##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936348_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 539302 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08466870139551 33.0 31.0 34.0 30.0 34.0 2 32.174568238204195 34.0 31.0 34.0 30.0 34.0 3 32.2244178586395 34.0 31.0 34.0 30.0 34.0 4 35.73294925663172 37.0 35.0 37.0 35.0 37.0 5 35.7702715732558 37.0 35.0 37.0 35.0 37.0 6 35.89892305238994 37.0 36.0 37.0 35.0 37.0 7 35.852450018727914 37.0 36.0 37.0 35.0 37.0 8 35.86580246318389 37.0 36.0 37.0 35.0 37.0 9 37.57374903115509 39.0 38.0 39.0 35.0 39.0 10-11 37.53751608560695 39.0 38.0 39.0 35.0 39.0 12-13 37.497557954541236 39.0 38.0 39.0 35.0 39.0 14-15 38.7438392588939 40.0 38.0 41.0 35.0 41.0 16-17 38.734700594472116 40.0 38.0 41.0 35.5 41.0 18-19 38.81270234488284 40.0 38.5 41.0 35.5 41.0 20-21 38.787623632028065 40.0 39.0 41.0 35.0 41.0 22-23 38.64302561459071 40.0 38.0 41.0 35.0 41.0 24-25 38.76721206299995 40.0 39.0 41.0 35.0 41.0 26-27 38.684459727573795 40.0 38.0 41.0 35.0 41.0 28-29 38.55943608590363 40.0 38.0 41.0 35.0 41.0 30-31 38.35898439093495 40.0 38.0 41.0 34.5 41.0 32-33 38.08766424007328 40.0 38.0 41.0 34.0 41.0 34-35 37.809987724874006 40.0 37.0 41.0 33.5 41.0 36-37 37.586148391810156 40.0 37.0 41.0 33.0 41.0 38-39 37.39938661454992 40.0 37.0 41.0 33.0 41.0 40-41 37.24616912230995 40.0 37.0 41.0 33.0 41.0 42-43 36.99350085851712 40.0 37.0 41.0 32.5 41.0 44-45 36.69355852564982 40.0 37.0 41.0 31.5 41.0 46-47 36.24664288283745 40.0 36.5 41.0 30.0 41.0 48-49 35.92988993180074 40.0 36.5 41.0 28.5 41.0 50-51 35.552949924161226 39.5 36.0 40.5 27.5 41.0 52-53 35.58547993517547 39.5 36.0 40.5 27.0 41.0 54-55 35.69370686554102 40.0 36.0 41.0 27.0 41.0 56-57 35.566244886909374 40.0 36.0 41.0 26.0 41.0 58-59 35.39670351676797 40.0 36.0 41.0 25.5 41.0 60-61 35.184283573952996 39.5 35.5 41.0 24.0 41.0 62-63 34.91609710329278 39.0 35.0 41.0 22.5 41.0 64-65 34.63825463284023 39.0 35.0 41.0 22.0 41.0 66-67 34.31556715903149 38.5 35.0 40.5 20.5 41.0 68-69 33.91240992987231 37.5 35.0 40.0 18.5 41.0 70-71 33.5008733511094 37.0 34.5 39.5 13.0 41.0 72-73 33.07701714438292 36.5 34.0 39.0 7.5 41.0 74-75 32.626166785956656 36.0 34.0 39.0 2.0 40.5 76-77 32.22540524604025 36.0 34.0 37.5 2.0 40.0 78-79 31.800766546387738 35.0 33.5 37.0 2.0 39.0 80-81 31.348733177329215 35.0 33.0 37.0 2.0 39.0 82-83 30.947685155997938 35.0 32.5 36.0 2.0 38.5 84-85 30.582306388628265 35.0 32.0 36.0 2.0 37.0 86-87 30.285463432362572 35.0 32.0 35.5 2.0 37.0 88-89 29.93246733741021 35.0 32.0 35.0 2.0 36.5 90-91 29.596618406755397 35.0 31.0 35.0 2.0 36.0 92-93 29.30991633630137 35.0 31.0 35.0 2.0 36.0 94-95 28.982049018917046 34.0 31.0 35.0 2.0 36.0 96-97 28.657805830499424 34.0 30.5 35.0 2.0 35.0 98-99 28.34660264564196 34.0 30.0 35.0 2.0 35.0 100-101 25.443783816859572 31.0 24.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4440395216805939 1101 2 0.3647923636704107 1101 3 0.3991341979962115 1101 4 0.2562110702529381 1101 5 0.487301470757906 1101 6 0.336007884218553 1101 7 0.47420617967296863 1101 8 0.12584545999466457 1101 9 0.3849665558135982 1101 10-11 0.6026627015463006 1101 12-13 0.3284892771483783 1101 14-15 0.6211834838229464 1101 16-17 0.6217494021165493 1101 18-19 0.5695575601359693 1101 20-21 0.6910783552292799 1101 22-23 0.5767764617379996 1101 24-25 0.5439306858050159 1101 26-27 0.6143743717263703 1101 28-29 0.649066746285051 1101 30-31 0.7350595130763864 1101 32-33 0.7903146230739111 1101 34-35 0.5960653459587277 1101 36-37 0.8531311809187514 1101 38-39 0.6887729688859139 1101 40-41 0.9727044314238213 1101 42-43 1.0182542883053571 1101 44-45 1.2262152920634861 1101 46-47 1.2536021740834755 1101 48-49 1.5208552446773993 1101 50-51 1.6118617680917495 1101 52-53 1.7446168485513383 1101 54-55 1.7010344031757612 1101 56-57 1.316928629430997 1101 58-59 1.1395032712800415 1101 60-61 1.0942483845390072 1101 62-63 1.199174574647607 1101 64-65 1.2935541578290852 1101 66-67 1.285789494856182 1101 68-69 1.2286780800470964 1101 70-71 1.3298880169767457 1101 72-73 1.1795308184498197 1101 74-75 1.3459415381749764 1101 76-77 1.4198413852656486 1101 78-79 1.2968086882834662 1101 80-81 1.3814416743504943 1101 82-83 1.486935004325769 1101 84-85 1.2770986164245741 1101 86-87 1.0152812205908681 1101 88-89 0.98439024732523 1101 90-91 1.1264327263294653 1101 92-93 1.3421414122085658 1101 94-95 1.5430612498742455 1101 96-97 1.1705131106238937 1101 98-99 1.3916988248977376 1101 100-101 1.3485454264012695 1102 1 0.2645558399290664 1102 2 0.21780308335575427 1102 3 0.3129390610729743 1102 4 0.21079734496334623 1102 5 0.22241838694428395 1102 6 0.1565185186465925 1102 7 0.2871402814588251 1102 8 0.14835907274461135 1102 9 0.36104903941577504 1102 10-11 0.3634051221717485 1102 12-13 0.3512217169851368 1102 14-15 0.6572530083145267 1102 16-17 0.38035186434755985 1102 18-19 0.51290407993271 1102 20-21 0.37780038230700086 1102 22-23 0.2603610199346349 1102 24-25 0.4783024531321374 1102 26-27 0.5329053314962877 1102 28-29 0.4720725778848234 1102 30-31 0.536816381973388 1102 32-33 0.327410077154326 1102 34-35 0.123282320120083 1102 36-37 0.11827856238267742 1102 38-39 0.22838635541980778 1102 40-41 0.14701015275746698 1102 42-43 0.015705094839475464 1102 44-45 -0.10395783710701778 1102 46-47 0.415926401936467 1102 48-49 0.5889218363176312 1102 50-51 0.3975305832425349 1102 52-53 0.5340227756392935 1102 54-55 0.8157992635242977 1102 56-57 0.47813314465795287 1102 58-59 0.46844869523621213 1102 60-61 0.5655593008845372 1102 62-63 0.7241777007488466 1102 64-65 0.3101992259780886 1102 66-67 0.2269420599643368 1102 68-69 0.1835826487019645 1102 70-71 0.3282141318581253 1102 72-73 0.43869597890407164 1102 74-75 0.6094264021611551 1102 76-77 0.47454815696932684 1102 78-79 0.36736479327499794 1102 80-81 0.06582196740827939 1102 82-83 0.06053763707139481 1102 84-85 0.054795873412828655 1102 86-87 0.1390918811644788 1102 88-89 0.02249457953316991 1102 90-91 -0.033592396156866045 1102 92-93 0.014653888194427367 1102 94-95 0.11635127249585153 1102 96-97 0.17012376892397896 1102 98-99 0.012661891432539818 1102 100-101 0.46219057412375975 1103 1 0.1288501508291091 1103 2 0.19454033519628666 1103 3 0.1672310950319229 1103 4 0.05867599484570718 1103 5 0.12261789035427739 1103 6 0.032712557397964304 1103 7 2.673889846604993E-4 1103 8 0.24679340467925215 1103 9 0.1972285491144632 1103 10-11 0.15756151549705777 1103 12-13 0.194749782994748 1103 14-15 0.18892417465049505 1103 16-17 0.39263108238093025 1103 18-19 0.2978063341043864 1103 20-21 0.330334292465416 1103 22-23 0.16145232527243536 1103 24-25 0.38428376053003177 1103 26-27 0.34592238686932575 1103 28-29 0.20165329544654753 1103 30-31 0.2870169696410372 1103 32-33 0.3832357935775761 1103 34-35 0.30309832159467476 1103 36-37 0.3749033767035499 1103 38-39 0.46066958520653145 1103 40-41 0.5309891615291136 1103 42-43 0.5363358075220361 1103 44-45 0.6004247323667897 1103 46-47 0.6836079337756971 1103 48-49 0.7811905370516783 1103 50-51 0.685569052704416 1103 52-53 0.7612696285814238 1103 54-55 0.8034143303687742 1103 56-57 1.1408693772962764 1103 58-59 1.0434142700554077 1103 60-61 0.7506827436194712 1103 62-63 0.750254097543781 1103 64-65 0.6211995620979636 1103 66-67 0.7229221746787999 1103 68-69 0.8950996335982566 1103 70-71 0.7426147000091348 1103 72-73 0.794842093466734 1103 74-75 0.7150818901313158 1103 76-77 0.7479816757107116 1103 78-79 0.9687998082919655 1103 80-81 0.8979490640915309 1103 82-83 0.8437647131537567 1103 84-85 1.094558312558867 1103 86-87 1.219467901722009 1103 88-89 1.0169269164337074 1103 90-91 1.1289351168813333 1103 92-93 1.1414496899487538 1103 94-95 1.1995599161979378 1103 96-97 1.112275911959248 1103 98-99 1.3342472929873637 1103 100-101 1.6704954456934402 1104 1 0.12074892686229788 1104 2 0.21060328501312853 1104 3 0.20494208593354557 1104 4 0.18259682191454374 1104 5 0.12702499752499818 1104 6 0.0271584657115298 1104 7 0.046317267311302146 1104 8 0.25320515145400435 1104 9 0.016971149256029605 1104 10-11 0.127631419553353 1104 12-13 0.13252587301752783 1104 14-15 0.31528621876289264 1104 16-17 0.3701744688785453 1104 18-19 0.11265358366652123 1104 20-21 0.028452617868026664 1104 22-23 0.17762635692300677 1104 24-25 0.2498230757492763 1104 26-27 0.2412032172447809 1104 28-29 0.18286754905412295 1104 30-31 0.023417194904659766 1104 32-33 -0.024899529928887887 1104 34-35 0.17491763536727234 1104 36-37 0.01616588632633409 1104 38-39 0.04211323571725245 1104 40-41 -0.06710901414550818 1104 42-43 -0.08317791237607963 1104 44-45 -0.1509534573609841 1104 46-47 0.25422239714710315 1104 48-49 0.21813222167227053 1104 50-51 0.08469383118657703 1104 52-53 -0.09194218639240859 1104 54-55 0.054975934287128325 1104 56-57 0.17812054107223219 1104 58-59 0.11247617105308194 1104 60-61 0.17288671053258753 1104 62-63 0.42411783371667866 1104 64-65 0.16923599693942748 1104 66-67 0.2063115655886918 1104 68-69 0.33069800302026664 1104 70-71 0.31659520127237073 1104 72-73 0.33384990019467153 1104 74-75 0.1435348560162737 1104 76-77 0.1259802393967746 1104 78-79 0.3724298592862745 1104 80-81 0.4918467964721458 1104 82-83 0.5715263883711366 1104 84-85 0.29786390126675144 1104 86-87 0.3972763346414965 1104 88-89 0.2367397822933519 1104 90-91 0.2530367748422826 1104 92-93 0.18961528971315644 1104 94-95 0.18366229748246 1104 96-97 0.3588388333598118 1104 98-99 0.4102892664207225 1104 100-101 0.4858774380365425 1105 1 0.07453746883292922 1105 2 0.09405333850987319 1105 3 0.20451897534119468 1105 4 0.18827233986026926 1105 5 -0.016793697154511733 1105 6 0.2435771020309545 1105 7 0.2047357090971218 1105 8 0.13132498415509986 1105 9 0.013916971646843024 1105 10-11 0.22600463227585266 1105 12-13 0.06563792437439986 1105 14-15 0.04972596197853818 1105 16-17 0.17438125810139127 1105 18-19 -0.05624778779539241 1105 20-21 0.1418389616089044 1105 22-23 0.19479151595422906 1105 24-25 0.22127040577553458 1105 26-27 0.1871715082670633 1105 28-29 0.4299568399791269 1105 30-31 0.5990688824836923 1105 32-33 0.6411883992608765 1105 34-35 0.2511553554334114 1105 36-37 0.3734122631379293 1105 38-39 0.4372912339664481 1105 40-41 0.5041340555947897 1105 42-43 0.6466757010856412 1105 44-45 0.840893152890942 1105 46-47 0.7708355670722042 1105 48-49 0.4147230202327208 1105 50-51 0.4369723060431099 1105 52-53 0.5192799709852736 1105 54-55 0.5175598269045807 1105 56-57 0.5356806033229802 1105 58-59 0.5570024817232522 1105 60-61 0.6690957368918831 1105 62-63 0.5713505931758789 1105 64-65 0.3620771602503936 1105 66-67 0.5268859260594638 1105 68-69 0.5230090622557455 1105 70-71 0.6838989654976402 1105 72-73 0.7597155747399498 1105 74-75 0.8063084188992633 1105 76-77 0.7208178038360344 1105 78-79 0.4193294799457483 1105 80-81 0.7746962274613516 1105 82-83 0.6275155917560227 1105 84-85 0.5109706029494703 1105 86-87 0.634835910558234 1105 88-89 0.7855482639585638 1105 90-91 0.6838703513426232 1105 92-93 0.5591221470227588 1105 94-95 0.8790834627387589 1105 96-97 0.9977212377951759 1105 98-99 1.1793948981686064 1105 100-101 0.9991981266855099 1106 1 0.1345819668397894 1106 2 0.0922687830264195 1106 3 0.13141709344631636 1106 4 0.08671954482920796 1106 5 0.023458458217696432 1106 6 0.09951480482135366 1106 7 -0.016632604613825208 1106 8 -0.07620723054778011 1106 9 0.07619564041480231 1106 10-11 0.15502799372945475 1106 12-13 0.10408466582244102 1106 14-15 0.021458139599744186 1106 16-17 0.011977438689129372 1106 18-19 0.08603690674432585 1106 20-21 0.023773234344602656 1106 22-23 -0.02096124199515259 1106 24-25 -0.10821415897833475 1106 26-27 -0.07703540746484094 1106 28-29 0.1839408021558242 1106 30-31 0.19368045196547712 1106 32-33 0.16655689838018617 1106 34-35 -0.07577520913505253 1106 36-37 -0.20167348822945286 1106 38-39 -0.24438810227161412 1106 40-41 -0.12459960529331937 1106 42-43 -0.049289349438133456 1106 44-45 -0.2620756985042547 1106 46-47 -0.17211427996588924 1106 48-49 0.062071680657304285 1106 50-51 0.1806521985062588 1106 52-53 0.06847576221546348 1106 54-55 0.019851937778774698 1106 56-57 -0.06847486158848426 1106 58-59 -0.07349951141664945 1106 60-61 -0.25828676055874666 1106 62-63 -0.26409021520458253 1106 64-65 -0.32534288239353515 1106 66-67 -0.37139672898828024 1106 68-69 -0.32213323866202614 1106 70-71 -0.2662957132823678 1106 72-73 -0.32643210302031633 1106 74-75 -0.5149416969809621 1106 76-77 -0.5412731389654937 1106 78-79 -0.6332082970079931 1106 80-81 -0.5421967919138204 1106 82-83 -0.6138745074374512 1106 84-85 -0.4269778535259192 1106 86-87 -0.4381176446471038 1106 88-89 -0.47115888353379276 1106 90-91 -0.6954566163036198 1106 92-93 -0.571757615654537 1106 94-95 -0.49885989500667804 1106 96-97 -0.3921879121074845 1106 98-99 -0.42440829085521514 1106 100-101 -0.3573560589082625 1107 1 -0.185484875455316 1107 2 -0.0562818258137483 1107 3 0.019652575564634844 1107 4 -0.1258836977526201 1107 5 -0.047926738770748045 1107 6 -0.09521816842803332 1107 7 -0.13496264301161887 1107 8 0.06010722044594985 1107 9 -0.0963707110642602 1107 10-11 -0.1587244432292252 1107 12-13 -0.1137052466525077 1107 14-15 -0.046705934492926815 1107 16-17 -0.4471204642708315 1107 18-19 -0.22895284319061204 1107 20-21 0.08637624863990823 1107 22-23 0.15331725843631006 1107 24-25 -0.009802120060044217 1107 26-27 0.006136222911884204 1107 28-29 -0.010721402270199576 1107 30-31 -0.2394726872025572 1107 32-33 -0.12117544023265481 1107 34-35 -0.25962576364302237 1107 36-37 -0.1590660908328303 1107 38-39 -0.3119243884150791 1107 40-41 -0.4342748448800293 1107 42-43 -0.9282358527733976 1107 44-45 -0.6561439013870611 1107 46-47 -0.802137154275897 1107 48-49 -0.7876929559333732 1107 50-51 -0.7622898669389215 1107 52-53 -0.667013204696687 1107 54-55 -0.7249990596050608 1107 56-57 -0.618362316330682 1107 58-59 -0.5653953397086511 1107 60-61 -0.6854359640670609 1107 62-63 -0.7833370522947476 1107 64-65 -0.9231476791500342 1107 66-67 -0.5991102567912989 1107 68-69 -0.500094000773764 1107 70-71 -0.3933052054361639 1107 72-73 -0.6300828611598703 1107 74-75 -0.5612062445398962 1107 76-77 -0.5132874936223075 1107 78-79 -0.7443587241320877 1107 80-81 -0.7855844726596821 1107 82-83 -0.5841150757795894 1107 84-85 -0.4911195190131039 1107 86-87 -0.6610257410570703 1107 88-89 -0.3916512519151958 1107 90-91 -0.3710500471305167 1107 92-93 -0.41391574516605445 1107 94-95 -0.3924578276438879 1107 96-97 -0.19465842890252105 1107 98-99 -0.3589790893558309 1107 100-101 -0.6165685617047707 1108 1 0.083915132942046 1108 2 0.190600653087877 1108 3 0.0637261364665136 1108 4 0.017811981804001675 1108 5 0.08798038639195482 1108 6 0.10887974482720608 1108 7 0.2800980279376972 1108 8 0.1394665016316594 1108 9 0.10965439705179136 1108 10-11 0.11622202358019962 1108 12-13 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2316 56-57 -1.981843535396088 2316 58-59 -2.0946015290177726 2316 60-61 -2.075780855692237 2316 62-63 -1.812989234282277 2316 64-65 -1.6709057569637977 2316 66-67 -1.6322853588631219 2316 68-69 -1.7751941029159397 2316 70-71 -1.5892113584468248 2316 72-73 -1.6049919039779823 2316 74-75 -1.7088846908533526 2316 76-77 -2.155093468517279 2316 78-79 -2.1398332350174023 2316 80-81 -1.8061142050359358 2316 82-83 -1.9680894473801267 2316 84-85 -2.1322248492587264 2316 86-87 -2.0217972380448614 2316 88-89 -2.131460248867814 2316 90-91 -2.0570712015340256 2316 92-93 -2.2195557939016552 2316 94-95 -2.1896690368327434 2316 96-97 -2.4203813731857977 2316 98-99 -2.3371465518685284 2316 100-101 -2.5970053812845535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2674.0 3 409.0 4 315.0 5 282.0 6 345.0 7 365.0 8 481.0 9 578.0 10 803.0 11 1265.0 12 2049.0 13 3557.0 14 3959.0 15 3980.0 16 5296.0 17 7735.0 18 6610.0 19 3071.0 20 2557.0 21 2368.0 22 2431.0 23 2472.0 24 2733.0 25 2946.0 26 3401.0 27 4049.0 28 4644.0 29 5486.0 30 6895.0 31 8549.0 32 10924.0 33 14538.0 34 20334.0 35 33558.0 36 50142.0 37 104160.0 38 171641.0 39 41689.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.40723411912903 23.586671589098174 14.430206909090234 20.575887382682563 2 13.909998924408146 24.096039225722222 40.92552824541297 21.06843360445666 3 16.410755679184053 31.010477515067226 32.2794622160408 20.29930458970793 4 9.957219707491836 19.891630676455264 43.49774786886644 26.653401747186457 5 8.949367276395627 42.69252451525806 37.13801979022951 11.220088418116813 6 24.35602401036963 43.99063914535842 18.939412202118802 12.713924642153145 7 23.82404972685094 37.49151624615681 24.4391450601373 14.245288966854947 8 24.153079844524225 36.12456606236834 22.245608699522297 17.47674539358514 9 26.294530341554605 17.206436812305387 24.026586994701518 32.47244585143849 10-11 21.347498924507867 29.72817863553426 30.351315067273887 18.573007372683982 12-13 22.94744669842724 29.170522380448148 30.139287853528003 17.74274306759661 14-15 21.135242743742833 30.193336380823805 28.87018154203636 19.801239333397003 16-17 17.28125449175372 33.39323883842669 30.23067328780144 19.094833382018148 18-19 17.0550103897284 31.823260566599505 34.192823344945474 16.92890569872662 20-21 18.0227081394012 31.077755814255358 33.90857776508591 16.990958281257534 22-23 18.409889373207566 30.519679811885307 33.19786627797543 17.872564536931694 24-25 18.13623435432283 31.365635661711178 33.43373521908353 17.06439476488246 26-27 17.120077438807858 31.047472993308478 34.38173660606252 17.450712961821147 28-29 17.345262072470057 30.530951294691043 34.969547590698845 17.154239042140052 30-31 17.871823522048153 30.5922007353248 34.33076634196776 17.20520940065929 32-33 16.832801393120818 31.904172871605518 34.36262682647745 16.90039890879621 34-35 17.418253014960452 30.997753887002666 34.17643143219453 17.407561665842348 36-37 18.025582738638693 30.50215208034433 32.97219996646108 18.50006521455589 38-39 16.668312514905796 31.957466312902454 33.78705953374672 17.58716163844503 40-41 16.48177983355992 31.6850217904003 34.58090402439854 17.252294351641247 42-43 17.07483646033167 31.16966850432363 33.32771197265969 18.427783062685005 44-45 17.522562609202097 30.771904484566104 33.309260337798484 18.396272568433314 46-47 18.03814454894155 30.933596502018496 33.04509652624791 17.98316242279205 48-49 17.554229050461558 31.657370258016115 32.519629634807245 18.26877105671508 50-51 17.421422220068113 31.084197202685093 32.34723912885432 19.147141448392464 52-53 17.32161795926584 32.0480966555639 32.263566093759025 18.366719291411233 54-55 18.529641985202304 31.15455578955216 31.487969882773914 18.827832342471627 56-57 18.60854089210671 31.675938091111544 30.918290330696337 18.79723068608541 58-59 18.0972108465113 30.62943943913164 32.13923311091295 19.134116603444117 60-61 18.317525896150773 32.272981814691924 31.054602512604486 18.354889776552813 62-63 17.75270597508742 32.08289037365144 31.85564157488356 18.308762076377587 64-65 17.914204533276425 31.21666742771089 32.115196624318436 18.753931414694243 66-67 18.25410430171439 31.811296215899965 30.385900319176883 19.548699163208763 68-69 18.043468334353737 30.871034746897546 31.537513174411032 19.54798374433768 70-71 18.56422105964399 30.6762882179477 32.432858001369624 18.32663272103868 72-73 18.70815109121641 30.07139356283882 31.98668289219404 19.233772453750724 74-75 18.002659715744045 31.53179683648526 31.146725166744794 19.3188182810259 76-77 17.70989023276617 30.85186082483833 32.314057287687056 19.124191654708437 78-79 17.491152578191826 30.877269161027737 32.07733177785191 19.554246482928527 80-81 18.53598491201783 31.130704118258766 30.823484026791604 19.509826942931795 82-83 18.88181741942097 30.71694139791459 31.081841646710213 19.31939953595423 84-85 17.628630718926843 30.20996692971936 31.629594220481582 20.53180813087221 86-87 18.33718184545941 30.70059693870895 31.31132692597997 19.65089428985167 88-89 18.742742324804613 30.101901021619714 31.53209406879018 19.62326258478549 90-91 18.214333987406377 29.529486886690005 32.295143857228744 19.961035268674873 92-93 19.34572548917263 30.385605886196355 31.411067189991826 18.857601434639193 94-95 18.6640513618532 29.124928948815192 32.17812648508624 20.032893204245365 96-97 19.266737899368128 29.050645705497185 31.604123795852168 20.07849259928252 98-99 18.607787833512795 29.57016390144303 31.535472236608257 20.286576028435913 100-101 18.442914632783097 29.288761784104032 31.614003055483103 20.654320527629764 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1758.0 1 1023.5 2 497.5 3 673.0 4 485.0 5 478.5 6 939.5 7 1450.5 8 1846.5 9 1830.0 10 1359.0 11 1100.0 12 1150.0 13 1207.5 14 1208.0 15 1210.5 16 1202.0 17 1190.0 18 1187.0 19 1214.5 20 1368.0 21 1641.0 22 1993.0 23 2273.0 24 2407.0 25 3062.5 26 5074.5 27 7447.5 28 8016.0 29 8540.0 30 11029.5 31 13609.0 32 18088.0 33 23588.5 34 28025.0 35 31582.5 36 35567.0 37 39759.0 38 36906.0 39 31158.0 40 27405.0 41 23032.0 42 17864.0 43 17563.0 44 18947.0 45 16880.5 46 13081.5 47 10932.0 48 12475.0 49 11681.0 50 9917.5 51 6713.0 52 3063.5 53 1880.5 54 1553.0 55 1470.5 56 1298.0 57 1137.0 58 998.0 59 1026.0 60 955.0 61 792.0 62 755.0 63 734.0 64 682.0 65 596.0 66 413.0 67 389.5 68 295.5 69 135.0 70 202.5 71 199.5 72 167.0 73 183.5 74 186.5 75 148.5 76 77.5 77 48.0 78 42.0 79 43.5 80 26.5 81 9.0 82 1.5 83 1.5 84 2.0 85 2.5 86 1.0 87 0.0 88 1.5 89 3.0 90 1.5 91 1.5 92 2.0 93 1.5 94 2.5 95 4.5 96 3.5 97 1.0 98 1.5 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0506209878695054 2 0.011867191295415185 3 0.009642092927524838 4 0.0064898702396801785 5 0.008529543743579664 6 0.0064898702396801785 7 0.006675295103671042 8 0.010012942655506563 9 0.016873662623168466 10-11 0.0025959480958720714 12-13 0.010940066975460874 14-15 0.011218204271447166 16-17 0.02215827124690804 18-19 0.012516178319383202 20-21 0.004450196735780694 22-23 0.010476504815483718 24-25 0.025125069070761837 26-27 0.006953432399657335 28-29 0.018542486399086226 30-31 0.01566840100722786 32-33 0.015019413983259844 34-35 0.2751704981624396 36-37 0.48432974474413226 38-39 0.4817337966482602 40-41 0.5029649435752139 42-43 0.49295200091970737 44-45 0.5079714149029672 46-47 0.5125143240707433 48-49 0.5200240310623733 50-51 0.5301296861498752 52-53 0.5190041943104234 54-55 0.5065807284230357 56-57 0.5019451068232641 58-59 0.5016669695272779 60-61 0.4983293219754424 62-63 0.49424997496764334 64-65 0.5114944873187935 66-67 0.48377347015215966 68-69 0.5100110884068667 70-71 0.4931374257836981 72-73 0.5004617079113373 74-75 0.5152029845986108 76-77 0.5028722311432186 78-79 0.5015742570952825 80-81 0.5036139305991819 82-83 0.5037993554631728 84-85 0.5038920678951682 86-87 0.5063953035590448 88-89 0.5193750440384052 90-91 0.5413478904213224 92-93 0.5304078234458615 94-95 0.5037993554631728 96-97 0.4753366388405754 98-99 0.46652895780100945 100-101 0.4750585015445891 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 539302.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.07058753722404 #Duplication Level Percentage of deduplicated Percentage of total 1 72.22997842365783 11.607781910691967 2 8.786301907256343 2.824020678580832 3 3.6679781698185048 1.7683969278808533 4 2.229170753095109 1.4329633489213836 5 1.510343952278208 1.2136057348201934 6 1.1330464179810544 1.0925232986341604 7 0.9034372151518998 1.016313679533916 8 0.6772894575915264 0.8707551613010891 9 0.6576746010684328 0.9512295522731233 >10 5.970993088647613 20.590874871593282 >50 1.1342002330706482 13.038520161245462 >100 1.0165110939320865 31.95519393586525 >500 0.07038272046521825 7.74797794185818 >1k 0.012691965985531158 3.8898427968003086 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4985 0.9243429469944484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3380 0.6267360402891144 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2483 0.460409937289311 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2036 0.3775250230853956 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1654 0.3066927250408862 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1244 0.23066853080463265 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1119 0.20749042280577487 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1033 0.1915438845025607 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1017 0.1885770866787069 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 1015 0.1882062369507252 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1012 0.18764996235875261 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 996 0.18468316453489883 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 977 0.18116009211907244 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 953 0.17670989538329174 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 950 0.17615362079131913 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 931 0.17263054837549277 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 928 0.17207427378352017 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 907 0.16818035163971207 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 878 0.16280303058397708 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 877 0.1626176057199862 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 830 0.15390263711241567 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 819 0.15186296360851617 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 781 0.14481681877686342 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 768 0.14240629554498221 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 762 0.14129374636103706 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 761 0.1411083214970462 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 757 0.14036662204108274 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 746 0.13832694853718325 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 741 0.13739982421722893 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 740 0.13721439935323806 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 728 0.13498930098534773 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 726 0.134618451257366 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 724 0.13424760152938428 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 719 0.13332047720942997 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 707 0.13109537884153963 No Hit ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT 704 0.13053910424956702 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 704 0.13053910424956702 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 694 0.12868485560965842 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 680 0.12608890751378635 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 676 0.12534720805782287 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 666 0.12349295941791426 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 663 0.1229366848259417 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 660 0.1223804102339691 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 650 0.12052616159406047 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 640 0.11867191295415185 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 630 0.11681766431424323 No Hit ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG 629 0.11663223945025236 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 617 0.11440714108236201 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 610 0.11310916703442597 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 607 0.11255289244245338 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 606 0.11236746757846254 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 605 0.11218204271447167 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 605 0.11218204271447167 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 604 0.11199661785048082 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 602 0.11162576812249908 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 594 0.11014236921057219 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 590 0.10940066975460873 No Hit CCTCTAGATGGATATAAAGTACCGCCAAGTCCTTTGAGTTTTAAGCTATG 579 0.10736099625070926 No Hit TATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGA 564 0.10457962329084633 No Hit CGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACAT 558 0.10346707410690115 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 555 0.10291079951492854 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 554 0.1027253746509377 No Hit AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG 546 0.1012419757390108 No Hit GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG 543 0.1006857011470382 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.271243199543113E-5 0.0 12-13 0.0 0.0 0.0 3.708497279817245E-4 0.0 14-15 0.0 0.0 0.0 0.0010198367519497426 0.0 16-17 0.0 0.0 0.0 0.0017615362079131916 0.0 18-19 0.0 0.0 0.0 0.002132385935894916 0.0 20-21 0.0 0.0 0.0 0.0030595102558492273 0.0 22-23 0.0 0.0 0.0 0.005933595647707592 0.0 24-25 0.0 0.0 0.0 0.010940066975460874 0.0 26-27 0.0 0.0 0.0 0.018357061535095365 0.0 28-29 0.0 0.0 0.0 0.02855542905459279 0.0 30-31 0.0 0.0 0.0 0.06063393052501196 0.0 32-33 0.0 0.0 0.0 0.11830106322617012 0.0 34-35 0.0 0.0 0.0 0.20294751363799873 0.0 36-37 0.0 0.0 0.0 0.3267186103518993 0.0 38-39 0.0 0.0 0.0 0.49258115119172563 0.0 40-41 0.0 0.0 0.0 0.6830124865103411 0.0 42-43 0.0 0.0 0.0 0.8500802889661081 0.0 44-45 0.0 0.0 0.0 1.024472373549514 0.0 46-47 0.0 0.0 0.0 1.251339694642334 0.0 48-49 0.0 0.0 0.0 1.491557605942496 0.0 50-51 0.0 0.0 0.0 1.7237095356590557 0.0 52-53 0.0 0.0 0.0 1.9275841736170087 0.0 54-55 0.0 0.0 0.0 2.142028028822441 0.0 56-57 0.0 0.0 0.0 2.3779811682508134 0.0 58-59 0.0 0.0 0.0 2.6181990795509753 0.0 60-61 0.0 0.0 0.0 2.8855817334257985 0.0 62-63 0.0 0.0 0.0 3.141375333301193 0.0 64-65 0.0 0.0 0.0 3.4087579871760165 0.0 66-67 0.0 0.0 0.0 3.7126693392570393 0.0 68-69 0.0 0.0 0.0 4.059413834919952 0.0 70-71 0.0 0.0 0.0 4.454832357380466 0.0 72-73 0.0 0.0 0.0 4.795828682259661 0.0 74-75 0.0 0.0 0.0 5.16093023945767 0.0 76-77 0.0 0.0 0.0 5.574983960749265 0.0 78-79 0.0 0.0 0.0 6.055697920645575 0.0 80-81 0.0 0.0 0.0 6.559033713948771 0.0 82-83 0.0 0.0 0.0 7.041045647893018 0.0 84-85 0.0 0.0 0.0 7.542063630396327 0.0 86-87 0.0 0.0 0.0 8.068299394402395 0.0 88-89 0.0 0.0 0.0 8.64895735598978 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCAAG 25 5.3570264E-5 75.75734 1 ATGTATT 20 0.0020999447 71.02251 1 GCAAGCC 25 0.005096861 56.781113 9 GTATGGT 45 1.5438321E-5 52.609264 1 CCGTCCA 40 5.4887455E-4 47.317596 9 GATGGCC 60 1.5308433E-6 47.317593 9 ACCGTCC 45 9.80441E-4 42.060085 8 GCATACC 45 9.80441E-4 42.060085 8 ACACCGT 45 9.808886E-4 42.056183 6 ACTCCCG 165 0.0 40.14454 6 TACTCCC 170 0.0 38.971046 5 CAGGTAT 25 0.0016035361 38.058826 76-77 TGGTACT 175 0.0 37.8611 2 CACACCG 50 0.0016451204 37.85759 5 GGTAATC 50 0.0016458703 37.854076 8 TATACTC 130 2.1282176E-10 36.401527 5 CATTCCG 195 0.0 36.398148 9 GGGATAT 65 1.358374E-4 36.394775 7 CCTTTAG 185 0.0 35.831177 1 GTGGTAC 185 0.0 35.831177 1 >>END_MODULE