##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936346_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1729798 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.511274148773442 33.0 31.0 34.0 30.0 34.0 2 31.60383987032012 33.0 31.0 34.0 30.0 34.0 3 31.67568640962702 34.0 31.0 34.0 30.0 34.0 4 35.15712932955177 37.0 35.0 37.0 33.0 37.0 5 35.12973191089364 37.0 35.0 37.0 33.0 37.0 6 35.21771386023108 37.0 35.0 37.0 33.0 37.0 7 35.155332009864736 37.0 35.0 37.0 32.0 37.0 8 35.15369309017585 37.0 35.0 37.0 32.0 37.0 9 36.79972285781346 39.0 37.0 39.0 33.0 39.0 10-11 36.70925391288462 39.0 37.0 39.0 33.0 39.0 12-13 36.601571686405 39.0 37.0 39.0 32.0 39.0 14-15 37.82463761664657 40.0 38.0 41.0 32.5 41.0 16-17 37.76920050780495 40.0 38.0 41.0 32.0 41.0 18-19 37.7045539421366 40.0 38.0 41.0 32.0 41.0 20-21 37.619068816127665 40.0 38.0 41.0 32.0 41.0 22-23 37.45545491438885 40.0 37.5 41.0 32.0 41.0 24-25 37.604784489287184 40.0 38.0 41.0 32.0 41.0 26-27 37.553659733679886 40.0 38.0 41.0 32.0 41.0 28-29 37.4103681470322 40.0 38.0 41.0 32.0 41.0 30-31 37.26218986263136 40.0 37.0 41.0 31.0 41.0 32-33 37.07731567500945 40.0 37.0 41.0 31.0 41.0 34-35 36.81679016856303 40.0 37.0 41.0 30.0 41.0 36-37 36.623838159137655 40.0 36.0 41.0 30.0 41.0 38-39 36.460699746444384 40.0 36.0 41.0 30.0 41.0 40-41 36.27131896325467 40.0 36.0 41.0 29.5 41.0 42-43 36.08946738289673 40.0 35.5 41.0 29.0 41.0 44-45 35.904537986516345 39.0 35.0 41.0 28.0 41.0 46-47 35.635708331261796 39.0 35.0 41.0 27.0 41.0 48-49 35.475741676195724 39.0 35.0 41.0 26.5 41.0 50-51 35.17615698480401 38.5 34.5 40.5 26.5 41.0 52-53 35.20101508962318 39.0 35.0 40.5 26.0 41.0 54-55 35.27851431207574 39.0 35.0 41.0 26.0 41.0 56-57 35.115983484776834 39.0 35.0 41.0 26.0 41.0 58-59 34.885129072874406 38.5 35.0 41.0 26.0 41.0 60-61 34.590120927414645 38.0 34.0 40.0 25.5 41.0 62-63 34.28603368717041 37.5 34.0 40.0 25.0 41.0 64-65 33.955657539204 37.0 34.0 40.0 23.5 41.0 66-67 33.600495549191294 36.5 34.0 39.5 23.0 41.0 68-69 33.22064108063485 36.0 33.0 39.0 22.0 41.0 70-71 32.81854124007543 35.5 33.0 39.0 21.5 40.5 72-73 32.40864251201585 35.0 33.0 38.0 20.0 40.0 74-75 31.991014557769173 35.0 32.0 37.0 19.0 39.5 76-77 31.584762787331236 35.0 32.0 37.0 17.5 39.0 78-79 31.193802108685524 35.0 31.5 36.0 14.5 39.0 80-81 30.793920157151298 35.0 31.0 36.0 9.5 37.5 82-83 30.447417848789282 35.0 31.0 35.0 7.0 37.0 84-85 30.129992345927096 34.5 31.0 35.0 4.0 36.5 86-87 29.801670773119174 34.0 30.5 35.0 2.0 36.0 88-89 29.48552952425659 34.0 30.0 35.0 2.0 36.0 90-91 29.13957785822391 34.0 30.0 35.0 2.0 35.5 92-93 28.823199009364096 34.0 29.5 35.0 2.0 35.0 94-95 28.451029253126663 34.0 29.0 35.0 2.0 35.0 96-97 28.028893836158904 34.0 29.0 35.0 2.0 35.0 98-99 27.527416785081265 34.0 29.0 35.0 2.0 35.0 100-101 24.35930553740957 30.0 14.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48509828557508783 1101 2 0.4489345583242468 1101 3 0.37029600160557763 1101 4 0.2621232912384812 1101 5 0.45023806595324345 1101 6 0.3200620930955793 1101 7 0.4411584332624372 1101 8 0.4197337160518728 1101 9 0.3960784946483855 1101 10-11 0.5292515583346358 1101 12-13 0.48849581222672356 1101 14-15 0.623691062790158 1101 16-17 0.6967173708645475 1101 18-19 0.7614144089948383 1101 20-21 0.7045967708190162 1101 22-23 0.9003287137758278 1101 24-25 0.6234329524759445 1101 26-27 0.5534319241474108 1101 28-29 0.5735383892715689 1101 30-31 0.6732274346812801 1101 32-33 0.7681842262449976 1101 34-35 0.42027995533042173 1101 36-37 0.38889620335094577 1101 38-39 0.45840003342352276 1101 40-41 0.2738526785775264 1101 42-43 0.25996405693852864 1101 44-45 0.3079873837591762 1101 46-47 0.13908037988328914 1101 48-49 0.25538650102112825 1101 50-51 0.4138621252736385 1101 52-53 0.4983504576321707 1101 54-55 0.4751369384156021 1101 56-57 0.3717683243962071 1101 58-59 0.4401269884422234 1101 60-61 0.2876291247365046 1101 62-63 0.29154648327924093 1101 64-65 0.2676675598815592 1101 66-67 0.3262421255921808 1101 68-69 0.29060792191965135 1101 70-71 0.46546552761223836 1101 72-73 0.4628347256235017 1101 74-75 0.3401304527192508 1101 76-77 0.3782203640672641 1101 78-79 0.519765865186887 1101 80-81 0.5347832541074418 1101 82-83 0.665650068415431 1101 84-85 0.5480255443856699 1101 86-87 0.4898327436269021 1101 88-89 0.4048023405154275 1101 90-91 0.5402554302475941 1101 92-93 0.6703956637587538 1101 94-95 0.8913337586171828 1101 96-97 0.9890023653996529 1101 98-99 0.907676643055833 1101 100-101 1.0111152342989236 1102 1 0.33296078365015447 1102 2 0.3729808710101423 1102 3 0.29564694573309325 1102 4 0.36814276381440436 1102 5 0.42426569161052896 1102 6 0.42078690756626 1102 7 0.3339961104647813 1102 8 0.4655917707667996 1102 9 0.44940530482450924 1102 10-11 0.5005431780674456 1102 12-13 0.4784095750502715 1102 14-15 0.5701217970692696 1102 16-17 0.4975032642342896 1102 18-19 0.5340533980958071 1102 20-21 0.48287647411052603 1102 22-23 0.5113523736303804 1102 24-25 0.6270196075151304 1102 26-27 0.5973436296675274 1102 28-29 0.5826760002041738 1102 30-31 0.48707954069209336 1102 32-33 0.520363947012342 1102 34-35 0.4760373769357429 1102 36-37 0.4700532764777918 1102 38-39 0.5351282375135611 1102 40-41 0.5151225486853761 1102 42-43 0.5677798633962823 1102 44-45 0.4656935769519066 1102 46-47 0.6339957345648202 1102 48-49 0.6015650036710554 1102 50-51 0.5189420555779449 1102 52-53 0.43493070544293033 1102 54-55 0.37699110692069127 1102 56-57 0.43257405543104 1102 58-59 0.42826678125844353 1102 60-61 0.47681712143512556 1102 62-63 0.7225554444015643 1102 64-65 0.6044830825733243 1102 66-67 0.6042638008150831 1102 68-69 0.634587021862373 1102 70-71 0.8026502488900107 1102 72-73 0.7052134891997142 1102 74-75 0.6493843384965245 1102 76-77 0.42861261863996347 1102 78-79 0.5517914093168237 1102 80-81 0.580299794723178 1102 82-83 0.4564291012232786 1102 84-85 0.5412132140612975 1102 86-87 0.491977642500661 1102 88-89 0.4869186378537371 1102 90-91 0.4216700123589163 1102 92-93 0.5130900304847685 1102 94-95 0.5839762601874128 1102 96-97 0.5074975491640892 1102 98-99 0.573420637685043 1102 100-101 0.9317580853857983 1103 1 0.46843107453731037 1103 2 0.5167169279201431 1103 3 0.5489014431392611 1103 4 0.4357713742212681 1103 5 0.47606962148510945 1103 6 0.36061659524527556 1103 7 0.48989999049618405 1103 8 0.38280601483941723 1103 9 0.425404482982799 1103 10-11 0.4997545228651319 1103 12-13 0.5150369125325582 1103 14-15 0.5735482513253558 1103 16-17 0.6741941591666389 1103 18-19 0.5896955485985274 1103 20-21 0.6340484229280463 1103 22-23 0.7009631783123424 1103 24-25 0.8311436971584456 1103 26-27 0.6920860410542602 1103 28-29 0.5611671258778941 1103 30-31 0.5413346219271773 1103 32-33 0.6516762007821768 1103 34-35 0.48493749146090437 1103 36-37 0.4837628983249189 1103 38-39 0.7057584725797383 1103 40-41 0.5723707571423091 1103 42-43 0.5387289848838464 1103 44-45 0.4974682857878463 1103 46-47 0.5604590799594433 1103 48-49 0.6271093152708147 1103 50-51 0.46442761225944196 1103 52-53 0.5447461334918273 1103 54-55 0.5910536049390132 1103 56-57 0.6878417268245656 1103 58-59 0.6711351694805714 1103 60-61 0.7177232780188021 1103 62-63 0.7035564848092051 1103 64-65 0.6001195850280752 1103 66-67 0.6822305373636581 1103 68-69 0.504767315501681 1103 70-71 0.5629806596967271 1103 72-73 0.561793443956141 1103 74-75 0.6509669333771697 1103 76-77 0.5633479446012366 1103 78-79 0.5391004230687209 1103 80-81 0.4313403503287567 1103 82-83 0.619357106790158 1103 84-85 0.6913692752073324 1103 86-87 0.7975471843027684 1103 88-89 0.6108541858475576 1103 90-91 0.7879495171739173 1103 92-93 0.770000751164698 1103 94-95 0.685403322369627 1103 96-97 0.7877673449169755 1103 98-99 0.8693874410293958 1103 100-101 0.8890696652792549 1104 1 0.28672552395472906 1104 2 0.21803640902141552 1104 3 0.28171043171824905 1104 4 0.2543174004198434 1104 5 0.3290479035327394 1104 6 0.3121112742522456 1104 7 0.37319224530764217 1104 8 0.43088559051049913 1104 9 0.4230797652964142 1104 10-11 0.46042928390836835 1104 12-13 0.5056748387822623 1104 14-15 0.5225848069551162 1104 16-17 0.567833384063114 1104 18-19 0.41150507327076014 1104 20-21 0.3763701842784428 1104 22-23 0.5340555716351432 1104 24-25 0.6251455467212921 1104 26-27 0.47029084514141317 1104 28-29 0.5694475429930179 1104 30-31 0.4864657671326853 1104 32-33 0.5675499133555064 1104 34-35 0.5110150100491708 1104 36-37 0.36518109140247645 1104 38-39 0.28103551584847253 1104 40-41 0.39470916949930057 1104 42-43 0.4365634893599406 1104 44-45 0.40671412365381343 1104 46-47 0.4254729022712951 1104 48-49 0.4433699455221216 1104 50-51 0.37362774632392615 1104 52-53 0.25327590395993127 1104 54-55 0.37570367440055463 1104 56-57 0.43600472222700404 1104 58-59 0.34351982090879574 1104 60-61 0.2987641470992344 1104 62-63 0.3662383427057918 1104 64-65 0.5752233655971253 1104 66-67 0.4952682261183057 1104 68-69 0.47310137488039317 1104 70-71 0.4643899425917297 1104 72-73 0.48356912311052014 1104 74-75 0.42293069808577144 1104 76-77 0.4816787511868661 1104 78-79 0.640967402772116 1104 80-81 0.5845487571930583 1104 82-83 0.645839165783471 1104 84-85 0.481513271004232 1104 86-87 0.47935031867771016 1104 88-89 0.5564252816106112 1104 90-91 0.6186484312351261 1104 92-93 0.5415171538884778 1104 94-95 0.3307412052213188 1104 96-97 0.7258716335805353 1104 98-99 0.7430678981662311 1104 100-101 0.7117074169486806 1105 1 0.22129115116516118 1105 2 0.18457787791614422 1105 3 0.13301740683979446 1105 4 0.15525246294404837 1105 5 0.09531987982251167 1105 6 0.08789310686596963 1105 7 0.16087160031774062 1105 8 0.17965895107047913 1105 9 0.2292019203410831 1105 10-11 0.32646073324728064 1105 12-13 0.2752218543597067 1105 14-15 0.1460529079284285 1105 16-17 0.2597748510374629 1105 18-19 0.20546845492285826 1105 20-21 0.3238215267804989 1105 22-23 0.45633480054062403 1105 24-25 0.29219535484384096 1105 26-27 0.29367957062063255 1105 28-29 0.32084661609209064 1105 30-31 0.39195091783410163 1105 32-33 0.36522001856003783 1105 34-35 0.23125200030967363 1105 36-37 0.3288988091009699 1105 38-39 0.34907879789712837 1105 40-41 0.17496947631119042 1105 42-43 0.2445492081722591 1105 44-45 0.24900973975807972 1105 46-47 0.3770254453277033 1105 48-49 0.5828507721794836 1105 50-51 0.38771609636985715 1105 52-53 0.4844890367634491 1105 54-55 0.4937363143718798 1105 56-57 0.6018618975476286 1105 58-59 0.6504661368296638 1105 60-61 0.5495186943868688 1105 62-63 0.40266585335587024 1105 64-65 0.3760316833807522 1105 66-67 0.28572772381225064 1105 68-69 0.4146783707099715 1105 70-71 0.43491979001795045 1105 72-73 0.3627171328020111 1105 74-75 0.4539691567929154 1105 76-77 0.5360945731553137 1105 78-79 0.4633455309827461 1105 80-81 0.4849633181153514 1105 82-83 0.48960826951251946 1105 84-85 0.5892607033030153 1105 86-87 0.6180581944550632 1105 88-89 0.5376296126960405 1105 90-91 0.6157386632484148 1105 92-93 0.5571850122544326 1105 94-95 0.5184918541479853 1105 96-97 0.841729048948018 1105 98-99 0.786249527220221 1105 100-101 0.7911565254146531 1106 1 0.23851887937596317 1106 2 0.2162519600116113 1106 3 0.22982524129917437 1106 4 0.1496547950767848 1106 5 0.2736868376134183 1106 6 0.20566659494242856 1106 7 0.05912367781485983 1106 8 0.026640596903320102 1106 9 0.19529967844901108 1106 10-11 0.2160810946807743 1106 12-13 0.17517908813011474 1106 14-15 0.26455577601226565 1106 16-17 0.18178062644020798 1106 18-19 0.2171450088279272 1106 20-21 0.2682097342869483 1106 22-23 0.11347087562085534 1106 24-25 0.20249436533529774 1106 26-27 0.19787340757536498 1106 28-29 0.21541432405759053 1106 30-31 0.3081712855452068 1106 32-33 0.22436806818456034 1106 34-35 0.06256492355802123 1106 36-37 0.1813980832521196 1106 38-39 0.18424578236788847 1106 40-41 -0.032475771144412136 1106 42-43 0.04737884163820638 1106 44-45 0.171179125415307 1106 46-47 0.18510924359665637 1106 48-49 0.18386225236184828 1106 50-51 0.1804164110351465 1106 52-53 0.3067098203663292 1106 54-55 0.18487193432526539 1106 56-57 0.25931656142076065 1106 58-59 0.10363790013231267 1106 60-61 0.2047118486513213 1106 62-63 0.16967641543752876 1106 64-65 0.17434182878593418 1106 66-67 0.0812602222232357 1106 68-69 0.17661505314914194 1106 70-71 0.10449867997926532 1106 72-73 0.08749427758727535 1106 74-75 0.05728589369035575 1106 76-77 0.09880300948071508 1106 78-79 0.15322955210691092 1106 80-81 0.20535908229686584 1106 82-83 0.07908640380632548 1106 84-85 0.09534017786691962 1106 86-87 0.14042336312664006 1106 88-89 0.05643537554356115 1106 90-91 0.21445430313321978 1106 92-93 0.032140088095829356 1106 94-95 0.18977943153916854 1106 96-97 0.2725974388190906 1106 98-99 0.09140992019871419 1106 100-101 0.21502832089006318 1107 1 0.06599409424805458 1107 2 0.0648095010833849 1107 3 0.13926540690543376 1107 4 0.09835135610858003 1107 5 0.12445246213776073 1107 6 0.11240348191007143 1107 7 0.056414310710835025 1107 8 0.05559327937970693 1107 9 0.12197426012070167 1107 10-11 0.15751817546455982 1107 12-13 0.2013432534522437 1107 14-15 0.3493122881333903 1107 16-17 0.036804117769214884 1107 18-19 0.22680334444443417 1107 20-21 0.3386756033646563 1107 22-23 0.30999334713191473 1107 24-25 0.35007673719847077 1107 26-27 0.23515308123361223 1107 28-29 0.28454073074679087 1107 30-31 0.24463206060684684 1107 32-33 0.2872192965324558 1107 34-35 0.23320126966698496 1107 36-37 0.23886812292862203 1107 38-39 0.238617807898855 1107 40-41 0.3465667161966479 1107 42-43 0.08930097016570926 1107 44-45 0.3449155151582701 1107 46-47 0.45504237195164876 1107 48-49 0.22643990566433558 1107 50-51 0.3244644287322842 1107 52-53 0.38906037390930237 1107 54-55 0.5183980690630108 1107 56-57 0.3926884550349783 1107 58-59 0.4121424715550859 1107 60-61 0.49651981845253346 1107 62-63 0.6022537643167425 1107 64-65 0.39031593251309005 1107 66-67 0.3732934652443376 1107 68-69 0.40731512519598567 1107 70-71 0.39101374385740684 1107 72-73 0.3066079999735436 1107 74-75 0.30611944519370127 1107 76-77 0.3428662483413625 1107 78-79 0.2953637621792602 1107 80-81 0.3878770686384101 1107 82-83 0.4844939758277995 1107 84-85 0.4780458918916999 1107 86-87 0.19673253539976798 1107 88-89 0.315874019033469 1107 90-91 0.4970666606884997 1107 92-93 0.2874761206488259 1107 94-95 0.4336833555551216 1107 96-97 0.7363517895349929 1107 98-99 0.5780690777580517 1107 100-101 0.7143159704379975 1108 1 -0.27235656599497915 1108 2 -0.26411441005199876 1108 3 -0.07987871527366153 1108 4 -0.2967626504060803 1108 5 -0.1416693658674646 1108 6 -0.20540845122691564 1108 7 -0.2513525659592801 1108 8 -0.19627432092457298 1108 9 -0.27188557720790385 1108 10-11 -0.36216428372129883 1108 12-13 -0.38885452688284516 1108 14-15 -0.49129607169418676 1108 16-17 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-1.9751876552665735 2316 62-63 -1.9954513871988624 2316 64-65 -1.7926521468521557 2316 66-67 -1.8462081381465012 2316 68-69 -1.9282687038232353 2316 70-71 -1.7489743923884404 2316 72-73 -1.691295475452275 2316 74-75 -1.526526246315985 2316 76-77 -1.7955787078534335 2316 78-79 -1.838125623626162 2316 80-81 -1.5640922699498638 2316 82-83 -1.780822086178901 2316 84-85 -1.9128923110320173 2316 86-87 -2.0510199670644127 2316 88-89 -1.951054056585324 2316 90-91 -2.0206219291452463 2316 92-93 -2.113066371066619 2316 94-95 -1.9911817648590109 2316 96-97 -2.1922631989357555 2316 98-99 -1.9717496208862428 2316 100-101 -1.9628241512026499 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20369.0 3 3934.0 4 3289.0 5 2764.0 6 2630.0 7 2807.0 8 3349.0 9 3984.0 10 4513.0 11 5305.0 12 6799.0 13 9176.0 14 9128.0 15 8171.0 16 9387.0 17 10927.0 18 10648.0 19 8250.0 20 8367.0 21 8638.0 22 9204.0 23 10062.0 24 11632.0 25 13361.0 26 15465.0 27 18755.0 28 22545.0 29 27042.0 30 32623.0 31 40699.0 32 51463.0 33 67025.0 34 92970.0 35 137816.0 36 226038.0 37 371013.0 38 371629.0 39 67952.0 40 69.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.6386257699086 17.473375289700794 12.462672193755823 24.425326746634784 2 15.997264070926013 18.583387730063478 40.142821048152236 25.276527150858275 3 20.41395554825781 22.78183586319659 27.60831079448152 29.19589779406408 4 13.001545931033206 14.492554208759659 35.56589516575688 36.94000469445026 5 14.573074666042258 36.705142992596706 33.427457238087804 15.294325103273234 6 35.23485160329493 33.90010221496594 16.532923856788045 14.332122324951088 7 31.26563134142492 30.700735041608418 19.28492719538324 18.74870642158342 8 27.54218277351736 35.32055670160313 18.468376795774322 18.668883729105186 9 27.065951564055684 16.04945124200511 19.03047165087636 37.85412554306284 10-11 26.558379561018373 25.61009415503423 26.318489888877227 21.513036395070166 12-13 26.621591659051862 22.369915511100462 27.30806577455192 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23.452579735565127 26.04782246331569 27.253083282755973 23.24651451836321 40-41 23.59724767711035 25.934514698322058 27.227316427092198 23.24092119747539 42-43 23.669632687058805 25.809953584172035 27.053294849998053 23.467118878771103 44-45 23.575227058573386 26.12291894441727 27.031588939456082 23.270265057553264 46-47 23.500364707259607 26.310484251647136 26.781626190599994 23.407524850493264 48-49 23.88481195677151 26.192651528774498 26.640437827745494 23.282098686708498 50-51 23.582489277768126 26.341390284591654 26.68544304128459 23.390677396355635 52-53 23.702315090794368 26.41504884547585 26.500370103105965 23.38226596062382 54-55 23.6514008177791 26.151009414306458 26.608929692317655 23.588660075596785 56-57 23.481158841065987 26.564006729234297 26.66909292431238 23.285741505387335 58-59 23.68921842215938 26.2434853022843 26.634860922966823 23.432435352589497 60-61 23.807988614662978 26.207041772859625 26.83262538029785 23.15234423217955 62-63 23.55214754929666 26.37437288237983 26.73543928293235 23.33804028539116 64-65 23.494479338804567 26.243147283763818 26.729783452278838 23.532589925152784 66-67 23.49685246745236 26.51774028159214 26.496657026256205 23.488750224699295 68-69 23.197243481168176 26.353663201943412 26.78888620035479 23.660207116533623 70-71 23.305872587794322 26.233511790423957 26.82072410169789 23.63989152008383 72-73 23.482963867872495 26.373917460826434 26.599683352662705 23.543435318638366 74-75 23.42839099528338 26.492524198207878 26.437099474307534 23.641985332201212 76-77 23.553298578284398 26.520693137547912 26.315491381961937 23.610516902205752 78-79 23.539536417012663 26.44113431301219 26.440495328880775 23.578833941094377 80-81 23.339281896848192 26.752560670700536 26.497626383738115 23.41053104871316 82-83 23.557486893901967 26.762377707158258 26.370581693201267 23.30955370573851 84-85 23.593362102243002 26.424167045956576 26.259912972934824 23.7225578788656 86-87 23.381566514822723 26.367372499447395 26.68060691491695 23.57045407081294 88-89 23.59253402991396 26.459770522310556 26.42186025257826 23.52583519519722 90-91 23.593489575463693 26.699319133307142 26.381230720593603 23.325960570635562 92-93 23.53549939132279 26.486726769304074 26.48829567476009 23.489478164613047 94-95 23.457944766735146 26.60997784128388 26.486999726102116 23.44507766587886 96-97 23.491466048585067 26.565680558596473 26.347402587974706 23.595450804843757 98-99 23.458329365143886 26.382236525770775 26.309531552332484 23.849902556752852 100-101 23.520437423140976 26.59578576603495 26.130138030463396 23.753638780360678 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 79.0 1 62.5 2 106.5 3 191.5 4 162.0 5 156.0 6 323.5 7 584.0 8 924.5 9 1127.0 10 1104.5 11 1053.5 12 1143.0 13 1339.5 14 1552.0 15 1732.0 16 1867.0 17 1994.5 18 2072.5 19 2139.0 20 2177.0 21 2248.0 22 2483.0 23 2994.5 24 3656.0 25 4509.0 26 5718.0 27 7307.0 28 8955.0 29 10752.5 30 13295.5 31 15976.0 32 18858.0 33 22027.5 34 25495.5 35 29033.5 36 33167.0 37 37109.5 38 39507.0 39 41845.5 40 44302.5 41 48475.5 42 52545.5 43 56128.5 44 60603.0 45 63851.5 46 65882.0 47 66618.5 48 68245.5 49 68675.5 50 66889.5 51 65123.0 52 64504.0 53 65174.0 54 65112.5 55 62701.0 56 57607.0 57 52465.0 58 48671.5 59 44202.0 60 38351.0 61 32267.0 62 26805.5 63 22210.0 64 17691.0 65 13711.5 66 10585.0 67 8578.0 68 6533.0 69 4415.5 70 3293.5 71 2520.5 72 2010.0 73 1714.5 74 1372.0 75 1048.5 76 729.5 77 427.5 78 250.5 79 165.5 80 108.5 81 80.5 82 55.5 83 32.0 84 26.5 85 26.5 86 27.0 87 21.5 88 15.5 89 16.5 90 15.5 91 17.0 92 17.5 93 13.0 94 11.5 95 12.5 96 12.5 97 15.5 98 12.5 99 5.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04965897752223092 2 0.012776058244951146 3 0.0110995619141657 4 0.00554978095708285 5 0.009423065583380255 6 0.0063591240133240995 7 0.005781021830294636 8 0.010521459731136237 9 0.014799415885554265 10-11 0.002572554714481113 12-13 0.009394160474228782 14-15 0.008960583836956686 16-17 0.02084058369821216 18-19 0.012429196935133467 20-21 0.004249051045266557 22-23 0.010665985276893603 24-25 0.022979561775421178 26-27 0.008122335671563964 28-29 0.018961751603366404 30-31 0.01500175164961458 32-33 0.013151824663920297 34-35 0.2634411648065265 36-37 0.4667597025779889 38-39 0.4632332792615092 40-41 0.48231065130148143 42-43 0.4732922572462218 44-45 0.4876870016036554 46-47 0.4945375124725546 48-49 0.5008966364858788 50-51 0.5111001400163487 52-53 0.5004052496303036 54-55 0.48725342496638335 56-57 0.48294656370281386 58-59 0.4801716732242724 60-61 0.478986563749062 62-63 0.47583590685155147 64-65 0.490895468719469 66-67 0.4655167828844755 68-69 0.4911845198109837 70-71 0.4734078776828277 72-73 0.48124116226287694 74-75 0.49395941028952517 76-77 0.48106773160796806 78-79 0.4807208702981504 80-81 0.4841027680688728 82-83 0.4836691914316007 84-85 0.48442072426953897 86-87 0.4858081695088097 88-89 0.4988154686269727 90-91 0.5231824756416645 92-93 0.511793862635984 94-95 0.4829176585936624 96-97 0.45733663699460864 98-99 0.44918539621389314 100-101 0.456440578610913 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1729798.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.856107004301364 #Duplication Level Percentage of deduplicated Percentage of total 1 79.15070890747576 46.585025929247024 2 12.154656608219929 14.307515398678616 3 3.6757093321745233 6.490138253135186 4 1.5666385405845917 3.68824982326837 5 0.8615754004067875 2.535448697930784 6 0.5526221967004189 1.951511468517116 7 0.34428623046010626 1.41843430540473 8 0.2666066748003661 1.2553144784089059 9 0.18425393230269513 0.9760022240013652 >10 1.1132771432445818 12.08789749533975 >50 0.08816245659890465 3.5930492896064883 >100 0.03933923538285437 3.8317876675716893 >500 0.001573339380730582 0.6504036816094987 >1k 5.900022677739683E-4 0.6292212872805187 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3422 0.19782656703268242 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2220 0.12833868463254092 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.7810218302946355E-5 0.0 0.0 0.0 0.0 8 5.7810218302946355E-5 0.0 0.0 0.0 0.0 9 5.7810218302946355E-5 0.0 0.0 0.0 0.0 10-11 8.671532745441954E-5 0.0 0.0 0.0 0.0 12-13 1.1562043660589271E-4 0.0 0.0 0.0 0.0 14-15 1.1562043660589271E-4 0.0 0.0 0.0 0.0 16-17 1.1562043660589271E-4 0.0 0.0 5.7810218302946355E-5 0.0 18-19 1.1562043660589271E-4 0.0 0.0 5.7810218302946355E-5 0.0 20-21 1.1562043660589271E-4 0.0 0.0 8.671532745441954E-5 0.0 22-23 1.1562043660589271E-4 0.0 0.0 3.1795620066620494E-4 0.0 24-25 1.7343065490883907E-4 0.0 0.0 6.070072921809367E-4 0.0 26-27 1.7343065490883907E-4 0.0 0.0 6.359124013324099E-4 0.0 28-29 2.0233576406031225E-4 0.0 0.0 8.382481653927221E-4 0.0 30-31 2.3124087321178542E-4 0.0 0.0 0.0015608758941795518 0.0 32-33 2.3124087321178542E-4 0.0 0.0 0.0038443795171459326 0.0 34-35 2.3124087321178542E-4 0.0 0.0 0.006388029122475572 0.0 36-37 2.3124087321178542E-4 0.0 0.0 0.011793284533801057 0.0 38-39 2.3124087321178542E-4 0.0 0.0 0.02075386837075774 0.0 40-41 2.3124087321178542E-4 0.0 0.0 0.03225810181304407 0.0 42-43 2.3124087321178542E-4 0.0 0.0 0.04355999949127008 0.0 44-45 2.3124087321178542E-4 0.0 0.0 0.055815765771494705 0.0 46-47 2.3124087321178542E-4 0.0 0.0 0.07211824733292557 0.0 48-49 2.3124087321178542E-4 0.0 0.0 0.08943240771465802 0.0 50-51 2.3124087321178542E-4 0.0 0.0 0.10952145857493187 0.0 52-53 2.3124087321178542E-4 0.0 0.0 0.12987065541756898 0.0 54-55 2.3124087321178542E-4 0.0 0.0 0.15319707850280784 0.0 56-57 2.3124087321178542E-4 0.0 0.0 0.1825646694007046 0.0 58-59 2.3124087321178542E-4 5.7810218302946355E-5 0.0 0.21462043544968834 0.0 60-61 2.3124087321178542E-4 5.7810218302946355E-5 0.0 0.24985576350533414 0.0 62-63 2.3124087321178542E-4 5.7810218302946355E-5 0.0 0.28737459518394637 0.0 64-65 2.3124087321178542E-4 5.7810218302946355E-5 0.0 0.3244887553344379 0.0 66-67 2.3124087321178542E-4 5.7810218302946355E-5 0.0 0.36926276941006986 0.0 68-69 2.890510915147318E-4 5.7810218302946355E-5 0.0 0.42160992208338777 0.0 70-71 2.890510915147318E-4 5.7810218302946355E-5 0.0 0.4805763447523931 5.7810218302946355E-5 72-73 2.890510915147318E-4 5.7810218302946355E-5 0.0 0.5434738622659987 5.7810218302946355E-5 74-75 2.890510915147318E-4 5.7810218302946355E-5 0.0 0.608857219166631 5.7810218302946355E-5 76-77 2.890510915147318E-4 5.7810218302946355E-5 0.0 0.6782005760210151 5.7810218302946355E-5 78-79 2.890510915147318E-4 5.7810218302946355E-5 0.0 0.7645112319473141 5.7810218302946355E-5 80-81 3.4686130981767814E-4 5.7810218302946355E-5 0.0 0.8666618876886203 5.7810218302946355E-5 82-83 3.4686130981767814E-4 5.7810218302946355E-5 0.0 0.9680899157011397 5.7810218302946355E-5 84-85 3.4686130981767814E-4 5.7810218302946355E-5 0.0 1.0665696225802088 5.7810218302946355E-5 86-87 3.4686130981767814E-4 5.7810218302946355E-5 0.0 1.1790683073977424 5.7810218302946355E-5 88-89 3.4686130981767814E-4 5.7810218302946355E-5 0.0 1.3087944372695541 5.7810218302946355E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2150 0.0 48.010956 1 GTATCAA 3825 0.0 44.812534 1 ATCAACG 4750 0.0 35.677853 3 TCAACGC 4810 0.0 35.13439 4 CAACGCA 4975 0.0 33.96913 5 TATCAAC 5040 0.0 33.905758 2 AACGCAG 5050 0.0 33.74293 6 GTACATG 6185 0.0 30.85231 1 GTACCTG 1690 0.0 29.698978 1 TACATGG 6250 0.0 29.386541 2 ACGCAGA 6045 0.0 27.79737 7 CGCAGAG 6120 0.0 27.302029 8 ACATGGG 6485 0.0 27.300524 3 TACCTGG 1900 0.0 25.412264 2 CATGGGG 4290 0.0 24.496552 4 GCAGAGT 6875 0.0 23.684126 9 AGTACAT 4230 0.0 23.27661 2 TAGCCCT 1155 0.0 22.541876 4 GAGTACA 4775 0.0 22.4108 1 ATGGGGA 2075 0.0 21.444654 5 >>END_MODULE