##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936353_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2202618 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87438402846068 33.0 31.0 34.0 30.0 34.0 2 31.951010116143607 34.0 31.0 34.0 30.0 34.0 3 32.02541384842946 34.0 31.0 34.0 30.0 34.0 4 35.5219416167488 37.0 35.0 37.0 33.0 37.0 5 35.51920124143179 37.0 35.0 37.0 33.0 37.0 6 35.61702346934421 37.0 35.0 37.0 33.0 37.0 7 35.56803994156045 37.0 35.0 37.0 33.0 37.0 8 35.570131997468465 37.0 35.0 37.0 33.0 37.0 9 37.247808289953134 39.0 37.0 39.0 34.0 39.0 10-11 37.17924556141827 39.0 37.0 39.0 34.0 39.0 12-13 37.09042671039644 39.0 37.0 39.0 34.0 39.0 14-15 38.34160008680579 40.0 38.0 41.0 33.5 41.0 16-17 38.303766018438054 40.0 38.0 41.0 33.0 41.0 18-19 38.272162036267744 40.0 38.0 41.0 33.5 41.0 20-21 38.20664363952351 40.0 38.0 41.0 33.5 41.0 22-23 38.05152414081788 40.0 38.0 41.0 33.0 41.0 24-25 38.192310241721444 40.0 38.0 41.0 34.0 41.0 26-27 38.13783960723103 40.0 38.0 41.0 33.5 41.0 28-29 38.00857865503687 40.0 38.0 41.0 33.0 41.0 30-31 37.84347195019745 40.0 38.0 41.0 33.0 41.0 32-33 37.652216135526 40.0 37.0 41.0 33.0 41.0 34-35 37.38223377816762 40.0 37.0 41.0 32.0 41.0 36-37 37.17618102639677 40.0 37.0 41.0 31.5 41.0 38-39 37.0086594679604 40.0 37.0 41.0 31.0 41.0 40-41 36.81083737624954 40.0 36.0 41.0 31.0 41.0 42-43 36.59877019074574 40.0 36.0 41.0 30.0 41.0 44-45 36.37547477592574 40.0 36.0 41.0 30.0 41.0 46-47 36.058997066218474 39.5 35.0 41.0 29.5 41.0 48-49 35.85585811974659 39.0 35.0 41.0 28.0 41.0 50-51 35.52458823999441 39.0 35.0 40.5 28.0 41.0 52-53 35.5329162841673 39.0 35.0 40.5 28.0 41.0 54-55 35.59961191636498 39.0 35.0 41.0 28.0 41.0 56-57 35.435673366875236 39.0 35.0 41.0 28.0 41.0 58-59 35.206079084071774 39.0 35.0 41.0 27.5 41.0 60-61 34.913410314453074 38.0 35.0 40.5 26.5 41.0 62-63 34.60266283123084 37.5 34.0 40.0 26.0 41.0 64-65 34.270185751682774 37.0 34.0 40.0 26.0 41.0 66-67 33.91714132909111 36.5 34.0 39.5 26.0 41.0 68-69 33.546736429103916 36.0 34.0 39.0 25.0 41.0 70-71 33.158508193431636 35.5 33.5 39.0 24.0 40.5 72-73 32.76565841194433 35.0 33.0 38.0 23.5 40.0 74-75 32.37063939366699 35.0 33.0 37.0 22.5 39.5 76-77 31.988112328147686 35.0 33.0 37.0 21.5 39.0 78-79 31.616558114026127 35.0 32.0 36.0 20.0 39.0 80-81 31.25561218513605 35.0 32.0 36.0 18.5 37.5 82-83 30.932648103302526 35.0 32.0 35.0 17.5 37.0 84-85 30.63753746677817 35.0 31.5 35.0 14.5 36.5 86-87 30.331136856231993 34.5 31.0 35.0 8.5 36.0 88-89 30.032967586753582 34.0 31.0 35.0 4.5 36.0 90-91 29.707138278176245 34.0 31.0 35.0 2.0 35.5 92-93 29.425892279096963 34.0 30.0 35.0 2.0 35.0 94-95 29.075015277274588 34.0 30.0 35.0 2.0 35.0 96-97 28.680942405809812 34.0 30.0 35.0 2.0 35.0 98-99 28.22880227075235 34.0 29.0 35.0 2.0 35.0 100-101 25.066176704267377 30.5 23.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2031888708869758 1101 2 0.13230474334239872 1101 3 0.2617871608841469 1101 4 0.10103712525415887 1101 5 0.28659680140825117 1101 6 0.13794154408238768 1101 7 0.18369488260712785 1101 8 0.1764570146334279 1101 9 0.16179576576981702 1101 10-11 0.3115650839023729 1101 12-13 0.26942550897584283 1101 14-15 0.34004581209575946 1101 16-17 0.32783580682620794 1101 18-19 0.4082618704267915 1101 20-21 0.3839706125637292 1101 22-23 0.3767811367486118 1101 24-25 0.3388733576748493 1101 26-27 0.26018613599233475 1101 28-29 0.23119611557294206 1101 30-31 0.2586161832632996 1101 32-33 0.2637560321327044 1101 34-35 0.15852239640533128 1101 36-37 -0.018734840622791182 1101 38-39 0.12228035257714964 1101 40-41 0.10312547854603338 1101 42-43 0.03857362494092342 1101 44-45 0.014048654486458645 1101 46-47 -0.11818127714959559 1101 48-49 -0.06804972124380271 1101 50-51 0.01741956907953579 1101 52-53 0.07146376178210545 1101 54-55 -0.06803575754935309 1101 56-57 -0.059883670634441444 1101 58-59 -0.10097156957098719 1101 60-61 -0.29049565971541114 1101 62-63 -0.3547536294710909 1101 64-65 -0.22924547161446895 1101 66-67 -0.13037899274667808 1101 68-69 -0.15830647853972124 1101 70-71 -0.04065607218321077 1101 72-73 0.08591162010303321 1101 74-75 -0.12015262270836047 1101 76-77 -0.2467931246259063 1101 78-79 -0.12837339759632016 1101 80-81 -0.11593874259773074 1101 82-83 0.01674901238743587 1101 84-85 -0.01625448683294195 1101 86-87 -0.05666171389033536 1101 88-89 -0.14715697899219293 1101 90-91 -0.16140998958610453 1101 92-93 -0.04612306753844919 1101 94-95 0.054240569007966144 1101 96-97 0.04866024832662674 1101 98-99 0.27212804713523653 1101 100-101 0.2768574403203026 1102 1 0.2621930001478816 1102 2 0.15980110725232421 1102 3 0.13845683672880682 1102 4 0.09164681815082787 1102 5 0.10036713425677846 1102 6 0.08709395894772598 1102 7 0.16545759220473855 1102 8 0.11899592497663747 1102 9 0.18814279086301866 1102 10-11 0.2014266617146987 1102 12-13 0.285121297798419 1102 14-15 0.3038082040579084 1102 16-17 0.2441050112329819 1102 18-19 0.2756988030713572 1102 20-21 0.19065809271150158 1102 22-23 0.2384404476468589 1102 24-25 0.3989662242512395 1102 26-27 0.2857691642284479 1102 28-29 0.3892343822864177 1102 30-31 0.3801267906157193 1102 32-33 0.29593208576294217 1102 34-35 0.13081079268064144 1102 36-37 0.24813703526533715 1102 38-39 0.10986164217710126 1102 40-41 0.13927949830629416 1102 42-43 0.023483433189419145 1102 44-45 -0.00955124186408085 1102 46-47 0.29283941930793134 1102 48-49 0.24848195035456655 1102 50-51 0.03297994489243905 1102 52-53 0.06736292088402962 1102 54-55 0.14547169016617545 1102 56-57 0.2792373150385288 1102 58-59 0.327750616429654 1102 60-61 0.26536220677738953 1102 62-63 0.4518439932782954 1102 64-65 0.14696530458628132 1102 66-67 0.21107243410521903 1102 68-69 0.15785113551648777 1102 70-71 0.27138440126079644 1102 72-73 0.33758505742018485 1102 74-75 0.30217756770574056 1102 76-77 0.36129445917134007 1102 78-79 0.40449978226893535 1102 80-81 0.3137731286094514 1102 82-83 0.4245197966126568 1102 84-85 0.39618219504679075 1102 86-87 0.2173327191303791 1102 88-89 0.13469558800381165 1102 90-91 0.26154836673400794 1102 92-93 0.12880379271866715 1102 94-95 0.3141712743258509 1102 96-97 0.4468902665488699 1102 98-99 0.3829238911260724 1102 100-101 0.5017291323142175 1103 1 0.3002481710493541 1103 2 0.2896743135347499 1103 3 0.3787611849668622 1103 4 0.2560785205057243 1103 5 0.38792017929581846 1103 6 0.21676087332711091 1103 7 0.28949777310270264 1103 8 0.2790261919513881 1103 9 0.43632386209193186 1103 10-11 0.44816585541656906 1103 12-13 0.5332096459606745 1103 14-15 0.5227788020472488 1103 16-17 0.5039601195971457 1103 18-19 0.3908917144267505 1103 20-21 0.5162351753595686 1103 22-23 0.6028759401417005 1103 24-25 0.5656404711978027 1103 26-27 0.5492148887475352 1103 28-29 0.5363229456744776 1103 30-31 0.502182427162964 1103 32-33 0.5599260269535051 1103 34-35 0.5357791247077301 1103 36-37 0.4273345814204461 1103 38-39 0.5999033201803456 1103 40-41 0.6020826587415655 1103 42-43 0.5632654189249564 1103 44-45 0.6786848405389776 1103 46-47 0.6899597251180793 1103 48-49 0.8349097299146138 1103 50-51 0.6614761121134123 1103 52-53 0.748625145777396 1103 54-55 0.6800607780825345 1103 56-57 0.5724968218601063 1103 58-59 0.6283161594551814 1103 60-61 0.48556730749358934 1103 62-63 0.6468737661812298 1103 64-65 0.5917590735962008 1103 66-67 0.6621766507712792 1103 68-69 0.6934436629848477 1103 70-71 0.7839231317534185 1103 72-73 0.7794474239023472 1103 74-75 0.7146946402557859 1103 76-77 0.5906138989345138 1103 78-79 0.6394938737114728 1103 80-81 0.6812090583240114 1103 82-83 0.7135903866156212 1103 84-85 0.7878800288464483 1103 86-87 0.9646125492238227 1103 88-89 0.8286676411361604 1103 90-91 0.7585281666416854 1103 92-93 0.659099599605625 1103 94-95 0.5624121756211302 1103 96-97 0.7990482774834256 1103 98-99 0.9435746581556472 1103 100-101 1.0274021867203231 1104 1 0.24609759740180692 1104 2 0.14817125685728527 1104 3 0.21180057248063378 1104 4 0.1686645597563583 1104 5 0.17358625491814195 1104 6 0.16114928430408781 1104 7 0.3105507393107132 1104 8 0.19533951498764424 1104 9 0.260674924040714 1104 10-11 0.2065925722672759 1104 12-13 0.2256911310521943 1104 14-15 0.39433398079633974 1104 16-17 0.36778762782898156 1104 18-19 0.22592242832757137 1104 20-21 0.40090126595723063 1104 22-23 0.35989536894012275 1104 24-25 0.5041251820305206 1104 26-27 0.39048033666658455 1104 28-29 0.3709231841764975 1104 30-31 0.28956344216010876 1104 32-33 0.40925333196732794 1104 34-35 0.38897729288186156 1104 36-37 0.41919745352813464 1104 38-39 0.2956676892179644 1104 40-41 0.32442182291256216 1104 42-43 0.3837207901728732 1104 44-45 0.32271938681370216 1104 46-47 0.29775302441447593 1104 48-49 0.3240841340602074 1104 50-51 0.25589708789092214 1104 52-53 0.16731665820023522 1104 54-55 0.2666391410254505 1104 56-57 0.23856653286490115 1104 58-59 0.12993218275576623 1104 60-61 0.17517470920083156 1104 62-63 0.23939064037022462 1104 64-65 0.3465457314598197 1104 66-67 0.31824756243068464 1104 68-69 0.23187958572994916 1104 70-71 0.1833458454587742 1104 72-73 0.3179081741879983 1104 74-75 0.23303812047795702 1104 76-77 0.29202357778699195 1104 78-79 0.33154580294274183 1104 80-81 0.42128533273217883 1104 82-83 0.49401811390781347 1104 84-85 0.22485262423346342 1104 86-87 0.2663810582866617 1104 88-89 0.4191264567127213 1104 90-91 0.5261095464591818 1104 92-93 0.40841973221884587 1104 94-95 0.17335541986344083 1104 96-97 0.45984154499005925 1104 98-99 0.49486100061754357 1104 100-101 0.7894975996154585 1105 1 0.10923351681435634 1105 2 0.04372664924902736 1105 3 0.06582791385827846 1105 4 -0.02738458821312406 1105 5 -0.06517127448225324 1105 6 0.01143554849271311 1105 7 0.18090766688893467 1105 8 0.09431932901621565 1105 9 0.0924261150314507 1105 10-11 0.16831055605702971 1105 12-13 0.0506129070381931 1105 14-15 0.0904902365601643 1105 16-17 0.002545885915623103 1105 18-19 0.06930560147861087 1105 20-21 0.0650209370290753 1105 22-23 0.06462125751219361 1105 24-25 0.10583444076132054 1105 26-27 0.010671177776906404 1105 28-29 0.09603941947251826 1105 30-31 0.2964352461152515 1105 32-33 0.22012001695207317 1105 34-35 -0.03004883612133824 1105 36-37 -0.03093383867631161 1105 38-39 0.016422151979647026 1105 40-41 0.010456076716906182 1105 42-43 0.20707240878738276 1105 44-45 0.13698072024392616 1105 46-47 0.0531729437392201 1105 48-49 0.10765094724630586 1105 50-51 0.19886817988832206 1105 52-53 0.20692370758361278 1105 54-55 0.30958583414484053 1105 56-57 0.36240080518918205 1105 58-59 0.35255630100882485 1105 60-61 0.2526951423149839 1105 62-63 0.026947439671417328 1105 64-65 0.03083156721726965 1105 66-67 0.135624231400989 1105 68-69 0.003910422682118053 1105 70-71 0.07444118855317328 1105 72-73 0.051856954710878256 1105 74-75 0.13182895043593845 1105 76-77 0.10739976584456201 1105 78-79 0.07947726000992361 1105 80-81 0.1700974959387196 1105 82-83 0.09922622725385111 1105 84-85 -0.021799342550753664 1105 86-87 -0.1012260122911961 1105 88-89 0.0446502314891255 1105 90-91 0.0920468204005509 1105 92-93 0.08722477761413217 1105 94-95 0.1366759835254605 1105 96-97 0.0743284229605976 1105 98-99 0.010893611850061546 1105 100-101 0.07758098069944097 1106 1 0.12354714594815164 1106 2 0.15287346293389348 1106 3 0.12239934049117807 1106 4 0.11678203241343255 1106 5 0.08631717817252138 1106 6 0.058933437916962816 1106 7 0.19027101449955097 1106 8 0.1341697131846189 1106 9 0.23922501859824763 1106 10-11 0.21166313900435796 1106 12-13 0.22489509319728285 1106 14-15 0.21169750043011248 1106 16-17 0.16392642054312034 1106 18-19 0.16885680729343022 1106 20-21 0.2932950918537358 1106 22-23 0.2849038178077805 1106 24-25 0.15618548451494263 1106 26-27 0.18318275807715878 1106 28-29 0.250009918905981 1106 30-31 0.2807118202267418 1106 32-33 0.25458310198865064 1106 34-35 0.08662795937041778 1106 36-37 0.0890847421444434 1106 38-39 0.19949303243140548 1106 40-41 0.1025604152464723 1106 42-43 0.13062025512777353 1106 44-45 0.1642152650622819 1106 46-47 0.10097603065617022 1106 48-49 0.16888890291016168 1106 50-51 0.16078464567470263 1106 52-53 0.1259918508294362 1106 54-55 0.21093568182475053 1106 56-57 0.15749067549175777 1106 58-59 0.16256450099545106 1106 60-61 0.3691580935929082 1106 62-63 0.3561063885610736 1106 64-65 0.3005174718667405 1106 66-67 0.24319744245178043 1106 68-69 0.2677917593768555 1106 70-71 0.3935633925855129 1106 72-73 0.44107571697815473 1106 74-75 0.3348199343277116 1106 76-77 0.3756464322979909 1106 78-79 0.3134131372776423 1106 80-81 0.2843426464608143 1106 82-83 0.2333302963275905 1106 84-85 0.24728573861069592 1106 86-87 0.39566107020978336 1106 88-89 0.23996123972885286 1106 90-91 0.21673992669311914 1106 92-93 0.13347825121957513 1106 94-95 0.2380393524599178 1106 96-97 0.2373311446582882 1106 98-99 0.2898704115096571 1106 100-101 0.6475566230559586 1107 1 0.01398435904535944 1107 2 0.0638136861753118 1107 3 0.15608195557037163 1107 4 0.17394044011681586 1107 5 0.06281563624356323 1107 6 0.19657609411426336 1107 7 0.14703288290949956 1107 8 0.14068075307281447 1107 9 0.13536494536924693 1107 10-11 0.1776164994400986 1107 12-13 0.11611639786911354 1107 14-15 0.20694927814192 1107 16-17 0.05162108023828438 1107 18-19 0.053803107370782755 1107 20-21 0.2506456600550635 1107 22-23 0.23018654517414205 1107 24-25 0.2750435179950941 1107 26-27 0.1737009552623121 1107 28-29 0.24432689587701617 1107 30-31 0.1463575017149168 1107 32-33 0.14211493519778884 1107 34-35 0.11136546792828028 1107 36-37 0.30512898247936704 1107 38-39 0.2620166359607765 1107 40-41 0.35413692905315486 1107 42-43 0.02132884570171001 1107 44-45 0.2533055875647534 1107 46-47 0.24068645700407387 1107 48-49 0.2715766254396854 1107 50-51 0.20650001185001088 1107 52-53 0.27435202402632797 1107 54-55 0.35413513896882165 1107 56-57 0.32976387460558243 1107 58-59 0.4468969073538176 1107 60-61 0.4272447384887883 1107 62-63 0.5515734001674488 1107 64-65 0.3471416370338929 1107 66-67 0.2659699256339465 1107 68-69 0.36182064706748207 1107 70-71 0.39796753347763314 1107 72-73 0.3576853312636601 1107 74-75 0.34068345493422925 1107 76-77 0.41241542973113354 1107 78-79 0.3424522943968711 1107 80-81 0.4941762554631772 1107 82-83 0.6068979281387854 1107 84-85 0.6196701646233471 1107 86-87 0.34432377249328994 1107 88-89 0.5045992146015692 1107 90-91 0.6427137048128735 1107 92-93 0.3348840117934522 1107 94-95 0.3739386292578786 1107 96-97 0.4100274401916373 1107 98-99 0.392627051296067 1107 100-101 0.5729174667445491 1108 1 0.006104070087673108 1108 2 0.03957013612371085 1108 3 0.01628422595221224 1108 4 -0.05255697990382657 1108 5 0.020759908689385043 1108 6 -0.0858428945338261 1108 7 -0.10389797364364739 1108 8 -0.04704267313977084 1108 9 0.01188094124128014 1108 10-11 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2316 74-75 -0.9535346190508136 2316 76-77 -1.437529214732507 2316 78-79 -1.4221749813058935 2316 80-81 -1.1894269696451651 2316 82-83 -1.447274305381093 2316 84-85 -1.516377110256684 2316 86-87 -1.5124452527305934 2316 88-89 -1.5033248544148883 2316 90-91 -1.5214676860687533 2316 92-93 -1.7121310817565103 2316 94-95 -1.7093038458768461 2316 96-97 -1.9338550129081824 2316 98-99 -1.842666887496268 2316 100-101 -1.7699453355869998 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13442.0 3 3089.0 4 2596.0 5 2386.0 6 2391.0 7 2535.0 8 2986.0 9 3654.0 10 4418.0 11 5478.0 12 7411.0 13 11477.0 14 11790.0 15 10732.0 16 13110.0 17 16948.0 18 15054.0 19 9798.0 20 9254.0 21 9494.0 22 10175.0 23 11193.0 24 12835.0 25 14880.0 26 17680.0 27 21409.0 28 25917.0 29 31686.0 30 38586.0 31 49043.0 32 61982.0 33 82522.0 34 117727.0 35 180308.0 36 297428.0 37 491205.0 38 485657.0 39 94285.0 40 57.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.52971676580204 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23.227850917620735 26.17117084391684 28.147762865261544 22.45321537320088 20-21 24.012920578261486 25.3976898416505 28.11456173414882 22.4748278459392 22-23 23.589649868608497 25.33759386120744 28.021295071825463 23.0514611983586 24-25 23.127580313700754 25.655132609080162 28.205253957474362 23.01203311974472 26-27 23.11046808603379 25.882170522925286 28.051335861139275 22.95602552990165 28-29 22.979705498712082 25.897064182481362 28.255435763595226 22.86779455521133 30-31 23.029213801508526 25.819434486334853 28.144115124273423 23.0072365878832 32-33 22.882309858208423 25.88067476208012 28.205657950240585 23.031357429470873 34-35 23.266011176429483 25.80398160584865 28.065306839673482 22.864700378048383 36-37 23.373407849976484 25.785018176775242 27.832249052613427 23.00932492063485 38-39 23.23119501422799 25.866931124490115 27.971888887571062 22.929984973710837 40-41 23.24364007351398 25.85643876968114 27.91929705451313 22.980624102291745 42-43 23.230705254295167 25.849629421625824 27.658347519961456 23.26131780411755 44-45 23.169698091073005 26.091117720777078 27.624925766738617 23.1142584214113 46-47 23.198642904567535 26.244327373034686 27.356447458183986 23.200582264213796 48-49 23.514505096675165 26.233918371510008 27.068909220472133 23.1826673113427 50-51 23.34358251611428 26.471028525345393 26.87140386724471 23.31398509129562 52-53 23.58091715102118 26.483690070612738 26.760600980642472 23.174791797723607 54-55 23.31977467485342 26.34691685962032 26.866494325919305 23.466814139606957 56-57 23.257218311224754 26.610622977866782 26.800363008701535 23.331795702206932 58-59 23.366514846132 26.295658799602595 26.918316902267954 23.41950945199745 60-61 23.464026657414042 26.38020029560037 27.03146553956193 23.12430750742366 62-63 23.362242748294644 26.43976209100409 26.976934744289803 23.22106041641146 64-65 23.270313403703625 26.323994611003183 26.891550431724355 23.51414155356884 66-67 23.249454975427707 26.5740022252604 26.80186560576761 23.374677193544283 68-69 23.204130723499482 26.340047287666945 27.036288938003327 23.41953305083025 70-71 23.17708772927121 26.2398540803352 27.0391410886764 23.54391710171719 72-73 23.28841875498468 26.57114334156433 26.827403732965404 23.31303417048559 74-75 23.114921144269413 26.7109441559692 26.630470743225697 23.54366395653569 76-77 23.236596214924127 26.564381376576225 26.71022620206204 23.488796206437613 78-79 23.245864278291894 26.403054211803244 26.874006919199584 23.47707459070528 80-81 23.122562918119524 26.75082061161002 26.709482045100103 23.41713442517035 82-83 23.251827381984434 26.730811348475587 26.55582982761925 23.461531441920734 84-85 23.310247781275173 26.590613700621525 26.3474613991729 23.751677118930402 86-87 23.149843606339704 26.460141860799375 26.73888989019363 23.65112464266729 88-89 23.099543902858084 26.498128174043877 26.65958414363649 23.74274377946155 90-91 23.178060681860714 26.567462581649377 26.65348256549067 23.600994170999236 92-93 23.146119877070927 26.453005096485903 26.672651703921396 23.728223322521778 94-95 23.103129118604635 26.48568627688065 26.77022004598807 23.640964558526647 96-97 23.091717916290825 26.439788486253548 26.66530583852292 23.803187758932715 98-99 22.943552644691973 26.27159287682217 26.602269169133624 24.182585309352234 100-101 23.052340439216227 26.51025194414155 26.38031940551701 24.05708821112521 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 389.0 1 280.5 2 387.0 3 628.5 4 489.5 5 463.5 6 988.0 7 1815.5 8 2615.0 9 2866.0 10 2425.5 11 2110.5 12 2234.0 13 2456.5 14 2667.5 15 2935.0 16 3215.0 17 3333.0 18 3378.0 19 3518.0 20 3725.0 21 4027.0 22 4384.5 23 4926.0 24 5915.0 25 7273.0 26 8915.5 27 10835.0 28 13041.5 29 15823.0 30 19673.0 31 23523.0 32 27286.0 33 31186.0 34 34964.5 35 38979.0 36 44690.0 37 50124.0 38 52741.5 39 54844.0 40 56751.5 41 58947.5 42 61561.0 43 65011.0 44 69180.5 45 73433.5 46 76043.5 47 77091.5 48 80744.5 49 82534.5 50 81375.5 51 80473.5 52 80644.5 53 82412.5 54 82947.5 55 79031.0 56 72177.0 57 65796.5 58 61665.5 59 56861.5 60 50432.5 61 44103.5 62 37066.0 63 29574.5 64 22777.5 65 17723.5 66 13680.5 67 11005.0 68 8500.0 69 6165.0 70 4813.0 71 3757.0 72 2981.0 73 2599.0 74 2008.5 75 1558.5 76 1194.0 77 722.5 78 433.0 79 247.5 80 145.0 81 105.5 82 70.5 83 38.5 84 36.5 85 35.5 86 27.5 87 19.5 88 21.5 89 23.0 90 18.5 91 21.5 92 21.5 93 19.5 94 19.0 95 19.0 96 19.5 97 18.0 98 12.5 99 8.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.049168761900611 2 0.013983359801835816 3 0.011531731784630834 4 0.006855478344406521 5 0.010169716219516957 6 0.006310672118360968 7 0.0055388632981297715 8 0.008898501692077336 9 0.015390775885786823 10-11 0.0028829329461577085 12-13 0.00901200298917016 14-15 0.008399095984868914 16-17 0.01909091817101286 18-19 0.013256951500441747 20-21 0.003859044101155988 22-23 0.010464819591958297 24-25 0.02213275293310052 26-27 0.008217493909520398 28-29 0.01909091817101286 30-31 0.015299974848112565 32-33 0.013665556169975909 34-35 0.26974718267080355 36-37 0.47829446594915687 38-39 0.4748894270363722 40-41 0.49604606881447444 42-43 0.4860806549297245 44-45 0.5015168313343485 46-47 0.5072599969672453 48-49 0.514682981797116 50-51 0.5243305920500059 52-53 0.5130031626001422 54-55 0.5007450225141173 56-57 0.49645467348400857 58-59 0.49420734780157066 60-61 0.4906661073322746 62-63 0.489031688654138 64-65 0.5046494671341104 66-67 0.4773864555724143 68-69 0.5056482785485272 70-71 0.4870113655658857 72-73 0.49495645636238333 74-75 0.5089852166830562 76-77 0.49509265791889473 78-79 0.49443435039575634 80-81 0.49758968645493684 82-83 0.49749888541726256 84-85 0.49883820072295787 86-87 0.5009039243300473 88-89 0.5134117672696763 90-91 0.5379053471823076 92-93 0.5265779177324438 94-95 0.49770318775202965 96-97 0.46878305725277825 98-99 0.46042936178674654 100-101 0.46882845777161547 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2202618.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.88917548289507 #Duplication Level Percentage of deduplicated Percentage of total 1 65.10414748101255 25.96950763534391 2 14.97947674209999 11.950379528151437 3 6.594031260418014 7.890914102595296 4 3.660000686068144 5.839776385363541 5 2.299788403241479 4.586833159521319 6 1.5533497606429154 3.7177104713158977 7 1.0856638788911122 3.031436588636967 8 0.7985598175813905 2.548311415767421 9 0.6251178536638234 2.2441892186058277 >10 3.057534389819397 21.246324835625686 >50 0.167506195838261 4.490836549564885 >100 0.07186402898979256 4.915730601803226 >500 0.0020488858152257123 0.5375456239095188 >1k 6.829619384085662E-4 0.44691794713585303 >5k 2.2765397946952203E-4 0.5835859366592624 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7544 0.34250151410730323 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5309 0.24103135450631927 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3655 0.1659388963497075 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.540051883712927E-5 0.0 10-11 4.540051883712927E-5 0.0 0.0 6.810077825569391E-5 0.0 12-13 4.540051883712927E-5 0.0 0.0 9.080103767425854E-5 0.0 14-15 4.540051883712927E-5 0.0 0.0 9.080103767425854E-5 0.0 16-17 4.540051883712927E-5 0.0 0.0 1.5890181592995243E-4 0.0 18-19 4.540051883712927E-5 0.0 0.0 2.7240311302277566E-4 0.0 20-21 4.540051883712927E-5 0.0 0.0 4.313049289527281E-4 0.0 22-23 4.540051883712927E-5 0.0 0.0 9.98811414416844E-4 0.0 24-25 4.540051883712927E-5 0.0 0.0 0.0018160207534851707 0.0 26-27 4.540051883712927E-5 0.0 0.0 0.0023154264606935924 0.0 28-29 4.540051883712927E-5 0.0 0.0 0.0032234368374361782 0.0 30-31 4.540051883712927E-5 0.0 0.0 0.006015568745919629 0.0 32-33 4.540051883712927E-5 0.0 0.0 0.011282028931026623 0.0 34-35 4.540051883712927E-5 0.0 0.0 0.01797860545950319 0.0 36-37 4.540051883712927E-5 0.0 0.0 0.030577249436806564 0.0 38-39 4.540051883712927E-5 0.0 0.0 0.0518019919931645 0.0 40-41 4.540051883712927E-5 0.0 0.0 0.07577346593916875 0.0 42-43 4.540051883712927E-5 0.0 0.0 0.10144745934156535 0.0 44-45 4.540051883712927E-5 0.0 0.0 0.12802946312070454 0.0 46-47 4.540051883712927E-5 0.0 0.0 0.16314676444122403 0.0 48-49 4.540051883712927E-5 0.0 0.0 0.2030992210178978 0.0 50-51 4.540051883712927E-5 0.0 0.0 0.24679722039863472 0.0 52-53 4.540051883712927E-5 0.0 0.0 0.29015471588809316 0.0 54-55 4.540051883712927E-5 0.0 0.0 0.337870661185916 0.0 56-57 4.540051883712927E-5 0.0 0.0 0.3959833252974415 0.0 58-59 4.540051883712927E-5 0.0 0.0 0.46086066671569925 0.0 60-61 4.540051883712927E-5 0.0 0.0 0.5370200370649836 0.0 62-63 4.540051883712927E-5 0.0 0.0 0.6118173918491541 0.0 64-65 4.540051883712927E-5 0.0 0.0 0.6866147466333246 0.0 66-67 4.540051883712927E-5 0.0 0.0 0.7743058487672397 0.0 68-69 4.540051883712927E-5 0.0 0.0 0.8830173911227457 0.0 70-71 4.540051883712927E-5 0.0 0.0 1.0029882621498598 0.0 72-73 4.540051883712927E-5 0.0 0.0 1.1229818334363926 0.0 74-75 4.540051883712927E-5 0.0 0.0 1.2432705080953665 0.0 76-77 4.540051883712927E-5 0.0 0.0 1.3744099067564144 0.0 78-79 9.080103767425854E-5 0.0 0.0 1.538510082093218 0.0 80-81 9.080103767425854E-5 0.0 0.0 1.7273308399368388 0.0 82-83 9.080103767425854E-5 0.0 0.0 1.9175136133455735 0.0 84-85 9.080103767425854E-5 0.0 0.0 2.100772807631645 0.0 86-87 9.080103767425854E-5 4.540051883712927E-5 0.0 2.299445478062923 0.0 88-89 9.080103767425854E-5 4.540051883712927E-5 0.0 2.5292175038976343 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4725 0.0 48.199524 1 GTATCAA 8010 0.0 40.550022 1 ATCAACG 10170 0.0 31.459915 3 GTACATG 14085 0.0 31.161785 1 TCAACGC 10395 0.0 30.824495 4 CAACGCA 10615 0.0 30.185648 5 TATCAAC 10735 0.0 29.980484 2 TACATGG 14445 0.0 29.947493 2 AACGCAG 10770 0.0 29.749195 6 ACATGGG 14585 0.0 28.329546 3 AGTACAT 9685 0.0 26.340313 2 GAGTACA 10515 0.0 26.29681 1 ACGCAGA 12895 0.0 24.590399 7 CATGGGG 6425 0.0 24.01465 4 CGCAGAG 13240 0.0 23.95018 8 CATGGGA 8125 0.0 23.883188 4 TAGATCG 105 0.0022432478 22.537851 5 GCAGAGT 14405 0.0 21.454185 9 TGCACCG 695 0.0 21.110996 5 ATGGGAT 3095 0.0 20.032837 5 >>END_MODULE