FastQCFastQC Report
Thu 26 May 2016
SRR936343_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936343_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3259165
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64140.1967988733310526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46350.14221434017608806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60300.040.437561
GTATCAA98450.037.8008731
TCAACGC119900.030.5550824
ATCAACG120450.030.416033
GTACATG160650.029.7965131
CAACGCA123750.029.5279855
TACATGG160600.029.4710882
TATCAAC127300.028.9634822
AACGCAG127100.028.9336936
ACATGGG169350.026.9718363
CATGGGG88450.024.3486714
ACGCAGA149900.024.2809937
CGCAGAG154400.023.48288
GTATACG1652.501165E-622.9562571
GTATAGG7650.022.2810731
GTGTACG4400.021.521491
CATGGGA78950.021.4631064
GAGTACA123950.020.8946861
AGTACAT115900.020.7454092
GCAGAGT174050.020.4780839
Produced by FastQC (version 0.11.5)