##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936347_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1539888 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48047974917657 34.0 31.0 34.0 31.0 34.0 2 32.60703505709506 34.0 31.0 34.0 31.0 34.0 3 32.69298156749062 34.0 31.0 34.0 31.0 34.0 4 36.17592772980892 37.0 35.0 37.0 35.0 37.0 5 36.143509787724824 37.0 35.0 37.0 35.0 37.0 6 36.18286524734266 37.0 37.0 37.0 35.0 37.0 7 36.13820420705922 37.0 36.0 37.0 35.0 37.0 8 36.11472133038247 37.0 36.0 37.0 35.0 37.0 9 37.88393311721372 39.0 38.0 39.0 35.0 39.0 10-11 37.816030126866366 39.0 38.0 39.0 35.0 39.0 12-13 37.76080630539364 39.0 38.0 39.0 35.0 39.0 14-15 39.097829192772465 40.0 38.5 41.0 36.0 41.0 16-17 39.03316897073034 40.0 38.0 41.0 35.5 41.0 18-19 39.068855657034796 40.0 38.5 41.0 36.0 41.0 20-21 39.029056658666086 40.0 38.5 41.0 35.0 41.0 22-23 38.91652639672496 40.0 38.0 41.0 35.0 41.0 24-25 39.0605216093638 40.0 39.0 41.0 35.0 41.0 26-27 38.97863903089056 40.0 38.5 41.0 35.0 41.0 28-29 38.88332722899328 40.0 38.0 41.0 35.0 41.0 30-31 38.755535792213465 40.0 38.0 41.0 35.0 41.0 32-33 38.58863177062228 40.0 38.0 41.0 34.5 41.0 34-35 38.451291587440124 40.0 38.0 41.0 34.0 41.0 36-37 38.30435362831582 40.0 38.0 41.0 34.0 41.0 38-39 38.17180307918498 40.0 38.0 41.0 34.0 41.0 40-41 38.02921446235051 40.0 38.0 41.0 33.0 41.0 42-43 38.04051203723907 40.0 38.0 41.0 33.0 41.0 44-45 37.89122163430068 40.0 38.0 41.0 33.0 41.0 46-47 37.66536007813555 40.0 37.0 41.0 33.0 41.0 48-49 37.425246511434594 40.0 37.0 41.0 32.5 41.0 50-51 37.23125577964112 40.0 37.0 41.0 32.0 41.0 52-53 37.02400726546347 40.0 36.0 41.0 31.5 41.0 54-55 36.839902317571145 40.0 36.0 41.0 31.0 41.0 56-57 36.609378734037804 39.0 35.5 41.0 31.0 41.0 58-59 36.36321505200378 39.0 35.0 41.0 31.0 41.0 60-61 36.10002383290214 39.0 35.0 41.0 30.0 41.0 62-63 35.81252467711937 38.0 35.0 40.0 30.0 41.0 64-65 35.45598446120757 37.5 35.0 40.0 29.0 41.0 66-67 35.12260307243124 37.0 34.5 40.0 29.0 41.0 68-69 34.77218343152229 36.5 34.0 39.0 28.5 41.0 70-71 34.40375631214738 36.0 34.0 39.0 28.0 41.0 72-73 33.99682704196669 35.5 34.0 39.0 28.0 40.0 74-75 33.566525617447496 35.0 33.5 37.5 27.0 39.5 76-77 32.82940545026651 35.0 32.5 37.0 26.0 39.0 78-79 32.86655555468969 35.0 33.0 36.5 26.0 39.0 80-81 32.67813892958449 35.0 33.0 36.0 26.0 38.5 82-83 32.3902579278493 35.0 33.0 36.0 26.0 37.0 84-85 32.092572316947724 35.0 33.0 35.0 26.0 37.0 86-87 31.679076984819673 35.0 32.5 35.0 25.0 36.5 88-89 31.18713211610195 35.0 32.0 35.0 23.0 36.0 90-91 30.96494777542263 35.0 32.0 35.0 21.5 36.0 92-93 30.685286202632916 34.0 31.5 35.0 20.0 35.5 94-95 30.528586494602205 34.0 31.0 35.0 19.0 35.0 96-97 30.28606918165477 34.0 31.0 35.0 16.5 35.0 98-99 29.843391857070124 34.0 31.0 35.0 2.0 35.0 100-101 26.56875987084775 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04511753833516963 1101 2 0.027041052397649423 1101 3 0.10444927416079963 1101 4 0.12137879278029828 1101 5 0.04422369536402471 1101 6 0.0430534014568309 1101 7 0.11427772928856683 1101 8 -0.005022441448289783 1101 9 0.08194995963471996 1101 10-11 0.17171652205169607 1101 12-13 0.1703501707888435 1101 14-15 0.29206381830279327 1101 16-17 0.2770380661220315 1101 18-19 0.18526699832010962 1101 20-21 0.21886343977067924 1101 22-23 0.2256850504995569 1101 24-25 0.010077382593685513 1101 26-27 0.203642300448152 1101 28-29 0.18584463570068266 1101 30-31 0.0418897325905192 1101 32-33 0.1823942056692971 1101 34-35 0.15540380436222279 1101 36-37 0.07502787034425751 1101 38-39 0.2056303129930015 1101 40-41 0.20269587921038834 1101 42-43 0.15829233132588882 1101 44-45 0.16968704955552738 1101 46-47 0.2488523665623461 1101 48-49 0.15965157453691603 1101 50-51 0.06141900616164975 1101 52-53 0.2096315495879324 1101 54-55 0.1650154261930723 1101 56-57 0.11906815553166439 1101 58-59 0.11416549399900333 1101 60-61 -0.003022819005586541 1101 62-63 0.1664967969110691 1101 64-65 0.3121562929694548 1101 66-67 0.2370843108534899 1101 68-69 0.12679265861923028 1101 70-71 0.19951507416289616 1101 72-73 0.205719428240144 1101 74-75 0.1567530068832852 1101 76-77 0.1263371410420575 1101 78-79 0.07409643535066124 1101 80-81 0.2376916721669744 1101 82-83 0.2706297934460977 1101 84-85 0.3095547253806288 1101 86-87 0.31482337825873685 1101 88-89 0.22782305227355693 1101 90-91 0.4753920358225727 1101 92-93 0.5959681680963378 1101 94-95 0.4303520428153007 1101 96-97 0.2978917414545492 1101 98-99 0.26469601629823103 1101 100-101 0.7125818587779094 1102 1 0.04424844508967851 1102 2 0.14293011939172118 1102 3 0.12281665590084856 1102 4 0.13896743800613365 1102 5 0.1262144187363532 1102 6 0.12318595719049341 1102 7 0.12021545310426518 1102 8 0.14003798168415926 1102 9 0.15676209655592288 1102 10-11 0.10111979388189951 1102 12-13 0.23715524792354614 1102 14-15 0.10396910802687387 1102 16-17 0.19985277230531295 1102 18-19 0.28055393714666366 1102 20-21 0.24192256690368907 1102 22-23 0.23933681317249977 1102 24-25 0.2120182554606842 1102 26-27 0.2965225455512055 1102 28-29 0.3132115399672486 1102 30-31 0.3527170122421879 1102 32-33 0.2570915215862186 1102 34-35 0.20829195051457816 1102 36-37 0.0969350926991126 1102 38-39 0.23106572333598763 1102 40-41 0.19110890514174628 1102 42-43 0.28395312052133903 1102 44-45 0.3163916298904397 1102 46-47 0.4060198688076042 1102 48-49 0.4335641855210852 1102 50-51 0.3383715316553335 1102 52-53 0.19851778584637003 1102 54-55 0.3150472577586143 1102 56-57 0.20702843428522755 1102 58-59 0.16900161827852855 1102 60-61 0.24159906746941573 1102 62-63 0.4864975358581205 1102 64-65 0.43516653591250787 1102 66-67 0.3896777308141566 1102 68-69 0.43274629821770105 1102 70-71 0.4714811280719289 1102 72-73 0.245278390531098 1102 74-75 0.2691886332261788 1102 76-77 0.2557256339311138 1102 78-79 0.27014335280459534 1102 80-81 0.3739466884806504 1102 82-83 0.3721437822823148 1102 84-85 0.42885971725221594 1102 86-87 0.39424057639886456 1102 88-89 0.4214112654995752 1102 90-91 0.46606623974922456 1102 92-93 0.3603759304509673 1102 94-95 0.49574061528327107 1102 96-97 0.27504122476003445 1102 98-99 0.6491406123215562 1102 100-101 0.6757624005966285 1103 1 0.05884225659197284 1103 2 0.14254743843829942 1103 3 0.16358311118184332 1103 4 0.061862692762311156 1103 5 0.08844927550433823 1103 6 0.14161204509778003 1103 7 0.11642964510076581 1103 8 0.15462632631569306 1103 9 0.13332835502956897 1103 10-11 0.2050723414436746 1103 12-13 0.1696913292705844 1103 14-15 0.22789760135729864 1103 16-17 0.23291367324561207 1103 18-19 0.17605333579087556 1103 20-21 0.12325046981205645 1103 22-23 0.11309173335618539 1103 24-25 0.1604697643170141 1103 26-27 0.11404659978256149 1103 28-29 0.2290886993812009 1103 30-31 0.20198035140685278 1103 32-33 0.3265084798193243 1103 34-35 0.08787592164949842 1103 36-37 0.13816759871266981 1103 38-39 0.21188930741646317 1103 40-41 0.2985247153319932 1103 42-43 0.18481688610192748 1103 44-45 0.39360434583183945 1103 46-47 0.3472783424572867 1103 48-49 0.3800599213618838 1103 50-51 0.35284507200083226 1103 52-53 0.32450045586605114 1103 54-55 0.39406404105185544 1103 56-57 0.36250897222531364 1103 58-59 0.4181050514733684 1103 60-61 0.34454981096383364 1103 62-63 0.4035268382854085 1103 64-65 0.23434103842726728 1103 66-67 0.16403947365255078 1103 68-69 0.0579909406843484 1103 70-71 -0.017181948682939208 1103 72-73 0.013842516925151926 1103 74-75 0.12209974190756867 1103 76-77 0.234900956476487 1103 78-79 0.15708573198675424 1103 80-81 0.16319198697178905 1103 82-83 0.3102738391377713 1103 84-85 0.2859297478666889 1103 86-87 0.18485395929849346 1103 88-89 0.3383667651715605 1103 90-91 0.451162183331121 1103 92-93 0.43826107149623184 1103 94-95 0.3258166047868798 1103 96-97 0.34329783967365657 1103 98-99 0.24306555218597836 1103 100-101 0.49721713490670894 1104 1 -0.007193955580753197 1104 2 0.032414830481826584 1104 3 0.03202921794824931 1104 4 0.022173811429425427 1104 5 0.09128844585455909 1104 6 0.08139018178597723 1104 7 0.017768475430585795 1104 8 0.06719433039594236 1104 9 -0.008889428037413438 1104 10-11 0.05182514661198212 1104 12-13 0.16153934015122928 1104 14-15 0.27016213722944826 1104 16-17 0.2515170394475845 1104 18-19 0.2551824585382505 1104 20-21 0.1491302726002246 1104 22-23 0.12228290982677237 1104 24-25 0.17482500063119488 1104 26-27 0.3018441176336353 1104 28-29 0.24033699927920082 1104 30-31 0.09209213311375208 1104 32-33 0.03560190042456668 1104 34-35 0.07303828139108504 1104 36-37 0.09751178436381025 1104 38-39 0.2548317589635545 1104 40-41 0.3073099924486158 1104 42-43 0.18399233662895398 1104 44-45 0.2101229619488052 1104 46-47 0.23325250090680072 1104 48-49 0.270637105992968 1104 50-51 0.3369831202837261 1104 52-53 0.22595616820878917 1104 54-55 0.1881710624731383 1104 56-57 0.16756252543850536 1104 58-59 0.204861256845696 1104 60-61 0.017057036397353897 1104 62-63 0.21837297905386066 1104 64-65 0.10935803944733635 1104 66-67 0.011818715407059699 1104 68-69 0.23207076047354036 1104 70-71 0.1972667932415817 1104 72-73 0.1453815342661926 1104 74-75 0.1899933155219884 1104 76-77 0.11451460829560034 1104 78-79 0.10657995163993661 1104 80-81 0.28837687306495496 1104 82-83 0.3322072801442246 1104 84-85 0.33745771454469065 1104 86-87 0.17509962403837775 1104 88-89 0.3003524843147076 1104 90-91 0.39626801468100226 1104 92-93 0.5382674468787556 1104 94-95 0.4227266693907552 1104 96-97 0.261641940319695 1104 98-99 0.21295462053008052 1104 100-101 0.24784617264897335 1105 1 0.06847736423733863 1105 2 0.12562084070987112 1105 3 0.08391282808301526 1105 4 0.04246957528476969 1105 5 -0.10613455226307877 1105 6 0.08344328768126275 1105 7 0.12029258797243614 1105 8 0.08101427342537448 1105 9 0.10669460354626636 1105 10-11 0.17281263640892064 1105 12-13 0.10983584529024171 1105 14-15 0.22651177325083438 1105 16-17 0.21817176783792291 1105 18-19 0.23148116254117923 1105 20-21 0.1424295679642853 1105 22-23 0.05401922106596402 1105 24-25 0.15819713336261287 1105 26-27 0.19898684389173837 1105 28-29 -0.01226894598242012 1105 30-31 0.18179578622630999 1105 32-33 0.23365269917854192 1105 34-35 0.09018775416348035 1105 36-37 0.2659445524336377 1105 38-39 0.2877210161806616 1105 40-41 0.3750270886156031 1105 42-43 0.40912185304547677 1105 44-45 0.3084617369150706 1105 46-47 0.32606288220978996 1105 48-49 0.33808246755615556 1105 50-51 0.26288702826460764 1105 52-53 0.2287755448641846 1105 54-55 0.25757374608660655 1105 56-57 0.28990556486812835 1105 58-59 0.3760894556096659 1105 60-61 0.5184511077938012 1105 62-63 0.4750601490160449 1105 64-65 0.3919016419193895 1105 66-67 0.32335567131317333 1105 68-69 0.33254489050634817 1105 70-71 0.24561577126959833 1105 72-73 0.3020089434381248 1105 74-75 0.1968179144791904 1105 76-77 0.18419214989911126 1105 78-79 0.28601224495505306 1105 80-81 0.28660591306095995 1105 82-83 0.17518632430473957 1105 84-85 0.3189152115045957 1105 86-87 0.42296635122932713 1105 88-89 0.3158184518981244 1105 90-91 0.4164221850263239 1105 92-93 0.4939601306588024 1105 94-95 0.4901855656276979 1105 96-97 0.47651321516102385 1105 98-99 0.3870719515757095 1105 100-101 0.6347764669968932 1106 1 -0.09952453023417718 1106 2 -0.07704948110946219 1106 3 -0.05140541904918905 1106 4 -0.12461117254983378 1106 5 -0.15822449149549556 1106 6 -0.04183666864955882 1106 7 -0.041461965582506366 1106 8 -0.008455354824725703 1106 9 -0.033542446215719224 1106 10-11 0.0034952482108394634 1106 12-13 0.023003225059532895 1106 14-15 0.05106243832271673 1106 16-17 0.08878578532164028 1106 18-19 0.0499831653828835 1106 20-21 0.04264871657594682 1106 22-23 -0.05686054421222053 1106 24-25 -0.018443270256149447 1106 26-27 0.028197427244471385 1106 28-29 0.035238338902750854 1106 30-31 0.061181768742592624 1106 32-33 0.08478617111869369 1106 34-35 0.10259614121365246 1106 36-37 0.11923940765909435 1106 38-39 0.11935387402304798 1106 40-41 0.017189716151257528 1106 42-43 0.04871169193511804 1106 44-45 0.05059988081463729 1106 46-47 0.0021734759981768548 1106 48-49 -0.03020961820881496 1106 50-51 -0.07282877692592393 1106 52-53 -0.20379812679821896 1106 54-55 -0.19393059273480873 1106 56-57 -0.0023136559903136344 1106 58-59 0.060461269726687306 1106 60-61 -0.0928631416824075 1106 62-63 -0.1321400468530456 1106 64-65 -0.03742694453192996 1106 66-67 0.010685590316484195 1106 68-69 0.019644021136585366 1106 70-71 0.002565511582503177 1106 72-73 -0.10623060285200125 1106 74-75 0.003463415510715606 1106 76-77 -0.0893422435890514 1106 78-79 -0.09977359948957343 1106 80-81 -0.06047772464544465 1106 82-83 -0.12551286412291773 1106 84-85 0.047011388043650015 1106 86-87 -0.11528531646752427 1106 88-89 -0.1480681848530203 1106 90-91 0.2094030075159452 1106 92-93 0.2715674372180068 1106 94-95 0.21049012600595063 1106 96-97 0.3724617754429467 1106 98-99 0.12714203096690468 1106 100-101 0.09483101332715904 1107 1 0.04887432204974118 1107 2 0.009486845780187991 1107 3 0.0036870474932158004 1107 4 0.017059508330639517 1107 5 0.06107200880818908 1107 6 0.025396874819136883 1107 7 0.006041542491303176 1107 8 0.0070754638654548785 1107 9 0.09726577210166454 1107 10-11 0.06737884299166552 1107 12-13 0.05275745045589275 1107 14-15 0.15017756597471532 1107 16-17 0.1901062871332826 1107 18-19 0.19643425131499015 1107 20-21 0.12154215490715359 1107 22-23 0.05903103012752098 1107 24-25 0.14442874264518224 1107 26-27 0.23424230964545956 1107 28-29 0.1958484295890699 1107 30-31 0.05196653008885477 1107 32-33 0.05452981993431649 1107 34-35 0.05698804018112469 1107 36-37 0.15928380835441658 1107 38-39 0.17887017871235145 1107 40-41 0.060217550360739835 1107 42-43 -0.03347148187684468 1107 44-45 -0.0343672375383548 1107 46-47 0.132251805526721 1107 48-49 0.20970388421017816 1107 50-51 0.16680460318172408 1107 52-53 -0.014893064700068237 1107 54-55 0.16281956957905663 1107 56-57 -0.029402792480574647 1107 58-59 -0.06241241867258651 1107 60-61 0.10061184103901866 1107 62-63 0.12021193337608338 1107 64-65 0.06570990176208369 1107 66-67 0.11777399377639597 1107 68-69 0.2336239570487848 1107 70-71 0.21402382309945267 1107 72-73 0.18005492294018666 1107 74-75 0.1831291536722759 1107 76-77 -0.07147619964911911 1107 78-79 0.09402182221231925 1107 80-81 0.365019165211514 1107 82-83 0.313604776775982 1107 84-85 0.4750136709095898 1107 86-87 0.5369216284714255 1107 88-89 0.4412913560605496 1107 90-91 0.34972753052262817 1107 92-93 0.5660723275776114 1107 94-95 0.5980689267683026 1107 96-97 0.15599686665286683 1107 98-99 0.22753315501658378 1107 100-101 0.35081620611414266 1108 1 -0.2834671519995098 1108 2 -0.15106051047288815 1108 3 -0.11894336535617356 1108 4 -0.07528379091544934 1108 5 -0.14898279779699664 1108 6 -0.10571005636587216 1108 7 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-0.6476937706035315 2316 78-79 -0.8648987524977301 2316 80-81 -0.7491942963703764 2316 82-83 -0.9368471789391322 2316 84-85 -0.7947387883165504 2316 86-87 -0.9023582529137215 2316 88-89 -0.7760248323082735 2316 90-91 -0.8075571978147309 2316 92-93 -0.840500410574073 2316 94-95 -0.7434857858390416 2316 96-97 -0.5164891532015723 2316 98-99 -0.6746378129438533 2316 100-101 -0.6774067536877659 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 20.0 5 31.0 6 61.0 7 128.0 8 267.0 9 529.0 10 874.0 11 1379.0 12 2135.0 13 3231.0 14 3952.0 15 5300.0 16 7140.0 17 9776.0 18 9251.0 19 5542.0 20 4949.0 21 5016.0 22 5434.0 23 6037.0 24 7100.0 25 8844.0 26 10794.0 27 13526.0 28 17072.0 29 20919.0 30 26600.0 31 32967.0 32 41783.0 33 53763.0 34 74708.0 35 113137.0 36 187576.0 37 349202.0 38 424994.0 39 85729.0 40 112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.89700776901821 18.281544219334776 13.141438844049267 23.680009167597753 2 15.825888594577952 19.550769502574102 40.11162886164023 24.511713041207717 3 20.128346996664693 24.205591575491205 28.37544029176148 27.290621136082628 4 12.715794914954854 15.488139397150963 36.54480066082728 35.2512650270669 5 13.68183919869497 37.804502665128894 33.75277942291907 14.760878713257068 6 33.48477291854992 35.25970719948464 17.034550564716394 14.220969317249047 7 30.142841557308063 31.83880905624305 19.681041738100433 18.337307648348453 8 27.251332564446244 35.15677763577611 19.01190216431325 18.5799876354644 9 27.299258127863844 16.005904325509388 19.463363569298547 37.23147397732822 10-11 25.803984929960293 26.339807120895735 26.601483950173925 21.254723998970046 12-13 26.401733892898022 23.456129100340124 27.49535274492455 22.64678426183731 14-15 22.99514640025768 24.850995656826992 25.7858363725154 26.368021570399925 16-17 22.68334205157628 28.388190076528964 26.218370393171654 22.710097478723103 18-19 22.526053842876884 26.734769022162652 28.672961929698783 22.06621520526168 20-21 23.296312230601263 26.01232232874155 28.586568824021235 22.104796616635955 22-23 22.702632497503878 26.082879431127274 28.531142697112617 22.683345374256238 24-25 22.468794018114764 26.274849027404635 28.808019487625085 22.448337466855516 26-27 22.532745306489836 26.592520386871616 28.53389217963696 22.34084212700159 28-29 22.16037959775704 26.604638597309265 28.73287850267609 22.502103302257602 30-31 22.549631769787688 26.37486845442021 28.700396235905018 22.375103539887085 32-33 22.191083017638945 26.468810953207093 28.716548490300575 22.62355753885338 34-35 22.649400999767835 26.46309804641014 28.521405389784444 22.36609556403758 36-37 22.772132881222092 26.353500728787477 28.356895134803768 22.517471255186663 38-39 22.473734265479955 26.48276350237501 28.493950690521668 22.549551541623362 40-41 22.66424958657345 26.33769829943934 28.603434890310865 22.394617223676345 42-43 22.618459655121466 26.389750733447308 28.337156838391564 22.654632773039662 44-45 22.568964811823818 26.633207928006563 28.209899032800863 22.587928227368756 46-47 22.52129461932732 26.790772834986143 27.90323382042471 22.78469872526182 48-49 22.806903595500874 26.76752406824356 27.733371437664506 22.69220089859106 50-51 22.820450207464837 26.905354599588645 27.49449758699138 22.77969760595514 52-53 22.938591833957947 26.9238050611315 27.4414849899773 22.69611811493326 54-55 22.7534288386108 26.830077668389745 27.589325142218872 22.827168350780582 56-57 22.728193725029744 27.117601140903684 27.4418897645977 22.71231536946888 58-59 22.843899516676057 26.853880959764133 27.517676148604657 22.78454337495515 60-61 22.888401758992792 26.883768865775654 27.655041031367496 22.572788343864065 62-63 22.75525882401837 26.83510099435803 27.628080743534593 22.781559438089005 64-65 22.61966919285394 26.81684168144064 27.666458856916492 22.897030268788924 66-67 22.69350108579325 27.033979094583504 27.42728691956818 22.845232900055066 68-69 22.6825262616502 26.929750735118397 27.55398444562202 22.833738557609383 70-71 22.615992352680653 26.853416421735115 27.62259673768058 22.90799448790365 72-73 22.66988860040705 26.969559609887117 27.369985102561138 22.990566687144693 74-75 22.61356020697609 27.09982803944183 27.292017341520943 22.994594412061137 76-77 22.6897793109865 27.05128025783759 27.365151390444563 22.893789040731352 78-79 22.73024971827914 26.98666429177148 27.36126283751654 22.921823152432843 80-81 22.496337338881876 27.236398798854967 27.363000888390143 22.904262973873017 82-83 22.72899067984165 27.266366125328595 27.1723333125526 22.83230988227715 84-85 22.761328029872555 26.987580160727333 27.17853721893011 23.072554590470006 86-87 22.598961707899154 26.936979785195962 27.323486604250373 23.140571902654507 88-89 22.81351957752137 26.952118435104655 27.230896852638697 23.003465134735283 90-91 22.70793388273634 27.151382426117042 27.277393585058185 22.86329010608843 92-93 22.693855034593884 27.004006156102662 27.291099898592343 23.01103891071111 94-95 22.616656373409498 27.10834805886055 27.253849905477377 23.02114566225258 96-97 22.65155337870177 27.058253568397173 27.126832255621864 23.163360797279193 98-99 22.600157160205995 26.939045219277453 27.052142119584627 23.40865550093192 100-101 22.598425196850393 27.111811023622046 26.984852666612554 23.304911112915008 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 420.0 1 278.0 2 353.0 3 563.5 4 412.5 5 398.0 6 859.5 7 1588.5 8 2223.5 9 2279.5 10 1750.0 11 1291.0 12 1248.0 13 1309.0 14 1344.0 15 1414.5 16 1448.5 17 1534.5 18 1642.0 19 1762.5 20 1990.5 21 2301.5 22 2651.5 23 3279.0 24 4038.0 25 4866.0 26 6362.0 27 8243.0 28 10027.0 29 12271.5 30 14791.5 31 17238.0 32 20627.5 33 24250.5 34 27904.5 35 31011.0 36 34391.5 37 37744.0 38 40190.5 39 42811.0 40 44908.5 41 48165.5 42 50788.0 43 52834.0 44 55577.5 45 57546.0 46 58449.5 47 58746.0 48 61050.0 49 60767.0 50 58040.5 51 55877.5 52 54034.0 53 53715.0 54 52290.0 55 48644.5 56 44548.0 57 40648.0 58 37513.5 59 33940.0 60 29381.0 61 24726.0 62 20475.0 63 16233.0 64 12403.0 65 9728.5 66 7100.5 67 5459.0 68 3880.5 69 2438.0 70 1864.5 71 1491.5 72 1290.0 73 1295.0 74 1058.0 75 803.5 76 569.0 77 300.0 78 173.5 79 101.0 80 56.5 81 36.0 82 20.0 83 9.5 84 3.5 85 3.5 86 2.5 87 1.0 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26261650197936476 2 0.020910611680849518 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.468075600303399E-4 12-13 3.246989391436261E-5 14-15 0.0 16-17 8.766871356877902E-4 18-19 0.0 20-21 3.246989391436261E-5 22-23 3.246989391436261E-5 24-25 0.002532651725320283 26-27 0.003182049603607535 28-29 0.0030846399218644473 30-31 0.0014611452261463172 32-33 0.0018507839531186684 34-35 0.001980663528776119 36-37 9.740968174308782E-5 38-39 9.740968174308782E-5 40-41 8.766871356877902E-4 42-43 0.004578255041925127 44-45 0.005260122814126742 46-47 0.015488139397150963 48-49 0.01659211579023929 50-51 0.007078436873331048 52-53 0.008669461675134814 54-55 5.195183026298017E-4 56-57 0.0036366281184086117 58-59 0.001980663528776119 60-61 0.0019157237409473935 62-63 0.0 64-65 0.0011689161809170538 66-67 0.0 68-69 0.0 70-71 1.2987957565745043E-4 72-73 0.0016884344835468553 74-75 0.0 76-77 0.19166978377648244 78-79 0.10224769593632783 80-81 0.0015585549078894051 82-83 0.0 84-85 8.442172417734276E-4 86-87 0.12218421079974648 88-89 0.579457726795715 90-91 0.30563911141589517 92-93 0.29229398501709214 94-95 0.0029222904522926345 96-97 0.0033119291792649856 98-99 0.003766507694066062 100-101 8.442172417734276E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1539888.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.956751111115636 #Duplication Level Percentage of deduplicated Percentage of total 1 70.71173813529697 36.73952178930009 2 15.55888685204743 16.167784234756756 3 5.784293451299072 9.01599285608406 4 2.747598904541852 5.7102524974582 5 1.5133815287479555 3.931519371265861 6 0.898987246402114 2.8025073968029095 7 0.5990422604917458 2.178700274338682 8 0.4119457418239326 1.7122689903383972 9 0.3056175595128009 1.4291005927313851 >10 1.3333578307554608 12.069367064835841 >50 0.09252524495747833 3.3112255894740636 >100 0.04050034455292765 3.500028842174621 >500 0.001499929108403461 0.4909031503592335 >1k 4.999763694678203E-4 0.5793553385837705 >5k 1.2499409236695508E-4 0.36147201149616265 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5566 0.3614548590546845 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4035 0.2620320438889062 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2789 0.18111706825431462 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 6.49397878287252E-5 0.0 0.0 0.0 14-15 0.0 6.49397878287252E-5 0.0 0.0 0.0 16-17 0.0 6.49397878287252E-5 0.0 9.74096817430878E-5 0.0 18-19 0.0 6.49397878287252E-5 0.0 2.272892574005382E-4 0.0 20-21 0.0 6.49397878287252E-5 0.0 4.545785148010764E-4 0.0 22-23 0.0 6.49397878287252E-5 0.0 0.0011689161809170538 0.0 24-25 0.0 9.74096817430878E-5 0.0 0.0017533742713755805 0.0 26-27 0.0 1.298795756574504E-4 0.0 0.0018183140592043059 0.0 28-29 0.0 1.298795756574504E-4 0.0 0.0024352420435771955 0.0 30-31 0.0 1.298795756574504E-4 0.0 0.004675664723668215 0.0 32-33 0.0 1.298795756574504E-4 0.0 0.008377232629905551 0.0 34-35 0.0 1.298795756574504E-4 0.0 0.012663258626601415 0.0 36-37 0.0 1.298795756574504E-4 0.0 0.021300250407821866 0.0 38-39 0.0 1.298795756574504E-4 0.0 0.034872666064025434 0.0 40-41 0.0 1.298795756574504E-4 0.0 0.05217911952038071 0.0 42-43 0.0 1.6234946957181302E-4 0.0 0.06847900626539073 0.0 44-45 0.0 1.948193634861756E-4 0.0 0.08805835229575137 0.0 46-47 0.0 1.948193634861756E-4 0.0 0.1123458329436946 0.0 48-49 0.0 1.948193634861756E-4 0.0 0.1395231341500161 0.0 50-51 0.0 1.948193634861756E-4 0.0 0.17092152156520474 0.0 52-53 0.0 1.948193634861756E-4 0.0 0.19949502820984383 0.0 54-55 0.0 1.948193634861756E-4 0.0 0.23209480169986388 0.0 56-57 0.0 1.948193634861756E-4 0.0 0.2697923485344389 0.0 58-59 0.0 1.948193634861756E-4 0.0 0.31391893436405766 0.0 60-61 0.0 1.948193634861756E-4 0.0 0.3622990762964579 0.0 62-63 0.0 1.948193634861756E-4 0.0 0.4143483162411812 0.0 64-65 0.0 1.948193634861756E-4 0.0 0.4674690626850784 0.0 66-67 0.0 2.272892574005382E-4 0.0 0.5272786072753344 0.0 68-69 0.0 2.9222904522926345E-4 0.0 0.600595627733965 0.0 70-71 0.0 3.2469893914362604E-4 0.0 0.6826470496555593 0.0 72-73 0.0 3.2469893914362604E-4 0.0 0.7653803393493552 0.0 74-75 0.0 3.2469893914362604E-4 0.0 0.8465875440291761 0.0 76-77 0.0 3.2469893914362604E-4 0.0 0.9413022245773719 0.0 78-79 0.0 3.2469893914362604E-4 0.0 1.0564079985037873 0.0 80-81 0.0 3.2469893914362604E-4 0.0 1.1887552860987292 0.0 82-83 0.0 3.2469893914362604E-4 0.0 1.323245586692019 0.0 84-85 0.0 3.2469893914362604E-4 0.0 1.457021549619193 0.0 86-87 0.0 3.2469893914362604E-4 0.0 1.6018697463711646 0.0 88-89 0.0 3.2469893914362604E-4 0.0 1.7626606610350883 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5925 0.0 40.140774 1 GGTATCA 3415 0.0 38.164886 1 ATCAACG 7250 0.0 32.254063 3 TCAACGC 7290 0.0 32.01202 4 CAACGCA 7430 0.0 31.34601 5 AACGCAG 7690 0.0 30.59461 6 TATCAAC 7695 0.0 30.457382 2 TATAACG 130 3.0699812E-7 29.19583 2 GTACATG 7705 0.0 28.27463 1 TACATGG 8040 0.0 26.613052 2 ACGCAGA 8935 0.0 26.33157 7 CGCAGAG 9040 0.0 25.868313 8 ACATGGG 7840 0.0 25.833742 3 GCAGAGT 9660 0.0 23.913406 9 GTACCTG 1365 0.0 21.953917 1 CATGGGA 4565 0.0 20.988726 4 GAGTACT 4960 0.0 20.943634 12-13 AGTACAT 5570 0.0 20.78303 2 AGTACTT 5290 0.0 20.757973 12-13 GTATTCG 115 0.0036936349 20.681225 1 >>END_MODULE