FastQCFastQC Report
Thu 26 May 2016
SRR936351_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936351_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066755
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47110.2279418702265145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34690.167847664575627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23810.11520475334521993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69200.031.8896261
GGTATCA47350.030.0034281
GTATAGG5700.029.908681
GTACATG76650.029.0990221
TACATGG80800.027.478592
TCAACGC80550.026.97424
ATCAACG80900.026.85883
ACATGGG79300.026.5649643
CAACGCA82500.026.1644925
TATCAAC83250.026.1581212
AACGCAG84800.025.6210696
GTACCTG18000.024.2038761
CATGGGG45550.023.2788264
ATACCGT1652.5061709E-622.95026
TATAACG1903.397945E-722.4244542
AGTACAT54950.022.3133622
TACCTGG18300.022.2474542
ACGCAGA97100.022.229857
GAGTACA62200.022.0027541
CGCAGAG100000.021.5388898
Produced by FastQC (version 0.11.5)