##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936346_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1729798 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47216033317185 34.0 31.0 34.0 31.0 34.0 2 32.60692809218186 34.0 31.0 34.0 31.0 34.0 3 32.69338096124518 34.0 31.0 34.0 31.0 34.0 4 36.17664894976176 37.0 35.0 37.0 35.0 37.0 5 36.13679921008118 37.0 35.0 37.0 35.0 37.0 6 36.171710800914326 37.0 37.0 37.0 35.0 37.0 7 36.123551998557055 37.0 36.0 37.0 35.0 37.0 8 36.09927748789165 37.0 36.0 37.0 35.0 37.0 9 37.86785740300313 39.0 38.0 39.0 35.0 39.0 10-11 37.80385628842212 39.0 38.0 39.0 35.0 39.0 12-13 37.74296651979017 39.0 38.0 39.0 35.0 39.0 14-15 39.09915955504631 40.0 38.0 41.0 36.0 41.0 16-17 39.025063909196334 40.0 38.0 41.0 35.5 41.0 18-19 39.03215086385809 40.0 38.5 41.0 35.0 41.0 20-21 38.98360820165129 40.0 38.5 41.0 35.0 41.0 22-23 38.86656968039043 40.0 38.0 41.0 35.0 41.0 24-25 39.01972253407623 40.0 39.0 41.0 35.0 41.0 26-27 38.9426482167282 40.0 38.0 41.0 35.0 41.0 28-29 38.85038917838962 40.0 38.0 41.0 35.0 41.0 30-31 38.739070400127645 40.0 38.0 41.0 35.0 41.0 32-33 38.59269088066931 40.0 38.0 41.0 34.0 41.0 34-35 38.45769506034809 40.0 38.0 41.0 34.0 41.0 36-37 38.31950522546563 40.0 38.0 41.0 34.0 41.0 38-39 38.1892278751623 40.0 38.0 41.0 33.5 41.0 40-41 38.04392536007094 40.0 38.0 41.0 33.0 41.0 42-43 38.07638088377949 40.0 38.0 41.0 33.0 41.0 44-45 37.95639028372099 40.0 38.0 41.0 33.0 41.0 46-47 37.762175410076786 40.0 37.0 41.0 33.0 41.0 48-49 37.549549427158546 40.0 37.0 41.0 33.0 41.0 50-51 37.35078460028281 40.0 37.0 41.0 32.0 41.0 52-53 37.13580342907091 40.0 36.0 41.0 31.5 41.0 54-55 36.93741783722724 39.5 36.0 41.0 31.0 41.0 56-57 36.68363155697949 39.0 35.0 41.0 31.0 41.0 58-59 36.42389776147273 39.0 35.0 41.0 31.0 41.0 60-61 36.143997738464265 38.5 35.0 40.5 30.0 41.0 62-63 35.83699715226865 38.0 35.0 40.0 30.0 41.0 64-65 35.46221148365301 37.0 35.0 40.0 29.0 41.0 66-67 35.10927692135151 37.0 34.0 40.0 29.0 41.0 68-69 34.754636090456806 36.0 34.0 39.0 29.0 41.0 70-71 34.38187869335032 36.0 34.0 39.0 28.0 40.5 72-73 33.97007107188238 35.0 34.0 38.5 28.0 40.0 74-75 33.53357472953489 35.0 33.5 37.0 27.0 39.5 76-77 32.81273767226 35.0 32.5 36.5 26.0 39.0 78-79 32.8705493358184 35.0 33.0 36.0 26.0 39.0 80-81 32.70086189254468 35.0 33.0 36.0 26.0 37.5 82-83 32.41800690022765 35.0 33.0 35.5 26.0 37.0 84-85 32.1249027343077 35.0 33.0 35.0 26.0 37.0 86-87 31.708380111434977 35.0 32.0 35.0 25.0 36.0 88-89 31.219073267514474 35.0 32.0 35.0 23.5 36.0 90-91 30.999746791243833 34.0 32.0 35.0 23.0 36.0 92-93 30.703416815142575 34.0 31.0 35.0 20.0 35.0 94-95 30.53751478496333 34.0 31.0 35.0 20.0 35.0 96-97 30.274080268331907 34.0 31.0 35.0 17.5 35.0 98-99 29.802064171654724 34.0 31.0 35.0 2.0 35.0 100-101 26.389928188146826 31.0 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12690266714044895 1101 2 0.09570900447380382 1101 3 0.20159852493642916 1101 4 0.10040828287093007 1101 5 0.16758570380519444 1101 6 0.16463377715674454 1101 7 0.13773373639561015 1101 8 0.048324732781566127 1101 9 0.21107187863712085 1101 10-11 0.1825119649296596 1101 12-13 0.2523709765533866 1101 14-15 0.2684580846803897 1101 16-17 0.4100584209227236 1101 18-19 0.4299949257243654 1101 20-21 0.23940660524288404 1101 22-23 0.2039543246378699 1101 24-25 0.2462577416485061 1101 26-27 0.37715802288385447 1101 28-29 0.3575760807499435 1101 30-31 0.30498520798848716 1101 32-33 0.450426046480942 1101 34-35 0.3360955274550008 1101 36-37 0.26406401282273606 1101 38-39 0.46602176870009515 1101 40-41 0.4961546473693872 1101 42-43 0.3987183186310901 1101 44-45 0.4716624998228909 1101 46-47 0.5148295333195492 1101 48-49 0.6412305890198482 1101 50-51 0.6059424525707939 1101 52-53 0.6634149724224372 1101 54-55 0.6695044897186904 1101 56-57 0.6054517613708583 1101 58-59 0.532508175686587 1101 60-61 0.6272338627242959 1101 62-63 0.5265701627330657 1101 64-65 0.6604208406492447 1101 66-67 0.5676436910035036 1101 68-69 0.7684027349481894 1101 70-71 0.6787035221039659 1101 72-73 0.7171440192293872 1101 74-75 0.5192457829855925 1101 76-77 0.3738181815734478 1101 78-79 0.395087530632658 1101 80-81 0.6355669346416306 1101 82-83 0.5304581867066602 1101 84-85 0.618572489761803 1101 86-87 0.6524268595305358 1101 88-89 0.6363033957787287 1101 90-91 0.8188993013034604 1101 92-93 0.9601587177246529 1101 94-95 0.8248138339576023 1101 96-97 0.8196388707770907 1101 98-99 0.9848804739252621 1101 100-101 1.2427938893734556 1102 1 0.04313373570973056 1102 2 0.06502594166981623 1102 3 0.13452204135073487 1102 4 0.05907707432195508 1102 5 0.028572811485730654 1102 6 0.108554955962191 1102 7 0.15720847516652015 1102 8 0.11796332910886775 1102 9 0.15068725460110244 1102 10-11 0.15229997097179648 1102 12-13 0.15138986429452217 1102 14-15 0.25007645056894745 1102 16-17 0.29550866584391855 1102 18-19 0.29300862435450625 1102 20-21 0.2253826823857068 1102 22-23 0.2151172288097456 1102 24-25 0.09626189432152898 1102 26-27 0.2072555155344915 1102 28-29 0.1669584398488766 1102 30-31 0.10013255001820909 1102 32-33 0.042099547755285016 1102 34-35 0.17485523649104096 1102 36-37 0.10386599953071851 1102 38-39 0.11663748898988047 1102 40-41 0.05786120565129238 1102 42-43 0.2644187101935458 1102 44-45 0.24610011542725374 1102 46-47 0.19808223558499805 1102 48-49 0.1648482679870824 1102 50-51 0.0033703474597217564 1102 52-53 -0.03380541858633279 1102 54-55 -0.03219494845925652 1102 56-57 0.17424641342011427 1102 58-59 -0.10843527701879907 1102 60-61 0.028114964774502482 1102 62-63 0.09068483879861589 1102 64-65 0.10258037769541062 1102 66-67 0.1683103247948452 1102 68-69 0.06303293941542876 1102 70-71 -0.03640969879574385 1102 72-73 -0.08865774081186117 1102 74-75 -0.10785521002234333 1102 76-77 -0.2288521169518276 1102 78-79 -0.20311950953233548 1102 80-81 -0.09922303526879261 1102 82-83 -0.07362034380078342 1102 84-85 0.15107732156989329 1102 86-87 -0.055045226720487506 1102 88-89 -0.1050170850315979 1102 90-91 0.02298263333908679 1102 92-93 0.050368401758142056 1102 94-95 -0.02742562474147192 1102 96-97 0.03534038702182585 1102 98-99 0.07781080764445036 1102 100-101 0.5766815612984466 1103 1 0.06236288707783899 1103 2 0.12665392385817142 1103 3 0.21134606280316603 1103 4 0.10409911904215363 1103 5 0.15374936582980325 1103 6 0.2109355489987692 1103 7 0.2219830457605667 1103 8 0.19871900072561033 1103 9 0.1355064346279775 1103 10-11 0.23731366199228887 1103 12-13 0.23543924412955164 1103 14-15 0.2835257041684187 1103 16-17 0.31009297416307646 1103 18-19 0.26732480258761626 1103 20-21 0.3216501935539142 1103 22-23 0.31358350476340036 1103 24-25 0.17890771732371746 1103 26-27 0.2873688062453539 1103 28-29 0.3107817963028552 1103 30-31 0.27785962500999517 1103 32-33 0.210147153697406 1103 34-35 0.27034445002995255 1103 36-37 0.3552390120921345 1103 38-39 0.3891270563773048 1103 40-41 0.33227559058689593 1103 42-43 0.49428940117895337 1103 44-45 0.4645322409830186 1103 46-47 0.4770440178316093 1103 48-49 0.4687667280992187 1103 50-51 0.355689534884867 1103 52-53 0.23102715441658717 1103 54-55 0.3466351869205724 1103 56-57 0.4350350939879135 1103 58-59 0.4080150854751281 1103 60-61 0.44050088879050264 1103 62-63 0.44601469790602977 1103 64-65 0.48262615394054365 1103 66-67 0.2779811695796326 1103 68-69 0.32636472122659654 1103 70-71 0.22285388199058076 1103 72-73 0.2750754920741585 1103 74-75 0.2740843110519364 1103 76-77 0.2314001681612936 1103 78-79 0.24047187956193739 1103 80-81 0.1749666537461323 1103 82-83 0.1954787037675345 1103 84-85 0.26404356560530573 1103 86-87 0.1598302350898706 1103 88-89 0.2324654074993262 1103 90-91 0.3172814446701828 1103 92-93 0.45031297817357085 1103 94-95 0.454835657509161 1103 96-97 0.46198403873850324 1103 98-99 0.32321299643214374 1103 100-101 0.9261961716063887 1104 1 0.005744523702148285 1104 2 0.012724228657233994 1104 3 0.064834498094406 1104 4 0.04041313627454457 1104 5 0.0656785521127361 1104 6 0.08542302821589232 1104 7 0.05713437475300509 1104 8 0.05200691573465832 1104 9 0.061272755620187525 1104 10-11 0.11559229212994637 1104 12-13 0.14682924907873485 1104 14-15 0.13988586165300632 1104 16-17 0.20476197570383192 1104 18-19 0.24162135520339945 1104 20-21 0.21103525919857447 1104 22-23 0.25776475204072824 1104 24-25 0.19311212942871236 1104 26-27 0.22998150729061706 1104 28-29 0.2362848057540745 1104 30-31 0.17259430003125686 1104 32-33 0.06307767321375479 1104 34-35 0.23533389698101814 1104 36-37 0.27541052898894947 1104 38-39 0.23572945224469066 1104 40-41 0.19733244035150221 1104 42-43 0.21987039531486374 1104 44-45 0.2565303288347849 1104 46-47 0.12136427054481658 1104 48-49 0.22141075477706806 1104 50-51 0.14608119587629886 1104 52-53 0.23233835942406955 1104 54-55 0.2452466560219122 1104 56-57 0.14195761410376662 1104 58-59 0.10164726951568781 1104 60-61 -0.0795552568885114 1104 62-63 0.03517231508275387 1104 64-65 -0.13786841883596423 1104 66-67 -0.15471339479725543 1104 68-69 0.18178506855901588 1104 70-71 -0.032420377875475026 1104 72-73 -0.29003286710996434 1104 74-75 -0.12350705743516954 1104 76-77 -0.061733926792264526 1104 78-79 -0.1734865966815491 1104 80-81 -0.009225430684843161 1104 82-83 0.19099293741360412 1104 84-85 0.09479291854652416 1104 86-87 0.04344056365491156 1104 88-89 0.1152913600118417 1104 90-91 0.251268891359377 1104 92-93 0.12414182855414779 1104 94-95 0.02526150988505904 1104 96-97 -6.343691057537626E-4 1104 98-99 -0.0694650075222647 1104 100-101 0.19052163702342995 1105 1 -0.04081576486617422 1105 2 0.10092384522238973 1105 3 0.16315369000066937 1105 4 0.04275439328791464 1105 5 0.052383156851583124 1105 6 0.05651018442878808 1105 7 0.11263000750051333 1105 8 0.13674985755690017 1105 9 0.16559151864272792 1105 10-11 0.11418201684421803 1105 12-13 0.15314368165597614 1105 14-15 0.2655783968358776 1105 16-17 0.20090522286567847 1105 18-19 0.14473044209716335 1105 20-21 0.1428727538775334 1105 22-23 0.061234389313924 1105 24-25 0.08043933569938133 1105 26-27 0.054630478769844615 1105 28-29 0.004053474198151719 1105 30-31 0.051820725029422476 1105 32-33 0.029275875102761972 1105 34-35 0.0737023465023583 1105 36-37 0.08562108208891317 1105 38-39 0.10969965122753678 1105 40-41 0.23249450398631666 1105 42-43 0.2165897549338638 1105 44-45 0.060945350647038765 1105 46-47 0.030676168227238065 1105 48-49 0.16513416958763827 1105 50-51 0.23083053894806937 1105 52-53 0.16298402145729796 1105 54-55 0.09843599858158569 1105 56-57 0.26331010210942196 1105 58-59 0.15076245680024414 1105 60-61 0.21354515631214355 1105 62-63 0.22167151972750077 1105 64-65 0.10227085635055744 1105 66-67 0.20513124687679607 1105 68-69 0.14726793081373302 1105 70-71 0.15769633290647533 1105 72-73 0.148793191736722 1105 74-75 0.04883858443412237 1105 76-77 -0.04769278596157989 1105 78-79 -0.1320941168694887 1105 80-81 -0.08936107316225872 1105 82-83 0.017191411355710784 1105 84-85 -0.035044825781163524 1105 86-87 0.001812837922848587 1105 88-89 0.020168792086582954 1105 90-91 0.23776025447345717 1105 92-93 0.1797006736407063 1105 94-95 0.1654020653214765 1105 96-97 -0.10652903974546035 1105 98-99 -0.1148178603991461 1105 100-101 0.2642796345845575 1106 1 -0.06114777205404209 1106 2 -0.10428107956397525 1106 3 -0.020565716451145022 1106 4 -0.11863201725219596 1106 5 -0.0663421740962633 1106 6 -0.0015961740071475106 1106 7 -0.019726372514710988 1106 8 -0.12392875226598932 1106 9 -0.08859800245397054 1106 10-11 -0.03195666344487336 1106 12-13 -0.016239425290983434 1106 14-15 -0.004435806260552511 1106 16-17 -0.04914813683362951 1106 18-19 0.02501050204835309 1106 20-21 0.08101728803951147 1106 22-23 0.05207731562261131 1106 24-25 -0.017531230148840393 1106 26-27 -0.14388055020918245 1106 28-29 -0.018511989521179828 1106 30-31 0.047407760191894965 1106 32-33 0.09894794836249332 1106 34-35 -0.05439898984423053 1106 36-37 -0.029683146667252913 1106 38-39 0.030002035935275728 1106 40-41 -0.07788081872215002 1106 42-43 -0.05640906016693492 1106 44-45 -0.03253319169152746 1106 46-47 0.047648476112705396 1106 48-49 0.026912146156455208 1106 50-51 0.02525523507242866 1106 52-53 0.0999540537085366 1106 54-55 0.18235413067822748 1106 56-57 0.30069994964193114 1106 58-59 0.29496469006059556 1106 60-61 0.14321942673596766 1106 62-63 0.20312882804454802 1106 64-65 0.28429785792781104 1106 66-67 0.2838238557737398 1106 68-69 0.19824213767672916 1106 70-71 0.26515707821592116 1106 72-73 0.09815267916972914 1106 74-75 0.25843469859248813 1106 76-77 0.1645552267819781 1106 78-79 0.024184475689970952 1106 80-81 0.11837059032636432 1106 82-83 0.02188294521165801 1106 84-85 0.16292142420902422 1106 86-87 0.1620765368485948 1106 88-89 0.2431421043899178 1106 90-91 0.2902985675222567 1106 92-93 0.33798476695740476 1106 94-95 0.5319076720134142 1106 96-97 0.6773592736956928 1106 98-99 0.45956791453278 1106 100-101 0.6405865548667329 1107 1 0.07761850190660624 1107 2 0.09018450538533784 1107 3 0.13407115732481856 1107 4 0.14604372122791887 1107 5 0.09478094482161481 1107 6 0.08731368626257563 1107 7 0.14638725854458556 1107 8 0.13914977879025514 1107 9 0.09265155359543087 1107 10-11 0.13668773973331128 1107 12-13 0.15701699042944028 1107 14-15 0.17009106924047757 1107 16-17 0.19342444274769122 1107 18-19 0.217617075561499 1107 20-21 0.14493196834434485 1107 22-23 0.20976654005359308 1107 24-25 0.21821922394920534 1107 26-27 0.3558698227093302 1107 28-29 0.305645011706666 1107 30-31 0.04068406489928833 1107 32-33 0.09073765276750123 1107 34-35 0.14760310052889736 1107 36-37 0.23322998107889958 1107 38-39 0.22757578247246357 1107 40-41 0.11465878167156518 1107 42-43 0.1476357103486592 1107 44-45 0.06402073689077525 1107 46-47 0.1361849105044186 1107 48-49 -0.10225972107441095 1107 50-51 -0.0687953194852966 1107 52-53 -0.09684950195526199 1107 54-55 0.01706041295977201 1107 56-57 -0.05234681991980494 1107 58-59 -0.16656128231805667 1107 60-61 0.05486564630631818 1107 62-63 0.035997408518845475 1107 64-65 0.05879023212489187 1107 66-67 0.011531447075356027 1107 68-69 0.0690682409573995 1107 70-71 0.1803386666965423 1107 72-73 0.12023351369538915 1107 74-75 0.15766044874217044 1107 76-77 -0.06355318849195157 1107 78-79 0.06567026419697441 1107 80-81 0.2002655237084099 1107 82-83 0.20080569273314808 1107 84-85 0.08388604007759426 1107 86-87 0.09974993126952825 1107 88-89 0.45110480239188533 1107 90-91 0.2499015995780276 1107 92-93 0.3740315263716951 1107 94-95 0.3353273600810347 1107 96-97 0.09902913680669201 1107 98-99 0.13131642032189816 1107 100-101 0.12453837481051622 1108 1 -0.22513754950848153 1108 2 -0.10601592762486689 1108 3 -0.12650150841861318 1108 4 -0.06228489840577822 1108 5 -0.08363096100841716 1108 6 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-0.6745138571214895 2316 52-53 -0.6461777428935349 2316 54-55 -0.8223837448846467 2316 56-57 -0.6224134383187305 2316 58-59 -0.4781315646545963 2316 60-61 -0.7208601909580636 2316 62-63 -0.6203934828534798 2316 64-65 -0.6843572605048678 2316 66-67 -0.571618899991492 2316 68-69 -0.527374268152272 2316 70-71 -0.5315371296096387 2316 72-73 -0.508458336440718 2316 74-75 -0.4597993401186855 2316 76-77 -0.4858405440789966 2316 78-79 -0.6459001668081612 2316 80-81 -0.5441273657698673 2316 82-83 -0.572586642211256 2316 84-85 -0.49000210464236105 2316 86-87 -0.7143894931153909 2316 88-89 -0.6783192405911791 2316 90-91 -0.728280837605773 2316 92-93 -0.7042035394959285 2316 94-95 -0.47028339282134723 2316 96-97 -0.512229684816262 2316 98-99 -0.478037213212982 2316 100-101 -0.34987769210224684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 13.0 5 36.0 6 52.0 7 118.0 8 298.0 9 533.0 10 955.0 11 1392.0 12 2068.0 13 3204.0 14 3967.0 15 5089.0 16 6563.0 17 8717.0 18 8201.0 19 5677.0 20 5485.0 21 5601.0 22 6295.0 23 7112.0 24 8438.0 25 10158.0 26 12762.0 27 15891.0 28 20363.0 29 24765.0 30 31089.0 31 38700.0 32 48663.0 33 63045.0 34 88112.0 35 130570.0 36 222808.0 37 405574.0 38 455096.0 39 82309.0 40 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.990140147857204 17.920991341183345 12.845225246322409 24.24364326463704 2 16.300321609597635 18.885688083791962 39.65541167994468 25.15857862666573 3 20.43065144022597 22.77439331066402 27.931874126343075 28.863081122766935 4 13.218595466060199 14.870522454066892 35.36927433145373 36.54160774841918 5 14.455040415123616 36.80140686947262 33.57016252764773 15.173390187756027 6 35.19694207069265 34.223186753597815 16.320807400632905 14.259063775076628 7 31.192717299939066 30.626408401443406 19.283523278440605 18.897351020176924 8 27.473323474764104 35.557099730720005 18.370179639472354 18.599397155043537 9 26.833190927495583 15.972963317104078 19.105352185630924 38.08849356976942 10-11 26.456369402172925 25.64569272735209 26.30088805603225 21.59704981444274 12-13 26.50386909674057 22.38650615838337 27.416519804497234 23.693104940378827 14-15 23.035088490101156 24.318880007954686 24.9686090514615 27.67742245048266 16-17 23.560887864744316 27.60906595336256 25.53635830466175 23.293687877231374 18-19 23.288990968887695 26.266592977908402 27.387070629056108 23.05734542414779 20-21 23.850602122676822 25.6930437502204 27.471171472721945 22.985182654380832 22-23 23.52126391746081 25.534766661858782 27.44912479589362 23.49484462478679 24-25 23.398862844738126 25.683377125662304 27.622669106318526 23.29509092328104 26-27 23.40365629286186 26.07839538201164 27.376958908980015 23.140989416146482 28-29 23.092654413182355 26.070946569956117 27.63586877188337 23.20053024497815 30-31 23.37794889805192 25.693477918402802 27.579703169916364 23.34887001362892 32-33 23.188594311897806 25.85152290609986 27.7279878781467 23.231894903855633 34-35 23.62322577362976 25.904572173840656 27.335399891777396 23.136802160752193 36-37 23.624137290137888 25.96949988047727 27.223288982258897 23.18307384712595 38-39 23.36882310841638 26.019803514175678 27.40944875516969 23.201924622238256 40-41 23.514471137503556 25.892479309603257 27.384743101466768 23.208306451426417 42-43 23.57744704603339 25.798656266991415 27.154905654541583 23.46899103243361 44-45 23.43754606930211 26.046940246778256 27.178965977946863 23.33654770597278 46-47 23.394334555901793 26.314969366406572 26.893615151407403 23.39708092628424 48-49 23.75550181623175 26.197159075304597 26.81333358388129 23.23400552458237 50-51 23.54525106688285 26.30346446773509 26.780110985301686 23.371173480080373 52-53 23.67426262647286 26.359891297152434 26.578025455151984 23.387820621222723 54-55 23.539030392653917 26.097139946802884 26.74820050722832 23.615629153314877 56-57 23.43306970079668 26.487857881818893 26.80091007712993 23.278162340254493 58-59 23.613361722349183 26.287405833237464 26.750297870765998 23.348934573647355 60-61 23.70979868231684 26.091895580850384 27.040485241318468 23.15782049551431 62-63 23.52020293699033 26.282982174797286 26.890798810034468 23.306016078177915 64-65 23.445278269651787 26.17886446765743 26.77391158061232 23.601945682078462 66-67 23.391777537030336 26.437104216792946 26.63230041889284 23.53881782728388 68-69 23.13914688304646 26.292896627236246 26.93580406498331 23.632152424733988 70-71 23.259252322023034 26.139217034614788 26.960585803350746 23.640944840011436 72-73 23.444354676698815 26.2810571910468 26.692585296621374 23.58200283563301 74-75 23.29578366953829 26.424385968766295 26.560008740905005 23.719821620790405 76-77 23.43647368878334 26.472211764024443 26.436270960641778 23.655043586550438 78-79 23.436060481201476 26.36475062615451 26.549558798338897 23.649630094305117 80-81 23.126618161447745 26.681117448784658 26.651720638381942 23.540543751385652 82-83 23.435366441630755 26.65074187853148 26.51156377796714 23.402327901870624 84-85 23.467627479715684 26.366658284122014 26.332636714967467 23.83307752119483 86-87 23.201853798353415 26.336114686843054 26.77438623461535 23.687645280188182 88-89 23.467315761562293 26.402082189741634 26.523525813384442 23.60707623531163 90-91 23.461925098430704 26.61176564054403 26.499816026858632 23.42649323416664 92-93 23.395246226231855 26.419218024798013 26.564776617857238 23.620759131112894 94-95 23.247122715573756 26.492956231977228 26.634103307056023 23.62581774539299 96-97 23.247663821550884 26.449344066303297 26.524094839248257 23.778897272897556 98-99 23.232732879432657 26.23915520593474 26.48705273083289 24.041059183799714 100-101 23.169035458163876 26.412415415788665 26.434730327757492 23.98381879828996 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 78.0 1 58.0 2 96.5 3 182.0 4 150.0 5 114.5 6 261.5 7 552.0 8 861.5 9 965.0 10 871.5 11 816.5 12 861.5 13 968.0 14 1074.5 15 1160.5 16 1238.5 17 1311.5 18 1398.0 19 1518.5 20 1699.5 21 1966.5 22 2349.5 23 2828.0 24 3585.0 25 4608.0 26 5928.0 27 7499.5 28 9041.5 29 10969.5 30 13562.5 31 16457.0 32 19309.0 33 22336.5 34 26161.0 35 29579.5 36 33410.5 37 37154.5 38 39764.5 39 42136.0 40 44348.0 41 48667.5 42 52914.5 43 56598.0 44 60981.0 45 63986.5 46 65964.0 47 67045.0 48 68659.5 49 68690.5 50 67213.5 51 65839.0 52 64743.5 53 65186.0 54 65616.5 55 63192.0 56 57488.0 57 52639.5 58 49242.5 59 44351.5 60 38127.5 61 31838.5 62 26368.5 63 21802.5 64 17550.0 65 13597.0 66 10306.0 67 8453.5 68 6354.0 69 4250.5 70 3105.0 71 2359.0 72 1905.5 73 1686.0 74 1371.0 75 1007.0 76 665.0 77 348.0 78 194.5 79 104.0 80 69.5 81 44.0 82 26.5 83 15.5 84 8.0 85 5.5 86 4.0 87 4.0 88 6.0 89 4.5 90 1.5 91 1.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26708320855961215 2 0.021505401208696047 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.91386855575044E-4 12-13 8.671532745441954E-5 14-15 0.0 16-17 4.3357663727209764E-4 18-19 0.0 20-21 5.781021830294635E-5 22-23 8.671532745441954E-5 24-25 0.0024858393870266933 26-27 0.0023413138412693275 28-29 0.0018210218765428103 30-31 0.0011851094752104003 32-33 0.0017054014399369175 34-35 0.0017343065490883906 36-37 8.671532745441954E-5 38-39 2.312408732117854E-4 40-41 5.781021830294635E-4 42-43 0.0037287590805400402 44-45 0.0038732846262974057 46-47 0.014018977938464492 48-49 0.014770510776402793 50-51 0.006330218904172625 52-53 0.007428613051928607 54-55 2.8905109151473174E-4 56-57 0.0031506568975105767 58-59 0.0012140145843618734 60-61 0.0011272992569074539 62-63 0.0 64-65 0.0010983941477559808 66-67 0.0 68-69 0.0 70-71 1.4452554575736587E-4 72-73 0.001474160566725132 74-75 0.0 76-77 0.19354861087826442 78-79 0.10348029076227398 80-81 0.0010694890386045075 82-83 0.0 84-85 7.515328379383026E-4 86-87 0.12151707887279324 88-89 0.5821488983106698 90-91 0.3099494854312469 92-93 0.2926931352678174 94-95 0.0024280291687237467 96-97 0.002890510915147318 98-99 0.002948321133450264 100-101 7.515328379383026E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1729798.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.82648037431244 #Duplication Level Percentage of deduplicated Percentage of total 1 77.84942956737298 44.239090814617384 2 12.743719489702446 14.48361450954638 3 3.950720237008783 6.7351657803833564 4 1.71275391229835 3.8931910633299607 5 0.9320762956067313 2.648330765982888 6 0.5700129300241975 1.9435097148674634 7 0.3829986040877764 1.523512386100816 8 0.2873928577548738 1.306521967273992 9 0.21829812203186688 1.116460255265383 >10 1.2073468628947688 12.714177781967937 >50 0.0986618758633057 3.845977522724685 >100 0.04414821308602407 4.143643385072409 >500 0.0018307742009002568 0.7276194010495863 >1k 6.102580669667522E-4 0.6791846518177528 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3522 0.20360758886297706 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2526 0.1460286114332425 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.7810218302946355E-5 0.0 0.0 0.0 0.0 7 5.7810218302946355E-5 0.0 0.0 0.0 0.0 8 5.7810218302946355E-5 0.0 0.0 0.0 0.0 9 5.7810218302946355E-5 0.0 0.0 0.0 0.0 10-11 5.7810218302946355E-5 0.0 0.0 0.0 0.0 12-13 5.7810218302946355E-5 0.0 0.0 0.0 0.0 14-15 5.7810218302946355E-5 0.0 0.0 0.0 0.0 16-17 5.7810218302946355E-5 0.0 0.0 5.7810218302946355E-5 0.0 18-19 5.7810218302946355E-5 0.0 0.0 5.7810218302946355E-5 0.0 20-21 5.7810218302946355E-5 0.0 0.0 8.671532745441954E-5 0.0 22-23 5.7810218302946355E-5 0.0 0.0 3.757664189691513E-4 2.8905109151473177E-5 24-25 1.1562043660589271E-4 0.0 0.0 6.648175104838831E-4 5.7810218302946355E-5 26-27 1.1562043660589271E-4 0.0 0.0 6.937226196353563E-4 5.7810218302946355E-5 28-29 1.445255457573659E-4 0.0 0.0 8.960583836956685E-4 5.7810218302946355E-5 30-31 1.7343065490883907E-4 0.0 0.0 0.001618686112482498 5.7810218302946355E-5 32-33 1.7343065490883907E-4 0.0 0.0 0.0037865692988429864 5.7810218302946355E-5 34-35 1.7343065490883907E-4 0.0 0.0 0.006214598467566734 5.7810218302946355E-5 36-37 1.7343065490883907E-4 0.0 0.0 0.01173547431549811 1.1562043660589271E-4 38-39 1.7343065490883907E-4 0.0 0.0 0.020782773479909214 1.1562043660589271E-4 40-41 1.7343065490883907E-4 0.0 0.0 0.0325471529045588 1.1562043660589271E-4 42-43 1.7343065490883907E-4 0.0 0.0 0.0439646710193907 1.1562043660589271E-4 44-45 1.7343065490883907E-4 0.0 0.0 0.056336057736221225 1.1562043660589271E-4 46-47 2.3124087321178542E-4 0.0 0.0 0.07266744440680356 1.1562043660589271E-4 48-49 2.3124087321178542E-4 0.0 0.0 0.08969255369702128 1.1562043660589271E-4 50-51 2.3124087321178542E-4 0.0 0.0 0.10998394032135544 1.1562043660589271E-4 52-53 2.3124087321178542E-4 0.0 0.0 0.13044875760059846 1.1562043660589271E-4 54-55 2.3124087321178542E-4 0.0 0.0 0.1540064215590491 1.1562043660589271E-4 56-57 2.3124087321178542E-4 0.0 0.0 0.18346072778440026 1.1562043660589271E-4 58-59 2.3124087321178542E-4 0.0 0.0 0.2156610193791414 1.1562043660589271E-4 60-61 2.3124087321178542E-4 0.0 0.0 0.25127211385375636 1.1562043660589271E-4 62-63 2.890510915147318E-4 0.0 0.0 0.28928233238794354 1.1562043660589271E-4 64-65 2.890510915147318E-4 0.0 0.0 0.3266566385207984 1.1562043660589271E-4 66-67 2.890510915147318E-4 0.0 0.0 0.37195094456115685 1.1562043660589271E-4 68-69 2.890510915147318E-4 0.0 0.0 0.42464495854429246 1.1562043660589271E-4 70-71 2.890510915147318E-4 0.0 0.0 0.4837270016499036 1.1562043660589271E-4 72-73 2.890510915147318E-4 0.0 0.0 0.5466245191635093 1.1562043660589271E-4 74-75 2.890510915147318E-4 0.0 0.0 0.6123547373739593 1.1562043660589271E-4 76-77 2.890510915147318E-4 0.0 0.0 0.6815246635734346 1.1562043660589271E-4 78-79 3.17956200666205E-4 0.0 0.0 0.7679509399363393 1.445255457573659E-4 80-81 3.4686130981767814E-4 0.0 0.0 0.8699570701318882 1.7343065490883907E-4 82-83 3.4686130981767814E-4 0.0 0.0 0.9715585287993165 1.7343065490883907E-4 84-85 3.757664189691513E-4 0.0 0.0 1.0703272867699003 1.7343065490883907E-4 86-87 4.335766372720977E-4 0.0 0.0 1.1823923949501618 1.7343065490883907E-4 88-89 4.6248174642357084E-4 0.0 0.0 1.3119450941670645 1.7343065490883907E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2425 0.0 45.119488 1 GTATCAA 4060 0.0 40.658512 1 ATCAACG 4865 0.0 33.25451 3 TCAACGC 4930 0.0 32.81606 4 TATCAAC 5095 0.0 32.038235 2 CAACGCA 5070 0.0 31.909899 5 AACGCAG 5215 0.0 31.113638 6 GTACATG 6280 0.0 29.315641 1 TACATGG 6265 0.0 28.251537 2 ACATGGG 6235 0.0 26.936787 3 ACGCAGA 6165 0.0 26.165247 7 CGCAGAG 6250 0.0 25.733492 8 TACCTGG 1535 0.0 24.42154 2 CATGGGG 3985 0.0 23.69211 4 GTACCTG 1655 0.0 22.995352 1 GCAGAGT 7125 0.0 22.573236 9 GTGTAGC 860 0.0 21.573183 1 AGTACAT 4260 0.0 19.938742 2 TGCACCG 620 0.0 19.895765 5 GGACCGT 215 1.0917756E-6 19.860174 6 >>END_MODULE