FastQCFastQC Report
Thu 26 May 2016
SRR936350_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936350_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029992
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61410.30251350744239386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43250.21305502681783967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28550.14064094833871268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46550.037.7380181
GTATCAA74200.036.0553251
TCAACGC89550.029.232554
ATCAACG90100.029.107363
CAACGCA90300.029.09465
TATCAAC92250.028.5843372
GTACCTG19550.028.5798631
TACATGG96150.028.2126962
TACCTGG18650.028.1762642
AACGCAG93600.028.1194046
GTACATG99400.028.105451
ACATGGG99750.026.3389193
ACGCAGA109200.024.0595917
CGCAGAG111300.023.4791958
CATGGGG56650.023.3972744
CTTAGCG2457.827111E-921.2541873
CGATACT2257.4220225E-821.0389794
GTGTACG2551.2372766E-820.4257221
AGTACAT69600.020.405692
GCAGAGT125750.020.253639
Produced by FastQC (version 0.11.5)