FastQCFastQC Report
Thu 26 May 2016
SRR936353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936353_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202618
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80020.36329495173470844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59140.2684986684027825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40510.18391750180921068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49000.045.055391
GTATCAA87550.040.107451
ATCAACG109100.032.0445633
TCAACGC111550.031.3407614
GTACATG131850.031.1426451
TATCAAC113250.031.0470122
CAACGCA113500.030.802315
AACGCAG115550.030.3789986
TACATGG135600.029.7089862
ACATGGG134800.028.8911573
CATGGGA77550.025.9967354
ACGCAGA137950.025.308587
CGCAGAG140300.024.7832328
CATGGGG57100.023.7597354
AGTACAT90400.023.2527942
GCAGAGT152750.022.5458749
GAGTACA103450.022.444721
AACGTCG652.9694443E-421.89446422-23
GTGTAGC12750.021.6442551
ATGGGAG34000.021.067345
Produced by FastQC (version 0.11.5)