FastQCFastQC Report
Thu 26 May 2016
SRR936353_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936353_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202618
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75440.34250151410730323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53090.24103135450631927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36550.1659388963497075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47250.048.1995241
GTATCAA80100.040.5500221
ATCAACG101700.031.4599153
GTACATG140850.031.1617851
TCAACGC103950.030.8244954
CAACGCA106150.030.1856485
TATCAAC107350.029.9804842
TACATGG144450.029.9474932
AACGCAG107700.029.7491956
ACATGGG145850.028.3295463
AGTACAT96850.026.3403132
GAGTACA105150.026.296811
ACGCAGA128950.024.5903997
CATGGGG64250.024.014654
CGCAGAG132400.023.950188
CATGGGA81250.023.8831884
TAGATCG1050.002243247822.5378515
GCAGAGT144050.021.4541859
TGCACCG6950.021.1109965
ATGGGAT30950.020.0328375
Produced by FastQC (version 0.11.5)