##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936350_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2029992 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.483067913568135 34.0 31.0 34.0 31.0 34.0 2 32.61654134597575 34.0 31.0 34.0 31.0 34.0 3 32.69952787991283 34.0 31.0 34.0 31.0 34.0 4 36.18573373688172 37.0 35.0 37.0 35.0 37.0 5 36.148943936724876 37.0 35.0 37.0 35.0 37.0 6 36.187101722568364 37.0 37.0 37.0 35.0 37.0 7 36.1375552218925 37.0 36.0 37.0 35.0 37.0 8 36.114711289502615 37.0 36.0 37.0 35.0 37.0 9 37.8831699829359 39.0 38.0 39.0 35.0 39.0 10-11 37.818950764337984 39.0 38.0 39.0 35.0 39.0 12-13 37.76219610717678 39.0 38.0 39.0 35.0 39.0 14-15 39.10593145194661 40.5 38.5 41.0 36.0 41.0 16-17 39.035737579261394 40.0 38.0 41.0 36.0 41.0 18-19 39.07006899534579 40.0 38.5 41.0 36.0 41.0 20-21 39.026317591399376 40.0 38.5 41.0 35.0 41.0 22-23 38.90908929690363 40.0 38.0 41.0 35.0 41.0 24-25 39.051678528782375 40.0 39.0 41.0 35.0 41.0 26-27 38.97056047511518 40.0 38.5 41.0 35.0 41.0 28-29 38.88456703277649 40.0 38.0 41.0 35.0 41.0 30-31 38.760214079661395 40.0 38.0 41.0 35.0 41.0 32-33 38.59760925166208 40.0 38.0 41.0 34.5 41.0 34-35 38.45743554654402 40.0 38.0 41.0 34.0 41.0 36-37 38.307158599639806 40.0 38.0 41.0 34.0 41.0 38-39 38.17110042798198 40.0 38.0 41.0 33.5 41.0 40-41 38.01167196718016 40.0 38.0 41.0 33.0 41.0 42-43 38.01776854293021 40.0 38.0 41.0 33.0 41.0 44-45 37.86379995586189 40.0 37.0 41.0 33.0 41.0 46-47 37.63074090932378 40.0 37.0 41.0 33.0 41.0 48-49 37.36852706808697 40.0 37.0 41.0 32.5 41.0 50-51 37.14842472285605 40.0 36.5 41.0 32.0 41.0 52-53 36.91956052043555 40.0 36.0 41.0 31.0 41.0 54-55 36.70801707592936 39.0 35.5 41.0 31.0 41.0 56-57 36.44046331217069 39.0 35.0 41.0 31.0 41.0 58-59 36.16488463993947 39.0 35.0 41.0 30.0 41.0 60-61 35.86772090727452 38.0 35.0 40.0 30.0 41.0 62-63 35.55225340789521 37.5 35.0 40.0 29.5 41.0 64-65 35.16397059692846 37.0 34.5 40.0 29.0 41.0 66-67 34.805070906683376 36.5 34.0 39.5 29.0 41.0 68-69 34.44481111255611 36.0 34.0 39.0 28.0 41.0 70-71 34.069795841559966 35.5 34.0 39.0 28.0 40.0 72-73 33.66819204213613 35.0 34.0 37.5 27.5 40.0 74-75 33.252703458929886 35.0 33.0 37.0 26.5 39.0 76-77 32.54247553684941 35.0 32.5 36.0 26.0 39.0 78-79 32.60263833552054 35.0 33.0 36.0 26.0 38.5 80-81 32.45180621401464 35.0 33.0 36.0 26.0 37.0 82-83 32.18654112922613 35.0 33.0 35.0 26.0 37.0 84-85 31.916905091251593 35.0 33.0 35.0 25.5 36.5 86-87 31.53228830458445 35.0 32.0 35.0 24.5 36.0 88-89 31.051199955467805 35.0 32.0 35.0 22.0 36.0 90-91 30.845384858659543 34.0 32.0 35.0 20.5 35.5 92-93 30.57044362736405 34.0 31.0 35.0 19.5 35.0 94-95 30.419348204327896 34.0 31.0 35.0 18.5 35.0 96-97 30.16460335804279 34.0 31.0 35.0 14.0 35.0 98-99 29.717612433940626 34.0 31.0 35.0 2.0 35.0 100-101 26.389361632952248 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12952685339211456 1101 2 0.06940230500446631 1101 3 0.18459579287888062 1101 4 0.12986468041405885 1101 5 0.09775182660349202 1101 6 0.1384347423122989 1101 7 0.21227776883010563 1101 8 0.13390036485546375 1101 9 0.2786891282421067 1101 10-11 0.1834856382428356 1101 12-13 0.27839880258720484 1101 14-15 0.34995810419974305 1101 16-17 0.33999032342593694 1101 18-19 0.24792874808526477 1101 20-21 0.27372347546818787 1101 22-23 0.2688235590235024 1101 24-25 0.12351599799107049 1101 26-27 0.2254489272994249 1101 28-29 0.3035534721319877 1101 30-31 0.24980206636105606 1101 32-33 0.320771562189357 1101 34-35 0.34198356131626895 1101 36-37 0.30408609824831956 1101 38-39 0.24476021404576187 1101 40-41 0.4019284975563764 1101 42-43 0.46228152949077383 1101 44-45 0.47181322332297526 1101 46-47 0.3660805510134324 1101 48-49 0.2551007732425745 1101 50-51 0.28017045906099014 1101 52-53 0.3062398651942786 1101 54-55 0.3129396268152931 1101 56-57 0.2356135707595115 1101 58-59 0.15544156708553913 1101 60-61 0.3493516579199323 1101 62-63 0.38098987115436245 1101 64-65 0.27954191388315763 1101 66-67 0.2496082394777659 1101 68-69 0.26910340450106673 1101 70-71 0.2933247211380916 1101 72-73 0.28898689352023155 1101 74-75 0.26521935133243346 1101 76-77 0.05214876478807895 1101 78-79 0.13783930128238353 1101 80-81 0.256869329589442 1101 82-83 0.30384645611794525 1101 84-85 0.45892147034111375 1101 86-87 0.25053796607873835 1101 88-89 0.39129734572144415 1101 90-91 0.6854388593770047 1101 92-93 0.750388275941404 1101 94-95 0.546329423994905 1101 96-97 0.5495548360190234 1101 98-99 0.3325244317048224 1101 100-101 0.6965656428851759 1102 1 0.06504723645664257 1102 2 0.12183599685052826 1102 3 0.10795237397400115 1102 4 0.11790226483092425 1102 5 0.08400680425184959 1102 6 0.04397457024417406 1102 7 0.10797214716285453 1102 8 0.13933683371380567 1102 9 0.120994273262788 1102 10-11 0.0915980407535173 1102 12-13 0.1551755476307548 1102 14-15 0.1855351131333336 1102 16-17 0.155811873889121 1102 18-19 0.2860158279441265 1102 20-21 0.1686121476606175 1102 22-23 0.19591895707156226 1102 24-25 0.1043076873819544 1102 26-27 0.20490671292281348 1102 28-29 0.16054633335395607 1102 30-31 0.26534928809613945 1102 32-33 0.3059727141066446 1102 34-35 0.227020038910986 1102 36-37 0.19386543401370915 1102 38-39 0.26136013734548413 1102 40-41 0.2333431999831035 1102 42-43 0.12092598391354414 1102 44-45 0.23905954232029814 1102 46-47 0.37128817170554385 1102 48-49 0.30393054810737397 1102 50-51 0.1714701496261526 1102 52-53 0.1386541121730076 1102 54-55 0.1539423447285344 1102 56-57 0.2810350336019525 1102 58-59 0.15097669921364343 1102 60-61 0.1934331464961545 1102 62-63 0.3356058269945308 1102 64-65 0.3289026164935933 1102 66-67 0.33148385014102644 1102 68-69 0.23401222817973633 1102 70-71 0.35315976416796957 1102 72-73 0.30499550527746777 1102 74-75 0.39825609744233503 1102 76-77 0.29804395136437023 1102 78-79 0.3264596535768689 1102 80-81 0.28594766086394685 1102 82-83 0.40118433365318396 1102 84-85 0.4215631715196899 1102 86-87 0.25318505683237547 1102 88-89 0.2693861535846942 1102 90-91 0.3793244860419769 1102 92-93 0.32220580895495843 1102 94-95 0.20407422408278109 1102 96-97 0.20012119106675286 1102 98-99 0.22977738649598578 1102 100-101 0.5446400100276527 1103 1 -0.01820568944982881 1103 2 0.032997299316178896 1103 3 0.05335737530028695 1103 4 0.030869641807562687 1103 5 0.07691435246577072 1103 6 0.014282542433072365 1103 7 0.08562404986720651 1103 8 0.06308929721885903 1103 9 0.028614830380561784 1103 10-11 0.11204564157440444 1103 12-13 0.11588003882790332 1103 14-15 0.24544467474295573 1103 16-17 0.17530319787296378 1103 18-19 0.1055314542310768 1103 20-21 0.11489215716858325 1103 22-23 0.18782043378096347 1103 24-25 0.14474956964543395 1103 26-27 0.15890975008436214 1103 28-29 0.17206518658589687 1103 30-31 0.16571297707300658 1103 32-33 0.1334387323778401 1103 34-35 0.10116620912169338 1103 36-37 0.17075976878983568 1103 38-39 0.09423889860448043 1103 40-41 0.1330644124801168 1103 42-43 0.138021526727087 1103 44-45 0.14525046008612463 1103 46-47 0.3067572578293394 1103 48-49 0.17689730499947132 1103 50-51 0.17656658686467352 1103 52-53 0.20119073395870402 1103 54-55 0.1687232184401708 1103 56-57 0.254827849578092 1103 58-59 0.20688029548473708 1103 60-61 0.16003360817727952 1103 62-63 0.32062546143903603 1103 64-65 0.21711609343600458 1103 66-67 0.0211248055347113 1103 68-69 -0.11353200216851178 1103 70-71 -0.1350829005920957 1103 72-73 -0.10274029987568412 1103 74-75 0.08864882704507693 1103 76-77 0.05339100610374459 1103 78-79 0.05217434015388278 1103 80-81 0.1867681141332156 1103 82-83 0.17578391719438713 1103 84-85 0.2487900814348727 1103 86-87 0.16446176392041068 1103 88-89 0.33975024655604 1103 90-91 0.38389933196819115 1103 92-93 0.38548355703339965 1103 94-95 0.44026567737616773 1103 96-97 0.29894199696995827 1103 98-99 0.25219140692056996 1103 100-101 0.6830320744487999 1104 1 -0.045240302091762885 1104 2 -0.008944092625213784 1104 3 -0.07088183529499048 1104 4 0.021644175094579055 1104 5 0.020861254149778574 1104 6 0.05356932331719122 1104 7 -0.01439027302565421 1104 8 0.03440480921656075 1104 9 0.032610917488128166 1104 10-11 0.052106011044095624 1104 12-13 0.07758900373986677 1104 14-15 0.15433370584496942 1104 16-17 0.0898778079550624 1104 18-19 0.16842906687910641 1104 20-21 0.10624951029798524 1104 22-23 0.05702849970284518 1104 24-25 0.02103878237391399 1104 26-27 0.18026396436702896 1104 28-29 0.15537215970152118 1104 30-31 0.13539446031523283 1104 32-33 0.07284497228324938 1104 34-35 0.06942545957728186 1104 36-37 0.0732556090560621 1104 38-39 0.14958712774639338 1104 40-41 0.21894021166706779 1104 42-43 0.30640572156718804 1104 44-45 0.26222659266125703 1104 46-47 0.22194729519075196 1104 48-49 0.1731526303729254 1104 50-51 0.2369535792400157 1104 52-53 0.18332140140551445 1104 54-55 0.1732219781385993 1104 56-57 0.2344034043616361 1104 58-59 0.13647489666836066 1104 60-61 0.052683678572769566 1104 62-63 0.02411080028211643 1104 64-65 -0.13090170961507397 1104 66-67 -0.09366708587947414 1104 68-69 0.18888018630855896 1104 70-71 0.21392942928545722 1104 72-73 0.04979744247071238 1104 74-75 0.1265549724803421 1104 76-77 0.07783698441827624 1104 78-79 -0.003352925148753627 1104 80-81 -0.012811188981864063 1104 82-83 0.04118346495666003 1104 84-85 0.0720442122181133 1104 86-87 0.009779549296432322 1104 88-89 0.1151099949607115 1104 90-91 0.23269285680165197 1104 92-93 0.27645373494201664 1104 94-95 0.2956402667357807 1104 96-97 0.32780872092819635 1104 98-99 0.19043520693641725 1104 100-101 0.29824618645991663 1105 1 -0.029638103102207936 1105 2 0.0292155597159649 1105 3 0.06738675726857224 1105 4 0.029409802228116178 1105 5 0.07564957765771396 1105 6 0.04460297901859178 1105 7 0.12810502206283303 1105 8 0.12086802185481815 1105 9 0.10889252351041279 1105 10-11 0.05616722535652485 1105 12-13 0.09865231374158867 1105 14-15 0.09452611743122219 1105 16-17 0.16140730493026467 1105 18-19 0.13310750027714846 1105 20-21 0.20392124698916803 1105 22-23 -0.012484118958880686 1105 24-25 0.1491708879776681 1105 26-27 0.22566235312035587 1105 28-29 0.16293489680234785 1105 30-31 0.19127545128535672 1105 32-33 0.2228031178653822 1105 34-35 0.19323498338680878 1105 36-37 0.029819628505002527 1105 38-39 0.09372157244809642 1105 40-41 0.12812621744951258 1105 42-43 0.1674187765834816 1105 44-45 0.1570258101914348 1105 46-47 0.11730486846567345 1105 48-49 0.0984457597179258 1105 50-51 0.19130925867673199 1105 52-53 0.19329150963331898 1105 54-55 0.12537659013923985 1105 56-57 0.05797783101547793 1105 58-59 0.20945074577872447 1105 60-61 0.196513417175602 1105 62-63 0.18432327824175587 1105 64-65 0.10044353886955548 1105 66-67 -0.05111657858017793 1105 68-69 0.01031929555156097 1105 70-71 0.07587515508855347 1105 72-73 0.160684785813487 1105 74-75 0.28828593465700436 1105 76-77 0.14108925079047907 1105 78-79 0.08019220341257949 1105 80-81 0.11479595535948306 1105 82-83 0.17671519416748538 1105 84-85 0.24232238083502367 1105 86-87 0.23958687055431938 1105 88-89 0.31093998884581353 1105 90-91 0.4904109895603028 1105 92-93 0.6137911409179999 1105 94-95 0.4264583276591907 1105 96-97 0.3810692097640498 1105 98-99 0.24910812505017788 1105 100-101 0.6011241971580255 1106 1 -0.07743486030108215 1106 2 -0.054860722121176764 1106 3 -0.10822419772960501 1106 4 -0.11987279502017856 1106 5 -0.15092415601201736 1106 6 0.007249222753550555 1106 7 -4.390950523358583E-4 1106 8 -0.06697185839626485 1106 9 -0.09577195425267604 1106 10-11 0.0245554250986757 1106 12-13 -0.09523752397596041 1106 14-15 0.05885704228712285 1106 16-17 -4.875462479319026E-4 1106 18-19 0.041483687535198044 1106 20-21 -0.002710994142233858 1106 22-23 0.02346234261270297 1106 24-25 0.0073207660728726864 1106 26-27 -0.07043754814260694 1106 28-29 -0.16775443694906045 1106 30-31 -0.09652435887296207 1106 32-33 -0.007662711914477427 1106 34-35 0.01312603944022328 1106 36-37 -0.02337064292075297 1106 38-39 0.0740204435081182 1106 40-41 -0.18584061636099136 1106 42-43 -0.05244428451528904 1106 44-45 -0.020978670710057656 1106 46-47 0.043747911548571494 1106 48-49 -0.04912951552545053 1106 50-51 -0.03440098870127173 1106 52-53 -0.12218628279220667 1106 54-55 -0.060674660278337456 1106 56-57 0.06357497080257701 1106 58-59 0.02780635119169972 1106 60-61 -0.13219021137492604 1106 62-63 -0.1473806234233379 1106 64-65 -0.0665480743204796 1106 66-67 -0.073098171399117 1106 68-69 -0.04884233102650626 1106 70-71 -0.19339387471681135 1106 72-73 -0.23011922757511627 1106 74-75 -0.22249311964893792 1106 76-77 -0.26796103674875127 1106 78-79 -0.40824627902735244 1106 80-81 -0.1849341766952577 1106 82-83 -0.2677010085034581 1106 84-85 -0.0854585248971631 1106 86-87 -0.07300399557198389 1106 88-89 0.0488434202070529 1106 90-91 -0.005241533531265219 1106 92-93 0.061882144171161 1106 94-95 0.08133934625404748 1106 96-97 0.04563241015409858 1106 98-99 0.04637880208665379 1106 100-101 0.3627843235995307 1107 1 -0.009379537089891699 1107 2 -0.0028829758278945405 1107 3 -0.009824487734576337 1107 4 0.002553756310909705 1107 5 0.05815402273088921 1107 6 -0.02193737856988065 1107 7 -0.021811379526511132 1107 8 0.07975730947703852 1107 9 0.03600537579055896 1107 10-11 0.027809601931728878 1107 12-13 0.08990965854517441 1107 14-15 0.19118856877345536 1107 16-17 0.180789963284397 1107 18-19 0.02916465031407256 1107 20-21 0.0329655856336899 1107 22-23 -0.03906145328894439 1107 24-25 0.02213339328448427 1107 26-27 0.019785485790386304 1107 28-29 0.10029303637086429 1107 30-31 -0.03505809326616571 1107 32-33 0.038914485592023595 1107 34-35 0.030193525866813786 1107 36-37 0.03159359009342211 1107 38-39 -1.2604251892156526E-4 1107 40-41 0.08041064307614931 1107 42-43 -0.14067970834032906 1107 44-45 -0.12449021813530692 1107 46-47 -0.03255112050397457 1107 48-49 -0.07030084866337205 1107 50-51 0.030769425969971564 1107 52-53 -0.07851722009568363 1107 54-55 -0.022293396057762038 1107 56-57 -0.03314744969229366 1107 58-59 -0.016262703155135227 1107 60-61 0.0894902768824366 1107 62-63 0.08856505768115852 1107 64-65 0.07409007773867415 1107 66-67 -0.018204205201868717 1107 68-69 0.22199170669001944 1107 70-71 0.2545360619166317 1107 72-73 0.22844348967504402 1107 74-75 0.14583553854004805 1107 76-77 0.09364832032164117 1107 78-79 0.11090260459756962 1107 80-81 0.26602546516154035 1107 82-83 0.4166135563376727 1107 84-85 0.26042929339461196 1107 86-87 0.3410214668880407 1107 88-89 0.44907909334698815 1107 90-91 0.09888433403607166 1107 92-93 0.41039555998813526 1107 94-95 0.31653884668343935 1107 96-97 0.11388001160071326 1107 98-99 0.022374893017207853 1107 100-101 0.5288574463656559 1108 1 -0.08055408469470393 1108 2 -0.021721576185825597 1108 3 0.021323248777562753 1108 4 -0.026429438039279773 1108 5 -0.0012259959495182216 1108 6 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-0.5447763674049924 2316 60-61 -0.7861023053819238 2316 62-63 -0.7502002797421525 2316 64-65 -0.6593699055295872 2316 66-67 -0.6419841828928909 2316 68-69 -0.569367049584379 2316 70-71 -0.7860558231921289 2316 72-73 -0.9899753614125189 2316 74-75 -0.838931611487844 2316 76-77 -0.6638635313236492 2316 78-79 -0.9203648242925553 2316 80-81 -0.9376485448774972 2316 82-83 -1.063866086718658 2316 84-85 -0.9057203161982521 2316 86-87 -1.003582548728243 2316 88-89 -0.7914395904232272 2316 90-91 -0.8556262627291247 2316 92-93 -0.9196204745907828 2316 94-95 -0.8785427205223613 2316 96-97 -0.750157216508601 2316 98-99 -0.8233794293475434 2316 100-101 -0.747268459936631 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 25.0 5 31.0 6 62.0 7 155.0 8 342.0 9 613.0 10 1083.0 11 1739.0 12 2638.0 13 4004.0 14 5100.0 15 7031.0 16 9132.0 17 12908.0 18 12056.0 19 7922.0 20 6915.0 21 7085.0 22 7772.0 23 8297.0 24 9538.0 25 11954.0 26 14887.0 27 18282.0 28 23428.0 29 28659.0 30 35850.0 31 44518.0 32 56608.0 33 73311.0 34 101509.0 35 154795.0 36 270355.0 37 493189.0 38 508475.0 39 89660.0 40 57.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.28208689673295 17.94234479515618 13.234853925724543 23.540714382386327 2 16.14815420666657 18.97429429026975 39.73328577031398 25.144265732749698 3 20.31564656412439 23.431865741342822 28.118337412167143 28.134150282365646 4 13.076258428604644 14.9653299126302 35.1911731671849 36.767238491580265 5 14.295672101170842 37.3037430689382 33.224712215614645 15.175872614276312 6 34.84003877847795 34.20279488786163 16.26769957714119 14.689466756519238 7 30.993077805232733 31.43401550350937 19.05027211929899 18.522634571958903 8 27.880898052800205 35.11501523158712 18.124406401601583 18.87968031401109 9 27.256363571876147 15.866909820334268 18.963276702568287 37.913449905221306 10-11 26.52870009598595 25.75864495517084 25.74620643655161 21.9664485122916 12-13 26.860649559850046 22.53474155045099 26.97456637717427 23.630042512524692 14-15 23.559945063822912 24.078321490922132 24.936280537066153 27.425452908188802 16-17 23.50329301030576 27.453427531236123 25.493434299770712 23.549845158687408 18-19 23.395732594020075 25.925619411308027 27.628015282818847 23.05063271185305 20-21 24.154915995912297 25.093393294205075 27.76825802879373 22.983432681088903 22-23 23.722433388900054 24.91630508888705 27.787203102278234 23.57405841993466 24-25 23.504960230469184 25.146555622661882 27.99232097799646 23.356163168872474 26-27 23.33654687876395 25.43061426666384 27.81675795795855 23.416080896613657 28-29 23.18782393055195 25.578278074966416 27.903927635620402 23.32997035886123 30-31 23.299286886715677 25.43788781323092 27.72508684848855 23.53773845156485 32-33 23.235417779679292 25.410604673433784 27.803406317404228 23.5505712294827 34-35 23.5799806252674 25.475310270357078 27.727542884391767 23.21716621998376 36-37 23.72538196607324 25.290192244742034 27.613035627506633 23.371390161678097 38-39 23.587651749665515 25.43305674713348 27.639079639879643 23.340211863321358 40-41 23.508758101998666 25.38776907625823 27.79112083754623 23.31235198419687 42-43 23.50391371452865 25.399807775013016 27.473591232727706 23.622687277730623 44-45 23.464693189879405 25.635566918893353 27.39873985181682 23.501000039410417 46-47 23.701224799479732 25.838539305914114 27.08043473976706 23.379801154839093 48-49 23.889164104533553 25.819946234003037 26.774018011830687 23.51687164963272 50-51 23.803565195779345 26.082449924859887 26.61669477595782 23.497290103402943 52-53 23.96948354957199 25.950141290810773 26.635436208282755 23.44493895133448 54-55 23.77253312650078 25.952723261051137 26.58930478991578 23.6854388225323 56-57 23.596682660571247 26.296294471757786 26.553173985437994 23.553848882232973 58-59 23.7205813893343 25.968772860615825 26.680139805366103 23.630505944683776 60-61 23.64972974890195 26.00253009412975 26.7913618172539 23.5563783397144 62-63 23.64287642512877 25.998501471926982 26.85907136579849 23.499550737145764 64-65 23.59027915471553 25.924011098699857 26.809419362625263 23.67629038395935 66-67 23.530117360068413 26.241162526748873 26.591139275425718 23.637580837756996 68-69 23.536595218109234 26.113772862159063 26.81599237829509 23.533639541436617 70-71 23.59689545192229 25.883212696420347 26.68033504606797 23.83955680558939 72-73 23.607004932832734 26.240788346819986 26.405716933185445 23.746489787161824 74-75 23.52824543150909 26.39099070341164 26.326704735782208 23.75405912929706 76-77 23.713179176514636 26.301245724047334 26.296359273557567 23.68921582588047 78-79 23.636771143441997 26.36569452094853 26.398660453849914 23.59887388175956 80-81 23.584086795713414 26.420198998798995 26.239407437183555 23.756306768304036 82-83 23.702827400305026 26.523478910261716 26.1996352694986 23.57405841993466 84-85 23.67669682956999 26.454610076862938 26.044235969176004 23.824457124391067 86-87 23.66445440313768 26.299861010137676 26.280477399104115 23.75520718762053 88-89 23.683709143902114 26.36508664442302 26.15488940240706 23.796314809267805 90-91 23.518140909754635 26.4516050925545 26.232427294665044 23.79782670302582 92-93 23.475887664663915 26.458772943513665 26.225972025353165 23.839367366469258 94-95 23.585188454752558 26.312185536198424 26.250853779920476 23.851772229128546 96-97 23.671138468207843 26.12689768306245 26.19155468689384 24.010409161835867 98-99 23.406575894070013 26.215593579036273 26.084727932216868 24.29310259467685 100-101 23.37556201320309 26.313400286406903 26.140738401727308 24.170299298662695 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 148.0 1 120.0 2 181.0 3 303.5 4 257.5 5 239.0 6 524.5 7 1061.0 8 1700.5 9 2015.5 10 1750.0 11 1471.0 12 1496.0 13 1659.5 14 1815.5 15 1854.0 16 1923.5 17 2058.5 18 2243.5 19 2385.0 20 2544.5 21 2969.5 22 3563.5 23 4305.0 24 5150.5 25 5999.0 26 7617.5 27 9831.0 28 11633.5 29 13619.0 30 16147.5 31 19410.5 32 23580.5 33 26754.0 34 28963.5 35 31471.0 36 35510.0 37 39649.0 38 42771.0 39 46201.0 40 48634.5 41 51622.5 42 54224.5 43 56572.5 44 60404.5 45 64520.5 46 68456.5 47 72201.5 48 77536.0 49 80494.0 50 79122.0 51 78844.0 52 80239.5 53 82287.5 54 84333.0 55 80735.0 56 73800.5 57 67597.0 58 63503.5 59 59238.0 60 51673.5 61 43786.5 62 36419.0 63 28560.0 64 21154.5 65 15755.5 66 11205.5 67 8490.0 68 6662.0 69 5018.0 70 3955.0 71 2815.5 72 2040.0 73 1749.0 74 1370.0 75 897.5 76 592.5 77 322.5 78 154.0 79 70.5 80 36.5 81 22.0 82 18.0 83 12.5 84 9.0 85 5.5 86 3.0 87 2.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2696562350984635 2 0.01990155626229069 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.6945958407717865E-4 12-13 9.85225557539143E-5 14-15 0.0 16-17 5.1724341770805E-4 18-19 0.0 20-21 7.389191681543572E-5 22-23 0.0 24-25 0.002266018782340029 26-27 0.0021182349487091573 28-29 0.0021182349487091573 30-31 9.85225557539143E-4 32-33 0.0014039464194932788 34-35 0.0014039464194932788 36-37 7.389191681543572E-5 38-39 1.970451115078286E-4 40-41 7.142885292158787E-4 42-43 0.003891640952279615 44-45 0.003546812007140915 46-47 0.013399067582532344 48-49 0.013325175665716909 50-51 0.005788200150542465 52-53 0.006699533791266172 54-55 4.1872086195413575E-4 56-57 0.002561586449601772 58-59 0.0013300545026778431 60-61 0.0013793157805548001 62-63 0.0 64-65 0.00120690130798545 66-67 0.0 68-69 0.0 70-71 1.2315319469239288E-4 72-73 0.0011083787522315359 74-75 0.0 76-77 0.19613377786710492 78-79 0.10546839593456526 80-81 0.0014778383363087146 82-83 0.0 84-85 7.389191681543573E-4 86-87 0.12344876235965462 88-89 0.5855934407623281 90-91 0.30997659104075287 92-93 0.2928582969785103 94-95 0.002389171977032422 96-97 0.002438433254909379 98-99 0.0023399106991554646 100-101 6.40396612400443E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2029992.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.2094435126889 #Duplication Level Percentage of deduplicated Percentage of total 1 68.22243670693742 30.842983986002814 2 15.357098419386537 13.885717470201195 3 6.187910773462739 8.392560077232684 4 3.1791457378717936 5.749096386196821 5 1.8316478341633433 4.140388964687332 6 1.1424399908422196 3.0989445739570893 7 0.7816134993706588 2.473541794398703 8 0.5653878425957778 2.044869578607589 9 0.4186041954497478 1.7032376455523963 >10 2.1137501290868665 17.02701000056751 >50 0.13669649656409755 4.248953061623117 >100 0.060433199082797026 4.797586822555851 >500 0.0021809039891997027 0.690231488672106 >1k 5.452259972999252E-4 0.5861125754462299 >5k 1.0904519945998504E-4 0.3187655742985383 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6466 0.3185234227524049 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4970 0.24482855104847706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3221 0.15867057604167897 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.9556766726174293E-4 2 0.0 0.0 0.0 0.0 2.9556766726174293E-4 3 0.0 0.0 0.0 0.0 2.9556766726174293E-4 4 0.0 0.0 0.0 0.0 2.9556766726174293E-4 5 0.0 0.0 0.0 0.0 3.4482894513870004E-4 6 0.0 0.0 0.0 0.0 3.4482894513870004E-4 7 0.0 0.0 0.0 0.0 3.4482894513870004E-4 8 0.0 0.0 0.0 0.0 3.4482894513870004E-4 9 0.0 0.0 0.0 0.0 3.4482894513870004E-4 10-11 0.0 0.0 0.0 4.926127787695715E-5 3.4482894513870004E-4 12-13 0.0 0.0 0.0 9.85225557539143E-5 6.157659734619644E-4 14-15 0.0 0.0 0.0 9.85225557539143E-5 8.12811084969793E-4 16-17 0.0 0.0 0.0 1.7241447256935005E-4 8.867030017852287E-4 18-19 0.0 0.0 0.0 2.4630638938478576E-4 8.867030017852287E-4 20-21 0.0 0.0 0.0 5.172434177080501E-4 0.0011330093911700145 22-23 0.0 0.0 0.0 0.001280793224800886 0.0011822706690469717 24-25 0.0 0.0 0.0 0.0019458204761398075 0.0012315319469239287 26-27 4.926127787695715E-5 0.0 0.0 0.0020689736708322 0.0013054238637393645 28-29 4.926127787695715E-5 0.0 0.0 0.002536955810663293 0.0013300545026778431 30-31 4.926127787695715E-5 0.0 0.0 0.004236469897418315 0.0013300545026778431 32-33 4.926127787695715E-5 0.0 0.0 0.00657638059657378 0.0014778383363087146 34-35 4.926127787695715E-5 0.0 0.0 0.01002467004796078 0.0016009915310011074 36-37 4.926127787695715E-5 0.0 0.0 0.017019771506488694 0.001625622169939586 38-39 4.926127787695715E-5 0.0 0.0 0.02987696503237451 0.001674883447816543 40-41 4.926127787695715E-5 0.0 0.0 0.04270952791932185 0.001674883447816543 42-43 4.926127787695715E-5 0.0 0.0 0.05815293853374792 0.001674883447816543 44-45 4.926127787695715E-5 0.0 0.0 0.07492640365085182 0.0017487753646319788 46-47 4.926127787695715E-5 0.0 0.0 0.09699545613972863 0.001921189837201329 48-49 4.926127787695715E-5 0.0 0.0 0.12224186105166918 0.0020443430318937217 50-51 4.926127787695715E-5 0.0 0.0 0.15051783455304257 0.0020689736708322 52-53 4.926127787695715E-5 0.0 0.0 0.17775932121899987 0.002266018782340029 54-55 4.926127787695715E-5 0.0 0.0 0.20647864622126588 0.002266018782340029 56-57 4.926127787695715E-5 0.0 0.0 0.24073986498468958 0.002266018782340029 58-59 4.926127787695715E-5 0.0 0.0 0.2800503647305014 0.002266018782340029 60-61 4.926127787695715E-5 0.0 0.0 0.32702099318618005 0.002266018782340029 62-63 4.926127787695715E-5 0.0 0.0 0.3748536940047055 0.0023645413380939434 64-65 4.926127787695715E-5 0.0 0.0 0.4239918186869702 0.0024630638938478574 66-67 4.926127787695715E-5 0.0 0.0 0.4798294771605011 0.0025123251717248146 68-69 4.926127787695715E-5 0.0 0.0 0.5485982210767333 0.002561586449601772 70-71 4.926127787695715E-5 0.0 0.0 0.6260123192603715 0.002610847727478729 72-73 4.926127787695715E-5 0.0 0.0 0.7020471016634549 0.0027586315611096007 74-75 4.926127787695715E-5 0.0 0.0 0.7781065147054766 0.002906415394740472 76-77 4.926127787695715E-5 0.0 0.0 0.8670231212733843 0.002955676672617429 78-79 4.926127787695715E-5 0.0 0.0 0.9664570106680224 0.0030049379504943864 80-81 4.926127787695715E-5 0.0 0.0 1.085841717602828 0.0030049379504943864 82-83 4.926127787695715E-5 0.0 0.0 1.2102510748810833 0.0031034605062483004 84-85 4.926127787695715E-5 0.0 0.0 1.3332318551009068 0.0031034605062483004 86-87 4.926127787695715E-5 0.0 0.0 1.4679614500943847 0.003201983062002215 88-89 7.389191681543572E-5 0.0 0.0 1.6200063842616128 0.0033005056177561293 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4420 0.0 42.18127 1 GTATCAA 7205 0.0 37.29669 1 ATCAACG 8855 0.0 29.942642 3 TCAACGC 8925 0.0 29.655384 4 CAACGCA 9020 0.0 29.290464 5 TATCAAC 9245 0.0 28.787085 2 AACGCAG 9340 0.0 28.337717 6 TACATGG 9240 0.0 26.85168 2 GTACATG 9520 0.0 26.828306 1 GTACCTG 1925 0.0 25.448511 1 ACATGGG 9625 0.0 24.63974 3 ACGCAGA 10980 0.0 24.061926 7 CGCAGAG 11130 0.0 23.695026 8 TACCTGG 1965 0.0 23.659483 2 GTATAGG 605 0.0 22.011951 1 CATGGGG 5470 0.0 21.505123 4 GCAGAGT 12500 0.0 20.946266 9 AGTACAT 6870 0.0 20.854124 2 ATACCCG 115 0.0037549778 20.6229 8 GAGTACT 6370 0.0 20.439478 12-13 >>END_MODULE