FastQCFastQC Report
Thu 26 May 2016
SRR936351_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936351_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066755
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51840.2508279888037044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38370.18565335513885292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24770.11984971610084408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78000.031.5910431
GGTATCA51650.031.0376191
GTACATG82100.027.5798951
TCAACGC92000.026.5042324
ATCAACG91950.026.4670523
TACATGG81750.026.4093762
CAACGCA93050.026.2561345
TATCAAC94750.025.8407442
ACATGGG82900.025.5795523
GTACCTG19350.025.5670831
AACGCAG95150.025.5270796
TACCTGG19600.023.4828172
CTCGCGC3700.023.07950460-61
ATTCGCG4050.022.8719480-81
ACGCAGA109000.022.1529337
CGCGATC4200.022.02707322-23
TCGCGCT4100.021.9849560-61
CATGGGG49500.021.8509124
CGCGGAA3850.021.71337582-83
CGCAGAG111300.021.5246528
Produced by FastQC (version 0.11.5)