FastQCFastQC Report
Thu 26 May 2016
SRR936346_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936346_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729798
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34220.19782656703268242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22200.12833868463254092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.048.0109561
GTATCAA38250.044.8125341
ATCAACG47500.035.6778533
TCAACGC48100.035.134394
CAACGCA49750.033.969135
TATCAAC50400.033.9057582
AACGCAG50500.033.742936
GTACATG61850.030.852311
GTACCTG16900.029.6989781
TACATGG62500.029.3865412
ACGCAGA60450.027.797377
CGCAGAG61200.027.3020298
ACATGGG64850.027.3005243
TACCTGG19000.025.4122642
CATGGGG42900.024.4965524
GCAGAGT68750.023.6841269
AGTACAT42300.023.276612
TAGCCCT11550.022.5418764
GAGTACA47750.022.41081
ATGGGGA20750.021.4446545
Produced by FastQC (version 0.11.5)