##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936344_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4177975 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.773271022445083 33.0 31.0 34.0 30.0 34.0 2 31.87196237411665 34.0 31.0 34.0 30.0 34.0 3 31.947267994662486 34.0 31.0 34.0 30.0 34.0 4 35.42693122864546 37.0 35.0 37.0 33.0 37.0 5 35.406823401288904 37.0 35.0 37.0 33.0 37.0 6 35.48623029099025 37.0 35.0 37.0 33.0 37.0 7 35.431554281679524 37.0 35.0 37.0 33.0 37.0 8 35.430044938038165 37.0 35.0 37.0 33.0 37.0 9 37.113346537497236 39.0 37.0 39.0 34.0 39.0 10-11 37.03133395963356 39.0 37.0 39.0 33.0 39.0 12-13 36.94450505328538 39.0 37.0 39.0 33.0 39.0 14-15 38.225779713856596 40.0 38.0 41.0 33.0 41.0 16-17 38.169341965904536 40.0 38.0 41.0 33.0 41.0 18-19 38.09049982347908 40.0 38.0 41.0 33.0 41.0 20-21 38.00584026950855 40.0 38.0 41.0 33.0 41.0 22-23 37.851946337639646 40.0 38.0 41.0 33.0 41.0 24-25 38.01004900699502 40.0 38.0 41.0 33.0 41.0 26-27 37.97183599710386 40.0 38.0 41.0 33.0 41.0 28-29 37.85116892753068 40.0 38.0 41.0 33.0 41.0 30-31 37.72686995494229 40.0 38.0 41.0 32.5 41.0 32-33 37.5841088086932 40.0 38.0 41.0 32.0 41.0 34-35 37.34525302329478 40.0 37.0 41.0 31.5 41.0 36-37 37.16643708973845 40.0 37.0 41.0 31.0 41.0 38-39 37.022628785476215 40.0 37.0 41.0 31.0 41.0 40-41 36.85581687300666 40.0 37.0 41.0 30.0 41.0 42-43 36.69589131098199 40.0 36.0 41.0 30.0 41.0 44-45 36.54601320017473 40.0 36.0 41.0 30.0 41.0 46-47 36.33125940198302 39.5 36.0 41.0 30.0 41.0 48-49 36.21463615746863 39.0 35.5 41.0 29.5 41.0 50-51 35.92127454089601 39.0 35.5 40.5 28.5 41.0 52-53 35.939846337041274 39.0 35.0 40.5 29.0 41.0 54-55 36.023062727756866 39.0 35.0 41.0 29.0 41.0 56-57 35.86125730766699 39.0 35.0 41.0 29.0 41.0 58-59 35.62477539956558 39.0 35.0 41.0 28.0 41.0 60-61 35.33605765951208 38.0 35.0 40.5 28.0 41.0 62-63 35.033036578725344 38.0 34.5 40.0 28.0 41.0 64-65 34.69648250647742 37.0 34.0 40.0 27.0 41.0 66-67 34.34453724591459 36.5 34.0 39.5 26.5 41.0 68-69 33.96077812816017 36.0 34.0 39.0 26.0 41.0 70-71 33.55774579790449 35.5 34.0 39.0 26.0 40.5 72-73 33.15256075012417 35.0 33.0 38.0 26.0 40.0 74-75 32.74032862810333 35.0 33.0 37.0 25.0 39.5 76-77 32.34940431668451 35.0 33.0 37.0 24.5 39.0 78-79 31.963439225940796 35.0 32.5 36.0 23.5 39.0 80-81 31.588627863977166 35.0 32.0 36.0 22.5 37.0 82-83 31.25428012374416 35.0 32.0 35.0 20.5 37.0 84-85 30.95818871582525 35.0 32.0 35.0 20.0 36.5 86-87 30.64879050257601 34.5 31.0 35.0 18.5 36.0 88-89 30.350792549021953 34.0 31.0 35.0 16.5 36.0 90-91 30.026467008538823 34.0 31.0 35.0 9.0 35.5 92-93 29.745800776692057 34.0 31.0 35.0 4.5 35.0 94-95 29.398540321567268 34.0 30.0 35.0 2.0 35.0 96-97 29.0010509876196 34.0 30.0 35.0 2.0 35.0 98-99 28.541337968752806 34.0 30.0 35.0 2.0 35.0 100-101 25.318300731813856 30.5 23.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2729280283530464 1101 2 0.23088670317772753 1101 3 0.27671509437479713 1101 4 0.1345316390531508 1101 5 0.3935789675792236 1101 6 0.26029596358104357 1101 7 0.41384735536770023 1101 8 0.3107720611078264 1101 9 0.3351045656468088 1101 10-11 0.4629146180729222 1101 12-13 0.35247011725594035 1101 14-15 0.5081384535884155 1101 16-17 0.6139281449374323 1101 18-19 0.6023030836447347 1101 20-21 0.5999761246332653 1101 22-23 0.6119528124314897 1101 24-25 0.5864160512536287 1101 26-27 0.5315057936390204 1101 28-29 0.4809609024043837 1101 30-31 0.5710116810037036 1101 32-33 0.5760645002549509 1101 34-35 0.4270747034588709 1101 36-37 0.419180332268148 1101 38-39 0.48919125397956265 1101 40-41 0.381884071716712 1101 42-43 0.4132640922126143 1101 44-45 0.3869043955591991 1101 46-47 0.31434444462767175 1101 48-49 0.3556755973094212 1101 50-51 0.41516107015029746 1101 52-53 0.4479336198391124 1101 54-55 0.407887106424937 1101 56-57 0.4604941355837795 1101 58-59 0.5106115565537479 1101 60-61 0.39296420541496957 1101 62-63 0.3725024119000935 1101 64-65 0.45202865189070707 1101 66-67 0.5403471427259703 1101 68-69 0.5411297339030199 1101 70-71 0.5306609416421608 1101 72-73 0.5216060393020072 1101 74-75 0.4095229237094884 1101 76-77 0.3011856571692775 1101 78-79 0.3341220409402297 1101 80-81 0.37525636389999306 1101 82-83 0.4891380396620413 1101 84-85 0.4783385708603731 1101 86-87 0.5120104819662323 1101 88-89 0.4046261454774758 1101 90-91 0.44711266133996475 1101 92-93 0.44004854755323564 1101 94-95 0.6186722348398241 1101 96-97 0.764595552814658 1101 98-99 0.7234120923665301 1101 100-101 0.9466092432327464 1102 1 0.36568907025565167 1102 2 0.323617909488501 1102 3 0.28790547200591377 1102 4 0.2930856281967422 1102 5 0.39817669476320106 1102 6 0.3336131815292589 1102 7 0.2963027777131515 1102 8 0.2959149517236739 1102 9 0.30692328709016437 1102 10-11 0.45439866540372265 1102 12-13 0.48908940104553267 1102 14-15 0.5780761659326998 1102 16-17 0.5186927015040013 1102 18-19 0.5232286899242595 1102 20-21 0.5137631750491636 1102 22-23 0.4985731115684402 1102 24-25 0.5683085025364889 1102 26-27 0.640118243359808 1102 28-29 0.5915177032791448 1102 30-31 0.6512532555731596 1102 32-33 0.4031466262361221 1102 34-35 0.4447433487511674 1102 36-37 0.4817190912012492 1102 38-39 0.4497599656599576 1102 40-41 0.4836836187155811 1102 42-43 0.4317879333498027 1102 44-45 0.38407313848105673 1102 46-47 0.37343406855863037 1102 48-49 0.3570057414106458 1102 50-51 0.35813158602486794 1102 52-53 0.4454225095958151 1102 54-55 0.391302774015827 1102 56-57 0.4440455863589605 1102 58-59 0.44353423394664304 1102 60-61 0.45140279642523495 1102 62-63 0.5377189151999815 1102 64-65 0.40937586163804696 1102 66-67 0.4255821175375445 1102 68-69 0.43451908056604793 1102 70-71 0.5764918863057247 1102 72-73 0.5518313683136071 1102 74-75 0.4731569300358842 1102 76-77 0.4578540290988329 1102 78-79 0.4531221580956952 1102 80-81 0.4598875194433987 1102 82-83 0.4293294716923448 1102 84-85 0.4801533871215504 1102 86-87 0.37445711765179013 1102 88-89 0.3468791231788835 1102 90-91 0.384055684732008 1102 92-93 0.4366400264457262 1102 94-95 0.38855820352026527 1102 96-97 0.38256864610946195 1102 98-99 0.5069134006025564 1102 100-101 0.5445425427211639 1103 1 0.27074824345208626 1103 2 0.292397121071577 1103 3 0.27741675041156455 1103 4 0.3122172328888553 1103 5 0.225372997719532 1103 6 0.18592478417160407 1103 7 0.29463880991320934 1103 8 0.2806731438246004 1103 9 0.3288738875802437 1103 10-11 0.3210274050733446 1103 12-13 0.35406047130612706 1103 14-15 0.342501996789224 1103 16-17 0.37705780320065685 1103 18-19 0.26386520009392456 1103 20-21 0.416726586615475 1103 22-23 0.4525241220129601 1103 24-25 0.5170824592490177 1103 26-27 0.5153177886830633 1103 28-29 0.33676813885573864 1103 30-31 0.3446938727409332 1103 32-33 0.3599232290182286 1103 34-35 0.21120672770747007 1103 36-37 0.2841726685195809 1103 38-39 0.4344716838349214 1103 40-41 0.3334517894802431 1103 42-43 0.28672946825649603 1103 44-45 0.3330058593673044 1103 46-47 0.3303769637686784 1103 48-49 0.4534764809125491 1103 50-51 0.42135762931233245 1103 52-53 0.4772474963188813 1103 54-55 0.47413779530550926 1103 56-57 0.46074895637079294 1103 58-59 0.5164476869742529 1103 60-61 0.463659837858593 1103 62-63 0.4402398741206781 1103 64-65 0.4496922719279013 1103 66-67 0.4146925723074588 1103 68-69 0.3332322708501252 1103 70-71 0.34293249415635785 1103 72-73 0.3655810538599198 1103 74-75 0.3854427800857252 1103 76-77 0.349014815093291 1103 78-79 0.43625353917629894 1103 80-81 0.40783448624640783 1103 82-83 0.55216016982612 1103 84-85 0.4361160439990037 1103 86-87 0.5058725598901397 1103 88-89 0.4125642222667345 1103 90-91 0.5815829140921984 1103 92-93 0.528332896033632 1103 94-95 0.4691017658379941 1103 96-97 0.5764867483930978 1103 98-99 0.42330136657633943 1103 100-101 0.7162294493354082 1104 1 0.17817943428134697 1104 2 0.06889908795708521 1104 3 0.20428493002851056 1104 4 0.1526859383200545 1104 5 0.12360193289359955 1104 6 0.13946373162894332 1104 7 0.1796160022571982 1104 8 0.10498635392407607 1104 9 0.06394440501086507 1104 10-11 0.141342621691102 1104 12-13 0.18337131790294592 1104 14-15 0.26973569484061954 1104 16-17 0.3510539582417991 1104 18-19 0.14131031881003508 1104 20-21 0.24958850917011688 1104 22-23 0.3615547995253152 1104 24-25 0.29489751531060904 1104 26-27 0.27326832089062947 1104 28-29 0.2623663335678543 1104 30-31 0.2654023362790596 1104 32-33 0.3376841733084177 1104 34-35 0.2508971551456156 1104 36-37 0.2545209076377901 1104 38-39 0.15666541496956654 1104 40-41 0.22209689494684426 1104 42-43 0.2693213454446095 1104 44-45 0.24020268880225615 1104 46-47 0.30116485198917786 1104 48-49 0.21279275315102808 1104 50-51 0.20082308716936836 1104 52-53 0.16371133038884977 1104 54-55 0.27628579107260975 1104 56-57 0.3593589955002159 1104 58-59 0.21941206751380093 1104 60-61 0.2612519435861387 1104 62-63 0.35188667714341904 1104 64-65 0.2749597426097594 1104 66-67 0.31337005226396997 1104 68-69 0.21444753068787747 1104 70-71 0.23400355284933028 1104 72-73 0.36367918637460406 1104 74-75 0.25162835438794673 1104 76-77 0.2853826047467649 1104 78-79 0.31943951761721934 1104 80-81 0.30929034747263984 1104 82-83 0.36887994056925777 1104 84-85 0.21069292784369864 1104 86-87 0.23201976994448614 1104 88-89 0.20814948425102386 1104 90-91 0.3891496447113312 1104 92-93 0.3803788952340348 1104 94-95 0.10992034632466385 1104 96-97 0.2724776027660383 1104 98-99 0.43262819791482343 1104 100-101 0.6109091527780244 1105 1 0.16836205603764043 1105 2 0.16855074165062334 1105 3 0.19907185721773502 1105 4 0.12452547857171226 1105 5 0.11996398753071702 1105 6 0.18457735505071327 1105 7 0.24617461588569256 1105 8 0.23220788266736747 1105 9 0.22632122793017118 1105 10-11 0.22762239477126656 1105 12-13 0.22049104619451754 1105 14-15 0.2043847493976827 1105 16-17 0.21233512673688182 1105 18-19 0.22613174234749778 1105 20-21 0.2435000601611108 1105 22-23 0.3287367548652753 1105 24-25 0.33987230199536356 1105 26-27 0.2894147515038341 1105 28-29 0.39499713891677857 1105 30-31 0.38507889883367596 1105 32-33 0.3073334759392381 1105 34-35 0.0863251613204028 1105 36-37 0.2282818919493721 1105 38-39 0.3225837022908564 1105 40-41 0.29277155973716873 1105 42-43 0.3780251543917359 1105 44-45 0.3735412796079629 1105 46-47 0.34929116212874334 1105 48-49 0.3607407743681037 1105 50-51 0.2848500403837875 1105 52-53 0.29195073679343864 1105 54-55 0.40409353503342516 1105 56-57 0.5706157230915352 1105 58-59 0.5439559077858647 1105 60-61 0.35342135428690824 1105 62-63 0.27686071396883705 1105 64-65 0.3412843070993574 1105 66-67 0.28354482023043914 1105 68-69 0.30175976985805164 1105 70-71 0.3643311483492724 1105 72-73 0.446486631519285 1105 74-75 0.4081330880864016 1105 76-77 0.47524437973709865 1105 78-79 0.5194554732706465 1105 80-81 0.4926189025670027 1105 82-83 0.47509864239924937 1105 84-85 0.4323393652225498 1105 86-87 0.3896304756805158 1105 88-89 0.4141683316552154 1105 90-91 0.31082930424136634 1105 92-93 0.13550323938902054 1105 94-95 0.09270085573286124 1105 96-97 0.22155947612889193 1105 98-99 0.2862327519853203 1105 100-101 0.3036383644785339 1106 1 0.1446672024913056 1106 2 0.08834780489582883 1106 3 0.16936419826408766 1106 4 0.1497440918643207 1106 5 0.11130555045369306 1106 6 0.10318304748385998 1106 7 0.09885146786511712 1106 8 0.12988445218054778 1106 9 0.14488742927942866 1106 10-11 0.15898736513670286 1106 12-13 0.16103088592439008 1106 14-15 0.19273528607676838 1106 16-17 0.19648175963765624 1106 18-19 0.15688297520765815 1106 20-21 0.19381445118624185 1106 22-23 0.19928278040266179 1106 24-25 0.20324564934769285 1106 26-27 0.20838473733175533 1106 28-29 0.2500320082568379 1106 30-31 0.19981260903252718 1106 32-33 0.1837070442519746 1106 34-35 0.10604828208332862 1106 36-37 0.12235569597578433 1106 38-39 0.18872972338464677 1106 40-41 0.031819456122320844 1106 42-43 0.13605627255297748 1106 44-45 0.21787812947668783 1106 46-47 0.20403729948754545 1106 48-49 0.17497187077200493 1106 50-51 0.23519443921365735 1106 52-53 0.18289248466745534 1106 54-55 0.21505391619622571 1106 56-57 0.2661774671641197 1106 58-59 0.15076821458945489 1106 60-61 0.2623555922166929 1106 62-63 0.21325774933320218 1106 64-65 0.13330478475045737 1106 66-67 0.1611101996503379 1106 68-69 0.26177399748628716 1106 70-71 0.28921287492384806 1106 72-73 0.32471516310260284 1106 74-75 0.32764125000607436 1106 76-77 0.3101543759668104 1106 78-79 0.28916179508193807 1106 80-81 0.3219112413102785 1106 82-83 0.3857505392650715 1106 84-85 0.4199109521260347 1106 86-87 0.41283931117041206 1106 88-89 0.2860804574230045 1106 90-91 0.3391917261787398 1106 92-93 0.3148951119207446 1106 94-95 0.4126297772008769 1106 96-97 0.47043919604159967 1106 98-99 0.4429064809854424 1106 100-101 0.48015202791939515 1107 1 -0.06145988892250642 1107 2 0.07487869758593746 1107 3 0.11264975517657305 1107 4 0.043102165751157884 1107 5 0.018919001630848697 1107 6 0.08573675640037948 1107 7 0.05633850032224075 1107 8 0.14239431706938888 1107 9 0.14043340632090207 1107 10-11 0.11780726605068281 1107 12-13 0.07187524251246202 1107 14-15 0.12088773662748054 1107 16-17 -0.028130465987672437 1107 18-19 0.06822336695719855 1107 20-21 0.21287887267479988 1107 22-23 0.196031809632224 1107 24-25 0.2100200555191165 1107 26-27 0.17255019607767963 1107 28-29 0.21795556626168633 1107 30-31 0.14619981149227357 1107 32-33 0.17825382202995854 1107 34-35 0.04554367689204497 1107 36-37 0.15197908691304463 1107 38-39 0.2252396013490099 1107 40-41 0.28371281579587304 1107 42-43 -0.07726409375910492 1107 44-45 0.1519833844934908 1107 46-47 0.10164929914284926 1107 48-49 0.08539544599063476 1107 50-51 0.15421078730014415 1107 52-53 0.20229860844825964 1107 54-55 0.32507704665242443 1107 56-57 0.3032166950463022 1107 58-59 0.3611118830782871 1107 60-61 0.3269270667312867 1107 62-63 0.3513137171501839 1107 64-65 0.11512000497594244 1107 66-67 0.16844518397675046 1107 68-69 0.2323135636975593 1107 70-71 0.17935096285017949 1107 72-73 0.10041448956725674 1107 74-75 0.06234666444686354 1107 76-77 0.11017389187627913 1107 78-79 0.08100566808441556 1107 80-81 0.22768403481101984 1107 82-83 0.297160516429507 1107 84-85 0.1769715765669737 1107 86-87 -0.09132569410534686 1107 88-89 0.015610504446886608 1107 90-91 0.2961996938304594 1107 92-93 0.03109049302103628 1107 94-95 0.16615736825257343 1107 96-97 0.3271842831409053 1107 98-99 0.3748003038662553 1107 100-101 0.2833360283789865 1108 1 -0.06241801948158354 1108 2 0.04834852682732915 1108 3 -0.024944749388097875 1108 4 -0.010284142545053498 1108 5 0.06228665663897459 1108 6 -0.02474621190243198 1108 7 -0.07701991047176193 1108 8 -0.08542804658381442 1108 9 -0.02502221066170307 1108 10-11 -0.11410385626553676 1108 12-13 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-1.497688772423352 2316 100-101 -1.3177842018207642 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31810.0 3 7274.0 4 6086.0 5 4979.0 6 5020.0 7 5290.0 8 6108.0 9 7290.0 10 8653.0 11 10384.0 12 12923.0 13 18333.0 14 18960.0 15 14112.0 16 15848.0 17 18136.0 18 17195.0 19 15573.0 20 16398.0 21 17297.0 22 18522.0 23 20825.0 24 24509.0 25 28559.0 26 34240.0 27 41384.0 28 50338.0 29 61229.0 30 75676.0 31 94139.0 32 120174.0 33 157391.0 34 221926.0 35 335422.0 36 569105.0 37 947950.0 38 949114.0 39 169622.0 40 181.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.44561350070809 17.113048842222423 12.391517469512824 25.049820187556666 2 16.187433108420436 18.699552288718362 39.485104497484 25.627910105377204 3 20.537455314589597 21.898663758414646 27.35936812543656 30.204512801559197 4 13.142669206812435 14.553297462032457 35.18302956665525 37.12100376449986 5 15.220322040438413 36.18110059048088 33.24844432656743 15.350133042513283 6 35.96808113903332 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23.807721704028552 28-29 23.838029922321468 25.99684814000521 26.393212608589067 23.771909329084252 30-31 24.023407226005528 25.60677033305265 26.27323471800553 24.09658772293629 32-33 23.879662887175787 25.46448287571176 26.587342481815007 24.068511755297443 34-35 24.28478279859733 25.832402189053006 26.019482994137633 23.86333201821203 36-37 24.39386451874323 25.728681583336037 26.0378964682627 23.839557429658033 38-39 24.03095341381609 25.801705489288153 26.200508251390143 23.96683284550562 40-41 24.203153341503683 25.562445154690078 26.3514118867893 23.882989617016936 42-43 24.365934470014984 25.521202214362383 25.94776446222061 24.165098853402025 44-45 24.100899085881046 25.746611455608964 26.168836299598762 23.983653158911224 46-47 24.054943347355376 25.927268511213143 25.981655242486408 24.036132898945077 48-49 24.518220588870303 25.666740318254245 26.03358295889099 23.781456133984467 50-51 24.245312354333386 25.735627309990587 25.995338668302644 24.023721667373383 52-53 24.30817405436652 25.89527250315944 25.80167108953994 23.994882352934102 54-55 24.20454094462905 25.624803818636416 25.88973434788413 24.280920888850403 56-57 24.02166969398436 25.877397000646514 25.989321970629888 24.11161133473924 58-59 24.232023178651456 25.701276747523295 26.10702099900873 23.959679074816524 60-61 24.31546437646564 25.66685261582851 26.131865814050276 23.88581719365557 62-63 24.12316391749069 25.851830091052115 26.096803429819143 23.928202561638056 64-65 23.960720788374463 25.786973935049478 26.05884088344912 24.19346439312694 66-67 24.0289370745958 26.005636926933366 25.820653093330087 24.144772905140748 68-69 23.899706460102713 25.659839371485223 26.288111207205827 24.152342961206237 70-71 24.04491482603868 25.5119420067208 26.054925559210773 24.38821760802975 72-73 23.98643119276757 25.78681873210204 26.0085480888019 24.218201986328495 74-75 23.84991407032438 25.999905945823592 25.805146447283896 24.34503353656813 76-77 24.229346026141638 25.826092812552986 25.758676298513492 24.18588486279188 78-79 24.04834376742598 25.870945793105886 25.905603840717774 24.17510659875036 80-81 24.011212602909872 26.06494833731516 25.87128091565341 24.052558144121562 82-83 24.321184867197797 26.015358988731684 25.76641911781242 23.897037026258104 84-85 24.343927219864835 25.799869059639434 25.473961292259172 24.38224242823655 86-87 24.079833325395988 25.64929879578465 26.066201778830962 24.204666099988405 88-89 24.164896666851497 25.827303266008993 25.815383656856707 24.19241641028281 90-91 24.088848593650876 26.027736101000205 25.848500893228486 24.03491441212043 92-93 24.048955358465005 25.79679907826773 25.942245216342556 24.212000346924707 94-95 24.157044002111274 25.798935005870465 25.99671378875047 24.04730720326779 96-97 24.12537031134827 25.703109154578634 25.992781866733477 24.178738667339612 98-99 23.839123884048654 25.704144968155347 25.85806970636368 24.598661441432323 100-101 24.034016962795224 25.690333106305115 25.76605192714498 24.509598003754682 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 67.0 1 60.0 2 105.0 3 161.0 4 138.5 5 150.0 6 277.5 7 520.0 8 785.5 9 917.5 10 1002.5 11 1142.0 12 1364.0 13 1674.5 14 2043.0 15 2374.0 16 2655.0 17 2872.0 18 2914.0 19 2910.0 20 2943.0 21 2982.0 22 3067.0 23 3445.5 24 4184.0 25 5145.0 26 7004.0 27 9714.0 28 12851.0 29 17175.5 30 22616.0 31 29694.0 32 38434.0 33 45746.5 34 52813.5 35 60940.0 36 70399.0 37 80364.5 38 87992.5 39 94891.0 40 103826.0 41 115503.5 42 126772.5 43 135492.5 44 142010.0 45 149004.5 46 154966.5 47 160744.0 48 169329.0 49 172257.0 50 166868.0 51 163651.5 52 168415.0 53 171241.0 54 172232.5 55 167010.5 56 152980.5 57 138944.5 58 129269.5 59 118670.5 60 104646.0 61 91011.0 62 76414.0 63 62281.5 64 47715.0 65 34961.5 66 25403.5 67 20845.0 68 15859.0 69 10999.0 70 8944.5 71 6345.5 72 4767.5 73 3885.0 74 2814.5 75 2003.0 76 1284.5 77 604.0 78 301.0 79 184.0 80 124.5 81 89.5 82 77.0 83 66.0 84 58.5 85 58.5 86 54.5 87 45.0 88 41.0 89 39.0 90 34.5 91 31.0 92 32.5 93 31.0 94 32.5 95 43.0 96 41.0 97 37.5 98 31.0 99 23.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04684087386832137 2 0.012111130392115798 3 0.010459612611372734 4 0.006270980558763516 5 0.00868841962912655 6 0.006151305357260396 7 0.0053135789467385515 8 0.009526146039648393 9 0.015174815550595683 10-11 0.002477276671114595 12-13 0.008999575153034664 14-15 0.009119250354537783 16-17 0.01923180488155147 18-19 0.012218838073468607 20-21 0.0037099312465967366 22-23 0.010244197248667118 24-25 0.022044172116874802 26-27 0.007707082976800963 28-29 0.018166695588173697 30-31 0.015138912990144747 32-33 0.013415590088499811 34-35 0.264697610684602 36-37 0.46769068747419507 38-39 0.46441158695300955 40-41 0.48488801393019343 42-43 0.4750626798867873 44-45 0.4900579826351283 46-47 0.4957186196662258 48-49 0.5030068394377659 50-51 0.5131074264446293 52-53 0.5023127232690477 54-55 0.48995027495377547 56-57 0.4853786822563562 58-59 0.4830928859076466 60-61 0.47938295466104996 62-63 0.47801865736391436 64-65 0.4933131481160131 66-67 0.46681705850322225 68-69 0.49350462843841814 70-71 0.47482332948378103 72-73 0.4835715867136592 74-75 0.4977650656119292 76-77 0.4844452156846319 78-79 0.4837391319957635 80-81 0.4866472393922894 82-83 0.4869464273960471 84-85 0.48724561539980493 86-87 0.4885859776566399 88-89 0.5016664771809309 90-91 0.52617595844877 92-93 0.5134903870894393 94-95 0.48586935058251907 96-97 0.45820044399499754 98-99 0.4499667901315829 100-101 0.4587748849622125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4177975.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.777778052616306 #Duplication Level Percentage of deduplicated Percentage of total 1 65.89716819691395 26.21242930832769 2 15.205606625801446 12.096904910330435 3 6.723114716799203 8.02291695081351 4 3.5537548796668483 5.654418914271602 5 2.1683486627581883 4.312604592394129 6 1.430506337751257 3.4141418163558286 7 0.9724420807464632 2.707710967987004 8 0.7111002445429063 2.2628790160471124 9 0.5224242378732744 1.87027878450873 >10 2.5498159351185254 17.618314995801963 >50 0.15521452666699648 4.284126210185277 >100 0.10078903365209095 7.822268885157779 >500 0.007240637372432582 1.9425270408035156 >1k 0.00247388433628222 1.7784776070153538 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.787008060124821E-5 2.3935040300624105E-5 3 0.0 0.0 0.0 4.787008060124821E-5 2.3935040300624105E-5 4 0.0 0.0 0.0 9.574016120249642E-5 4.787008060124821E-5 5 0.0 0.0 0.0 9.574016120249642E-5 4.787008060124821E-5 6 0.0 0.0 0.0 9.574016120249642E-5 7.180512090187232E-5 7 0.0 0.0 0.0 9.574016120249642E-5 7.180512090187232E-5 8 0.0 0.0 0.0 9.574016120249642E-5 7.180512090187232E-5 9 0.0 0.0 0.0 1.4361024180374463E-4 1.1967520150312053E-4 10-11 0.0 0.0 0.0 1.795128022546808E-4 1.1967520150312053E-4 12-13 0.0 0.0 0.0 1.9148032240499284E-4 1.1967520150312053E-4 14-15 0.0 2.3935040300624105E-5 0.0 2.1541536270561695E-4 1.3164272165343258E-4 16-17 0.0 2.3935040300624105E-5 0.0 3.350905642087375E-4 1.4361024180374463E-4 18-19 0.0 4.787008060124821E-5 0.0 3.590256045093616E-4 1.5557776195405668E-4 20-21 0.0 4.787008060124821E-5 0.0 4.1886320526092187E-4 1.6754528210436874E-4 22-23 0.0 4.787008060124821E-5 0.0 5.864084873652906E-4 2.3935040300624105E-4 24-25 0.0 4.787008060124821E-5 0.0 7.659212896199714E-4 3.1115552390811337E-4 26-27 0.0 4.787008060124821E-5 0.0 8.855964911230918E-4 3.1115552390811337E-4 28-29 0.0 4.787008060124821E-5 0.0 0.0014839724986386946 3.1115552390811337E-4 30-31 0.0 4.787008060124821E-5 0.0 0.0034346782831395587 3.1115552390811337E-4 32-33 0.0 4.787008060124821E-5 0.0 0.006977064247631927 3.829606448099857E-4 34-35 0.0 7.180512090187232E-5 0.0 0.011811942388357997 3.829606448099857E-4 36-37 0.0 7.180512090187232E-5 0.0 0.01998575865102113 3.829606448099857E-4 38-39 0.0 7.180512090187232E-5 0.0 0.03338938121937063 3.829606448099857E-4 40-41 0.0 7.180512090187232E-5 0.0 0.049377988140187526 3.829606448099857E-4 42-43 0.0 7.180512090187232E-5 0.0 0.06640776931408159 3.829606448099857E-4 44-45 0.0 7.180512090187232E-5 0.0 0.0856874442762343 3.829606448099857E-4 46-47 0.0 8.377264105218437E-5 0.0 0.10900017352904218 4.427982455615459E-4 48-49 0.0 9.574016120249642E-5 0.0 0.13568774346423806 4.54765765711858E-4 50-51 0.0 9.574016120249642E-5 0.0 0.16496029775190135 4.54765765711858E-4 52-53 0.0 9.574016120249642E-5 0.0 0.1949150006881324 5.026358463131063E-4 54-55 0.0 9.574016120249642E-5 0.0 0.22921391343892678 5.505059269143545E-4 56-57 0.0 9.574016120249642E-5 0.0 0.2679168736050359 5.505059269143545E-4 58-59 0.0 9.574016120249642E-5 0.0 0.31184964007683147 5.505059269143545E-4 60-61 0.0 9.574016120249642E-5 0.0 0.3612036931767184 5.744409672149785E-4 62-63 0.0 9.574016120249642E-5 0.0 0.4109885770020165 5.744409672149785E-4 64-65 0.0 9.574016120249642E-5 0.0 0.4657998192904457 5.744409672149785E-4 66-67 0.0 9.574016120249642E-5 0.0 0.5294789940102562 5.744409672149785E-4 68-69 0.0 9.574016120249642E-5 0.0 0.6011405046703248 5.744409672149785E-4 70-71 0.0 9.574016120249642E-5 0.0 0.6807245136698998 5.744409672149785E-4 72-73 0.0 9.574016120249642E-5 0.0 0.7654904588945601 5.983760075156027E-4 74-75 0.0 9.574016120249642E-5 0.0 0.8529730311933412 5.983760075156027E-4 76-77 0.0 9.574016120249642E-5 0.0 0.9508673460228938 5.983760075156027E-4 78-79 0.0 9.574016120249642E-5 0.0 1.0625243090253054 5.983760075156027E-4 80-81 0.0 9.574016120249642E-5 0.0 1.1925873180188966 6.342785679665389E-4 82-83 0.0 9.574016120249642E-5 0.0 1.3258815574530725 6.70181128417475E-4 84-85 0.0 9.574016120249642E-5 0.0 1.464656921116091 6.70181128417475E-4 86-87 0.0 9.574016120249642E-5 0.0 1.6141791178740896 6.70181128417475E-4 88-89 0.0 9.574016120249642E-5 0.0 1.7849915329794936 6.70181128417475E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7230 0.0 34.903027 1 ATCAACG 8130 0.0 30.041674 3 TCAACGC 8165 0.0 29.97015 4 GGTATCA 4620 0.0 29.513767 1 CAACGCA 8420 0.0 29.006289 5 AACGCAG 8715 0.0 28.132385 6 TATCAAC 8825 0.0 28.050898 2 TACATGG 12035 0.0 26.861755 2 GTACATG 12680 0.0 26.36086 1 ACATGGG 12375 0.0 24.90008 3 GTGTAGC 2535 0.0 24.466255 1 ACGCAGA 10215 0.0 23.72362 7 CGCAGAG 10615 0.0 22.875065 8 CATGGGG 8310 0.0 22.840096 4 GAGTACA 9565 0.0 19.898268 1 AGTACAT 8690 0.0 19.281609 2 GTAGCCC 2335 0.0 18.852156 3 GTACCTG 3335 0.0 18.171396 1 TATAGCG 235 2.6177804E-6 18.127136 5 GCAGAGT 13235 0.0 18.024414 9 >>END_MODULE