##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936351_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2066755 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47827875098887 34.0 31.0 34.0 31.0 34.0 2 32.60998521837373 34.0 31.0 34.0 31.0 34.0 3 32.69499481070567 34.0 31.0 34.0 31.0 34.0 4 36.179437330501194 37.0 35.0 37.0 35.0 37.0 5 36.142897440673906 37.0 35.0 37.0 35.0 37.0 6 36.178619139665805 37.0 37.0 37.0 35.0 37.0 7 36.13314834124025 37.0 36.0 37.0 35.0 37.0 8 36.10874680356404 37.0 36.0 37.0 35.0 37.0 9 37.87723750517115 39.0 38.0 39.0 35.0 39.0 10-11 37.808713901744525 39.0 38.0 39.0 35.0 39.0 12-13 37.753298528369356 39.0 38.0 39.0 35.0 39.0 14-15 39.09981492726521 40.0 38.5 41.0 36.0 41.0 16-17 39.03025298112258 40.0 38.0 41.0 36.0 41.0 18-19 39.052526303311225 40.0 38.5 41.0 36.0 41.0 20-21 39.003751775125735 40.0 38.5 41.0 35.0 41.0 22-23 38.88958294524508 40.0 38.0 41.0 35.0 41.0 24-25 39.04181797068351 40.0 39.0 41.0 35.0 41.0 26-27 38.96412177544025 40.0 38.5 41.0 35.0 41.0 28-29 38.87065762511763 40.0 38.0 41.0 35.0 41.0 30-31 38.758255332634974 40.0 38.0 41.0 35.0 41.0 32-33 38.6066236685045 40.0 38.0 41.0 34.0 41.0 34-35 38.47365531957102 40.0 38.0 41.0 34.0 41.0 36-37 38.330994481687476 40.0 38.0 41.0 34.0 41.0 38-39 38.19932769002615 40.0 38.0 41.0 33.5 41.0 40-41 38.057412707360086 40.0 38.0 41.0 33.0 41.0 42-43 38.08465613969726 40.0 38.0 41.0 33.0 41.0 44-45 37.95081057019337 40.0 38.0 41.0 33.0 41.0 46-47 37.75114708806801 40.0 37.0 41.0 33.0 41.0 48-49 37.52258226059693 40.0 37.0 41.0 33.0 41.0 50-51 37.32127465519619 40.0 37.0 41.0 32.0 41.0 52-53 37.112623895914126 40.0 36.0 41.0 31.5 41.0 54-55 36.91533128019528 39.5 36.0 41.0 31.0 41.0 56-57 36.65941584754845 39.0 35.5 41.0 31.0 41.0 58-59 36.390465488168644 39.0 35.0 41.0 31.0 41.0 60-61 36.10860406772936 38.5 35.0 40.5 30.0 41.0 62-63 35.806430612239964 38.0 35.0 40.0 30.0 41.0 64-65 35.431412528335485 37.0 35.0 40.0 29.0 41.0 66-67 35.07494768368771 37.0 34.0 40.0 29.0 41.0 68-69 34.71309202106684 36.0 34.0 39.0 28.5 41.0 70-71 34.332372971155266 36.0 34.0 39.0 28.0 40.5 72-73 33.91535837581136 35.0 34.0 38.5 28.0 40.0 74-75 33.48668444010055 35.0 33.5 37.0 27.0 39.5 76-77 32.76930163468819 35.0 32.5 36.5 26.0 39.0 78-79 32.81587875679507 35.0 33.0 36.0 26.0 39.0 80-81 32.6406066514899 35.0 33.0 36.0 26.0 37.5 82-83 32.35654298646912 35.0 33.0 35.5 26.0 37.0 84-85 32.06816289303764 35.0 33.0 35.0 26.0 37.0 86-87 31.6606857126268 35.0 32.0 35.0 25.0 36.0 88-89 31.167267044231174 35.0 32.0 35.0 23.5 36.0 90-91 30.94354725160941 34.0 32.0 35.0 22.0 36.0 92-93 30.653075957237313 34.0 31.0 35.0 20.0 35.0 94-95 30.485417720049064 34.0 31.0 35.0 19.0 35.0 96-97 30.226928203875154 34.0 31.0 35.0 16.5 35.0 98-99 29.76780726307666 34.0 31.0 35.0 2.0 35.0 100-101 26.3877970538356 31.0 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11253868051734628 1101 2 0.0802776842108841 1101 3 0.15609278826181594 1101 4 0.13866154695878663 1101 5 0.012322214675123178 1101 6 0.17375007444671553 1101 7 0.08372151252505233 1101 8 0.051387487831171086 1101 9 0.105620599811175 1101 10-11 0.06589427248997026 1101 12-13 0.24532194034864574 1101 14-15 0.2608939462536952 1101 16-17 0.34930525661979317 1101 18-19 0.3068840695712822 1101 20-21 0.2385529024414339 1101 22-23 0.2196508155953154 1101 24-25 0.15166974104612763 1101 26-27 0.29962768563466824 1101 28-29 0.23842540642672105 1101 30-31 0.2161778586351275 1101 32-33 0.21467915690060835 1101 34-35 0.18855280419946752 1101 36-37 0.25428177250019957 1101 38-39 0.30945838431430417 1101 40-41 0.3398012381607032 1101 42-43 0.3182106470542223 1101 44-45 0.3249401703336616 1101 46-47 0.28884896779704405 1101 48-49 0.2663282555261688 1101 50-51 0.1822717193307284 1101 52-53 0.3461706091472081 1101 54-55 0.2857970603115092 1101 56-57 0.05477096169494189 1101 58-59 0.08785320890374493 1101 60-61 0.2775114348611609 1101 62-63 0.3076956757159124 1101 64-65 0.27253270916907013 1101 66-67 0.24293718797176211 1101 68-69 0.40638379863902685 1101 70-71 0.347939594908965 1101 72-73 0.33734434012907855 1101 74-75 0.2771267977519898 1101 76-77 0.2200645537932715 1101 78-79 0.22798004961509832 1101 80-81 0.38141944572196707 1101 82-83 0.4279147235589349 1101 84-85 0.4241777802320499 1101 86-87 0.2077018003305362 1101 88-89 0.20749690340758065 1101 90-91 0.4279113996179227 1101 92-93 0.44470625110212936 1101 94-95 0.45468516204068266 1101 96-97 0.32715952107635715 1101 98-99 0.2918199154356351 1101 100-101 0.4256604718727388 1102 1 -0.014507957428001816 1102 2 0.08735665805885162 1102 3 0.04298307088346576 1102 4 0.029140772850958285 1102 5 -0.015751894133721578 1102 6 0.09135580112530306 1102 7 0.10500117412153287 1102 8 0.0706144057605016 1102 9 0.04989001356418754 1102 10-11 0.1304744919051899 1102 12-13 0.11778928694849355 1102 14-15 0.16281712650437186 1102 16-17 0.220890936992582 1102 18-19 0.2289836074902638 1102 20-21 0.2531071114818104 1102 22-23 0.25142621295568546 1102 24-25 0.13573747059684393 1102 26-27 0.13745299849184534 1102 28-29 0.12996397505015267 1102 30-31 0.1843102648756627 1102 32-33 0.15921050611857623 1102 34-35 0.04913108932202448 1102 36-37 0.06746194990804355 1102 38-39 0.14421679311435298 1102 40-41 0.11977727930838 1102 42-43 0.06867712419928296 1102 44-45 0.12022264544673789 1102 46-47 0.23205232252482944 1102 48-49 0.1256115170562211 1102 50-51 0.15441497421774386 1102 52-53 0.20198765241479322 1102 54-55 0.08893271479603015 1102 56-57 0.16595676613600574 1102 58-59 0.019746935152490153 1102 60-61 -0.014020905926038552 1102 62-63 0.13068957976327766 1102 64-65 0.0673847308956681 1102 66-67 0.022541390662190963 1102 68-69 -0.015881814912738434 1102 70-71 0.03967513411746637 1102 72-73 -0.055859258827574365 1102 74-75 0.015889216790128557 1102 76-77 -0.09491436925509333 1102 78-79 -0.11859659428942848 1102 80-81 0.14011643584535705 1102 82-83 0.09296886909980628 1102 84-85 0.13376217596097462 1102 86-87 -0.0158940057712762 1102 88-89 0.0655639051562602 1102 90-91 0.28124707754842504 1102 92-93 0.1995138964427312 1102 94-95 0.1073208800247194 1102 96-97 -0.04600937295762364 1102 98-99 -0.05412600740757867 1102 100-101 0.3509978740590789 1103 1 -0.018328918126691462 1103 2 0.049692902600341426 1103 3 0.049533289224079624 1103 4 0.0029398994885099228 1103 5 -0.014114339548569887 1103 6 0.04823353038294442 1103 7 0.09190073744030514 1103 8 0.08753580314041187 1103 9 0.09246643277816702 1103 10-11 0.1176469809881624 1103 12-13 0.09404474546377628 1103 14-15 0.07766428807642711 1103 16-17 0.190869102931444 1103 18-19 0.04366701360379466 1103 20-21 0.14448265733333443 1103 22-23 0.09367512125263744 1103 24-25 0.10244052736540255 1103 26-27 0.14673247447438342 1103 28-29 0.20747489208071812 1103 30-31 0.20941943518133854 1103 32-33 0.22553146681727299 1103 34-35 0.19350881857966584 1103 36-37 0.2355842206504022 1103 38-39 0.15595260097461505 1103 40-41 0.21693885136078706 1103 42-43 0.24908105433028283 1103 44-45 0.2051025581104966 1103 46-47 0.28363529195713966 1103 48-49 0.32402854762390376 1103 50-51 0.37002632792953705 1103 52-53 0.2120859056898965 1103 54-55 0.4352755095558649 1103 56-57 0.31497423338490904 1103 58-59 0.2127054504363315 1103 60-61 0.2398000547726582 1103 62-63 0.2158424181912224 1103 64-65 0.187199141376027 1103 66-67 0.12379684917747369 1103 68-69 0.13286272657197173 1103 70-71 0.014020112283411379 1103 72-73 0.09998135252613594 1103 74-75 0.19410974080759047 1103 76-77 0.2170397792165275 1103 78-79 0.06562829654026103 1103 80-81 0.18023652318159833 1103 82-83 0.30503218787709585 1103 84-85 0.25985387901774715 1103 86-87 0.23437891999291693 1103 88-89 0.2596141234968705 1103 90-91 0.44391083300694234 1103 92-93 0.476806472861945 1103 94-95 0.36605450447886767 1103 96-97 0.24329193708604535 1103 98-99 0.1713106738151282 1103 100-101 0.38265726270536504 1104 1 -0.013212829638966639 1104 2 0.060294204054315514 1104 3 0.03287277463205385 1104 4 0.03980562639675611 1104 5 0.12571506226083784 1104 6 0.07130206913753057 1104 7 0.025151888342570317 1104 8 0.1096350999466722 1104 9 0.07680185781687499 1104 10-11 0.11090683185258143 1104 12-13 0.17389591749203248 1104 14-15 0.1823158659094588 1104 16-17 0.2036233403179537 1104 18-19 0.2285303272737096 1104 20-21 0.12344473849893944 1104 22-23 0.11263889133683591 1104 24-25 0.180360035013706 1104 26-27 0.2046265438744257 1104 28-29 0.2559121780639657 1104 30-31 0.11819476025463871 1104 32-33 0.12080951737571866 1104 34-35 0.15779529980191143 1104 36-37 0.051940004413104646 1104 38-39 0.14165352541088794 1104 40-41 0.26997858563758825 1104 42-43 0.29084943336308555 1104 44-45 0.3552075206292642 1104 46-47 0.3207736544373816 1104 48-49 0.3941041601035593 1104 50-51 0.4809508454559861 1104 52-53 0.4627331793922238 1104 54-55 0.4099215621293979 1104 56-57 0.4229792722822765 1104 58-59 0.36001049351246195 1104 60-61 0.2901484014896525 1104 62-63 0.37959178267030325 1104 64-65 0.35239482378039355 1104 66-67 0.3547351392257383 1104 68-69 0.4864617772952542 1104 70-71 0.4383005517040388 1104 72-73 0.36150996315552675 1104 74-75 0.3873847106856374 1104 76-77 0.35023770886929384 1104 78-79 0.3241046656090276 1104 80-81 0.43571549701555057 1104 82-83 0.3796481475194895 1104 84-85 0.371735305324016 1104 86-87 0.27245387779897356 1104 88-89 0.3608549273060504 1104 90-91 0.5228973773750063 1104 92-93 0.4848578554536651 1104 94-95 0.4669122545839173 1104 96-97 0.40818480392961476 1104 98-99 0.24162438899496763 1104 100-101 0.35027486019253473 1105 1 -0.019212677744079087 1105 2 0.042745281242240196 1105 3 -0.00506803699296654 1105 4 0.043453635307926675 1105 5 -0.01107894295422085 1105 6 0.10286835859855614 1105 7 0.07461139569682018 1105 8 0.15084418326102167 1105 9 0.09544695540934356 1105 10-11 0.09110733310571817 1105 12-13 0.13845251099599665 1105 14-15 0.16072527326623032 1105 16-17 0.20477647568161217 1105 18-19 0.12756959210244645 1105 20-21 0.12324094618987402 1105 22-23 0.0640553111743074 1105 24-25 0.10848590524484081 1105 26-27 0.1880522799317461 1105 28-29 0.08690379852927066 1105 30-31 0.08951953684257319 1105 32-33 0.16871843107410456 1105 34-35 0.2225847988019325 1105 36-37 0.11847249726138642 1105 38-39 0.05813918400181706 1105 40-41 0.19073249060202357 1105 42-43 0.17885480031582546 1105 44-45 0.06211747227605713 1105 46-47 0.029286097160344582 1105 48-49 0.10999236546545177 1105 50-51 0.10507808274111596 1105 52-53 0.22438604893982728 1105 54-55 0.12854767286077617 1105 56-57 0.17344128590217878 1105 58-59 0.2502196021426215 1105 60-61 0.21246935538955825 1105 62-63 0.2751311361043989 1105 64-65 0.23084473644082948 1105 66-67 0.24546195626834333 1105 68-69 0.2348921967782971 1105 70-71 0.17390724520547707 1105 72-73 0.24423951430613045 1105 74-75 0.3220053532010212 1105 76-77 0.12204601751970046 1105 78-79 0.02333000167646304 1105 80-81 0.13232317783375436 1105 82-83 0.09507054188924968 1105 84-85 0.1975878153870525 1105 86-87 0.0898686267926685 1105 88-89 0.16034481363804076 1105 90-91 0.3346059417151501 1105 92-93 0.40024227610124186 1105 94-95 0.3072960423361266 1105 96-97 0.3371422986765822 1105 98-99 0.204736715265188 1105 100-101 0.470673077959173 1106 1 -9.554183861837373E-5 1106 2 -0.030610451967511665 1106 3 -0.0034034944525132005 1106 4 -0.021015526241527027 1106 5 0.035284939176086993 1106 6 0.06628704318512746 1106 7 0.12043072910739028 1106 8 0.02741700216960652 1106 9 -0.016011739990958063 1106 10-11 0.1358703964819128 1106 12-13 0.13980036562851694 1106 14-15 0.09235930563018968 1106 16-17 0.011475197343841614 1106 18-19 0.059564725922399475 1106 20-21 0.05817123622966136 1106 22-23 -0.021525733895792598 1106 24-25 0.046559902077959237 1106 26-27 -0.08317546501863404 1106 28-29 0.017454526525256142 1106 30-31 0.004693522089553426 1106 32-33 -0.033299488827019275 1106 34-35 -0.07623148359099474 1106 36-37 -0.1935925396211715 1106 38-39 -0.17260078432939707 1106 40-41 -0.2211394438976484 1106 42-43 -0.184691331843446 1106 44-45 -0.18498406095685738 1106 46-47 -0.2296680603339567 1106 48-49 -0.336514412428194 1106 50-51 -0.24606701438938217 1106 52-53 -0.3519241507280384 1106 54-55 -0.2824939213925006 1106 56-57 -0.25905012104178127 1106 58-59 -0.17924782148538299 1106 60-61 -0.1366906492449047 1106 62-63 -0.21005211805696433 1106 64-65 -0.16857595641052114 1106 66-67 -0.3397657114410748 1106 68-69 -0.22682525581506496 1106 70-71 -0.3756273780517745 1106 72-73 -0.4301261443660991 1106 74-75 -0.28631929052508553 1106 76-77 -0.4374430335751853 1106 78-79 -0.5768437904681321 1106 80-81 -0.44944988824587995 1106 82-83 -0.35896915996067236 1106 84-85 -0.2766040511405805 1106 86-87 -0.2751470145003445 1106 88-89 -0.41646938324915084 1106 90-91 -0.2844378152848108 1106 92-93 -0.1639179216355977 1106 94-95 -0.18854260131273648 1106 96-97 -0.20506988222173206 1106 98-99 -0.03386831312085903 1106 100-101 0.23945035367075462 1107 1 -0.08478560247193201 1107 2 0.0147820603212665 1107 3 -0.028835141041760437 1107 4 -0.04189148384001129 1107 5 -0.03632156678839493 1107 6 -0.05594663965305813 1107 7 -0.02798079562776934 1107 8 0.012790218446490087 1107 9 9.999313848538804E-4 1107 10-11 -0.07395311505332103 1107 12-13 -0.02544913248311076 1107 14-15 0.07961439145112337 1107 16-17 0.12860950233154256 1107 18-19 0.036737419580134656 1107 20-21 0.0702956390192142 1107 22-23 0.03666704998322956 1107 24-25 8.411764733509131E-4 1107 26-27 0.12938202478261474 1107 28-29 -0.04606782035784107 1107 30-31 -0.1351237645612855 1107 32-33 -0.14379711806116546 1107 34-35 0.009376775734757814 1107 36-37 -0.1275847579507925 1107 38-39 0.016245657601245966 1107 40-41 -0.04300268397822293 1107 42-43 -0.16647902600391973 1107 44-45 -0.14455733439874052 1107 46-47 -0.2057460329422085 1107 48-49 -0.36378104422345103 1107 50-51 -0.24831463825724143 1107 52-53 -0.2911693919677276 1107 54-55 -0.13920069770449572 1107 56-57 -0.1984281070939744 1107 58-59 -0.27989154909528935 1107 60-61 -0.15170973625432538 1107 62-63 -0.02045806863973354 1107 64-65 0.043410730696969324 1107 66-67 -0.13217824390706312 1107 68-69 -0.07738029859278583 1107 70-71 0.009813566323067846 1107 72-73 -0.10204847001002548 1107 74-75 -0.06338906458776705 1107 76-77 -0.3206795667551745 1107 78-79 -0.21777302675050692 1107 80-81 -0.002234309540639856 1107 82-83 0.18769007676230842 1107 84-85 0.07032423389811981 1107 86-87 0.14780083542910205 1107 88-89 0.2396766450825467 1107 90-91 -0.08923826311496796 1107 92-93 0.2527536405844373 1107 94-95 0.1227815479624752 1107 96-97 -0.20939905772393175 1107 98-99 -0.1265518709529765 1107 100-101 -0.043810977369950876 1108 1 -0.12898711383430594 1108 2 -0.04903973685856755 1108 3 -0.06535764991416215 1108 4 0.017416319796922153 1108 5 0.04843094287145533 1108 6 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2316 62-63 -0.7568026282010436 2316 64-65 -0.677015944986735 2316 66-67 -0.7589877216920087 2316 68-69 -0.631064872532086 2316 70-71 -0.5823788219460866 2316 72-73 -0.7841427940312187 2316 74-75 -0.7715922132140776 2316 76-77 -0.6103363621837659 2316 78-79 -0.6589194586172056 2316 80-81 -0.8146799404694747 2316 82-83 -0.9241743252012498 2316 84-85 -0.7947581675921249 2316 86-87 -0.8353628670787678 2316 88-89 -0.685655313235074 2316 90-91 -0.8846294098918754 2316 92-93 -0.8366597600139798 2316 94-95 -0.6651645625710856 2316 96-97 -0.617377159495927 2316 98-99 -0.769826018533216 2316 100-101 -0.6749333457851279 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 11.0 5 45.0 6 68.0 7 148.0 8 326.0 9 652.0 10 1004.0 11 1663.0 12 2530.0 13 3801.0 14 4806.0 15 6529.0 16 8400.0 17 11404.0 18 10734.0 19 7248.0 20 6518.0 21 7111.0 22 7490.0 23 8602.0 24 10161.0 25 12376.0 26 15038.0 27 19027.0 28 23694.0 29 29533.0 30 36598.0 31 45470.0 32 57363.0 33 74274.0 34 103512.0 35 154350.0 36 263352.0 37 486707.0 38 544388.0 39 101695.0 40 117.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.6087447160609 18.04192608282849 13.401180574995427 23.948148626115188 2 15.981363907597737 19.266829371929617 39.94423884474424 24.807567875728413 3 20.322292676200128 23.142704384409377 28.27398506354164 28.261017875848854 4 13.110455762777882 15.172286990959257 35.80598571190102 35.91127153436184 5 14.283599168745207 37.00007983529736 33.67114147540468 15.04517952055275 6 34.60206942767769 34.59021509564511 16.453812861224478 14.353902615452727 7 30.58567657995263 31.177183555864147 19.475457903815403 18.761681960367824 8 27.119905358883855 35.56096392654185 18.497354548555585 18.82177616601871 9 26.64171612019809 16.232112659700835 19.208179005252195 37.91799221484888 10-11 26.13520293988857 25.95470171695105 26.33764687951344 21.572448463646943 12-13 26.522024391872474 22.700645553365327 27.33738509759749 23.439944957164712 14-15 23.051474412787197 24.454180587442632 25.196673045426284 27.297671954343887 16-17 23.194361383197176 27.747819279370294 25.79303330643117 23.26478603100136 18-19 23.097198264912873 26.47290075504837 27.638544481566512 22.791356498472243 20-21 23.686310181903515 25.58339038734635 27.789771888782173 22.940527541967963 22-23 23.336400945637457 25.502285226011416 27.794406107354817 23.366907720996306 24-25 23.251772571787736 25.74122375254663 27.872253381658346 23.134750294007283 26-27 23.19191871294365 26.00236463870793 27.745844160777043 23.059872487571372 28-29 22.8816118054813 26.190943924641253 27.91151211520196 23.015932154675493 30-31 23.224521424287577 25.856938842805654 27.736984702657313 23.181555030249452 32-33 22.990535728110242 25.875735464891232 27.860160060385535 23.27356874661299 34-35 23.364013201601185 25.96874563015098 27.663509165977572 23.003732002270265 36-37 23.38463362207134 25.922745950470606 27.56808084180375 23.124539585654304 38-39 23.132219814525357 26.014907936440352 27.73487765703811 23.117994591996183 40-41 23.269537970091523 25.95427529897953 27.701351670760477 23.07483506016847 42-43 23.26431823894776 25.953519024654838 27.372272398131585 23.409890338265818 44-45 23.21806466410005 26.021011151060076 27.478190098102523 23.28273408673735 46-47 23.363902563663313 26.306233325929355 27.11370234173757 23.216161768669757 48-49 23.507769109677305 26.283951292383616 26.992218066416225 23.216061531522854 50-51 23.35480967669353 26.36221550700058 27.01990936870196 23.263065447603932 52-53 23.438294256565573 26.484949030881484 26.807460292766677 23.269296419786272 54-55 23.316896883412973 26.27214348338549 26.9229001310271 23.48805950217443 56-57 23.344924300652902 26.51043658826373 26.84379484060424 23.30084427047913 58-59 23.58477510994469 26.271615762359797 26.84527809994673 23.298331027748787 60-61 23.40609346441044 26.325474271461214 27.172757832752097 23.09567443137625 62-63 23.24513549017663 26.38229978879935 27.083713357412947 23.28885136361107 64-65 23.26468313403736 26.418742426152185 26.93852400418341 23.37805043562704 66-67 23.287133695092066 26.459788412269475 26.79504827616239 23.458029616476068 68-69 23.133414458898127 26.39739591775513 27.04452148416237 23.424668139184373 70-71 23.35019473797406 26.302871922434797 26.927402738065027 23.419530601526116 72-73 23.326769820032 26.400685914961734 26.821447519594372 23.451096745411892 74-75 23.220241392908207 26.634990601208173 26.71589036920196 23.42887763668166 76-77 23.35384744414735 26.53534048260882 26.610411026376983 23.500401046866852 78-79 23.285970996522295 26.53505797789381 26.689423514710015 23.48954751087388 80-81 23.311579098128245 26.71972648500719 26.658058840948286 23.31063557591628 82-83 23.40753983902301 26.714366240797773 26.635861531724853 23.242232388454365 84-85 23.291119518875252 26.605226926167074 26.568187926834547 23.535465628123127 86-87 23.180305719345405 26.518962306951295 26.761839315555296 23.538892658148004 88-89 23.36222576002624 26.620666561862873 26.51926159075611 23.497846087354773 90-91 23.353766854014385 26.66203150936253 26.56042206626091 23.423779570362175 92-93 23.339514933258858 26.603186172756587 26.581688472112795 23.47561042187175 94-95 23.297661345303457 26.581034218302502 26.63019469192689 23.49110974446715 96-97 23.216824700446292 26.527272107047978 26.689584044511548 23.56631914799418 98-99 23.226149059607394 26.58340147772525 26.45062878322342 23.739820679443945 100-101 23.15238746596998 26.585448639803282 26.46431592920547 23.797847965021266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 101.0 1 77.0 2 117.5 3 223.5 4 192.0 5 159.0 6 360.5 7 787.5 8 1330.5 9 1586.0 10 1355.0 11 1120.0 12 1177.5 13 1350.0 14 1497.0 15 1600.5 16 1675.5 17 1724.0 18 1820.0 19 2034.0 20 2313.0 21 2667.0 22 3146.5 23 3876.5 24 4851.5 25 6068.0 26 7720.0 27 9804.5 28 11939.0 29 14586.5 30 17800.0 31 21350.0 32 25213.0 33 28791.0 34 32326.5 35 35411.5 36 39309.0 37 43734.0 38 47364.0 39 50735.5 40 53833.0 41 57995.5 42 62065.0 43 66080.5 44 71044.0 45 74635.0 46 76533.5 47 78515.0 48 82601.5 49 83068.0 50 79496.5 51 77743.5 52 78196.5 53 79311.5 54 79313.5 55 76396.5 56 70586.5 57 64325.0 58 59307.5 59 53506.5 60 45876.5 61 38226.5 62 31333.5 63 24952.5 64 19129.0 65 14340.5 66 10110.5 67 7762.5 68 5793.5 69 3868.0 70 2893.0 71 2224.0 72 1733.0 73 1486.0 74 1181.5 75 859.0 76 557.0 77 259.5 78 155.5 79 89.5 80 53.0 81 35.0 82 17.0 83 6.0 84 4.5 85 2.0 86 1.0 87 2.0 88 2.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.268827219481748 2 0.02138618268735288 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.8385028704418277E-4 12-13 4.8385028704418276E-5 14-15 0.0 16-17 3.3869520093092795E-4 18-19 0.0 20-21 0.0 22-23 4.8385028704418276E-5 24-25 0.002636984064390796 26-27 0.002636984064390796 28-29 0.0026127915500385873 30-31 0.0011370481745538296 32-33 0.00169347600465464 34-35 0.001741861033359058 36-37 1.2096257176104569E-4 38-39 1.6934760046546398E-4 40-41 7.983529736229017E-4 42-43 0.00394337983941009 44-45 0.004378845097749854 46-47 0.014418738553916647 48-49 0.014612278668734319 50-51 0.00677390401861856 52-53 0.007765797107059134 54-55 3.3869520093092795E-4 56-57 0.0028789092079128877 58-59 0.0016450909759502216 60-61 0.001572513432893594 62-63 0.0 64-65 0.001040278117144993 66-67 0.0 68-69 0.0 70-71 1.6934760046546398E-4 72-73 0.0012822032606670845 74-75 0.0 76-77 0.19491908813574904 78-79 0.10523743743210975 80-81 0.0014999358898369667 82-83 0.0 84-85 7.01582916214065E-4 86-87 0.12297055045227905 88-89 0.5865233179549584 90-91 0.31038995913884326 92-93 0.29367293172146675 94-95 0.0026127915500385873 96-97 0.0026853690930952146 98-99 0.003145026865787188 100-101 6.531978875096468E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2066755.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.78867544521082 #Duplication Level Percentage of deduplicated Percentage of total 1 73.385065432321 40.20670532496995 2 14.603477025810847 16.00210326277486 3 5.04954689920557 8.29973958617834 4 2.390414096871424 5.2387048853258085 5 1.272957238559802 3.4871820499542396 6 0.7792963970623856 2.5617970424563907 7 0.5140413790058244 1.97145523958311 8 0.35950816307251926 1.5757580853187347 9 0.271015652366568 1.3363729756275577 >10 1.26814251493988 12.40357617404279 >50 0.07367368280224595 2.772205206571484 >100 0.031272466841381734 2.9954718786943006 >500 0.0010593674271083642 0.40570895360606507 >1k 4.414030946284785E-4 0.4924807541269563 >5k 8.828061892569569E-5 0.25073858076941086 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5184 0.2508279888037044 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3837 0.18565335513885292 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2477 0.11984971610084408 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.8385028704418276E-5 0.0 0.0 0.0 0.0 3 4.8385028704418276E-5 0.0 0.0 0.0 0.0 4 4.8385028704418276E-5 0.0 0.0 0.0 0.0 5 4.8385028704418276E-5 0.0 0.0 0.0 0.0 6 4.8385028704418276E-5 0.0 0.0 0.0 0.0 7 4.8385028704418276E-5 0.0 0.0 0.0 0.0 8 4.8385028704418276E-5 0.0 0.0 0.0 0.0 9 4.8385028704418276E-5 0.0 0.0 0.0 0.0 10-11 4.8385028704418276E-5 0.0 0.0 0.0 0.0 12-13 4.8385028704418276E-5 0.0 0.0 0.0 0.0 14-15 4.8385028704418276E-5 0.0 0.0 2.4192514352209138E-5 4.8385028704418276E-5 16-17 4.8385028704418276E-5 0.0 0.0 9.677005740883655E-5 4.8385028704418276E-5 18-19 7.257754305662741E-5 0.0 0.0 2.1773262916988226E-4 4.8385028704418276E-5 20-21 9.677005740883655E-5 0.0 0.0 4.5965777269197364E-4 4.8385028704418276E-5 22-23 9.677005740883655E-5 0.0 0.0 8.951230310317382E-4 4.8385028704418276E-5 24-25 2.4192514352209139E-4 0.0 0.0 0.0011612406889060386 4.8385028704418276E-5 26-27 2.4192514352209139E-4 0.0 0.0 0.00140316583242813 4.8385028704418276E-5 28-29 2.6611765787430057E-4 0.0 0.0 0.0020563637199377768 4.8385028704418276E-5 30-31 3.3869520093092795E-4 0.0 0.0 0.0030482568083783513 4.8385028704418276E-5 32-33 3.3869520093092795E-4 0.0 0.0 0.004911080413498455 4.8385028704418276E-5 34-35 3.628877152831371E-4 0.0 0.0 0.0073061393343671595 4.8385028704418276E-5 36-37 3.870802296353462E-4 0.0 0.0 0.012483337405739916 4.8385028704418276E-5 38-39 3.870802296353462E-4 2.4192514352209138E-5 0.0 0.022208728175327992 4.8385028704418276E-5 40-41 3.870802296353462E-4 4.8385028704418276E-5 0.0 0.035030760781998835 4.8385028704418276E-5 42-43 3.870802296353462E-4 7.257754305662741E-5 0.0 0.045312579381687715 4.8385028704418276E-5 44-45 3.870802296353462E-4 9.677005740883655E-5 0.0 0.05857007724669833 4.8385028704418276E-5 46-47 4.1127274398755533E-4 9.677005740883655E-5 0.0 0.07531129717842705 4.8385028704418276E-5 48-49 4.354652583397645E-4 9.677005740883655E-5 0.0 0.09539108409076064 4.8385028704418276E-5 50-51 4.354652583397645E-4 9.677005740883655E-5 0.0 0.11943844335685652 4.8385028704418276E-5 52-53 4.354652583397645E-4 9.677005740883655E-5 0.0 0.14096978113032266 4.8385028704418276E-5 54-55 4.354652583397645E-4 9.677005740883655E-5 0.0 0.16397686227927355 4.8385028704418276E-5 56-57 4.354652583397645E-4 9.677005740883655E-5 0.0 0.19160471366949638 4.8385028704418276E-5 58-59 4.354652583397645E-4 9.677005740883655E-5 0.0 0.22535327119082812 4.8385028704418276E-5 60-61 4.354652583397645E-4 9.677005740883655E-5 0.0 0.2617630052909029 4.8385028704418276E-5 62-63 4.354652583397645E-4 9.677005740883655E-5 0.0 0.2996968677951668 4.8385028704418276E-5 64-65 4.354652583397645E-4 9.677005740883655E-5 0.0 0.3395903239619597 4.8385028704418276E-5 66-67 4.354652583397645E-4 9.677005740883655E-5 0.0 0.38715280717840284 4.8385028704418276E-5 68-69 4.354652583397645E-4 9.677005740883655E-5 0.0 0.44250528001625733 4.8385028704418276E-5 70-71 4.354652583397645E-4 9.677005740883655E-5 0.0 0.5061315927625674 4.8385028704418276E-5 72-73 4.354652583397645E-4 9.677005740883655E-5 0.0 0.5706046435112048 4.8385028704418276E-5 74-75 4.354652583397645E-4 9.677005740883655E-5 0.0 0.6379082184390505 4.8385028704418276E-5 76-77 4.354652583397645E-4 9.677005740883655E-5 0.0 0.7096632160077029 4.8385028704418276E-5 78-79 4.354652583397645E-4 9.677005740883655E-5 0.0 0.7977965457928008 4.8385028704418276E-5 80-81 5.32235315748601E-4 9.677005740883655E-5 0.0 0.9010743895623816 4.8385028704418276E-5 82-83 5.32235315748601E-4 9.677005740883655E-5 0.0 1.0070376024250578 4.8385028704418276E-5 84-85 5.564278301008102E-4 9.677005740883655E-5 0.0 1.112807275172916 4.8385028704418276E-5 86-87 5.806203444530194E-4 9.677005740883655E-5 0.0 1.2237057609634427 4.8385028704418276E-5 88-89 5.806203444530194E-4 9.677005740883655E-5 0.0 1.3515631993148678 4.8385028704418276E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7800 0.0 31.591043 1 GGTATCA 5165 0.0 31.037619 1 GTACATG 8210 0.0 27.579895 1 TCAACGC 9200 0.0 26.504232 4 ATCAACG 9195 0.0 26.467052 3 TACATGG 8175 0.0 26.409376 2 CAACGCA 9305 0.0 26.256134 5 TATCAAC 9475 0.0 25.840744 2 ACATGGG 8290 0.0 25.579552 3 GTACCTG 1935 0.0 25.567083 1 AACGCAG 9515 0.0 25.527079 6 TACCTGG 1960 0.0 23.482817 2 CTCGCGC 370 0.0 23.079504 60-61 ATTCGCG 405 0.0 22.87194 80-81 ACGCAGA 10900 0.0 22.152933 7 CGCGATC 420 0.0 22.027073 22-23 TCGCGCT 410 0.0 21.98495 60-61 CATGGGG 4950 0.0 21.850912 4 CGCGGAA 385 0.0 21.713375 82-83 CGCAGAG 11130 0.0 21.524652 8 >>END_MODULE