FastQCFastQC Report
Thu 26 May 2016
SRR936345_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936345_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141807
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60040.2803240441365632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40240.18787873977440545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28210.13171121394224594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79100.033.033571
TACCTGG19550.029.7720852
GTACCTG20550.029.7146641
GTACATG99100.029.4238831
GGTATCA56450.029.349251
TACATGG103850.027.1575552
TCAACGC95900.027.041654
ATCAACG96350.026.8662363
CAACGCA98050.026.545225
TATCAAC98700.026.2732832
ACATGGG105000.026.2303983
AACGCAG100700.026.0346346
CATGGGG50600.024.7837224
TATCACG1000.001690926623.6603372
ACGCAGA117250.022.1580077
CGCAGAG119550.021.6144738
CATGGGA53550.021.2975084
AGTACAT70050.020.1982612
ATGGGGA29600.019.8244515
GGACCGT2151.1190641E-619.809586
Produced by FastQC (version 0.11.5)