FastQCFastQC Report
Thu 26 May 2016
SRR936347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936347_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539888
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55660.3614548590546845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40350.2620320438889062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27890.18111706825431462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59250.040.1407741
GGTATCA34150.038.1648861
ATCAACG72500.032.2540633
TCAACGC72900.032.012024
CAACGCA74300.031.346015
AACGCAG76900.030.594616
TATCAAC76950.030.4573822
TATAACG1303.0699812E-729.195832
GTACATG77050.028.274631
TACATGG80400.026.6130522
ACGCAGA89350.026.331577
CGCAGAG90400.025.8683138
ACATGGG78400.025.8337423
GCAGAGT96600.023.9134069
GTACCTG13650.021.9539171
CATGGGA45650.020.9887264
GAGTACT49600.020.94363412-13
AGTACAT55700.020.783032
AGTACTT52900.020.75797312-13
GTATTCG1150.003693634920.6812251
Produced by FastQC (version 0.11.5)