##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936352_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2810561 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49118983718909 34.0 31.0 34.0 31.0 34.0 2 32.61345261675516 34.0 31.0 34.0 31.0 34.0 3 32.70060567979133 34.0 31.0 34.0 31.0 34.0 4 36.188126142787866 37.0 35.0 37.0 35.0 37.0 5 36.1508481758624 37.0 35.0 37.0 35.0 37.0 6 36.19263378378907 37.0 37.0 37.0 35.0 37.0 7 36.13589457763059 37.0 36.0 37.0 35.0 37.0 8 36.11547089709136 37.0 36.0 37.0 35.0 37.0 9 37.87757995645709 39.0 38.0 39.0 35.0 39.0 10-11 37.817037950786336 39.0 38.0 39.0 35.0 39.0 12-13 37.76113078492159 39.0 38.0 39.0 35.0 39.0 14-15 39.10980476851419 40.5 38.5 41.0 36.0 41.0 16-17 39.04479568313941 40.0 38.0 41.0 35.5 41.0 18-19 39.072272403979134 40.0 38.5 41.0 36.0 41.0 20-21 39.030784601366065 40.0 38.5 41.0 35.0 41.0 22-23 38.91420965422917 40.0 38.0 41.0 35.0 41.0 24-25 39.052133008321114 40.0 39.0 41.0 35.0 41.0 26-27 38.96905831255753 40.0 38.5 41.0 35.0 41.0 28-29 38.868961214504864 40.0 38.0 41.0 35.0 41.0 30-31 38.75254032913713 40.0 38.0 41.0 35.0 41.0 32-33 38.59744086678781 40.0 38.0 41.0 34.5 41.0 34-35 38.451733657444194 40.0 38.0 41.0 34.0 41.0 36-37 38.30265452342077 40.0 38.0 41.0 34.0 41.0 38-39 38.161288440279364 40.0 38.0 41.0 33.5 41.0 40-41 38.00690467134497 40.0 37.5 41.0 33.0 41.0 42-43 38.01710156797878 40.0 38.0 41.0 33.0 41.0 44-45 37.868485152964126 40.0 37.0 41.0 33.0 41.0 46-47 37.636861644347874 40.0 37.0 41.0 33.0 41.0 48-49 37.389991179696864 40.0 37.0 41.0 32.5 41.0 50-51 37.17855118604435 40.0 36.5 41.0 32.0 41.0 52-53 36.95895783795477 40.0 36.0 41.0 31.5 41.0 54-55 36.75859036683424 39.0 35.5 41.0 31.0 41.0 56-57 36.50519682725263 39.0 35.0 41.0 31.0 41.0 58-59 36.24066387457878 39.0 35.0 41.0 31.0 41.0 60-61 35.96705622116012 38.0 35.0 40.5 30.0 41.0 62-63 35.67642082843959 38.0 35.0 40.0 30.0 41.0 64-65 35.30768803808208 37.0 35.0 40.0 29.0 41.0 66-67 34.96111114471452 36.5 34.0 39.5 29.0 41.0 68-69 34.618567965612556 36.0 34.0 39.0 29.0 41.0 70-71 34.25570517772074 35.5 34.0 39.0 28.0 40.5 72-73 33.86884984883801 35.0 34.0 38.0 28.0 40.0 74-75 33.458064955715244 35.0 33.5 37.0 27.5 39.0 76-77 32.74126731282473 35.0 32.5 36.0 26.0 39.0 78-79 32.799346464994 35.0 33.0 36.0 26.0 39.0 80-81 32.64728981153584 35.0 33.0 36.0 27.0 37.5 82-83 32.39049517160453 35.0 33.0 35.5 26.0 37.0 84-85 32.12016141973079 35.0 33.0 35.0 26.0 37.0 86-87 31.73186029408364 35.0 32.5 35.0 25.0 36.0 88-89 31.26688959962086 35.0 32.0 35.0 23.5 36.0 90-91 31.06706098889154 35.0 32.0 35.0 23.0 36.0 92-93 30.801735845619433 34.0 32.0 35.0 20.5 35.0 94-95 30.667512820394222 34.0 32.0 35.0 20.0 35.0 96-97 30.44282191348987 34.0 31.0 35.0 19.0 35.0 98-99 30.0305581341234 34.0 31.0 35.0 6.0 35.0 100-101 26.780970418361314 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15853263605445989 1101 2 0.16865182887294594 1101 3 0.19927003646144215 1101 4 0.13854704412634788 1101 5 0.11836310315670318 1101 6 0.14699733816771499 1101 7 0.22574731529968517 1101 8 0.12416714934705197 1101 9 0.25535161416725316 1101 10-11 0.21467639874212097 1101 12-13 0.2677723119720241 1101 14-15 0.41941700422282224 1101 16-17 0.4317986581578239 1101 18-19 0.3575430936623789 1101 20-21 0.32151900808300127 1101 22-23 0.3748539913811655 1101 24-25 0.22805752087014497 1101 26-27 0.3103587198561186 1101 28-29 0.3315653197539632 1101 30-31 0.31840468349907525 1101 32-33 0.3644024264527346 1101 34-35 0.34907209198894407 1101 36-37 0.29729809298360976 1101 38-39 0.30626745908737973 1101 40-41 0.34281413497651414 1101 42-43 0.3822538176980359 1101 44-45 0.4687398855378646 1101 46-47 0.5341187642040524 1101 48-49 0.5207201033433293 1101 50-51 0.40964630742683283 1101 52-53 0.5134088712972229 1101 54-55 0.4758321560587575 1101 56-57 0.3104337523384473 1101 58-59 0.2596128081473523 1101 60-61 0.37948590720595377 1101 62-63 0.3938687205074487 1101 64-65 0.47260521788575716 1101 66-67 0.3129919725809529 1101 68-69 0.3735383964639283 1101 70-71 0.3862441750320116 1101 72-73 0.44450917742033624 1101 74-75 0.34178567518557657 1101 76-77 0.33682182920064463 1101 78-79 0.2522121426327715 1101 80-81 0.3573688315384729 1101 82-83 0.35812153990230655 1101 84-85 0.4402779642115817 1101 86-87 0.47793510809203 1101 88-89 0.3985250503534239 1101 90-91 0.5452408784928622 1101 92-93 0.6240453571110685 1101 94-95 0.48192921793578947 1101 96-97 0.3469915603818272 1101 98-99 0.5493217963668258 1101 100-101 0.757199309962072 1102 1 0.04293583242828447 1102 2 0.15993999777007417 1102 3 0.11221895664772319 1102 4 0.06862076409447582 1102 5 0.07114420875607408 1102 6 0.08268306833377892 1102 7 0.10732252384902807 1102 8 0.1584800454744837 1102 9 0.08285547654067926 1102 10-11 0.10336793730879634 1102 12-13 0.16463509477294735 1102 14-15 0.29528534326453126 1102 16-17 0.26807987213116746 1102 18-19 0.3245329947811726 1102 20-21 0.2707607238030718 1102 22-23 0.2629497724256922 1102 24-25 0.23718710929365017 1102 26-27 0.28052645061784887 1102 28-29 0.2161806451521926 1102 30-31 0.15804634625532543 1102 32-33 0.08661476452686401 1102 34-35 0.21499234960938196 1102 36-37 0.03899961410267849 1102 38-39 0.060781829887879724 1102 40-41 -0.02309842441319887 1102 42-43 0.06381222699722144 1102 44-45 0.04613156758245651 1102 46-47 0.09103751485305622 1102 48-49 0.07638866132271716 1102 50-51 0.008713555577934073 1102 52-53 -0.03866957401599791 1102 54-55 -0.132980453437888 1102 56-57 -0.04043270063674953 1102 58-59 -0.02799317724414152 1102 60-61 -0.11121428793563837 1102 62-63 0.025097979121646574 1102 64-65 -0.004590484311776777 1102 66-67 -0.03583196871131378 1102 68-69 0.00813392030200788 1102 70-71 -0.08611857791688493 1102 72-73 -0.1993664780198543 1102 74-75 -0.014129705178014262 1102 76-77 -0.18919921707787068 1102 78-79 -0.23789357802512257 1102 80-81 -0.1386073887978867 1102 82-83 -0.07149251015569291 1102 84-85 -0.05915561707892181 1102 86-87 -0.18684851431556737 1102 88-89 -0.20050019967227684 1102 90-91 -0.05310880901884474 1102 92-93 -0.23942463920575463 1102 94-95 -0.34137218028530114 1102 96-97 -0.4693142845084033 1102 98-99 -0.2820992679426553 1102 100-101 0.3250006217063728 1103 1 0.11476386942676697 1103 2 0.1639307191244157 1103 3 0.12400575888578658 1103 4 0.06010220086630369 1103 5 0.11301932501949352 1103 6 0.15003768463480327 1103 7 0.20362442351089527 1103 8 0.18943002812567045 1103 9 0.14687473438095822 1103 10-11 0.26038820309523203 1103 12-13 0.2501977173855039 1103 14-15 0.3672004541452054 1103 16-17 0.4654695191785052 1103 18-19 0.2417307626553864 1103 20-21 0.283355020859581 1103 22-23 0.3299071965955491 1103 24-25 0.27459094872413203 1103 26-27 0.27918447388240963 1103 28-29 0.40826760821306607 1103 30-31 0.3075953266333613 1103 32-33 0.30387729360096216 1103 34-35 0.2697384863655117 1103 36-37 0.2827034962785291 1103 38-39 0.2525764852666086 1103 40-41 0.32362532182184367 1103 42-43 0.3148635451770545 1103 44-45 0.34363540015257144 1103 46-47 0.4073320726034879 1103 48-49 0.38859396240705735 1103 50-51 0.4310608035815022 1103 52-53 0.3570224291714297 1103 54-55 0.42180625183153353 1103 56-57 0.373040020171409 1103 58-59 0.39248511563282307 1103 60-61 0.37592780212778365 1103 62-63 0.38987009621754254 1103 64-65 0.34460629210755656 1103 66-67 0.3604133988921916 1103 68-69 0.40749292130072234 1103 70-71 0.25138044909844837 1103 72-73 0.2737365152108069 1103 74-75 0.31304447029169324 1103 76-77 0.3153075159760377 1103 78-79 0.2469479804918251 1103 80-81 0.2565566465224478 1103 82-83 0.33199145109906425 1103 84-85 0.30207673231954857 1103 86-87 0.25655527630076236 1103 88-89 0.34444567269588333 1103 90-91 0.40343893253531604 1103 92-93 0.5446349364805769 1103 94-95 0.5552175249432203 1103 96-97 0.4972837290197134 1103 98-99 0.3773941929407201 1103 100-101 0.8227892254502862 1104 1 -0.05919559206396485 1104 2 0.05054182745193714 1104 3 -0.014856525294888456 1104 4 0.020359745147892738 1104 5 0.015336953759515382 1104 6 0.05167745538679469 1104 7 0.09415622004493684 1104 8 0.04769154480602111 1104 9 0.09710235719835225 1104 10-11 0.04223612651478703 1104 12-13 0.14995117685406711 1104 14-15 0.14638737143786074 1104 16-17 0.1357804152679023 1104 18-19 0.1689397189243138 1104 20-21 0.13177041339090323 1104 22-23 0.14928181037227972 1104 24-25 0.17447448755073225 1104 26-27 0.24619899844717708 1104 28-29 0.20704736747953945 1104 30-31 0.03542490597277492 1104 32-33 0.03375995880816873 1104 34-35 0.14542509660664393 1104 36-37 0.08999678893717089 1104 38-39 0.24975994598135998 1104 40-41 0.22803867355256813 1104 42-43 0.29114815110405345 1104 44-45 0.3179571728034105 1104 46-47 0.3638502368208094 1104 48-49 0.2884594346561542 1104 50-51 0.2977233590980859 1104 52-53 0.3812431253433033 1104 54-55 0.3133654344064212 1104 56-57 0.3027047462467607 1104 58-59 0.33885821406531846 1104 60-61 0.3217730353134911 1104 62-63 0.2049793114889198 1104 64-65 0.10524116288254959 1104 66-67 0.08546144795877098 1104 68-69 0.22442107959541602 1104 70-71 0.16165395620495815 1104 72-73 0.2775604468577981 1104 74-75 0.22254777912514356 1104 76-77 0.23727082139208733 1104 78-79 0.13403492232290404 1104 80-81 0.12110934696991649 1104 82-83 0.10640212737040855 1104 84-85 0.1628263780540351 1104 86-87 0.1583744280934205 1104 88-89 0.23793629285002638 1104 90-91 0.4567463367166127 1104 92-93 0.4264074138724645 1104 94-95 0.3104613289903888 1104 96-97 0.1087952222536579 1104 98-99 -0.0282981118856398 1104 100-101 -0.051063265842426375 1105 1 -0.12135263542759844 1105 2 -0.06576183750988918 1105 3 -0.020541166680551726 1105 4 0.017561747791873472 1105 5 -0.0720777623591431 1105 6 -0.04132153866616761 1105 7 0.0313105076532878 1105 8 0.04265009285059307 1105 9 0.031065672892282237 1105 10-11 0.049740953904290564 1105 12-13 0.050844966576441664 1105 14-15 0.040598512397636455 1105 16-17 0.08610867775455944 1105 18-19 0.03615632899843746 1105 20-21 0.025175409209097666 1105 22-23 -0.04367694579926962 1105 24-25 0.07028674160439863 1105 26-27 0.07386893990182841 1105 28-29 -0.077557634505375 1105 30-31 -0.04812473809155904 1105 32-33 0.02691936479837409 1105 34-35 0.025846900604989287 1105 36-37 -0.07317838074412464 1105 38-39 0.05132243995916497 1105 40-41 0.11318842861796696 1105 42-43 0.07005771993433996 1105 44-45 0.017187180087852028 1105 46-47 0.06001815674588329 1105 48-49 0.07834315334556408 1105 50-51 0.0315786029342533 1105 52-53 0.0625645003688362 1105 54-55 0.0024777728671381283 1105 56-57 0.12662416256623032 1105 58-59 0.030688999127960415 1105 60-61 0.11858152381062581 1105 62-63 0.15868886644408065 1105 64-65 0.15274545068361078 1105 66-67 0.01350401377892041 1105 68-69 -0.019974716239730128 1105 70-71 -0.004865505594104036 1105 72-73 -0.0028249734623315703 1105 74-75 0.09343281980625306 1105 76-77 0.02178000788541823 1105 78-79 -0.05670897743698333 1105 80-81 0.04577994749332248 1105 82-83 0.06470180708612361 1105 84-85 0.1259521368502945 1105 86-87 0.14945171643021737 1105 88-89 0.2877870966441023 1105 90-91 0.31043731441234 1105 92-93 0.37393914360031744 1105 94-95 0.18772561555810796 1105 96-97 0.05711382610709137 1105 98-99 0.19223238064298442 1105 100-101 0.3077002286865316 1106 1 -0.038673724984462865 1106 2 -0.016197667713278463 1106 3 -0.03616153004023204 1106 4 -0.007034517160072085 1106 5 -0.00354101051567568 1106 6 0.00991463766510492 1106 7 0.02203114739309342 1106 8 0.0404027979071131 1106 9 -0.012412315537794427 1106 10-11 0.036564745720305325 1106 12-13 0.024404600904269103 1106 14-15 0.039449427510511725 1106 16-17 -0.014568061929672638 1106 18-19 0.06996705523291524 1106 20-21 0.1392720639278835 1106 22-23 0.09816648355222668 1106 24-25 0.12321625907676292 1106 26-27 -0.02968454021260669 1106 28-29 0.04193525831060896 1106 30-31 0.13551691511665354 1106 32-33 0.19245308429305652 1106 34-35 0.26264723181356686 1106 36-37 0.14646467487877146 1106 38-39 0.23421447267720197 1106 40-41 0.048492353514177466 1106 42-43 0.12817781288161 1106 44-45 0.12655337070435024 1106 46-47 0.21092998738193103 1106 48-49 0.25971408830113063 1106 50-51 0.29004601730676427 1106 52-53 0.241828531042934 1106 54-55 0.2324766166209855 1106 56-57 0.299492585483776 1106 58-59 0.27468088302592975 1106 60-61 0.32020668372692995 1106 62-63 0.2656605085747046 1106 64-65 0.2360461231712918 1106 66-67 0.22561296946485498 1106 68-69 0.36810960767330414 1106 70-71 0.33273293384656455 1106 72-73 0.2821748586954911 1106 74-75 0.39985990729678633 1106 76-77 0.27018159082369664 1106 78-79 0.18138478293660398 1106 80-81 0.2825731174353905 1106 82-83 0.26907308891552617 1106 84-85 0.4857511738091418 1106 86-87 0.36516890608069374 1106 88-89 0.4191882058944465 1106 90-91 0.6686677356216251 1106 92-93 0.5849134861251848 1106 94-95 0.5680231065039543 1106 96-97 0.6317036384595269 1106 98-99 0.4123661322141743 1106 100-101 0.758761550760763 1107 1 -0.05373475758089086 1107 2 0.09785269195663915 1107 3 0.08151308020772063 1107 4 0.053423368939867544 1107 5 0.011174391009184603 1107 6 0.04666622566419676 1107 7 0.014839606485267609 1107 8 0.05034870734093744 1107 9 0.0491689876321999 1107 10-11 0.03256274653941915 1107 12-13 0.0036934367265715196 1107 14-15 0.08374012228004801 1107 16-17 0.12827260756375125 1107 18-19 0.11824930131171385 1107 20-21 0.07647470589279237 1107 22-23 0.03789874871580423 1107 24-25 0.08057330974940413 1107 26-27 0.1684732755847662 1107 28-29 0.14776871521259238 1107 30-31 0.05563248869861326 1107 32-33 0.032225608588618115 1107 34-35 0.03868379224421403 1107 36-37 0.06164993653835893 1107 38-39 0.10934994674589404 1107 40-41 0.10377150052207895 1107 42-43 -0.012402478934411931 1107 44-45 0.041263989425765146 1107 46-47 0.08486728405473798 1107 48-49 -0.007555237397887993 1107 50-51 0.0039578304598180125 1107 52-53 -0.03182305116726525 1107 54-55 0.06900116850015792 1107 56-57 -0.022237857570992503 1107 58-59 -0.10071153129300114 1107 60-61 0.07648964676189252 1107 62-63 0.06859608189044053 1107 64-65 0.17345393037818724 1107 66-67 0.038122353201409 1107 68-69 0.18430229181046087 1107 70-71 0.27079075345159964 1107 72-73 0.13188818457651763 1107 74-75 0.07820801675828903 1107 76-77 0.03699949107702594 1107 78-79 0.026713098402268542 1107 80-81 0.23085313463491275 1107 82-83 0.21817113576718583 1107 84-85 0.1794258465895453 1107 86-87 0.17550211424594764 1107 88-89 0.26106686632561704 1107 90-91 -0.007941615827132154 1107 92-93 0.23549293282448147 1107 94-95 0.273809184242932 1107 96-97 0.24216581205640964 1107 98-99 0.19917150740420908 1107 100-101 0.19585963661781136 1108 1 -0.1208810681599175 1108 2 -0.06154404882607878 1108 3 -0.017915184248749938 1108 4 -0.026155311847517737 1108 5 -0.0810105124569418 1108 6 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-0.771256020529087 2316 78-79 -0.998060680830335 2316 80-81 -0.8737465668952211 2316 82-83 -1.029690758948071 2316 84-85 -0.8187112801420149 2316 86-87 -0.9900235014456733 2316 88-89 -0.8763843035358398 2316 90-91 -1.1158743051877629 2316 92-93 -1.130122680782982 2316 94-95 -0.9680643104513713 2316 96-97 -0.8912405226823381 2316 98-99 -1.366604987926486 2316 100-101 -1.558658927472404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 25.0 5 74.0 6 124.0 7 292.0 8 479.0 9 926.0 10 1504.0 11 2615.0 12 3886.0 13 5810.0 14 7117.0 15 9512.0 16 12291.0 17 17003.0 18 15524.0 19 9615.0 20 8544.0 21 8694.0 22 9299.0 23 10492.0 24 12716.0 25 15481.0 26 19181.0 27 24396.0 28 31039.0 29 38543.0 30 48649.0 31 60810.0 32 76383.0 33 99588.0 34 140701.0 35 214705.0 36 374148.0 37 666275.0 38 731819.0 39 132164.0 40 127.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.383490471292575 18.20038951804723 12.352160276793994 24.0639597338662 2 16.604969334017568 19.850823180735343 38.19037607021325 25.353831415033838 3 19.93448994702481 24.21210569704767 28.16587151106131 27.687532844866205 4 13.020354299372972 14.917448865190972 35.42744669124776 36.634750144188295 5 14.070785156415392 37.95615893054803 32.97718853993918 14.995867373097399 6 34.55068223034476 34.15396427972921 16.525739878977898 14.769613610948134 7 31.00441513277954 31.638452252059285 19.509770469312 17.847362145849175 8 28.36053015750236 33.89889064852177 18.943904793384668 18.796674400591197 9 27.82939064478586 15.196894854799451 19.115080583556093 37.858633916858594 10-11 26.513515230018026 25.888086377353343 26.11261526429955 21.485783128329082 12-13 26.492860322191902 22.821707125374612 27.40433315626311 23.281099396170372 14-15 23.267881394497397 24.397958272387612 25.025021694956983 27.309138638158004 16-17 23.299028641562312 27.853193068897408 25.572890843193065 23.27488744634722 18-19 23.143724686993096 26.17767413694277 27.814642699446836 22.863958476617302 20-21 23.821608346386792 25.594299351641087 27.637808513079698 22.946283788892416 22-23 23.492030679300687 25.41949387657511 27.702366735164297 23.386108708959906 24-25 23.328547690972037 25.747449732964718 27.888286384224813 23.035716191838436 26-27 23.16512010981713 26.06128716677747 27.680257236236876 23.09333548716852 28-29 22.85900424230111 25.884405745163768 28.1761307495491 23.080459262986018 30-31 23.03277587570305 25.68688618061912 27.983358995775703 23.29697894790213 32-33 22.94310159194853 25.749879559568733 27.911082583352965 23.395936265129773 34-35 23.41098877482709 25.843924962466826 27.630093219619834 23.114993043086255 36-37 23.584965911591624 25.587627659190275 27.57685791743601 23.250548511782085 38-39 23.132144826122513 25.698629435131444 27.810862368360144 23.358363370385902 40-41 23.07475815171659 25.813997508666475 27.9875632573492 23.123681082267733 42-43 23.536738409600453 25.61560404362147 27.49040990218296 23.35724764459512 44-45 23.111151041379422 25.972040114501034 27.41098861216422 23.505820231955322 46-47 23.299502736125614 26.122046361476038 27.220802383336572 23.357648519061783 48-49 23.60051933013743 26.18606744067995 26.948230856017553 23.26518237316507 50-51 23.582194865021474 26.15799257861405 26.74387180576087 23.515940750603608 52-53 23.660425495575314 26.179613360518367 26.732494297903838 23.427466846002485 54-55 23.52935734786288 26.055910438834267 26.736418176025072 23.678314037277776 56-57 23.41724094742805 26.516827066770276 26.572155851401476 23.4937761344002 58-59 23.455286078757073 25.959245743282803 26.91669911904469 23.66876905891543 60-61 23.46036683808739 26.150018243880208 27.041059671453603 23.348555246578805 62-63 23.322390085111124 26.261874408703456 26.966947168198807 23.448788337986613 64-65 23.379880834033674 26.119893941863005 26.764397797713883 23.735827426389434 66-67 23.30863838215929 26.434242131730993 26.738024899655265 23.519094586454447 68-69 23.237709482199463 26.180467173635442 26.968352581566457 23.61347076259864 70-71 23.3323845290343 26.17290890945027 26.939038730784485 23.555667830730943 72-73 23.25006520148058 26.413537855463705 26.559525126853796 23.77687181620192 74-75 23.26537299848678 26.510508044479376 26.441269198569255 23.782849758464593 76-77 23.179141665511008 26.61364181648941 26.544872670128772 23.66234384787081 78-79 23.319135052964846 26.460197384472313 26.579675931581836 23.640991630981006 80-81 23.11921049724278 26.807982911399048 26.472779052053735 23.600027539304442 82-83 23.489029414412283 26.72192491107647 26.301759684276554 23.487285990234692 84-85 23.271868946344362 26.50739972535561 26.234054018377584 23.98667730992244 86-87 23.22849168708063 26.153407432172592 26.713628428419085 23.904472452327692 88-89 23.361881839249126 26.355484373182534 26.46396392365989 23.818669863908447 90-91 23.149032974982926 26.641901032757985 26.561008527708225 23.648057464550863 92-93 23.20917334878687 26.39564455771329 26.386295358336614 24.00888673516323 94-95 23.030561260842145 26.358240207934347 26.585744008411304 24.0254545228122 96-97 23.198929293597786 26.312826879100054 26.5131128799661 23.97513094733606 98-99 23.1230553800714 26.386005087745733 26.35641937840834 24.134520153774528 100-101 22.963846145087928 26.434579924740547 26.426343085182086 24.17523084498944 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 311.0 1 231.0 2 360.0 3 609.5 4 476.5 5 412.0 6 910.0 7 1656.0 8 2408.0 9 2630.5 10 2130.0 11 1806.5 12 1869.5 13 2100.5 14 2331.5 15 2417.0 16 2489.0 17 2638.0 18 2800.5 19 3013.5 20 3407.5 21 3970.0 22 4731.0 23 5769.5 24 6857.0 25 8315.0 26 10485.5 27 12958.5 28 15528.0 29 18772.5 30 23000.5 31 28392.5 32 34228.0 33 38782.5 34 42690.0 35 48137.5 36 56664.0 37 63873.5 38 68027.5 39 71521.5 40 73253.0 41 76830.0 42 81161.5 43 85665.5 44 92339.5 45 99439.5 46 100719.0 47 98564.0 48 101778.5 49 102931.5 50 100976.0 51 99987.5 52 101935.0 53 106064.0 54 107952.5 55 103217.0 56 93890.0 57 86351.5 58 82298.5 59 76398.0 60 66109.0 61 56507.0 62 48554.0 63 38666.0 64 29847.5 65 23777.5 66 17466.5 67 14090.0 68 10911.5 69 7496.0 70 5699.5 71 4303.5 72 3690.0 73 3286.5 74 2725.5 75 2353.5 76 1622.5 77 878.5 78 512.0 79 278.0 80 177.0 81 121.5 82 69.5 83 39.0 84 24.0 85 16.0 86 12.5 87 8.0 88 5.0 89 2.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2707644488057722 2 0.021597111750999178 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.380108099415028E-4 12-13 0.0 14-15 0.0 16-17 4.4475106571250365E-4 18-19 0.0 20-21 1.2453029839950102E-4 22-23 5.337012788550044E-5 24-25 0.0025261860532470207 26-27 0.0025083960106185204 28-29 0.002063644944906017 30-31 0.0011919328561095098 32-33 0.0015299436660510125 34-35 0.001690054049707514 36-37 5.337012788550044E-5 38-39 1.6011038365650133E-4 40-41 6.404415346260053E-4 42-43 0.003913809378270032 44-45 0.003771489037242031 46-47 0.013627172653431113 48-49 0.014320984315942616 50-51 0.00603082445106155 52-53 0.006795796284087057 54-55 4.4475106571250365E-4 56-57 0.003166627587873026 58-59 0.0011563527708525097 60-61 0.0010496125150815086 62-63 0.0 64-65 9.606623019390078E-4 66-67 0.0 68-69 0.0 70-71 2.1348051154200176E-4 72-73 0.0014943635807940123 74-75 0.0 76-77 0.1965443909596696 78-79 0.10474777099660885 80-81 0.0011563527708525097 82-83 0.0 84-85 6.226514919975051E-4 86-87 0.12291140452030751 88-89 0.5865732855469068 90-91 0.30935105126698903 92-93 0.29111625757277637 94-95 0.0020814349875345173 96-97 0.0028464068205600233 98-99 0.0029353570337025243 100-101 6.04861449369005E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2810561.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.58162556744576 #Duplication Level Percentage of deduplicated Percentage of total 1 62.23850449220794 20.2783164924291 2 15.380769623811116 10.022609536443147 3 7.1603201875719735 6.998846138834793 4 3.962278907744473 5.163899510636735 5 2.5443486233429944 4.144950707940376 6 1.7143501719847694 3.35137892370564 7 1.2273571209106227 2.7992503105733717 8 0.9257689012039333 2.4130444560809794 9 0.6831780765984217 2.00331470588558 >10 3.686368921403264 21.542100210458987 >50 0.2833962578979788 6.397753192056448 >100 0.18145714605737573 11.15530303485111 >500 0.009499417670349673 2.0706694328313775 >1k 0.002183774177090532 1.1723535862811199 >5k 2.183774177090532E-4 0.4862097609912018 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7890 0.28072687267773233 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5777 0.2055461525296907 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3946 0.14039901642412317 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.116017051400059E-5 0.0 14-15 0.0 0.0 0.0 2.1348051154200176E-4 0.0 16-17 0.0 0.0 0.0 8.005519182825066E-4 0.0 18-19 0.0 0.0 0.0 0.0012986731118805107 0.0 20-21 0.0 0.0 0.0 0.0022771254564480188 0.0 22-23 0.0 0.0 0.0 0.00482110155232354 0.0 24-25 0.0 0.0 0.0 0.007400657733456061 0.0 26-27 0.0 0.0 0.0 0.008165629566481567 0.0 28-29 0.0 0.0 0.0 0.009126291868420575 0.0 30-31 1.7790042628500147E-5 0.0 0.0 0.012666510351492105 0.0 32-33 3.558008525700029E-5 0.0 0.0 0.01793236296952815 0.0 34-35 3.558008525700029E-5 0.0 0.0 0.025368600788241207 0.0 36-37 3.558008525700029E-5 0.0 0.0 0.037679310287163315 0.0 38-39 3.558008525700029E-5 0.0 0.0 0.05790658875576797 0.0 40-41 3.558008525700029E-5 0.0 0.0 0.08265253805201167 0.0 42-43 5.337012788550044E-5 0.0 0.0 0.10467661082609486 0.0 44-45 7.116017051400059E-5 0.0 0.0 0.13123714447044557 0.0 46-47 7.116017051400059E-5 0.0 0.0 0.16452231422836935 0.0 48-49 7.116017051400059E-5 0.0 0.0 0.20471002052615117 0.0 50-51 7.116017051400059E-5 0.0 0.0 0.24829562496597657 0.0 52-53 7.116017051400059E-5 0.0 0.0 0.2907248766349494 0.0 54-55 7.116017051400059E-5 0.0 0.0 0.3370857277248208 0.0 56-57 7.116017051400059E-5 0.0 0.0 0.39066933612186316 0.0 58-59 7.116017051400059E-5 0.0 0.0 0.45627901333577175 0.0 60-61 7.116017051400059E-5 0.0 0.0 0.5306947616507879 0.0 62-63 7.116017051400059E-5 0.0 0.0 0.606676033717112 0.0 64-65 7.116017051400059E-5 0.0 0.0 0.6823370850161231 0.0 66-67 7.116017051400059E-5 0.0 0.0 0.7664484065636719 0.0 68-69 7.116017051400059E-5 0.0 0.0 0.8706980563666826 0.0 70-71 7.116017051400059E-5 0.0 0.0 0.9930401795228783 0.0 72-73 7.116017051400059E-5 0.0 0.0 1.1158448437874147 0.0 74-75 7.116017051400059E-5 0.0 0.0 1.2347179086310527 0.0 76-77 7.116017051400059E-5 0.0 0.0 1.3668089751476664 0.0 78-79 7.116017051400059E-5 0.0 0.0 1.519856711880653 0.0 80-81 7.116017051400059E-5 0.0 0.0 1.6953376923681784 0.0 82-83 7.116017051400059E-5 0.0 0.0 1.8835385533350815 0.0 84-85 7.116017051400059E-5 0.0 0.0 2.063182403797676 0.0 86-87 7.116017051400059E-5 0.0 0.0 2.259246463606376 0.0 88-89 7.116017051400059E-5 0.0 0.0 2.486745528739636 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5565 0.0 41.033897 1 GTATCAA 8990 0.0 40.165092 1 ATCAACG 11720 0.0 30.721537 3 TCAACGC 12035 0.0 29.91744 4 GTACATG 15590 0.0 29.752619 1 CAACGCA 12160 0.0 29.571415 5 TACATGG 15785 0.0 29.336178 2 TATCAAC 12365 0.0 29.200401 2 AACGCAG 12560 0.0 28.705189 6 ACATGGG 15440 0.0 28.665735 3 TACGGTC 115 1.2936152E-4 24.750835 7 ACGCAGA 14685 0.0 24.45448 7 CATGGGG 7080 0.0 24.12115 4 CGCAGAG 15115 0.0 23.790169 8 AGTACAT 10900 0.0 23.679401 2 CATGGGA 8315 0.0 23.505184 4 GAGTACA 12975 0.0 22.439375 1 TACCTGG 1785 0.0 22.061657 2 ATGGGAG 3550 0.0 21.514635 5 GTATAGG 960 0.0 21.309042 1 >>END_MODULE