##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936349_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 221915 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.770258882905615 33.0 31.0 34.0 30.0 34.0 2 31.83844264695942 33.0 31.0 34.0 30.0 34.0 3 31.879444832480903 34.0 31.0 34.0 30.0 34.0 4 35.39315954306829 37.0 35.0 37.0 33.0 37.0 5 35.430385507964765 37.0 35.0 37.0 33.0 37.0 6 35.57077259310997 37.0 35.0 37.0 35.0 37.0 7 35.531766667417706 37.0 35.0 37.0 35.0 37.0 8 35.55905639546673 37.0 35.0 37.0 35.0 37.0 9 37.23041254534394 39.0 37.0 39.0 34.0 39.0 10-11 37.18883356240002 39.0 37.0 39.0 34.5 39.0 12-13 37.14544307505126 39.0 37.0 39.0 34.0 39.0 14-15 38.27966563774418 40.0 38.0 41.0 34.0 41.0 16-17 38.24203861838993 40.0 38.0 41.0 34.0 41.0 18-19 38.329871797760404 40.0 38.0 41.0 34.0 41.0 20-21 38.30117161976432 40.0 38.0 41.0 34.0 41.0 22-23 38.15194556474326 40.0 38.0 41.0 34.0 41.0 24-25 38.265051934299166 40.0 38.0 41.0 34.0 41.0 26-27 38.1417569790235 40.0 38.0 41.0 34.0 41.0 28-29 37.976448189622154 40.0 38.0 41.0 34.0 41.0 30-31 37.76523443660861 40.0 37.0 41.0 33.5 41.0 32-33 37.45870716265237 40.0 36.5 41.0 33.0 41.0 34-35 37.15512470991145 40.0 36.0 41.0 33.0 41.0 36-37 36.8997273730933 40.0 35.5 41.0 32.5 41.0 38-39 36.66392312371854 40.0 35.0 41.0 31.5 41.0 40-41 36.44566613342947 40.0 35.0 41.0 31.0 41.0 42-43 36.183896987585335 40.0 35.0 41.0 30.0 41.0 44-45 35.86964828875921 40.0 35.0 41.0 29.5 41.0 46-47 35.39244079940518 40.0 35.0 41.0 26.0 41.0 48-49 35.03686546650745 39.5 35.0 41.0 23.5 41.0 50-51 34.620030191740085 39.5 35.0 40.5 22.5 41.0 52-53 34.59180091476466 39.0 35.0 40.5 20.0 41.0 54-55 34.68134195525314 39.0 35.0 41.0 18.5 41.0 56-57 34.569965977964536 39.0 35.0 41.0 13.5 41.0 58-59 34.38620643039002 39.0 35.0 41.0 7.5 41.0 60-61 34.1168735777212 39.0 35.0 41.0 2.0 41.0 62-63 33.810055651938804 39.0 35.0 41.0 2.0 41.0 64-65 33.484816258477345 38.0 34.0 40.0 2.0 41.0 66-67 33.146675528918735 37.5 34.0 40.0 2.0 41.0 68-69 32.781145934254106 37.0 34.0 40.0 2.0 41.0 70-71 32.33947006736814 36.5 33.5 39.0 2.0 41.0 72-73 31.880792195209878 36.0 33.0 39.0 2.0 41.0 74-75 31.44109231011874 35.0 33.0 38.5 2.0 40.0 76-77 30.99983777572494 35.0 32.0 37.0 2.0 39.5 78-79 30.565347092355182 35.0 31.5 37.0 2.0 39.0 80-81 30.15872744068675 35.0 31.0 36.0 2.0 39.0 82-83 29.782763670774848 35.0 31.0 36.0 2.0 37.5 84-85 29.402674447423564 35.0 31.0 35.5 2.0 37.0 86-87 29.054948966946803 35.0 30.5 35.0 2.0 37.0 88-89 28.640387085145214 34.5 29.5 35.0 2.0 36.0 90-91 28.25428880427191 34.0 29.0 35.0 2.0 36.0 92-93 27.935096771286304 34.0 29.0 35.0 2.0 36.0 94-95 27.603251244846 34.0 29.0 35.0 2.0 35.0 96-97 27.204006038348016 34.0 27.5 35.0 2.0 35.0 98-99 26.7703580199626 34.0 26.5 35.0 2.0 35.0 100-101 23.946858031228174 30.0 13.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45376995356303595 1101 2 0.37794778746129865 1101 3 0.42056612848591257 1101 4 0.1084342094589772 1101 5 0.5838188896031582 1101 6 0.39442605299918654 1101 7 0.33786303966580533 1101 8 0.5185257419492757 1101 9 0.7967915948997515 1101 10-11 0.653252159166982 1101 12-13 0.6610120802454844 1101 14-15 0.7851223729051142 1101 16-17 0.7904046179931541 1101 18-19 0.5179340966253818 1101 20-21 0.49553535131123283 1101 22-23 0.524749589388442 1101 24-25 0.2768442453461901 1101 26-27 0.12628081639501687 1101 28-29 -0.09132030761657717 1101 30-31 0.09183898198570262 1101 32-33 0.16832616694260594 1101 34-35 -0.07989762824929869 1101 36-37 -0.05697816261749722 1101 38-39 0.05937539177076445 1101 40-41 -0.0037988553238150757 1101 42-43 0.07461037638038448 1101 44-45 0.15323753171637122 1101 46-47 0.3579758752968729 1101 48-49 0.45064525832624014 1101 50-51 1.012616846642345 1101 52-53 0.9773530675335422 1101 54-55 1.1677559049222381 1101 56-57 1.4725911761009272 1101 58-59 1.4736449353164787 1101 60-61 1.147258409968245 1101 62-63 0.9251900867207681 1101 64-65 1.1638780643825157 1101 66-67 0.9674457150529321 1101 68-69 0.9865620712014191 1101 70-71 1.431437058337977 1101 72-73 1.5558773166035884 1101 74-75 1.3128689283184762 1101 76-77 1.5473871742069605 1101 78-79 1.6238483548521145 1101 80-81 1.6051826832998337 1101 82-83 1.7167786553452054 1101 84-85 2.0911800132839 1101 86-87 2.093548448800753 1101 88-89 2.212539800497211 1101 90-91 1.8265429993279234 1101 92-93 1.9755556472818014 1101 94-95 2.4555974512626157 1101 96-97 2.00256737290173 1101 98-99 2.021192820377248 1101 100-101 2.267339268034334 1102 1 0.4284952282883161 1102 2 0.5236620731755828 1102 3 0.48957711749689636 1102 4 0.8194232204479945 1102 5 0.6418408676251346 1102 6 0.5071733057464414 1102 7 0.497863039665809 1102 8 0.5438004672240027 1102 9 0.40931906742722646 1102 10-11 0.6555598514746706 1102 12-13 0.6392538384872353 1102 14-15 0.866111383894129 1102 16-17 1.0529320905206205 1102 18-19 0.8793626680539575 1102 20-21 0.980040845816724 1102 22-23 0.9622221168609641 1102 24-25 0.9710200695220195 1102 26-27 0.6350720251862256 1102 28-29 0.6203280440317727 1102 30-31 0.9163444764911901 1102 32-33 0.6265679251843679 1102 34-35 0.7302122618605935 1102 36-37 1.0337910681517286 1102 38-39 1.063661106056479 1102 40-41 0.6734538919289363 1102 42-43 0.7014235631935648 1102 44-45 0.9009298394086755 1102 46-47 0.8909429082639093 1102 48-49 1.242403500084471 1102 50-51 1.1330564070819023 1102 52-53 0.685704715885187 1102 54-55 0.9945690917354213 1102 56-57 0.8853384288481791 1102 58-59 1.001557023228564 1102 60-61 1.2138518165616574 1102 62-63 1.329695581226261 1102 64-65 1.4313505918550433 1102 66-67 1.3203028579100788 1102 68-69 1.225023609662962 1102 70-71 1.1893491462500592 1102 72-73 1.128624569350837 1102 74-75 0.8330887085382663 1102 76-77 0.7900245368443173 1102 78-79 0.9296725306762958 1102 80-81 0.9521057602229135 1102 82-83 0.8827127212792689 1102 84-85 1.3029382550421431 1102 86-87 1.0912407564930575 1102 88-89 1.3166057345631437 1102 90-91 1.4117078344927556 1102 92-93 1.7391820209081779 1102 94-95 1.7424106380757998 1102 96-97 1.2905893509237067 1102 98-99 1.4864675456519763 1102 100-101 1.402284322979387 1103 1 -0.25587977171168674 1103 2 -0.3467545934910845 1103 3 -0.3032205015507188 1103 4 -0.213910112885344 1103 5 -0.1256591323748637 1103 6 -0.31190407520593766 1103 7 -0.31094648414371306 1103 8 -0.3960804851569506 1103 9 -0.2775559325727741 1103 10-11 0.06505389909371218 1103 12-13 -0.07925806627466869 1103 14-15 -0.3396773065820611 1103 16-17 -0.2910113618603347 1103 18-19 -0.3540897128984213 1103 20-21 -0.2923252256118474 1103 22-23 -0.18736121647236104 1103 24-25 -0.14079540666845247 1103 26-27 -0.058157141480435826 1103 28-29 -0.08788624168251147 1103 30-31 -0.1684918330326184 1103 32-33 -0.24939933672039416 1103 34-35 -0.2106210714727368 1103 36-37 -0.13722083661017592 1103 38-39 0.20781289177076445 1103 40-41 0.5924122252622652 1103 42-43 0.3187152298602385 1103 44-45 0.08183757750391862 1103 46-47 0.2160917177877124 1103 48-49 -0.05350423801076687 1103 50-51 -0.006928711965713319 1103 52-53 -0.019384569829092868 1103 54-55 0.22473278221161053 1103 56-57 0.24728783361008055 1103 58-59 0.32085761846666827 1103 60-61 0.3983458641806976 1103 62-63 0.3612878431310236 1103 64-65 0.39261547280742803 1103 66-67 0.4440082150529321 1103 68-69 -0.16917281890847136 1103 70-71 -0.09597823470232214 1103 72-73 -0.1307355496967837 1103 74-75 -0.16174760098554586 1103 76-77 -0.15507962982234602 1103 78-79 0.05153264972391369 1103 80-81 0.1735492126038629 1103 82-83 0.033084745088793 1103 84-85 -0.084844483053093 1103 86-87 0.11813063744544294 1103 88-89 0.39176942503933176 1103 90-91 0.4254727154451352 1103 92-93 0.6203873780510349 1103 94-95 0.33214278093294425 1103 96-97 0.3049941128284708 1103 98-99 0.563431831366259 1103 100-101 0.4212426563127245 1104 1 0.7441202282883133 1104 2 0.856370406508919 1104 3 0.8321961651159491 1104 4 0.7183815537813274 1104 5 0.895174200958472 1104 6 0.44330425812739094 1104 7 0.7150951825229512 1104 8 0.781002848176378 1104 9 1.0453607340938902 1104 10-11 1.0051580657603836 1104 12-13 0.9780336003919956 1104 14-15 1.0327185267512746 1104 16-17 0.8782594714730081 1104 18-19 1.023514453768243 1104 20-21 0.999341441054824 1104 22-23 1.0418054501942962 1104 24-25 1.2055587599982118 1104 26-27 1.066842858519557 1104 28-29 1.0761762583174885 1104 30-31 0.9070290003007244 1104 32-33 0.9120589966129344 1104 34-35 0.8154205951939204 1104 36-37 0.9461124967231598 1104 38-39 0.9994795584374359 1104 40-41 1.4439747252622581 1104 42-43 1.362986063193567 1104 44-45 1.1964209108372472 1104 46-47 1.3124458844543767 1104 48-49 1.0037874286559045 1104 50-51 1.1415087880342938 1104 52-53 1.1941570968375785 1104 54-55 1.2716077822116105 1104 56-57 1.2863503336100877 1104 58-59 1.3156492851333255 1104 60-61 1.463450030847369 1104 62-63 1.4628503431310165 1104 64-65 1.650427972807428 1104 66-67 1.5247373817195964 1104 68-69 1.4948896810915286 1104 70-71 1.4352717652976779 1104 72-73 1.5593686169698877 1104 74-75 1.5075232323477898 1104 76-77 1.4647120368443183 1104 78-79 1.5124701497239101 1104 80-81 1.775111712603863 1104 82-83 1.7257930784221287 1104 84-85 1.5349471836135713 1104 86-87 1.8004223041121108 1104 88-89 1.8318735917059996 1104 90-91 1.930681048778471 1104 92-93 1.7193457113843706 1104 94-95 1.9076636142662764 1104 96-97 2.065410779495135 1104 98-99 2.7483276646995947 1104 100-101 2.741555156312721 1105 1 0.1868285616216454 1105 2 0.24699540650891905 1105 3 0.20198783178261337 1105 4 -0.015993446218672602 1105 5 0.1399658676251363 1105 6 -0.0046124085392733605 1105 7 0.09530351585628694 1105 8 0.0257945148430494 1105 9 0.3891107340938902 1105 10-11 0.09109556576038358 1105 12-13 -0.253737232941333 1105 14-15 0.06917686008460322 1105 16-17 0.3730511381396653 1105 18-19 0.3177852871015716 1105 20-21 -0.11784605894517597 1105 22-23 0.007951283527631858 1105 24-25 -0.07569124000179528 1105 26-27 -0.016490474813771527 1105 28-29 -0.046219575015840064 1105 30-31 0.021612333634053016 1105 32-33 0.16205899661293444 1105 34-35 0.3284414285272632 1105 36-37 0.4825708300564884 1105 38-39 0.7494795584374359 1105 40-41 0.8189747252622652 1105 42-43 0.7692360631935671 1105 44-45 0.6860042441705829 1105 46-47 0.3436958844543767 1105 48-49 0.6183707619892331 1105 50-51 0.8889046213676224 1105 52-53 0.6212404301709071 1105 54-55 0.7299411155449462 1105 56-57 0.8488503336100877 1105 58-59 1.0057534517999969 1105 60-61 0.9269916975140404 1105 62-63 0.3248295097976879 1105 64-65 0.21553213947408523 1105 66-67 0.24609154838626068 1105 68-69 0.23968134775820005 1105 70-71 0.23475093196434926 1105 72-73 -0.08646471636344799 1105 74-75 -0.31018510098554586 1105 76-77 0.01679537017765398 1105 78-79 0.1895534830572494 1105 80-81 0.045945045937198614 1105 82-83 -0.0242069215778713 1105 84-85 -0.056198649719764404 1105 86-87 -0.1422860292212249 1105 88-89 -0.04573057496066468 1105 90-91 0.21713938211180306 1105 92-93 0.40163737805103494 1105 94-95 0.1706844475996121 1105 96-97 0.4195774461618029 1105 98-99 0.5582234980329268 1105 100-101 -0.011049010353946898 1106 1 0.1880911878842717 1106 2 0.04244995196346224 1106 3 0.23497394289372764 1106 4 -0.00683940581463105 1106 5 0.19315399893826424 1106 6 0.19551385408698962 1106 7 0.22819492999769864 1106 8 0.0388942623177968 1106 9 0.18851098661914278 1106 10-11 0.25429001020482644 1106 12-13 0.0220676913010891 1106 14-15 -0.11658702880428251 1106 16-17 -5.492246638993947E-5 1106 18-19 0.06447089316218069 1106 20-21 -0.08138772561184737 1106 22-23 0.46636353100237926 1106 24-25 0.4960417145436651 1106 26-27 0.36537505548925964 1106 28-29 0.3129975967013223 1106 30-31 0.2035883437350634 1106 32-33 0.3363803349967682 1106 34-35 0.18900014064846715 1106 36-37 0.2538776482383156 1106 38-39 0.1957390281344047 1106 40-41 0.6727468212218568 1106 42-43 0.4255649773349788 1106 44-45 0.2116513401301745 1106 46-47 0.5060222733432695 1106 48-49 0.7876415953225759 1106 50-51 0.8151198991453938 1106 52-53 0.7920106321911078 1106 54-55 0.27736851453484945 1106 56-57 0.5366659901757416 1106 58-59 0.5052799669515124 1106 60-61 0.8691476318574729 1106 62-63 0.7169538784845528 1106 64-65 0.3450302455346943 1106 66-67 0.9724962201034373 1106 68-69 0.8009976356369819 1106 70-71 0.5828412097421278 1106 72-73 0.6087688694951403 1106 74-75 0.4931608586104126 1106 76-77 0.03123665805643938 1106 78-79 0.25962924063300363 1106 80-81 0.4627695408866934 1106 82-83 0.2635929521595024 1106 84-85 0.5923177644216508 1106 86-87 0.7089608142131176 1106 88-89 0.3749607129181207 1106 90-91 0.18407435685928064 1106 92-93 0.1659208376469934 1106 94-95 0.20445970012486114 1106 96-97 0.6339082542426091 1106 98-99 0.21652526570969144 1106 100-101 -0.37216012146505406 1107 1 -0.6615047717116873 1107 2 -0.5813379268244177 1107 3 -0.5961371682173855 1107 4 -0.8405767795520092 1107 5 -1.2031591323748643 1107 6 -0.9321124085392753 1107 7 -1.1192798174770502 1107 8 -0.6319138184902897 1107 9 -1.180680932572777 1107 10-11 -0.6751544342396159 1107 12-13 -0.7621747329413324 1107 14-15 -0.9010314732487288 1107 16-17 -0.9727821951936662 1107 18-19 -0.7824230462317558 1107 20-21 -0.6263877256118491 1107 22-23 -0.7702778831390305 1107 24-25 -0.8593370733351193 1107 26-27 -0.7849279748137761 1107 28-29 -0.4853862416825123 1107 30-31 -0.5065126663659498 1107 32-33 -0.602003503387067 1107 34-35 -1.1022877381394096 1107 36-37 -0.6551375032768405 1107 38-39 -0.905624608229239 1107 40-41 -0.7565461080710705 1107 42-43 -1.3660764368064306 1107 44-45 -1.1201415891627562 1107 46-47 -1.0711999488789559 1107 48-49 -1.1690250713441017 1107 50-51 -1.2776578786323753 1107 52-53 -1.3850095698290872 1107 54-55 -1.1582880511217155 1107 56-57 -1.3157329997232452 1107 58-59 -0.9523715481999986 1107 60-61 -0.836862469152635 1107 62-63 -0.6117329902023059 1107 64-65 -0.9193636938592462 1107 66-67 -1.1850542849470713 1107 68-69 -0.9710478189084739 1107 70-71 -1.0749365680356533 1107 72-73 -1.164589716363448 1107 74-75 -1.169768434318879 1107 76-77 -1.1974754631556834 1107 78-79 -1.5071131836094196 1107 80-81 -1.0896799540628024 1107 82-83 -0.847644421577872 1107 84-85 -0.9209903163864297 1107 86-87 -1.3044735292212266 1107 88-89 -1.0637514082939994 1107 90-91 -0.8865064512215319 1107 92-93 -1.293675121948965 1107 94-95 -1.0647322190670572 1107 96-97 -1.6908392205048663 1107 98-99 -1.5053181686337425 1107 100-101 -1.4427156770206118 1108 1 0.36659046638355264 1108 2 0.6589001684136768 1108 3 0.6667199746397579 1108 4 0.30037560140037556 1108 5 0.4666027723870414 1108 6 0.4900304486035836 1108 7 0.3521487539515249 1108 8 0.5009433243668582 1108 9 0.655033353141512 1108 10-11 0.5669884229032363 1108 12-13 0.41687288610629025 1108 14-15 0.45563519341794034 1108 16-17 0.5700749476634783 1108 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-1.0150272100777897 2316 46-47 -0.8207751122776443 2316 48-49 -0.8022276857231816 2316 50-51 -1.0688670289591684 2316 52-53 -1.138767739763729 2316 54-55 -1.2964579857622383 2316 56-57 -1.3748016271742287 2316 58-59 -1.05725716911504 2316 60-61 -0.9753265214402091 2316 62-63 -0.875589199352639 2316 64-65 -1.1254094454932257 2316 66-67 -1.2024889254699431 2316 68-69 -1.1486458581241585 2316 70-71 -0.8975509471206173 2316 72-73 -0.7581354679974339 2316 74-75 -0.7556507872600591 2316 76-77 -1.0523937638092775 2316 78-79 -1.180953052890466 2316 80-81 -0.6122453135399262 2316 82-83 -0.9385757941268906 2316 84-85 -0.9035229961249911 2316 86-87 -0.9351271239924657 2316 88-89 -0.6335063102547842 2316 90-91 -1.0259672355352514 2316 92-93 -0.8655705467855697 2316 94-95 -0.82584333017817 2316 96-97 -1.102277129001596 2316 98-99 -0.6410207830128272 2316 100-101 -0.37216012146505406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2250.0 3 238.0 4 181.0 5 168.0 6 192.0 7 214.0 8 261.0 9 347.0 10 430.0 11 580.0 12 1076.0 13 1664.0 14 1784.0 15 1935.0 16 2468.0 17 3505.0 18 2989.0 19 1518.0 20 1249.0 21 1347.0 22 1335.0 23 1310.0 24 1421.0 25 1548.0 26 1742.0 27 2062.0 28 2429.0 29 2887.0 30 3451.0 31 4261.0 32 5472.0 33 7175.0 34 9959.0 35 17241.0 36 23422.0 37 40232.0 38 57140.0 39 14428.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22858431018936 22.5 14.478809738503157 19.792605951307486 2 14.498122833822343 24.902085389652825 39.49260175682923 21.107190019695597 3 18.15145820094374 31.44044383751797 31.15786247707125 19.250235484467037 4 10.750982442225187 19.51364603237553 41.23868839456322 28.496683130836065 5 10.084321439999279 41.914200343414954 35.50739333630779 12.494084880277976 6 24.7343421870916 41.835134428711775 18.43922091734189 14.991302466854739 7 23.21551696943204 37.40395405116742 24.215972131465215 15.164556847935323 8 23.373773062813775 35.16318602576005 22.78159842086762 18.681442490558556 9 26.34159752299656 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17.931956049502897 34-35 17.586298045966885 30.39878180754276 33.520625905115644 18.494294241374714 36-37 18.75216520055701 29.68356938264896 33.04622490914649 18.518040507647545 38-39 18.78656559618903 29.650509880635408 33.7621583437483 17.800766179427267 40-41 17.18763799908963 30.35379852755679 34.74304711048306 17.715516362870517 42-43 17.733305254958438 30.85632250277953 32.87683330276413 18.533538939497895 44-45 17.43090800794476 31.407839220562156 32.24670421629309 18.91454855519999 46-47 17.482283076686137 30.756442980836958 31.968373395058514 19.79290054741839 48-49 18.318727788737913 30.81637784004041 31.67504343158195 19.189850939639726 50-51 18.84999875408603 30.931739771612644 31.200857188810332 19.01740428549099 52-53 18.137154972071595 31.04628459339594 31.95005051122799 18.866509923304477 54-55 18.089354021202723 30.65268250039067 31.797747465162256 19.460216013244345 56-57 18.70695843778803 31.55562198383528 30.155872448644523 19.581547129732165 58-59 17.805793274620058 30.25968055690802 31.464668996175106 20.469857172296816 60-61 18.36170410309946 31.16082003111462 30.556190881648938 19.921284984136978 62-63 19.245018115942027 30.46580615942029 31.107789855072465 19.18138586956522 64-65 18.85966004959969 30.04925996806595 31.625125980952813 19.465954001381544 66-67 18.157776162626146 30.56848054232867 31.401944068959427 19.871799226085756 68-69 18.099502419955744 30.671948217250193 31.469163399669792 19.759385963124267 70-71 18.846250517978085 30.49515308375281 30.86492716605415 19.793669232214953 72-73 18.70138163898935 29.830358012305897 30.109584511874488 21.358675836830272 74-75 18.86229647060955 30.52357645502705 30.319509151320094 20.294617923043297 76-77 18.9110969197114 29.61488466468289 31.862032419799718 19.611985995805988 78-79 18.129752840947692 29.559217171145303 32.231678208606404 20.0793517793006 80-81 18.720856235038873 31.3053009663444 30.47506460036099 19.498778198255735 82-83 18.786263310701553 30.06531359129635 30.571471276966765 20.576951821035326 84-85 18.265054238286115 29.532350476708107 30.636592159793462 21.566003125212312 86-87 19.41302097841916 29.579050116748085 30.581203898095115 20.426725006737644 88-89 20.08960727730689 28.368313996946647 31.15959427564431 20.382484450102154 90-91 19.011704041360623 28.479607418450065 31.917188130117403 20.591500410071912 92-93 19.27978868485318 29.957456062850852 30.86496943995143 19.897785812344537 94-95 18.805979613512786 29.05176337905686 31.007751937984494 21.13450506944586 96-97 19.233172445643806 28.48730858741816 30.04487045975242 22.23464850718561 98-99 19.394606145390505 28.832267148374015 30.148360429371472 21.624766276864012 100-101 19.93357437338266 28.551020269551298 30.45594604446034 21.0594593126057 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1831.0 1 1057.5 2 431.5 3 575.5 4 403.0 5 320.0 6 623.5 7 992.5 8 1238.0 9 1170.5 10 799.5 11 558.0 12 530.0 13 557.5 14 556.0 15 493.5 16 501.0 17 551.0 18 539.5 19 542.5 20 586.5 21 687.5 22 826.5 23 934.0 24 1044.5 25 1299.5 26 1854.0 27 2504.5 28 2876.5 29 3439.0 30 4571.0 31 5557.0 32 6613.0 33 7996.0 34 9022.5 35 10049.0 36 11708.0 37 12393.5 38 11257.5 39 9925.0 40 8812.5 41 7737.5 42 6338.5 43 6066.0 44 6303.0 45 5757.0 46 5219.5 47 5352.0 48 10446.5 49 10850.5 50 6530.5 51 4897.5 52 2895.5 53 2063.0 54 1594.0 55 1382.5 56 1368.5 57 1320.0 58 1226.0 59 1102.0 60 1025.0 61 980.0 62 819.0 63 626.5 64 441.0 65 305.5 66 276.0 67 292.5 68 288.0 69 235.5 70 173.0 71 147.0 72 125.0 73 112.5 74 102.5 75 87.0 76 51.5 77 24.0 78 11.5 79 4.0 80 3.0 81 2.0 82 1.5 83 2.5 84 2.0 85 1.5 86 1.0 87 0.5 88 0.5 89 0.0 90 1.0 91 1.5 92 1.0 93 1.0 94 2.0 95 2.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05182164342203096 2 0.017123673478584144 3 0.014419935560912962 4 0.008561836739292072 5 0.011716197643241782 6 0.005858098821620891 7 0.007209967780456481 8 0.009463082711849131 9 0.015321181533470021 10-11 0.0029290494108104455 12-13 0.009463082711849131 14-15 0.007435279273595746 16-17 0.018475542437419733 18-19 0.013969312574634433 20-21 0.0038302953833675053 22-23 0.010139017191266928 24-25 0.019376788409976795 26-27 0.008561836739292072 28-29 0.020052722889394588 30-31 0.016222427506027082 32-33 0.013068066602077374 34-35 0.27195097221909287 36-37 0.49230561250929405 38-39 0.4862222021945339 40-41 0.5071761710564856 42-43 0.49771308834463646 44-45 0.5139355158506635 46-47 0.5188923686997274 48-49 0.5252010905076268 50-51 0.5378185341234256 52-53 0.5281301399184373 54-55 0.5143861388369421 56-57 0.5071761710564856 58-59 0.5067255480702071 60-61 0.5040218101525359 62-63 0.502444629700561 64-65 0.5170898767546133 66-67 0.4909537435504585 68-69 0.5166392537683347 70-71 0.49771308834463646 72-73 0.5076267940427641 74-75 0.520244237658563 76-77 0.5065002365770679 78-79 0.5074014825496248 80-81 0.5112317779329923 82-83 0.5110064664398531 84-85 0.509203974494739 86-87 0.5139355158506635 88-89 0.5290313858909943 90-91 0.5506612892323638 92-93 0.5416488295067932 94-95 0.5098799089741568 96-97 0.4763084964964063 98-99 0.46594416781200004 100-101 0.4805894148660523 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 221915.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.603496834373523 #Duplication Level Percentage of deduplicated Percentage of total 1 69.17526136083636 17.711285852691347 2 10.169312541800133 5.207399229434693 3 4.85937555000176 3.7325101953450646 4 3.0008096025907283 3.073248766419575 5 2.027526488084762 2.5955884009643335 6 1.4449646238867964 2.219768830408039 7 1.09472350311521 1.9620124822567198 8 0.9292829737055159 1.903431494040511 9 0.7356823541835333 1.6952436743798303 >10 5.580977859129149 28.61050402181015 >50 0.6828821852229927 11.892391230876687 >100 0.2692808616987574 11.530540972895027 >500 0.017600056320180225 2.7217628371223217 >1k 0.01232003942412616 5.144312011355699 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2109 0.9503638780614199 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2026 0.9129621702003019 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1779 0.8016582925895049 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1686 0.7597503548656017 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1595 0.7187436631142554 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1122 0.5055989906045107 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1099 0.4952346619201045 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 743 0.3348128788049478 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 671 0.3023680237928937 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 662 0.2983124169163869 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 619 0.2789356285064101 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 605 0.2726269066985107 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 580 0.26136133204154743 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 573 0.2582069711375977 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 535 0.2410832976590136 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 532 0.23973142870017797 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 520 0.23432395286483565 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 423 0.19061352319581823 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 410 0.18475542437419734 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 385 0.17348984971723408 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 374 0.16853299686817025 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 372 0.1676317508956132 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 349 0.157267422211207 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 336 0.1514093233895861 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 329 0.1482549624856364 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 327 0.14735371651307935 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 326 0.1469030935268008 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 323 0.1455512245679652 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 310 0.13969312574634432 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 301 0.13563751886983755 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 300 0.135186895883559 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 280 0.12617443615798843 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 271 0.12211882928148164 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 270 0.12166820629520311 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 269 0.12121758330892458 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 264 0.11896446837753194 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 263 0.11851384539125341 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 255 0.11490886150102517 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 252 0.11355699254218957 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 251 0.11310636955591104 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 238 0.10724827073429015 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 224 0.10093954892639073 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 223 0.1004889259401122 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 223 0.1004889259401122 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2531149313926503E-4 0.0 14-15 0.0 0.0 0.0 0.0013518689588355901 0.0 16-17 0.0 0.0 0.0 0.0018024919451141202 0.0 18-19 0.0 0.0 0.0 0.0027037379176711803 0.0 20-21 0.0 0.0 0.0 0.004055606876506771 0.0 22-23 0.0 0.0 0.0 0.009913705698127662 0.0 24-25 0.0 0.0 0.0 0.0180249194511412 0.0 26-27 0.0 0.0 0.0 0.03086767456007931 0.0 28-29 0.0 0.0 0.0 0.06151003762701936 0.0 30-31 0.0 0.0 0.0 0.1358628303629768 0.0 32-33 0.0 0.0 0.0 0.25617916769934435 0.0 34-35 0.0 0.0 0.0 0.44025865759412386 0.0 36-37 0.0 0.0 0.0 0.684271004663948 0.0 38-39 0.0 0.0 0.0 0.9783025032106888 0.0 40-41 0.0 0.0 0.0 1.3327174819187526 0.0 42-43 0.0 0.0 0.0 1.6440979654372168 0.0 44-45 0.0 0.0 0.0 1.9291170042583872 0.0 46-47 0.0 0.0 0.0 2.314850280512809 0.0 48-49 0.0 0.0 0.0 2.714552869341865 0.0 50-51 0.0 0.0 0.0 3.0721222089538784 0.0 52-53 0.0 0.0 0.0 3.3814748890340898 0.0 54-55 0.0 0.0 0.0 3.6815897978955903 0.0 56-57 0.0 0.0 0.0 3.9895906090169664 0.0 58-59 0.0 0.0 0.0 4.32237568438366 0.0 60-61 0.0 0.0 0.0 4.7038280422684355 0.0 62-63 0.0 0.0 0.0 5.04292183944303 0.0 64-65 0.0 0.0 0.0 5.391704030822613 0.0 66-67 0.0 0.0 0.0 5.7873510127751615 0.0 68-69 0.0 0.0 0.0 6.211837865849537 0.0 70-71 0.0 0.0 0.0 6.684766689948854 0.0 72-73 0.0 0.0 0.0 7.153865218664804 0.0 74-75 0.0 0.0 0.0 7.5943491877520675 0.0 76-77 0.0 0.0 0.0 8.084401685329969 0.0 78-79 0.0 0.0 0.0 8.643399499808485 0.0 80-81 0.0 0.0 0.0 9.243178694545207 0.0 82-83 0.0 2.2531149313926503E-4 0.0 9.850618480048666 0.0 84-85 0.0 4.5062298627853006E-4 0.0 10.505373679111372 0.0 86-87 0.0 4.5062298627853006E-4 0.0 11.195052159610661 0.0 88-89 0.0 4.5062298627853006E-4 0.0 11.830655881756527 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATGC 25 5.34875E-5 75.76112 3 ATGCTCA 25 0.0050879116 56.79522 9 GACATAG 35 2.826633E-4 54.115086 1 GACAGAT 35 2.8329378E-4 54.09069 7 CAGATAG 35 2.8329378E-4 54.09069 9 ATAGACA 40 5.4689246E-4 47.340023 4 GGGTCCT 70 8.108691E-8 47.329353 7 GGTCCTA 75 1.3950557E-7 44.174057 8 ATAAGCC 55 5.043124E-5 43.04609 3 ATATGCT 55 5.0498427E-5 43.036385 4 ATATAAG 70 4.3883483E-6 40.586315 1 GTATAGT 140 0.0 40.586315 1 AGGGGTC 95 1.9741492E-8 39.86528 5 GCTCCGT 30 8.215731E-5 39.637707 50-51 AAGCCTC 60 8.43512E-5 39.45002 5 GTATAGG 145 0.0 39.186783 1 GGATGCC 85 3.7267273E-7 38.97711 8 GCCTCCG 25 0.0016038852 38.0522 60-61 GGCGGTT 25 0.0016038852 38.0522 46-47 ACTATAT 50 0.001641777 37.86348 8 >>END_MODULE