FastQCFastQC Report
Thu 26 May 2016
SRR936344_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936344_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177975
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72300.034.9030271
ATCAACG81300.030.0416743
TCAACGC81650.029.970154
GGTATCA46200.029.5137671
CAACGCA84200.029.0062895
AACGCAG87150.028.1323856
TATCAAC88250.028.0508982
TACATGG120350.026.8617552
GTACATG126800.026.360861
ACATGGG123750.024.900083
GTGTAGC25350.024.4662551
ACGCAGA102150.023.723627
CGCAGAG106150.022.8750658
CATGGGG83100.022.8400964
GAGTACA95650.019.8982681
AGTACAT86900.019.2816092
GTAGCCC23350.018.8521563
GTACCTG33350.018.1713961
TATAGCG2352.6177804E-618.1271365
GCAGAGT132350.018.0244149
Produced by FastQC (version 0.11.5)