##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936350_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2029992 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.602903361195512 33.0 31.0 34.0 30.0 34.0 2 31.689682028303558 33.0 31.0 34.0 30.0 34.0 3 31.75494041355828 34.0 31.0 34.0 30.0 34.0 4 35.255299035661224 37.0 35.0 37.0 33.0 37.0 5 35.2340270306484 37.0 35.0 37.0 33.0 37.0 6 35.32790079960906 37.0 35.0 37.0 33.0 37.0 7 35.26850105813225 37.0 35.0 37.0 33.0 37.0 8 35.267485783195205 37.0 35.0 37.0 33.0 37.0 9 36.91234004863073 39.0 37.0 39.0 33.0 39.0 10-11 36.82910326740204 39.0 37.0 39.0 33.0 39.0 12-13 36.727750404927704 39.0 37.0 39.0 33.0 39.0 14-15 37.935117231989096 40.0 38.0 41.0 33.0 41.0 16-17 37.87701355473322 40.0 38.0 41.0 33.0 41.0 18-19 37.83578186515021 40.0 38.0 41.0 33.0 41.0 20-21 37.75750249262066 40.0 38.0 41.0 32.0 41.0 22-23 37.58992991105384 40.0 38.0 41.0 32.0 41.0 24-25 37.74499530047409 40.0 38.0 41.0 32.0 41.0 26-27 37.689079809181514 40.0 38.0 41.0 32.0 41.0 28-29 37.550786899652806 40.0 38.0 41.0 32.0 41.0 30-31 37.382895597618116 40.0 37.0 41.0 32.0 41.0 32-33 37.18434456884559 40.0 37.0 41.0 31.0 41.0 34-35 36.91120531509484 40.0 36.5 41.0 30.5 41.0 36-37 36.70108798458319 40.0 36.0 41.0 30.0 41.0 38-39 36.525996407867616 40.0 36.0 41.0 30.0 41.0 40-41 36.325645864614245 40.0 36.0 41.0 30.0 41.0 42-43 36.12149161178961 39.5 35.0 41.0 29.0 41.0 44-45 35.905877215279666 39.0 35.0 41.0 28.5 41.0 46-47 35.5901141482331 39.0 35.0 41.0 27.0 41.0 48-49 35.382007416777995 39.0 35.0 41.0 26.5 41.0 50-51 35.0615509814817 38.5 34.5 40.5 26.0 41.0 52-53 35.067934750481776 39.0 35.0 40.0 26.0 41.0 54-55 35.135014571485996 39.0 35.0 41.0 26.0 41.0 56-57 34.95614219169336 39.0 35.0 41.0 26.0 41.0 58-59 34.701702272718315 38.0 34.5 40.5 26.0 41.0 60-61 34.391678883463584 38.0 34.0 40.0 25.0 41.0 62-63 34.069012833548115 37.0 34.0 40.0 24.0 41.0 64-65 33.7227410255804 37.0 34.0 40.0 23.0 41.0 66-67 33.34757501507395 36.0 33.0 39.0 22.5 41.0 68-69 32.95504834501811 35.5 33.0 39.0 21.5 41.0 70-71 32.553318436722904 35.0 33.0 38.5 20.5 40.0 72-73 32.14724269849339 35.0 32.5 37.5 19.5 40.0 74-75 31.737842070313576 35.0 32.0 37.0 18.0 39.0 76-77 31.353026514390205 35.0 32.0 36.5 16.0 39.0 78-79 30.972940287449408 35.0 31.5 36.0 11.5 38.5 80-81 30.596479936866746 35.0 31.0 35.5 7.5 37.0 82-83 30.26172615458583 35.0 31.0 35.0 6.0 37.0 84-85 29.95726559513535 34.0 31.0 35.0 2.0 36.5 86-87 29.647426689366263 34.0 30.0 35.0 2.0 36.0 88-89 29.32465103310752 34.0 30.0 35.0 2.0 36.0 90-91 28.9742282235595 34.0 29.5 35.0 2.0 35.0 92-93 28.66453094396431 34.0 29.0 35.0 2.0 35.0 94-95 28.278268584309693 34.0 29.0 35.0 2.0 35.0 96-97 27.841425729756573 34.0 29.0 35.0 2.0 35.0 98-99 27.322346590528436 34.0 28.0 35.0 2.0 35.0 100-101 24.047139348332408 29.5 14.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4238260748649729 1101 2 0.30712698798040705 1101 3 0.36361359983220964 1101 4 0.18525152642290976 1101 5 0.4352429906814521 1101 6 0.3465126814103172 1101 7 0.45980540965048533 1101 8 0.4005426054093988 1101 9 0.4501039003340921 1101 10-11 0.7078480325511336 1101 12-13 0.6224060670332108 1101 14-15 0.7889803961138355 1101 16-17 0.7982693969351615 1101 18-19 0.8722370238575934 1101 20-21 1.00865288041863 1101 22-23 0.9768984709260096 1101 24-25 0.8529608348693287 1101 26-27 0.7573833664881704 1101 28-29 0.7580243579578863 1101 30-31 0.8741925639477302 1101 32-33 0.8919376407217996 1101 34-35 0.6995675688405498 1101 36-37 0.710568142326359 1101 38-39 0.7417306666247256 1101 40-41 0.6531545402843832 1101 42-43 0.853250248760645 1101 44-45 0.8753111706671959 1101 46-47 0.8435186196311832 1101 48-49 0.7223364863747008 1101 50-51 0.8475522310650518 1101 52-53 0.936368018995303 1101 54-55 0.9713732105016888 1101 56-57 0.918145859444671 1101 58-59 1.0547752052590127 1101 60-61 1.059984072194112 1101 62-63 1.0400096082691093 1101 64-65 0.9852087859874032 1101 66-67 1.0484425031654396 1101 68-69 1.0202244049851998 1101 70-71 1.1140681199644504 1101 72-73 1.0153822667603194 1101 74-75 0.861510055896499 1101 76-77 0.8399778405017351 1101 78-79 0.9649255886459862 1101 80-81 0.9462177112222889 1101 82-83 1.1311036586105203 1101 84-85 1.071123524873311 1101 86-87 1.1430903699423318 1101 88-89 1.08937967188848 1101 90-91 1.1306722717198063 1101 92-93 1.2575945117917655 1101 94-95 1.2157148844165349 1101 96-97 1.3103828918484304 1101 98-99 1.477555686791657 1101 100-101 1.7283527520950344 1102 1 0.34715117930003103 1102 2 0.3566160061591397 1102 3 0.29415488918044375 1102 4 0.34216396156653417 1102 5 0.31827251000715506 1102 6 0.37237011526597286 1102 7 0.2920213215518146 1102 8 0.25653142541365526 1102 9 0.2805876323610761 1102 10-11 0.40089551284450664 1102 12-13 0.4958430742066753 1102 14-15 0.556848860932881 1102 16-17 0.5535642208232403 1102 18-19 0.5578102427443596 1102 20-21 0.5189921602711891 1102 22-23 0.5579296720046969 1102 24-25 0.6180797491943579 1102 26-27 0.595928174798523 1102 28-29 0.4766646112101043 1102 30-31 0.5630811102335187 1102 32-33 0.3520329443398822 1102 34-35 0.33440876266675446 1102 36-37 0.3739427678986331 1102 38-39 0.30962068332019754 1102 40-41 0.32010433629635315 1102 42-43 0.33708505667914324 1102 44-45 0.17330809880212428 1102 46-47 0.4254167136230649 1102 48-49 0.5929123293871044 1102 50-51 0.48941625317836923 1102 52-53 0.4352693872022044 1102 54-55 0.544885344111762 1102 56-57 0.5673686750126379 1102 58-59 0.6186484147027755 1102 60-61 0.7351442980295104 1102 62-63 0.8146988726081759 1102 64-65 0.5282327230238053 1102 66-67 0.7647629586905182 1102 68-69 0.6884793582230841 1102 70-71 0.7241641623204771 1102 72-73 0.7110294622220295 1102 74-75 0.7992816625358934 1102 76-77 0.6844386549506716 1102 78-79 0.726038295002791 1102 80-81 0.6875690170861333 1102 82-83 0.725969494164886 1102 84-85 0.6941524596324733 1102 86-87 0.8194489571927406 1102 88-89 0.7272806861435512 1102 90-91 0.7275381599494004 1102 92-93 0.6648645924555261 1102 94-95 0.5833157012679848 1102 96-97 0.877157735444758 1102 98-99 0.9187181005761254 1102 100-101 0.7746804536757388 1103 1 0.05791431233040001 1103 2 0.11454314497320439 1103 3 0.15675699565145962 1103 4 0.011572466683645644 1103 5 -0.0032980075410833365 1103 6 -0.06051371941197914 1103 7 0.13916234036323516 1103 8 0.09865383072784084 1103 9 0.12284595519525254 1103 10-11 0.12057549285147218 1103 12-13 0.22040778582195486 1103 14-15 0.31866499459304265 1103 16-17 0.1804410335135529 1103 18-19 0.06020398828358253 1103 20-21 0.1645037660302009 1103 22-23 0.20919428431250253 1103 24-25 0.26347970841910495 1103 26-27 0.26305035306155844 1103 28-29 0.14040137843380762 1103 30-31 0.18130774361605262 1103 32-33 0.3060890644959784 1103 34-35 0.07527339852524051 1103 36-37 0.22369330914161623 1103 38-39 0.27485899464836194 1103 40-41 0.41167939173598 1103 42-43 0.24222652834018277 1103 44-45 0.30296871526010705 1103 46-47 0.3858996342087124 1103 48-49 0.38934624826188724 1103 50-51 0.4105606035349041 1103 52-53 0.42083598005375933 1103 54-55 0.3522789440250733 1103 56-57 0.35849366808366057 1103 58-59 0.44834470168040497 1103 60-61 0.39290758420197136 1103 62-63 0.4672189995188134 1103 64-65 0.39027268214832844 1103 66-67 0.43993355240267107 1103 68-69 0.38154862949534873 1103 70-71 0.3219525901023914 1103 72-73 0.2865159858746509 1103 74-75 0.2747831559061602 1103 76-77 0.2957696664119034 1103 78-79 0.31325005355941116 1103 80-81 0.372088794494573 1103 82-83 0.39262645243726624 1103 84-85 0.41771642370139617 1103 86-87 0.4590124489952565 1103 88-89 0.335658153524129 1103 90-91 0.4222899585860276 1103 92-93 0.36796138232798015 1103 94-95 0.4663419205092083 1103 96-97 0.3741919775777518 1103 98-99 0.47936628308641716 1103 100-101 0.7544880896723392 1104 1 0.2508735438919629 1104 2 0.18147158536254437 1104 3 0.19130990807459014 1104 4 0.2379268942859909 1104 5 0.1744402553053348 1104 6 0.1943655111744107 1104 7 0.17717529801013399 1104 8 0.1504700954392817 1104 9 0.13204704251614174 1104 10-11 0.27114048526338763 1104 12-13 0.38859923109326644 1104 14-15 0.4657975209685006 1104 16-17 0.4423895259528976 1104 18-19 0.19395344736582842 1104 20-21 0.3971259829702305 1104 22-23 0.513094072821275 1104 24-25 0.39339139407122303 1104 26-27 0.17046016454790447 1104 28-29 0.3756971004599876 1104 30-31 0.38821792993554993 1104 32-33 0.39277806878065746 1104 34-35 0.36084536953813995 1104 36-37 0.36144982605787135 1104 38-39 0.19538491908443945 1104 40-41 0.26306246512404385 1104 42-43 0.3513284220094661 1104 44-45 0.3925410397741942 1104 46-47 0.4239106110300028 1104 48-49 0.330344462471416 1104 50-51 0.2825882985243311 1104 52-53 0.332304960324727 1104 54-55 0.4618118958209223 1104 56-57 0.37133536050106386 1104 58-59 0.36808484783485795 1104 60-61 0.30955901374857575 1104 62-63 0.3575679600424806 1104 64-65 0.4755137029134815 1104 66-67 0.6017357684894122 1104 68-69 0.38375907850281266 1104 70-71 0.4257234725754415 1104 72-73 0.47789086169212425 1104 74-75 0.47776947906718803 1104 76-77 0.45982088141985855 1104 78-79 0.4862357062871574 1104 80-81 0.4567357199484867 1104 82-83 0.3728812780331907 1104 84-85 0.1710103843164852 1104 86-87 0.383666261627436 1104 88-89 0.5614552072745944 1104 90-91 0.6957427814148893 1104 92-93 0.4694317644140007 1104 94-95 0.4162349559263703 1104 96-97 0.7181602112298435 1104 98-99 0.9428352877367878 1104 100-101 1.1016990872161152 1105 1 0.3062353619766327 1105 2 0.23876434662613377 1105 3 0.19754710685635857 1105 4 0.18811687178553171 1105 5 0.25885646038604904 1105 6 0.31795418614071735 1105 7 0.39412188464878284 1105 8 0.26240216660959703 1105 9 0.24951220836056365 1105 10-11 0.356707241575684 1105 12-13 0.23894163585220696 1105 14-15 0.20535573423011755 1105 16-17 0.3692839578311222 1105 18-19 0.31499004178167667 1105 20-21 0.30904811000581134 1105 22-23 0.35605243806488573 1105 24-25 0.31453588977000635 1105 26-27 0.31921462456654837 1105 28-29 0.2805778895790212 1105 30-31 0.4066286790999456 1105 32-33 0.4097898117648455 1105 34-35 0.256719592559854 1105 36-37 0.29425641708974837 1105 38-39 0.19741738148966448 1105 40-41 0.2077459151154386 1105 42-43 0.24115052589154118 1105 44-45 0.36479660700545224 1105 46-47 0.3304014594576401 1105 48-49 0.4357950356691518 1105 50-51 0.35330504800931095 1105 52-53 0.3921529189571302 1105 54-55 0.6098916388763556 1105 56-57 0.6126122764538309 1105 58-59 0.651525785611291 1105 60-61 0.43428963159102096 1105 62-63 0.3730373232022828 1105 64-65 0.3856855138146287 1105 66-67 0.39784315920783087 1105 68-69 0.5000729510160298 1105 70-71 0.6091081592016181 1105 72-73 0.6684878659844244 1105 74-75 0.755966147336796 1105 76-77 0.7444465070098296 1105 78-79 0.7017849193647372 1105 80-81 0.6751286587223042 1105 82-83 0.6341036051388258 1105 84-85 0.4957270506863125 1105 86-87 0.4599581086243063 1105 88-89 0.581014578211601 1105 90-91 0.7278589229718726 1105 92-93 0.685641891402085 1105 94-95 0.6354446495282815 1105 96-97 0.6859809912062182 1105 98-99 0.8933921592823175 1105 100-101 0.8658381626193723 1106 1 0.16317231523132492 1106 2 0.11851590635520637 1106 3 0.3280278027260657 1106 4 0.19194133003474434 1106 5 0.22128142151919405 1106 6 0.17179546129808898 1106 7 0.14484489778305942 1106 8 0.11076975887547036 1106 9 0.24148907649691864 1106 10-11 0.26536010328374715 1106 12-13 0.2439183584603697 1106 14-15 0.18115476520028295 1106 16-17 0.19562439071912507 1106 18-19 0.18651356252741635 1106 20-21 0.2346457510250488 1106 22-23 0.18039264141135192 1106 24-25 0.2730394213207248 1106 26-27 0.21213933408685648 1106 28-29 0.35201699827435107 1106 30-31 0.2212995162459208 1106 32-33 0.18457381915574445 1106 34-35 0.24833776644013028 1106 36-37 0.18426216132407802 1106 38-39 0.13808189001019144 1106 40-41 0.024058572337864348 1106 42-43 0.17284924436054894 1106 44-45 0.1890902470245095 1106 46-47 0.2750409382673382 1106 48-49 0.19177343939854552 1106 50-51 0.3023401334053162 1106 52-53 0.2294847178365913 1106 54-55 0.17930915870563524 1106 56-57 0.29027315702188616 1106 58-59 0.29163113549554254 1106 60-61 0.20655224192261556 1106 62-63 0.26193822077805606 1106 64-65 0.1366689683122857 1106 66-67 0.12572607707382133 1106 68-69 0.27541087688891963 1106 70-71 0.27477663343672276 1106 72-73 0.36065514537892085 1106 74-75 0.32727781206260786 1106 76-77 0.2667883604353065 1106 78-79 0.24769408185992958 1106 80-81 0.4619798300818978 1106 82-83 0.41923179098887076 1106 84-85 0.4536874858127753 1106 86-87 0.3490640557152638 1106 88-89 0.25255045888645 1106 90-91 0.2505732830166103 1106 92-93 0.2039579393460791 1106 94-95 0.25614006926321764 1106 96-97 0.2850755836802712 1106 98-99 0.3075711054246355 1106 100-101 0.2795852232203302 1107 1 -0.07039046816959527 1107 2 0.04780984832657609 1107 3 0.13359952030285172 1107 4 -0.03362456039429418 1107 5 0.10972858660593943 1107 6 0.014227857417125733 1107 7 0.0011801275364362596 1107 8 -0.042485108865619736 1107 9 0.10819880486422306 1107 10-11 0.04806202997634301 1107 12-13 0.028793852422857924 1107 14-15 0.21970787441873085 1107 16-17 0.008400662056054387 1107 18-19 0.21020759199419814 1107 20-21 0.25333863534851986 1107 22-23 0.27749991238049176 1107 24-25 0.12579089640937724 1107 26-27 0.1492791982749111 1107 28-29 0.19283134658196133 1107 30-31 0.3224853496324229 1107 32-33 0.33783868301305375 1107 34-35 0.2659853216106143 1107 36-37 0.417989145634003 1107 38-39 0.34770529809193107 1107 40-41 0.3181072669479832 1107 42-43 -0.08271866587197962 1107 44-45 0.23655959790318093 1107 46-47 0.30825127037066125 1107 48-49 0.25842313226787894 1107 50-51 0.42552389053967943 1107 52-53 0.2928140831037922 1107 54-55 0.38795691644447317 1107 56-57 0.3357346115567523 1107 58-59 0.47410597394801357 1107 60-61 0.5688350138221168 1107 62-63 0.613741387841678 1107 64-65 0.3938852087082978 1107 66-67 0.4175327016176382 1107 68-69 0.4339179686923771 1107 70-71 0.5354419749319774 1107 72-73 0.36426859438887504 1107 74-75 0.3588404020092817 1107 76-77 0.40932459856161074 1107 78-79 0.4721576064728623 1107 80-81 0.49107852438331534 1107 82-83 0.6723863741640557 1107 84-85 0.5190598808322413 1107 86-87 0.2878665092325896 1107 88-89 0.6402739345595805 1107 90-91 0.8239552131549281 1107 92-93 0.38952363573286775 1107 94-95 0.3852732795659186 1107 96-97 0.5610388050286836 1107 98-99 0.2746277284249352 1107 100-101 0.4000298975101124 1108 1 0.08786105437344816 1108 2 0.06094773170616108 1108 3 0.14011722525540193 1108 4 0.008070104489441121 1108 5 0.11506980647705234 1108 6 0.10117296075294036 1108 7 0.0920237026175812 1108 8 0.07635164360998203 1108 9 0.14847759991055653 1108 10-11 0.13729962732614354 1108 12-13 0.051585241067229504 1108 14-15 0.05233929245003566 1108 16-17 0.17994027841329086 1108 18-19 0.10100362610275226 1108 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-2.1050202822083506 2316 68-69 -2.1046695940516322 2316 70-71 -2.0991787724188313 2316 72-73 -2.001650953854295 2316 74-75 -1.9559602114876533 2316 76-77 -2.237252367417195 2316 78-79 -2.2673327727797847 2316 80-81 -2.049042780691977 2316 82-83 -2.1388413168565847 2316 84-85 -2.2011715748286633 2316 86-87 -2.325839569964284 2316 88-89 -2.376353226622797 2316 90-91 -2.3485907991150086 2316 92-93 -2.432544824947559 2316 94-95 -2.2724756049851145 2316 96-97 -2.5765622825340593 2316 98-99 -2.298434178992803 2316 100-101 -2.2525077488925653 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19334.0 3 4232.0 4 3612.0 5 2931.0 6 2947.0 7 3144.0 8 3759.0 9 4433.0 10 5266.0 11 6237.0 12 7958.0 13 10939.0 14 11363.0 15 10442.0 16 12351.0 17 15800.0 18 14438.0 19 10492.0 20 10087.0 21 10440.0 22 11137.0 23 12128.0 24 13475.0 25 15807.0 26 18629.0 27 22351.0 28 26556.0 29 32463.0 30 39408.0 31 48470.0 32 61817.0 33 80354.0 34 112680.0 35 169119.0 36 281506.0 37 447511.0 38 396762.0 39 69582.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.9934925981014 17.523904993115085 12.831764637465168 23.650837771318344 2 15.931844820051621 18.73567845038801 40.19365223102091 25.138824498539453 3 20.401525303484156 23.33211808292606 27.68322363235062 28.583132981239164 4 12.86679235671802 14.572229329988605 35.38976314141708 37.1712151718763 5 14.378819640310276 37.275895960449404 33.12303398602329 15.222250413217035 6 34.87532877899401 33.96024058813937 16.443900783256375 14.720529849610244 7 31.1363790872985 31.412650759697836 19.05446906870632 18.396501084297345 8 27.960977647672614 34.76747342486566 18.28199768352817 18.989551243933548 9 27.497742258273828 15.86361022878942 18.978825757519772 37.65982175541698 10-11 26.553784757518518 25.72070533171325 25.75026287742215 21.975247033346086 12-13 26.981642580621006 22.524295425277096 26.89316121836274 23.60090077573916 14-15 23.729459021994376 24.01505162171223 24.828254010403413 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23.63139126511116 25.44271329009889 27.585954259539825 23.339941185250126 42-43 23.635884849275328 25.377569630390994 27.297375358030035 23.68917016230365 44-45 23.53244326818615 25.69062476097971 27.21666643151402 23.56026553932012 46-47 23.84754503030405 25.86525713142779 26.905649989949808 23.381547848318352 48-49 24.010647104724256 25.80137980614981 26.669006943591732 23.518966145534197 50-51 23.80549873408194 26.09026666726088 26.56902781553497 23.535206783122213 52-53 24.004221390806173 25.99895975345018 26.484895011099347 23.511923844644304 54-55 23.891259932682267 25.989753328386072 26.50424040321331 23.614746335718348 56-57 23.675421122769638 26.326360988398456 26.448683118852955 23.54953476997895 58-59 23.84594098635272 25.924888211477697 26.53525131564683 23.69391948652276 60-61 23.655077526449976 25.99412478532251 26.723143440329494 23.627654247898022 62-63 23.63848175126118 26.06037761439198 26.795118687895545 23.50602194645129 64-65 23.663431112853768 25.98272737556757 26.744868499775855 23.608973011802803 66-67 23.578323053626143 26.228881394790005 26.497218750007733 23.69557680157612 68-69 23.58658588659022 26.131504848661123 26.711735183271344 23.57017408147731 70-71 23.678242677720714 25.93736287316134 26.539638179428295 23.84475626968965 72-73 23.68490551794039 26.293375179601924 26.297978929182698 23.72374037327499 74-75 23.609942472012694 26.437252806528566 26.234064321730944 23.718740399727796 76-77 23.847710717886176 26.288212352353984 26.242521013436864 23.62155591632297 78-79 23.732613557186685 26.342618891153656 26.33885664994697 23.585910901712687 80-81 23.63224489482729 26.416058735630504 26.239626105395725 23.71207026414648 82-83 23.751931291803402 26.50477396027071 26.215494789410602 23.52779995851528 84-85 23.745159457884036 26.502965895603943 26.02156632455425 23.73030832195778 86-87 23.722444170515697 26.252704221308026 26.30240743766058 23.722444170515697 88-89 23.829199942566582 26.335277487584975 26.097671470939186 23.73785109890926 90-91 23.677288341744454 26.446358960238488 26.17011798678867 23.706234711228394 92-93 23.55355275801018 26.43697711596658 26.23422976432137 23.77524036170187 94-95 23.761872867314473 26.300798941806804 26.18785602513209 23.74947216574664 96-97 23.84888306878071 26.12624784653523 26.13273113030361 23.89213795438045 98-99 23.55632820922419 26.21134370724806 26.057909930587357 24.174418152940394 100-101 23.689715687283726 26.346214376776704 25.890877525002576 24.07319241093699 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 148.0 1 121.5 2 183.0 3 312.0 4 290.5 5 285.0 6 587.0 7 1163.5 8 1841.0 9 2116.5 10 1864.5 11 1671.0 12 1742.0 13 2000.0 14 2290.5 15 2488.5 16 2671.0 17 2843.5 18 2947.0 19 3037.5 20 3176.5 21 3454.5 22 3873.5 23 4449.5 24 5284.5 25 6071.5 26 7549.5 27 9688.0 28 11513.5 29 13524.5 30 15792.5 31 19043.5 32 23236.0 33 26549.5 34 28840.5 35 31271.5 36 34959.5 37 38687.5 38 42217.5 39 45778.5 40 48246.5 41 51175.5 42 53611.5 43 56098.0 44 59951.0 45 64085.5 46 67815.0 47 71467.0 48 76823.0 49 79801.0 50 78424.0 51 78313.0 52 80306.0 53 82126.0 54 83877.0 55 80871.0 56 73876.0 57 67360.0 58 63604.5 59 59329.5 60 52079.5 61 44519.0 62 36716.0 63 28795.0 64 21530.0 65 16227.5 66 11664.0 67 8675.0 68 6686.5 69 5168.0 70 4131.0 71 2937.0 72 2200.0 73 1825.5 74 1392.5 75 939.0 76 637.0 77 390.0 78 229.0 79 124.0 80 77.5 81 52.0 82 42.0 83 28.0 84 28.0 85 28.0 86 20.5 87 18.0 88 15.5 89 14.5 90 19.0 91 18.5 92 10.5 93 12.5 94 16.0 95 17.0 96 17.0 97 12.5 98 11.0 99 9.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04522185309104666 2 0.013349806304655388 3 0.011428616467454059 4 0.006453227401881386 5 0.009211858962990987 6 0.006551749957635302 7 0.005221695454957458 8 0.008916291295729245 9 0.01522173486397976 10-11 0.002635478366417208 12-13 0.009162597685114031 14-15 0.008743876823159894 16-17 0.018916330704751544 18-19 0.012413842024993203 20-21 0.0033005056177561293 22-23 0.010394129632037959 24-25 0.021748854182676584 26-27 0.008029588293944016 28-29 0.018275934092351103 30-31 0.015147842947164323 32-33 0.013177391832086037 34-35 0.26546902647892207 36-37 0.4713072760877875 38-39 0.469016626666509 40-41 0.4886965071783534 42-43 0.47958317077111634 44-45 0.49396746391118784 46-47 0.49901674489357595 48-49 0.5068000267981352 50-51 0.5176867692089427 52-53 0.5063320446583041 54-55 0.4935487430492337 56-57 0.4884748314279071 58-59 0.4868492092579675 60-61 0.48327776661188815 62-63 0.48061765760653247 64-65 0.49707092441743617 66-67 0.4722925016453267 68-69 0.49776058230771353 70-71 0.47963243204899325 72-73 0.4876620203429373 74-75 0.5020955747608857 76-77 0.4884502007889686 78-79 0.4886472459004765 80-81 0.4905191744598008 82-83 0.4911842017111398 84-85 0.4898787778474004 86-87 0.49271130132532537 88-89 0.5055192335733343 90-91 0.5290907550374583 92-93 0.5164306066230803 94-95 0.4899034084863389 96-97 0.46359788610004377 98-99 0.4556914510007921 100-101 0.46305601204339725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2029992.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.25097419148051 #Duplication Level Percentage of deduplicated Percentage of total 1 70.22884958695629 33.183815593306385 2 14.537289847737247 13.738022148190087 3 5.791437387593276 8.209531755982354 4 2.9051647294869447 5.490874546199484 5 1.6702228522750515 3.9459828443434706 6 1.0878792610958647 3.0842012933692557 7 0.752991207098455 2.4905697665114594 8 0.5170871076621263 1.9546295663112345 9 0.3837713816937411 1.6320214486655802 >10 1.9465494429731913 16.34800946605748 >50 0.12220218343310058 3.94427445915389 >100 0.05384307057093639 4.483341434415309 >500 0.002086108787535981 0.6683584447539169 >1k 5.21527196883994E-4 0.5237059828888068 >5k 1.0430543937679879E-4 0.30266124985132337 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6141 0.30251350744239386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4325 0.21305502681783967 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2855 0.14064094833871268 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4778383363087146E-4 2 0.0 0.0 0.0 0.0 1.4778383363087146E-4 3 0.0 0.0 0.0 0.0 1.4778383363087146E-4 4 0.0 0.0 0.0 0.0 1.4778383363087146E-4 5 0.0 0.0 0.0 0.0 1.4778383363087146E-4 6 0.0 0.0 0.0 0.0 1.4778383363087146E-4 7 0.0 0.0 0.0 0.0 1.4778383363087146E-4 8 0.0 0.0 0.0 0.0 1.4778383363087146E-4 9 0.0 0.0 0.0 0.0 1.4778383363087146E-4 10-11 0.0 0.0 0.0 0.0 1.4778383363087146E-4 12-13 0.0 0.0 0.0 4.926127787695715E-5 7.881804460313144E-4 14-15 0.0 0.0 0.0 4.926127787695715E-5 8.12811084969793E-4 16-17 0.0 0.0 0.0 1.2315319469239288E-4 9.113336407237072E-4 18-19 0.0 0.0 0.0 1.970451115078286E-4 9.85225557539143E-4 20-21 0.0 0.0 0.0 4.679821398310929E-4 0.001157640030108493 22-23 0.0 0.0 0.0 0.0012315319469239287 0.0012315319469239287 24-25 0.0 0.0 0.0 0.0018965591982628505 0.0012315319469239287 26-27 0.0 0.0 0.0 0.0020689736708322 0.0012561625858624073 28-29 0.0 0.0 0.0 0.0025862170885402502 0.001280793224800886 30-31 0.0 0.0 0.0 0.00431036181423375 0.001280793224800886 32-33 0.0 0.0 0.0 0.0066010112355122585 0.001280793224800886 34-35 0.0 0.0 0.0 0.010000039409022301 0.0014285770584317574 36-37 0.0 0.0 0.0 0.016896618311796303 0.0014285770584317574 38-39 0.0 0.0 0.0 0.029507505448297333 0.001453207697370236 40-41 0.0 0.0 0.0 0.04214302322373684 0.0014778383363087146 42-43 0.0 0.0 0.0 0.057684956393916825 0.0015024689752471932 44-45 0.0 0.0 0.0 0.0742860070384514 0.0015270996141856716 46-47 0.0 0.0 0.0 0.09603486122112796 0.0016009915310011072 48-49 0.0 0.0 0.0 0.12120737421625308 0.001674883447816543 50-51 0.0 0.0 0.0 0.1493601945229341 0.0018226672814474147 52-53 0.0 0.0 0.0 0.17650315863313748 0.0020197123929552433 54-55 0.0 0.0 0.0 0.20500080788495717 0.0020197123929552433 56-57 0.0 0.0 0.0 0.23874478323067283 0.0020443430318937217 58-59 0.0 0.0 0.0 0.277661192753469 0.0020936043097706785 60-61 0.0 0.0 0.0 0.32475497440384 0.002266018782340029 62-63 0.0 0.0 0.0 0.37219358499934974 0.0025123251717248146 64-65 0.0 0.0 0.0 0.42123318712586055 0.0025123251717248146 66-67 0.0 0.0 0.0 0.47702158432151454 0.0025862170885402507 68-69 0.0 0.0 0.0 0.5458395895156237 0.0026601090053556863 70-71 0.0 0.0 0.0 0.6233522102550157 0.002709370283232643 72-73 0.0 0.0 0.0 0.6995594071306686 0.002881784755801993 74-75 0.0 0.0 0.0 0.7757419733673827 0.0030295685894328648 76-77 0.0 0.0 0.0 0.8635009399051818 0.0031280911451867788 78-79 0.0 0.0 0.0 0.9626638922714966 0.003201983062002215 80-81 0.0 0.0 0.0 1.081826923455856 0.003251244339879172 82-83 0.0 0.0 0.0 1.2054481002880801 0.003423658812448522 84-85 0.0 0.0 0.0 1.327739222617626 0.0034975507292639577 86-87 0.0 0.0 0.0 1.461335808219934 0.0035714426460793933 88-89 0.0 2.4630638938478575E-5 0.0 1.6125679313021923 0.0036699652018333077 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4655 0.0 37.738018 1 GTATCAA 7420 0.0 36.055325 1 TCAACGC 8955 0.0 29.23255 4 ATCAACG 9010 0.0 29.10736 3 CAACGCA 9030 0.0 29.0946 5 TATCAAC 9225 0.0 28.584337 2 GTACCTG 1955 0.0 28.579863 1 TACATGG 9615 0.0 28.212696 2 TACCTGG 1865 0.0 28.176264 2 AACGCAG 9360 0.0 28.119404 6 GTACATG 9940 0.0 28.10545 1 ACATGGG 9975 0.0 26.338919 3 ACGCAGA 10920 0.0 24.059591 7 CGCAGAG 11130 0.0 23.479195 8 CATGGGG 5665 0.0 23.397274 4 CTTAGCG 245 7.827111E-9 21.254187 3 CGATACT 225 7.4220225E-8 21.038979 4 GTGTACG 255 1.2372766E-8 20.425722 1 AGTACAT 6960 0.0 20.40569 2 GCAGAGT 12575 0.0 20.25363 9 >>END_MODULE