##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936347_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1539888 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66977078852488 33.0 31.0 34.0 30.0 34.0 2 31.753426223205842 33.0 31.0 34.0 30.0 34.0 3 31.825578873268704 34.0 31.0 34.0 30.0 34.0 4 35.31454040813358 37.0 35.0 37.0 33.0 37.0 5 35.308209428218156 37.0 35.0 37.0 33.0 37.0 6 35.40094474403333 37.0 35.0 37.0 33.0 37.0 7 35.34595632929148 37.0 35.0 37.0 33.0 37.0 8 35.34285805201417 37.0 35.0 37.0 33.0 37.0 9 37.002952162754696 39.0 37.0 39.0 33.0 39.0 10-11 36.92006821275314 39.0 37.0 39.0 33.0 39.0 12-13 36.82473757831738 39.0 37.0 39.0 33.0 39.0 14-15 38.04798173633407 40.0 38.0 41.0 33.0 41.0 16-17 38.00034353147761 40.0 38.0 41.0 33.0 41.0 18-19 37.963345386157954 40.0 38.0 41.0 33.0 41.0 20-21 37.891354436166786 40.0 38.0 41.0 33.0 41.0 22-23 37.731924010057874 40.0 38.0 41.0 32.5 41.0 24-25 37.887942175015326 40.0 38.0 41.0 33.0 41.0 26-27 37.83771806780753 40.0 38.0 41.0 33.0 41.0 28-29 37.700908117992995 40.0 38.0 41.0 32.5 41.0 30-31 37.53534672651517 40.0 37.5 41.0 32.0 41.0 32-33 37.33922694377773 40.0 37.0 41.0 32.0 41.0 34-35 37.07679714368837 40.0 37.0 41.0 31.0 41.0 36-37 36.87743199505418 40.0 37.0 41.0 30.5 41.0 38-39 36.71359053385701 40.0 36.0 41.0 30.0 41.0 40-41 36.527907873819395 40.0 36.0 41.0 30.0 41.0 42-43 36.33605236224972 40.0 36.0 41.0 30.0 41.0 44-45 36.14278863138098 40.0 35.0 41.0 29.5 41.0 46-47 35.847809061438234 39.0 35.0 41.0 28.0 41.0 48-49 35.66181176812859 39.0 35.0 41.0 27.5 41.0 50-51 35.354812492856624 39.0 35.0 40.5 27.0 41.0 52-53 35.37330182454828 39.0 35.0 40.5 27.0 41.0 54-55 35.45886129380838 39.0 35.0 41.0 27.0 41.0 56-57 35.31528169581164 39.0 35.0 41.0 26.5 41.0 58-59 35.09776068129631 39.0 35.0 41.0 26.0 41.0 60-61 34.81975864478456 38.5 35.0 40.5 26.0 41.0 62-63 34.523948819654414 38.0 34.0 40.0 26.0 41.0 64-65 34.20625688361751 37.0 34.0 40.0 25.0 41.0 66-67 33.86036809170537 37.0 34.0 40.0 23.5 41.0 68-69 33.48940020313165 36.0 34.0 39.0 23.0 41.0 70-71 33.08740700622383 36.0 33.0 39.0 22.0 41.0 72-73 32.687317843895144 35.0 33.0 38.5 21.5 40.0 74-75 32.26930042964163 35.0 33.0 37.5 20.0 39.5 76-77 31.862535781823095 35.0 32.0 37.0 18.5 39.0 78-79 31.461772869195684 35.0 32.0 36.5 17.5 39.0 80-81 31.061530124268778 35.0 32.0 36.0 13.5 38.0 82-83 30.712124842845714 35.0 31.0 35.5 8.5 37.0 84-85 30.386403751441662 35.0 31.0 35.0 7.0 37.0 86-87 30.054672807373002 34.5 31.0 35.0 2.0 36.0 88-89 29.7267057734069 34.0 31.0 35.0 2.0 36.0 90-91 29.377460568560828 34.0 30.0 35.0 2.0 36.0 92-93 29.07346410907806 34.0 30.0 35.0 2.0 35.0 94-95 28.705245121723138 34.0 29.5 35.0 2.0 35.0 96-97 28.294650325218456 34.0 29.0 35.0 2.0 35.0 98-99 27.81252305362468 34.0 29.0 35.0 2.0 35.0 100-101 24.590350402107166 30.0 22.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11191741919002496 1101 2 0.0895521414499072 1101 3 0.15010739237604653 1101 4 -0.0932612404922537 1101 5 0.15621767670588582 1101 6 0.20145066144685586 1101 7 0.24181729340268987 1101 8 0.24066414395860392 1101 9 0.1362481537603628 1101 10-11 0.33405313036322326 1101 12-13 0.206713152315821 1101 14-15 0.35544745054329496 1101 16-17 0.4015491245084988 1101 18-19 0.34297743783374557 1101 20-21 0.4519114091607932 1101 22-23 0.4862590392506547 1101 24-25 0.4215358151090953 1101 26-27 0.37500784529090225 1101 28-29 0.43460792236224677 1101 30-31 0.5056172954487437 1101 32-33 0.4600956727257284 1101 34-35 0.33500614800613704 1101 36-37 0.3380532843798534 1101 38-39 0.537645059647879 1101 40-41 0.3372008537501259 1101 42-43 0.45441205124200224 1101 44-45 0.5443369841107213 1101 46-47 0.29035096461099386 1101 48-49 0.415381882226292 1101 50-51 0.49764034914461774 1101 52-53 0.5846718597261713 1101 54-55 0.4830977604344824 1101 56-57 0.5033462868832856 1101 58-59 0.46679241300715546 1101 60-61 0.3157434742833871 1101 62-63 0.38502914155406387 1101 64-65 0.2709871106260735 1101 66-67 0.48357338982689413 1101 68-69 0.46525372363954176 1101 70-71 0.5668831807208576 1101 72-73 0.5440208637853559 1101 74-75 0.31333829489467746 1101 76-77 0.31015349117618385 1101 78-79 0.3507269024088835 1101 80-81 0.3063559439810071 1101 82-83 0.3497338876112117 1101 84-85 0.24361102813891122 1101 86-87 0.28658517130781647 1101 88-89 0.1354603273615318 1101 90-91 0.21181792787807652 1101 92-93 0.23179491674522623 1101 94-95 0.41257226735160657 1101 96-97 0.4245949706903467 1101 98-99 0.46005302198583564 1101 100-101 0.7923068922334195 1102 1 0.11035767247834016 1102 2 0.22302871682819259 1102 3 0.2575355155642036 1102 4 0.1355071052894985 1102 5 0.16215881104646002 1102 6 0.15069181966110534 1102 7 0.25131617361368797 1102 8 0.16179234558882882 1102 9 0.1592009862012702 1102 10-11 0.2262578073296666 1102 12-13 0.3098602141486495 1102 14-15 0.34297260316428435 1102 16-17 0.34725243158079167 1102 18-19 0.2992636036079901 1102 20-21 0.3492307366052856 1102 22-23 0.34204982628610736 1102 24-25 0.24883308782603564 1102 26-27 0.2825519263249703 1102 28-29 0.2948269917436903 1102 30-31 0.42664716027827865 1102 32-33 0.27857247547233044 1102 34-35 0.23707150503125263 1102 36-37 0.4028464360458983 1102 38-39 0.360610113136282 1102 40-41 0.35717260323570343 1102 42-43 0.28986303631527477 1102 44-45 0.14576798350820042 1102 46-47 0.42942250605455 1102 48-49 0.43224948565045906 1102 50-51 0.464893910301015 1102 52-53 0.6056795561439898 1102 54-55 0.45361775179448927 1102 56-57 0.5340350423827616 1102 58-59 0.4257373671355893 1102 60-61 0.4079538354579668 1102 62-63 0.5067902797598975 1102 64-65 0.33428673660517916 1102 66-67 0.3264740980792311 1102 68-69 0.23703305131140695 1102 70-71 0.43203330065764334 1102 72-73 0.4509482082370013 1102 74-75 0.4792344671489204 1102 76-77 0.27533203215364566 1102 78-79 0.37061395719327805 1102 80-81 0.48207243841616787 1102 82-83 0.4985049618128663 1102 84-85 0.542414502326924 1102 86-87 0.5141601175920449 1102 88-89 0.4936626396974013 1102 90-91 0.47869633129769795 1102 92-93 0.5193609956647727 1102 94-95 0.4677903704176707 1102 96-97 0.541002153255743 1102 98-99 0.3524586357097874 1102 100-101 0.31705252391946814 1103 1 0.32713823228590755 1103 2 0.2751115945889602 1103 3 0.3238459244861396 1103 4 0.2912391192846826 1103 5 0.23069150293362384 1103 6 0.1631344175180942 1103 7 0.27370027595350876 1103 8 0.2351932858906025 1103 9 0.23917583859576297 1103 10-11 0.2806408709640493 1103 12-13 0.29451334119871575 1103 14-15 0.37126639279910023 1103 16-17 0.34387800560222814 1103 18-19 0.36379727953188734 1103 20-21 0.45578688136802015 1103 22-23 0.4286081577971501 1103 24-25 0.40272959996248403 1103 26-27 0.39923544917649423 1103 28-29 0.4836882425637157 1103 30-31 0.5383940988306506 1103 32-33 0.5265059983238558 1103 34-35 0.43103061283794375 1103 36-37 0.38728088826544393 1103 38-39 0.4300571391585848 1103 40-41 0.4058181860041188 1103 42-43 0.1577793385238877 1103 44-45 0.2572361116516433 1103 46-47 0.35272504268259297 1103 48-49 0.2924629669537566 1103 50-51 0.32312155079959837 1103 52-53 0.5249297748188226 1103 54-55 0.5184548390457451 1103 56-57 0.3462193305961776 1103 58-59 0.46139913184148185 1103 60-61 0.39389714400815734 1103 62-63 0.4790568443783201 1103 64-65 0.4200168371955968 1103 66-67 0.4271738575369213 1103 68-69 0.4104394584839426 1103 70-71 0.3295540747665413 1103 72-73 0.3462863341937066 1103 74-75 0.4260226805755565 1103 76-77 0.37440403084159257 1103 78-79 0.3803354201278957 1103 80-81 0.2729618982893349 1103 82-83 0.28606236395587814 1103 84-85 0.4500858887253507 1103 86-87 0.31499518210112143 1103 88-89 0.28141958262327194 1103 90-91 0.3348301602939756 1103 92-93 0.3415797251640065 1103 94-95 0.362889320778482 1103 96-97 0.48454167873136456 1103 98-99 0.27698165123570107 1103 100-101 0.34627568158402156 1104 1 0.16912650158206333 1104 2 0.07387052012449402 1104 3 0.233166733734695 1104 4 0.16315768439859113 1104 5 0.1952018735558667 1104 6 0.17699024886797332 1104 7 0.2694882732333497 1104 8 0.2562618000014467 1104 9 0.29763384607620935 1104 10-11 0.27871338031460624 1104 12-13 0.2784537526234061 1104 14-15 0.42010182292490583 1104 16-17 0.4824894472887209 1104 18-19 0.2773640935407329 1104 20-21 0.4139579966349416 1104 22-23 0.47071330910623743 1104 24-25 0.5177478760590475 1104 26-27 0.4839196552288598 1104 28-29 0.5069031354572928 1104 30-31 0.3277578356548574 1104 32-33 0.4156338459947122 1104 34-35 0.3152069134159774 1104 36-37 0.22225411165884879 1104 38-39 0.22922621430309675 1104 40-41 0.27061969909490813 1104 42-43 0.4187301205708067 1104 44-45 0.3700825074353915 1104 46-47 0.4183659811388978 1104 48-49 0.28681225291091295 1104 50-51 0.2231258010546071 1104 52-53 0.26237664663112525 1104 54-55 0.3361784024595451 1104 56-57 0.4580116631361406 1104 58-59 0.4485697370777899 1104 60-61 0.44227142146480247 1104 62-63 0.6198932945653297 1104 64-65 0.486773467593423 1104 66-67 0.5497214603930871 1104 68-69 0.44394421876955903 1104 70-71 0.4218696136988811 1104 72-73 0.4421444606812983 1104 74-75 0.40614423786899323 1104 76-77 0.49066338140263 1104 78-79 0.4906826659626482 1104 80-81 0.4077119832944369 1104 82-83 0.43058803549617153 1104 84-85 0.39488995196914445 1104 86-87 0.2344294731585883 1104 88-89 0.38413124532303655 1104 90-91 0.26459894642114534 1104 92-93 0.10445162247784268 1104 94-95 0.026439133767262035 1104 96-97 0.3786869524477865 1104 98-99 0.6817313112152981 1104 100-101 0.6706168920566498 1105 1 0.2609977847524263 1105 2 0.2983316318834177 1105 3 0.2228012558982435 1105 4 0.15016647985427056 1105 5 0.1511017419571914 1105 6 0.17084758924211485 1105 7 0.2704815100609892 1105 8 0.3279060338139814 1105 9 0.2915703122353648 1105 10-11 0.31075932324409195 1105 12-13 0.3168974317225377 1105 14-15 0.3983578489781081 1105 16-17 0.45787508137777877 1105 18-19 0.45987500457002994 1105 20-21 0.4352251336141677 1105 22-23 0.5451925365054109 1105 24-25 0.46628592306809225 1105 26-27 0.4712720231720908 1105 28-29 0.4740107548539356 1105 30-31 0.46735133734565437 1105 32-33 0.48350294695922 1105 34-35 0.3938548203307377 1105 36-37 0.38280449396132354 1105 38-39 0.5411702515505183 1105 40-41 0.478488341962759 1105 42-43 0.517900757408448 1105 44-45 0.5283403242300508 1105 46-47 0.4217007165256135 1105 48-49 0.3313985916359101 1105 50-51 0.42479556334409097 1105 52-53 0.48655144798376426 1105 54-55 0.4914502619987857 1105 56-57 0.6684508528880926 1105 58-59 0.6892099896139783 1105 60-61 0.5255794206652169 1105 62-63 0.4268742710608038 1105 64-65 0.4539071152088212 1105 66-67 0.42546170293444874 1105 68-69 0.432004554262889 1105 70-71 0.5307852156378843 1105 72-73 0.5233004467551581 1105 74-75 0.6100305632675358 1105 76-77 0.7379751869697522 1105 78-79 0.5871130519298937 1105 80-81 0.5505746292606908 1105 82-83 0.5491705178802739 1105 84-85 0.6968152072539127 1105 86-87 0.6706603372121585 1105 88-89 0.7736831018638206 1105 90-91 0.7859108519366274 1105 92-93 0.5813375230958577 1105 94-95 0.5027753184051988 1105 96-97 0.6505454505570967 1105 98-99 0.6126222259093694 1105 100-101 0.8810040253711016 1106 1 0.15640048036062382 1106 2 0.037085214120338605 1106 3 0.15027350108781334 1106 4 0.15799522284031298 1106 5 0.13340328402489376 1106 6 0.08657370966949429 1106 7 0.08419716273050426 1106 8 -0.025946727217700527 1106 9 0.0424733971735094 1106 10-11 0.11836309773776321 1106 12-13 -0.005196842317580774 1106 14-15 0.11275623386086409 1106 16-17 0.13489779095369414 1106 18-19 0.025432950513376795 1106 20-21 0.04887736965248024 1106 22-23 0.0684871107710947 1106 24-25 0.10056160881058673 1106 26-27 0.04087378278291709 1106 28-29 0.12353954520176558 1106 30-31 -0.029258080425456967 1106 32-33 0.0887745547715042 1106 34-35 -0.06527857936261228 1106 36-37 0.12717827479665544 1106 38-39 0.0232500360945167 1106 40-41 0.07374072410014065 1106 42-43 0.11576004473394619 1106 44-45 0.09821902988857545 1106 46-47 0.2590149820566694 1106 48-49 0.35541663727982353 1106 50-51 0.25824362191215755 1106 52-53 0.23626948479751775 1106 54-55 0.3137030776169425 1106 56-57 0.2962183065096653 1106 58-59 0.3163362529294673 1106 60-61 0.3508435228382396 1106 62-63 0.4246737540948402 1106 64-65 0.2437090787161651 1106 66-67 0.23659954981939535 1106 68-69 0.3555463731160913 1106 70-71 0.361474851433762 1106 72-73 0.390876085135865 1106 74-75 0.38485235450640687 1106 76-77 0.4930966819967644 1106 78-79 0.5258427525873408 1106 80-81 0.3382924734163275 1106 82-83 0.25036680439500714 1106 84-85 0.23781426001815476 1106 86-87 0.231191110333139 1106 88-89 0.3664249078731494 1106 90-91 0.37640397644996426 1106 92-93 0.3282881062880456 1106 94-95 0.5546495206801687 1106 96-97 0.3438086376040488 1106 98-99 0.3631349774686967 1106 100-101 0.41102457557058614 1107 1 0.027033190269097673 1107 2 0.13744021024574948 1107 3 0.19875193952373493 1107 4 0.10708940676809675 1107 5 0.11989981783853665 1107 6 0.18533698275471266 1107 7 0.06301363287522577 1107 8 0.1544049179118332 1107 9 0.2150161747302164 1107 10-11 0.16538490015467033 1107 12-13 0.26953821957112467 1107 14-15 0.24749925436477582 1107 16-17 -0.05080078696743584 1107 18-19 0.07575548386625996 1107 20-21 0.21282746075767278 1107 22-23 0.30541875045526723 1107 24-25 0.2809389100022912 1107 26-27 0.2929356924316906 1107 28-29 0.313359848048016 1107 30-31 0.28531194491645095 1107 32-33 0.2727908280452169 1107 34-35 0.37633136471764317 1107 36-37 0.3420713570845564 1107 38-39 0.31704022186535497 1107 40-41 0.34840719529204733 1107 42-43 0.08470050172601162 1107 44-45 0.4896802735269219 1107 46-47 0.46010562649506426 1107 48-49 0.1733093844681335 1107 50-51 0.3685107591144998 1107 52-53 0.26757406937875317 1107 54-55 0.503483587387187 1107 56-57 0.21593682285624283 1107 58-59 0.3546847355566456 1107 60-61 0.2550343620532658 1107 62-63 0.42424702571400985 1107 64-65 0.1849687390089514 1107 66-67 0.2118487801375366 1107 68-69 0.3315197326988937 1107 70-71 0.2563729602154652 1107 72-73 0.13090320725871862 1107 74-75 0.05695479026153549 1107 76-77 0.05109299590130334 1107 78-79 0.03924464171126729 1107 80-81 0.17898246441052024 1107 82-83 0.3491981445839194 1107 84-85 0.1740437613480843 1107 86-87 0.12834381102637948 1107 88-89 0.24866305896117424 1107 90-91 0.479942942248865 1107 92-93 0.21581402414675566 1107 94-95 0.171298210650221 1107 96-97 0.3408414354761682 1107 98-99 0.37813434915697286 1107 100-101 0.6446889942775194 1108 1 -0.05824761119511379 1108 2 -0.06661324774176691 1108 3 -0.0753870492158164 1108 4 -0.004938640423375773 1108 5 -0.034549859963519225 1108 6 -0.08701432707751877 1108 7 -0.08174526369187873 1108 8 -0.0278283118246776 1108 9 0.05484856082019007 1108 10-11 -0.06366741708482238 1108 12-13 -0.17322817044302496 1108 14-15 -0.10954803344341713 1108 16-17 0.04143933795715071 1108 18-19 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-1.4945509097404255 2316 48-49 -1.6053418231304377 2316 50-51 -1.550344562372402 2316 52-53 -1.5638321488695794 2316 54-55 -1.5567586096971198 2316 56-57 -1.4931880915318274 2316 58-59 -1.7110494196511965 2316 60-61 -1.6981511542901444 2316 62-63 -1.622722876519056 2316 64-65 -1.3474053644177815 2316 66-67 -1.4989699333127575 2316 68-69 -1.5190643099904193 2316 70-71 -1.2357428397950727 2316 72-73 -1.224681324641601 2316 74-75 -1.2657212692566304 2316 76-77 -1.6153132599786346 2316 78-79 -1.4979233533514673 2316 80-81 -1.2968527693194467 2316 82-83 -1.334578865508881 2316 84-85 -1.5656500678194298 2316 86-87 -1.7991163559074757 2316 88-89 -1.7157739344231864 2316 90-91 -1.8080126825488634 2316 92-93 -1.7244491713795966 2316 94-95 -1.7150599520237328 2316 96-97 -2.0137665559739304 2316 98-99 -1.9443915602110415 2316 100-101 -1.8356834834633808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14079.0 3 2975.0 4 2387.0 5 2033.0 6 1922.0 7 2126.0 8 2607.0 9 2941.0 10 3512.0 11 4427.0 12 5771.0 13 7982.0 14 8486.0 15 7633.0 16 9023.0 17 11653.0 18 10676.0 19 7441.0 20 7152.0 21 7255.0 22 7717.0 23 8357.0 24 9610.0 25 11049.0 26 13194.0 27 15843.0 28 18703.0 29 22652.0 30 27846.0 31 34603.0 32 44055.0 33 57189.0 34 79965.0 35 119870.0 36 195183.0 37 327866.0 38 353408.0 39 70577.0 40 120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.710250049703276 17.944766342631635 12.670371780494746 23.674611827170345 2 15.691572274758393 19.23263275485815 40.60343707783435 24.472357892549102 3 20.242500841069656 24.123891184420923 27.955012190638996 27.67859578387043 4 12.480451905192389 15.11970447956607 36.67735642216611 35.72248719307543 5 13.830877501078106 37.79531036489372 33.5798474543688 14.793964679659371 6 33.48592960222575 34.967297552333335 17.249331565996208 14.297441279444707 7 30.30953249954865 31.775691223439832 19.727874810203364 18.186901466808155 8 27.378683905963225 34.980561628017234 19.081622977415638 18.559131488603906 9 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28.5001706525096 22.66749194718248 34-35 22.754644829913392 26.624888780948037 28.252097168907436 22.36836922023114 36-37 22.931114630617643 26.436140801283987 28.10072240499371 22.532022163104664 38-39 22.588018388683906 26.52733690611992 28.27128038698048 22.613364318215698 40-41 22.728398360686224 26.467458916827436 28.39333611968387 22.41080660280247 42-43 22.684212518073483 26.465746506663045 28.149810261041136 22.70023071422233 44-45 22.681731982766834 26.68409690980257 28.02108987636339 22.613081231067206 46-47 22.729599919596915 26.840338202005576 27.695240979207775 22.734820899189735 48-49 22.902469023397945 26.83944837515815 27.564664238076265 22.69341836336764 50-51 22.87830405318078 26.893592744562273 27.43602731624784 22.792075886009105 52-53 22.995646409193153 26.99598318347639 27.34352641181686 22.664843995513593 54-55 22.824155117422976 26.875708657242374 27.48361625241657 22.81651997291808 56-57 22.86779239406419 27.230415651687057 27.253254732094323 22.64853722215443 58-59 22.96516152018305 26.84842574469661 27.322589791166184 22.86382294395416 60-61 22.968561026609084 26.892600880694506 27.49477093753803 22.64406715515838 62-63 22.841323501488674 26.924853726189518 27.518986069813405 22.714836702508396 64-65 22.737212987229956 26.908230309801507 27.493154242933667 22.861402460034874 66-67 22.831750613194636 27.0930741219304 27.26913005793829 22.806045206936677 68-69 22.791119742899742 26.97448086089039 27.42709504963224 22.807304346577624 70-71 22.680583897313937 26.883842016921868 27.496937458914644 22.938636626849554 72-73 22.73782447591029 27.06489860785434 27.25520973865743 22.942067177577936 74-75 22.71217633910362 27.1397105610938 27.199425690558154 22.948687409244425 76-77 22.751815608145122 27.072602465749046 27.251104503791414 22.924477422314418 78-79 22.80238280745303 27.039017852132297 27.27415989006041 22.884439450354265 80-81 22.659270613707953 27.24819715499279 27.290286921698026 22.80224530960123 82-83 22.80211193021312 27.360211219123524 27.108194333600665 22.729482517062692 84-85 22.84799683641464 27.07031962457221 27.09837947819766 22.98330406081549 86-87 22.733212586355243 26.9659368881148 27.28794695659863 23.012903568931332 88-89 22.911045583476593 27.009329203264894 27.14485523222498 22.934769981033533 90-91 22.811384067006575 27.195209526109977 27.257456962116215 22.735949444767233 92-93 22.83676402097545 27.00499320651065 27.215635436850903 22.942607335662995 94-95 22.73577659927815 27.07381022762792 27.254402570512582 22.936010602581344 96-97 22.792165811734975 27.03648904571879 27.122014833603085 23.04933030894315 98-99 22.783086868317884 26.87402560974814 27.03759270976706 23.305294812166913 100-101 22.90258974572249 27.110310870604042 26.789834118623695 23.19726526504977 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 405.0 1 267.0 2 369.0 3 570.5 4 418.5 5 419.0 6 921.5 7 1676.0 8 2259.5 9 2349.0 10 1898.5 11 1549.0 12 1603.5 13 1716.5 14 1807.5 15 1905.0 16 2030.0 17 2100.0 18 2113.5 19 2177.5 20 2287.5 21 2462.0 22 2748.0 23 3304.0 24 3928.5 25 4616.5 26 6076.5 27 8085.0 28 9804.0 29 11896.5 30 14373.0 31 16868.5 32 20331.0 33 23893.0 34 27629.5 35 30865.5 36 34118.5 37 37600.5 38 40017.5 39 42557.0 40 44726.5 41 47474.5 42 50205.0 43 52792.0 44 55463.5 45 57519.0 46 58693.5 47 58626.0 48 60336.0 49 60413.0 50 58004.5 51 55558.5 52 54021.0 53 53730.0 54 52086.0 55 48824.5 56 44651.0 57 40684.5 58 37572.5 59 33973.0 60 29458.5 61 24793.0 62 20511.5 63 16369.5 64 12562.0 65 9770.0 66 7186.0 67 5503.5 68 4058.5 69 2672.0 70 2007.0 71 1583.0 72 1368.0 73 1305.5 74 1054.5 75 806.5 76 577.5 77 336.5 78 212.5 79 135.5 80 96.0 81 70.0 82 49.5 83 31.0 84 21.5 85 19.0 86 16.0 87 14.0 88 9.5 89 8.5 90 12.0 91 14.0 92 13.0 93 9.0 94 7.0 95 11.0 96 13.5 97 13.5 98 8.0 99 4.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0489646000228588 2 0.013507475868374842 3 0.011819041384827988 4 0.0062342196315576205 5 0.009870847749966232 6 0.006169279843728895 7 0.005325062601955467 8 0.00974096817430878 9 0.014286753322319544 10-11 0.0021754828922622943 12-13 0.008799341250792266 14-15 0.009026630508192805 16-17 0.020131334226904815 18-19 0.01298795756574504 20-21 0.003539218436665524 22-23 0.010065667113452408 24-25 0.02256657627048201 26-27 0.008344762735991188 28-29 0.018377959955529235 30-31 0.015585549078894048 32-33 0.013604885550117931 34-35 0.26414258699333976 36-37 0.46500135074758686 38-39 0.46091014411437714 40-41 0.4816908762195692 42-43 0.4712031004852301 44-45 0.4866587699884667 46-47 0.49409437569485576 48-49 0.5015624512951592 50-51 0.5134139625739015 52-53 0.5003285953264133 54-55 0.4870808786093534 56-57 0.4823078042039421 58-59 0.4806518396143096 60-61 0.47714509107155845 62-63 0.4751644275427823 64-65 0.49042527768253275 66-67 0.46344279583969744 68-69 0.491431844393878 70-71 0.4711706305913157 72-73 0.48039208046299475 74-75 0.49438660474008495 76-77 0.4813661772804256 78-79 0.4812038278108538 80-81 0.48373647953617405 82-83 0.4838663591118315 84-85 0.4837689494300884 86-87 0.4859119624284363 88-89 0.5006857641594713 90-91 0.5258824018370167 92-93 0.5134464324678158 94-95 0.482567563355257 96-97 0.4556824911941647 98-99 0.44665586068597196 100-101 0.45558508151242166 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1539888.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.92228000068718 #Duplication Level Percentage of deduplicated Percentage of total 1 72.67204469264118 39.1864234213905 2 14.420370857282542 15.551585501602771 3 5.5322631608700465 8.94936729593764 4 2.54655212602767 5.4926358710403695 5 1.4368333475399724 3.8738665040187534 6 0.8633764680025003 2.79331365921811 7 0.5550051345630646 2.094899958741004 8 0.3741776472099646 1.6141209490283248 9 0.2674077347526677 1.2977311272914553 >10 1.2100122514844909 11.479382473854582 >50 0.08533458549024214 3.1624291192062364 >100 0.03469461620461999 3.1846105619147664 >500 0.0014455334482637295 0.5080160487982155 >1k 3.6138336206593236E-4 0.4770323312075304 >5k 1.2046112068864412E-4 0.33458517674972615 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5151 0.33450484710576356 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3721 0.2416409505106865 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2592 0.16832393005205573 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 6.49397878287252E-5 0.0 0.0 0.0 12-13 0.0 6.49397878287252E-5 0.0 0.0 0.0 14-15 0.0 6.49397878287252E-5 0.0 0.0 0.0 16-17 0.0 6.49397878287252E-5 0.0 9.74096817430878E-5 0.0 18-19 0.0 6.49397878287252E-5 0.0 2.272892574005382E-4 0.0 20-21 0.0 6.49397878287252E-5 0.0 4.545785148010764E-4 0.0 22-23 0.0 6.49397878287252E-5 0.0 0.0011689161809170538 0.0 24-25 0.0 6.49397878287252E-5 0.0 0.0017533742713755805 0.0 26-27 0.0 6.49397878287252E-5 0.0 0.0018507839531186686 0.0 28-29 0.0 6.49397878287252E-5 0.0 0.0025001818314059204 0.0 30-31 0.0 6.49397878287252E-5 0.0 0.004805544299325665 0.0 32-33 0.0 6.49397878287252E-5 0.0 0.008409702523819914 0.0 34-35 0.0 6.49397878287252E-5 0.0 0.01285807799008759 0.0 36-37 0.0 6.49397878287252E-5 0.0 0.021300250407821866 0.0 38-39 0.0 6.49397878287252E-5 0.0 0.03477525638228235 0.0 40-41 0.0 6.49397878287252E-5 0.0 0.05198430015689452 0.0 42-43 0.0 6.49397878287252E-5 0.0 0.0678945481749322 0.0 44-45 0.0 6.49397878287252E-5 0.0 0.0873440146296354 0.0 46-47 0.0 6.49397878287252E-5 0.0 0.11127432644452065 0.0 48-49 0.0 6.49397878287252E-5 0.0 0.13802951902995542 0.0 50-51 0.0 6.49397878287252E-5 0.0 0.1689408580364286 0.0 52-53 0.0 9.74096817430878E-5 0.0 0.19738448510541026 0.0 54-55 0.0 1.298795756574504E-4 0.0 0.22975696933802978 0.0 56-57 0.0 1.298795756574504E-4 0.0 0.2670324075517181 0.0 58-59 0.0 1.298795756574504E-4 0.0 0.3108018245482788 0.0 60-61 0.0 1.298795756574504E-4 0.0 0.3586624481780493 0.0 62-63 0.0 1.298795756574504E-4 0.0 0.410127230032314 0.0 64-65 0.0 1.948193634861756E-4 0.0 0.46289080764315327 0.0 66-67 0.0 1.948193634861756E-4 0.0 0.5224405930820943 0.0 68-69 0.0 1.948193634861756E-4 0.0 0.5952705651320096 0.0 70-71 0.0 1.948193634861756E-4 0.0 0.6764128300240018 0.0 72-73 0.0 2.597591513149008E-4 0.0 0.7579772035368806 0.0 74-75 0.0 2.597591513149008E-4 0.0 0.8384051307627567 0.0 76-77 0.0 2.597591513149008E-4 0.0 0.9327626424778945 0.0 78-79 0.0 2.597591513149008E-4 0.0 1.0470241991625364 0.0 80-81 0.0 2.597591513149008E-4 0.0 1.1784623297278762 0.0 82-83 0.0 2.597591513149008E-4 0.0 1.3124655819124507 0.0 84-85 0.0 2.597591513149008E-4 0.0 1.4462415448396246 0.0 86-87 0.0 2.597591513149008E-4 0.0 1.5901481146680796 0.0 88-89 0.0 2.597591513149008E-4 0.0 1.750354571241545 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCGAC 50 0.0016449526 37.86282 9 GGTATCA 3595 0.0 37.266903 1 GTATCAA 5880 0.0 35.988686 1 ATCAACG 6990 0.0 30.062752 3 TCAACGC 7100 0.0 29.66365 4 GTGTACG 160 1.9772415E-9 29.588015 1 CAACGCA 7250 0.0 29.049921 5 TATCAAC 7335 0.0 28.646896 2 GTACATG 7865 0.0 28.470707 1 AACGCAG 7565 0.0 27.96543 6 TACATGG 7940 0.0 27.238955 2 ACATGGG 8070 0.0 26.508663 3 GTATAGG 445 0.0 24.468288 1 ATAACGC 155 1.4562975E-6 24.427624 3 ACGCAGA 8650 0.0 24.348198 7 CGCAGAG 8875 0.0 23.731691 8 CATGGGG 3285 0.0 21.755272 4 TATAACG 110 0.0029399376 21.511566 2 GCAGAGT 9425 0.0 21.442205 9 GTACCTG 1425 0.0 21.261843 1 >>END_MODULE