FastQCFastQC Report
Thu 26 May 2016
SRR936350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936350_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029992
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64660.3185234227524049No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49700.24482855104847706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32210.15867057604167897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44200.042.181271
GTATCAA72050.037.296691
ATCAACG88550.029.9426423
TCAACGC89250.029.6553844
CAACGCA90200.029.2904645
TATCAAC92450.028.7870852
AACGCAG93400.028.3377176
TACATGG92400.026.851682
GTACATG95200.026.8283061
GTACCTG19250.025.4485111
ACATGGG96250.024.639743
ACGCAGA109800.024.0619267
CGCAGAG111300.023.6950268
TACCTGG19650.023.6594832
GTATAGG6050.022.0119511
CATGGGG54700.021.5051234
GCAGAGT125000.020.9462669
AGTACAT68700.020.8541242
ATACCCG1150.003754977820.62298
GAGTACT63700.020.43947812-13
Produced by FastQC (version 0.11.5)