FastQCFastQC Report
Thu 26 May 2016
SRR936352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936352_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2810561
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78900.28072687267773233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57770.2055461525296907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39460.14039901642412317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55650.041.0338971
GTATCAA89900.040.1650921
ATCAACG117200.030.7215373
TCAACGC120350.029.917444
GTACATG155900.029.7526191
CAACGCA121600.029.5714155
TACATGG157850.029.3361782
TATCAAC123650.029.2004012
AACGCAG125600.028.7051896
ACATGGG154400.028.6657353
TACGGTC1151.2936152E-424.7508357
ACGCAGA146850.024.454487
CATGGGG70800.024.121154
CGCAGAG151150.023.7901698
AGTACAT109000.023.6794012
CATGGGA83150.023.5051844
GAGTACA129750.022.4393751
TACCTGG17850.022.0616572
ATGGGAG35500.021.5146355
GTATAGG9600.021.3090421
Produced by FastQC (version 0.11.5)