##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936343_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3259165 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.827875851636847 33.0 31.0 34.0 30.0 34.0 2 31.91295040294063 34.0 31.0 34.0 30.0 34.0 3 31.984207304631706 34.0 31.0 34.0 30.0 34.0 4 35.48255826262248 37.0 35.0 37.0 33.0 37.0 5 35.47402662952014 37.0 35.0 37.0 33.0 37.0 6 35.56643956350783 37.0 35.0 37.0 33.0 37.0 7 35.50871772371144 37.0 35.0 37.0 33.0 37.0 8 35.51083421673956 37.0 35.0 37.0 33.0 37.0 9 37.19317217753627 39.0 37.0 39.0 34.0 39.0 10-11 37.117181548034544 39.0 37.0 39.0 33.5 39.0 12-13 37.028240975832766 39.0 37.0 39.0 33.0 39.0 14-15 38.29058715959456 40.0 38.0 41.0 33.5 41.0 16-17 38.24697752338406 40.0 38.0 41.0 33.0 41.0 18-19 38.19501237280101 40.0 38.0 41.0 33.5 41.0 20-21 38.118197759242015 40.0 38.0 41.0 33.0 41.0 22-23 37.96694260646515 40.0 38.0 41.0 33.0 41.0 24-25 38.11086980867799 40.0 38.0 41.0 33.0 41.0 26-27 38.06883772377281 40.0 38.0 41.0 33.0 41.0 28-29 37.9474253374714 40.0 38.0 41.0 33.0 41.0 30-31 37.80313684640085 40.0 38.0 41.0 33.0 41.0 32-33 37.63312596938173 40.0 37.5 41.0 32.5 41.0 34-35 37.38388452256943 40.0 37.0 41.0 32.0 41.0 36-37 37.18751029788305 40.0 37.0 41.0 31.0 41.0 38-39 37.029324535578894 40.0 37.0 41.0 31.0 41.0 40-41 36.8467423404461 40.0 36.5 41.0 30.5 41.0 42-43 36.64972040384577 40.0 36.0 41.0 30.0 41.0 44-45 36.466577789096284 40.0 36.0 41.0 30.0 41.0 46-47 36.18916363547105 39.5 35.5 41.0 29.5 41.0 48-49 36.021321565493 39.0 35.0 41.0 29.0 41.0 50-51 35.70593495573253 39.0 35.0 40.5 28.5 41.0 52-53 35.70253193686113 39.0 35.0 40.5 28.5 41.0 54-55 35.76792828838062 39.0 35.0 41.0 28.0 41.0 56-57 35.585415129335274 39.0 35.0 41.0 28.0 41.0 58-59 35.33371906607981 38.5 35.0 41.0 28.0 41.0 60-61 35.02536217098552 38.0 35.0 40.0 27.5 41.0 62-63 34.70598404806139 37.0 34.0 40.0 27.0 41.0 64-65 34.36369959790314 37.0 34.0 40.0 26.0 41.0 66-67 33.99838424872628 36.0 34.0 39.0 26.0 41.0 68-69 33.619991470207864 36.0 34.0 39.0 26.0 41.0 70-71 33.22036319118547 35.0 33.5 38.5 25.5 40.5 72-73 32.83292944665274 35.0 33.0 37.5 25.0 40.0 74-75 32.43798488263098 35.0 33.0 37.0 24.0 39.0 76-77 32.070578660485126 35.0 33.0 36.5 23.5 39.0 78-79 31.712632836938297 35.0 32.0 36.0 22.5 38.5 80-81 31.36011662496376 35.0 32.0 35.5 20.5 37.0 82-83 31.045451825851103 35.0 32.0 35.0 19.5 37.0 84-85 30.76565117138899 35.0 31.5 35.0 18.5 36.0 86-87 30.480567108446486 34.5 31.0 35.0 17.5 36.0 88-89 30.19275090398921 34.0 31.0 35.0 12.5 36.0 90-91 29.87496950906137 34.0 31.0 35.0 7.0 35.0 92-93 29.591025155216137 34.0 30.5 35.0 2.0 35.0 94-95 29.24719398987164 34.0 30.0 35.0 2.0 35.0 96-97 28.866132583038905 34.0 30.0 35.0 2.0 35.0 98-99 28.413697833647575 34.0 29.5 35.0 2.0 35.0 100-101 25.220895842953638 30.5 23.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30783489384734963 1101 2 0.24754743441468108 1101 3 0.3446096197103472 1101 4 0.14434912156180246 1101 5 0.4177435603255546 1101 6 0.3511157950364989 1101 7 0.4109758870748479 1101 8 0.3193089840724497 1101 9 0.40859079604328485 1101 10-11 0.5048501702144463 1101 12-13 0.408481034957326 1101 14-15 0.5679216030283314 1101 16-17 0.6071592911884025 1101 18-19 0.6536911197166191 1101 20-21 0.6882390771389666 1101 22-23 0.7202813899156411 1101 24-25 0.6843575727623943 1101 26-27 0.5918548079437684 1101 28-29 0.5511656007584307 1101 30-31 0.7082659444092556 1101 32-33 0.6718855550974752 1101 34-35 0.5018466319140487 1101 36-37 0.459359497150416 1101 38-39 0.4439813622770288 1101 40-41 0.47937398111601937 1101 42-43 0.40636283777222104 1101 44-45 0.30683668722358703 1101 46-47 0.25765980159953017 1101 48-49 0.3047558106471726 1101 50-51 0.4610159757281451 1101 52-53 0.4190728927810312 1101 54-55 0.37023456540035227 1101 56-57 0.3904104585595647 1101 58-59 0.49057829617319726 1101 60-61 0.3194079733805282 1101 62-63 0.35584729894649314 1101 64-65 0.27696127105599544 1101 66-67 0.34854210094611204 1101 68-69 0.3738736308039776 1101 70-71 0.47825796842906243 1101 72-73 0.5107509008533029 1101 74-75 0.40153991777939524 1101 76-77 0.29865958318345065 1101 78-79 0.46735657768040184 1101 80-81 0.453234532473914 1101 82-83 0.6258199370863338 1101 84-85 0.5877093838939871 1101 86-87 0.599151263140218 1101 88-89 0.4602705558362601 1101 90-91 0.38210314689662184 1101 92-93 0.45903438688213427 1101 94-95 0.7460547826615986 1101 96-97 0.6669418051567284 1101 98-99 0.612774666755822 1101 100-101 0.5758543876324964 1102 1 0.4872338828349996 1102 2 0.43035015625030226 1102 3 0.34175650416570846 1102 4 0.3147290226572821 1102 5 0.49981966607127504 1102 6 0.4041229422086232 1102 7 0.39959075308825476 1102 8 0.3221432706235774 1102 9 0.4112618979839766 1102 10-11 0.5076762190809063 1102 12-13 0.5804085747139567 1102 14-15 0.6076161287599788 1102 16-17 0.5881034344561229 1102 18-19 0.6365551665380451 1102 20-21 0.5404221943311498 1102 22-23 0.5773847584748779 1102 24-25 0.6457569592510168 1102 26-27 0.6354372593218187 1102 28-29 0.6421170271854493 1102 30-31 0.6019092631239644 1102 32-33 0.3857043364954791 1102 34-35 0.4193408589969394 1102 36-37 0.390342802109501 1102 38-39 0.3087821279894243 1102 40-41 0.23101537247403314 1102 42-43 0.2966661022489774 1102 44-45 0.2834485275223315 1102 46-47 0.2975745873281994 1102 48-49 0.2242083838121971 1102 50-51 0.1704804869980876 1102 52-53 0.23053935755903865 1102 54-55 0.2140179901327599 1102 56-57 0.28422278056673633 1102 58-59 0.25937780400422383 1102 60-61 0.29494342288352016 1102 62-63 0.38015448351507786 1102 64-65 0.2519223708784324 1102 66-67 0.2925022436280429 1102 68-69 0.31518476946121154 1102 70-71 0.42357968270431456 1102 72-73 0.3159190019231559 1102 74-75 0.34251076160597194 1102 76-77 0.2923258497340129 1102 78-79 0.3668114521668535 1102 80-81 0.2642966969190219 1102 82-83 0.27226086504189695 1102 84-85 0.2891286846583938 1102 86-87 0.24835959549287523 1102 88-89 0.2900021903759509 1102 90-91 0.39033932785370595 1102 92-93 0.42102414077149675 1102 94-95 0.383887722416663 1102 96-97 0.4819625693523477 1102 98-99 0.35479091199299617 1102 100-101 0.4222209149123053 1103 1 0.2513452249719279 1103 2 0.20610897536715456 1103 3 0.2986564845313069 1103 4 0.2232951578526965 1103 5 0.2242559323137101 1103 6 0.18317228495975257 1103 7 0.24721750822718036 1103 8 0.22529685692178703 1103 9 0.24220419802777826 1103 10-11 0.28524442343974954 1103 12-13 0.33821928905361887 1103 14-15 0.3937346097624399 1103 16-17 0.4634627691017741 1103 18-19 0.38096892874032307 1103 20-21 0.45836274741702 1103 22-23 0.49065937321276465 1103 24-25 0.47790576784585426 1103 26-27 0.5163141113741219 1103 28-29 0.5226118895181173 1103 30-31 0.5039229216163434 1103 32-33 0.5852688556289465 1103 34-35 0.4785886601961451 1103 36-37 0.4558097987011749 1103 38-39 0.5635928070878578 1103 40-41 0.5763461617264625 1103 42-43 0.44488277034207613 1103 44-45 0.5965070380267363 1103 46-47 0.6594666132456126 1103 48-49 0.5606541047721763 1103 50-51 0.5505265271428783 1103 52-53 0.5980781606663044 1103 54-55 0.6426859724718668 1103 56-57 0.5813012829153053 1103 58-59 0.6422710835023935 1103 60-61 0.5792143826027996 1103 62-63 0.6309904557449997 1103 64-65 0.527243947067447 1103 66-67 0.6349297851497582 1103 68-69 0.5361876592417616 1103 70-71 0.505482180300362 1103 72-73 0.5744595872295832 1103 74-75 0.5557372972423948 1103 76-77 0.49791838060784244 1103 78-79 0.530553013151799 1103 80-81 0.5128379618776613 1103 82-83 0.6186690289236694 1103 84-85 0.6165839912318916 1103 86-87 0.7084935876693237 1103 88-89 0.5647440483940898 1103 90-91 0.6602930359858732 1103 92-93 0.6126259048465919 1103 94-95 0.7087642522634674 1103 96-97 0.7208929226204681 1103 98-99 0.7392152969077408 1103 100-101 0.9849502975363684 1104 1 0.23620287230894377 1104 2 0.1292463394752481 1104 3 0.19578614494707836 1104 4 0.22037212395669314 1104 5 0.2627797953949198 1104 6 0.2789683831800289 1104 7 0.2931819521701513 1104 8 0.2619979049264245 1104 9 0.23224737375430493 1104 10-11 0.23855162167478738 1104 12-13 0.3229212541073281 1104 14-15 0.4753981295744225 1104 16-17 0.3739784051485202 1104 18-19 0.2129313897447318 1104 20-21 0.29904911783522437 1104 22-23 0.39832087407638994 1104 24-25 0.4482329887190417 1104 26-27 0.38144031965228464 1104 28-29 0.3488124255837093 1104 30-31 0.4877580112266813 1104 32-33 0.45032358449330445 1104 34-35 0.42457056888255096 1104 36-37 0.35844163350750335 1104 38-39 0.28194747221490957 1104 40-41 0.3295111213294959 1104 42-43 0.3414496579087398 1104 44-45 0.42301779834924247 1104 46-47 0.5128197839667763 1104 48-49 0.33679954587397276 1104 50-51 0.31819418567893365 1104 52-53 0.3633361047239134 1104 54-55 0.5428936888876521 1104 56-57 0.4733277573057535 1104 58-59 0.446307539803918 1104 60-61 0.3727186983749604 1104 62-63 0.437708440349617 1104 64-65 0.4491002032266209 1104 66-67 0.5049129325725943 1104 68-69 0.43327002681279225 1104 70-71 0.40246185998136497 1104 72-73 0.5034490807760292 1104 74-75 0.3651399309977279 1104 76-77 0.4481281375778323 1104 78-79 0.34208262459426564 1104 80-81 0.39979467932212387 1104 82-83 0.46819111912336453 1104 84-85 0.24425504669055442 1104 86-87 0.31244236015593074 1104 88-89 0.39427122797606273 1104 90-91 0.5530091576845244 1104 92-93 0.3906824450028985 1104 94-95 0.09711994967087989 1104 96-97 0.3782065690068279 1104 98-99 0.35226182034350373 1104 100-101 0.6005178656878805 1105 1 0.20857438618989477 1105 2 0.20415898797280008 1105 3 0.20704972413518874 1105 4 0.11839639179228101 1105 5 0.08286111170264832 1105 6 0.1360549723420874 1105 7 0.2507704154189909 1105 8 0.22412229346591062 1105 9 0.13038599264763917 1105 10-11 0.23664577071232884 1105 12-13 0.2113305038373312 1105 14-15 0.22625576250752744 1105 16-17 0.19842729657854363 1105 18-19 0.345480147765187 1105 20-21 0.3381476503299794 1105 22-23 0.4153083589884119 1105 24-25 0.2860895416699307 1105 26-27 0.28970535999280145 1105 28-29 0.3252402255964242 1105 30-31 0.46368711357087733 1105 32-33 0.4240990752499272 1105 34-35 0.22182614349661378 1105 36-37 0.23215359589871554 1105 38-39 0.26066546980082705 1105 40-41 0.30409024590862543 1105 42-43 0.37493863573733677 1105 44-45 0.32859241241442305 1105 46-47 0.36935049993144986 1105 48-49 0.33850845890363956 1105 50-51 0.40848705144350106 1105 52-53 0.42978359852990167 1105 54-55 0.5370141386684821 1105 56-57 0.6605871436217399 1105 58-59 0.6786720655646761 1105 60-61 0.5428317788653985 1105 62-63 0.5033650060061774 1105 64-65 0.5132066767670551 1105 66-67 0.5118279951480318 1105 68-69 0.5324600401537509 1105 70-71 0.5529446755585141 1105 72-73 0.4619809402512871 1105 74-75 0.5356786515364504 1105 76-77 0.5795651492035248 1105 78-79 0.5040640743115681 1105 80-81 0.6528440408620462 1105 82-83 0.5939804590636477 1105 84-85 0.47204235372313974 1105 86-87 0.51506925806585 1105 88-89 0.5104646083203832 1105 90-91 0.5420282797225155 1105 92-93 0.6180568176602925 1105 94-95 0.6204475654513253 1105 96-97 0.6015341847872726 1105 98-99 0.6905853067801964 1105 100-101 0.7971985085949349 1106 1 0.08451236489923275 1106 2 0.07628995340630595 1106 3 0.13459738817245892 1106 4 0.11506226975146205 1106 5 0.0897880740568624 1106 6 0.08062083330517567 1106 7 0.08864925884056163 1106 8 -0.054674694338004315 1106 9 0.03995390313934166 1106 10-11 0.05934221719630983 1106 12-13 0.040426756143745024 1106 14-15 0.08679233909491302 1106 16-17 0.0848486974341256 1106 18-19 0.06746003683514346 1106 20-21 0.05586940765913084 1106 22-23 0.06421888734466563 1106 24-25 0.10087291927085573 1106 26-27 0.11289691482406994 1106 28-29 0.07179257411181794 1106 30-31 0.08554508623716828 1106 32-33 0.06591779027880307 1106 34-35 -0.04293196213908601 1106 36-37 -0.04893519812311098 1106 38-39 0.058879325058562415 1106 40-41 -0.012740160875594597 1106 42-43 0.08648503562091747 1106 44-45 0.11584084527486738 1106 46-47 0.08070294442477177 1106 48-49 0.030496523870915837 1106 50-51 0.031758254622324955 1106 52-53 0.027629260694268964 1106 54-55 0.07220521138842173 1106 56-57 -0.030276791006627946 1106 58-59 0.10704192938488433 1106 60-61 0.06548351341090353 1106 62-63 -0.05773860682953824 1106 64-65 -0.09078784893647196 1106 66-67 -0.047644715017511885 1106 68-69 0.03273880937016571 1106 70-71 0.12755912534617408 1106 72-73 0.2170143381253311 1106 74-75 0.14023665875034652 1106 76-77 0.19300789070631197 1106 78-79 0.18354418277550977 1106 80-81 0.1992701961796577 1106 82-83 0.14097503919910537 1106 84-85 0.25221391426421746 1106 86-87 0.1949744909733866 1106 88-89 0.10749184761090547 1106 90-91 0.12666842360053465 1106 92-93 0.17466624193714964 1106 94-95 0.22083447646179266 1106 96-97 0.258287131609503 1106 98-99 0.2900513740291757 1106 100-101 0.4930400761455118 1107 1 0.02448224698293089 1107 2 0.10710353434114595 1107 3 0.1986926671351199 1107 4 0.07787566609410135 1107 5 0.01593787797357038 1107 6 0.138091341601843 1107 7 0.10955731011402747 1107 8 0.17854825102339333 1107 9 0.2346935076023371 1107 10-11 0.22277111602348043 1107 12-13 0.15045908962562748 1107 14-15 0.21718066919444112 1107 16-17 0.06356440179916234 1107 18-19 0.14417095282342274 1107 20-21 0.21129351397689078 1107 22-23 0.11963379849130007 1107 24-25 0.23194721372802007 1107 26-27 0.1809662640929517 1107 28-29 0.1242276643542155 1107 30-31 0.08780301159074355 1107 32-33 0.10668379509978365 1107 34-35 0.043923948266652246 1107 36-37 0.17182237836499326 1107 38-39 0.24787085700621247 1107 40-41 0.28108320964480527 1107 42-43 -0.23491810846178396 1107 44-45 0.05670653906717149 1107 46-47 0.20645712639089453 1107 48-49 0.09304934444661228 1107 50-51 0.08945399295323853 1107 52-53 0.18813011167807758 1107 54-55 0.2397460506905773 1107 56-57 0.22388399522757396 1107 58-59 0.2438274467952155 1107 60-61 0.19699167989377742 1107 62-63 0.25910718774000685 1107 64-65 0.09210175138237986 1107 66-67 0.11819788753044946 1107 68-69 0.2574784918945383 1107 70-71 0.38523979094340177 1107 72-73 0.19904644568840268 1107 74-75 0.21914448594173308 1107 76-77 0.18645555640708267 1107 78-79 0.14559068347909587 1107 80-81 0.13500806102189244 1107 82-83 0.29483837714494854 1107 84-85 0.30634993897018603 1107 86-87 0.0571132188989516 1107 88-89 0.1784626120177606 1107 90-91 0.37210602305704654 1107 92-93 0.15734356122678506 1107 94-95 0.26906842962542754 1107 96-97 0.5290251697939148 1107 98-99 0.3853316568501377 1107 100-101 0.4943636182861404 1108 1 0.10558453816693358 1108 2 0.11947359732714702 1108 3 0.1452023000361038 1108 4 0.18561286430676205 1108 5 0.12775629500843166 1108 6 0.0933720727055487 1108 7 0.09862709638825606 1108 8 0.18060926982043668 1108 9 0.2616315927674151 1108 10-11 0.1637111262545119 1108 12-13 0.1431518710891595 1108 14-15 0.11933938969711022 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2316 58-59 -1.5914655924063936 2316 60-61 -1.632171254116109 2316 62-63 -1.6009672732676066 2316 64-65 -1.3554730230544294 2316 66-67 -1.414144016341055 2316 68-69 -1.512864284042827 2316 70-71 -1.4428171857625394 2316 72-73 -1.3397208380999999 2316 74-75 -1.3081595117214633 2316 76-77 -1.5374718563220178 2316 78-79 -1.674181097733321 2316 80-81 -1.5065016386754913 2316 82-83 -1.5784536779703942 2316 84-85 -1.652828961204328 2316 86-87 -1.7270341185485272 2316 88-89 -1.607763452982077 2316 90-91 -1.6662929610741593 2316 92-93 -1.7040818203183115 2316 94-95 -1.678622924190055 2316 96-97 -1.8687470080489028 2316 98-99 -1.6457753359912992 2316 100-101 -1.7286040498234385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21605.0 3 5099.0 4 4397.0 5 3688.0 6 3539.0 7 3840.0 8 4552.0 9 5559.0 10 6509.0 11 7879.0 12 10289.0 13 15352.0 14 15886.0 15 13302.0 16 15855.0 17 19947.0 18 18161.0 19 13463.0 20 12885.0 21 13620.0 22 14394.0 23 16049.0 24 18786.0 25 22110.0 26 26224.0 27 31588.0 28 38686.0 29 47427.0 30 58247.0 31 73368.0 32 94541.0 33 124744.0 34 176925.0 35 272984.0 36 467547.0 37 757592.0 38 684867.0 39 117604.0 40 55.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.04620751789225 17.29115217968533 12.400547012534936 24.26209328988748 2 16.345473437040663 19.0890411337228 38.90200215663133 25.6634832726052 3 20.30988355661851 23.13917511062996 27.826031188427038 28.724910144324493 4 13.017393796460116 14.491721024198384 35.0720379210906 37.4188472582509 5 14.518216620863777 37.24959280638941 32.97123684982503 15.260953722921776 6 35.193425669028755 33.70777587329525 16.40115398140153 14.697644476274462 7 31.250186023605437 31.203914090079916 19.11619993280137 18.429699953513275 8 28.248337926933225 34.02992191800747 18.591897466677224 19.12984268838209 9 27.764451071484576 15.178768976491567 18.83169469571176 38.2250852563121 10-11 26.696611617071813 25.4264416939506 25.932104084834684 21.944842604142902 12-13 26.858006255517612 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26.706481787626483 23.851122584483818 38-39 23.810617455138914 25.54887761352493 26.756456146051278 23.88404878528488 40-41 23.830279529151742 25.4623103089325 26.924198808079012 23.78321135383674 42-43 23.857871150350697 25.299326174077425 26.70022307737013 24.142579598201756 44-45 23.841021925697557 25.501210231159625 26.712428138911314 23.9453397042315 46-47 23.98905213142993 25.728909656802113 26.446216768537784 23.835821443230174 48-49 24.065258508576964 25.805523439506985 26.211866411355643 23.917351640560405 50-51 24.044269332556507 25.884075059731 26.21487984635335 23.856775761359145 52-53 24.274864812229744 25.73271393698829 26.071899586121656 23.920521664660317 54-55 24.085036065104667 25.724971508073946 26.194425326485405 23.995567100335983 56-57 23.938607577958475 26.032824684745076 26.021678179582995 24.00688955771346 58-59 23.995706743840856 25.693352852992668 26.24558078900453 24.06535961416195 60-61 23.964635194456328 25.914401446041307 26.353501601359707 23.767461758142655 62-63 23.877222789096507 25.848907302925202 26.38983332251656 23.884036585461732 64-65 23.861523712197908 25.909962320866853 26.29008399557311 23.938429971362133 66-67 23.755832954270627 26.207920378488268 26.093238173821536 23.94300849341957 68-69 23.835295271304968 25.733976752777316 26.432537477466035 23.998190498451685 70-71 23.96100502698478 25.648675271582043 26.227433678794014 24.16288602263916 72-73 23.78095068364072 26.041895348579068 25.97546453775626 24.20168943002395 74-75 23.69066375064027 26.318803467036965 25.934486853673512 24.05604592864925 76-77 23.90743741616085 26.103701310083792 25.974815841010834 24.014045432744524 78-79 23.866448713440274 26.132911487052844 26.060652684914775 23.9399871145921 80-81 23.864069973261895 26.137926558169443 25.922763680518585 24.075239788050073 82-83 24.02004572806448 26.192437139328757 25.973787304372227 23.813729828234536 84-85 23.79550430143998 26.28269248558478 25.830902531528476 24.090900681446765 86-87 23.881676017446566 25.912730165631377 26.13438913120231 24.07120468571975 88-89 24.014869475756612 26.13201809896701 25.736041517096076 24.117070908180306 90-91 23.808164346832818 26.207104820830967 25.872848859609103 24.111881972727105 92-93 23.84150866347093 26.06412738870791 25.89870132754144 24.19566262027972 94-95 23.836907592418836 25.846717212284503 26.174932773547145 24.141442421749517 96-97 23.978997650444605 25.700658874272587 26.065114311942782 24.25522916334003 98-99 23.67768613509803 25.89113503098585 25.980553299036295 24.450625534879826 100-101 23.70527329463084 25.863708565462506 25.906032034519676 24.52498610538698 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 244.0 1 181.0 2 257.5 3 430.0 4 357.5 5 347.0 6 685.0 7 1282.0 8 1945.0 9 2195.0 10 1951.0 11 1786.0 12 1978.5 13 2277.5 14 2539.5 15 2855.0 16 3182.5 17 3406.5 18 3587.5 19 3798.5 20 3956.0 21 4124.5 22 4435.0 23 5032.0 24 5829.0 25 7039.0 26 9002.5 27 11667.0 28 14124.5 29 17444.5 30 22228.0 31 28253.5 32 36027.5 33 40373.0 34 43025.0 35 48079.5 36 54491.5 37 61036.0 38 66978.0 39 70604.5 40 74471.0 41 80519.5 42 83952.0 43 90471.0 44 99901.0 45 108328.5 46 115318.0 47 121366.0 48 126982.5 49 128162.0 50 128281.5 51 128730.5 52 130416.0 53 134483.5 54 137063.5 55 132071.0 56 122179.5 57 112516.0 58 106917.0 59 100004.0 60 85985.5 61 72418.5 62 60932.0 63 47630.5 64 35759.5 65 27705.5 66 19771.5 67 14905.0 68 11505.5 69 8498.5 70 6384.5 71 4192.5 72 3084.5 73 2589.5 74 2063.0 75 1546.5 76 1083.5 77 616.5 78 352.5 79 224.5 80 140.5 81 103.0 82 76.5 83 62.5 84 54.0 85 34.5 86 27.0 87 29.0 88 29.0 89 29.5 90 31.0 91 27.0 92 27.5 93 30.0 94 30.5 95 28.5 96 31.5 97 30.5 98 20.0 99 14.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04510357714322533 2 0.01162874539951184 3 0.010063927416991775 4 0.0057376659359068966 5 0.008376378612313277 6 0.0057376659359068966 7 0.005216059941733542 8 0.008683205667709366 9 0.014175409959299392 10-11 0.0022551788571612665 12-13 0.008652522962169758 14-15 0.008207623731845427 16-17 0.017903358682361894 18-19 0.011337259696885552 20-21 0.0033750976093569977 22-23 0.00971107630328627 24-25 0.020342633772760816 26-27 0.0072104358018081315 28-29 0.017458459452037563 30-31 0.013837900198363692 32-33 0.012303764921383237 34-35 0.26677078331413107 36-37 0.47320402618462093 38-39 0.4699976834557318 40-41 0.49083124051712634 42-43 0.4808440198639835 44-45 0.49673766133350106 46-47 0.5014167739282914 48-49 0.5088419886688769 50-51 0.5191667190829553 52-53 0.507660704505602 54-55 0.49524955011483 56-57 0.49080055781158666 58-59 0.489051643595829 60-61 0.4856305219281626 62-63 0.48311454007391463 64-65 0.49877806125188506 66-67 0.47308129536246246 68-69 0.49960649430145454 70-71 0.4807979958056741 72-73 0.4891130090069082 74-75 0.5030736400274304 76-77 0.4901715623480247 78-79 0.4897420044704702 80-81 0.4921659382080993 82-83 0.4933779050769139 84-85 0.4929176644938197 86-87 0.49460521329849827 88-89 0.5077680939749905 90-91 0.5319767486457421 92-93 0.5194888874911212 94-95 0.4915983081556165 96-97 0.46475094080845863 98-99 0.45599102837690025 100-101 0.4633702190591762 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3259165.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.52449368337316 #Duplication Level Percentage of deduplicated Percentage of total 1 63.187785167972756 21.183385047300426 2 14.34477886495715 9.618028968952816 3 6.793281988028554 6.832240172911078 4 3.9597071610236774 5.30988710830983 5 2.5392880757384457 4.256417352767915 6 1.7281500823682703 3.4761213912165525 7 1.264460711661315 2.9673283598674294 8 0.9294111106286187 2.4926429526020764 9 0.7284095676653479 2.1977605755094975 >10 4.113339020050954 24.877622993354446 >50 0.26880293552150275 6.188904623364529 >100 0.13452366804874516 7.903115246451884 >500 0.0055881866640829685 1.2621571079710037 >1k 0.0023818500532350865 1.2374035095548515 >5k 9.160961743211871E-5 0.1969845898658464 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6414 0.1967988733310526 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4635 0.14221434017608806 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5341352769804537E-4 2 0.0 0.0 0.0 0.0 1.5341352769804537E-4 3 0.0 0.0 0.0 0.0 1.5341352769804537E-4 4 0.0 0.0 0.0 0.0 1.5341352769804537E-4 5 0.0 0.0 0.0 0.0 1.8409623323765442E-4 6 0.0 0.0 0.0 0.0 2.147789387772635E-4 7 0.0 0.0 0.0 0.0 2.147789387772635E-4 8 0.0 0.0 0.0 0.0 2.147789387772635E-4 9 0.0 0.0 0.0 0.0 2.147789387772635E-4 10-11 0.0 0.0 0.0 1.5341352769804536E-5 2.914857026262862E-4 12-13 0.0 0.0 0.0 9.204811661882721E-5 6.443368163317905E-4 14-15 0.0 0.0 0.0 1.227308221584363E-4 7.670676384902268E-4 16-17 0.0 0.0 0.0 3.6819246647530883E-4 8.59115755109054E-4 18-19 0.0 0.0 0.0 6.136541107921815E-4 8.897984606486631E-4 20-21 0.0 0.0 0.0 0.0010278706355769038 9.665052244976857E-4 22-23 0.0 0.0 0.0 0.002117106682233026 0.0010125292828070994 24-25 0.0 0.0 0.0 0.0030836119067307113 0.0010125292828070994 26-27 0.0 0.0 0.0 0.003467145725975825 0.001089236046656122 28-29 0.0 0.0 0.0 0.004709795300329993 0.0011506014577353403 30-31 0.0 0.0 0.0 0.00791613802921914 0.0011659428105051447 32-33 0.0 0.0 0.0 0.013362318262499751 0.001227308221584363 34-35 0.0 0.0 0.0 0.020879581119703974 0.0013960631020522127 36-37 0.0 0.0 0.0 0.03453338508483001 0.0014114044548220173 38-39 0.0 0.0 0.0 0.05794428941155173 0.0015341352769804536 40-41 0.0 0.0 0.0 0.08357968988989511 0.0015648179825200627 42-43 0.0 0.0 0.0 0.10939918660147614 0.0015955006880596717 44-45 0.0 0.0 0.0 0.1397136996746099 0.0016722074519086943 46-47 0.0 0.0 0.0 0.17555109974487332 0.001748914215757717 48-49 0.0 0.0 0.0 0.21596022294053846 0.0018409623323765443 50-51 0.0 0.0 0.0 0.263165565413227 0.002025058565614199 52-53 0.0 0.0 0.0 0.30733332003749425 0.0023318856210102893 54-55 0.0 0.0 0.0 0.3549068549766581 0.0023318856210102893 56-57 0.0 0.0 0.0 0.411347691816769 0.002377909679319703 58-59 0.0 0.0 0.0 0.47765301848786423 0.0024239337376291167 60-61 0.0 0.0 0.0 0.5485300682843612 0.002454616443168726 62-63 0.0 0.0 0.0 0.6200821376027295 0.002546664559787553 64-65 0.0 0.0 0.0 0.6970343630960691 0.0027154194402554026 66-67 0.0 0.0 0.0 0.7881159744903986 0.002776784851334621 68-69 0.0 0.0 0.0 0.8929894620247825 0.002838150262413839 70-71 0.0 0.0 0.0 1.008310410795403 0.0028534916151836437 72-73 0.0 0.0 0.0 1.1259479038342644 0.0030836119067307118 74-75 0.0 0.0 0.0 1.2453956764999625 0.00331373219827778 76-77 0.0 0.0 0.0 1.3796171718829824 0.0033290735510475844 78-79 0.0 0.0 0.0 1.5410082030213261 0.0034211216676664114 80-81 0.0 0.0 1.5341352769804536E-5 1.7186610680956624 0.0035131697842852388 82-83 0.0 0.0 3.068270553960907E-5 1.904644287724003 0.003635900606443675 84-85 0.0 0.0 3.068270553960907E-5 2.0907195554689624 0.003666583311983284 86-87 0.0 0.0 3.068270553960907E-5 2.29261175791959 0.003850679545220939 88-89 0.0 0.0 3.068270553960907E-5 2.5232383141080614 0.004034775778458594 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6030 0.0 40.43756 1 GTATCAA 9845 0.0 37.800873 1 TCAACGC 11990 0.0 30.555082 4 ATCAACG 12045 0.0 30.41603 3 GTACATG 16065 0.0 29.796513 1 CAACGCA 12375 0.0 29.527985 5 TACATGG 16060 0.0 29.471088 2 TATCAAC 12730 0.0 28.963482 2 AACGCAG 12710 0.0 28.933693 6 ACATGGG 16935 0.0 26.971836 3 CATGGGG 8845 0.0 24.348671 4 ACGCAGA 14990 0.0 24.280993 7 CGCAGAG 15440 0.0 23.4828 8 GTATACG 165 2.501165E-6 22.956257 1 GTATAGG 765 0.0 22.281073 1 GTGTACG 440 0.0 21.52149 1 CATGGGA 7895 0.0 21.463106 4 GAGTACA 12395 0.0 20.894686 1 AGTACAT 11590 0.0 20.745409 2 GCAGAGT 17405 0.0 20.478083 9 >>END_MODULE