##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936353_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2202618 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49854445936608 34.0 31.0 34.0 31.0 34.0 2 32.61758053371034 34.0 31.0 34.0 31.0 34.0 3 32.70478040223043 34.0 31.0 34.0 31.0 34.0 4 36.1849408295038 37.0 35.0 37.0 35.0 37.0 5 36.155718331549096 37.0 35.0 37.0 35.0 37.0 6 36.1991480138635 37.0 37.0 37.0 35.0 37.0 7 36.14883924493489 37.0 36.0 37.0 35.0 37.0 8 36.1276235824823 37.0 36.0 37.0 35.0 37.0 9 37.8892504283539 39.0 38.0 39.0 35.0 39.0 10-11 37.830078343135305 39.0 38.0 39.0 35.0 39.0 12-13 37.7743893857219 39.0 38.0 39.0 35.0 39.0 14-15 39.111743616006045 40.5 38.5 41.0 36.0 41.0 16-17 39.0493719292224 40.0 38.0 41.0 36.0 41.0 18-19 39.09161030192253 40.0 38.5 41.0 36.0 41.0 20-21 39.055373196804894 40.0 39.0 41.0 35.5 41.0 22-23 38.93935421393996 40.0 38.0 41.0 35.0 41.0 24-25 39.07468135645854 40.0 39.0 41.0 35.0 41.0 26-27 38.987232693095216 40.0 38.5 41.0 35.0 41.0 28-29 38.88836466423138 40.0 38.0 41.0 35.0 41.0 30-31 38.75927578000362 40.0 38.0 41.0 35.0 41.0 32-33 38.58589278758278 40.0 38.0 41.0 34.5 41.0 34-35 38.43979913902456 40.0 38.0 41.0 34.0 41.0 36-37 38.2805114186845 40.0 38.0 41.0 34.0 41.0 38-39 38.13229098282135 40.0 38.0 41.0 33.5 41.0 40-41 37.96985269347658 40.0 37.5 41.0 33.0 41.0 42-43 37.96013153438318 40.0 38.0 41.0 33.0 41.0 44-45 37.78547301438561 40.0 37.0 41.0 33.0 41.0 46-47 37.52792086507965 40.0 37.0 41.0 33.0 41.0 48-49 37.261606188635525 40.0 37.0 41.0 32.0 41.0 50-51 37.0438439620488 40.0 36.0 41.0 32.0 41.0 52-53 36.82530152754586 40.0 36.0 41.0 31.0 41.0 54-55 36.62606657168878 39.0 35.5 41.0 31.0 41.0 56-57 36.37366125220079 39.0 35.0 41.0 31.0 41.0 58-59 36.11226186292857 39.0 35.0 41.0 30.0 41.0 60-61 35.83388585764758 38.0 35.0 40.5 30.0 41.0 62-63 35.53597627913692 38.0 35.0 40.0 29.0 41.0 64-65 35.16321758925061 37.0 35.0 40.0 29.0 41.0 66-67 34.815021261062974 36.5 34.0 39.5 29.0 41.0 68-69 34.4646123385898 36.0 34.0 39.0 28.0 41.0 70-71 34.10790840717728 35.5 34.0 39.0 28.0 40.5 72-73 33.71739516339193 35.0 34.0 38.0 27.5 40.0 74-75 33.30595613946676 35.0 33.5 37.0 26.5 39.5 76-77 32.59018177459732 35.0 32.5 36.0 26.0 39.0 78-79 32.64531684568091 35.0 33.0 36.0 26.0 39.0 80-81 32.49621518574714 35.0 33.0 36.0 26.0 37.5 82-83 32.23278480426474 35.0 33.0 35.5 26.0 37.0 84-85 31.960732637252576 35.0 33.0 35.0 25.5 37.0 86-87 31.57729256729946 35.0 32.5 35.0 24.5 36.0 88-89 31.107406050436346 35.0 32.0 35.0 22.0 36.0 90-91 30.910627943656138 35.0 32.0 35.0 20.5 36.0 92-93 30.650077090080984 34.0 32.0 35.0 19.5 35.0 94-95 30.511874051696662 34.0 31.5 35.0 19.0 35.0 96-97 30.291414580285824 34.0 31.0 35.0 16.5 35.0 98-99 29.888055713700695 34.0 31.0 35.0 2.0 35.0 100-101 26.69199062206883 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06740429755694777 1101 2 0.04930572747842632 1101 3 0.1540480801005799 1101 4 0.1177570684257816 1101 5 0.0925292539150746 1101 6 0.05114681484110406 1101 7 0.06014218275003458 1101 8 0.022027256454862254 1101 9 0.19783348065030282 1101 10-11 0.1299406586169667 1101 12-13 0.13443213665171072 1101 14-15 0.21688068473876143 1101 16-17 0.20523956317204295 1101 18-19 0.24282584576359056 1101 20-21 0.17853218056587394 1101 22-23 0.22727754399471678 1101 24-25 0.174222785888972 1101 26-27 0.08691335020954227 1101 28-29 0.20735399633374385 1101 30-31 0.12963940032506116 1101 32-33 0.2486530346012472 1101 34-35 0.15750236904037251 1101 36-37 0.22526559607761953 1101 38-39 0.13284461290978555 1101 40-41 0.12952457036340803 1101 42-43 -0.053764356942025415 1101 44-45 -0.006679282191896618 1101 46-47 0.029715964257555072 1101 48-49 -0.005477798218272767 1101 50-51 -0.16307158412339362 1101 52-53 -0.12966619576766192 1101 54-55 -0.09505151047655858 1101 56-57 -0.2047103554969425 1101 58-59 -0.31105436149454846 1101 60-61 -0.11764764926918048 1101 62-63 -0.28365579139289565 1101 64-65 -0.3298842573293044 1101 66-67 -0.35018959684554574 1101 68-69 -0.29983891235043103 1101 70-71 -0.2555212472170112 1101 72-73 -0.17772362441819212 1101 74-75 -0.2713500737971941 1101 76-77 -0.3460825525272284 1101 78-79 -0.35236316766560094 1101 80-81 -0.3134395371713552 1101 82-83 -0.352551884442434 1101 84-85 -0.1774719163424585 1101 86-87 -0.30635432336439905 1101 88-89 -0.2171710880234876 1101 90-91 0.08969655824973088 1101 92-93 0.07948354952360859 1101 94-95 -0.02048661050892875 1101 96-97 -0.21078507123637635 1101 98-99 -0.216088629425915 1101 100-101 0.08081495405042105 1102 1 0.03571848917471243 1102 2 0.14190314961594197 1102 3 0.10331417502342788 1102 4 0.0192215350489775 1102 5 0.07914008361232305 1102 6 0.0844299952078913 1102 7 0.09130389263184213 1102 8 0.07584473309194095 1102 9 0.13518155895580009 1102 10-11 0.12538300778192735 1102 12-13 0.22987323199249943 1102 14-15 0.19640448232138397 1102 16-17 0.2217269329832945 1102 18-19 0.28439638591104455 1102 20-21 0.1968254751142453 1102 22-23 0.21167997139829708 1102 24-25 0.18479335947173325 1102 26-27 0.24375714043640073 1102 28-29 0.21746901380368655 1102 30-31 0.3024535668663475 1102 32-33 0.295519728016167 1102 34-35 0.36410876855889995 1102 36-37 0.25772793955857054 1102 38-39 0.38845046074568046 1102 40-41 0.36391445952535406 1102 42-43 0.28460725982244384 1102 44-45 0.3760973532127494 1102 46-47 0.4612350140260233 1102 48-49 0.45627011625419556 1102 50-51 0.3986428950380514 1102 52-53 0.4048227455032176 1102 54-55 0.2621140946189868 1102 56-57 0.4827188034378054 1102 58-59 0.2609281016848186 1102 60-61 0.27769773749377435 1102 62-63 0.29842204601118283 1102 64-65 0.35051679922651857 1102 66-67 0.3088567444955501 1102 68-69 0.1566981088977073 1102 70-71 0.29684471897989084 1102 72-73 0.16650502295630076 1102 74-75 0.22832643956857623 1102 76-77 0.18832865164549872 1102 78-79 0.20490148953887655 1102 80-81 0.18075818254042986 1102 82-83 0.2507465089908649 1102 84-85 0.2995889526412725 1102 86-87 0.2506370001723859 1102 88-89 0.45558373084071135 1102 90-91 0.4826580073361093 1102 92-93 0.44038053533031984 1102 94-95 0.4774207569618589 1102 96-97 0.3530099201540331 1102 98-99 0.34428509376314764 1102 100-101 0.8228117944135249 1103 1 0.05122313954107227 1103 2 0.09662749138369264 1103 3 0.11648220411004218 1103 4 0.09064293228406939 1103 5 0.10417368726385234 1103 6 0.1028977228430179 1103 7 0.08946432113423697 1103 8 0.12253871583867948 1103 9 0.06778718541855966 1103 10-11 0.20102472585236342 1103 12-13 0.22001480725588607 1103 14-15 0.3122073354845938 1103 16-17 0.2135149179689293 1103 18-19 0.09096930075877907 1103 20-21 0.1559427244472502 1103 22-23 0.19837861060268835 1103 24-25 0.1428377284743405 1103 26-27 0.18887577858496485 1103 28-29 0.17117434937321008 1103 30-31 0.1595420084284811 1103 32-33 0.17376066992008532 1103 34-35 0.2239623879395154 1103 36-37 0.17651051953390606 1103 38-39 0.16832197500134072 1103 40-41 0.13812499367789854 1103 42-43 0.07484602510314176 1103 44-45 0.10402878958103656 1103 46-47 0.16657856990824627 1103 48-49 0.31753204335085883 1103 50-51 0.226614669324384 1103 52-53 0.1673592425205115 1103 54-55 0.1578163466389242 1103 56-57 0.20496056292905962 1103 58-59 0.2762012827898843 1103 60-61 0.2874289218177566 1103 62-63 0.33130787210603074 1103 64-65 0.3580874684163362 1103 66-67 0.1459198462648672 1103 68-69 0.20467166996430564 1103 70-71 0.13509846614235954 1103 72-73 0.05999234426136013 1103 74-75 0.1743781289557802 1103 76-77 0.2242760986478416 1103 78-79 0.20663642610140442 1103 80-81 0.32713528251484547 1103 82-83 0.46238927747152303 1103 84-85 0.3214733560800198 1103 86-87 0.35076559420643605 1103 88-89 0.4037033476575651 1103 90-91 0.5544313461380774 1103 92-93 0.6585493833578902 1103 94-95 0.5034517088690436 1103 96-97 0.28958625966393825 1103 98-99 0.34576595558682754 1103 100-101 0.7916801263052555 1104 1 -0.05817015681279969 1104 2 -0.005167526661594479 1104 3 -0.024328045959777 1104 4 -0.011760557548591066 1104 5 -0.07594473353642428 1104 6 0.05163082205308456 1104 7 -0.014969247066751734 1104 8 0.07241808551865603 1104 9 0.025216229227211784 1104 10-11 0.035032387447465396 1104 12-13 0.1491789440968816 1104 14-15 0.17702269083238065 1104 16-17 0.09269963933692793 1104 18-19 0.19220261211952305 1104 20-21 0.153438164192913 1104 22-23 0.11884640875715036 1104 24-25 0.0909032427052523 1104 26-27 0.19602006758069734 1104 28-29 0.2146013959441575 1104 30-31 0.12502226895739454 1104 32-33 0.00996839315055098 1104 34-35 0.07601218384137098 1104 36-37 0.07683924142526166 1104 38-39 0.13145951405874 1104 40-41 0.03337694329665197 1104 42-43 0.18624089413063416 1104 44-45 0.2652919412809851 1104 46-47 0.28475464157130403 1104 48-49 0.21206465110697792 1104 50-51 0.34324092446165366 1104 52-53 0.20117855635034232 1104 54-55 0.19734927886079134 1104 56-57 0.20373035172681853 1104 58-59 0.26421917017898977 1104 60-61 0.14104298413672467 1104 62-63 0.04079425203255482 1104 64-65 0.00711103671454083 1104 66-67 0.09323225843251493 1104 68-69 0.31979185233769414 1104 70-71 0.27325676706634283 1104 72-73 0.21373721750559582 1104 74-75 0.2637593767275348 1104 76-77 0.33024411394475095 1104 78-79 0.1896654228296839 1104 80-81 0.2603611524442826 1104 82-83 0.24154344388750815 1104 84-85 0.20500329113168902 1104 86-87 0.22080064438828018 1104 88-89 0.3318000316176857 1104 90-91 0.4929479780943531 1104 92-93 0.4977013376268715 1104 94-95 0.33116342145177313 1104 96-97 0.22493836542032852 1104 98-99 0.1312039856515348 1104 100-101 0.46583822965980914 1105 1 -0.04922104197230226 1105 2 0.07314454533300818 1105 3 -0.018750618883281334 1105 4 3.043974623508916E-4 1105 5 -0.051303380642607976 1105 6 -0.07018619039892116 1105 7 -0.07497862814518186 1105 8 0.009963518020938977 1105 9 0.0803894852309881 1105 10-11 -0.0017868315008016111 1105 12-13 -0.02705601886452058 1105 14-15 -0.006626175250580957 1105 16-17 0.06771602090005757 1105 18-19 0.035696047004670106 1105 20-21 -0.005880705929612873 1105 22-23 -0.0575594977393834 1105 24-25 0.03321680134090599 1105 26-27 0.058570741930864756 1105 28-29 -0.08960278052810366 1105 30-31 -0.009043510114935316 1105 32-33 -0.008217601994410018 1105 34-35 -0.02215986836667838 1105 36-37 -0.04043635008352453 1105 38-39 -0.1421634316856668 1105 40-41 0.008076416992416569 1105 42-43 -0.0049490419251299045 1105 44-45 -0.19131615546270808 1105 46-47 -0.07804809564471071 1105 48-49 -0.08247206920441386 1105 50-51 -0.12415743099845855 1105 52-53 -0.14915501693472777 1105 54-55 -0.1494262718990953 1105 56-57 -0.05344277963325794 1105 58-59 -0.08069303296904451 1105 60-61 -5.116356659229382E-4 1105 62-63 -0.03525682362491267 1105 64-65 -0.09381402643364112 1105 66-67 -0.07337309564516659 1105 68-69 -0.10227745006381639 1105 70-71 -0.05814118267095836 1105 72-73 -0.18674500726837095 1105 74-75 -0.12741254402429547 1105 76-77 -0.11439780918136933 1105 78-79 -0.34043981530916056 1105 80-81 -0.1647379506509381 1105 82-83 -0.05266829447072752 1105 84-85 -6.840444376550181E-4 1105 86-87 -0.018511599937593104 1105 88-89 0.03545799618607148 1105 90-91 0.043713414043256904 1105 92-93 0.06783663155009023 1105 94-95 -0.2720178440451093 1105 96-97 -0.3559541339305916 1105 98-99 -0.27902378683183215 1105 100-101 0.059671470014997396 1106 1 -0.04031712623637418 1106 2 -0.025181872290566787 1106 3 -0.025052056594880412 1106 4 -0.08191282658047783 1106 5 -0.06938527195111277 1106 6 -0.04065000335393165 1106 7 -0.02221059956347915 1106 8 0.012977933907592387 1106 9 -0.0012464217931764665 1106 10-11 0.015175679245935214 1106 12-13 0.04858146709324984 1106 14-15 0.08395629519409198 1106 16-17 0.01863883440658043 1106 18-19 0.07551153761296092 1106 20-21 0.07796597467509514 1106 22-23 0.015632773530910526 1106 24-25 0.07947185000288215 1106 26-27 0.061053394500525826 1106 28-29 0.07070683681553902 1106 30-31 0.035478976361389414 1106 32-33 0.1330409796405334 1106 34-35 0.17941623012620767 1106 36-37 0.08860597192781228 1106 38-39 0.11055317735815606 1106 40-41 -0.02079836746715813 1106 42-43 0.03447692508689215 1106 44-45 -0.008092979160089442 1106 46-47 -0.003750013530471108 1106 48-49 -0.17501548503194186 1106 50-51 -0.13864298361067284 1106 52-53 -0.1632085194242876 1106 54-55 -0.12841101953626577 1106 56-57 0.030179394836480355 1106 58-59 0.02325173246384793 1106 60-61 -0.07380798182554571 1106 62-63 -0.14632411585557037 1106 64-65 -0.08805586206752736 1106 66-67 -0.06894076540475425 1106 68-69 0.008018791415132398 1106 70-71 -0.0912857030735168 1106 72-73 -0.04161348277842336 1106 74-75 -0.015388344371103813 1106 76-77 -0.0020550846340441353 1106 78-79 -0.11858328890492231 1106 80-81 -0.1330051655342075 1106 82-83 -0.1286829458889116 1106 84-85 -0.04246229780173394 1106 86-87 -0.10699136991149061 1106 88-89 -0.08343078446815966 1106 90-91 0.08949037246854985 1106 92-93 0.02877535070442505 1106 94-95 0.09178544254054444 1106 96-97 0.04404237671339928 1106 98-99 0.013605966325311414 1106 100-101 0.28379317002924864 1107 1 0.05409850591410503 1107 2 0.06450295621906577 1107 3 0.05551751802660476 1107 4 0.0066864920222400315 1107 5 0.03413955542757208 1107 6 0.0158586130702858 1107 7 0.09010938250288092 1107 8 0.049920612925845376 1107 9 0.06055392920518443 1107 10-11 0.0067811534471502455 1107 12-13 0.0992836152921086 1107 14-15 0.1403563606276208 1107 16-17 0.1574253151127749 1107 18-19 0.17054616285455637 1107 20-21 0.14678200144628306 1107 22-23 0.08778611215547016 1107 24-25 0.1984351526995951 1107 26-27 0.3329456048814379 1107 28-29 0.1431010071729446 1107 30-31 0.02071250740899444 1107 32-33 0.07458248449049165 1107 34-35 0.03624580406104627 1107 36-37 0.10350043007156273 1107 38-39 0.10400225129198049 1107 40-41 0.04506557724554483 1107 42-43 -0.02702377468833106 1107 44-45 0.07174551716129685 1107 46-47 0.21881284001758416 1107 48-49 0.030187540662829804 1107 50-51 0.12435208464012959 1107 52-53 0.12222277638171164 1107 54-55 0.10716053424746264 1107 56-57 -0.052716705204105097 1107 58-59 0.06744915187861977 1107 60-61 0.12110314773081399 1107 62-63 0.10912388165633757 1107 64-65 0.14578670163422913 1107 66-67 -0.06615605382726386 1107 68-69 0.10287039463752734 1107 70-71 0.23818573943282928 1107 72-73 0.028682119240315274 1107 74-75 0.16484391435790258 1107 76-77 0.047987931851572796 1107 78-79 0.05693987638138509 1107 80-81 0.2109626306686252 1107 82-83 0.18928815026404067 1107 84-85 0.21843824121704358 1107 86-87 0.21942575064978342 1107 88-89 0.3712310009381419 1107 90-91 0.002319887836339518 1107 92-93 0.1669933271523938 1107 94-95 0.3004439239576264 1107 96-97 0.24528379613625262 1107 98-99 0.23111135348377942 1107 100-101 0.37737043510975354 1108 1 -0.17770747963800915 1108 2 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66-67 -0.9409337821219026 2316 68-69 -1.0074030205755022 2316 70-71 -0.8918846577856172 2316 72-73 -0.9060255337743683 2316 74-75 -1.0037732524759875 2316 76-77 -0.8458944318208879 2316 78-79 -0.8582266463072301 2316 80-81 -0.8427437757286498 2316 82-83 -1.0203125029134057 2316 84-85 -1.0008067951172919 2316 86-87 -1.0930335375441942 2316 88-89 -1.1459978955461096 2316 90-91 -1.180945480702757 2316 92-93 -1.1302504321592295 2316 94-95 -1.1480503875679524 2316 96-97 -1.0453070146658554 2316 98-99 -1.1912234875948577 2316 100-101 -1.0674728255549013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 16.0 5 42.0 6 86.0 7 191.0 8 404.0 9 762.0 10 1288.0 11 2155.0 12 3316.0 13 5092.0 14 6393.0 15 8424.0 16 11217.0 17 15616.0 18 14137.0 19 8377.0 20 7094.0 21 7268.0 22 7712.0 23 8584.0 24 10071.0 25 12334.0 26 15008.0 27 18909.0 28 23782.0 29 29859.0 30 37699.0 31 46666.0 32 59380.0 33 77525.0 34 109154.0 35 167287.0 36 285657.0 37 518135.0 38 572589.0 39 110320.0 40 61.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.855564822469795 18.198393505552257 12.438222257833072 23.507819414144873 2 16.470616015606552 19.860345698207933 38.717490890728705 24.951547395456807 3 20.269651841581247 24.334133290475243 28.176560801736844 27.219654066206665 4 12.857472335193846 14.937133901566227 35.80348476222387 36.401909001016065 5 14.032619364774101 38.08599584676053 32.81563121703355 15.065753571431815 6 34.0135239065512 34.541168736476315 16.578725861679146 14.86658149529333 7 30.587010548356545 32.09685020280412 19.234883216245397 18.08125603259394 8 28.345314530254452 34.12448277458915 18.842214128823066 18.687988566333335 9 28.089119402456532 15.164408898864895 19.13282284989953 37.61364884877905 10-11 26.486960493304775 25.981016192578455 25.92751158396342 21.60451173015335 12-13 26.656932788799136 23.0373512592864 27.32009861907418 22.985617332840285 14-15 23.491953666046495 24.403845787149656 25.307316112008525 26.79688443479532 16-17 23.064795820037514 27.93423782355744 25.8708387596769 23.130127596728144 18-19 23.022557701789417 26.158326137351096 28.361726817814077 22.45738934304541 20-21 23.776635397759208 25.384293389581654 28.389177586267955 22.449893626391187 22-23 23.341908744399557 25.376399103701942 28.30375978013412 22.977932371764386 24-25 22.923390822262547 25.664213016709127 28.48180919644288 22.930586964585444 26-27 22.921867984038624 25.882390864840886 28.33505178276075 22.860689368359736 28-29 22.764044005177613 25.90309726095873 28.548429349679168 22.784429384184488 30-31 22.91491115921605 25.774587395214365 28.37007489357993 22.940426551989656 32-33 22.71473268980058 25.857000070144963 28.437517522053103 22.990749718001354 34-35 23.09127688298056 25.813177600707533 28.292476295396508 22.803069220915397 36-37 23.186095633944614 25.771973572351985 28.139520366109867 22.902410427593534 38-39 23.099843390661416 25.907438637128738 28.193154090286793 22.799563881923053 40-41 23.108078536203998 25.85977857098105 28.13865146767323 22.89349142514172 42-43 23.071137786428412 25.88003689384348 27.866066176912923 23.18275914281518 44-45 22.9887338037646 26.085598392385446 27.81777492851364 23.10789287533632 46-47 22.965301603965322 26.307864715487316 27.53937715797905 23.187456522568304 48-49 23.303616612757008 26.294586716451086 27.280626055858537 23.121170614933373 50-51 23.263372581638915 26.45199282806191 27.019445868348896 23.26518872195028 52-53 23.444972817913047 26.46261921036985 26.93570352989144 23.156704441825656 54-55 23.207755713282683 26.3889610886365 26.961146295403836 23.442136902676985 56-57 23.135247069249946 26.58513391633926 26.976997803228574 23.302621211182217 58-59 23.28209123513365 26.262205269402955 27.089756188584907 23.36594730687849 60-61 23.36778605669206 26.373892295116168 27.191772752315636 23.06654889587614 62-63 23.22479431294941 26.482099937438086 27.0986389832463 23.194466766366205 64-65 23.12323730725017 26.330316590060093 27.04887777876651 23.497568323923225 66-67 23.139759141167467 26.564433778349215 26.909069116841867 23.386737963641448 68-69 23.073179280292816 26.388415966817668 27.170235601452454 23.368169151437062 70-71 23.056883904496893 26.257996766561835 27.192297861309022 23.49282146763225 72-73 23.199387989752914 26.54685249440712 26.920165124723194 23.333594391116772 74-75 23.02698425237604 26.69820640710282 26.738907972240305 23.535901368280836 76-77 23.115294543654766 26.617174222380736 26.733835308845954 23.53369592511855 78-79 23.097737287153418 26.461877935765955 26.924487611550585 23.515897165530045 80-81 23.005185934302418 26.78822796318429 26.750000283756524 23.45658581875676 82-83 23.09905757602998 26.7447646391703 26.60758697150391 23.548590813295814 84-85 23.164394955411748 26.532236023096363 26.463680617162094 23.839688404329795 86-87 22.993419190923923 26.554756772814684 26.74345527365942 23.708368762601967 88-89 22.99110624435505 26.505469962308016 26.670076948285132 23.8333468450518 90-91 23.024850542401506 26.602222803180535 26.653393186209186 23.719533468208777 92-93 23.041181403127954 26.478865607471057 26.64643535211617 23.833517637284814 94-95 22.91739545860375 26.538862383121824 26.719061746544533 23.824680411729894 96-97 22.97101118631917 26.431626158057693 26.65103029139094 23.9463323642322 98-99 22.762764630648736 26.334554795779987 26.637680580381566 24.264999993189715 100-101 22.690392825946017 26.54651040314101 26.54673740717805 24.216359363734924 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 363.0 1 259.5 2 387.0 3 647.5 4 503.0 5 437.0 6 996.5 7 1826.5 8 2552.5 9 2703.0 10 2181.5 11 1815.5 12 1842.5 13 1972.5 14 2155.5 15 2323.5 16 2422.0 17 2544.0 18 2754.0 19 2983.5 20 3247.0 21 3676.5 22 4259.5 23 5113.0 24 6221.5 25 7661.5 26 9607.5 27 11707.5 28 13847.0 29 16490.0 30 20221.5 31 24145.0 32 27671.5 33 31207.0 34 35223.0 35 39437.0 36 45104.5 37 50767.0 38 53563.0 39 55322.5 40 56905.0 41 59096.5 42 61628.0 43 65108.0 44 69504.5 45 73614.0 46 76037.0 47 76919.0 48 80862.5 49 83196.0 50 82248.0 51 81160.5 52 80835.0 53 82666.0 54 83141.0 55 79351.5 56 72588.0 57 65475.5 58 61333.5 59 57004.0 60 50139.5 61 43517.5 62 36825.0 63 29228.5 64 22208.0 65 17259.5 66 13221.5 67 10534.0 68 8047.5 69 5759.0 70 4525.5 71 3531.0 72 2803.0 73 2477.5 74 1928.5 75 1481.5 76 1070.5 77 611.5 78 364.5 79 187.0 80 95.0 81 59.0 82 34.0 83 18.5 84 13.0 85 8.0 86 6.0 87 5.5 88 2.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27394673066323805 2 0.02115664177810224 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.8160207534851709E-4 12-13 6.810077825569391E-5 14-15 0.0 16-17 6.583075231383744E-4 18-19 0.0 20-21 1.8160207534851709E-4 22-23 1.1350129709282319E-4 24-25 0.002224625423019334 26-27 0.002315426460693593 28-29 0.002678630611390627 30-31 0.001180413489765361 32-33 0.0016571189375552183 34-35 0.001793320494066606 36-37 2.2700259418564636E-5 38-39 1.5890181592995246E-4 40-41 7.264083013940683E-4 42-43 0.0037455428040631647 44-45 0.004540051883712927 46-47 0.014800569140904142 48-49 0.014641667324974189 50-51 0.0065830752313837445 52-53 0.0071051811980107306 54-55 4.313049289527281E-4 56-57 0.0030645350215062254 58-59 0.00156631789988096 60-61 0.00156631789988096 62-63 0.0 64-65 9.98811414416844E-4 66-67 0.0 68-69 0.0 70-71 4.086046695341635E-4 72-73 0.0018387210129037356 74-75 0.0 76-77 0.1984002673182549 78-79 0.10818943638887905 80-81 0.0011577132303467964 82-83 0.0 84-85 9.080103767425855E-4 86-87 0.12768895922942608 88-89 0.5974935281560398 90-91 0.3183484380859504 92-93 0.29589788152099 94-95 0.0026105298331349333 96-97 0.00279213190848345 98-99 0.0030418347620876614 100-101 6.356072637198098E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2202618.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.380653670764254 #Duplication Level Percentage of deduplicated Percentage of total 1 63.16666293926658 24.243778138098904 2 15.617102277673991 11.98789187720618 3 7.080257292609867 8.152347091426867 4 3.8989643180374913 5.985791966610579 5 2.4263213528292704 4.656189976846021 6 1.6710488798578935 3.8481568994846613 7 1.1427702939281674 3.0702189613546125 8 0.8479177829975796 2.603491101640987 9 0.6256463436262855 2.161144407159038 >10 3.263792340842598 21.822307252656646 >50 0.17953372070599294 4.680606367712393 >100 0.07691221702237157 5.118313749659067 >500 0.002125551185868878 0.5542910762190996 >1k 7.085170619562885E-4 0.4847667498969307 >5k 2.361723539854295E-4 0.6307043840282056 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8002 0.36329495173470844 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5914 0.2684986684027825 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4051 0.18391750180921068 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2700259418564636E-5 0.0 14-15 0.0 0.0 0.0 4.540051883712927E-5 0.0 16-17 0.0 0.0 0.0 1.1350129709282317E-4 0.0 18-19 0.0 0.0 0.0 2.2700259418564634E-4 0.0 20-21 0.0 0.0 0.0 4.086046695341635E-4 0.0 22-23 0.0 0.0 0.0 0.0010215116738354086 0.0 24-25 0.0 0.0 0.0 0.0018841215317408648 0.0 26-27 0.0 0.0 0.0 0.002360826979530722 0.0 28-29 0.0 0.0 0.0 0.0032688373562733074 0.0 30-31 0.0 0.0 0.0 0.0059928684865010635 0.0 32-33 0.0 0.0 0.0 0.011304729190445187 0.0 34-35 0.0 0.0 0.0 0.0182283083131074 0.0 36-37 0.0 0.0 0.0 0.030985854106340728 0.0 38-39 0.0 0.0 0.0 0.052505700035140004 0.0 40-41 0.0 0.0 0.0 0.07670417657532991 0.0 42-43 0.0 0.0 0.0 0.103081878019702 0.0 44-45 0.0 0.0 0.0 0.1302540885437239 0.0 46-47 0.0 0.0 0.0 0.1661885992033117 0.0 48-49 0.0 0.0 0.0 0.20673126252486812 0.0 50-51 0.0 0.0 0.0 0.25101946865048774 0.0 52-53 0.0 0.0 0.0 0.2945812664747133 0.0 54-55 0.0 2.2700259418564636E-5 0.0 0.34266041592323315 0.0 56-57 0.0 4.540051883712927E-5 0.0 0.40109088366661855 0.0 58-59 0.0 4.540051883712927E-5 0.0 0.4664676307920847 0.0 60-61 0.0 4.540051883712927E-5 0.0 0.5426497014007876 0.0 62-63 0.0 4.540051883712927E-5 0.0 0.6185139683776306 0.0 64-65 0.0 4.540051883712927E-5 0.0 0.6944236358733108 0.0 66-67 0.0 4.540051883712927E-5 0.0 0.7827503452709458 0.0 68-69 0.0 4.540051883712927E-5 0.0 0.8917342907394745 0.0 70-71 0.0 4.540051883712927E-5 0.0 1.0121364666955415 0.0 72-73 0.0 4.540051883712927E-5 0.0 1.1328791465428867 0.0 74-75 0.0 4.540051883712927E-5 0.0 1.2542120331351145 0.0 76-77 0.0 4.540051883712927E-5 0.0 1.3862367419134867 0.0 78-79 0.0 4.540051883712927E-5 0.0 1.550450418547383 0.0 80-81 0.0 4.540051883712927E-5 0.0 1.7394981789851895 0.0 82-83 0.0 4.540051883712927E-5 0.0 1.9300895570634582 0.0 84-85 0.0 4.540051883712927E-5 0.0 2.113802756537902 0.0 86-87 0.0 4.540051883712927E-5 0.0 2.3130202331952248 0.0 88-89 0.0 4.540051883712927E-5 0.0 2.543791070444353 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4900 0.0 45.05539 1 GTATCAA 8755 0.0 40.10745 1 ATCAACG 10910 0.0 32.044563 3 TCAACGC 11155 0.0 31.340761 4 GTACATG 13185 0.0 31.142645 1 TATCAAC 11325 0.0 31.047012 2 CAACGCA 11350 0.0 30.80231 5 AACGCAG 11555 0.0 30.378998 6 TACATGG 13560 0.0 29.708986 2 ACATGGG 13480 0.0 28.891157 3 CATGGGA 7755 0.0 25.996735 4 ACGCAGA 13795 0.0 25.30858 7 CGCAGAG 14030 0.0 24.783232 8 CATGGGG 5710 0.0 23.759735 4 AGTACAT 9040 0.0 23.252794 2 GCAGAGT 15275 0.0 22.545874 9 GAGTACA 10345 0.0 22.44472 1 AACGTCG 65 2.9694443E-4 21.894464 22-23 GTGTAGC 1275 0.0 21.644255 1 ATGGGAG 3400 0.0 21.06734 5 >>END_MODULE