##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936351_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2066755 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.464869324133726 33.0 31.0 34.0 30.0 34.0 2 31.558393229966782 33.0 31.0 34.0 29.0 34.0 3 31.625315530868438 34.0 31.0 34.0 28.0 34.0 4 35.10940967845729 37.0 35.0 37.0 32.0 37.0 5 35.08172279733205 37.0 35.0 37.0 32.0 37.0 6 35.171288807817085 37.0 35.0 37.0 32.0 37.0 7 35.11140169008905 37.0 35.0 37.0 32.0 37.0 8 35.10923113770137 37.0 35.0 37.0 32.0 37.0 9 36.73845037268568 39.0 37.0 39.0 33.0 39.0 10-11 36.64924192756277 39.0 37.0 39.0 32.5 39.0 12-13 36.54247310397217 39.0 37.0 39.0 32.0 39.0 14-15 37.73804393844456 40.0 38.0 41.0 32.0 41.0 16-17 37.67395990332672 40.0 38.0 41.0 32.0 41.0 18-19 37.61620995231655 40.0 38.0 41.0 32.0 41.0 20-21 37.530637400175635 40.0 38.0 41.0 32.0 41.0 22-23 37.35992872885272 40.0 37.5 41.0 32.0 41.0 24-25 37.51997672680119 40.0 38.0 41.0 32.0 41.0 26-27 37.46560453464489 40.0 38.0 41.0 32.0 41.0 28-29 37.31978923481496 40.0 37.5 41.0 31.5 41.0 30-31 37.160873204613026 40.0 37.0 41.0 31.0 41.0 32-33 36.96714463010855 40.0 37.0 41.0 30.5 41.0 34-35 36.69551156281223 40.0 36.0 41.0 30.0 41.0 36-37 36.49807935628558 40.0 36.0 41.0 30.0 41.0 38-39 36.32538689878578 40.0 36.0 41.0 30.0 41.0 40-41 36.1354788061478 40.0 35.5 41.0 29.0 41.0 42-43 35.943827400925606 39.5 35.0 41.0 28.5 41.0 44-45 35.755149739567585 39.0 35.0 41.0 27.5 41.0 46-47 35.47006902124345 39.0 35.0 41.0 26.5 41.0 48-49 35.283789079982874 39.0 35.0 41.0 26.0 41.0 50-51 34.97465398656348 38.5 34.5 40.5 25.5 41.0 52-53 34.99538842291418 39.0 35.0 40.0 26.0 41.0 54-55 35.07213578774456 39.0 35.0 41.0 26.0 41.0 56-57 34.90555629477127 39.0 35.0 41.0 25.0 41.0 58-59 34.66551889314408 38.5 34.5 40.5 24.5 41.0 60-61 34.366847546032304 38.0 34.0 40.0 23.5 41.0 62-63 34.054421302960435 37.5 34.0 40.0 23.0 41.0 64-65 33.717245633855974 37.0 34.0 40.0 22.0 41.0 66-67 33.34768126846191 36.5 33.0 39.5 21.5 41.0 68-69 32.954417916008424 36.0 33.0 39.0 20.5 41.0 70-71 32.54139532745778 35.0 33.0 39.0 19.0 40.5 72-73 32.12779768284098 35.0 32.0 38.0 18.0 40.0 74-75 31.70548323337793 35.0 32.0 37.0 14.5 39.5 76-77 31.29953260062272 35.0 31.5 37.0 10.0 39.0 78-79 30.897642923326664 35.0 31.0 36.0 7.5 39.0 80-81 30.495075371778462 35.0 31.0 36.0 6.0 37.5 82-83 30.1403281956497 34.5 31.0 35.0 2.0 37.0 84-85 29.814104477792483 34.0 30.0 35.0 2.0 36.5 86-87 29.48204649317408 34.0 30.0 35.0 2.0 36.0 88-89 29.147479986742503 34.0 30.0 35.0 2.0 36.0 90-91 28.780298100161847 34.0 29.0 35.0 2.0 35.5 92-93 28.443538300379096 34.0 29.0 35.0 2.0 35.0 94-95 28.044428342982116 34.0 29.0 35.0 2.0 35.0 96-97 27.596239515569096 34.0 28.0 35.0 2.0 35.0 98-99 27.063389709955942 34.0 27.0 35.0 2.0 35.0 100-101 23.76166115480548 29.5 13.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15848412997409866 1101 2 0.19121157956951151 1101 3 0.2717712958484704 1101 4 0.026940815276660146 1101 5 0.35780262582640887 1101 6 0.2052544651201771 1101 7 0.2855569596524532 1101 8 0.30211187516194826 1101 9 0.4014139261143512 1101 10-11 0.4981315852630388 1101 12-13 0.3911005721268239 1101 14-15 0.55295363807803 1101 16-17 0.5278418443714443 1101 18-19 0.4864749623668061 1101 20-21 0.5337793534722337 1101 22-23 0.4419797165583361 1101 24-25 0.44548663899958996 1101 26-27 0.4125285973662187 1101 28-29 0.3822683419077819 1101 30-31 0.4507132432692984 1101 32-33 0.5088985582620325 1101 34-35 0.40838141921759785 1101 36-37 0.33234069946087175 1101 38-39 0.4341180307076016 1101 40-41 0.3748040802330266 1101 42-43 0.2824433053353843 1101 44-45 0.3873745982617578 1101 46-47 0.39253971290412437 1101 48-49 0.3430290913411227 1101 50-51 0.46553426073251813 1101 52-53 0.49200023661531134 1101 54-55 0.4283619264176224 1101 56-57 0.48731581480355857 1101 58-59 0.6029253631759843 1101 60-61 0.4762255406385094 1101 62-63 0.39322681461381137 1101 64-65 0.34126565995592983 1101 66-67 0.48238787124956417 1101 68-69 0.5442058080081509 1101 70-71 0.6834834875423113 1101 72-73 0.6102610313361438 1101 74-75 0.41721174649666537 1101 76-77 0.3580343346623316 1101 78-79 0.4681887862850225 1101 80-81 0.4692895690430454 1101 82-83 0.5792500631013802 1101 84-85 0.5460041374231501 1101 86-87 0.3915337753106556 1101 88-89 0.45401025854662436 1101 90-91 0.5668017334624693 1101 92-93 0.4441028840295651 1101 94-95 0.552514248553976 1101 96-97 0.7022498488623761 1101 98-99 0.968708522075211 1101 100-101 1.0803109497219232 1102 1 0.30822329353905786 1102 2 0.31236741712800153 1102 3 0.24059181693518994 1102 4 0.12337458529121648 1102 5 0.253375244839134 1102 6 0.2332956030313369 1102 7 0.20611419224626104 1102 8 0.19946125921673286 1102 9 0.19059556401085587 1102 10-11 0.39551699307641286 1102 12-13 0.3848928847788571 1102 14-15 0.5039369035157435 1102 16-17 0.5173766372165218 1102 18-19 0.5037736933535868 1102 20-21 0.4029203028387016 1102 22-23 0.5239649065195522 1102 24-25 0.6543548335620883 1102 26-27 0.5608579497689448 1102 28-29 0.30557131759236 1102 30-31 0.5058253198985412 1102 32-33 0.3459386752965088 1102 34-35 0.25827102776756306 1102 36-37 0.41233420297288603 1102 38-39 0.37916653456154137 1102 40-41 0.44277236467173964 1102 42-43 0.5208497607929203 1102 44-45 0.46574520092092087 1102 46-47 0.42180871146806 1102 48-49 0.3529246346526733 1102 50-51 0.4198836105952566 1102 52-53 0.4573374434185524 1102 54-55 0.38938116676813905 1102 56-57 0.4624844548395046 1102 58-59 0.526844406189575 1102 60-61 0.44082273396031013 1102 62-63 0.5864643613836407 1102 64-65 0.502660646425646 1102 66-67 0.5149099983853489 1102 68-69 0.4954803163754349 1102 70-71 0.5241540667555071 1102 72-73 0.505393427807526 1102 74-75 0.47852662589644623 1102 76-77 0.4596298818815363 1102 78-79 0.6280390251408576 1102 80-81 0.6953136207023363 1102 82-83 0.5743025967316946 1102 84-85 0.6615152351830815 1102 86-87 0.580613936110943 1102 88-89 0.5095179859481291 1102 90-91 0.5538411178103289 1102 92-93 0.6391581041774721 1102 94-95 0.5742530604293599 1102 96-97 0.6520467725555079 1102 98-99 0.6898925680683909 1102 100-101 0.7576400383574651 1103 1 0.36608355554779237 1103 2 0.31673422935507745 1103 3 0.3999804632234003 1103 4 0.2975012228458027 1103 5 0.39256738457712714 1103 6 0.31954014451605417 1103 7 0.42117969442966796 1103 8 0.28515994917306386 1103 9 0.5121021142292008 1103 10-11 0.5483554210240129 1103 12-13 0.542098124953533 1103 14-15 0.5713495672711915 1103 16-17 0.6661211787012391 1103 18-19 0.5062300252313179 1103 20-21 0.4946233596072602 1103 22-23 0.6607007143798143 1103 24-25 0.7864509034310814 1103 26-27 0.6782160283715655 1103 28-29 0.6265320162823116 1103 30-31 0.7929432238286722 1103 32-33 0.6843666228947569 1103 34-35 0.5724085823527147 1103 36-37 0.5474324562479964 1103 38-39 0.507987495260231 1103 40-41 0.6308182162001188 1103 42-43 0.6144632979108238 1103 44-45 0.5721862489558589 1103 46-47 0.5571798905073564 1103 48-49 0.4787434119452456 1103 50-51 0.5784534795908982 1103 52-53 0.6481125525888558 1103 54-55 0.5272086776851737 1103 56-57 0.5200717910840496 1103 58-59 0.612543096145906 1103 60-61 0.6438795025192618 1103 62-63 0.6849905622569992 1103 64-65 0.4707283320151632 1103 66-67 0.5473881643242109 1103 68-69 0.6215720194322003 1103 70-71 0.7479531933930659 1103 72-73 0.7313759605586156 1103 74-75 0.7036903813549635 1103 76-77 0.5447827203094882 1103 78-79 0.5529844399880197 1103 80-81 0.5943310879512467 1103 82-83 0.6870209373430463 1103 84-85 0.644047986274785 1103 86-87 0.6087252898227362 1103 88-89 0.4814066322363395 1103 90-91 0.6054240872426391 1103 92-93 0.5362650910770341 1103 94-95 0.6992530604293599 1103 96-97 0.7003546328175183 1103 98-99 0.6915301226535426 1103 100-101 1.0398452785321375 1104 1 0.4457099737170722 1104 2 0.3538553253994081 1104 3 0.40419318339222343 1104 4 0.45304395190611757 1104 5 0.5071680568944146 1104 6 0.4283410355221804 1104 7 0.540581596697578 1104 8 0.4704314181724172 1104 9 0.48734030398703965 1104 10-11 0.45456718820164355 1104 12-13 0.5037142575504774 1104 14-15 0.6742228473020262 1104 16-17 0.6994983600759994 1104 18-19 0.5008566813582078 1104 20-21 0.6275490889763375 1104 22-23 0.6522620702970698 1104 24-25 0.7517807707087698 1104 26-27 0.6271661420108359 1104 28-29 0.8294333869181116 1104 30-31 0.7168044806162683 1104 32-33 0.8048490558197372 1104 34-35 0.8935113301246247 1104 36-37 0.7815997149067471 1104 38-39 0.6855447850043745 1104 40-41 0.7483501199305991 1104 42-43 0.8071731184791773 1104 44-45 0.9450986062683526 1104 46-47 1.0153676924523438 1104 48-49 0.9404502296047212 1104 50-51 0.7486854476279561 1104 52-53 0.8145171756988319 1104 54-55 0.9510784951426743 1104 56-57 0.9538934598517059 1104 58-59 0.9931388723403956 1104 60-61 0.8785104883137791 1104 62-63 0.9970828422158675 1104 64-65 1.002154841420868 1104 66-67 0.9983729605744642 1104 68-69 0.9950521377841923 1104 70-71 0.8212497941977048 1104 72-73 1.04758374629926 1104 74-75 0.8521180329571685 1104 76-77 0.969682429885129 1104 78-79 0.8240638563070384 1104 80-81 0.9067572340363199 1104 82-83 0.9781199880663216 1104 84-85 0.8099452083228904 1104 86-87 0.8202977484935623 1104 88-89 0.700889149897435 1104 90-91 1.022317684209785 1104 92-93 0.9573058247757764 1104 94-95 0.6974718607051607 1104 96-97 1.0574175627372604 1104 98-99 1.1190631813489809 1104 100-101 1.1398854121134114 1105 1 0.4337229354142451 1105 2 0.42124602436905434 1105 3 0.33244563269730065 1105 4 0.3671524554883021 1105 5 0.38298639060749196 1105 6 0.2781726853538302 1105 7 0.43831076811095926 1105 8 0.39761807167485586 1105 9 0.31090932755606104 1105 10-11 0.5326917926420407 1105 12-13 0.4635633752943349 1105 14-15 0.46585090735113965 1105 16-17 0.6178421994724701 1105 18-19 0.5002617597106465 1105 20-21 0.4307148316157665 1105 22-23 0.5219830900180895 1105 24-25 0.6052022030775746 1105 26-27 0.5753282138571123 1105 28-29 0.5484911322916446 1105 30-31 0.650646661826876 1105 32-33 0.6058642199927959 1105 34-35 0.46091595279240494 1105 36-37 0.5463006085550148 1105 38-39 0.6512241348942425 1105 40-41 0.5493690814758807 1105 42-43 0.6061516253524317 1105 44-45 0.7316343901725588 1105 46-47 0.5915214607113768 1105 48-49 0.589290765287366 1105 50-51 0.6252569268310211 1105 52-53 0.6428151934705326 1105 54-55 0.6780600905453227 1105 56-57 0.7692411725678383 1105 58-59 0.6607801978764556 1105 60-61 0.6309572618131867 1105 62-63 0.5678557340413874 1105 64-65 0.6136672081963965 1105 66-67 0.5246102710223042 1105 68-69 0.5676300472042115 1105 70-71 0.6037464398522445 1105 72-73 0.5322790704682703 1105 74-75 0.5230744644399188 1105 76-77 0.7272328716460983 1105 78-79 0.5979657828405429 1105 80-81 0.6078508772352258 1105 82-83 0.5146456456446096 1105 84-85 0.6462189735440269 1105 86-87 0.6504716681411651 1105 88-89 0.6183368094503585 1105 90-91 0.7127748878248852 1105 92-93 0.7518325456182886 1105 94-95 0.6789115710922395 1105 96-97 0.6776448987540711 1105 98-99 0.7537534423550305 1105 100-101 0.7528749566819037 1106 1 0.17116162540768087 1106 2 0.1659067434823278 1106 3 0.10310951868965645 1106 4 0.10636382535660971 1106 5 0.1591768667979636 1106 6 0.12024969458412471 1106 7 0.05681383843884902 1106 8 0.04076873015398519 1106 9 0.06758487693234372 1106 10-11 0.09688867983162908 1106 12-13 0.07637378737481271 1106 14-15 0.2199620570827321 1106 16-17 0.21683613605764407 1106 18-19 0.19784411787694722 1106 20-21 0.26530139941400677 1106 22-23 0.14314324913445375 1106 24-25 0.20252580405081488 1106 26-27 0.08672897854255268 1106 28-29 0.1812876410005657 1106 30-31 0.2175334652100247 1106 32-33 0.22446963458361324 1106 34-35 0.21410600415763525 1106 36-37 0.1607871493189279 1106 38-39 0.18238506642014585 1106 40-41 0.07746934023307261 1106 42-43 0.16559433187542538 1106 44-45 0.20132310913135143 1106 46-47 0.2779637424091277 1106 48-49 0.3048586793954158 1106 50-51 0.3702034374709626 1106 52-53 0.2339865525251028 1106 54-55 0.3016359604715504 1106 56-57 0.40388567108094975 1106 58-59 0.3339254494888664 1106 60-61 0.4367368161328855 1106 62-63 0.4123098336049722 1106 64-65 0.4655171290244269 1106 66-67 0.5442120939087971 1106 68-69 0.57333815364224 1106 70-71 0.5333046216002089 1106 72-73 0.5048450149320303 1106 74-75 0.3957968218338088 1106 76-77 0.46275984804899295 1106 78-79 0.5127419771787807 1106 80-81 0.5865149949504413 1106 82-83 0.5388162709101287 1106 84-85 0.5182423003118792 1106 86-87 0.4557143978364522 1106 88-89 0.5770399338469119 1106 90-91 0.6787116274842475 1106 92-93 0.5478372573164307 1106 94-95 0.6314354192365208 1106 96-97 0.4211643827845002 1106 98-99 0.5132173901401451 1106 100-101 0.466635432486175 1107 1 -0.0441815284057121 1107 2 0.11039229802237216 1107 3 0.15735921676581555 1107 4 0.13438032412462064 1107 5 0.1221083015603881 1107 6 0.12803023521138357 1107 7 -0.023086676385077 1107 8 0.03101904732935168 1107 9 0.23736492725110026 1107 10-11 0.09986978939750202 1107 12-13 0.19869926183866227 1107 14-15 0.17791273701860177 1107 16-17 0.011738339847489954 1107 18-19 0.12968502293673367 1107 20-21 0.17459291493046436 1107 22-23 0.2147367630534518 1107 24-25 0.21970976124710972 1107 26-27 0.1473346112156264 1107 28-29 0.3271058404838172 1107 30-31 0.16328574418426456 1107 32-33 0.20001860499225188 1107 34-35 0.204043664934197 1107 36-37 0.21849244905671128 1107 38-39 0.21364223322783715 1107 40-41 0.31950069738213926 1107 42-43 0.03788647891826713 1107 44-45 0.11628904172862065 1107 46-47 0.21429198700302265 1107 48-49 0.1414071674037629 1107 50-51 0.23287328022201592 1107 52-53 0.22279249316614624 1107 54-55 0.2920359686057097 1107 56-57 0.3160427799328289 1107 58-59 0.24687224875724212 1107 60-61 0.2532032734958136 1107 62-63 0.4273749576451209 1107 64-65 0.19752964205883217 1107 66-67 0.29047010237508886 1107 68-69 0.3543558149158841 1107 70-71 0.33282719217243795 1107 72-73 0.2704623009050309 1107 74-75 0.312797704163156 1107 76-77 0.2562803760457193 1107 78-79 0.21598558255048417 1107 80-81 0.3779053923601623 1107 82-83 0.43675895226495953 1107 84-85 0.47221741433119746 1107 86-87 0.20410027740357606 1107 88-89 0.32122966516011786 1107 90-91 0.4869713486410632 1107 92-93 0.2917561327249736 1107 94-95 0.4065583635277612 1107 96-97 0.5960573678707668 1107 98-99 0.6724060990358574 1107 100-101 0.48972540409573284 1108 1 0.06945114482666526 1108 2 0.11960302798921774 1108 3 0.2670320988627175 1108 4 0.11039946189320204 1108 5 0.19125706729921887 1108 6 0.13525984770415533 1108 7 0.2018863737918295 1108 8 0.1259945684724073 1108 9 0.22213182825224465 1108 10-11 0.045278815755381174 1108 12-13 0.002605424862700545 1108 14-15 0.08462005243607251 1108 16-17 0.2135258530508608 1108 18-19 0.05194652718488868 1108 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70-71 -2.119680878704333 2316 72-73 -2.0098892901526924 2316 74-75 -1.949083726508082 2316 76-77 -2.2855723920021767 2316 78-79 -2.346178130917462 2316 80-81 -2.2041963195628114 2316 82-83 -2.3100698314874997 2316 84-85 -2.4366205458447645 2316 86-87 -2.4247119509540305 2316 88-89 -2.3192745266622907 2316 90-91 -2.428070622353708 2316 92-93 -2.4302494025183634 2316 94-95 -2.3596952233594415 2316 96-97 -2.699337939675001 2316 98-99 -2.552249440043507 2316 100-101 -2.4455777860570898 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25066.0 3 5317.0 4 4268.0 5 3505.0 6 3346.0 7 3595.0 8 4201.0 9 4938.0 10 5691.0 11 6724.0 12 8526.0 13 11554.0 14 11577.0 15 10571.0 16 12096.0 17 14827.0 18 13762.0 19 10656.0 20 10708.0 21 11070.0 22 11731.0 23 12889.0 24 14686.0 25 16663.0 26 19543.0 27 23047.0 28 27741.0 29 33104.0 30 40660.0 31 50117.0 32 62322.0 33 81485.0 34 113326.0 35 166133.0 36 272407.0 37 440026.0 38 421761.0 39 77011.0 40 105.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.41385216087242 17.58037860649651 12.970075312738105 24.03569391989296 2 15.721336313586654 18.884766495474693 40.45882609699244 24.935071093946213 3 20.31537769932128 23.080098212566135 27.96938648115834 28.635137606954242 4 12.984137998109974 14.882342665886656 35.79385252465721 36.33966681134616 5 14.337940155029608 37.02700073987224 33.55347234285941 15.081586762238741 6 34.61573821830432 34.34099247183106 16.62676288735618 14.41650642250844 7 30.72386165356487 31.1175929214502 19.4618022120952 18.69674321288973 8 27.190037894056328 35.37585087423441 18.604428344618643 18.829682887090616 9 26.917310770815067 16.26123431841102 19.177124285484766 37.64433062528914 10-11 26.22923057159219 25.843133735050817 26.352179636276894 21.575456057080107 12-13 26.555712203918265 22.697718689595987 27.27751785763107 23.469051248854676 14-15 23.228629267668342 24.404103319969 25.0573837185811 27.309883693781558 16-17 23.355322218388295 27.68177368079998 25.67192247962225 23.29098162118947 18-19 23.277233702169173 26.417032260312357 27.50173965474023 22.803994382778242 20-21 23.875475220317874 25.633542413337768 27.594568661805713 22.89641370453865 22-23 23.460708204152716 25.565035656027845 27.5725351080133 23.401721031806137 24-25 23.330175495907515 25.74941773591526 27.709938718595552 23.210468049581678 26-27 23.26322304111921 25.992777512446768 27.588634671133903 23.155364775300118 28-29 23.023172418999614 26.219923837785807 27.712438037746224 23.044465705468358 30-31 23.34178587328933 25.888702309285534 27.580041036758868 23.189470780666266 32-33 23.082573726411816 25.924854141679464 27.695236500619774 23.29733563128894 34-35 23.441857576818276 26.04812286946066 27.481941128284394 23.02807842543667 36-37 23.522954681297538 25.912551673646423 27.44637207067212 23.11812157438392 38-39 23.248145074768203 26.071403744413836 27.528947431105383 23.151503749712578 40-41 23.36750285353784 26.012911726916126 27.556095266226876 23.06349015331916 42-43 23.378927990358104 25.988200926997095 27.254648051529117 23.378223031115684 44-45 23.29839374155594 26.080310734071805 27.308533055003053 23.312762469369204 46-47 23.45486723598124 26.35070785837435 26.961858290223027 23.232566615421383 48-49 23.614534527612292 26.285079026515294 26.929171690852055 23.171214755020355 50-51 23.388274820758657 26.397010062514088 26.91272029304072 23.301994823686535 52-53 23.48854004055848 26.521453686007323 26.690641884170034 23.29936438926416 54-55 23.416861509556075 26.304799030814145 26.802690789014626 23.475648670615147 56-57 23.4059726173797 26.570749456828143 26.71027114468319 23.31300678110897 58-59 23.617183745121736 26.3431045711831 26.729184307187314 23.310527376507846 60-61 23.492601942087443 26.3991936817007 26.991996133717365 23.11620824249449 62-63 23.302674638121896 26.466218308773804 27.00646477434549 23.224642278758814 64-65 23.261059366055054 26.462148009959623 26.911989208868352 23.36480341511697 66-67 23.399998006841397 26.533875310428378 26.61177864369446 23.454348039035768 68-69 23.23585210953758 26.402158249417756 26.918428393350485 23.44356124769418 70-71 23.34645115166407 26.288880953428546 26.879802651745628 23.484865243161764 72-73 23.389743896432503 26.42179950765958 26.746760719927536 23.44169587598039 74-75 23.259865386569874 26.741909553896676 26.618806491127483 23.379418568405963 76-77 23.413293839300387 26.60968005749066 26.508619222611955 23.468406880597 78-79 23.404438944756908 26.539852407943375 26.551667551992104 23.504041095307613 80-81 23.366486113272135 26.741238135988798 26.60256534930761 23.28971040143146 82-83 23.465041676239377 26.735201197105457 26.576616329403357 23.223140797251812 84-85 23.40231504712559 26.66809132601263 26.451086047479567 23.478507579382214 86-87 23.23500837797861 26.619142830191066 26.670708972460133 23.4751398193702 88-89 23.44676041762409 26.65918234653618 26.435309261931266 23.458747973908466 90-91 23.38516048191271 26.765729095324076 26.46253930328124 23.386571119481975 92-93 23.387441939641544 26.605067970136915 26.535881909486637 23.471608180734904 94-95 23.31530057939501 26.727390660829283 26.511308802564198 23.445999957211512 96-97 23.352074089341198 26.579215746512723 26.552212919358038 23.51649724478804 98-99 23.32388603358255 26.656525927661324 26.337454760379476 23.682133278376654 100-101 23.364095060786212 26.708551873654123 26.168597274950056 23.758755790609612 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 87.0 1 73.5 2 144.5 3 267.5 4 231.0 5 199.5 6 409.0 7 866.5 8 1397.0 9 1623.5 10 1509.0 11 1446.0 12 1565.0 13 1775.0 14 2030.5 15 2223.5 16 2435.5 17 2602.0 18 2641.0 19 2703.5 20 2901.0 21 3206.0 22 3485.5 23 4012.0 24 4852.0 25 5977.5 26 7652.5 27 9666.0 28 11750.0 29 14289.0 30 17681.0 31 21218.0 32 24709.0 33 28402.0 34 32160.5 35 35249.0 36 38892.0 37 43390.5 38 47227.5 39 50382.0 40 53218.5 41 57731.5 42 61498.5 43 65105.5 44 70358.5 45 74127.5 46 76046.0 47 77700.5 48 81502.0 49 82330.5 50 79193.0 51 77542.5 52 78023.5 53 78845.0 54 78763.5 55 76117.0 56 70421.5 57 64497.0 58 59509.0 59 53486.5 60 45930.0 61 38421.5 62 31694.0 63 25448.0 64 19575.0 65 14689.0 66 10604.5 67 8097.5 68 6054.5 69 4156.0 70 3135.0 71 2405.5 72 1868.0 73 1540.0 74 1179.5 75 883.0 76 611.0 77 315.0 78 192.0 79 128.5 80 81.5 81 57.0 82 38.5 83 31.0 84 28.0 85 27.5 86 24.5 87 21.0 88 19.0 89 20.5 90 21.0 91 17.0 92 16.5 93 16.0 94 11.0 95 16.5 96 22.5 97 19.5 98 14.5 99 11.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046885092814581315 2 0.012773647577966427 3 0.010499551228858766 4 0.0057094333871213565 5 0.008806075224204126 6 0.005661048358416939 7 0.005661048358416939 8 0.009870545855701329 9 0.01543482415670943 10-11 0.0022015188060510314 12-13 0.009749583283940284 14-15 0.008927037795965173 16-17 0.019741091711402658 18-19 0.012241412262217826 20-21 0.003967572353762299 22-23 0.009870545855701329 24-25 0.022620000919315545 26-27 0.007451294420480415 28-29 0.018192770792861274 30-31 0.015241284041891758 32-33 0.013572000551589327 34-35 0.2666257006756969 36-37 0.4710040619231597 38-39 0.4675929173994982 40-41 0.4885678273428636 42-43 0.47893920663068434 44-45 0.4937934104429408 46-47 0.4986319133133825 48-49 0.5056961275042277 50-51 0.5157118284460421 52-53 0.5063493253917373 54-55 0.4927289398114435 56-57 0.4879872069984106 58-59 0.486535656137278 60-61 0.4838019020154784 62-63 0.47978594463301166 64-65 0.496019121763344 66-67 0.4702299014638891 68-69 0.4970835923948412 70-71 0.47821343120011806 72-73 0.48617276842199486 74-75 0.5006882770333203 76-77 0.4873823941396053 78-79 0.4873582016252531 80-81 0.4894145653451909 82-83 0.4900193782039961 84-85 0.48980164557482625 86-87 0.4922208970100471 88-89 0.5059138601333976 90-91 0.5296709092272669 92-93 0.5179617322807977 94-95 0.4899951856896439 96-97 0.46270602950035206 98-99 0.45370641416133023 100-101 0.4618350989836725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2066755.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.06085414148059 #Duplication Level Percentage of deduplicated Percentage of total 1 75.38042577751011 43.01271480413208 2 13.660086531650926 15.589124102850738 3 4.652218345677009 7.9637865737098785 4 2.122324448972937 4.844065832949716 5 1.191695752054655 3.3999588744506344 6 0.7188619315532704 2.4611325494534495 7 0.4647897451673053 1.8564909898813275 8 0.32092879967094967 1.46499771422596 9 0.2286839215228089 1.1744009901463313 >10 1.1624454441012428 11.740132183385667 >50 0.06793092994418083 2.6293577658670464 >100 0.02765283289958173 2.656539232703085 >500 0.0016154454881292916 0.6184705576788869 >1k 3.400937869745676E-4 0.5888278285651327 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4711 0.2279418702265145 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3469 0.167847664575627 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2381 0.11520475334521993 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.8385028704418276E-5 2 0.0 0.0 0.0 0.0 4.8385028704418276E-5 3 0.0 0.0 0.0 0.0 4.8385028704418276E-5 4 0.0 0.0 0.0 0.0 9.677005740883655E-5 5 0.0 0.0 0.0 0.0 9.677005740883655E-5 6 0.0 0.0 0.0 0.0 9.677005740883655E-5 7 0.0 0.0 0.0 0.0 9.677005740883655E-5 8 0.0 0.0 0.0 0.0 9.677005740883655E-5 9 0.0 0.0 0.0 0.0 9.677005740883655E-5 10-11 0.0 0.0 0.0 0.0 9.677005740883655E-5 12-13 4.8385028704418276E-5 0.0 0.0 0.0 9.677005740883655E-5 14-15 4.8385028704418276E-5 0.0 0.0 2.4192514352209138E-5 9.677005740883655E-5 16-17 4.8385028704418276E-5 0.0 0.0 9.677005740883655E-5 9.677005740883655E-5 18-19 9.677005740883655E-5 0.0 0.0 2.1773262916988226E-4 9.677005740883655E-5 20-21 9.677005740883655E-5 0.0 0.0 4.5965777269197364E-4 9.677005740883655E-5 22-23 9.677005740883655E-5 0.0 0.0 8.467380023273199E-4 9.677005740883655E-5 24-25 9.677005740883655E-5 0.0 0.0 0.0011128556602016204 9.677005740883655E-5 26-27 9.677005740883655E-5 0.0 0.0 0.0014515508611325483 9.677005740883655E-5 28-29 1.4515508611325485E-4 0.0 0.0 0.0020563637199377768 9.677005740883655E-5 30-31 1.4515508611325485E-4 0.0 0.0 0.0030482568083783513 9.677005740883655E-5 32-33 1.4515508611325485E-4 0.0 0.0 0.004838502870441828 9.677005740883655E-5 34-35 1.4515508611325485E-4 0.0 0.0 0.007136791733901696 9.677005740883655E-5 36-37 1.4515508611325485E-4 0.0 0.0 0.012265604776570033 9.677005740883655E-5 38-39 1.4515508611325485E-4 0.0 0.0 0.02191841800310148 9.677005740883655E-5 40-41 1.4515508611325485E-4 0.0 0.0 0.03461948803801128 9.677005740883655E-5 42-43 1.4515508611325485E-4 4.8385028704418276E-5 0.0 0.04475615155158691 9.677005740883655E-5 44-45 2.4192514352209139E-4 4.8385028704418276E-5 0.0 0.05762656918696217 9.677005740883655E-5 46-47 2.903101722265097E-4 4.8385028704418276E-5 0.0 0.07429521157563426 9.677005740883655E-5 48-49 2.903101722265097E-4 4.8385028704418276E-5 0.0 0.09430242094491123 9.677005740883655E-5 50-51 2.903101722265097E-4 4.8385028704418276E-5 0.0 0.11820462512489385 9.677005740883655E-5 52-53 3.145026865787188E-4 4.8385028704418276E-5 0.0 0.1395424227835423 1.2096257176104569E-4 54-55 3.3869520093092795E-4 4.8385028704418276E-5 0.0 0.16218661621721006 1.4515508611325485E-4 56-57 3.3869520093092795E-4 4.8385028704418276E-5 0.0 0.18940319486344537 1.4515508611325485E-4 58-59 3.3869520093092795E-4 4.8385028704418276E-5 0.0 0.2225953245546763 1.4515508611325485E-4 60-61 3.3869520093092795E-4 4.8385028704418276E-5 0.0 0.2585937859107635 1.4515508611325485E-4 62-63 3.3869520093092795E-4 4.8385028704418276E-5 0.0 0.29609218315668767 1.4515508611325485E-4 64-65 3.3869520093092795E-4 4.8385028704418276E-5 0.0 0.335695329151254 1.4515508611325485E-4 66-67 3.3869520093092795E-4 4.8385028704418276E-5 0.0 0.38311265728158395 1.4515508611325485E-4 68-69 3.3869520093092795E-4 4.8385028704418276E-5 0.0 0.4380780498898031 1.4515508611325485E-4 70-71 3.628877152831371E-4 4.8385028704418276E-5 0.0 0.5011479348060123 1.935401148176731E-4 72-73 4.1127274398755533E-4 4.8385028704418276E-5 0.0 0.5655484080115931 1.935401148176731E-4 74-75 4.354652583397645E-4 4.8385028704418276E-5 0.0 0.6322713625949858 1.935401148176731E-4 76-77 4.354652583397645E-4 4.8385028704418276E-5 0.0 0.7036392799340028 1.935401148176731E-4 78-79 4.354652583397645E-4 4.8385028704418276E-5 0.0 0.7915306845755786 1.935401148176731E-4 80-81 4.354652583397645E-4 4.8385028704418276E-5 0.0 0.8941553304576497 1.935401148176731E-4 82-83 4.5965777269197364E-4 4.8385028704418276E-5 0.0 0.9999733882342126 2.4192514352209139E-4 84-85 4.8385028704418277E-4 4.8385028704418276E-5 0.0 1.1061301412117062 2.4192514352209139E-4 86-87 5.08042801396392E-4 4.8385028704418276E-5 0.0 1.2168108943730631 2.4192514352209139E-4 88-89 5.32235315748601E-4 4.8385028704418276E-5 0.0 1.3442328674661486 2.4192514352209139E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6920 0.0 31.889626 1 GGTATCA 4735 0.0 30.003428 1 GTATAGG 570 0.0 29.90868 1 GTACATG 7665 0.0 29.099022 1 TACATGG 8080 0.0 27.47859 2 TCAACGC 8055 0.0 26.9742 4 ATCAACG 8090 0.0 26.8588 3 ACATGGG 7930 0.0 26.564964 3 CAACGCA 8250 0.0 26.164492 5 TATCAAC 8325 0.0 26.158121 2 AACGCAG 8480 0.0 25.621069 6 GTACCTG 1800 0.0 24.203876 1 CATGGGG 4555 0.0 23.278826 4 ATACCGT 165 2.5061709E-6 22.9502 6 TATAACG 190 3.397945E-7 22.424454 2 AGTACAT 5495 0.0 22.313362 2 TACCTGG 1830 0.0 22.247454 2 ACGCAGA 9710 0.0 22.22985 7 GAGTACA 6220 0.0 22.002754 1 CGCAGAG 10000 0.0 21.538889 8 >>END_MODULE