FastQCFastQC Report
Thu 26 May 2016
SRR936347_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936347_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539888
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51510.33450484710576356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37210.2416409505106865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25920.16832393005205573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC500.001644952637.862829
GGTATCA35950.037.2669031
GTATCAA58800.035.9886861
ATCAACG69900.030.0627523
TCAACGC71000.029.663654
GTGTACG1601.9772415E-929.5880151
CAACGCA72500.029.0499215
TATCAAC73350.028.6468962
GTACATG78650.028.4707071
AACGCAG75650.027.965436
TACATGG79400.027.2389552
ACATGGG80700.026.5086633
GTATAGG4450.024.4682881
ATAACGC1551.4562975E-624.4276243
ACGCAGA86500.024.3481987
CGCAGAG88750.023.7316918
CATGGGG32850.021.7552724
TATAACG1100.002939937621.5115662
GCAGAGT94250.021.4422059
GTACCTG14250.021.2618431
Produced by FastQC (version 0.11.5)