##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936345_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2141807 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.693178236881288 33.0 31.0 34.0 30.0 34.0 2 31.780724873903203 33.0 31.0 34.0 30.0 34.0 3 31.84778460430842 34.0 31.0 34.0 30.0 34.0 4 35.34105033740202 37.0 35.0 37.0 33.0 37.0 5 35.32496718892038 37.0 35.0 37.0 33.0 37.0 6 35.4245233113908 37.0 35.0 37.0 33.0 37.0 7 35.364192478594006 37.0 35.0 37.0 33.0 37.0 8 35.369558975201784 37.0 35.0 37.0 33.0 37.0 9 37.02830086931269 39.0 37.0 39.0 33.0 39.0 10-11 36.9468820019731 39.0 37.0 39.0 33.0 39.0 12-13 36.84648430040615 39.0 37.0 39.0 33.0 39.0 14-15 38.08247755283273 40.0 38.0 41.0 33.0 41.0 16-17 38.032081321986524 40.0 38.0 41.0 33.0 41.0 18-19 37.98669557994721 40.0 38.0 41.0 33.0 41.0 20-21 37.913642779204665 40.0 38.0 41.0 33.0 41.0 22-23 37.75031200290222 40.0 38.0 41.0 32.5 41.0 24-25 37.899858857497435 40.0 38.0 41.0 33.0 41.0 26-27 37.854466578921446 40.0 38.0 41.0 33.0 41.0 28-29 37.71625011030406 40.0 38.0 41.0 32.5 41.0 30-31 37.566785662760466 40.0 37.5 41.0 32.0 41.0 32-33 37.38471556961015 40.0 37.0 41.0 32.0 41.0 34-35 37.121004833768865 40.0 37.0 41.0 31.0 41.0 36-37 36.92247434059185 40.0 37.0 41.0 31.0 41.0 38-39 36.76568406957303 40.0 36.0 41.0 30.0 41.0 40-41 36.57494512810912 40.0 36.0 41.0 30.0 41.0 42-43 36.38398137647323 40.0 36.0 41.0 30.0 41.0 44-45 36.19313131388589 40.0 35.5 41.0 29.5 41.0 46-47 35.89638352101753 39.0 35.0 41.0 28.5 41.0 48-49 35.71281025788038 39.0 35.0 41.0 27.5 41.0 50-51 35.39723420457585 38.5 35.0 40.5 27.5 41.0 52-53 35.40833674556111 39.0 35.0 40.5 27.5 41.0 54-55 35.47980350236973 39.0 35.0 41.0 27.0 41.0 56-57 35.32100954941318 39.0 35.0 41.0 27.0 41.0 58-59 35.083765017109386 39.0 35.0 41.0 26.5 41.0 60-61 34.7918185438744 38.0 34.5 40.5 26.0 41.0 62-63 34.487910675425 37.5 34.0 40.0 26.0 41.0 64-65 34.14988418657704 37.0 34.0 40.0 25.5 41.0 66-67 33.79589710931003 36.5 34.0 39.5 25.0 41.0 68-69 33.42382156748951 36.0 33.5 39.0 23.5 41.0 70-71 33.02392045595144 35.5 33.0 39.0 23.0 40.5 72-73 32.62371025960789 35.0 33.0 38.0 22.0 40.0 74-75 32.213396678598954 35.0 33.0 37.0 20.5 39.5 76-77 31.819437512343548 35.0 32.0 37.0 20.0 39.0 78-79 31.438324975126143 35.0 32.0 36.0 18.5 39.0 80-81 31.05511327584605 35.0 32.0 36.0 17.5 37.5 82-83 30.725827070319596 35.0 31.0 35.0 13.5 37.0 84-85 30.42582174771116 35.0 31.0 35.0 8.5 36.5 86-87 30.108575375839187 34.0 31.0 35.0 6.5 36.0 88-89 29.800355961111343 34.0 31.0 35.0 2.0 36.0 90-91 29.467537691304585 34.0 30.0 35.0 2.0 35.5 92-93 29.170131575814253 34.0 30.0 35.0 2.0 35.0 94-95 28.80984304374764 34.0 29.5 35.0 2.0 35.0 96-97 28.40391547884567 34.0 29.0 35.0 2.0 35.0 98-99 27.921392777220355 34.0 29.0 35.0 2.0 35.0 100-101 24.68442814875477 30.0 22.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3748204647405764 1101 2 0.29603426337784455 1101 3 0.3713831784884576 1101 4 0.38281569846716224 1101 5 0.48699475744344056 1101 6 0.35853819739509163 1101 7 0.4880885704959681 1101 8 0.431518660835728 1101 9 0.4869183321957493 1101 10-11 0.5818256448800412 1101 12-13 0.47488978963904316 1101 14-15 0.6398244893178315 1101 16-17 0.6599548610179156 1101 18-19 0.7173693131187875 1101 20-21 0.6424370403643564 1101 22-23 0.7114017141591447 1101 24-25 0.6229425217354105 1101 26-27 0.4867435145264025 1101 28-29 0.4603341275079771 1101 30-31 0.7069665367132245 1101 32-33 0.7266589174734364 1101 34-35 0.5143187290731888 1101 36-37 0.5636659812215044 1101 38-39 0.5636925603500345 1101 40-41 0.49646325749564113 1101 42-43 0.4598150360543656 1101 44-45 0.6098156941509458 1101 46-47 0.5822173634261603 1101 48-49 0.599893133762869 1101 50-51 0.5586433178015966 1101 52-53 0.5741446890229369 1101 54-55 0.44023789402122304 1101 56-57 0.6655187114823917 1101 58-59 0.6857578022761217 1101 60-61 0.404255404908902 1101 62-63 0.4019092925398482 1101 64-65 0.35632363865291694 1101 66-67 0.47994155515802817 1101 68-69 0.6741343538754663 1101 70-71 0.7125398334623796 1101 72-73 0.7121904812728062 1101 74-75 0.683345428382097 1101 76-77 0.6198862376389691 1101 78-79 0.7621588982289715 1101 80-81 0.8013680699331225 1101 82-83 0.9085031928324092 1101 84-85 0.8518656985897444 1101 86-87 0.718895309984152 1101 88-89 0.6074532251713514 1101 90-91 0.7213160960329326 1101 92-93 0.7744325364610738 1101 94-95 0.9558591302897064 1101 96-97 0.9919029164798694 1101 98-99 0.9980405557107339 1101 100-101 1.1829128895304812 1102 1 0.2832014032424688 1102 2 0.2711799934856849 1102 3 0.24273263928706257 1102 4 0.13904566057733803 1102 5 0.31345205852183966 1102 6 0.3166801385201339 1102 7 0.2906796518133703 1102 8 0.27325430642010673 1102 9 0.2579136688323018 1102 10-11 0.36651596840088274 1102 12-13 0.45885437722284195 1102 14-15 0.582656753963704 1102 16-17 0.535896277514567 1102 18-19 0.4448263227369722 1102 20-21 0.4151957113057776 1102 22-23 0.5169095835849689 1102 24-25 0.5234700647257995 1102 26-27 0.5140314772194969 1102 28-29 0.5301250048032315 1102 30-31 0.6645299293101345 1102 32-33 0.26426384315107754 1102 34-35 0.3720365955844116 1102 36-37 0.3454876075695097 1102 38-39 0.32938769296443127 1102 40-41 0.32925908961455264 1102 42-43 0.3141956830960382 1102 44-45 0.4658583930725442 1102 46-47 0.5895118839741045 1102 48-49 0.5994807175576824 1102 50-51 0.49382037988262084 1102 52-53 0.5816264727594529 1102 54-55 0.5151504559565225 1102 56-57 0.6339783442425215 1102 58-59 0.6474293266130431 1102 60-61 0.6094506832534066 1102 62-63 0.652849251735411 1102 64-65 0.5287915780291925 1102 66-67 0.6928947757934125 1102 68-69 0.6366095797571347 1102 70-71 0.6920581954559637 1102 72-73 0.5442000994599212 1102 74-75 0.5407113566828841 1102 76-77 0.6582613469365519 1102 78-79 0.68557991251053 1102 80-81 0.5114919405247846 1102 82-83 0.5067537028877886 1102 84-85 0.5922074356926323 1102 86-87 0.5777731881537811 1102 88-89 0.5694154810734204 1102 90-91 0.5767241403348855 1102 92-93 0.689213941299311 1102 94-95 0.6264318496135175 1102 96-97 0.7072679704000073 1102 98-99 0.5954050267104449 1102 100-101 0.48019648032033757 1103 1 0.20964996355202814 1103 2 0.2268386920543115 1103 3 0.2157327612477431 1103 4 0.18241737456844476 1103 5 0.153975990534299 1103 6 0.13217829247886215 1103 7 0.18415087463554158 1103 8 0.1694932052259972 1103 9 0.24816041747132545 1103 10-11 0.300968891576737 1103 12-13 0.3269313066963022 1103 14-15 0.33917375084816115 1103 16-17 0.42748403931425827 1103 18-19 0.30341983886349766 1103 20-21 0.39704324936305113 1103 22-23 0.3359680193837562 1103 24-25 0.48388550716589407 1103 26-27 0.3978508977953723 1103 28-29 0.47186895943385565 1103 30-31 0.3174985632395959 1103 32-33 0.40008771529193865 1103 34-35 0.23835493514513217 1103 36-37 0.28539148037559414 1103 38-39 0.3229293203634498 1103 40-41 0.3376085457807747 1103 42-43 0.16253091975520562 1103 44-45 0.26648219425445774 1103 46-47 0.4548128219626406 1103 48-49 0.383848961212955 1103 50-51 0.4409161910874815 1103 52-53 0.5410041406494202 1103 54-55 0.5126671701139358 1103 56-57 0.44393726566658387 1103 58-59 0.5679084657923639 1103 60-61 0.5000023336032129 1103 62-63 0.4771768048714691 1103 64-65 0.4415956481898533 1103 66-67 0.5576865279249503 1103 68-69 0.5968793623344979 1103 70-71 0.5410962305584732 1103 72-73 0.5702201342741589 1103 74-75 0.5485707526448778 1103 76-77 0.4095982023466185 1103 78-79 0.3390878066929979 1103 80-81 0.40850806150978514 1103 82-83 0.5390714825380911 1103 84-85 0.5798318910273004 1103 86-87 0.5883244504468657 1103 88-89 0.6863481975813528 1103 90-91 0.700398095076384 1103 92-93 0.7054588272660709 1103 94-95 0.5685135521846725 1103 96-97 0.6763058946291629 1103 98-99 0.4629062463172886 1103 100-101 0.7691268851189363 1104 1 0.2894100428972415 1104 2 0.19610658756205268 1104 3 0.28608217382677026 1104 4 0.22805340156564569 1104 5 0.2287530228087462 1104 6 0.2291555308525517 1104 7 0.29101275899714096 1104 8 0.21361004709699927 1104 9 0.2062878944554143 1104 10-11 0.26214372807433506 1104 12-13 0.4232773899799014 1104 14-15 0.437910977584842 1104 16-17 0.36556478303669593 1104 18-19 0.34627191893765996 1104 20-21 0.3808887299575545 1104 22-23 0.5094506797800946 1104 24-25 0.5944986374254668 1104 26-27 0.4173667198483173 1104 28-29 0.5013853344625616 1104 30-31 0.4377823678326749 1104 32-33 0.5189828152457139 1104 34-35 0.43277944128738 1104 36-37 0.48218111559410204 1104 38-39 0.3784918070327947 1104 40-41 0.45064509911283324 1104 42-43 0.5982243336504851 1104 44-45 0.6579999274787909 1104 46-47 0.6209219728397599 1104 48-49 0.5745190888359488 1104 50-51 0.5962871247747898 1104 52-53 0.6023753244016277 1104 54-55 0.6821286343773991 1104 56-57 0.756175572612797 1104 58-59 0.7078215478095657 1104 60-61 0.7109085137747044 1104 62-63 0.6632931907417614 1104 64-65 0.6991796562398704 1104 66-67 0.6974341258173311 1104 68-69 0.5827205950945284 1104 70-71 0.6093988156248074 1104 72-73 0.7108234825832298 1104 74-75 0.6420786810369705 1104 76-77 0.7300965506379917 1104 78-79 0.7026452604657258 1104 80-81 0.6252324728289516 1104 82-83 0.7230549619105986 1104 84-85 0.6375154347138867 1104 86-87 0.4558031288729403 1104 88-89 0.6169579119217019 1104 90-91 0.7126215833618765 1104 92-93 0.5330119749103268 1104 94-95 0.3153378232986057 1104 96-97 0.39844660816401856 1104 98-99 0.6496727192063574 1104 100-101 0.9223948765121257 1105 1 0.18339258973448125 1105 2 0.21226021068651946 1105 3 0.2128718511768568 1105 4 0.12170880458106126 1105 5 0.1634382671341541 1105 6 0.1539679945272283 1105 7 0.2469804230494006 1105 8 0.23600863583416043 1105 9 0.16062462297998792 1105 10-11 0.254288821541536 1105 12-13 0.21687515083856823 1105 14-15 0.2544891967159302 1105 16-17 0.3034943586187424 1105 18-19 0.24301911011163924 1105 20-21 0.2771798863644577 1105 22-23 0.39190160621365777 1105 24-25 0.3177323066105089 1105 26-27 0.3202351679030073 1105 28-29 0.2833999185394518 1105 30-31 0.369189795831133 1105 32-33 0.34641684102017933 1105 34-35 0.17812481719848705 1105 36-37 0.18006937697749947 1105 38-39 0.15804457405563 1105 40-41 0.194033857324321 1105 42-43 0.11558023025177278 1105 44-45 0.21117594653446048 1105 46-47 0.35789647980531214 1105 48-49 0.319784074002861 1105 50-51 0.2724887537968925 1105 52-53 0.3593952966515417 1105 54-55 0.46516424734419815 1105 56-57 0.5140973828475879 1105 58-59 0.5888897534429773 1105 60-61 0.4200795164479274 1105 62-63 0.28051937255879267 1105 64-65 0.33327553675238164 1105 66-67 0.3471134531182045 1105 68-69 0.38573842376843004 1105 70-71 0.41587876138961377 1105 72-73 0.5222823393976128 1105 74-75 0.5307541099847057 1105 76-77 0.5827518768503417 1105 78-79 0.5796148669178187 1105 80-81 0.5883502624103656 1105 82-83 0.6444689567034025 1105 84-85 0.42920202189691636 1105 86-87 0.4201019315690715 1105 88-89 0.4700029671468222 1105 90-91 0.5028544932862395 1105 92-93 0.2820356592194635 1105 94-95 0.36063422065052464 1105 96-97 0.285791669953273 1105 98-99 0.3066916550136156 1105 100-101 0.4531058681990565 1106 1 0.14851964474731005 1106 2 0.11365071548075534 1106 3 0.09624760122832399 1106 4 0.10127276394977969 1106 5 0.24148341294316822 1106 6 0.022603796856223823 1106 7 0.018439043727617843 1106 8 0.2839547900276287 1106 9 0.28960197150868794 1106 10-11 0.18114683067415172 1106 12-13 0.24983277501938517 1106 14-15 0.24957354251085206 1106 16-17 0.2242237748189595 1106 18-19 0.12260146125370142 1106 20-21 0.2107132674330714 1106 22-23 0.22846238541264086 1106 24-25 0.22442607031984352 1106 26-27 0.2884096198677 1106 28-29 0.23952265722500243 1106 30-31 0.22763270391487112 1106 32-33 0.2731410570166375 1106 34-35 0.13397945943767553 1106 36-37 0.1657281302499456 1106 38-39 0.16123532990649636 1106 40-41 0.17464813745220198 1106 42-43 0.11188440212327322 1106 44-45 0.19966221175709364 1106 46-47 0.2789687329455006 1106 48-49 0.3731745502273398 1106 50-51 0.3280528264242406 1106 52-53 0.3010118748582826 1106 54-55 0.3304206569792498 1106 56-57 0.37075787045660746 1106 58-59 0.2582098776907529 1106 60-61 0.32829296101279937 1106 62-63 0.31209837899998405 1106 64-65 0.3814595595816499 1106 66-67 0.378145680555825 1106 68-69 0.4114255580214845 1106 70-71 0.4015201687454564 1106 72-73 0.4321459418230873 1106 74-75 0.4235356833154569 1106 76-77 0.4367442818897409 1106 78-79 0.5111968323683236 1106 80-81 0.5255894200008235 1106 82-83 0.5221732297424175 1106 84-85 0.5530884714454238 1106 86-87 0.5118888696347099 1106 88-89 0.3869693397917011 1106 90-91 0.5076918357795783 1106 92-93 0.4135974217788636 1106 94-95 0.404444521625134 1106 96-97 0.5151013713458745 1106 98-99 0.6152698995099222 1106 100-101 0.8165492469183242 1107 1 0.12125015088588498 1107 2 0.1998360667418133 1107 3 0.2337798607611603 1107 4 0.09310123109533919 1107 5 0.20655287547129575 1107 6 0.2901337699053528 1107 7 0.16312556257734911 1107 8 0.2575818941748409 1107 9 0.23670215972016706 1107 10-11 0.2831007156851939 1107 12-13 0.29819812441738236 1107 14-15 0.3465875376965357 1107 16-17 0.09556725336518213 1107 18-19 0.34565337184371714 1107 20-21 0.47116572656747024 1107 22-23 0.46826966976865236 1107 24-25 0.4171544170015835 1107 26-27 0.3543537682689575 1107 28-29 0.43395501647393786 1107 30-31 0.3942273886870993 1107 32-33 0.3810797601992917 1107 34-35 0.3041938807985147 1107 36-37 0.3861779747478806 1107 38-39 0.3603936180923668 1107 40-41 0.40116298583503607 1107 42-43 -0.02320444258848653 1107 44-45 0.2099938630429179 1107 46-47 0.3657777067589123 1107 48-49 0.24850217370276084 1107 50-51 0.29878940762299067 1107 52-53 0.3952385564294403 1107 54-55 0.5312268541969374 1107 56-57 0.4446996836805326 1107 58-59 0.5586376936118782 1107 60-61 0.6711662771128246 1107 62-63 0.6476176330982 1107 64-65 0.38695770604786617 1107 66-67 0.39126199906300485 1107 68-69 0.5389089333795454 1107 70-71 0.6497675948634551 1107 72-73 0.5143926661176152 1107 74-75 0.48723595494125505 1107 76-77 0.3601174381476149 1107 78-79 0.39637194949768784 1107 80-81 0.50046985595705 1107 82-83 0.6245223535745623 1107 84-85 0.4146612017398503 1107 86-87 0.13774378695080358 1107 88-89 0.4505796340494541 1107 90-91 0.6955476433423335 1107 92-93 0.21099776390457237 1107 94-95 0.3882675170881562 1107 96-97 0.5922666237800236 1107 98-99 0.41394686170460915 1107 100-101 0.39664432123963067 1108 1 -0.0291818945784037 1108 2 -0.01271973785483027 1108 3 -0.09208912933639368 1108 4 -0.016635561051295156 1108 5 -0.021251592570813216 1108 6 -0.13330982534979796 1108 7 -0.05654934694112512 1108 8 -0.009392195345220955 1108 9 -0.03960637769417019 1108 10-11 -0.11804936423249757 1108 12-13 -0.15532062715188033 1108 14-15 -0.22161310470699647 1108 16-17 -0.10913686744832773 1108 18-19 -0.3053087790139486 1108 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-1.9022759939544827 2316 98-99 -1.8811301977892185 2316 100-101 -1.8194495594573077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18449.0 3 3954.0 4 3415.0 5 2879.0 6 2799.0 7 2993.0 8 3524.0 9 4116.0 10 5021.0 11 5870.0 12 7594.0 13 11043.0 14 11181.0 15 9940.0 16 12013.0 17 15265.0 18 14057.0 19 10037.0 20 9655.0 21 9946.0 22 10434.0 23 11585.0 24 13190.0 25 15311.0 26 18297.0 27 21833.0 28 26581.0 29 31932.0 30 39183.0 31 48940.0 32 61715.0 33 81395.0 34 114717.0 35 171465.0 36 284674.0 37 470583.0 38 468912.0 39 87188.0 40 121.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.865050092861445 17.782825678336938 12.588844295467538 23.76327993333408 2 15.92824319762118 19.171055586867 39.81145581716565 25.089245398346165 3 20.09694580103091 23.790850710844044 27.979757275954668 28.132446212170382 4 12.939079179230687 14.76295911001458 35.5876038545117 36.710357856243036 5 14.27354741467665 37.406071121898236 33.14000860085719 15.18037286256792 6 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27.56453342416624 22.9983823307278 28-29 22.98186070229772 26.049393208643952 27.999377051201346 22.969369037856982 30-31 23.197397160645014 25.784079672847284 27.925374998686674 23.09314816782103 32-33 23.128081612564458 25.919142969078692 27.89380317744345 23.058972240913402 34-35 23.489950009280946 25.903979525269577 27.542997238186572 23.063073227262905 36-37 23.60274641235637 25.911344630748832 27.382476129090673 23.10343282780413 38-39 23.31183560370217 25.928621470694264 27.700642023515144 23.05890090208842 40-41 23.32436709677389 25.839652160694104 27.80377876902158 23.032201973510425 42-43 23.429488668110686 25.827578134419493 27.515644711292385 23.227288486177436 44-45 23.278020243225573 26.0130821076922 27.47458453011792 23.234313118964316 46-47 23.298230812292857 26.39486066057245 27.06655886585791 23.24034966127678 48-49 23.799794128074677 26.220865802430062 26.77447216282606 23.204867906669204 50-51 23.526166519376794 26.32660618317103 26.844576877570436 23.30265041988174 52-53 23.62430213803741 26.490035303122095 26.671196678700294 23.214465880140196 54-55 23.487388777465313 26.17887616999643 26.780281960430386 23.55345309210787 56-57 23.402911153447256 26.68938930619003 26.80084425185956 23.106855288503155 58-59 23.592122798650554 26.24290281724404 26.76875646731255 23.396217916792857 60-61 23.584937085378098 26.1960815591191 27.153125087967872 23.06585626753493 62-63 23.5117512428218 26.28507410485515 26.938850094567755 23.264324557755295 64-65 23.417126077635253 26.291719434996065 26.87876758833524 23.41238689903344 66-67 23.422187685517187 26.573508257105388 26.629144355738905 23.37515970163852 68-69 23.03061897932356 26.431559928686237 27.064153996055175 23.473667095935028 70-71 23.15786411930036 26.376772703197325 27.01421634192031 23.451146835581998 72-73 23.40139182289388 26.467812482772292 26.670334091450247 23.46046160288358 74-75 23.24709524808348 26.682799354504787 26.549533404940316 23.52057199247141 76-77 23.395269457917177 26.65062841257646 26.52530951445378 23.428792615052576 78-79 23.354919681659034 26.483950993789186 26.747655548973952 23.413473775577827 80-81 23.098403079217793 26.891075274694725 26.642379689023777 23.368141957063706 82-83 23.38305598161488 26.93604668348591 26.435762583112847 23.245134751786363 84-85 23.348885525294676 26.560020606967143 26.36108267278745 23.730011194950727 86-87 23.217422679831913 26.349173355955237 26.835902382994536 23.597501581218317 88-89 23.499863209054574 26.490798403933574 26.60016077042162 23.40917761659023 90-91 23.342393070884917 26.779656402745193 26.654872814380365 23.223077711989525 92-93 23.344705797046693 26.586949129911485 26.595889721440734 23.47245535160108 94-95 23.25503869767673 26.58845101144614 26.631804232991662 23.524706057885467 96-97 23.352453304920353 26.564650747611452 26.46685018149393 23.61604576597427 98-99 23.36704941703327 26.491878661497037 26.299061216841768 23.84201070462792 100-101 23.33571929798228 26.657643467460197 26.245501690501293 23.761135544056227 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 149.0 1 125.0 2 199.0 3 343.5 4 301.5 5 283.0 6 602.0 7 1152.5 8 1864.0 9 2172.5 10 1930.5 11 1811.0 12 1982.0 13 2262.0 14 2507.5 15 2665.0 16 2810.0 17 2964.5 18 3005.5 19 3064.5 20 3163.5 21 3455.5 22 3967.5 23 4562.0 24 5361.0 25 6509.0 26 8305.5 27 10551.5 28 12506.5 29 14524.0 30 17324.0 31 20782.5 32 24608.5 33 28498.0 34 33090.5 35 36918.5 36 41506.0 37 46043.0 38 48636.0 39 51077.5 40 53087.0 41 57759.5 42 63366.5 43 68092.5 44 73402.5 45 77665.0 46 80263.5 47 81398.0 48 85079.0 49 84602.5 50 80022.0 51 78211.0 52 78622.5 53 80270.0 54 80270.5 55 76796.0 56 69842.0 57 63719.0 58 60263.0 59 55492.5 60 48027.0 61 40562.5 62 34063.5 63 28073.0 64 22434.5 65 17249.5 66 12614.0 67 10293.5 68 7610.0 69 4641.0 70 3447.0 71 2707.5 72 2051.0 73 1736.0 74 1456.0 75 1131.5 76 744.5 77 379.5 78 205.5 79 125.0 80 84.5 81 57.5 82 49.0 83 34.5 84 23.0 85 22.5 86 18.0 87 17.5 88 17.5 89 16.5 90 20.0 91 18.0 92 17.0 93 19.0 94 18.5 95 20.5 96 20.0 97 14.5 98 10.5 99 10.0 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045895825347475286 2 0.011205491437837303 3 0.009244530436215774 4 0.005089160694684442 5 0.007470327625224869 6 0.005602745718918651 7 0.005322608432972719 8 0.009851494555765295 9 0.014473759773873184 10-11 0.0024745460258557375 12-13 0.00910446179324281 14-15 0.009337909531531087 16-17 0.019025990670494587 18-19 0.011275525759323786 20-21 0.0036417847172971235 22-23 0.009921528877251779 24-25 0.021944087399098054 26-27 0.0067933291841888645 28-29 0.017695338562251407 30-31 0.0139835195234678 32-33 0.012792936058197589 34-35 0.2666206618990413 36-37 0.47065398516299556 38-39 0.46836619732777046 40-41 0.4892130803569135 42-43 0.4788480007769141 44-45 0.493765311253535 46-47 0.5003251926994355 48-49 0.5075387278125433 50-51 0.5176937044280834 52-53 0.5058345593230389 54-55 0.4925980725620936 56-57 0.4880224968916434 58-59 0.4862949836283101 60-61 0.4830967496137607 62-63 0.4793382410273194 64-65 0.49659002888682313 66-67 0.4714477074731757 68-69 0.49778061235209337 70-71 0.4783811053003375 72-73 0.4869486372955173 74-75 0.5009788463666428 76-77 0.4876489805103822 78-79 0.4876489805103822 80-81 0.48960994151200365 82-83 0.4910806622632198 84-85 0.4897266653811478 86-87 0.4920611427640305 88-89 0.5050875265605165 90-91 0.528455645163173 92-93 0.5171100850823628 94-95 0.48918973558308476 96-97 0.46304358889479774 98-99 0.4546861598640774 100-101 0.46199307407250045 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2141807.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.129189624267205 #Duplication Level Percentage of deduplicated Percentage of total 1 70.90162398428951 31.997328334554926 2 14.190641798708164 12.808243292479057 3 5.575069197558314 7.547950649550608 4 2.863905014322672 5.1698284982903004 5 1.607403751417048 3.627041435022921 6 1.0604272434244089 2.8713733290743457 7 0.7010819277532616 2.2147481481806617 8 0.4871084924104752 1.7586249217266605 9 0.37175791592703655 1.5099420133974255 >10 1.9863171563228392 16.435457901485 >50 0.16101447185872628 4.9862224677758675 >100 0.08991275328938728 7.096452208513473 >500 0.002179504085613266 0.671793699127237 >1k 0.0014530027237421757 1.023779312244519 >5k 1.0378590883872682E-4 0.28121378857700113 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6004 0.2803240441365632 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4024 0.18787873977440545 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2821 0.13171121394224594 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.668954765765543E-5 7 0.0 0.0 0.0 0.0 4.668954765765543E-5 8 0.0 0.0 0.0 0.0 9.337909531531086E-5 9 0.0 0.0 0.0 0.0 1.400686429729663E-4 10-11 0.0 0.0 0.0 0.0 1.400686429729663E-4 12-13 0.0 0.0 0.0 0.0 1.400686429729663E-4 14-15 0.0 0.0 0.0 2.3344773828827716E-5 2.101029644594494E-4 16-17 0.0 0.0 0.0 1.1672386914413858E-4 2.3344773828827713E-4 18-19 0.0 0.0 0.0 2.5679251211710484E-4 2.3344773828827713E-4 20-21 0.0 0.0 0.0 5.369297980630374E-4 2.3344773828827713E-4 22-23 0.0 0.0 0.0 0.0014940655250449738 3.034820597747603E-4 24-25 0.0 0.0 0.0 0.002124374418423322 3.7351638126124345E-4 26-27 0.0 0.0 0.0 0.0025679251211710484 3.7351638126124345E-4 28-29 0.0 0.0 0.0 0.003011475823918775 3.7351638126124345E-4 30-31 0.0 0.0 0.0 0.00490240250405382 3.968611550900712E-4 32-33 0.0 2.3344773828827716E-5 0.0 0.008007257423287905 5.135850242342097E-4 34-35 0.0 4.668954765765543E-5 0.0 0.012279351033963378 5.135850242342097E-4 36-37 0.0 4.668954765765543E-5 0.0 0.02133712327954853 5.135850242342097E-4 38-39 0.0 4.668954765765543E-5 0.0 0.034830402552610946 5.135850242342097E-4 40-41 0.0 4.668954765765543E-5 0.0 0.0507048487562138 5.135850242342097E-4 42-43 0.0 4.668954765765543E-5 0.0 0.06657929495981664 5.135850242342097E-4 44-45 0.0 4.668954765765543E-5 0.0 0.08628228407134723 5.135850242342097E-4 46-47 0.0 4.668954765765543E-5 0.0 0.11023402201972446 5.836193457206928E-4 48-49 0.0 4.668954765765543E-5 0.0 0.13747737307796642 7.003432148648314E-4 50-51 0.0 4.668954765765543E-5 0.0 0.16579458373233444 7.003432148648314E-4 52-53 0.0 4.668954765765543E-5 0.0 0.19581596287620687 7.236879886936592E-4 54-55 0.0 4.668954765765543E-5 0.0 0.22861537010570981 7.470327625224869E-4 56-57 0.0 4.668954765765543E-5 0.0 0.2679046244596268 7.470327625224869E-4 58-59 0.0 4.668954765765543E-5 0.0 0.31085900830466984 7.470327625224869E-4 60-61 0.0 4.668954765765543E-5 0.0 0.36179730479917194 7.470327625224869E-4 62-63 0.0 4.668954765765543E-5 0.0 0.4118484998881785 7.470327625224869E-4 64-65 0.0 4.668954765765543E-5 0.0 0.46351048437137427 7.470327625224869E-4 66-67 0.0 4.668954765765543E-5 0.0 0.5249772738626777 7.470327625224869E-4 68-69 0.0 4.668954765765543E-5 0.0 0.5966457295171788 7.470327625224869E-4 70-71 0.0 4.668954765765543E-5 0.0 0.681293879420508 7.470327625224869E-4 72-73 0.0 4.668954765765543E-5 0.0 0.7648681697277112 7.470327625224869E-4 74-75 0.0 4.668954765765543E-5 0.0 0.8482090122966262 7.470327625224869E-4 76-77 0.0 4.668954765765543E-5 0.0 0.943362310422928 7.470327625224869E-4 78-79 0.0 4.668954765765543E-5 0.0 1.0561175680161659 7.470327625224869E-4 80-81 0.0 4.668954765765543E-5 0.0 1.1893695370311144 7.703775363513145E-4 82-83 0.0 4.668954765765543E-5 0.0 1.3208239584612431 7.937223101801423E-4 84-85 0.0 4.668954765765543E-5 0.0 1.4530020678800657 7.937223101801423E-4 86-87 0.0 4.668954765765543E-5 0.0 1.595125050949969 7.937223101801423E-4 88-89 0.0 4.668954765765543E-5 0.0 1.764071179149195 8.404118578377978E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7910 0.0 33.03357 1 TACCTGG 1955 0.0 29.772085 2 GTACCTG 2055 0.0 29.714664 1 GTACATG 9910 0.0 29.423883 1 GGTATCA 5645 0.0 29.34925 1 TACATGG 10385 0.0 27.157555 2 TCAACGC 9590 0.0 27.04165 4 ATCAACG 9635 0.0 26.866236 3 CAACGCA 9805 0.0 26.54522 5 TATCAAC 9870 0.0 26.273283 2 ACATGGG 10500 0.0 26.230398 3 AACGCAG 10070 0.0 26.034634 6 CATGGGG 5060 0.0 24.783722 4 TATCACG 100 0.0016909266 23.660337 2 ACGCAGA 11725 0.0 22.158007 7 CGCAGAG 11955 0.0 21.614473 8 CATGGGA 5355 0.0 21.297508 4 AGTACAT 7005 0.0 20.198261 2 ATGGGGA 2960 0.0 19.824451 5 GGACCGT 215 1.1190641E-6 19.80958 6 >>END_MODULE