FastQCFastQC Report
Thu 26 May 2016
SRR936345_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936345_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141807
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61400.28667382261800434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45350.2117370986274674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29900.13960174749638973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80450.033.1781431
GTACATG100600.031.1202581
TACCTGG20050.030.2866782
GGTATCA57950.029.8035831
TACATGG104550.029.4947952
GTACCTG22200.027.8615761
ACATGGG106600.027.8542883
ATCAACG98950.026.4605083
TCAACGC100250.026.2126224
TATCAAC102550.025.6289082
CAACGCA102650.025.5073415
AACGCAG104000.025.2674546
CATGGGG51000.023.530734
CATGGGA53850.022.9900464
GTGTAGC9750.021.9595381
AGTACAT69350.021.5486532
ACGCAGA121400.021.4505657
CGCAGAG122250.021.3014188
ACCTGGG29950.020.2716033
ATGGGGA26950.019.712625
Produced by FastQC (version 0.11.5)