##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936348_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 539302 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46122395244223 34.0 31.0 34.0 31.0 34.0 2 32.58742411487441 34.0 31.0 34.0 31.0 34.0 3 32.666646888014505 34.0 31.0 34.0 31.0 34.0 4 36.16818591438563 37.0 35.0 37.0 35.0 37.0 5 36.15096179876952 37.0 35.0 37.0 35.0 37.0 6 36.211606854786375 37.0 37.0 37.0 35.0 37.0 7 36.15936710785422 37.0 36.0 37.0 35.0 37.0 8 36.14997719274173 37.0 36.0 37.0 35.0 37.0 9 37.898081223507425 39.0 38.0 39.0 35.0 39.0 10-11 37.857660457406055 39.0 38.0 39.0 35.0 39.0 12-13 37.83400673463106 39.0 38.0 39.0 35.0 39.0 14-15 39.10535655347097 40.5 38.5 41.0 36.0 41.0 16-17 39.05775706376019 40.0 38.0 41.0 36.0 41.0 18-19 39.189529243355295 40.5 39.0 41.0 36.0 41.0 20-21 39.178721755157596 40.0 39.0 41.0 36.0 41.0 22-23 39.0469087820924 40.0 38.5 41.0 35.0 41.0 24-25 39.15513293108499 41.0 39.0 41.0 35.0 41.0 26-27 39.04055798050072 41.0 39.0 41.0 35.0 41.0 28-29 38.9383119291232 40.5 38.0 41.0 35.0 41.0 30-31 38.759166478151386 40.0 38.0 41.0 35.0 41.0 32-33 38.5020164953959 40.0 38.0 41.0 35.0 41.0 34-35 38.32020648912854 40.0 38.0 41.0 34.0 41.0 36-37 38.14191770102836 40.0 38.0 41.0 34.0 41.0 38-39 37.96055549580754 40.0 37.0 41.0 34.0 41.0 40-41 37.832664258615765 40.0 37.0 41.0 33.5 41.0 42-43 37.70534783850236 40.0 38.0 41.0 33.0 41.0 44-45 37.42789197889124 40.0 38.0 41.0 33.0 41.0 46-47 37.04160006823635 40.0 37.0 41.0 32.5 41.0 48-49 36.68880237788845 40.0 37.0 41.0 31.0 41.0 50-51 36.463120848800855 40.0 37.0 41.0 30.5 41.0 52-53 36.316268065017375 40.0 37.0 41.0 30.0 41.0 54-55 36.161791537950904 40.0 36.5 41.0 29.5 41.0 56-57 35.94167646328032 40.0 36.0 41.0 28.0 41.0 58-59 35.74024943352704 40.0 36.0 41.0 28.0 41.0 60-61 35.525837100548486 39.5 35.5 41.0 27.0 41.0 62-63 35.261497268691755 39.0 35.0 41.0 26.5 41.0 64-65 34.929324385965565 39.0 35.0 40.5 26.0 41.0 66-67 34.59928110780231 38.5 35.0 40.0 26.0 41.0 68-69 34.2000437602679 37.5 35.0 40.0 24.0 41.0 70-71 33.82383525371684 37.0 34.5 39.5 23.0 41.0 72-73 33.4010767621852 36.5 34.0 39.0 22.0 41.0 74-75 32.939180644611 36.0 34.0 39.0 21.5 40.0 76-77 32.15725511865337 35.0 33.0 37.0 19.0 39.0 78-79 32.1384669443095 35.0 33.0 37.0 18.0 39.0 80-81 31.893523480350527 35.0 33.0 37.0 18.0 39.0 82-83 31.556178171043314 35.0 33.0 36.0 13.0 38.5 84-85 31.20452547923056 35.0 33.0 36.0 7.5 37.0 86-87 30.83965570311254 35.0 33.0 35.5 2.0 37.0 88-89 30.33222294743947 35.0 32.0 35.0 2.0 36.0 90-91 30.10685942199361 35.0 32.0 35.0 2.0 36.0 92-93 29.844380328647027 35.0 31.5 35.0 2.0 36.0 94-95 29.70633615302743 35.0 31.0 35.0 2.0 36.0 96-97 29.51824951511398 34.0 31.0 35.0 2.0 35.0 98-99 29.229963916321466 34.0 31.0 35.0 2.0 35.0 100-101 26.34544374024202 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.262120898604671 1101 2 0.27681918538625894 1101 3 0.2677644066509046 1101 4 0.2749500808947758 1101 5 0.23902400502040422 1101 6 0.2270271509645312 1101 7 0.22369563358137157 1101 8 0.18801590298782855 1101 9 0.2789732142345329 1101 10-11 0.2002097132641083 1101 12-13 0.2495764979487305 1101 14-15 0.3276085182737063 1101 16-17 0.48607876371378467 1101 18-19 0.49040839262232083 1101 20-21 0.3591830698768277 1101 22-23 0.2680293897794854 1101 24-25 0.2894911350471645 1101 26-27 0.5227769261155473 1101 28-29 0.243743188342755 1101 30-31 0.3715970903433643 1101 32-33 0.3473278390431602 1101 34-35 0.29296118062675447 1101 36-37 0.37816269777212597 1101 38-39 0.32799063266376294 1101 40-41 0.3934796707413071 1101 42-43 0.43032967728083094 1101 44-45 0.5888518370154046 1101 46-47 0.6382700826599432 1101 48-49 0.40315266950455 1101 50-51 0.28375346513510635 1101 52-53 0.3347153367730442 1101 54-55 0.003047570923655485 1101 56-57 0.13211978334725671 1101 58-59 0.03361978444897318 1101 60-61 0.14952279194986318 1101 62-63 0.20686671342498641 1101 64-65 0.1892867162747578 1101 66-67 0.253122817294134 1101 68-69 0.2521950297379547 1101 70-71 0.4149534441146443 1101 72-73 0.3753212947401252 1101 74-75 0.21985025895588706 1101 76-77 0.31376593063173885 1101 78-79 0.15973300369888932 1101 80-81 0.14145270091520246 1101 82-83 0.27604051502165916 1101 84-85 0.2685596774337178 1101 86-87 0.292040915154697 1101 88-89 0.4423004414545133 1101 90-91 0.3455187747901043 1101 92-93 0.41211311504841674 1101 94-95 0.396470859838562 1101 96-97 0.21590952155911225 1101 98-99 0.5434536334447984 1101 100-101 1.1702781532383497 1102 1 0.04914814683984048 1102 2 0.03843407244875152 1102 3 0.08592193941812809 1102 4 0.14097106555983174 1102 5 0.21443011399060907 1102 6 0.13507998775931185 1102 7 0.11708989746978204 1102 8 0.11355217144603813 1102 9 0.0917197499459732 1102 10-11 0.15378995175721855 1102 12-13 0.27295120949484186 1102 14-15 0.30886612038354144 1102 16-17 0.3209666219365701 1102 18-19 0.48452563847028784 1102 20-21 0.33107089399668865 1102 22-23 0.4353866406292184 1102 24-25 0.39629864678425264 1102 26-27 0.3912220601968741 1102 28-29 0.35677448345808216 1102 30-31 0.4154619745639394 1102 32-33 0.19080679185608318 1102 34-35 0.39925430661431704 1102 36-37 0.24426893974373343 1102 38-39 0.2139163564982951 1102 40-41 0.03917303999639188 1102 42-43 0.09140914845817605 1102 44-45 -0.0023535876228280017 1102 46-47 0.08630859622722653 1102 48-49 0.16015229437239498 1102 50-51 -0.07291763491149794 1102 52-53 0.14165016608792058 1102 54-55 0.20269460567453024 1102 56-57 0.3712921054152645 1102 58-59 0.34119404356297167 1102 60-61 0.24768521280266498 1102 62-63 0.012781296971461131 1102 64-65 0.23966810062605504 1102 66-67 0.23236550505914266 1102 68-69 0.1416276707819648 1102 70-71 -0.006558679474345297 1102 72-73 -0.13038241906814818 1102 74-75 -0.023979953960022726 1102 76-77 -0.21493736257382068 1102 78-79 -0.03666572767238563 1102 80-81 0.045867892630461427 1102 82-83 0.23126337762098714 1102 84-85 0.3043734300056471 1102 86-87 0.1891751328904938 1102 88-89 0.32755263256728995 1102 90-91 0.27019962827258226 1102 92-93 0.46491864881599554 1102 94-95 0.29889103058053124 1102 96-97 0.16037291207167925 1102 98-99 0.30590972593192234 1102 100-101 0.43726709252377916 1103 1 0.06359045891088044 1103 2 0.1018251967155166 1103 3 0.1103123155471124 1103 4 -6.763555333222371E-4 1103 5 -0.11068996754556792 1103 6 -0.025840113293504885 1103 7 0.13979425293281622 1103 8 0.08117081634080847 1103 9 0.17675265716604827 1103 10-11 0.08944468074701462 1103 12-13 0.09513566340847746 1103 14-15 0.458149426068303 1103 16-17 0.2757828944755758 1103 18-19 0.05824973826536706 1103 20-21 0.26911098984506054 1103 22-23 0.24322636566147082 1103 24-25 0.2386828209594043 1103 26-27 0.34870560658684013 1103 28-29 0.25094280361768284 1103 30-31 0.37030535354451644 1103 32-33 0.4813553178077896 1103 34-35 0.5587486088402969 1103 36-37 0.4483750235134849 1103 38-39 0.2816390901287136 1103 40-41 0.3055968329465415 1103 42-43 0.47898789267095054 1103 44-45 0.6856594017839726 1103 46-47 0.5987938232165675 1103 48-49 0.3330046510064264 1103 50-51 0.4555712351835908 1103 52-53 0.4287968336219876 1103 54-55 0.3770025782211306 1103 56-57 0.502744793832143 1103 58-59 0.6452795264371289 1103 60-61 0.3950981740187629 1103 62-63 0.7407789983452133 1103 64-65 0.6403880477142891 1103 66-67 0.7132663063255578 1103 68-69 0.6940081584810613 1103 70-71 0.6100532321803414 1103 72-73 0.6551053342415472 1103 74-75 0.80424652578062 1103 76-77 0.6662171549857305 1103 78-79 0.5980316018009901 1103 80-81 0.6093186510686976 1103 82-83 0.5477581894480643 1103 84-85 0.6983221879884951 1103 86-87 0.5931749594092643 1103 88-89 0.6298734860949224 1103 90-91 0.5252631115587505 1103 92-93 0.867771872856153 1103 94-95 0.7077374888528745 1103 96-97 0.7836530083537632 1103 98-99 0.558487548525683 1103 100-101 1.258703408649943 1104 1 -6.045077903991114E-4 1104 2 -0.0055366872323787675 1104 3 0.0606044866027986 1104 4 0.02911424229360904 1104 5 0.11078137592463122 1104 6 -0.0014641090362275122 1104 7 0.01752157028069945 1104 8 0.010502103701760745 1104 9 -0.0526700789603467 1104 10-11 -0.051097088605764895 1104 12-13 0.06381833619239075 1104 14-15 0.1431368454048041 1104 16-17 0.2120322551092002 1104 18-19 -0.022705668836643156 1104 20-21 -0.020128022094937137 1104 22-23 0.08725197131580131 1104 24-25 0.2111913587608072 1104 26-27 0.13679505071267073 1104 28-29 -0.02460214319220455 1104 30-31 -0.3220289666088263 1104 32-33 -0.2545566640471222 1104 34-35 -0.23120856007125923 1104 36-37 -0.1670648347150987 1104 38-39 0.15121351754061862 1104 40-41 0.1803016910976254 1104 42-43 0.20878004047695953 1104 44-45 0.06974679993744104 1104 46-47 0.11587975922309823 1104 48-49 0.35598050749902654 1104 50-51 0.3309012515617056 1104 52-53 0.1897564773334679 1104 54-55 0.0045426064307534375 1104 56-57 -0.06437446672450875 1104 58-59 -0.0883204032971534 1104 60-61 -0.12594020862973565 1104 62-63 -0.1982499914300888 1104 64-65 -0.2904544692450699 1104 66-67 -0.17436796061183912 1104 68-69 -0.23076102380164798 1104 70-71 -0.27506231875972986 1104 72-73 -0.23027371314131528 1104 74-75 -0.127187566525798 1104 76-77 -0.0491214379432634 1104 78-79 -0.03337304090300108 1104 80-81 0.019967523975580548 1104 82-83 0.2818825287694864 1104 84-85 0.10692250521754687 1104 86-87 0.06903145201328087 1104 88-89 0.032731130850049084 1104 90-91 0.2631022340639291 1104 92-93 0.23182320816312796 1104 94-95 0.27225020126459043 1104 96-97 0.392231625875759 1104 98-99 0.24125333118761816 1104 100-101 0.3422911968814688 1105 1 0.01176296619073014 1105 2 0.17080802631688385 1105 3 0.08283967773206768 1105 4 0.14676816700910678 1105 5 0.14597736853235688 1105 6 0.14375522760417425 1105 7 0.01810030775759941 1105 8 0.09655890751461982 1105 9 0.17241989283228065 1105 10-11 0.13366849839938766 1105 12-13 0.12332373449994805 1105 14-15 0.07338438941803105 1105 16-17 0.161434064271738 1105 18-19 0.2069996127507565 1105 20-21 0.22977471110222325 1105 22-23 0.1810439176245211 1105 24-25 0.2275346133080305 1105 26-27 0.12043720614502718 1105 28-29 0.16700616339276309 1105 30-31 0.1436309886484466 1105 32-33 0.25880536299305135 1105 34-35 0.1505198710152129 1105 36-37 0.1645638978984607 1105 38-39 0.17991451938868863 1105 40-41 0.5669031890063891 1105 42-43 0.44030177960738826 1105 44-45 0.20647474468989202 1105 46-47 0.3673047178847071 1105 48-49 0.32901328641158756 1105 50-51 0.2525212301630049 1105 52-53 0.3215797435527108 1105 54-55 0.388303913696582 1105 56-57 0.3072382168665797 1105 58-59 0.35540102457951406 1105 60-61 0.6510124224372831 1105 62-63 0.4073720297740735 1105 64-65 0.3545435854188739 1105 66-67 0.2619684852591888 1105 68-69 0.2087536148521778 1105 70-71 -0.1307013616543813 1105 72-73 -0.044528163097545814 1105 74-75 0.10898595745047146 1105 76-77 0.1573784161565257 1105 78-79 0.09781069608756354 1105 80-81 -0.05751034782677067 1105 82-83 0.12720642722294428 1105 84-85 0.08161368311852613 1105 86-87 0.39311471239651397 1105 88-89 0.6380394666150551 1105 90-91 0.3608967093095288 1105 92-93 0.40647304767290393 1105 94-95 0.36908903017228667 1105 96-97 0.2814690541414926 1105 98-99 0.18089441668513473 1105 100-101 0.5912066720297986 1106 1 -0.12358977321781595 1106 2 0.055576729385393264 1106 3 0.09357482188706712 1106 4 0.084823233292596 1106 5 0.06505125376457954 1106 6 0.05948712711284543 1106 7 0.10360600906472683 1106 8 0.07428672803932557 1106 9 -0.015125300455935076 1106 10-11 0.11067554391714651 1106 12-13 0.048842877913315874 1106 14-15 0.1976891080608496 1106 16-17 0.16355699001568524 1106 18-19 0.21546350447227525 1106 20-21 0.11674513846849521 1106 22-23 0.05956204129190468 1106 24-25 0.2042032885652887 1106 26-27 0.08110513976883027 1106 28-29 0.24849545605986378 1106 30-31 0.2138868521878905 1106 32-33 0.15904407625901484 1106 34-35 0.27364492895532777 1106 36-37 0.2897307654241885 1106 38-39 0.32415990735568556 1106 40-41 0.2901765736360389 1106 42-43 0.40387598930424673 1106 44-45 0.4847356142551078 1106 46-47 0.7221485113397961 1106 48-49 0.5367401798944798 1106 50-51 0.5720516834864426 1106 52-53 0.6361319823586769 1106 54-55 0.7471478925600294 1106 56-57 0.841599021538876 1106 58-59 0.8056374202409557 1106 60-61 0.8907500696051684 1106 62-63 0.6715047821612146 1106 64-65 0.7200228660344337 1106 66-67 0.5530758367007351 1106 68-69 0.6770441497553037 1106 70-71 0.5446740902784981 1106 72-73 0.37555619770712667 1106 74-75 0.5147799687603793 1106 76-77 0.5818082854436355 1106 78-79 0.37101918779053733 1106 80-81 0.4430319716318323 1106 82-83 0.48889874204634154 1106 84-85 0.6121504403262747 1106 86-87 0.5301963847799378 1106 88-89 0.5879583504567165 1106 90-91 0.8078258833152745 1106 92-93 0.9077222365113187 1106 94-95 0.8501588364205439 1106 96-97 1.041990978680289 1106 98-99 0.5543856561400347 1106 100-101 0.7095250181872146 1107 1 -0.14987468393495362 1107 2 -0.08289274642308442 1107 3 0.017706077508627516 1107 4 -0.01463207505401698 1107 5 -0.04309720552964791 1107 6 0.1143352480864408 1107 7 0.06419005731536487 1107 8 0.08392041947812601 1107 9 0.07660479181747348 1107 10-11 0.07859215541456166 1107 12-13 0.03564335092300297 1107 14-15 0.17892623840788957 1107 16-17 0.05019674931038054 1107 18-19 0.09322063500194133 1107 20-21 0.14386557787440069 1107 22-23 0.1362366371106063 1107 24-25 0.023966509779498324 1107 26-27 0.21248403604912625 1107 28-29 0.23125297467662875 1107 30-31 0.20219629914095094 1107 32-33 0.18028613759503997 1107 34-35 0.14688426911595087 1107 36-37 0.08412678775974314 1107 38-39 0.2924668888142534 1107 40-41 -0.011967493425416365 1107 42-43 -0.09100582676072833 1107 44-45 0.004007562863243663 1107 46-47 -0.12854296855044822 1107 48-49 -0.33337848344224597 1107 50-51 -0.2023875306600118 1107 52-53 -0.08082925002330654 1107 54-55 -0.20125478661065443 1107 56-57 -0.18448043130398162 1107 58-59 -0.17014594497639024 1107 60-61 -0.2965110856159683 1107 62-63 -0.17943553004344182 1107 64-65 -0.2071103574169868 1107 66-67 -0.26367856808779777 1107 68-69 -0.39786122547926084 1107 70-71 -0.2808898915864191 1107 72-73 -0.25220435712045486 1107 74-75 -0.18075429019872757 1107 76-77 -0.380236796579716 1107 78-79 -0.3756740818660731 1107 80-81 -0.16201179089538797 1107 82-83 0.09673673166387076 1107 84-85 0.001611821094506638 1107 86-87 0.0590769133089033 1107 88-89 -0.04454828126689492 1107 90-91 -0.15236322924368295 1107 92-93 0.0517504892519014 1107 94-95 -0.018407478532676436 1107 96-97 0.08881939396087546 1107 98-99 -0.19332120569639244 1107 100-101 0.25417101426981503 1108 1 -0.06449449331481105 1108 2 0.013689526743142721 1108 3 0.07167173229302648 1108 4 0.1076377322264932 1108 5 0.07654002837890062 1108 6 0.04460774252318345 1108 7 0.06605427195196967 1108 8 0.06011389472689643 1108 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>>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 13.0 6 32.0 7 69.0 8 133.0 9 305.0 10 500.0 11 898.0 12 1550.0 13 2374.0 14 2848.0 15 3946.0 16 5314.0 17 7335.0 18 6494.0 19 3151.0 20 2382.0 21 2316.0 22 2323.0 23 2395.0 24 2623.0 25 3048.0 26 3374.0 27 4031.0 28 4976.0 29 6095.0 30 7745.0 31 9441.0 32 11726.0 33 15106.0 34 20588.0 35 32278.0 36 47492.0 37 101397.0 38 182179.0 39 42817.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.44730299869283 23.89973242890958 14.702092409645948 20.95087216275165 2 13.858828198751084 24.17361694551241 40.6246812330416 21.342873622694906 3 16.300699793436703 30.849690896751724 32.53724258393256 20.312366725879006 4 9.890933095000575 20.282142473048495 43.09718858821217 26.729735843738762 5 8.96492132423021 42.68517454042448 37.14653385301742 11.2033702823279 6 24.436586550763764 43.824795754512316 18.838609906879633 12.900007787844286 7 23.581221653173916 37.5242443009668 24.332934051792872 14.561599994066404 8 23.967832494594866 36.543346770455145 22.10412718662271 17.38469354832728 9 25.94501781932943 17.241360128462347 23.980255960482253 32.83336609172597 10-11 21.305488596328573 29.6867235305025 30.24522529204524 18.762562581123678 12-13 22.639562471085284 29.24913058836291 30.211486524699076 17.899820415852727 14-15 20.837768077997115 30.319839347897837 28.830506840323235 20.011885733781813 16-17 17.19346261250737 33.54592451668651 30.116744360260935 19.14386851054519 18-19 16.93411112882949 31.957511746665134 34.00868159213205 17.099695532373328 20-21 17.933660484905012 31.028747370441213 33.836824114155064 17.200768030498708 22-23 18.20594027094281 30.663987895464878 33.09305361374518 18.037018219847138 24-25 17.982280471432915 31.59957870689719 33.26852614168206 17.149614679987835 26-27 17.002151889462787 31.37252720457413 34.12473912628281 17.500581779680267 28-29 17.1321685356277 30.66035592929625 34.96646068691851 17.24101484815754 30-31 17.742585970478316 30.74234398946038 34.21370539691841 17.301364643142897 32-33 16.681732590513754 31.907216160312558 34.37533898328656 17.03571226588713 34-35 17.331516146079604 31.173661910457174 34.035546407810195 17.459275535653028 36-37 17.874678751423136 30.443517732179743 32.95435581548001 18.72744770091711 38-39 16.537317252038054 31.954111012114744 33.72835046815186 17.780221267695342 40-41 16.310602921011423 31.650316753492262 34.59033431330828 17.448746012188032 42-43 16.989394261089018 31.229956043528123 33.19932836810323 18.58132132727962 44-45 17.369629271132915 30.88371911906232 33.274552234516186 18.472099375288575 46-47 17.90211372343937 30.993550157815285 32.90022216535185 18.20411395339349 48-49 17.372336006290475 31.673058520699964 32.479952821441024 18.474652651568537 50-51 17.348940707431275 31.055862036994114 32.27036298734412 19.324834268230497 52-53 17.194259679234822 32.06668978420949 32.23654809345174 18.502502443103957 54-55 18.45171375089352 31.14113771661201 31.38515796880954 19.02199056368493 56-57 18.498457681506363 31.635376808759 30.794820183372256 19.071345326362383 58-59 17.81297167799621 30.69434709566881 32.11026185893513 19.38241936739984 60-61 18.14120976011363 32.40700393289783 30.85118218718072 18.600604119807823 62-63 17.51940471201664 32.29350160021658 31.669083370727346 18.518010317039433 64-65 17.66585233567743 31.446650503664493 31.83048317487101 19.057013985787066 66-67 18.070301982933497 31.922373734938862 30.14229504062659 19.865029241501052 68-69 17.758973636292836 31.027420628886972 31.359701985158594 19.8539037496616 70-71 18.230618679903543 30.74300739011481 32.169667616351894 18.856706313629758 72-73 18.435684266447122 30.10802035614917 31.70352486989995 19.752770507503758 74-75 17.63214302932309 31.564411035004508 30.772368728467537 20.03107720720487 76-77 17.28389556531627 31.018632063201864 31.92896107853411 19.76851129294776 78-79 16.949534791990533 30.898814357641708 31.73326527297617 20.418385577391586 80-81 17.985594168688596 31.154534017064854 30.46344721956701 20.39642459467954 82-83 18.236257236205315 30.82836703739278 30.672239301912473 20.263136424489435 84-85 16.93267630574961 30.19780362415921 31.26734316402357 21.602176906067616 86-87 17.57150389312382 30.76997337749377 30.902900256571154 20.755622472811254 88-89 17.81554905484254 30.24876883810582 31.024067454896255 20.911614652155382 90-91 17.336352561539247 29.484437335515608 31.972231780011718 21.206978322933427 92-93 18.284449775223962 30.27191624477576 31.079994235319823 20.363639744680455 94-95 17.48761804807405 29.000283704776436 31.988918825202628 21.523179421946885 96-97 17.92976844447535 29.073082539461794 31.331525392300026 21.665623623762837 98-99 17.302052785923756 29.631748321640522 31.18378465078719 21.882414241648533 100-101 16.790670809100376 29.262196213029384 31.416762393228957 22.530370584641286 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1708.0 1 989.0 2 468.5 3 659.0 4 462.0 5 426.0 6 867.5 7 1355.5 8 1717.5 9 1737.0 10 1209.5 11 818.0 12 835.5 13 892.0 14 926.5 15 918.5 16 965.5 17 1005.0 18 1036.0 19 1139.5 20 1321.0 21 1554.0 22 1882.0 23 2254.5 24 2428.5 25 3005.5 26 4988.0 27 7410.5 28 7912.0 29 8389.5 30 11022.0 31 13662.5 32 18225.0 33 23638.0 34 27864.0 35 31495.5 36 35569.0 37 39809.5 38 36918.5 39 31025.0 40 27523.0 41 23399.5 42 17973.0 43 17457.5 44 18995.0 45 17271.5 46 13639.5 47 11338.0 48 12473.0 49 11261.5 50 9842.0 51 7390.0 52 3922.5 53 2439.5 54 1750.0 55 1548.5 56 1293.0 57 1040.0 58 945.5 59 1065.5 60 1014.5 61 795.0 62 749.5 63 757.5 64 721.0 65 574.5 66 373.0 67 384.5 68 290.0 69 140.0 70 200.5 71 194.0 72 195.0 73 210.0 74 167.0 75 129.0 76 63.5 77 32.5 78 23.5 79 32.5 80 23.5 81 5.0 82 0.0 83 0.0 84 0.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27832272085028426 2 0.021323859358949163 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.708497279817245E-4 12-13 9.271243199543113E-5 14-15 0.0 16-17 7.41699455963449E-4 18-19 0.0 20-21 9.271243199543113E-5 22-23 0.0 24-25 0.0018542486399086226 26-27 0.0015761113439223292 28-29 0.0017615362079131916 30-31 6.489870239680178E-4 32-33 0.0010198367519497424 34-35 0.0012979740479360357 36-37 0.0 38-39 9.271243199543113E-5 40-41 4.6356215997715565E-4 42-43 0.0034303599838309516 44-45 0.0024105232318812095 46-47 0.011867191295415185 48-49 0.013721439935323806 50-51 0.005006471327753281 52-53 0.005377321055735005 54-55 4.6356215997715565E-4 56-57 0.0030595102558492273 58-59 7.41699455963449E-4 60-61 9.271243199543113E-4 62-63 0.0 64-65 8.344118879588802E-4 66-67 0.0 68-69 0.0 70-71 9.271243199543113E-5 72-73 0.0010198367519497424 74-75 0.0 76-77 0.20211310175003985 78-79 0.10467233572284175 80-81 0.0012052616159406047 82-83 0.0 84-85 8.344118879588802E-4 86-87 0.1223804102339691 88-89 0.5788037129474766 90-91 0.30354050235304153 92-93 0.28638870243388675 94-95 0.0016688237759177604 96-97 0.0026886605278675026 98-99 0.0019469610719040538 100-101 0.0010198367519497424 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 539302.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.91168213728115 #Duplication Level Percentage of deduplicated Percentage of total 1 69.79818202166155 10.408083040671091 2 9.459207401236027 2.8210538807569785 3 3.9953244879941305 1.7873102640079215 4 2.366356209353511 1.4114540646984435 5 1.6563249978238974 1.2349295941791427 6 1.2509481590171476 1.1192244790488446 7 0.9736505054775613 1.016313679533916 8 0.795831830786257 0.9493753036332148 9 0.7038137753515961 0.9445542571694523 >10 6.5419863465101535 20.94689061045574 >50 1.2459742100747335 13.136053639704656 >100 1.1178950248075703 32.2483506458348 >500 0.08082667031422922 8.1351450578711 >1k 0.013678359591638793 3.8412614824347027 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4611 0.854994047861866 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3517 0.6521392466558626 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2331 0.4322253579626999 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2143 0.3973654835324178 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1699 0.315036843920475 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1244 0.23066853080463265 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1105 0.2048944747099028 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 1028 0.1906167601826064 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1022 0.18950421099866122 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1011 0.18746453749476175 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1005 0.18635198831081656 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 995 0.18449773967090793 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 975 0.1807892423910907 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 944 0.17504107160737398 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 929 0.17225969864751103 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 928 0.17207427378352017 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 926 0.17170342405553846 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 920 0.1705908748715933 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 890 0.16502812895186741 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 883 0.1637301549039314 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 831 0.15408806197640654 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 817 0.15149211388053446 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 788 0.14611479282479947 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 779 0.1444459690488817 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 748 0.13869779826516496 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 742 0.1375852490812198 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 733 0.13591642530530204 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 733 0.13591642530530204 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 732 0.13573100044131117 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 729 0.1351747258493386 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 726 0.134618451257366 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 715 0.13257877775346652 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 713 0.13220792802548478 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 709 0.13146622856952134 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 700 0.1297974047936036 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 689 0.1277577312897041 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 686 0.1272014566977315 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 685 0.12701603183374066 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 683 0.12664518210575892 No Hit ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT 676 0.12534720805782287 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 673 0.12479093346585031 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 672 0.12460550860185944 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 668 0.123863809145896 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 659 0.12219498536997822 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 658 0.12200956050598738 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 646 0.11978446213809703 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 644 0.11941361241011529 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 631 0.1170030891782341 No Hit TATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGA 630 0.11681766431424323 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 630 0.11681766431424323 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 627 0.11626138972227064 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 620 0.1149634156743346 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 612 0.1134800167624077 No Hit ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG 608 0.11273831730644426 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 608 0.11273831730644426 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 602 0.11162576812249908 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 586 0.1086589702986453 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 575 0.1066192967947458 No Hit GGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATAC 555 0.10291079951492854 No Hit AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG 555 0.10291079951492854 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 550 0.10198367519497424 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 545 0.10105655087501993 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.271243199543113E-5 0.0 12-13 0.0 0.0 0.0 3.708497279817245E-4 0.0 14-15 0.0 0.0 0.0 0.0010198367519497426 0.0 16-17 0.0 0.0 0.0 0.0018542486399086226 0.0 18-19 0.0 0.0 0.0 0.0023178107998857783 0.0 20-21 0.0 0.0 0.0 0.0032449351198400892 0.0 22-23 0.0 0.0 0.0 0.006304445375689317 0.0 24-25 0.0 0.0 0.0 0.010940066975460874 0.0 26-27 0.0 0.0 0.0 0.018357061535095365 0.0 28-29 0.0 0.0 0.0 0.029018991214569946 0.0 30-31 0.0 0.0 0.0 0.06146834241297083 0.0 32-33 0.0 0.0 0.0 0.12006259943408332 0.0 34-35 0.0 0.0 0.0 0.20507989957389366 0.0 36-37 0.0 0.0 0.0 0.3308906697916937 0.0 38-39 0.0 0.0 0.0 0.49888559656741494 0.0 40-41 0.0 0.0 0.0 0.6906149059339666 0.0 42-43 0.0 0.0 0.0 0.8587952575736786 0.0 44-45 0.0 0.0 0.0 1.035412440524975 0.0 46-47 0.0 0.0 0.0 1.2644121475536898 0.0 48-49 0.0 0.0 0.0 1.5076895691097012 0.0 50-51 0.0 0.0 0.0 1.7430864339461007 0.0 52-53 0.0 0.0 0.0 1.9488153205439624 0.0 54-55 0.0 0.0 0.0 2.164186300069349 0.0 56-57 0.0 0.0 0.0 2.402828100025588 0.0 58-59 0.0 0.0 0.0 2.6450856848296502 0.0 60-61 0.0 0.0 0.0 2.9134881754564232 0.0 62-63 0.0 0.0 0.0 3.1713214488357173 0.0 64-65 0.0 0.0 0.0 3.439909364326481 0.0 66-67 0.0 0.0 0.0 3.744840553159454 0.0 68-69 0.0 0.0 0.0 4.094180996918239 0.0 70-71 0.0 0.0 0.0 4.492380892338615 0.0 72-73 0.0 0.0 0.0 4.834860616129738 0.0 74-75 0.0 0.0 0.0 5.202372696559626 0.0 76-77 0.0 0.0 0.0 5.618002529195145 0.0 78-79 0.0 0.0 0.0 6.096676815587556 0.0 80-81 0.0 0.0 0.0 6.601217870506693 0.0 82-83 0.0 0.0 0.0 7.08462049093087 0.0 84-85 0.0 0.0 0.0 7.585082198842207 0.0 86-87 0.0 0.0 0.0 8.11224508716823 0.0 88-89 0.0 0.0 0.0 8.693459323347586 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 75 3.8198777E-11 56.94034 2 GTATGGT 55 8.090119E-7 51.903564 1 TACAGGG 70 7.975905E-8 47.45028 2 GGAGTGA 75 1.3762474E-7 44.270508 6 GTACAGG 100 6.2573235E-10 42.82044 1 GTGGTAC 120 8.54925E-11 39.648552 1 GAGTGCA 60 8.3544146E-5 39.527233 9 AGAGTGC 60 8.3544146E-5 39.527233 8 AATAGCA 50 0.0016263463 37.946148 5 ACCTGGG 100 3.0662704E-8 37.94263 3 CTTACTA 405 0.0 37.477676 9 TGGGTAT 155 1.8189894E-12 36.72208 6 TGGTACT 130 2.0736479E-10 36.500217 2 AGGCTTA 410 0.0 35.863735 6 ATGGGCG 80 1.0870621E-5 35.574512 5 AAGAGTG 80 1.0870621E-5 35.574512 7 GGTATGG 120 3.8380676E-9 35.574512 8 GGGATGC 120 3.8380676E-9 35.574512 7 ATGGTGG 80 1.0877531E-5 35.571213 3 CATGGGT 270 0.0 35.132065 4 >>END_MODULE