##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936345_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2141807 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.480785617004706 34.0 31.0 34.0 31.0 34.0 2 32.612515506766016 34.0 31.0 34.0 31.0 34.0 3 32.69792749766903 34.0 31.0 34.0 31.0 34.0 4 36.184077743699596 37.0 35.0 37.0 35.0 37.0 5 36.14462180766054 37.0 35.0 37.0 35.0 37.0 6 36.184116029128674 37.0 37.0 37.0 35.0 37.0 7 36.13240642130687 37.0 36.0 37.0 35.0 37.0 8 36.11302792455156 37.0 36.0 37.0 35.0 37.0 9 37.87515588472724 39.0 38.0 39.0 35.0 39.0 10-11 37.81396409667164 39.0 38.0 39.0 35.0 39.0 12-13 37.75595723610951 39.0 38.0 39.0 35.0 39.0 14-15 39.10402968147923 40.0 38.5 41.0 36.0 41.0 16-17 39.03587344704728 40.0 38.0 41.0 35.5 41.0 18-19 39.06262795854155 40.0 38.5 41.0 36.0 41.0 20-21 39.01725715715749 40.0 38.5 41.0 35.0 41.0 22-23 38.90141268564348 40.0 38.0 41.0 35.0 41.0 24-25 39.04492328206977 40.0 39.0 41.0 35.0 41.0 26-27 38.96811220618851 40.0 38.5 41.0 35.0 41.0 28-29 38.87205126325575 40.0 38.0 41.0 35.0 41.0 30-31 38.75877308272874 40.0 38.0 41.0 35.0 41.0 32-33 38.605099105568335 40.0 38.0 41.0 34.5 41.0 34-35 38.46682964431436 40.0 38.0 41.0 34.0 41.0 36-37 38.32661953201199 40.0 38.0 41.0 34.0 41.0 38-39 38.19557200998969 40.0 38.0 41.0 33.5 41.0 40-41 38.04458921835628 40.0 38.0 41.0 33.0 41.0 42-43 38.062263079726605 40.0 38.0 41.0 33.0 41.0 44-45 37.91891309534426 40.0 38.0 41.0 33.0 41.0 46-47 37.69326741391731 40.0 37.0 41.0 33.0 41.0 48-49 37.456863993814565 40.0 37.0 41.0 33.0 41.0 50-51 37.254229722846176 40.0 37.0 41.0 32.0 41.0 52-53 37.03006830213927 40.0 36.0 41.0 31.5 41.0 54-55 36.830445740442535 39.5 36.0 41.0 31.0 41.0 56-57 36.5802068066824 39.0 35.0 41.0 31.0 41.0 58-59 36.31890828632085 39.0 35.0 41.0 31.0 41.0 60-61 36.037708112822486 38.5 35.0 40.5 30.0 41.0 62-63 35.7418161393627 38.0 35.0 40.0 30.0 41.0 64-65 35.360421130381965 37.0 35.0 40.0 29.0 41.0 66-67 35.01174148744495 37.0 34.0 39.5 29.0 41.0 68-69 34.663598073962774 36.0 34.0 39.0 29.0 41.0 70-71 34.294142282661326 36.0 34.0 39.0 28.0 40.5 72-73 33.89026929130402 35.0 34.0 38.0 28.0 40.0 74-75 33.468259511711366 35.0 33.5 37.0 27.0 39.5 76-77 32.74126356856617 35.0 32.5 36.0 26.0 39.0 78-79 32.791304725402426 35.0 33.0 36.0 26.0 39.0 80-81 32.62704576089256 35.0 33.0 36.0 26.0 37.5 82-83 32.36030300582639 35.0 33.0 35.5 26.0 37.0 84-85 32.084572512836125 35.0 33.0 35.0 26.0 37.0 86-87 31.68502063911454 35.0 32.5 35.0 25.0 36.0 88-89 31.20480253356161 35.0 32.0 35.0 23.5 36.0 90-91 30.996941834628423 34.5 32.0 35.0 23.0 36.0 92-93 30.72077549471077 34.0 31.5 35.0 20.0 35.0 94-95 30.57218017309683 34.0 31.0 35.0 20.0 35.0 96-97 30.33136295660627 34.0 31.0 35.0 18.5 35.0 98-99 29.891574264161058 34.0 31.0 35.0 2.0 35.0 100-101 26.548780305601767 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16175911200003412 1101 2 0.17958073796022944 1101 3 0.23060102965187212 1101 4 0.16927968193136422 1101 5 0.12365716220751466 1101 6 0.1878759027309016 1101 7 0.1622596924310713 1101 8 0.06309272423063561 1101 9 0.155280166744717 1101 10-11 0.20159879793533264 1101 12-13 0.306102300106609 1101 14-15 0.39110049075065234 1101 16-17 0.4171507695022427 1101 18-19 0.35529079992557655 1101 20-21 0.2738530252411451 1101 22-23 0.309168520961542 1101 24-25 0.2331116726015523 1101 26-27 0.2861803017183817 1101 28-29 0.33681829891885684 1101 30-31 0.27008211853904385 1101 32-33 0.4069462021689674 1101 34-35 0.40194421927820656 1101 36-37 0.369448269470702 1101 38-39 0.31211590923519594 1101 40-41 0.30963234268354967 1101 42-43 0.3259631594049708 1101 44-45 0.3433173888236354 1101 46-47 0.3539744801440676 1101 48-49 0.32407257880583984 1101 50-51 0.3082682272040529 1101 52-53 0.4340036977528783 1101 54-55 0.40720409482420195 1101 56-57 0.2986983323567287 1101 58-59 0.3523716778398125 1101 60-61 0.42906199829364056 1101 62-63 0.24980226938300376 1101 64-65 0.20836901719747658 1101 66-67 0.26835719960799054 1101 68-69 0.3950645960975052 1101 70-71 0.5037729346732931 1101 72-73 0.41677159755379023 1101 74-75 0.3147254073889627 1101 76-77 0.22424019139643292 1101 78-79 0.31251072820801795 1101 80-81 0.3284042245212433 1101 82-83 0.40179546526755416 1101 84-85 0.4365795301871387 1101 86-87 0.35813450534979907 1101 88-89 0.3720928241829675 1101 90-91 0.6547294420573841 1101 92-93 0.7690645779405934 1101 94-95 0.6626427547435654 1101 96-97 0.7441875709559334 1101 98-99 0.6963482914468813 1101 100-101 1.1126690047015053 1102 1 0.15964019623203995 1102 2 0.23038808275766343 1102 3 0.180028310328062 1102 4 0.14053441815987355 1102 5 0.1818748829886303 1102 6 0.14270831310688692 1102 7 0.16907986147799647 1102 8 0.22872665019973937 1102 9 0.24895402276337109 1102 10-11 0.2411922109344644 1102 12-13 0.2077191756530894 1102 14-15 0.29772173820037295 1102 16-17 0.4088791285812263 1102 18-19 0.4479809777163055 1102 20-21 0.2644658203446184 1102 22-23 0.3034369558201817 1102 24-25 0.25116617566187927 1102 26-27 0.2821319193225875 1102 28-29 0.2897416448821275 1102 30-31 0.30472143652213646 1102 32-33 0.3495227104755827 1102 34-35 0.21879572029831706 1102 36-37 0.22206412490643856 1102 38-39 0.22801506400057292 1102 40-41 0.2721389005384083 1102 42-43 0.3985243660502604 1102 44-45 0.3152060510212422 1102 46-47 0.4005716821259995 1102 48-49 0.40660245347794444 1102 50-51 0.2509817218120389 1102 52-53 0.30617434770916674 1102 54-55 0.23309231983148493 1102 56-57 0.34687014815387585 1102 58-59 0.16876485767076588 1102 60-61 0.19770306503802715 1102 62-63 0.3285635634663535 1102 64-65 0.37142497755888115 1102 66-67 0.35678477174440104 1102 68-69 0.26908747572442593 1102 70-71 0.24442726285749217 1102 72-73 -0.03750485246078483 1102 74-75 0.0669987970712711 1102 76-77 0.02920230156839665 1102 78-79 0.04773369527301696 1102 80-81 0.18633194238775985 1102 82-83 0.24313108170591136 1102 84-85 0.2894992328977182 1102 86-87 0.28755522817114 1102 88-89 0.40160754292385903 1102 90-91 0.47320874838207416 1102 92-93 0.4721256388557826 1102 94-95 0.4689331948484927 1102 96-97 0.18584015037884072 1102 98-99 0.1480606202140038 1102 100-101 0.7826522457391043 1103 1 0.006209774802435675 1103 2 0.1024446725151833 1103 3 0.1378423868085008 1103 4 -0.020012686382607114 1103 5 0.08948439813947573 1103 6 0.15569989781967308 1103 7 0.12194760065952437 1103 8 0.09429105098472235 1103 9 0.07818550414219061 1103 10-11 0.13436158106296858 1103 12-13 0.22761193340591035 1103 14-15 0.2789834491979022 1103 16-17 0.3518159418594209 1103 18-19 0.18291606137024274 1103 20-21 0.28255161968602494 1103 22-23 0.31193622959974476 1103 24-25 0.16763336269413287 1103 26-27 0.22407475302365043 1103 28-29 0.11809750398428065 1103 30-31 0.19586695224952422 1103 32-33 0.2804752234200478 1103 34-35 0.23920791969295152 1103 36-37 0.2514159038693293 1103 38-39 0.380846769343556 1103 40-41 0.43119337261495616 1103 42-43 0.399983875103068 1103 44-45 0.44183616644040313 1103 46-47 0.46425594808899007 1103 48-49 0.5676149489044207 1103 50-51 0.691340175350561 1103 52-53 0.7137374346449619 1103 54-55 0.6315441120991778 1103 56-57 0.6497421836776951 1103 58-59 0.5816009425110593 1103 60-61 0.6672471261070712 1103 62-63 0.6355258554402354 1103 64-65 0.6511398667409694 1103 66-67 0.5588012807861134 1103 68-69 0.6622522723272724 1103 70-71 0.417515251947556 1103 72-73 0.5144907292362433 1103 74-75 0.5723667302811677 1103 76-77 0.5587458914257013 1103 78-79 0.46566912263434546 1103 80-81 0.7046654050733352 1103 82-83 0.6882261833213335 1103 84-85 0.567376534905641 1103 86-87 0.5578950827052971 1103 88-89 0.5233301267163917 1103 90-91 0.8085137332478602 1103 92-93 0.8949961775893875 1103 94-95 0.9005616194490678 1103 96-97 0.6860402767744596 1103 98-99 0.7426802137523048 1103 100-101 0.9139996063990203 1104 1 -5.899202164485473E-4 1104 2 0.07285229736989152 1104 3 0.051132791426034885 1104 4 0.033855869372736436 1104 5 0.07039992441848852 1104 6 0.029766070222258634 1104 7 0.015116497707154508 1104 8 0.05160649804928141 1104 9 0.04674433763029384 1104 10-11 0.07807396066474581 1104 12-13 0.0543320761880679 1104 14-15 0.12869417205162392 1104 16-17 0.055163787855398994 1104 18-19 0.2112301908270453 1104 20-21 0.17874758230922083 1104 22-23 0.08191261508323322 1104 24-25 0.1325930867183729 1104 26-27 0.22111102087856693 1104 28-29 0.2023140776211747 1104 30-31 0.04211299595313989 1104 32-33 0.09830539557529505 1104 34-35 0.1910656594603779 1104 36-37 0.19060273531005834 1104 38-39 0.3550456831684201 1104 40-41 0.4738350222587684 1104 42-43 0.4527609166391784 1104 44-45 0.4676462418766434 1104 46-47 0.6313735306206212 1104 48-49 0.5874023556033805 1104 50-51 0.5117089965615094 1104 52-53 0.5674534181232431 1104 54-55 0.52198286158945 1104 56-57 0.5261425675463798 1104 58-59 0.5607410897224696 1104 60-61 0.47300989623776957 1104 62-63 0.4125905457043473 1104 64-65 0.32246850949602646 1104 66-67 0.2438878514565559 1104 68-69 0.3965077858088435 1104 70-71 0.3748593012065271 1104 72-73 0.26906456062549466 1104 74-75 0.37339679064269404 1104 76-77 0.3848915387028953 1104 78-79 0.31311223627467655 1104 80-81 0.42900358434480523 1104 82-83 0.40568335562122115 1104 84-85 0.6714893397811963 1104 86-87 0.6434442462000796 1104 88-89 0.6156266840745523 1104 90-91 0.7808589524727871 1104 92-93 0.8131877725212959 1104 94-95 0.6661564211500526 1104 96-97 0.6423009838697382 1104 98-99 0.6052090781335018 1104 100-101 0.7677184500967442 1105 1 -0.0029094257216826236 1105 2 0.04137792580626609 1105 3 0.07632924381571371 1105 4 0.08756007554934797 1105 5 -0.00351571074413215 1105 6 0.014516012487085561 1105 7 0.043626909039353734 1105 8 0.0928901349318636 1105 9 0.09200127043506257 1105 10-11 0.10483730174128425 1105 12-13 0.11341515397627688 1105 14-15 0.1066535546613423 1105 16-17 0.17742070285461153 1105 18-19 0.14157420208866256 1105 20-21 0.08967769391815494 1105 22-23 0.06830704635658691 1105 24-25 0.1377687889946344 1105 26-27 0.1350340599622868 1105 28-29 0.09783800071075177 1105 30-31 0.051001953347636686 1105 32-33 0.16045846965091215 1105 34-35 0.12266961293101275 1105 36-37 0.10966945324963007 1105 38-39 0.046255931938567585 1105 40-41 0.1416215342066991 1105 42-43 0.16780296830664554 1105 44-45 0.06588426279206061 1105 46-47 0.17520283011029392 1105 48-49 0.003646228964974796 1105 50-51 0.0060710142868032335 1105 52-53 0.056762645040670634 1105 54-55 -0.08059346908997611 1105 56-57 0.10427625854988065 1105 58-59 0.12468136298721788 1105 60-61 0.06969072850653646 1105 62-63 0.1903311326125916 1105 64-65 0.183161448477982 1105 66-67 0.16324211579355108 1105 68-69 0.27178639621032374 1105 70-71 0.07628834302060739 1105 72-73 0.0922752154685611 1105 74-75 0.18245110050343527 1105 76-77 0.15161168403873404 1105 78-79 0.02272585311138897 1105 80-81 0.10183292130586352 1105 82-83 0.19459905323616056 1105 84-85 0.20777187511126272 1105 86-87 0.19478150968851438 1105 88-89 0.20507567129734028 1105 90-91 0.2745680113270481 1105 92-93 0.2859447445248513 1105 94-95 0.0646882868451577 1105 96-97 0.08786342807789538 1105 98-99 0.04459086932432399 1105 100-101 0.33580276656680397 1106 1 -0.05897015275537143 1106 2 -0.03661237629583525 1106 3 -0.10410578667100623 1106 4 -0.010457617795758267 1106 5 -0.030632990312604136 1106 6 -0.06093795948548575 1106 7 -0.003556279910483795 1106 8 0.06640266518837024 1106 9 0.014368345232441015 1106 10-11 0.045148344636459115 1106 12-13 0.04663209961034909 1106 14-15 0.13709104673770156 1106 16-17 0.03802368772974063 1106 18-19 0.1452672242600599 1106 20-21 0.11510782800973374 1106 22-23 -0.057138622533848604 1106 24-25 0.05945235815165972 1106 26-27 -0.08772054301901733 1106 28-29 0.0158340320670618 1106 30-31 -0.01731940322548553 1106 32-33 -0.013275807742438417 1106 34-35 0.08250233707405386 1106 36-37 0.0014438155257323615 1106 38-39 0.06226952909649697 1106 40-41 0.030839031849055232 1106 42-43 -0.01699417757583177 1106 44-45 0.016202624133519805 1106 46-47 -0.028092205309810936 1106 48-49 -0.08660355478501458 1106 50-51 -0.006523269060274117 1106 52-53 -0.08453502338754504 1106 54-55 -0.08189377511786233 1106 56-57 0.07790816952655177 1106 58-59 0.17216478934652457 1106 60-61 -0.02571066493176488 1106 62-63 0.020805025605120875 1106 64-65 0.08786629877889851 1106 66-67 0.026168305602254804 1106 68-69 -0.006227322879190922 1106 70-71 -0.05426416778806242 1106 72-73 -0.16329658629516075 1106 74-75 -0.004185987974260286 1106 76-77 0.03735183557571986 1106 78-79 -0.19060634593504489 1106 80-81 -0.11702395217940165 1106 82-83 -0.1357136247667441 1106 84-85 0.12512725544471692 1106 86-87 0.08202029740280992 1106 88-89 0.08967188514290214 1106 90-91 0.26668952151356606 1106 92-93 0.2563410043370915 1106 94-95 0.24199569551572253 1106 96-97 0.27834850408489586 1106 98-99 0.31666322934596636 1106 100-101 0.6427881202360126 1107 1 0.06711169277571827 1107 2 0.12767015475694166 1107 3 0.0759458074103847 1107 4 0.07230118357866644 1107 5 0.09777397626450579 1107 6 0.07975382886234428 1107 7 0.009741051890060248 1107 8 0.13392909207064463 1107 9 0.08052552828451098 1107 10-11 0.10472910832201876 1107 12-13 0.035132991974130334 1107 14-15 0.2035986122465161 1107 16-17 0.15962201393897857 1107 18-19 0.1535817946657616 1107 20-21 0.17541608517988294 1107 22-23 0.11816785626007942 1107 24-25 0.1563607622495482 1107 26-27 0.2040851916091455 1107 28-29 0.19056981617219293 1107 30-31 0.07139259192208414 1107 32-33 0.05638149492661171 1107 34-35 0.14948294536340256 1107 36-37 0.25566004913662255 1107 38-39 0.3726521499149342 1107 40-41 0.31029762214716783 1107 42-43 0.04231308137480028 1107 44-45 0.06583559586368892 1107 46-47 0.1183089116798186 1107 48-49 0.03905711727182393 1107 50-51 0.07503459909006693 1107 52-53 0.15459094704483078 1107 54-55 0.21152956734023576 1107 56-57 0.128622325189518 1107 58-59 0.06733295175462217 1107 60-61 0.13241556167147905 1107 62-63 0.26457554838421515 1107 64-65 0.40824156791708077 1107 66-67 0.09634734468630768 1107 68-69 0.20084301931003523 1107 70-71 0.2795213018196989 1107 72-73 0.21363753015884157 1107 74-75 0.31467699978246344 1107 76-77 0.193686321677923 1107 78-79 0.14834752954898534 1107 80-81 0.3793889941877424 1107 82-83 0.4326353011151909 1107 84-85 0.2277017196559612 1107 86-87 0.26693847621853806 1107 88-89 0.481923325310067 1107 90-91 0.20937671678139225 1107 92-93 0.45740932679904134 1107 94-95 0.4682396855568136 1107 96-97 0.37578314346619024 1107 98-99 0.4662568227456525 1107 100-101 0.7162829576522007 1108 1 -0.10545311404392521 1108 2 -0.10572242790122033 1108 3 -0.038938174144242055 1108 4 -0.035452210626836234 1108 5 -0.04442363784019676 1108 6 -0.1386970539066681 1108 7 0.011033973203282699 1108 8 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-0.619794659341963 2316 74-75 -0.5439829357745225 2316 76-77 -0.4574666910483387 2316 78-79 -0.6665849312989494 2316 80-81 -0.6289573460274553 2316 82-83 -0.7084033631456848 2316 84-85 -0.6217595673798328 2316 86-87 -0.7706132420813887 2316 88-89 -0.7791790050283964 2316 90-91 -0.8267059776106116 2316 92-93 -0.9058724003293328 2316 94-95 -0.7873040871424202 2316 96-97 -0.5902913138781933 2316 98-99 -0.7030478444250576 2316 100-101 -0.6435892463513646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 26.0 5 37.0 6 85.0 7 182.0 8 378.0 9 660.0 10 1172.0 11 1695.0 12 2686.0 13 4008.0 14 5145.0 15 7036.0 16 9207.0 17 12894.0 18 11872.0 19 7342.0 20 6837.0 21 7013.0 22 7539.0 23 8572.0 24 10071.0 25 12382.0 26 14842.0 27 19132.0 28 24118.0 29 30202.0 30 37317.0 31 46688.0 32 58863.0 33 76827.0 34 106842.0 35 160163.0 36 276348.0 37 506154.0 38 564379.0 39 102983.0 40 102.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.132488978173576 18.223080736839 12.956106047362603 23.68832423762482 2 16.147166436675327 19.546181560065097 39.44736700780103 24.859284995458548 3 20.117452226087597 23.839216138522286 28.275096682380813 27.7682349530093 4 13.160616246001625 15.10766376242117 35.475745480335064 36.255974511242144 5 14.199925576861034 37.399214775187495 33.19257991032806 15.208279737623418 6 34.376346701640244 34.555261048264384 16.45587114058363 14.612521109511734 7 30.80795795326096 31.547753835896515 19.35225722952628 18.292030981316245 8 27.712581012201383 35.09153719266022 18.52585223598578 18.670029559152624 9 27.57554718982616 15.88966699613924 19.15555416524458 37.37923164879002 10-11 26.31094603727468 26.004591926659902 26.12572821256862 21.558733823496798 12-13 26.532130609462577 22.890272457109777 27.39266646590142 23.18493046752622 14-15 23.23141160711493 24.451946417207527 25.17596123273479 27.140680742942756 16-17 23.22772570310151 28.059091528791726 25.713910194995226 22.999272573111536 18-19 23.17358660234092 26.344367162867616 27.850571970303577 22.631474264487885 20-21 23.743508108692357 25.70304553655991 27.823148356305765 22.73029799844197 22-23 23.52408503660694 25.457078999181533 27.859022778429615 23.159813185781914 24-25 23.270070395737317 25.8427574846539 27.93770418475741 22.949467934851377 26-27 23.226106777703176 26.136169445847756 27.72878635214623 22.908937424302838 28-29 22.869529184491743 26.046655499851173 28.15916797497388 22.924647340683197 30-31 23.102029782216345 25.758813399032533 28.066565146818196 23.072591671932923 32-33 23.03964436841169 25.921423758980318 28.033534927001863 23.005396945606126 34-35 23.389519027980167 25.945516015514293 27.649809713194763 23.01515524331078 36-37 23.497476076831475 25.889267671255713 27.591874048261637 23.02138220365117 38-39 23.19280849228034 25.9664983350484 27.790591482694026 23.050101689977236 40-41 23.176291491745367 25.838589240387684 27.9426640206014 23.042455247265554 42-43 23.314730748893126 25.8412192961056 27.609394752600412 23.234655202400866 44-45 23.19821639099324 26.020894372526822 27.576953156917906 23.203936079562034 46-47 23.113712393322835 26.43002034080657 27.18329793771492 23.272969328155675 48-49 23.67378749220177 26.19775186877309 26.90750740598232 23.22095323304282 50-51 23.468929882820525 26.303094031205525 26.9222598044892 23.305716281484745 52-53 23.558395732637045 26.465125845697045 26.778855840390392 23.197622581275514 54-55 23.403684611934963 26.177473661750316 26.82949547798475 23.589346248329967 56-57 23.272768785336385 26.70706067084801 26.87384118591592 23.14632935789969 58-59 23.484550835577377 26.216644313790034 26.902054713772287 23.3967501368603 60-61 23.509004069742875 26.147835341747044 27.27432746991231 23.068833118597766 62-63 23.382125466953838 26.26011587411938 27.063479575890824 23.29427908303596 64-65 23.345106187973947 26.231054703504054 26.983018655177222 23.44082045334478 66-67 23.364920368641993 26.511188916648422 26.751780155728316 23.37211055898127 68-69 22.864343721059768 26.407030700485784 27.19445010555342 23.53417547290103 70-71 23.12288364590106 26.334991204837984 27.084500088126706 23.457625061134255 72-73 23.31581747556406 26.44406609755887 26.707212504029954 23.53290392284712 74-75 23.186076056339346 26.61217840823193 26.643133578328953 23.558611957099775 76-77 23.3180216758429 26.69725255627022 26.535621708028728 23.449104059858147 78-79 23.300898950503857 26.474532851643374 26.81086517939165 23.41370301846112 80-81 22.964523966935975 26.887235127351776 26.732667719994176 23.415573185718074 82-83 23.27597678035416 26.875647525664075 26.501127319128194 23.347248374853567 84-85 23.269884965594265 26.523139013369168 26.38409669650769 23.822879324528877 86-87 23.13646340009677 26.30403736072122 26.91818460179184 23.641314637390174 88-89 23.378708790821708 26.446530571604892 26.641932784081142 23.532827853492254 90-91 23.191672017546203 26.755966640485994 26.72807649814089 23.324284843826913 92-93 23.204740048667635 26.615712298563505 26.591081128098242 23.588466524670622 94-95 23.105091779400738 26.63213441617805 26.62389355326399 23.638880251157225 96-97 23.19476725998321 26.55086083970742 26.49693287626077 23.757439024048605 98-99 23.206330737470125 26.473182331570438 26.3834656011161 23.93702132984334 100-101 23.044367701943393 26.612894000801663 26.47452871879165 23.868209578463293 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 135.0 1 108.0 2 202.0 3 353.5 4 292.0 5 258.5 6 565.0 7 1127.0 8 1739.5 9 2055.5 10 1826.5 11 1558.0 12 1583.0 13 1671.5 14 1811.0 15 1955.0 16 2048.0 17 2167.5 18 2278.5 19 2408.5 20 2640.5 21 3044.0 22 3675.0 23 4435.5 24 5432.0 25 6688.5 26 8473.5 27 10681.0 28 12524.0 29 14682.5 30 17727.0 31 21205.0 32 24906.5 33 28767.0 34 33428.0 35 37209.0 36 42083.0 37 46808.0 38 49419.5 39 51883.0 40 53834.5 41 58437.0 42 63885.0 43 68605.0 44 73198.5 45 77233.5 46 80723.0 47 81777.5 48 85782.5 49 85764.5 50 80479.0 51 78382.0 52 78398.5 53 79592.5 54 80368.0 55 77291.5 56 70058.0 57 64060.5 58 60133.5 59 55487.5 60 48374.5 61 40423.0 62 33854.5 63 27704.0 64 21911.5 65 16986.0 66 12323.5 67 9831.5 68 7168.0 69 4380.5 70 3314.5 71 2545.0 72 1942.0 73 1762.0 74 1492.5 75 1140.5 76 725.0 77 331.5 78 160.0 79 73.0 80 38.0 81 19.5 82 11.0 83 5.0 84 2.0 85 1.5 86 1.5 87 1.0 88 1.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2716397882722393 2 0.01970298911153059 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.101029644594494E-4 12-13 9.337909531531086E-5 14-15 0.0 16-17 5.135850242342097E-4 18-19 0.0 20-21 2.3344773828827716E-5 22-23 0.0 24-25 0.0027079937641440147 26-27 0.002287787835225116 28-29 0.002077684870765667 30-31 0.0011905834652702135 32-33 0.0018675819063062172 34-35 0.0018675819063062172 36-37 1.1672386914413858E-4 38-39 1.4006864297296627E-4 40-41 4.90240250405382E-4 42-43 0.0037118190387836064 44-45 0.003828542907927745 46-47 0.013960174749638973 48-49 0.01377341655900835 50-51 0.006303088933783483 52-53 0.007306914208423075 54-55 3.501716074324157E-4 56-57 0.00326826833603588 58-59 0.001143893917612558 60-61 0.0011905834652702135 62-63 0.0 64-65 7.236879886936592E-4 66-67 0.0 68-69 2.3344773828827716E-5 70-71 2.101029644594494E-4 72-73 0.0015640998465314567 74-75 0.0 76-77 0.1970298911153059 78-79 0.10535496428949948 80-81 0.0010738595961260749 82-83 0.0 84-85 6.069641195495205E-4 86-87 0.12428757586467876 88-89 0.5887085064153773 90-91 0.3102053546374627 92-93 0.2942141845647157 94-95 0.0019142714539638725 96-97 0.002848062407116981 98-99 0.0030815101454052586 100-101 5.836193457206929E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2141807.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.15098916169964 #Duplication Level Percentage of deduplicated Percentage of total 1 68.87175226211959 29.71884235409985 2 15.062285137228859 12.999050054139841 3 5.989891468317995 7.754092254874409 4 3.0750848849914103 5.307718181742828 5 1.7694404732244053 3.8176553341189496 6 1.1333789857653023 2.9343854598514016 7 0.7560879622542744 2.2838160425168033 8 0.5181931571375339 1.7888437845846914 9 0.3921474775621413 1.522939639866463 >10 2.1506637962246673 16.967054487431092 >50 0.17838475278619492 5.2859865220298685 >100 0.09867876837281926 7.539977895461174 >500 0.002384844008791347 0.6947718809423034 >1k 0.0015176280055944908 1.0976581807171661 >5k 1.0840200039960649E-4 0.2872079276230888 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6140 0.28667382261800434 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4535 0.2117370986274674 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2990 0.13960174749638973 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.668954765765543E-5 2 0.0 0.0 0.0 0.0 4.668954765765543E-5 3 0.0 0.0 0.0 0.0 4.668954765765543E-5 4 0.0 0.0 0.0 0.0 9.337909531531086E-5 5 0.0 0.0 0.0 0.0 9.337909531531086E-5 6 0.0 0.0 0.0 0.0 9.337909531531086E-5 7 0.0 0.0 0.0 0.0 1.400686429729663E-4 8 0.0 0.0 0.0 0.0 1.400686429729663E-4 9 0.0 0.0 0.0 0.0 1.400686429729663E-4 10-11 0.0 0.0 0.0 0.0 1.400686429729663E-4 12-13 0.0 0.0 0.0 0.0 1.400686429729663E-4 14-15 0.0 0.0 0.0 4.668954765765543E-5 1.400686429729663E-4 16-17 0.0 0.0 0.0 1.6341341680179398E-4 1.400686429729663E-4 18-19 0.0 0.0 0.0 3.0348205977476033E-4 1.400686429729663E-4 20-21 0.0 0.0 0.0 5.836193457206928E-4 1.400686429729663E-4 22-23 0.0 0.0 0.0 0.001540755072702629 1.8675819063062172E-4 24-25 0.0 0.0 0.0 0.002194408739909805 2.5679251211710484E-4 26-27 0.0 0.0 0.0 0.0026613042164863594 2.801372859459326E-4 28-29 0.0 0.0 0.0 0.0031515444668917415 2.801372859459326E-4 30-31 0.0 0.0 0.0 0.005089160694684442 2.801372859459326E-4 32-33 0.0 0.0 0.0 0.008264049935405011 2.801372859459326E-4 34-35 0.0 0.0 0.0 0.012629522641395795 3.7351638126124345E-4 36-37 0.0 0.0 0.0 0.021617260565494463 3.7351638126124345E-4 38-39 0.0 0.0 0.0 0.03536733235067399 3.7351638126124345E-4 40-41 0.0 0.0 0.0 0.05140519197107862 3.7351638126124345E-4 42-43 0.0 0.0 0.0 0.06739636204382561 3.7351638126124345E-4 44-45 0.0 0.0 0.0 0.08719273025067151 3.7351638126124345E-4 46-47 4.668954765765543E-5 0.0 0.0 0.11109777865139109 4.4355070274772657E-4 48-49 4.668954765765543E-5 0.0 0.0 0.138481198352606 4.6689547657655426E-4 50-51 4.668954765765543E-5 0.0 0.0 0.16700851197143346 4.6689547657655426E-4 52-53 4.668954765765543E-5 0.0 0.0 0.19738006272273834 4.902402504053819E-4 54-55 4.668954765765543E-5 0.0 0.0 0.2301327804045836 5.369297980630374E-4 56-57 4.668954765765543E-5 0.0 0.0 0.2697488615921042 5.602745718918652E-4 58-59 4.668954765765543E-5 0.0 0.0 0.3131001065922373 5.602745718918652E-4 60-61 4.668954765765543E-5 0.0 0.0 0.36469205675394656 7.003432148648314E-4 62-63 4.668954765765543E-5 0.0 0.0 0.41577042189142155 7.003432148648314E-4 64-65 4.668954765765543E-5 0.0 0.0 0.4680627152679957 7.236879886936592E-4 66-67 4.668954765765543E-5 0.0 0.0 0.5300430897835333 7.470327625224869E-4 68-69 4.668954765765543E-5 0.0 0.0 0.6019683379501515 7.470327625224869E-4 70-71 4.668954765765543E-5 0.0 0.0 0.6869199699132555 7.470327625224869E-4 72-73 4.668954765765543E-5 0.0 0.0 0.7715681198165848 7.937223101801423E-4 74-75 4.668954765765543E-5 0.0 0.0 0.8553058235405897 7.937223101801423E-4 76-77 4.668954765765543E-5 0.0 0.0 0.950412432119234 7.937223101801423E-4 78-79 4.668954765765543E-5 0.0 0.0 1.0631676897124718 7.937223101801423E-4 80-81 4.668954765765543E-5 0.0 0.0 1.1967698303348526 8.1706708400897E-4 82-83 4.668954765765543E-5 0.0 0.0 1.327920769705207 8.871014054954531E-4 84-85 4.668954765765543E-5 0.0 0.0 1.4597253627427682 8.871014054954531E-4 86-87 4.668954765765543E-5 0.0 0.0 1.602805481539653 8.871014054954531E-4 88-89 4.668954765765543E-5 0.0 0.0 1.772475297727573 9.104461793242808E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8045 0.0 33.178143 1 GTACATG 10060 0.0 31.120258 1 TACCTGG 2005 0.0 30.286678 2 GGTATCA 5795 0.0 29.803583 1 TACATGG 10455 0.0 29.494795 2 GTACCTG 2220 0.0 27.861576 1 ACATGGG 10660 0.0 27.854288 3 ATCAACG 9895 0.0 26.460508 3 TCAACGC 10025 0.0 26.212622 4 TATCAAC 10255 0.0 25.628908 2 CAACGCA 10265 0.0 25.507341 5 AACGCAG 10400 0.0 25.267454 6 CATGGGG 5100 0.0 23.53073 4 CATGGGA 5385 0.0 22.990046 4 GTGTAGC 975 0.0 21.959538 1 AGTACAT 6935 0.0 21.548653 2 ACGCAGA 12140 0.0 21.450565 7 CGCAGAG 12225 0.0 21.301418 8 ACCTGGG 2995 0.0 20.271603 3 ATGGGGA 2695 0.0 19.71262 5 >>END_MODULE