 68-69 0.2025266492017721 1102 70-71 0.2687095705814002 1102 72-73 0.14545658036629305 1102 74-75 0.1914060089219376 1102 76-77 0.048736311984320935 1102 78-79 0.08177105853290811 1102 80-81 0.03957663465242689 1102 82-83 0.1139607729692429 1102 84-85 0.13848242070355354 1102 86-87 0.038329520930776795 1102 88-89 -0.04025164738707332 1102 90-91 0.15691434955386185 1102 92-93 0.07685707046911361 1102 94-95 0.022836910327310278 1102 96-97 -0.10925519640138148 1102 98-99 -0.04087202001740664 1102 100-101 0.4159611254540714 1103 1 -0.044530054959473375 1103 2 0.039718455877718384 1103 3 0.06519415503999682 1103 4 -0.011237636047759736 1103 5 0.022823077763440835 1103 6 0.025359743575471327 1103 7 0.10615264718664008 1103 8 0.13665843521628318 1103 9 0.16208157608970453 1103 10-11 0.13696070593836396 1103 12-13 0.12744432334377365 1103 14-15 0.1606051020567918 1103 16-17 0.174150081962118 1103 18-19 0.0823009403559567 1103 20-21 0.17149960740277237 1103 22-23 0.1603643374918775 1103 24-25 0.10438550946165037 1103 26-27 0.15717935485595547 1103 28-29 0.18071216483217967 1103 30-31 0.10187740367342002 1103 32-33 0.2007907447332684 1103 34-35 0.23596222552950508 1103 36-37 0.17914687325907863 1103 38-39 0.2486296188780699 1103 40-41 0.3070313789090662 1103 42-43 0.327385357850396 1103 44-45 0.27759780099746223 1103 46-47 0.3832656547266424 1103 48-49 0.31299573774017375 1103 50-51 0.2997224729427259 1103 52-53 0.26057201348947956 1103 54-55 0.2516882909681968 1103 56-57 0.2217068078907687 1103 58-59 0.19592420323264292 1103 60-61 0.26956603553399106 1103 62-63 0.2273579871913185 1103 64-65 0.21004144543100978 1103 66-67 0.09284202273123299 1103 68-69 0.03650059089113 1103 70-71 -0.054906143309793265 1103 72-73 -0.01954075659666188 1103 74-75 0.11193901085510305 1103 76-77 0.09011497612602426 1103 78-79 0.11956497891796403 1103 80-81 0.12242815554247244 1103 82-83 0.2006978621711184 1103 84-85 0.1529239719719513 1103 86-87 0.11441495510444355 1103 88-89 0.2203728841156689 1103 90-91 0.28742732939368665 1103 92-93 0.3486816453694992 1103 94-95 0.30139107847343993 1103 96-97 0.20245822136009295 1103 98-99 -0.06673208235219974 1103 100-101 0.023619822735799545 1104 1 -0.0035218156320482308 1104 2 0.033960382755253704 1104 3 0.05433040547541168 1104 4 0.04031906999669843 1104 5 0.0706869701340338 1104 6 -0.007266241713580257 1104 7 0.005895596381478185 1104 8 0.04803404298608882 1104 9 -0.026912399040824653 1104 10-11 0.03347110134695441 1104 12-13 0.1256570772707022 1104 14-15 0.21300652249406937 1104 16-17 0.11453430489000738 1104 18-19 0.14369624425774674 1104 20-21 0.0960229561842425 1104 22-23 0.14044205579931912 1104 24-25 0.2376802586393154 1104 26-27 0.24104947713842506 1104 28-29 0.258253231207064 1104 30-31 0.17165996706047082 1104 32-33 0.08209067255834412 1104 34-35 0.024992348429506706 1104 36-37 0.12236948610718201 1104 38-39 0.12142951389635925 1104 40-41 0.21088500613330297 1104 42-43 0.17355724031333608 1104 44-45 0.14640835484352976 1104 46-47 0.20133436515948944 1104 48-49 0.1002138285590064 1104 50-51 0.15346645072674647 1104 52-53 0.09679288661310181 1104 54-55 -0.001175246799654417 1104 56-57 0.011834872551020226 1104 58-59 0.07692320117836715 1104 60-61 -0.0024924864624793486 1104 62-63 0.023617362453649093 1104 64-65 -0.11370736170466245 1104 66-67 -0.22449004271637563 1104 68-69 -0.10164654705464216 1104 70-71 -0.07890557256823172 1104 72-73 -0.0023461419268215877 1104 74-75 -0.005918698883981222 1104 76-77 -0.04482680630516711 1104 78-79 -0.056002699066077355 1104 80-81 0.09852015107303913 1104 82-83 0.05706770945553785 1104 84-85 0.14596260147818185 1104 86-87 0.14131350301827084 1104 88-89 0.2846202675333842 1104 90-91 0.399151678106648 1104 92-93 0.36008459614678046 1104 94-95 0.35331818758784195 1104 96-97 0.16074505831326746 1104 98-99 0.0920949689643642 1104 100-101 0.1591538158714627 1105 1 -0.01661861442602941 1105 2 0.061818706809411594 1105 3 0.11785273140603891 1105 4 0.03480004428826078 1105 5 -0.03763943079982823 1105 6 0.05405878787066598 1105 7 0.020943606688760497 1105 8 0.0610351883321556 1105 9 0.0846672885395705 1105 10-11 0.11315202758602538 1105 12-13 0.12225104216790328 1105 14-15 0.19921501825507448 1105 16-17 0.11119897016175173 1105 18-19 0.16351320277041026 1105 20-21 0.10515483044405016 1105 22-23 -0.016768485281929202 1105 24-25 0.143742809815393 1105 26-27 0.16357131315704265 1105 28-29 0.125138150127988 1105 30-31 0.12278254047804893 1105 32-33 0.13189896760157893 1105 34-35 0.08402312115370592 1105 36-37 0.09611409995658704 1105 38-39 0.09600720392381845 1105 40-41 0.13552428866352528 1105 42-43 0.2118340666198648 1105 44-45 0.29227089168941944 1105 46-47 0.2481765121361832 1105 48-49 0.3226846771310363 1105 50-51 0.33120439996672957 1105 52-53 0.3562331392712892 1105 54-55 0.2936822099569625 1105 56-57 0.3195827899496919 1105 58-59 0.29775112751736543 1105 60-61 0.3450814866264622 1105 62-63 0.30300961728108433 1105 64-65 0.2682549170908004 1105 66-67 0.3292900686622673 1105 68-69 0.3174465584468322 1105 70-71 0.2900306672198667 1105 72-73 0.25337717221680833 1105 74-75 0.3225394376418862 1105 76-77 0.314204370421173 1105 78-79 0.24240676827254504 1105 80-81 0.3240133515387811 1105 82-83 0.3340915611477797 1105 84-85 0.2666830751302882 1105 86-87 0.35533969685350897 1105 88-89 0.43922998542463176 1105 90-91 0.536581026747065 1105 92-93 0.6383443973278169 1105 94-95 0.4452668764642489 1105 96-97 0.4178221955113237 1105 98-99 0.4274678397691112 1105 100-101 0.48663034602065736 1106 1 -0.0037389145006088143 1106 2 0.021816357056295033 1106 3 0.011412384202259318 1106 4 -0.01734331331698513 1106 5 -0.01655247802374049 1106 6 0.03275212392687621 1106 7 -0.011698650221354967 1106 8 0.04005335000419308 1106 9 -0.010616819393192145 1106 10-11 0.12100039958988162 1106 12-13 0.032121011316981196 1106 14-15 0.12052627300589336 1106 16-17 0.07342894369118369 1106 18-19 0.1408510365593756 1106 20-21 0.1496716086873704 1106 22-23 0.11129930417047973 1106 24-25 0.06201592166676306 1106 26-27 -0.024896232670378993 1106 28-29 0.06080515912007911 1106 30-31 0.09067210023122385 1106 32-33 0.13761030158718768 1106 34-35 0.21788400027359955 1106 36-37 0.10738031044890306 1106 38-39 0.15846790887057693 1106 40-41 0.1069340838730568 1106 42-43 -0.0161143618497519 1106 44-45 -0.013367000947447139 1106 46-47 0.10716484396988335 1106 48-49 -0.05579012725952737 1106 50-51 0.06722900622986572 1106 52-53 0.09701217956596508 1106 54-55 0.1524166597545289 1106 56-57 0.08148815757446215 1106 58-59 0.2324367214145795 1106 60-61 0.15243913951913157 1106 62-63 0.10938238595517902 1106 64-65 0.13128998172115303 1106 66-67 0.05017611292111468 1106 68-69 0.12764669566223574 1106 70-71 0.056051456290560964 1106 72-73 -0.014695372247821581 1106 74-75 0.0812409538917862 1106 76-77 0.04614616500117563 1106 78-79 -0.09464866497069835 1106 80-81 -0.05873622271301571 1106 82-83 -0.022162233929002184 1106 84-85 0.09270311024476285 1106 86-87 0.07351641332098424 1106 88-89 -0.032936367845252335 1106 90-91 0.1682922633659345 1106 92-93 0.28834363990247525 1106 94-95 0.2557033766411152 1106 96-97 0.27126050761149756 1106 98-99 0.3346631723638325 1106 100-101 0.6442060391197693 1107 1 0.02907667010847348 1107 2 0.062323676309837595 1107 3 0.07154945724580841 1107 4 0.04394845112596357 1107 5 0.05105284100631735 1107 6 0.07024234629808745 1107 7 0.07490320473450396 1107 8 0.09449146226999261 1107 9 0.09438560173393284 1107 10-11 0.0542704111367911 1107 12-13 0.1280628297696964 1107 14-15 0.19451033081496405 1107 16-17 0.19291156744803573 1107 18-19 0.16573277381121443 1107 20-21 0.11888604605466924 1107 22-23 0.07718934775076747 1107 24-25 0.1161746731101303 1107 26-27 0.15122214182212446 1107 28-29 0.15918672875541517 1107 30-31 0.02577658117881043 1107 32-33 -0.016028838726441563 1107 34-35 0.13557498995587025 1107 36-37 0.18758480257093169 1107 38-39 0.2245926155417095 1107 40-41 0.17454871493053048 1107 42-43 0.08281661740304713 1107 44-45 0.11609811619785404 1107 46-47 0.1493390161306536 1107 48-49 0.0011436083533880037 1107 50-51 0.05913685440656735 1107 52-53 0.06207345270635045 1107 54-55 0.05627928872610255 1107 56-57 -0.058369182165549205 1107 58-59 -0.001909760501469293 1107 60-61 0.12918700706484998 1107 62-63 -0.005108991107434235 1107 64-65 0.10814028156843847 1107 66-67 0.015730923514475137 1107 68-69 0.12727421456565224 1107 70-71 0.19653270186734062 1107 72-73 0.08371413346976198 1107 74-75 0.10894423545024523 1107 76-77 -0.028769095549350254 1107 78-79 -0.02032006214729165 1107 80-81 0.1082749889679917 1107 82-83 0.35418335403241485 1107 84-85 0.19024659741278072 1107 86-87 0.2409746023178947 1107 88-89 0.3476461925898029 1107 90-91 0.04961978599416028 1107 92-93 0.21854068240256908 1107 94-95 0.29958164981875157 1107 96-97 0.21086269780034783 1107 98-99 0.20275830403589978 1107 100-101 0.5434685265485335 1108 1 -0.12204034246209261 1108 2 -0.08078994292154107 1108 3 -0.0745176580239928 1108 4 -0.023122096672395287 1108 5 -0.05346730809774414 1108 6 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-0.7199914881100113 2316 74-75 -0.7160966144194205 2316 76-77 -0.6311959242723901 2316 78-79 -0.7013764104744382 2316 80-81 -0.7992978278284681 2316 82-83 -0.8462544828242642 2316 84-85 -0.6946149743893741 2316 86-87 -0.7763980149363299 2316 88-89 -0.810932351293367 2316 90-91 -0.9178140548519309 2316 92-93 -0.8743997404695101 2316 94-95 -0.8233365120341958 2316 96-97 -0.8787266374311464 2316 98-99 -0.9600802849607035 2316 100-101 -1.045809257731019 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 11.0 4 31.0 5 58.0 6 118.0 7 275.0 8 603.0 9 953.0 10 1552.0 11 2580.0 12 3793.0 13 5354.0 14 7065.0 15 9093.0 16 11180.0 17 14536.0 18 14030.0 19 10410.0 20 9987.0 21 10299.0 22 11341.0 23 12921.0 24 15551.0 25 18971.0 26 23582.0 27 29377.0 28 37421.0 29 46603.0 30 57683.0 31 72198.0 32 91091.0 33 118456.0 34 167628.0 35 257313.0 36 458693.0 37 801958.0 38 734992.0 39 117369.0 40 60.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.02809788533223 16.79269914774127 12.086833884705701 25.0923690822208 2 17.274022814128983 18.59409473672273 37.224824887132684 26.907057562015595 3 20.77981467897941 21.5673077619594 27.420027910589113 30.232849648472083 4 13.583279346195553 14.001717720074616 33.256276339398056 39.158726594331775 5 15.032664711686639 36.50555141050518 32.62808336894857 15.833700508859613 6 37.36598618202226 32.53136319386332 15.340714217550792 14.761936406563619 7 32.54736249342155 30.380906158753994 18.190857156174893 18.880874191649564 8 29.491212825643228 33.15886328126108 17.815125574029988 19.534798319065715 9 27.380407597903588 15.051876469030177 18.07136632443493 39.496349608631306 10-11 27.43484825484091 24.722955876396714 25.24748624149089 22.594709627271484 12-13 27.418584996267086 21.391741740866888 26.407100923062675 24.782572339803345 14-15 23.54435506603018 23.308948049203515 23.700710425590817 29.445986459175487 16-17 24.756903080117382 26.52233102817293 24.526801721823333 24.19396416988636 18-19 24.774206735516145 24.932609249547816 26.07221290154541 24.220971113390625 20-21 24.80689809605066 24.31923409919005 26.41982062506279 24.4540471796965 22-23 24.409809024018646 24.227391727129927 26.571022514772984 24.791776734078447 24-25 24.31766432755703 24.591028508518793 26.55424599500767 24.537061168916512 26-27 24.18839482391862 25.0156689656215 26.236273513684438 24.559662696775437 28-29 24.107056807079672 25.343001176040026 26.25627468062041 24.29366733625989 30-31 24.14645534507208 24.824607748265997 26.237682464869188 24.79125444179273 32-33 23.849965851367074 24.84201631418065 26.442805331060644 24.865212503391636 34-35 24.504277909558798 24.921809315356523 26.288353040645614 24.285559734439065 36-37 24.788040575269846 24.6366139907078 25.981894948751798 24.59345048527055 38-39 24.340567512788034 24.817192841061427 26.162458263592214 24.679781382558318 40-41 24.43854754145758 24.759213294141027 26.367061581745276 24.435177582656113 42-43 24.511051402082565 24.841835990686064 25.983779035579857 24.663333571651517 44-45 24.31866567622094 24.982633866472188 26.218948243016577 24.479752214290293 46-47 24.79114680422009 25.251297910554577 25.7086141194273 24.248941165798033 48-49 24.778602034262626 25.318036622523042 25.53750528000484 24.365856063209492 50-51 24.600985339593176 25.379959218204025 25.625821667518125 24.39323377468467 52-53 24.999342503014642 25.062288980870225 25.638571308658374 24.299797207456756 54-55 24.5581435193438 25.165646969600573 25.729041934083874 24.54716757697175 56-57 24.520709391988866 25.56187180261646 25.462849958284213 24.454568847110462 58-59 24.57775199372364 25.102619170675215 25.641249741345835 24.67837909425531 60-61 24.199172811370506 25.338320905346993 25.875628170774263 24.586878112508238 62-63 24.303219481099884 25.28209001243725 26.097140944065757 24.31754956239711 64-65 24.199180153226404 25.37959762281915 25.86606964713456 24.55515257681989 66-67 24.13755744236709 25.515686084924155 25.774005484484302 24.572750988224453 68-69 24.488518455412503 25.08113818009227 26.137847823355138 24.292495541140088 70-71 24.292691269092895 25.166827604756207 25.832405813423936 24.708075312726958 72-73 24.24782612940644 25.492496764665773 25.666396963620326 24.593280142307464 74-75 24.267598993303917 25.658545972318063 25.571888348825045 24.50196668555298 76-77 24.41344768887274 25.55320588746217 25.52291247206734 24.510433951597747 78-79 24.446180220299908 25.676675402470984 25.5586829297129 24.318461447516206 80-81 24.588501408773638 25.74703111900027 25.388585941491016 24.275881530735074 82-83 24.48349505328743 25.863261419421324 25.458428749103586 24.194814778187666 84-85 24.449685200328997 25.920167603404536 25.20027230514049 24.429874891125973 86-87 24.534107238188277 25.66141336754878 25.433240300358502 24.371239093904443 88-89 24.228798288809088 26.049979851943462 25.09511720086472 24.626104658382733 90-91 24.250626623423805 26.066049615559344 25.23752297513915 24.445800785877704 92-93 24.323424107798562 25.714590298824508 25.440671782634034 24.5213138107429 94-95 24.369968499802916 25.42876755537967 25.746352154975366 24.45491178984205 96-97 24.561729237856188 25.159953493843506 25.528261088321543 24.750056179978763 98-99 24.050595834262452 25.505945744373935 25.35111324441488 25.092345176948726 100-101 24.078679910478918 25.440692282932474 25.508878725137148 24.97174908145146 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 76.0 1 62.0 2 132.5 3 250.5 4 199.0 5 151.0 6 384.0 7 721.5 8 1026.5 9 1176.5 10 1026.0 11 887.0 12 911.5 13 1025.5 14 1166.5 15 1332.0 16 1449.0 17 1529.5 18 1707.5 19 1932.5 20 2175.5 21 2629.0 22 3323.5 23 4108.0 24 5238.0 25 6822.0 26 8760.0 27 11219.5 28 14007.5 29 17096.5 30 20602.5 31 25618.0 32 34539.0 33 37319.5 34 35681.0 35 38795.0 36 43678.0 37 48770.0 38 53889.5 39 59064.5 40 63711.5 41 69536.5 42 74015.5 43 78790.5 44 85470.0 45 93840.5 46 101071.0 47 106205.0 48 111686.5 49 116147.0 50 118968.5 51 122165.5 52 128344.0 53 137269.5 54 146722.0 55 144325.5 56 134364.5 57 127641.0 58 123008.0 59 113833.0 60 97857.5 61 84321.5 62 73114.5 63 57119.5 64 42645.5 65 33326.5 66 24126.5 67 19419.5 68 15033.0 69 10461.0 70 8173.0 71 5507.5 72 4088.5 73 3540.5 74 2761.5 75 2071.0 76 1528.0 77 989.0 78 615.0 79 349.0 80 225.5 81 151.5 82 77.5 83 38.0 84 21.0 85 7.5 86 4.5 87 2.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26754734202187685 2 0.01936923076438543 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.0943089420652134E-4 12-13 7.873671042433103E-5 14-15 0.0 16-17 5.196622888005848E-4 18-19 0.0 20-21 6.298936833946482E-5 22-23 4.724202625459861E-5 24-25 0.0022203752339661352 26-27 0.002299111944390466 28-29 0.0024250906810693957 30-31 0.001307029393043895 32-33 0.0015274921822320219 34-35 0.0015274921822320219 36-37 9.448405250919722E-5 38-39 1.1023139459406345E-4 40-41 8.188617884130427E-4 42-43 0.0035274046270100303 44-45 0.004330519073338207 46-47 0.013259262035457346 48-49 0.013416735456306008 50-51 0.006078474044758355 52-53 0.006913083175256264 54-55 4.0943089420652134E-4 56-57 0.0025825641019180576 58-59 0.0015747342084866205 60-61 0.0014802501559774235 62-63 0.0 64-65 0.0010550719196860358 66-67 0.0 68-69 0.0 70-71 1.5747342084866206E-4 72-73 0.0013227767351287614 74-75 0.0 76-77 0.19317264535505377 78-79 0.10476706689061487 80-81 0.0011653033142800993 82-83 0.0 84-85 7.716197621584442E-4 86-87 0.12163247026350657 88-89 0.5828721199292377 90-91 0.3074196121807581 92-93 0.2908219136233091 94-95 0.002503827391493727 96-97 0.002377848654814797 98-99 0.002991994996124579 100-101 6.613883675643807E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3175139.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.696784192914656 #Duplication Level Percentage of deduplicated Percentage of total 1 66.72262975045608 27.15396463738058 2 15.755867701962858 12.824262952777932 3 6.514530789422368 7.953613609656602 4 3.3767904499414714 5.496980488238529 5 1.9474180442205729 3.962682593951629 6 1.2511926850412887 3.0551711212127257 7 0.8567986030477001 2.4408263491516102 8 0.6284886663704039 2.0461974098375255 9 0.4485886804301737 1.6430505046966022 >10 2.2379481894707203 16.294073712883197 >50 0.15385754667022097 4.313559785117548 >100 0.09461017450496559 7.318529587273452 >500 0.00672088017930125 1.7789687271478019 >1k 0.0044805867855669205 3.5494489577146857 >5k 7.725149630287794E-5 0.16866956295960397 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5365 0.16896898057061438 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4881 0.15372555343246389 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4607 0.14509600996995722 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3990 0.12566378983723234 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3740 0.11779011879479921 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3663 0.11536502811372984 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3567 0.11234153843343551 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3312 0.10431039397015374 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3256 0.10254669165664873 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.298936833946482E-5 2 0.0 0.0 0.0 0.0 6.298936833946482E-5 3 0.0 0.0 0.0 0.0 6.298936833946482E-5 4 0.0 0.0 0.0 6.298936833946482E-5 9.448405250919724E-5 5 0.0 0.0 0.0 6.298936833946482E-5 9.448405250919724E-5 6 0.0 0.0 0.0 6.298936833946482E-5 1.2597873667892964E-4 7 0.0 0.0 0.0 9.448405250919724E-5 1.2597873667892964E-4 8 0.0 0.0 0.0 9.448405250919724E-5 1.2597873667892964E-4 9 0.0 0.0 0.0 1.5747342084866206E-4 1.2597873667892964E-4 10-11 0.0 0.0 0.0 2.204627891881269E-4 1.8896810501839448E-4 12-13 0.0 0.0 0.0 3.149468416973241E-4 5.511569729703173E-4 14-15 0.0 0.0 0.0 3.306941837821903E-4 7.401250779887117E-4 16-17 0.0 0.0 0.0 4.2517823629138757E-4 8.188617884130427E-4 18-19 0.0 0.0 0.0 4.724202625459862E-4 0.0010078298934314374 20-21 0.0 0.0 0.0 5.196622888005848E-4 0.0011023139459406344 22-23 0.0 0.0 0.0 6.613883675643806E-4 0.001149555972195233 24-25 0.0 0.0 0.0 8.50356472582775E-4 0.0011967979984498316 26-27 0.0 0.0 0.0 0.0011338086301103667 0.0013385240772136274 28-29 0.0 0.0 0.0 0.0015747342084866207 0.001385766103468226 30-31 0.0 0.0 0.0 0.0030234896802943116 0.001385766103468226 32-33 0.0 0.0 0.0 0.005842263913485362 0.001448755471807691 34-35 0.0 0.0 0.0 0.010314509065587365 0.0014802501559774233 36-37 0.0 0.0 0.0 0.017637023135050153 0.0014802501559774233 38-39 0.0 0.0 0.0 0.031541926195987016 0.0016062288926563528 40-41 0.0 0.0 0.0 0.04604522825614878 0.0016377235768260854 42-43 0.0 0.0 0.0 0.061225666025959816 0.0016534709189109514 44-45 0.0 0.0 0.0 0.07979178234401707 0.00170071294516555 46-47 0.0 0.0 0.0 0.10105069415858645 0.0018109443397596138 48-49 0.0 0.0 0.0 0.12601023136309938 0.001905428392268811 50-51 0.0 0.0 0.0 0.15607190740310897 0.0019526704185234096 52-53 0.0 0.0 0.0 0.184669080629226 0.0021888805497964027 54-55 0.0 0.0 0.0 0.21449454653796257 0.002220375233966135 56-57 0.0 0.0 0.0 0.25151654777948307 0.0022676172602207334 58-59 0.0 0.0 0.0 0.29249113188430487 0.002299111944390466 60-61 0.0 0.0 0.0 0.3400008629543463 0.002299111944390466 62-63 0.0 0.0 0.0 0.38549493423752473 0.0024250906810693957 64-65 0.0 0.0 0.0 0.4358076921986722 0.002519574733578593 66-67 0.0 0.0 0.0 0.49408860525476206 0.002598311444002924 68-69 0.0 0.0 0.0 0.5642587615849259 0.0026455534702575227 70-71 0.0 0.0 0.0 0.6426017884571352 0.002661300812342389 72-73 0.0 0.0 0.0 0.7244092305880152 0.0028502689173607832 74-75 0.0 0.0 0.0 0.8058859785351129 0.002991994996124579 76-77 0.0 0.0 0.0 0.8960237646288871 0.0030234896802943116 78-79 0.0 0.0 0.0 1.0045701936198699 0.003102226390718643 80-81 0.0 0.0 0.0 1.1252105813320297 0.003212457785312706 82-83 0.0 0.0 0.0 1.2537403874287079 0.0034014258903311005 84-85 0.0 0.0 0.0 1.3801757970280986 0.003464415258670565 86-87 0.0 0.0 0.0 1.516327316693852 0.0036376360216040936 88-89 0.0 0.0 0.0 1.6690607875749692 0.003810856784537622 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3820 0.0 44.832634 1 GTATCAA 6685 0.0 42.1996 1 ATCAACG 8585 0.0 32.32495 3 TCAACGC 8605 0.0 32.304947 4 CAACGCA 8845 0.0 31.374754 5 TATCAAC 8980 0.0 31.11929 2 AACGCAG 9270 0.0 30.038668 6 TACATGG 12365 0.0 28.35574 2 GTACATG 13070 0.0 27.444216 1 ACATGGG 12240 0.0 27.439367 3 ACGCAGA 11355 0.0 24.314095 7 CGCAGAG 11500 0.0 24.007526 8 TAAGGTG 1565 0.0 22.430536 5 GAGTACA 9290 0.0 21.763477 1 CATGGGA 4930 0.0 21.74622 4 GTAAGGT 1420 0.0 21.714373 4 CATGGGG 7870 0.0 21.578962 4 AGTACAT 9305 0.0 20.293518 2 AGGTAAG 1360 0.0 20.233908 2 GCAGAGT 14230 0.0 19.268381 9 >>END_MODULE