##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936349_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 221915 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31972151499448 33.0 31.0 34.0 30.0 34.0 2 32.441826825586375 34.0 31.0 34.0 31.0 34.0 3 32.505788252258746 34.0 31.0 34.0 30.0 34.0 4 36.03774418133069 37.0 35.0 37.0 35.0 37.0 5 36.025518779712954 37.0 35.0 37.0 35.0 37.0 6 36.10118739156884 37.0 35.0 37.0 35.0 37.0 7 36.05324110582881 37.0 35.0 37.0 35.0 37.0 8 36.05357907306852 37.0 35.0 37.0 35.0 37.0 9 37.76131852285785 39.0 38.0 39.0 35.0 39.0 10-11 37.72755559561093 39.0 37.5 39.0 35.0 39.0 12-13 37.6923709528423 39.0 37.0 39.0 35.0 39.0 14-15 38.856267039181674 40.0 38.0 41.0 34.5 41.0 16-17 38.79680733614222 40.0 38.0 41.0 34.5 41.0 18-19 38.94625419642656 40.0 38.0 41.0 35.0 41.0 20-21 38.937593222630284 40.0 38.0 41.0 35.0 41.0 22-23 38.8100196020999 40.0 38.0 41.0 35.0 41.0 24-25 38.90987540274429 40.0 38.0 41.0 35.0 41.0 26-27 38.73571187166257 40.0 38.0 41.0 35.0 41.0 28-29 38.58102426604781 40.0 38.0 41.0 34.5 41.0 30-31 38.38120000901246 40.0 37.5 41.0 34.0 41.0 32-33 38.077306626411016 40.0 37.0 41.0 34.0 41.0 34-35 37.88452785976612 40.0 36.0 41.0 33.0 41.0 36-37 37.66896108870513 40.0 36.0 41.0 33.0 41.0 38-39 37.42640650699592 40.0 36.0 41.0 33.0 41.0 40-41 37.23595520807517 40.0 35.5 41.0 33.0 41.0 42-43 37.103825789153504 40.0 36.0 41.0 33.0 41.0 44-45 36.78672689993917 40.0 36.0 41.0 31.5 41.0 46-47 36.32625780141045 40.0 35.0 41.0 30.5 41.0 48-49 35.90860013969313 40.0 35.0 41.0 29.0 41.0 50-51 35.64601536624383 40.0 35.0 41.0 27.0 41.0 52-53 35.40647995854269 40.0 35.0 41.0 26.0 41.0 54-55 35.245835117049324 40.0 35.0 41.0 26.0 41.0 56-57 35.040031092986055 39.0 35.0 41.0 24.0 41.0 58-59 34.81074059887795 39.0 35.0 41.0 23.0 41.0 60-61 34.54781109884415 39.0 35.0 41.0 22.0 41.0 62-63 34.240114458238516 38.5 35.0 40.5 20.5 41.0 64-65 33.84824144379605 38.0 34.0 40.0 17.5 41.0 66-67 33.47723002050334 37.5 34.0 40.0 11.5 41.0 68-69 33.09751256111574 37.0 34.0 39.5 7.5 41.0 70-71 32.7114908861501 36.5 33.5 39.0 2.0 41.0 72-73 32.265563391388596 36.0 33.0 39.0 2.0 40.5 74-75 31.823164274609645 35.0 33.0 38.0 2.0 40.0 76-77 31.02424576977672 35.0 31.5 37.0 2.0 39.0 78-79 31.010055651938806 35.0 32.0 37.0 2.0 39.0 80-81 30.83988013428565 35.0 32.0 36.0 2.0 39.0 82-83 30.539098303404458 35.0 32.0 36.0 2.0 37.5 84-85 30.204731541355926 35.0 32.0 35.5 2.0 37.0 86-87 29.80906202825406 35.0 31.0 35.0 2.0 37.0 88-89 29.258493116733884 34.5 30.5 35.0 2.0 36.0 90-91 28.992107338395332 34.0 30.0 35.0 2.0 36.0 92-93 28.747288376180066 34.0 30.0 35.0 2.0 36.0 94-95 28.624579230786562 34.0 30.0 35.0 2.0 35.0 96-97 28.393558344411147 34.0 30.0 35.0 2.0 35.0 98-99 28.0307685375031 34.0 29.5 35.0 2.0 35.0 100-101 25.19795642475723 30.5 23.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19829917045959178 1101 2 0.07948014943746529 1101 3 0.03880303004733321 1101 4 0.2139563876692634 1101 5 0.03892229646246648 1101 6 0.13684580374079758 1101 7 0.3234490071872287 1101 8 0.10427861995484733 1101 9 0.34844934512288006 1101 10-11 0.21596907891987627 1101 12-13 0.315088907749427 1101 14-15 0.46665875528580614 1101 16-17 0.2730933871225858 1101 18-19 0.3603142653689986 1101 20-21 0.183628252981606 1101 22-23 0.25437031690999135 1101 24-25 0.2836002307562495 1101 26-27 0.1882805086544579 1101 28-29 0.30779480331283793 1101 30-31 -0.17190948088261138 1101 32-33 0.2630387534696297 1101 34-35 0.36369688558999513 1101 36-37 0.43807299073922223 1101 38-39 0.3906638755604135 1101 40-41 0.41208342877623494 1101 42-43 0.47441880186822516 1101 44-45 0.1433274045372599 1101 46-47 0.715525623589393 1101 48-49 0.5085687249615845 1101 50-51 0.31507149274006707 1101 52-53 0.5836799583394523 1101 54-55 0.7519114042843924 1101 56-57 0.7178098963087791 1101 58-59 1.0812570761054943 1101 60-61 0.6560379817115347 1101 62-63 0.5917699243752139 1101 64-65 0.876548007373728 1101 66-67 0.8101669933036746 1101 68-69 1.012272731746556 1101 70-71 1.348828725971508 1101 72-73 0.6522269905788534 1101 74-75 1.0573378636641877 1101 76-77 0.9055850539014578 1101 78-79 0.9188532077465972 1101 80-81 0.8883638766597777 1101 82-83 1.0726607189634905 1101 84-85 1.3289186832297766 1101 86-87 1.2789568553906605 1101 88-89 1.4001686368594122 1101 90-91 1.5068880530241415 1101 92-93 1.6709050482830747 1101 94-95 1.826252949664994 1101 96-97 1.3479374528284218 1101 98-99 1.322634063748307 1101 100-101 1.7478567761149861 1102 1 0.13478268694310458 1102 2 0.23904058899790215 1102 3 0.1816601729044791 1102 4 0.24208825580112858 1102 5 0.15101020855038172 1102 6 0.23904360593860474 1102 7 0.3001523038905276 1102 8 -0.05967742400120102 1102 9 0.11460319127672847 1102 10-11 -0.007327624376834763 1102 12-13 0.2598141824746989 1102 14-15 0.3507246893517433 1102 16-17 0.22166481569401952 1102 18-19 0.25196261701735523 1102 20-21 0.32758429693765123 1102 22-23 0.2927219652616415 1102 24-25 0.13876506592107773 1102 26-27 0.22751127788522751 1102 28-29 0.2732893088073425 1102 30-31 0.42083777186464744 1102 32-33 0.3609508413817153 1102 34-35 0.7825979844910975 1102 36-37 0.6916993643655971 1102 38-39 1.1035210184175597 1102 40-41 1.1008746375674434 1102 42-43 0.8160671535165847 1102 44-45 1.2406900418998958 1102 46-47 0.6235476016113708 1102 48-49 0.8561511425440003 1102 50-51 0.8271594048279738 1102 52-53 0.9074162220757174 1102 54-55 1.0625707449437272 1102 56-57 0.9853923138911966 1102 58-59 1.0966416914901131 1102 60-61 0.7034006190741664 1102 62-63 1.3292424518477475 1102 64-65 0.8011633919891139 1102 66-67 1.1099472130838919 1102 68-69 1.1101848196586488 1102 70-71 0.8628946600374334 1102 72-73 0.6570621554140175 1102 74-75 0.3664587427850634 1102 76-77 0.2917389000553001 1102 78-79 0.5100619989553863 1102 80-81 0.21913310742900904 1102 82-83 -0.09228433598156371 1102 84-85 0.1682593425704333 1102 86-87 -0.05225193581813059 1102 88-89 -0.007084110393336118 1102 90-91 0.14238255851864778 1102 92-93 -0.1909630835850571 1102 94-95 -0.2151756217635814 1102 96-97 -0.3206339757430037 1102 98-99 0.04461208572632458 1102 100-101 1.364669962928172 1103 1 -0.023312551152130823 1103 2 -0.08512607766876101 1103 3 0.04029112528542811 1103 4 0.1631894462773218 1103 5 0.10205187521704318 1103 6 -0.002176632156633218 1103 7 -0.07850841039518741 1103 8 0.10496543314165763 1103 9 0.19981152461006246 1103 10-11 0.10716642324221937 1103 12-13 0.2293231110461278 1103 14-15 0.34752528458983534 1103 16-17 0.4140904109321184 1103 18-19 0.18517690273164078 1103 20-21 0.2034027493185988 1103 22-23 0.37928446526164095 1103 24-25 -0.02818433884081628 1103 26-27 -0.0815512221147685 1103 28-29 -0.04757378643075327 1103 30-31 0.2501086051979726 1103 32-33 0.2824984604293377 1103 34-35 0.34503846068157173 1103 36-37 0.22836603103227304 1103 38-39 -0.1338896958681559 1103 40-41 -0.18678905290875036 1103 42-43 0.16126060589753166 1103 44-45 0.1363448038046542 1103 46-47 0.08752081589708638 1103 48-49 0.43173149968685465 1103 50-51 0.26473380958988457 1103 52-53 0.0564936030281018 1103 54-55 -0.0372655645800819 1103 56-57 0.3359131472245238 1103 58-59 0.29187978672820947 1103 60-61 0.3250226428836882 1103 62-63 0.11961447565726502 1103 64-65 0.27195208246529745 1103 66-67 0.02622697498865989 1103 68-69 -0.3060651803413563 1103 70-71 -0.0020755780578056715 1103 72-73 -0.21121165410979614 1103 74-75 -0.07246982864350571 1103 76-77 -0.23040395708755668 1103 78-79 -0.16158978675889912 1103 80-81 -0.07097105923765668 1103 82-83 0.03557280687557807 1103 84-85 0.13074446161805398 1103 86-87 0.1149802070390109 1103 88-89 0.08520755627332832 1103 90-91 0.6448230347091268 1103 92-93 1.0014625116530347 1103 94-95 0.7369672353792787 1103 96-97 0.7497231671141371 1103 98-99 0.7024989904882304 1103 100-101 0.6403991295948366 1104 1 0.4141874488478692 1104 2 0.5815405889979033 1104 3 0.6340411252854281 1104 4 0.5329811129439861 1104 5 0.4249685418837146 1104 6 0.3467817011766954 1104 7 0.4683665896048126 1104 8 0.16225709980832193 1104 9 0.38731152461006246 1104 10-11 0.6253955899088837 1104 12-13 0.2866147777127921 1104 14-15 0.915233617923171 1104 16-17 0.8984654109321184 1104 18-19 0.8258019027316408 1104 20-21 0.7606944159852631 1104 22-23 0.6423052985949695 1104 24-25 0.7348364944925123 1104 26-27 0.9601154445519029 1104 28-29 0.9862803802359181 1104 30-31 0.948025271864644 1104 32-33 0.9778109604293377 1104 34-35 1.1705592940149074 1104 36-37 1.267428531032273 1104 38-39 1.3530894707985155 1104 40-41 1.393940113757914 1104 42-43 1.1664689392308674 1104 44-45 1.352490637137997 1104 46-47 1.2151249825637578 1104 48-49 0.887460666353526 1104 50-51 0.7543171429232203 1104 52-53 0.8898269363614304 1104 54-55 0.8038802687532609 1104 56-57 0.8697673138911952 1104 58-59 1.1382339533948667 1104 60-61 1.3979393095503525 1104 62-63 1.6586769756572721 1104 64-65 1.6078895824652975 1104 66-67 1.6772686416553242 1104 68-69 1.407476486325315 1104 70-71 1.6619869219422014 1104 72-73 1.4658716792235467 1104 74-75 1.487426004689823 1104 76-77 1.4675127095791076 1104 78-79 1.3774727132411009 1104 80-81 1.613924774095679 1104 82-83 1.5147394735422424 1104 84-85 1.3911611282847183 1104 86-87 1.4300843737056788 1104 88-89 1.8690617229399997 1104 90-91 1.9495105347091268 1104 92-93 1.902504178319706 1104 94-95 1.927071402045943 1104 96-97 1.9502440004474728 1104 98-99 1.988957323821566 1104 100-101 1.3409199629281723 1105 1 0.049604115514533476 1105 2 0.008623922331238987 1105 3 0.1757077919520995 1105 4 0.1579811129439861 1105 5 0.01871854188371458 1105 6 0.06032336784336678 1105 7 0.10378325627147689 1105 8 0.05809043314165763 1105 9 0.27272819127672676 1105 10-11 0.26862475657554796 1105 12-13 0.08088561104612779 1105 14-15 0.35273361792317104 1105 16-17 0.3541945775987756 1105 18-19 0.04715606939830508 1105 20-21 0.1799652493186059 1105 22-23 0.08761779859497665 1105 24-25 0.3129614944925123 1105 26-27 0.2179279445519029 1105 28-29 0.37690538023591813 1105 30-31 0.17979610519797973 1105 32-33 0.4439567937626734 1105 34-35 0.15493429401491454 1105 36-37 0.6372201976989302 1105 38-39 0.3218394707985155 1105 40-41 0.33144011375791393 1105 42-43 0.38521893923086736 1105 44-45 0.414990637137997 1105 46-47 0.5744999825637578 1105 48-49 0.16871066635351895 1105 50-51 -8.911904101225332E-4 1105 52-53 -0.0112147303052339 1105 54-55 0.2804427687532609 1105 56-57 -0.1250243527754762 1105 58-59 -0.0075993799384619365 1105 60-61 -0.06039402378297609 1105 62-63 0.22378114232392932 1105 64-65 0.10007708246530456 1105 66-67 0.1564353083219885 1105 68-69 0.3918514863253151 1105 70-71 0.63594525527553 1105 72-73 0.39295501255687526 1105 74-75 0.4301343380231657 1105 76-77 0.3893877095791076 1105 78-79 0.24205604657443658 1105 80-81 0.16340394076234332 1105 82-83 0.39494780687557807 1105 84-85 0.599494461618054 1105 86-87 0.7269593737056788 1105 88-89 0.4836450562733319 1105 90-91 0.860968868042459 1105 92-93 0.7228166783197025 1105 94-95 0.6458214020459465 1105 96-97 0.6846190004474728 1105 98-99 0.6842698238215625 1105 100-101 1.1143574629281723 1106 1 -0.07886810670768796 1106 2 0.03924260919992406 1106 3 -0.2598982686539628 1106 4 -0.08097090725803469 1106 5 -0.04393928639911593 1106 6 -0.19804153114653644 1106 7 0.027078710816937246 1106 8 -0.08253456685834237 1106 9 0.017835514509052075 1106 10-11 -0.24526413231334487 1106 12-13 -0.29474570208518003 1106 14-15 -0.42070388207682896 1106 16-17 -0.22834961432040757 1106 18-19 -0.37606047100572937 1106 20-21 -0.35538828603493045 1106 22-23 -0.35485379231411684 1106 24-25 -0.23296653581051885 1106 26-27 -0.15359983322588278 1106 28-29 -0.27942353390549357 1106 30-31 -0.2373440463171761 1106 32-33 -0.15626416583329217 1106 34-35 0.014546036439149646 1106 36-37 -0.08729053462429448 1106 38-39 -0.05702732213078576 1106 40-41 -0.546874280181477 1106 42-43 -0.716501389051956 1106 44-45 -0.2124557012458439 1106 46-47 -0.32448991642615255 1106 48-49 -0.3404433740505155 1106 50-51 -0.2266645489959771 1106 52-53 -0.014055639396147512 1106 54-55 -0.10836720599422023 1106 56-57 -0.7231935446946594 1106 58-59 -0.4858979910495762 1106 60-61 -0.412587836914291 1106 62-63 -0.39482681222152394 1106 64-65 -0.45745132662560906 1106 66-67 -0.3849157017790148 1106 68-69 -0.14728992781610373 1106 70-71 -0.2683318911891135 1106 72-73 -0.4318555935037338 1106 74-75 -0.19305063672431544 1106 76-77 0.058737457053855024 1106 78-79 -0.2599957211023316 1106 80-81 -0.3505638622679612 1106 82-83 -0.18033628403351543 1106 84-85 -0.2512788969678077 1106 86-87 -0.17242509599129363 1106 88-89 -0.22082148413071323 1106 90-91 0.0946336407697288 1106 92-93 0.06861844599647071 1106 94-95 0.18014905356109878 1106 96-97 -0.13222127733030575 1106 98-99 -0.020828029713786833 1106 100-101 0.3412356194938262 1107 1 -0.47164588448546496 1107 2 -0.24595941100209728 1107 3 -0.4176255413812342 1107 4 -0.16076888705601533 1107 5 -0.21898979144962283 1107 6 -0.2373849654899658 1107 7 -0.24913341039518855 1107 8 -0.017534566858344647 1107 9 -0.28039680872327466 1107 10-11 -0.37304191009111776 1107 12-13 -0.26161438895387334 1107 14-15 -0.4507038820768301 1107 16-17 -0.327263755734549 1107 18-19 -0.27232309726835524 1107 20-21 -0.32420141734806407 1107 22-23 -0.36477803473835735 1107 24-25 -0.2401635055074891 1107 26-27 -0.26998872211476765 1107 28-29 -0.6677821197640839 1107 30-31 -0.38166222813535455 1107 32-33 -0.5883348729039923 1107 34-35 -0.7620448726517566 1107 36-37 -0.9558006356343967 1107 38-39 -0.7707646958681593 1107 40-41 -1.0987682195754118 1107 42-43 -0.8107185607691321 1107 44-45 -0.8957385295286713 1107 46-47 -1.1996666841029153 1107 48-49 -1.5092060003131493 1107 50-51 -1.1764120237434526 1107 52-53 -0.7107980636385705 1107 54-55 -0.8877863979134162 1107 56-57 -1.0346076861088065 1107 58-59 -0.8622868799384591 1107 60-61 -0.5369565237829761 1107 62-63 -0.5436146910093953 1107 64-65 -0.8891937508680314 1107 66-67 -1.1464813583446798 1107 68-69 -1.0623151803413577 1107 70-71 -0.9085339113911353 1107 72-73 -0.9332949874431264 1107 74-75 -1.1049698286435046 1107 76-77 -1.340403957087556 1107 78-79 -1.2206522867588987 1107 80-81 -0.900866892570992 1107 82-83 -0.8844271931244236 1107 84-85 -1.0785263717152809 1107 86-87 -1.459394792960989 1107 88-89 -1.164584110393335 1107 90-91 -1.4622602986242086 1107 92-93 -1.1398916550136313 1107 94-95 -0.938032764620722 1107 96-97 -1.1152768328858649 1107 98-99 -1.2514593428451022 1107 100-101 -1.7928300370718269 1108 1 -0.2518839797235586 1108 2 0.13332630328361716 1108 3 0.05308874433305277 1108 4 0.02970730342017447 1108 5 -0.034704077163908664 1108 6 0.02475789165288944 1108 7 -0.06556198182375539 1108 8 -0.26348694781072624 1108 9 0.10412700080053838 1108 10-11 0.20791047086126468 1108 12-13 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-0.13135054299868187 2316 74-75 -0.10392407700951978 2316 76-77 -0.40605755185879744 2316 78-79 -0.3975477115955002 2316 80-81 -0.13250087949909783 2316 82-83 -0.3475971277649421 2316 84-85 -0.0887714697544979 2316 86-87 -0.4118784530917097 2316 88-89 -0.1755154829423553 2316 90-91 -0.3905119326111368 2316 92-93 -0.8657249883469618 2316 94-95 -0.6756144639671255 2316 96-97 -0.758038270794362 2316 98-99 -0.9761162055901984 2316 100-101 -0.9868986645228084 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 2.0 5 8.0 6 17.0 7 42.0 8 90.0 9 147.0 10 298.0 11 496.0 12 786.0 13 1254.0 14 1393.0 15 1914.0 16 2700.0 17 3681.0 18 3127.0 19 1586.0 20 1212.0 21 1301.0 22 1332.0 23 1394.0 24 1423.0 25 1573.0 26 1713.0 27 1932.0 28 2450.0 29 2847.0 30 3690.0 31 4514.0 32 5620.0 33 7228.0 34 9999.0 35 16464.0 36 22234.0 37 40918.0 38 61840.0 39 14685.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.368638649538894 22.733002887261257 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31.569294549714982 34.35324335894374 17.2662956537413 20-21 18.225487744153718 30.4779543472824 33.304493397231816 17.992064511332067 22-23 18.140319807853924 29.87141444114738 32.77771393937755 19.210551811621144 24-25 18.420548639666535 30.103757111474117 33.4307440995888 18.044950149270548 26-27 18.367949313012875 29.622911888099285 34.26576420276597 17.743374596121868 28-29 17.7638931449069 28.720686050074807 35.63548858085333 17.879932224164968 30-31 17.950607103186137 30.499584299358535 34.18208364032094 17.367724957134392 32-33 16.833173360731458 31.360938746265425 33.97750469778697 17.828383195216144 34-35 17.332732495916183 29.805216019827636 34.22114572184983 18.640905762406355 36-37 18.596534709235517 29.347723227361826 33.42090440033346 18.63483766306919 38-39 18.682829011107856 29.134353243358944 34.32823378320528 17.85458396232792 40-41 16.906060667509333 29.769348104788108 35.358888220463285 17.965703007239277 42-43 17.546500230952088 30.25697644288724 33.58831945743159 18.60820386872909 44-45 17.161996521049453 31.00592142618947 32.79158742891134 19.04049462384974 46-47 17.49558892126309 30.138202533299985 32.35399498387675 20.01221356156018 48-49 18.16057453314675 30.107330615703216 32.315010737568414 19.417084113581616 50-51 18.5590100178907 30.497334420895616 31.623276837176604 19.32037872403708 52-53 17.916467629854623 30.62770952943191 32.51525448170814 18.940568359005326 54-55 17.62033941076273 30.20471894517696 32.1980587074272 19.97688293663311 56-57 18.57591556975544 31.27470021765483 30.193861503035002 19.95552270955473 58-59 17.458947690976604 30.174348390352932 31.80765348426403 20.55905043440643 60-61 18.226102630541316 31.225370359939163 30.447135695375426 20.101391314144088 62-63 18.832661154045468 30.683144447198252 30.944956402226076 19.539237996530204 64-65 18.451950439702316 30.188611225646262 31.326226896758392 20.033211437893033 66-67 17.83858684631503 30.503571187166255 31.150891106955363 20.506950859563347 68-69 17.81425320505599 30.6621904783363 31.241466327197347 20.28208998941036 70-71 18.713288225870777 30.31280065070106 30.660457067924806 20.313454055503357 72-73 18.599799018525445 29.65603327460108 29.758326536314105 21.985841170559368 74-75 18.546290246265464 30.165153324471085 30.01937678840998 21.26917964085348 76-77 18.51720572365183 29.594251062235006 31.324531420030976 20.564011794082187 78-79 17.873761946329765 29.412388318592853 31.670264381272034 21.043585353805344 80-81 18.22266183889451 31.09136341002341 29.84829469538395 20.83768005569813 82-83 18.22184169614492 29.89252641777257 30.179798571525136 21.705833314557378 84-85 17.8108735326589 29.289142239145622 29.95110740598878 22.948876822206703 86-87 18.773827946995368 29.38806448265438 30.21665666550864 21.621450904841613 88-89 19.40032275319353 28.228438545434763 30.70280414502135 21.66843455635035 90-91 18.075791205760964 28.337846329103623 31.554429441307697 22.031933023827712 92-93 18.31816630773507 29.56451390568046 30.49916726363055 21.61815252295392 94-95 17.847671450169777 28.66992476628881 30.66782029530189 22.814583488239524 96-97 18.31239790457951 28.273305920126173 29.61685348955106 23.797442685743256 98-99 18.421532908389153 28.58774781838706 29.732573570995648 23.25814570222814 100-101 18.48087404606812 28.419114913444837 30.256342083858133 22.843668956628914 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1761.0 1 1032.0 2 447.0 3 594.0 4 391.5 5 283.0 6 577.0 7 961.0 8 1193.0 9 1135.0 10 772.5 11 467.0 12 423.5 13 429.5 14 438.0 15 442.0 16 435.0 17 449.0 18 502.5 19 537.5 20 592.0 21 666.5 22 738.0 23 824.0 24 982.0 25 1265.0 26 1780.5 27 2384.5 28 2721.0 29 3292.0 30 4468.5 31 5556.0 32 6664.0 33 8013.0 34 9049.0 35 10038.0 36 11642.5 37 12389.5 38 11386.0 39 9969.5 40 8721.5 41 7702.0 42 6328.5 43 6183.0 44 6461.5 45 5835.0 46 5378.0 47 5434.5 48 10115.0 49 10127.5 50 5833.5 51 4964.5 52 3865.5 53 3221.5 54 2269.5 55 1528.5 56 1331.0 57 1313.5 58 1236.0 59 1070.0 60 1032.0 61 990.5 62 786.5 63 610.0 64 442.0 65 305.0 66 273.5 67 300.0 68 321.0 69 246.5 70 153.0 71 145.5 72 147.0 73 128.0 74 103.0 75 91.0 76 54.0 77 23.5 78 15.0 79 5.5 80 3.0 81 2.5 82 2.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.269472545794561 2 0.018926165423698262 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 4.5062298627853006E-4 14-15 0.0 16-17 4.5062298627853006E-4 18-19 0.0 20-21 2.2531149313926503E-4 22-23 2.2531149313926503E-4 24-25 0.001126557465696325 26-27 0.0018024919451141202 28-29 0.0031543609039497104 30-31 2.2531149313926503E-4 32-33 9.012459725570601E-4 34-35 0.001126557465696325 36-37 0.0 38-39 0.0 40-41 2.2531149313926503E-4 42-43 0.0033796723970889756 44-45 0.004055606876506771 46-47 0.013744001081495167 48-49 0.013744001081495167 50-51 0.0054074758353423605 52-53 0.005858098821620891 54-55 4.5062298627853006E-4 56-57 0.0018024919451141202 58-59 0.0013518689588355901 60-61 0.001126557465696325 62-63 0.0 64-65 0.0015771804519748552 66-67 0.0 68-69 0.0 70-71 2.2531149313926503E-4 72-73 0.0018024919451141202 74-75 0.0 76-77 0.20187909785278146 78-79 0.11107856611765765 80-81 0.0020278034382533853 82-83 0.0 84-85 0.0 86-87 0.12347069824031724 88-89 0.59166798098371 90-91 0.3163373363675281 92-93 0.295833990491855 94-95 0.0029290494108104455 96-97 0.001126557465696325 98-99 0.0015771804519748552 100-101 0.0020278034382533853 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 221915.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.996124642318005 #Duplication Level Percentage of deduplicated Percentage of total 1 66.82503614955587 16.035418966721494 2 11.073970441869637 5.3146475001689835 3 5.13793168203414 3.698713471374175 4 3.070364875776981 2.9470743302615867 5 2.2008976357251506 2.6406506995921863 6 1.5417550844115604 2.219768830408039 7 1.2243901522976095 2.056643309375211 8 0.9577284933616269 1.8385417840164027 9 0.820641865880453 1.7723002050334589 >10 6.088148579369401 29.098979338936083 >50 0.7305027135640645 12.151048825000563 >100 0.2967080430414452 12.178987450149831 >500 0.018778990065914256 2.8141405493094203 >1k 0.01314529304613998 5.233084739652569 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2222 1.0012842755108937 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1978 0.8913322668589324 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1822 0.8210350809994817 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1800 0.8111213753013541 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1535 0.6917062839375436 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1204 0.5425500754793502 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1052 0.47405538156501364 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 801 0.3609490120091026 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 698 0.314534844422414 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 677 0.3050717617105648 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 653 0.29425681003988013 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 630 0.2838924813554739 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 583 0.26271320100038303 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 580 0.26136133204154743 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 547 0.2464907734943559 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 542 0.24423765856296328 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 534 0.24063267467273503 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 443 0.19962598292138883 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 409 0.1843048013879188 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 397 0.17889732555257642 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 376 0.1694342428407273 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 362 0.16312552103282787 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 358 0.16132302908771376 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 351 0.15816866818376404 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 349 0.157267422211207 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 347 0.15636617623864993 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 332 0.14960683144447198 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 324 0.14600184755424375 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 322 0.1451006015816867 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 314 0.14149561769145844 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 310 0.13969312574634432 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 306 0.1378906338012302 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 303 0.1365387648423946 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 295 0.13293378095216637 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 293 0.13203253497960932 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 283 0.127526305116824 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 273 0.12302007525403871 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 265 0.11941509136381048 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 253 0.1140076155284681 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 250 0.11265574656963251 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 249 0.112205123583354 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 241 0.10860013969312575 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 227 0.10229141788522633 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 222 0.10003830295383367 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 222 0.10003830295383367 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.759344794177951E-4 0.0 14-15 0.0 0.0 0.0 0.00180249194511412 0.0 16-17 0.0 0.0 0.0 0.00225311493139265 0.0 18-19 0.0 0.0 0.0 0.0031543609039497104 0.0 20-21 0.0 0.0 0.0 0.0045062298627853 0.0 22-23 0.0 0.0 0.0 0.010589640177545457 0.0 24-25 0.0 0.0 0.0 0.018926165423698262 0.0 26-27 0.0 0.0 0.0 0.0322195435189149 0.0 28-29 0.0 0.0 0.0 0.06421377554469053 0.0 30-31 0.0 0.0 0.0 0.138566568280648 0.0 32-33 0.0 0.0 0.0 0.26068539756212966 0.0 34-35 0.0 0.0 0.0 0.44634206790888403 0.0 36-37 0.0 0.0 0.0 0.6923822184169615 0.0 38-39 0.0 0.0 0.0 0.9909199468264875 0.0 40-41 0.0 0.0 0.0 1.3475880404659442 0.0 42-43 0.0 0.0 0.0 1.6605457044363834 0.0 44-45 0.0 0.0 0.0 1.9462406777369714 0.0 46-47 0.0 0.0 0.0 2.3346776919090644 0.0 48-49 0.0 0.0 0.0 2.7368587071626527 0.0 50-51 0.0 0.0 0.0 3.0978077191717546 0.0 52-53 0.0 0.0 0.0 3.4121172521010297 0.0 54-55 0.0 0.0 0.0 3.715161210373341 0.0 56-57 0.0 0.0 0.0 4.0245138904535525 0.0 58-59 0.0 0.0 0.0 4.358425523285943 0.0 60-61 0.0 0.0 0.0 4.7410044386364145 0.0 62-63 0.0 0.0 0.0 5.082351350742401 0.0 64-65 0.0 0.0 0.0 5.436090394971048 0.0 66-67 0.0 0.0 0.0 5.831737376923597 0.0 68-69 0.0 0.0 0.0 6.255097672532276 0.0 70-71 0.0 0.0 0.0 6.731631480521822 0.0 72-73 0.0 0.0 0.0 7.205010927607417 0.0 74-75 0.0 0.0 0.0 7.647522700132933 0.0 76-77 0.0 0.0 0.0 8.139828312642228 0.0 78-79 0.0 0.0 0.0 8.700177996079582 0.0 80-81 0.0 0.0 0.0 9.299506567830026 0.0 82-83 0.0 0.0 0.0 9.910326025730573 0.0 84-85 0.0 0.0 0.0 10.567559651217808 0.0 86-87 0.0 0.0 0.0 11.258139377689655 0.0 88-89 0.0 0.0 0.0 11.898249329698308 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 20 1.7474156E-5 94.92651 2 TAACGCA 20 1.7513325E-5 94.883736 4 GTAGATA 60 1.4994002E-6 47.441868 6 TGTAGAT 65 2.6017224E-6 43.79249 5 TAGATAT 70 4.33075E-6 40.66445 7 GGACTGG 55 0.0025906723 34.503174 6 CATGGGG 235 0.0 34.31965 4 GAACTGC 70 2.0628018E-4 33.887043 7 AGATATA 85 1.6399861E-5 33.488377 8 ACTGGAA 85 1.6399861E-5 33.488377 3 GTACTGG 75 3.023763E-4 31.74953 1 ATGGGGC 75 3.09264E-4 31.627913 5 CTCCGAC 30 0.003972101 31.627913 94-95 CAGGGGA 60 0.003967859 31.627913 4 TCTATAG 75 3.09264E-4 31.627913 3 CATGTAG 90 2.4244702E-5 31.62791 3 GAGTAAT 65 0.005759974 29.307257 1 TGTATGG 65 0.005856146 29.208157 2 CCTGGGC 65 0.0058690645 29.194992 3 ACTGGTA 65 0.0058690645 29.194992 8 >>END_MODULE