FastQCFastQC Report
Thu 26 May 2016
SRR936346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936346_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729798
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35220.20360758886297706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25260.1460286114332425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.045.1194881
GTATCAA40600.040.6585121
ATCAACG48650.033.254513
TCAACGC49300.032.816064
TATCAAC50950.032.0382352
CAACGCA50700.031.9098995
AACGCAG52150.031.1136386
GTACATG62800.029.3156411
TACATGG62650.028.2515372
ACATGGG62350.026.9367873
ACGCAGA61650.026.1652477
CGCAGAG62500.025.7334928
TACCTGG15350.024.421542
CATGGGG39850.023.692114
GTACCTG16550.022.9953521
GCAGAGT71250.022.5732369
GTGTAGC8600.021.5731831
AGTACAT42600.019.9387422
TGCACCG6200.019.8957655
GGACCGT2151.0917756E-619.8601746
Produced by FastQC (version 0.11.5)