FastQCFastQC Report
Thu 26 May 2016
SRR936344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936344_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177975
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG45490.10888049832753907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80200.032.6238441
GGTATCA55050.032.492061
ATCAACG89400.029.0226443
TCAACGC89800.028.8405464
CAACGCA92800.027.9082015
TATCAAC96850.026.9441552
AACGCAG97050.026.8815526
TACATGG119850.025.455082
GTACATG126900.024.516751
ACATGGG122650.023.977913
ACGCAGA112750.023.1804687
CGCAGAG116600.022.3743958
AGTACAT87050.021.3112772
GGACCGT5800.021.2633916
GAGTACA97450.021.0886461
TACCTGG33400.020.455852
GTGTAGC26100.020.4138221
CATGGGG87050.020.2703534
GTACCTG35750.020.0931341
GTATAGG12600.018.5000251
Produced by FastQC (version 0.11.5)