##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936344_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4177975 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.508283079721636 34.0 31.0 34.0 31.0 34.0 2 32.649912696940504 34.0 31.0 34.0 31.0 34.0 3 32.733885434929604 34.0 31.0 34.0 31.0 34.0 4 36.21053716214195 37.0 35.0 37.0 35.0 37.0 5 36.16702637043065 37.0 35.0 37.0 35.0 37.0 6 36.194955211555836 37.0 37.0 37.0 35.0 37.0 7 36.14666818255255 37.0 37.0 37.0 35.0 37.0 8 36.12164290116623 37.0 36.0 37.0 35.0 37.0 9 37.902541781604725 39.0 38.0 39.0 35.0 39.0 10-11 37.838418372536935 39.0 38.0 39.0 35.0 39.0 12-13 37.784106893889984 39.0 38.0 39.0 35.0 39.0 14-15 39.173676960728585 41.0 39.0 41.0 36.0 41.0 16-17 39.09827727547436 40.0 38.0 41.0 36.0 41.0 18-19 39.091837911907085 40.0 38.5 41.0 36.0 41.0 20-21 39.03463137046057 40.0 39.0 41.0 35.0 41.0 22-23 38.921990916652206 40.0 38.0 41.0 35.0 41.0 24-25 39.07292480208713 40.0 39.0 41.0 35.5 41.0 26-27 39.00669869972893 40.0 39.0 41.0 35.0 41.0 28-29 38.92356285042395 40.0 38.0 41.0 35.0 41.0 30-31 38.83592051173116 40.0 38.0 41.0 35.0 41.0 32-33 38.7125241295125 40.0 38.0 41.0 35.0 41.0 34-35 38.59769134568781 40.0 38.0 41.0 34.0 41.0 36-37 38.47050006282948 40.0 38.0 41.0 34.0 41.0 38-39 38.35539621467338 40.0 38.0 41.0 34.0 41.0 40-41 38.22881599339393 40.0 38.0 41.0 33.5 41.0 42-43 38.28375947678002 40.0 38.0 41.0 34.0 41.0 44-45 38.19744780665275 40.0 38.0 41.0 33.0 41.0 46-47 38.0590205781509 40.0 38.0 41.0 33.0 41.0 48-49 37.880612018980486 40.0 37.0 41.0 33.0 41.0 50-51 37.69638377443618 40.0 37.0 41.0 33.0 41.0 52-53 37.487635158180694 40.0 36.5 41.0 32.5 41.0 54-55 37.293100245932536 40.0 36.0 41.0 32.0 41.0 56-57 37.04017317001657 39.0 35.5 41.0 31.5 41.0 58-59 36.779291881832705 39.0 35.0 41.0 31.0 41.0 60-61 36.49912649070423 38.5 35.0 40.5 31.0 41.0 62-63 36.20189756999503 38.0 35.0 40.0 31.0 41.0 64-65 35.82398638096207 37.0 35.0 40.0 30.0 41.0 66-67 35.47723765699891 37.0 35.0 40.0 30.0 41.0 68-69 35.11948802948797 36.0 34.0 39.0 29.5 41.0 70-71 34.74516374559445 36.0 34.0 39.0 29.0 40.5 72-73 34.33752320202969 35.0 34.0 38.5 29.0 40.0 74-75 33.9131769577367 35.0 34.0 37.0 29.0 39.5 76-77 33.18410126915551 35.0 33.0 36.0 27.5 39.0 78-79 33.23081839886548 35.0 33.0 36.0 28.0 39.0 80-81 33.07315505717483 35.0 33.0 36.0 28.0 37.5 82-83 32.804206583332835 35.0 33.0 35.5 27.5 37.0 84-85 32.52854205685769 35.0 33.0 35.0 27.0 37.0 86-87 32.135334222919 35.0 33.0 35.0 26.0 36.0 88-89 31.663203705144237 35.0 32.0 35.0 25.0 36.0 90-91 31.463141354364254 35.0 32.0 35.0 25.0 35.5 92-93 31.194128614939054 34.0 32.0 35.0 24.0 35.0 94-95 31.047850693218606 34.0 32.0 35.0 24.0 35.0 96-97 30.814345227053778 34.0 32.0 35.0 23.0 35.0 98-99 30.39355836260389 34.0 31.5 35.0 18.5 35.0 100-101 27.041535432835282 31.5 25.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16628560860633002 1101 2 0.14380841730736904 1101 3 0.21414071743296148 1101 4 0.19304710207376985 1101 5 0.14446563342932706 1101 6 0.13030220843607765 1101 7 0.14976116456881527 1101 8 0.13430347500851525 1101 9 0.2449334275424846 1101 10-11 0.187689798979207 1101 12-13 0.262367633596277 1101 14-15 0.36591179597521517 1101 16-17 0.35215968957555077 1101 18-19 0.35128477684283865 1101 20-21 0.23344907744946397 1101 22-23 0.32572552206850247 1101 24-25 0.24274545751006826 1101 26-27 0.26537258494455784 1101 28-29 0.313445708933223 1101 30-31 0.3424906020763885 1101 32-33 0.36571611460458087 1101 34-35 0.36275763353436474 1101 36-37 0.3103580198745064 1101 38-39 0.3562571547938518 1101 40-41 0.3851151852213306 1101 42-43 0.337339405052802 1101 44-45 0.3611452805977038 1101 46-47 0.3513319938428836 1101 48-49 0.38070334030571473 1101 50-51 0.3713763848740399 1101 52-53 0.34357369163041085 1101 54-55 0.38483690612149957 1101 56-57 0.33235640920877785 1101 58-59 0.18759114173843017 1101 60-61 0.33802114763082614 1101 62-63 0.36437987479772715 1101 64-65 0.4263783737868394 1101 66-67 0.42159720849603133 1101 68-69 0.5110894862441029 1101 70-71 0.4665357937322625 1101 72-73 0.45032664530267397 1101 74-75 0.3769108122439562 1101 76-77 0.2266775693577685 1101 78-79 0.20199356242201105 1101 80-81 0.313546255784388 1101 82-83 0.3336967759345626 1101 84-85 0.528729080556154 1101 86-87 0.3729006455483912 1101 88-89 0.419443105498047 1101 90-91 0.5642489830246511 1101 92-93 0.6059096596028795 1101 94-95 0.483696930720221 1101 96-97 0.5854155139129986 1101 98-99 0.584363336778285 1101 100-101 0.8795007653686895 1102 1 0.04758939272785767 1102 2 0.1627947101179288 1102 3 0.14754947285140219 1102 4 0.09852927491713359 1102 5 0.07837011267328364 1102 6 0.13851496276107156 1102 7 0.15015582227960067 1102 8 0.16351900201285474 1102 9 0.1991087702222245 1102 10-11 0.22172537518883928 1102 12-13 0.2601197716835131 1102 14-15 0.2982010918708653 1102 16-17 0.32728867774284254 1102 18-19 0.38196009999666103 1102 20-21 0.2542685799292599 1102 22-23 0.31202270643214547 1102 24-25 0.254656927030112 1102 26-27 0.26075466796873314 1102 28-29 0.23176120860127725 1102 30-31 0.21266176621119115 1102 32-33 0.26162004712303855 1102 34-35 0.2685289460660911 1102 36-37 0.22948224371954495 1102 38-39 0.20978888439903187 1102 40-41 0.1426337934144044 1102 42-43 0.19773988148502752 1102 44-45 0.2904759714244278 1102 46-47 0.25386993268773494 1102 48-49 0.3606554627722218 1102 50-51 0.28182489504703767 1102 52-53 0.29970252359041893 1102 54-55 0.10788642383150204 1102 56-57 0.33255014529656535 1102 58-59 0.23957290453765978 1102 60-61 0.28318805511705847 1102 62-63 0.27639647182197535 1102 64-65 0.2577280047471291 1102 66-67 0.2962914717834124 1102 68-69 0.22951542446412532 1102 70-71 0.20319651150474272 1102 72-73 0.12386833312240952 1102 74-75 0.22518554181008454 1102 76-77 0.1335530330981527 1102 78-79 0.1846181963111846 1102 80-81 0.20321326090017777 1102 82-83 0.32021682904504445 1102 84-85 0.3318382694498183 1102 86-87 0.35636629947115495 1102 88-89 0.3730020542230079 1102 90-91 0.46478879635693815 1102 92-93 0.43005340935880554 1102 94-95 0.42324556976423366 1102 96-97 0.2919896928484462 1102 98-99 0.2414480011278819 1102 100-101 0.7429601709999609 1103 1 0.0023796917150846753 1103 2 0.06371520690817079 1103 3 0.0959934535090099 1103 4 -0.023433139714512663 1103 5 0.06459541990618334 1103 6 0.09691494785363375 1103 7 0.11389691741698016 1103 8 0.12500393152863865 1103 9 0.11732382859414514 1103 10-11 0.14043301245369832 1103 12-13 0.20709022701245772 1103 14-15 0.2900858201362908 1103 16-17 0.2607063374620253 1103 18-19 0.17705540508661244 1103 20-21 0.21160536681816922 1103 22-23 0.24505512078427927 1103 24-25 0.2665947630297083 1103 26-27 0.19762701374666847 1103 28-29 0.2210049358325037 1103 30-31 0.20964248903541005 1103 32-33 0.2181307385484601 1103 34-35 0.23829301728635954 1103 36-37 0.283763863784948 1103 38-39 0.31569815402792756 1103 40-41 0.3228778281114515 1103 42-43 0.3761790731294141 1103 44-45 0.3284040989575274 1103 46-47 0.3626913323094527 1103 48-49 0.3825167677317438 1103 50-51 0.3943708052856465 1103 52-53 0.3560940673483941 1103 54-55 0.32978555640517726 1103 56-57 0.3237948974138902 1103 58-59 0.2768835661482214 1103 60-61 0.2657183311841109 1103 62-63 0.26398465395354975 1103 64-65 0.2193493745539783 1103 66-67 0.23323943552107806 1103 68-69 0.16709263099730265 1103 70-71 0.10147831791307738 1103 72-73 0.18244053634800395 1103 74-75 0.27817204126503015 1103 76-77 0.19561529027002678 1103 78-79 0.0753143921203403 1103 80-81 0.23599455952403048 1103 82-83 0.16410834019300324 1103 84-85 0.24335217529073816 1103 86-87 0.1460777474488637 1103 88-89 0.28339022517400636 1103 90-91 0.42890980745366036 1103 92-93 0.5333560768578067 1103 94-95 0.45025053580323515 1103 96-97 0.35706399709054537 1103 98-99 0.2555623225135264 1103 100-101 0.5754745892839317 1104 1 -0.06315261421299567 1104 2 -0.005038709360775329 1104 3 -0.015324484174364272 1104 4 -0.011377540411821485 1104 5 -0.05122879451123907 1104 6 -0.018973850648073665 1104 7 -0.012675086677056413 1104 8 -0.0033404825917386916 1104 9 -0.08064143283471026 1104 10-11 -0.052175422271105276 1104 12-13 0.07350687203055628 1104 14-15 0.09068481039704324 1104 16-17 0.09114476575879848 1104 18-19 0.12152781025287851 1104 20-21 0.03281192335390415 1104 22-23 0.05085638434693607 1104 24-25 0.06357053553635694 1104 26-27 0.1403612174777038 1104 28-29 0.1599200360398143 1104 30-31 -0.03814599425546561 1104 32-33 0.007633633477603041 1104 34-35 -0.02995469862120359 1104 36-37 -0.0726968155730745 1104 38-39 -0.0019958383359579557 1104 40-41 0.08203820897963965 1104 42-43 0.09828536623729889 1104 44-45 0.10990637827668337 1104 46-47 0.07183781277338142 1104 48-49 -0.1245705580503369 1104 50-51 0.022418190476415134 1104 52-53 0.01947129712656448 1104 54-55 -0.05473788392896495 1104 56-57 -0.0379356407183451 1104 58-59 -0.038517474255804984 1104 60-61 -0.15137027760095378 1104 62-63 -0.11375275656168782 1104 64-65 -0.10668109958721317 1104 66-67 -0.20159703150241626 1104 68-69 -0.0800119697616779 1104 70-71 -0.0769826585461999 1104 72-73 -0.1273005733237298 1104 74-75 -0.025367622186074357 1104 76-77 -0.03129799695427238 1104 78-79 -0.017782010437684903 1104 80-81 5.79280245965208E-4 1104 82-83 0.007413646589164102 1104 84-85 0.01016873537317764 1104 86-87 0.07664005713193234 1104 88-89 0.1413406055041122 1104 90-91 0.2339886043758419 1104 92-93 0.23847281421740263 1104 94-95 0.2360130142004735 1104 96-97 0.09639012111396994 1104 98-99 0.08398229928929268 1104 100-101 0.27936126381544213 1105 1 -0.05427707056497866 1105 2 -0.001235287521488715 1105 3 0.07260723464980856 1105 4 0.0407348640656906 1105 5 -0.03313096775017499 1105 6 -0.018771304058127214 1105 7 0.01875148969075724 1105 8 0.018022896984795977 1105 9 0.03769524949443337 1105 10-11 0.06072372277649407 1105 12-13 0.09201056720203127 1105 14-15 0.08026613517859715 1105 16-17 0.07593798500074911 1105 18-19 0.0701964857532289 1105 20-21 0.08531849730863428 1105 22-23 -0.0036213893714531764 1105 24-25 0.08688848265830984 1105 26-27 0.06824187539187676 1105 28-29 -0.05340775510011753 1105 30-31 0.009250823972998035 1105 32-33 0.04211729612885762 1105 34-35 0.06899093150307323 1105 36-37 0.05881089219151647 1105 38-39 0.07372296948919654 1105 40-41 0.13657412498637456 1105 42-43 0.15672555452528059 1105 44-45 0.14688882029225425 1105 46-47 0.19939494585256057 1105 48-49 0.21570799495795256 1105 50-51 0.048191222152041746 1105 52-53 0.09128252032191142 1105 54-55 0.1612854541741484 1105 56-57 0.19738811809531853 1105 58-59 0.14819693533427625 1105 60-61 0.15203387233483312 1105 62-63 0.22116500698567165 1105 64-65 0.2077768507822455 1105 66-67 0.20620071621333125 1105 68-69 0.13747380546319476 1105 70-71 0.10656874008240891 1105 72-73 0.029809826830529573 1105 74-75 0.054043960941754676 1105 76-77 0.08624900738589503 1105 78-79 0.03276273699503207 1105 80-81 0.03176215233123969 1105 82-83 0.08587240094313842 1105 84-85 0.13343482662147466 1105 86-87 0.08548071540693769 1105 88-89 0.21910341428547042 1105 90-91 0.2639027764916726 1105 92-93 0.3135461369286716 1105 94-95 0.1638519506180387 1105 96-97 0.09144203618704694 1105 98-99 0.10670782934963086 1105 100-101 0.33945487783296 1106 1 -0.011785748104088611 1106 2 0.056138144909404275 1106 3 0.015002681158492237 1106 4 -0.019571272442320264 1106 5 -0.021933179467630737 1106 6 0.004749395840384807 1106 7 0.03170808756707544 1106 8 0.03792965467468434 1106 9 0.032555786359125705 1106 10-11 0.10121193959894015 1106 12-13 0.0478374867904634 1106 14-15 0.14105083859513456 1106 16-17 0.05712915395444895 1106 18-19 0.11474584913393215 1106 20-21 0.09416372111606108 1106 22-23 0.10231246096316937 1106 24-25 0.12237613251613055 1106 26-27 0.05230177015727833 1106 28-29 0.11449126369208074 1106 30-31 0.13167765471664694 1106 32-33 0.1151604248631557 1106 34-35 0.19468417614336175 1106 36-37 0.11656477346058836 1106 38-39 0.2049826957773675 1106 40-41 0.10846724355324255 1106 42-43 0.09451693674438388 1106 44-45 0.043798327997620845 1106 46-47 0.07925609167924108 1106 48-49 0.0954870765511373 1106 50-51 0.10069309408360994 1106 52-53 0.005079208029265203 1106 54-55 -0.01936583169754158 1106 56-57 0.10588829279086553 1106 58-59 0.14960272986169088 1106 60-61 0.10194380150422688 1106 62-63 0.11291540762034202 1106 64-65 0.1529208225394143 1106 66-67 0.05587075906284866 1106 68-69 0.1211887120806665 1106 70-71 0.0934234926887143 1106 72-73 -0.15174931367549505 1106 74-75 0.03861896710927226 1106 76-77 -0.030105137816384797 1106 78-79 -0.11208182054429017 1106 80-81 -0.08185715063962107 1106 82-83 -0.12253590109876455 1106 84-85 0.00399578832351466 1106 86-87 -0.04409075011056274 1106 88-89 0.08223463319978919 1106 90-91 0.2123126202438108 1106 92-93 0.16058456981408042 1106 94-95 0.18975366482912648 1106 96-97 0.24621660306197768 1106 98-99 0.14571431767374676 1106 100-101 0.4560070203563491 1107 1 -0.009494889137691587 1107 2 0.029510821648536023 1107 3 0.020218283620387467 1107 4 0.0339871712730897 1107 5 -0.025294214125203496 1107 6 -0.008349632516313932 1107 7 0.02780991332004845 1107 8 -0.04462500708559958 1107 9 -0.06161877030597651 1107 10-11 0.009452980972319835 1107 12-13 0.062330136087688004 1107 14-15 0.0425859116264391 1107 16-17 0.08938948784063427 1107 18-19 0.0859837037665514 1107 20-21 0.018599107285204752 1107 22-23 -0.01301421873868236 1107 24-25 0.05592776444190406 1107 26-27 0.12476477321619228 1107 28-29 0.16878948278534978 1107 30-31 0.005305220639698405 1107 32-33 0.061855562779641104 1107 34-35 0.058853376713706496 1107 36-37 0.04669573105535818 1107 38-39 0.1213005138235772 1107 40-41 0.07137397482410535 1107 42-43 -0.05869195936664795 1107 44-45 -0.06923530189268234 1107 46-47 -0.016080864825113395 1107 48-49 -0.10772417384904287 1107 50-51 -0.07798288151885657 1107 52-53 0.08366325855140389 1107 54-55 0.0665573414140681 1107 56-57 -0.08556013614016678 1107 58-59 -0.003005387824671857 1107 60-61 0.06245093138371516 1107 62-63 0.018921926045386783 1107 64-65 0.09733647301907666 1107 66-67 -0.15227326612338032 1107 68-69 0.05466738538552818 1107 70-71 0.18292087316820016 1107 72-73 0.08798169087986452 1107 74-75 0.03241873624288161 1107 76-77 -0.16087491108060448 1107 78-79 -0.0665608921656613 1107 80-81 0.08303833943958239 1107 82-83 0.13518795824118968 1107 84-85 -0.037767458100027795 1107 86-87 0.033250639134244864 1107 88-89 0.04980890789268244 1107 90-91 -0.2629339246021267 1107 92-93 0.021610568330011404 1107 94-95 0.11126921082286145 1107 96-97 -0.034271458814163935 1107 98-99 -0.08062857129150558 1107 100-101 0.27008362468269453 1108 1 -0.09532521243856706 1108 2 -0.04934465698583068 1108 3 -0.02852889550036508 1108 4 -0.010597765925361102 1108 5 -0.06603362794525225 1108 6 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-0.6000224034619634 2316 74-75 -0.5753506752884618 2316 76-77 -0.42980686794245315 2316 78-79 -0.5795093031787175 2316 80-81 -0.6141724001893962 2316 82-83 -0.8712214233524307 2316 84-85 -0.6553977028007409 2316 86-87 -0.797604063720037 2316 88-89 -0.7050973387580193 2316 90-91 -0.7626721044796234 2316 92-93 -0.7666247175540697 2316 94-95 -0.7510707177281191 2316 96-97 -0.6598927036012583 2316 98-99 -0.7538507389251698 2316 100-101 -0.9117211440377844 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 30.0 5 60.0 6 109.0 7 255.0 8 613.0 9 1111.0 10 1746.0 11 2647.0 12 3876.0 13 5432.0 14 6894.0 15 8644.0 16 10650.0 17 13140.0 18 12870.0 19 10561.0 20 10812.0 21 11866.0 22 13221.0 23 15183.0 24 18024.0 25 22915.0 26 28425.0 27 36450.0 28 45721.0 29 57458.0 30 72776.0 31 90439.0 32 114599.0 33 149648.0 34 206798.0 35 313395.0 36 547944.0 37 1012523.0 38 1128014.0 39 202920.0 40 193.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.74647745699669 17.48623152634291 12.773356608567376 24.993934408093025 2 16.402840806258084 18.940448021174376 39.02622953017435 25.63048164239319 3 20.403544779468522 22.041587132522334 27.74044842298003 29.814419665029114 4 13.313794362101259 14.940180350528664 35.032665346250276 36.7133599411198 5 15.120913839838678 36.167712827386474 33.377030738575506 15.334342594199343 6 35.85729928972768 33.74342833549746 16.248493588401082 14.150778786373783 7 31.581304340021184 30.172009167120432 19.14975556340093 19.096930929457454 8 27.372112087793727 35.106050179811994 18.636013858388335 18.885823874005947 9 26.621030523160144 15.872186884794667 18.83989253166905 38.66689006037614 10-11 26.58529930241158 25.21113747961029 26.411674104498246 21.791889113479886 12-13 26.610915555113024 22.004364077464828 27.03865881389626 24.346061553525892 14-15 23.19341307690927 23.970655640591435 24.623088936625997 28.2128423458733 16-17 24.195233115578958 27.035945149162906 25.08780025797347 23.681021477284666 18-19 23.909358002381538 25.97393474111262 26.45597448524704 23.660732771258804 20-21 24.347814639756486 25.572008911020838 26.414403158469806 23.66577329075287 22-23 23.894030468591897 25.381820784210145 26.428715637206494 24.295433109991457 24-25 23.873036396856822 25.55903725686351 26.697295478837386 23.870630867442287 26-27 23.915402437291977 25.86543707811233 26.47680058364606 23.742359900949634 28-29 23.698443373939234 26.027578088610447 26.596789952420867 23.677188585029448 30-31 23.89849810863365 25.626506127912087 26.464851510268716 24.010144253185548 32-33 23.750340630964033 25.478825110698335 26.765054811516336 24.0057794468213 34-35 24.117928440954817 25.83165755384904 26.26809623432214 23.782317770874002 36-37 24.237785193655533 25.738213930321464 26.24709712665653 23.77690374936647 38-39 23.87759744957388 25.83528334404627 26.41617570836161 23.870943498018235 40-41 24.062042875338726 25.605144341739887 26.484475219243315 23.84833756367807 42-43 24.232508959522526 25.561121327104402 26.09338488337511 24.11298482999796 44-45 23.93731455604034 25.756388750858108 26.32827901303362 23.97801768006794 46-47 23.926246725097545 25.973769181870747 26.158057813032435 23.94192627999927 48-49 24.394292722799054 25.722437462723242 26.193547445000366 23.68972236947734 50-51 24.12550432512917 25.79581072190647 26.109916218986047 23.968768733978315 52-53 24.235666460930247 25.924045560743046 25.891048742297002 23.9492392360297 54-55 24.12355798257881 25.611222708703956 26.01987933098889 24.245339977728353 56-57 23.91828177404422 25.91857187582878 26.10649176943897 24.05665458068803 58-59 24.182314600970194 25.730512855556626 26.204624261684224 23.882548281788957 60-61 24.190809529753462 25.72283780550898 26.233737293783722 23.85261537095384 62-63 24.017173391415696 25.82523830324499 26.215271752463813 23.942316552875496 64-65 23.867015722470715 25.796963214504764 26.162514398551917 24.1735066644726 66-67 23.917065085358338 26.008556776907472 25.963822186585606 24.110555951148584 68-69 23.760123026107145 25.67702056618338 26.42635487287502 24.13650153483446 70-71 23.930532737445574 25.556622262305506 26.158745112456582 24.354099887792344 72-73 23.920737411679834 25.846424905464104 26.046321480558284 24.186516202297774 74-75 23.765472507614334 25.98992334803344 25.879068208881094 24.36553593547113 76-77 24.104034591704384 25.875167554917066 25.822180691798113 24.198617161580437 78-79 23.928825445421992 25.903845179326655 26.001205897613232 24.166123477638124 80-81 23.92439065109513 26.041830598820347 25.986492090267348 24.047286659817168 82-83 24.177358648627624 26.036548806539052 25.822737091533575 23.963355453299744 84-85 24.208332216358635 25.780513993458996 25.55948464702323 24.451669143159137 86-87 23.919092953045386 25.642392539561804 26.140339021069114 24.298175486323697 88-89 24.010121517192314 25.819848309659683 25.890149999006866 24.279880174141137 90-91 23.93294919208127 26.02523769130131 25.91982253855438 24.121990578063034 92-93 23.908370243710515 25.78653251141879 25.986161819286508 24.31893542558419 94-95 23.99209791746352 25.77205313883624 26.038407941241097 24.19744100245914 96-97 23.89498917506711 25.69404634674971 26.054925314545386 24.3560391636378 98-99 23.601277876964488 25.73058213676979 25.906030517486556 24.762109468779165 100-101 23.701902093359433 25.67386226185836 25.967702577544323 24.656533067237888 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 58.0 1 44.0 2 65.0 3 114.0 4 95.5 5 81.5 6 187.5 7 404.0 8 613.0 9 670.0 10 572.0 11 522.5 12 585.0 13 687.0 14 805.0 15 889.5 16 1021.0 17 1179.0 18 1322.0 19 1485.0 20 1694.0 21 2089.5 22 2700.0 23 3564.5 24 4810.5 25 6224.5 26 8340.5 27 11307.0 28 14516.5 29 19141.0 30 24863.5 31 31756.5 32 40182.5 33 47172.0 34 54191.0 35 62414.5 36 71459.0 37 80747.5 38 88224.5 39 95346.5 40 103979.5 41 115384.5 42 126917.5 43 136297.5 44 142795.5 45 148902.0 46 154664.5 47 160803.0 48 169441.5 49 172572.0 50 167111.5 51 163356.5 52 168076.0 53 171383.5 54 173004.0 55 167330.0 56 152719.0 57 139147.5 58 129492.0 59 118255.0 60 103945.0 61 90123.5 62 75711.0 63 61643.0 64 47113.0 65 34440.0 66 24716.0 67 20322.0 68 15514.5 69 10683.0 70 8660.0 71 6105.5 72 4569.0 73 3813.5 74 2782.0 75 1930.5 76 1189.5 77 495.0 78 218.5 79 105.0 80 56.5 81 37.5 82 22.0 83 8.5 84 5.5 85 5.5 86 7.0 87 3.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26395562443528264 2 0.020368719295831113 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.3935040300624108E-4 12-13 3.5902560450936165E-5 14-15 0.0 16-17 6.223110478162267E-4 18-19 0.0 20-21 5.983760075156027E-5 22-23 1.1967520150312053E-5 24-25 0.0024054715502127228 26-27 0.0024054715502127228 28-29 0.002225958747958042 30-31 0.0010292067329268366 32-33 0.001591680179991503 34-35 0.0016395502605927514 36-37 5.983760075156027E-5 38-39 1.5557776195405668E-4 40-41 6.103435276659147E-4 42-43 0.0037697688473482963 44-45 0.0039014115690017294 46-47 0.013415590088499811 48-49 0.014516601942328521 50-51 0.005708507111698849 52-53 0.007168544570036919 54-55 4.1886320526092187E-4 56-57 0.003135490279381758 58-59 0.0012924921762337018 60-61 0.0011847844948808931 62-63 0.0 64-65 8.97564011273404E-4 66-67 0.0 68-69 0.0 70-71 1.6754528210436874E-4 72-73 0.0015438100993902548 74-75 0.0 76-77 0.19323954786708872 78-79 0.10341134161884645 80-81 0.0012565896157827655 82-83 0.0 84-85 5.505059269143545E-4 86-87 0.12295430202430603 88-89 0.5851518977495078 90-91 0.3114906144723221 92-93 0.2932999838438478 94-95 0.0021302185867555453 96-97 0.0028722048360748926 98-99 0.0025849843524674036 100-101 6.103435276659147E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4177975.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.69516655864554 #Duplication Level Percentage of deduplicated Percentage of total 1 63.33848235161644 23.87554641816013 2 16.189882881105856 12.205606635364985 3 7.2226572485084874 8.167778039356078 4 3.865594978256251 5.828569862145326 5 2.347433785137506 4.424345375807501 6 1.5650802529756047 3.53975764881375 7 1.040847314218643 2.7464439010113426 8 0.7723906832436711 2.3292316362572967 9 0.5619034045630791 1.9062938182387728 >10 2.801839127494935 18.29628457079734 >50 0.17120400869862945 4.492970875758724 >100 0.11175421613065478 8.182448349002971 >500 0.008133766064618575 2.0626761513343848 >1k 0.0027959819857524804 1.9420467179513896 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 4549 0.10888049832753907 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.787008060124821E-5 2.3935040300624105E-5 3 0.0 0.0 0.0 4.787008060124821E-5 2.3935040300624105E-5 4 0.0 0.0 0.0 4.787008060124821E-5 2.3935040300624105E-5 5 0.0 0.0 0.0 4.787008060124821E-5 2.3935040300624105E-5 6 0.0 0.0 0.0 4.787008060124821E-5 2.3935040300624105E-5 7 0.0 0.0 0.0 4.787008060124821E-5 2.3935040300624105E-5 8 0.0 0.0 0.0 4.787008060124821E-5 2.3935040300624105E-5 9 0.0 0.0 0.0 7.180512090187232E-5 7.180512090187232E-5 10-11 0.0 0.0 0.0 1.6754528210436874E-4 7.180512090187232E-5 12-13 0.0 0.0 0.0 1.6754528210436874E-4 8.377264105218437E-5 14-15 0.0 0.0 0.0 2.1541536270561695E-4 1.1967520150312053E-4 16-17 0.0 0.0 0.0 2.8722048360748926E-4 1.4361024180374463E-4 18-19 0.0 2.3935040300624105E-5 0.0 3.1115552390811337E-4 1.4361024180374463E-4 20-21 0.0 2.3935040300624105E-5 0.0 3.7099312465967366E-4 1.4361024180374463E-4 22-23 0.0 3.590256045093616E-5 0.0 5.265708866137303E-4 1.6754528210436874E-4 24-25 0.0 4.787008060124821E-5 0.0 6.82148648567787E-4 1.9148032240499284E-4 26-27 0.0 4.787008060124821E-5 0.0 8.137913702212197E-4 1.9148032240499284E-4 28-29 0.0 4.787008060124821E-5 0.0 0.00140019985758651 2.034478425553049E-4 30-31 0.0 4.787008060124821E-5 0.0 0.003326970601786751 2.27382882855929E-4 32-33 0.0 4.787008060124821E-5 0.0 0.006917226646880367 2.6328544330686516E-4 34-35 0.0 4.787008060124821E-5 0.0 0.011883747509259869 2.6328544330686516E-4 36-37 0.0 4.787008060124821E-5 0.0 0.02017723897342612 2.8722048360748926E-4 38-39 0.0 4.787008060124821E-5 0.0 0.033712504263429054 2.991880037578013E-4 40-41 0.0 4.787008060124821E-5 0.0 0.04990455902680126 3.1115552390811337E-4 42-43 0.0 4.787008060124821E-5 0.0 0.06719762564400218 3.1115552390811337E-4 44-45 0.0 4.787008060124821E-5 0.0 0.08651320316660582 3.350905642087375E-4 46-47 0.0 4.787008060124821E-5 0.0 0.10998151018136777 3.7099312465967366E-4 48-49 0.0 4.787008060124821E-5 0.0 0.13686056043896866 3.829606448099857E-4 50-51 0.0 4.787008060124821E-5 0.0 0.16643230273038973 3.829606448099857E-4 52-53 0.0 4.787008060124821E-5 0.0 0.1966861936703786 4.068956851106098E-4 54-55 0.0 4.787008060124821E-5 0.0 0.23124839186447982 4.308307254112339E-4 56-57 0.0 4.787008060124821E-5 0.0 0.2702625075544971 4.308307254112339E-4 58-59 0.0 4.787008060124821E-5 0.0 0.3143269167479461 4.308307254112339E-4 60-61 0.0 4.787008060124821E-5 0.0 0.363836547609787 4.308307254112339E-4 62-63 0.0 4.787008060124821E-5 0.0 0.4141480023216989 4.308307254112339E-4 64-65 0.0 4.787008060124821E-5 0.0 0.4695217180571928 4.308307254112339E-4 66-67 0.0 4.787008060124821E-5 0.0 0.5334282756598592 4.308307254112339E-4 68-69 0.0 4.787008060124821E-5 0.0 0.6058557076095477 4.308307254112339E-4 70-71 0.0 4.787008060124821E-5 0.0 0.6858226772539329 4.54765765711858E-4 72-73 0.0 4.787008060124821E-5 0.0 0.7708758429622007 5.265708866137303E-4 74-75 0.0 4.787008060124821E-5 0.0 0.8587413759057917 5.265708866137303E-4 76-77 0.0 4.787008060124821E-5 0.0 0.957198164182409 5.265708866137303E-4 78-79 0.0 4.787008060124821E-5 0.0 1.0689748023863235 5.265708866137303E-4 80-81 0.0 4.787008060124821E-5 0.0 1.198930103698562 5.744409672149785E-4 82-83 0.0 4.787008060124821E-5 0.0 1.3322482781730383 6.941161687180991E-4 84-85 0.0 4.787008060124821E-5 0.0 1.4709638042353053 6.941161687180991E-4 86-87 0.0 4.787008060124821E-5 0.0 1.620521903553755 6.941161687180991E-4 88-89 0.0 4.787008060124821E-5 0.0 1.7911189032964536 7.180512090187232E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8020 0.0 32.623844 1 GGTATCA 5505 0.0 32.49206 1 ATCAACG 8940 0.0 29.022644 3 TCAACGC 8980 0.0 28.840546 4 CAACGCA 9280 0.0 27.908201 5 TATCAAC 9685 0.0 26.944155 2 AACGCAG 9705 0.0 26.881552 6 TACATGG 11985 0.0 25.45508 2 GTACATG 12690 0.0 24.51675 1 ACATGGG 12265 0.0 23.97791 3 ACGCAGA 11275 0.0 23.180468 7 CGCAGAG 11660 0.0 22.374395 8 AGTACAT 8705 0.0 21.311277 2 GGACCGT 580 0.0 21.263391 6 GAGTACA 9745 0.0 21.088646 1 TACCTGG 3340 0.0 20.45585 2 GTGTAGC 2610 0.0 20.413822 1 CATGGGG 8705 0.0 20.270353 4 GTACCTG 3575 0.0 20.093134 1 GTATAGG 1260 0.0 18.500025 1 >>END_MODULE