FastQCFastQC Report
Thu 26 May 2016
SRR936352_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936352_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2810561
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80360.28592156512525435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53280.18957069424929757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37420.1331406790316951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56800.040.0909651
GTATCAA90350.039.141931
GTACATG164250.031.3886221
ATCAACG114300.030.726613
TACATGG165850.030.5940042
ACATGGG165750.029.898553
TCAACGC118350.029.6730184
CAACGCA118100.029.6556825
TATCAAC120250.029.4424212
AACGCAG122050.028.847436
TCGTGCG700.00854786127.0416979
CATGGGA86850.025.8851014
GTATAGG10150.025.187061
CATGGGG78550.025.125574
ACGCAGA147050.023.7821677
AGTACAT111650.023.3163722
CGCAGAG150050.023.2436058
TACCTGG18650.021.8261222
ATGGGAT34050.021.8227065
ATGGGAG35050.021.740225
Produced by FastQC (version 0.11.5)